AlfalfaGEDB | MsG0380014027.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014027.01

KEGG: K03046
GO: GO:0003677 GO:0003899 GO:0006351
PFAM: PF00623 PF04983 PF04997 PF05000

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014027.01.T01	KAH0644102.1	92.513	561	29	2	61	610	69	627	0	1063

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014027.01.T01	Q8HVY4	96.577	409	14	0	20	428	142	550	0	818

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014027.01.T01	A0A6A5L4T6	89.539	564	33	1	73	610	57	620	0.0	1025

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048691.01	MsG0380014027.01	0.812916	3.332339e-51	2.145522e-48
MsG0280007042.01	MsG0380014027.01	0.824060	9.903644e-54	8.677905e-51
MsG0280009733.01	MsG0380014027.01	0.860179	2.473445e-63	6.702284e-60
MsG0380013888.01	MsG0380014027.01	0.827691	1.360611e-54	1.323127e-51
MsG0380013890.01	MsG0380014027.01	0.801089	1.061523e-48	5.025290e-46
MsG0380013917.01	MsG0380014027.01	0.833977	3.911801e-56	4.578896e-53
MsG0380013918.01	MsG0380014027.01	0.866358	3.023763e-65	1.016233e-61
MsG0380013926.01	MsG0380014027.01	0.824270	8.840643e-54	7.792649e-51
MsG0380013931.01	MsG0380014027.01	0.804071	2.575620e-49	1.315596e-46
MsG0380013935.01	MsG0380014027.01	0.817966	2.506701e-52	1.852063e-49

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014027.01.T01	MTR_0002s0510	80.163	615	96	2	20	610	1243	1855	0.0	995
MsG0380014027.01.T01	MTR_4g051130	98.837	430	3	1	1	428	1199	1628	0.0	871
MsG0380014027.01.T01	MTR_3g062770	98.837	430	3	1	1	428	147	576	0.0	867
MsG0380014027.01.T01	MTR_3g062790	98.837	430	3	1	1	428	147	576	0.0	867
MsG0380014027.01.T01	MTR_8g016510	84.661	339	6	2	90	428	1	293	0.0	560
MsG0380014027.01.T01	MTR_8g016520	96.703	182	6	0	429	610	7	188	1.94e-122	362
MsG0380014027.01.T01	MTR_4g051120	99.451	182	1	0	429	610	7	188	1.50e-116	378
MsG0380014027.01.T01	MTR_3g047940	93.939	132	8	0	151	282	5	136	4.04e-82	254
MsG0380014027.01.T01	MTR_4g009210	83.516	91	15	0	128	218	1	91	4.81e-42	151
MsG0380014027.01.T01	MTR_4g009210	57.600	125	19	1	305	429	108	198	1.66e-33	128

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014027.01.T01	ATCG00180	92.176	409	30	1	20	428	142	548	0.0	781
MsG0380014027.01.T01	ATCG00170	96.154	182	7	0	429	610	3	184	8.71e-112	365
MsG0380014027.01.T01	AT5G60040	28.710	310	188	5	141	423	250	553	8.95e-29	123
MsG0380014027.01.T01	AT5G60040	28.710	310	188	5	141	423	260	563	1.18e-28	122
MsG0380014027.01.T01	AT4G35800	28.710	310	192	5	141	423	242	549	1.94e-24	109
MsG0380014027.01.T01	AT4G35800	28.710	310	192	5	141	423	242	549	1.94e-24	109
MsG0380014027.01.T01	AT3G57660	24.806	387	215	12	151	491	335	691	3.94e-18	89.7
MsG0380014027.01.T01	AT3G57660	24.806	387	215	12	151	491	348	704	4.27e-18	89.4
MsG0380014027.01.T01	AT1G63020	23.978	367	238	10	79	422	152	500	1.34e-14	78.2
MsG0380014027.01.T01	AT1G63020	23.978	367	238	10	79	422	152	500	1.34e-14	78.2

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Find 133 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGGTACAAGAAGCCG+TGG	0.599616	3:-48683251	MsG0380014027.01.T01:CDS
AAATACTTCAAGAAGTTATG+CGG	0.639489	3:-48682910	MsG0380014027.01.T01:CDS
AAATACTTTAGCCGGATGAG+AGG	0.622971	3:-48683880	None:intergenic
AAATATAGAACCAGAATGGA+TGG	0.566690	3:-48683455	MsG0380014027.01.T01:CDS
AAATCAATCATTTGTCCTTG+TGG	0.513235	3:+48681525	None:intergenic
AACAAGTAGATCTACACCAG+GGG	0.706918	3:-48683293	MsG0380014027.01.T01:CDS
AACCTCTTGTCTCCGGCTAT+TGG	0.290298	3:-48682674	MsG0380014027.01.T01:CDS
AACTTTGTTGTTCAGCATCT+TGG	0.334259	3:+48681775	None:intergenic
AAGCATATCTTGAGTTGGTA+CGG	0.543078	3:+48682640	None:intergenic
AATACTTCAAGAAGTTATGC+GGG	0.538847	3:-48682909	MsG0380014027.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGAAATATGACCAACAG+TGG	+	Chr3:48683043-48683062	None:intergenic	30.0%
AAATATAGAACCAGAATGGA+TGG	-	Chr3:48681844-48681863	MsG0380014027.01.T01:CDS	30.0%
AAATCAATCATTTGTCCTTG+TGG	+	Chr3:48683777-48683796	None:intergenic	30.0%
AATACTTCAAGAAGTTATGC+GGG	-	Chr3:48682390-48682409	MsG0380014027.01.T01:intron	30.0%
ACTGCTACATCTATTTCATT+AGG	-	Chr3:48683459-48683478	MsG0380014027.01.T01:CDS	30.0%
AGACCGATTATTCAAATAGA+TGG	-	Chr3:48681902-48681921	MsG0380014027.01.T01:CDS	30.0%
AGCAAACGAAAAATTTGGAT+AGG	+	Chr3:48681492-48681511	None:intergenic	30.0%
AGCAGATTAATAGACCATTT+CGG	-	Chr3:48683378-48683397	MsG0380014027.01.T01:CDS	30.0%
AGTTGCTAAGAGTAAAATAC+GGG	-	Chr3:48682345-48682364	MsG0380014027.01.T01:intron	30.0%
ATATACATTAGAACCACTGT+TGG	-	Chr3:48683027-48683046	MsG0380014027.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48681434	48683887	48681434	ID=MsG0380014027.01;Name=MsG0380014027.01
Chr3	mRNA	48681434	48683887	48681434	ID=MsG0380014027.01.T01;Parent=MsG0380014027.01;Name=MsG0380014027.01.T01;_AED=0.50;_eAED=0.66;_QI=0\|0\|0\|1\|1\|1\|2\|0\|618
Chr3	exon	48682603	48683887	48682603	ID=MsG0380014027.01.T01:exon:56981;Parent=MsG0380014027.01.T01
Chr3	exon	48681434	48682005	48681434	ID=MsG0380014027.01.T01:exon:56980;Parent=MsG0380014027.01.T01
Chr3	CDS	48682603	48683887	48682603	ID=MsG0380014027.01.T01:cds;Parent=MsG0380014027.01.T01
Chr3	CDS	48681434	48682005	48681434	ID=MsG0380014027.01.T01:cds;Parent=MsG0380014027.01.T01

Gene Sequence

>MsG0380014027.01.T01

ATGAGAGGAAACCCTCACGTCCGATTTTCAAAGGGGGAGATCCATCTTATTGGATCCTATCCAAATTTTTCGTTTGCTAGGCCCGTAGTTAAAAAACCTACTTTCTTACGATTACGAGGTTCATTCGAATATGAAATCCAATCTTGGAAATACAGTATCCCACTCTTTTTTGCTACTCAAGGTTTCGATACATTTCGAAATAGAGAAATTTCTAGTGGAGCAGGTGCTATACGAGAACAATTAGTTGATCTGGATTTAAGAATTATTATGGATTCTTCGTTGGTAGAATGGAAAGAATTAGGAGAAGAGGGATCCACTGACAATGAAAATGAATGGGAAGATCGAAAAGTAGGAAGAAGAAAGAATTTTTTGGTTCGACGCATGGAATTAGCTAAGCACTTTATCCGAACAAATATAGAACCAGAATGGATGGTTTTATGTCTCTTACCAGTTCTTCCTCCCGAATTGAGACCGATTATTCAAATAGATGGAGGGAAACTAATGAGTTCGGATATTAATGAACTCTATAGAAGAGTTATCTATCGGAACAATACTCTTATTGACCTATTAACAACAAGTAGATCTACACCAGGGGAATTAGTAATGTGTCAAGAAAAATTGGTACAAGAAGCCGTGGATACACTCCTTGATAATGGAATCCGCGGACAACCAATGAGGGACGGTCATAATAAAGTTTACAAATCGTTTTCAGATATAATTGAGGGCAAAGAGGGAAGATTTCGAGAGACTCTGCTTGGAAAACGAGTTGATTATTCGGGGCGTTCTGTTATTGTCGTAGGTCCATCACTTTCATTACATCGATGTGGATTGCCCCGCGAAATAGCAATAGAGCTATTTCAGACATTTCTAATTCGTGGTTTAATTCGAAAACATTTTGCTTCGAACATAGGAGTTGCTAAGAGTAAAATACGGGAAAAAGAACCGATTGTATGGGAAATACTTCAAGAAGTTATGCGGGGGCATCCAGTATTGCTGAATAGAGCGCCTACTTTGCATAGATTGGGCATACAGGCATTTCAACCTATTTTAGTAGAAGGACGTGCTATTTGTTTACATCCATTAGTGTGTAAGGGATTCAATGCAGACTTTGATGGGGATCAAATGGCTGTTCATGTGCCTTTATCTTTGGAGGCTCAAGCAGAAGCTCGTTTACTTATGTTTTCTCATACAAACCTCTTGTCTCCGGCTATTGGGGATCCCATTTCCGTACCAACTCAAGATATGCTTATAGGCCTTTATGTATTAACTAGCGGAAATCGTCGAGAACGGGCCAATCTAGTCTTTCACAATAACGTGATAGGTGGAACTGCCATTAAACGACTTATTAGCAGATTAATAGACCATTTCGGAATGGCATATACATCACACATCCTGGATCAAGTAAAGACTCTAGGGTTCCGTCAAGCTACTGCTACATCTATTTCATTAGGAATTGATGATCTTTTAACAATACCTTCTAAAGGATGGCTAGTCCAAGATGCTGAACAACAAAGTTCTATTTTGGAAAAACATAATCAGTATGGGAATGTACATGCGGTAGAAAAATTACGCCAATCCATTGAAATATGGTATGCTACAAGCGAATATTTGCGACAAGAAATGAATCCTAATTTTAGGATGACTGACCCCTTTAATCCAGTCCATATAATGTCTTTTTCAGGAGCTAGAGGAAATGCATCTCAAGTGCACCAATTAGTCGGAATGAGAGGATTAATGTCAGATCCACAAGGACAAATGATTGATTTACCCATTCAAAGCAATTTACGAGAAGGACTGTCTTTAACAGAATATATAATTTCTTGCTACGGAGATATACTCTTTCTAGCTAAAGAATGA

Protein sequence

>MsG0380014027.01.T01

MRGNPHVRFSKGEIHLIGSYPNFSFARPVVKKPTFLRLRGSFEYEIQSWKYSIPLFFATQGFDTFRNREISSGAGAIREQLVDLDLRIIMDSSLVEWKELGEEGSTDNENEWEDRKVGRRKNFLVRRMELAKHFIRTNIEPEWMVLCLLPVLPPELRPIIQIDGGKLMSSDINELYRRVIYRNNTLIDLLTTSRSTPGELVMCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDIIEGKEGRFRETLLGKRVDYSGRSVIVVGPSLSLHRCGLPREIAIELFQTFLIRGLIRKHFASNIGVAKSKIREKEPIVWEILQEVMRGHPVLLNRAPTLHRLGIQAFQPILVEGRAICLHPLVCKGFNADFDGDQMAVHVPLSLEAQAEARLLMFSHTNLLSPAIGDPISVPTQDMLIGLYVLTSGNRRERANLVFHNNVIGGTAIKRLISRLIDHFGMAYTSHILDQVKTLGFRQATATSISLGIDDLLTIPSKGWLVQDAEQQSSILEKHNQYGNVHAVEKLRQSIEIWYATSEYLRQEMNPNFRMTDPFNPVHIMSFSGARGNASQVHQLVGMRGLMSDPQGQMIDLPIQSNLREGLSLTEYIISCYGDILFLAKE*