AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013890.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013890.01.T01 MTR_4g006040 97.849 93 2 0 6 98 145 237 2.63e-62 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAGATCACCTCCTTCATTC+TGG 0.237719 3:+47526571 MsG0380013890.01.T01:CDS
TCGTGGGATCCGGGCGGTCC+AGG 0.255275 3:+47526398 MsG0380013890.01.T01:CDS
GAGGATAGATGGGGCGATTC+AGG 0.280696 3:+47526342 MsG0380013890.01.T01:CDS
TTCTATTCACTCGTGGGATC+CGG 0.285661 3:+47526388 MsG0380013890.01.T01:CDS
ATATATCCCTTATCAGTATA+TGG 0.331456 3:+47526456 MsG0380013890.01.T01:CDS
TATCTCTCGAGCACAGGTTT+AGG 0.344757 3:+47526484 MsG0380013890.01.T01:CDS
CTTCTCCTCAGGAGGATAGA+TGG 0.346630 3:+47526331 MsG0380013890.01.T01:CDS
TATCCTCCTGAGGAGAAGTT+TGG 0.356300 3:-47526326 None:intergenic
GTGGGATCCGGGCGGTCCAG+GGG 0.385871 3:+47526400 MsG0380013890.01.T01:CDS
TGGGATCCGGGCGGTCCAGG+GGG 0.394291 3:+47526401 MsG0380013890.01.T01:CDS
CTCGAGCACAGGTTTAGGTT+TGG 0.395333 3:+47526489 MsG0380013890.01.T01:CDS
CGTGGGATCCGGGCGGTCCA+GGG 0.409711 3:+47526399 MsG0380013890.01.T01:CDS
GAGATCACCTCCTTCATTCT+GGG 0.425918 3:+47526572 MsG0380013890.01.T01:CDS
TTCTCCTCAGGAGGATAGAT+GGG 0.436019 3:+47526332 MsG0380013890.01.T01:CDS
GGAGGTGATCTCGTAGTTCT+TGG 0.438808 3:-47526561 None:intergenic
AGCTGTCCATATACTGATAA+GGG 0.448121 3:-47526462 None:intergenic
TAGCTGTCCATATACTGATA+AGG 0.448263 3:-47526463 None:intergenic
CTTCATTCTGGGGTGACGGC+GGG 0.455494 3:+47526583 MsG0380013890.01.T01:CDS
TTGAAACCAAACTTCTCCTC+AGG 0.463068 3:+47526320 None:intergenic
TCTATTCACTCGTGGGATCC+GGG 0.481377 3:+47526389 MsG0380013890.01.T01:CDS
GTCTGTGAAGATACGTTGTT+AGG 0.494777 3:-47526539 None:intergenic
GGGATCCGGGCGGTCCAGGG+GGG 0.502113 3:+47526402 MsG0380013890.01.T01:CDS
CCCACGAGTGAATAGAAAGT+TGG 0.515787 3:-47526382 None:intergenic
GACAGCTATCTCTCGAGCAC+AGG 0.526610 3:+47526478 MsG0380013890.01.T01:CDS
CCTTCATTCTGGGGTGACGG+CGG 0.526958 3:+47526582 MsG0380013890.01.T01:CDS
TCCAACTTTCTATTCACTCG+TGG 0.529895 3:+47526381 MsG0380013890.01.T01:CDS
CCGCCGTCACCCCAGAATGA+AGG 0.541532 3:-47526582 None:intergenic
CGAGAGGAGAGGAGCCATGA+TGG 0.546958 3:-47526426 None:intergenic
CCCATTTCGAGTCAAGAGAA+TGG 0.558119 3:-47526612 None:intergenic
ATAGAAAGTTGGATCTACAT+TGG 0.570959 3:-47526371 None:intergenic
CCTCCTTCATTCTGGGGTGA+CGG 0.571639 3:+47526579 MsG0380013890.01.T01:CDS
AAACCAAACTTCTCCTCAGG+AGG 0.577461 3:+47526323 None:intergenic
CCAACTTTCTATTCACTCGT+GGG 0.591847 3:+47526382 MsG0380013890.01.T01:CDS
CCGTCACCCCAGAATGAAGG+AGG 0.634091 3:-47526579 None:intergenic
ATATGAATTCTCGAGAGGAG+AGG 0.651842 3:-47526437 None:intergenic
GGGATATATGAATTCTCGAG+AGG 0.654907 3:-47526442 None:intergenic
TCGCCCCATCTATCCTCCTG+AGG 0.661903 3:-47526336 None:intergenic
AGATCACCTCCTTCATTCTG+GGG 0.666598 3:+47526573 MsG0380013890.01.T01:CDS
TCTCCTCAGGAGGATAGATG+GGG 0.682857 3:+47526333 MsG0380013890.01.T01:CDS
ATTCACTCGTGGGATCCGGG+CGG 0.763324 3:+47526392 MsG0380013890.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATATCCCTTATCAGTATA+TGG + Chr3:47526456-47526475 MsG0380013890.01.T01:CDS 25.0%
! CCTCAATAGAAAAAGAAAAA+CGG + Chr3:47526512-47526531 MsG0380013890.01.T01:CDS 25.0%
ATAGAAAGTTGGATCTACAT+TGG - Chr3:47526374-47526393 None:intergenic 30.0%
!! CCGTTTTTCTTTTTCTATTG+AGG - Chr3:47526515-47526534 None:intergenic 30.0%
AGCTGTCCATATACTGATAA+GGG - Chr3:47526465-47526484 None:intergenic 35.0%
TAGCTGTCCATATACTGATA+AGG - Chr3:47526466-47526485 None:intergenic 35.0%
ATATGAATTCTCGAGAGGAG+AGG - Chr3:47526440-47526459 None:intergenic 40.0%
CCAACTTTCTATTCACTCGT+GGG + Chr3:47526382-47526401 MsG0380013890.01.T01:CDS 40.0%
GGGATATATGAATTCTCGAG+AGG - Chr3:47526445-47526464 None:intergenic 40.0%
GTCTGTGAAGATACGTTGTT+AGG - Chr3:47526542-47526561 None:intergenic 40.0%
TCCAACTTTCTATTCACTCG+TGG + Chr3:47526381-47526400 MsG0380013890.01.T01:CDS 40.0%
AGATCACCTCCTTCATTCTG+GGG + Chr3:47526573-47526592 MsG0380013890.01.T01:CDS 45.0%
CCCACGAGTGAATAGAAAGT+TGG - Chr3:47526385-47526404 None:intergenic 45.0%
GAGATCACCTCCTTCATTCT+GGG + Chr3:47526572-47526591 MsG0380013890.01.T01:CDS 45.0%
TATCTCTCGAGCACAGGTTT+AGG + Chr3:47526484-47526503 MsG0380013890.01.T01:CDS 45.0%
TTCTATTCACTCGTGGGATC+CGG + Chr3:47526388-47526407 MsG0380013890.01.T01:CDS 45.0%
TTCTCCTCAGGAGGATAGAT+GGG + Chr3:47526332-47526351 MsG0380013890.01.T01:CDS 45.0%
CGAGATCACCTCCTTCATTC+TGG + Chr3:47526571-47526590 MsG0380013890.01.T01:CDS 50.0%
CTTCTCCTCAGGAGGATAGA+TGG + Chr3:47526331-47526350 MsG0380013890.01.T01:CDS 50.0%
GGAGGTGATCTCGTAGTTCT+TGG - Chr3:47526564-47526583 None:intergenic 50.0%
TCTATTCACTCGTGGGATCC+GGG + Chr3:47526389-47526408 MsG0380013890.01.T01:CDS 50.0%
TCTCCTCAGGAGGATAGATG+GGG + Chr3:47526333-47526352 MsG0380013890.01.T01:CDS 50.0%
! CTCGAGCACAGGTTTAGGTT+TGG + Chr3:47526489-47526508 MsG0380013890.01.T01:CDS 50.0%
GACAGCTATCTCTCGAGCAC+AGG + Chr3:47526478-47526497 MsG0380013890.01.T01:CDS 55.0%
GAGGATAGATGGGGCGATTC+AGG + Chr3:47526342-47526361 MsG0380013890.01.T01:CDS 55.0%
!! CCTCCTTCATTCTGGGGTGA+CGG + Chr3:47526579-47526598 MsG0380013890.01.T01:CDS 55.0%
ATTCACTCGTGGGATCCGGG+CGG + Chr3:47526392-47526411 MsG0380013890.01.T01:CDS 60.0%
CCGTCACCCCAGAATGAAGG+AGG - Chr3:47526582-47526601 None:intergenic 60.0%
CGAGAGGAGAGGAGCCATGA+TGG - Chr3:47526429-47526448 None:intergenic 60.0%
TCGCCCCATCTATCCTCCTG+AGG - Chr3:47526339-47526358 None:intergenic 60.0%
!! CCTTCATTCTGGGGTGACGG+CGG + Chr3:47526582-47526601 MsG0380013890.01.T01:CDS 60.0%
!! CTTCATTCTGGGGTGACGGC+GGG + Chr3:47526583-47526602 MsG0380013890.01.T01:CDS 60.0%
CCGCCGTCACCCCAGAATGA+AGG - Chr3:47526585-47526604 None:intergenic 65.0%
CGGTCCAGGGGGGACCATCA+TGG + Chr3:47526412-47526431 MsG0380013890.01.T01:CDS 70.0%
GGAGCCATGATGGTCCCCCC+TGG - Chr3:47526419-47526438 None:intergenic 70.0%
ATGGTCCCCCCTGGACCGCC+CGG - Chr3:47526410-47526429 None:intergenic 75.0%
CGTGGGATCCGGGCGGTCCA+GGG + Chr3:47526399-47526418 MsG0380013890.01.T01:CDS 75.0%
GTGGGATCCGGGCGGTCCAG+GGG + Chr3:47526400-47526419 MsG0380013890.01.T01:CDS 75.0%
TCGTGGGATCCGGGCGGTCC+AGG + Chr3:47526398-47526417 MsG0380013890.01.T01:CDS 75.0%
TGGGATCCGGGCGGTCCAGG+GGG + Chr3:47526401-47526420 MsG0380013890.01.T01:CDS 75.0%
! GGGATCCGGGCGGTCCAGGG+GGG + Chr3:47526402-47526421 MsG0380013890.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr3 gene 47526327 47526623 47526327 ID=MsG0380013890.01;Name=MsG0380013890.01
Chr3 mRNA 47526327 47526623 47526327 ID=MsG0380013890.01.T01;Parent=MsG0380013890.01;Name=MsG0380013890.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|98
Chr3 exon 47526327 47526623 47526327 ID=MsG0380013890.01.T01:exon:26400;Parent=MsG0380013890.01.T01
Chr3 CDS 47526327 47526623 47526327 ID=MsG0380013890.01.T01:cds;Parent=MsG0380013890.01.T01
Chr3 mRNA 47526431 47526505 47526431 ID=MsG0380013890.01.T02;Parent=MsG0380013890.01;Name=MsG0380013890.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|24
Chr3 exon 47526431 47526505 47526431 ID=MsG0380013890.01.T02:exon:26401;Parent=MsG0380013890.01.T02
Chr3 CDS 47526431 47526505 47526431 ID=MsG0380013890.01.T02:cds;Parent=MsG0380013890.01.T02
Gene Sequence

>MsG0380013890.01.T02

ATGGCTCCTCTCCTCTCGAGAATTCATATATCCCTTATCAGTATATGGACAGCTATCTCTCGAGCACAGGTTTAG

>MsG0380013890.01.T01

CAAACTTCTCCTCAGGAGGATAGATGGGGCGATTCAGGTGAGATCCAATGTAGATCCAACTTTCTATTCACTCGTGGGATCCGGGCGGTCCAGGGGGGACCATCATGGCTCCTCTCCTCTCGAGAATTCATATATCCCTTATCAGTATATGGACAGCTATCTCTCGAGCACAGGTTTAGGTTTGGCCTCAATAGAAAAAGAAAAACGGAGCACCTAACAACGTATCTTCACAGACCAAGAACTACGAGATCACCTCCTTCATTCTGGGGTGACGGCGGGATCGTACCATTCTCTTGA

Protein sequence

>MsG0380013890.01.T02

MAPLLSRIHISLISIWTAISRAQV*

>MsG0380013890.01.T01

QTSPQEDRWGDSGEIQCRSNFLFTRGIRAVQGGPSWLLSSREFIYPLSVYGQLSLEHRFRFGLNRKRKTEHLTTYLHRPRTTRSPPSFWGDGGIVPFS*