Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014580.01.T01 | XP_039687193.1 | 70.968 | 62 | 15 | 1 | 7 | 68 | 606 | 664 | 3.92E-15 | 79.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014580.01.T01 | A0A396IRW3 | 70.968 | 62 | 15 | 1 | 7 | 68 | 606 | 664 | 1.87e-15 | 79.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004398.01 | MsG0380014580.01 | 0.804153 | 2.477084e-49 | 1.267872e-46 |
| MsG0180005334.01 | MsG0380014580.01 | 0.808636 | 2.810418e-50 | 1.615147e-47 |
| MsG0180005993.01 | MsG0380014580.01 | 0.803002 | 4.290115e-49 | 2.131820e-46 |
| MsG0280006422.01 | MsG0380014580.01 | 0.817890 | 2.607513e-52 | 1.922576e-49 |
| MsG0380014580.01 | MsG0580028044.01 | 0.801683 | 8.021823e-49 | 3.854553e-46 |
| MsG0380014580.01 | MsG0680035888.01 | 0.804755 | 1.854791e-49 | 9.642477e-47 |
| MsG0380014580.01 | MsG0780038400.01 | 0.803092 | 4.111273e-49 | 2.047600e-46 |
| MsG0280007727.01 | MsG0380014580.01 | 0.806199 | 9.235306e-50 | 4.982422e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014580.01.T01 | AT5G55960 | 59.259 | 54 | 19 | 1 | 7 | 60 | 595 | 645 | 7.64e-12 | 58.9 |
Find 12 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTCTTGGCCCTGTCCTT+TGG | 0.304822 | 3:-56703331 | None:intergenic |
| TTTATATGCTGAATTTGTTT+TGG | 0.310605 | 3:+56703303 | MsG0380014580.01.T01:CDS |
| TGAAAGGTATGTTGTTCTGC+AGG | 0.488546 | 3:+56703231 | MsG0380014580.01.T01:CDS |
| ATCATGACTGTAGTTATCAA+TGG | 0.519786 | 3:-56703268 | None:intergenic |
| ACGCTGTTAGATTGACTGAA+AGG | 0.580827 | 3:+56703215 | MsG0380014580.01.T01:CDS |
| AAAGGTATGTTGTTCTGCAG+GGG | 0.605195 | 3:+56703233 | MsG0380014580.01.T01:CDS |
| GAAAGGTATGTTGTTCTGCA+GGG | 0.605290 | 3:+56703232 | MsG0380014580.01.T01:CDS |
| CAGTCAATCTAACAGCGTCA+TGG | 0.610914 | 3:-56703210 | None:intergenic |
| TTTGGAAGAACCAAAGGACA+GGG | 0.620836 | 3:+56703321 | MsG0380014580.01.T01:CDS |
| AAGGTATGTTGTTCTGCAGG+GGG | 0.670597 | 3:+56703234 | MsG0380014580.01.T01:CDS |
| AAACACAGTCTATTTCCACA+TGG | 0.715308 | 3:-56702841 | None:intergenic |
| TAAATGATGCGGTGTCCATG+TGG | 0.718158 | 3:+56702826 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAACACAATTAAAAAACAA+AGG | - | Chr3:56703139-56703158 | None:intergenic | 15.0% |
| !!! | TTTATATGCTGAATTTGTTT+TGG | + | Chr3:56703303-56703322 | MsG0380014580.01.T01:CDS | 20.0% |
| ! | ACTGTTTCCATATTAGTTAT+AGG | + | Chr3:56703055-56703074 | MsG0380014580.01.T01:intron | 25.0% |
| !! | TTTAATCAGATATTTTCCAG+TGG | - | Chr3:56702945-56702964 | None:intergenic | 25.0% |
| ATCATGACTGTAGTTATCAA+TGG | - | Chr3:56703271-56703290 | None:intergenic | 30.0% | |
| GACATAGCCTATAACTAATA+TGG | - | Chr3:56703065-56703084 | None:intergenic | 30.0% | |
| ! | TTTCTGAAAATGACCACAAT+TGG | - | Chr3:56702908-56702927 | None:intergenic | 30.0% |
| !!! | ATTTGTTTTGGAAGAACCAA+AGG | + | Chr3:56703315-56703334 | MsG0380014580.01.T01:CDS | 30.0% |
| AAACACAGTCTATTTCCACA+TGG | - | Chr3:56702844-56702863 | None:intergenic | 35.0% | |
| AAAGATAAACCAAAAGCCAC+TGG | + | Chr3:56702926-56702945 | MsG0380014580.01.T01:intron | 35.0% | |
| ! | AGATATTTTCCAGTGGCTTT+TGG | - | Chr3:56702938-56702957 | None:intergenic | 35.0% |
| ! | ATAGACTGTGTTTTCCACAA+AGG | + | Chr3:56702851-56702870 | MsG0380014580.01.T01:intron | 35.0% |
| ! | TAGACTGTGTTTTCCACAAA+GGG | + | Chr3:56702852-56702871 | MsG0380014580.01.T01:intron | 35.0% |
| ACGCTGTTAGATTGACTGAA+AGG | + | Chr3:56703215-56703234 | MsG0380014580.01.T01:CDS | 40.0% | |
| GACTGACGAAGTTATAGAGA+GGG | + | Chr3:56702881-56702900 | MsG0380014580.01.T01:intron | 40.0% | |
| GTCAGTCAAAAATCCCTTTG+TGG | - | Chr3:56702868-56702887 | None:intergenic | 40.0% | |
| TGACTGACGAAGTTATAGAG+AGG | + | Chr3:56702880-56702899 | MsG0380014580.01.T01:intron | 40.0% | |
| TTATAGAGAGGGACCAATTG+TGG | + | Chr3:56702892-56702911 | MsG0380014580.01.T01:intron | 40.0% | |
| TTTGGAAGAACCAAAGGACA+GGG | + | Chr3:56703321-56703340 | MsG0380014580.01.T01:CDS | 40.0% | |
| ! | AAAGGTATGTTGTTCTGCAG+GGG | + | Chr3:56703233-56703252 | MsG0380014580.01.T01:CDS | 40.0% |
| ! | GAAAGGTATGTTGTTCTGCA+GGG | + | Chr3:56703232-56703251 | MsG0380014580.01.T01:CDS | 40.0% |
| ! | TGAAAGGTATGTTGTTCTGC+AGG | + | Chr3:56703231-56703250 | MsG0380014580.01.T01:CDS | 40.0% |
| !! | TTTTGGAAGAACCAAAGGAC+AGG | + | Chr3:56703320-56703339 | MsG0380014580.01.T01:CDS | 40.0% |
| AAGGTATGTTGTTCTGCAGG+GGG | + | Chr3:56703234-56703253 | MsG0380014580.01.T01:CDS | 45.0% | |
| CAGTCAATCTAACAGCGTCA+TGG | - | Chr3:56703213-56703232 | None:intergenic | 45.0% | |
| GTTCTGCAGGGGGCTATTAT+GGG | + | Chr3:56703244-56703263 | MsG0380014580.01.T01:CDS | 50.0% | |
| TGTTCTGCAGGGGGCTATTA+TGG | + | Chr3:56703243-56703262 | MsG0380014580.01.T01:CDS | 50.0% | |
| TTCTGCAGGGGGCTATTATG+GGG | + | Chr3:56703245-56703264 | MsG0380014580.01.T01:CDS | 50.0% | |
| TTCTTCTTGGCCCTGTCCTT+TGG | - | Chr3:56703334-56703353 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 56702829 | 56703363 | 56702829 | ID=MsG0380014580.01;Name=MsG0380014580.01 |
| Chr3 | mRNA | 56702829 | 56703363 | 56702829 | ID=MsG0380014580.01.T01;Parent=MsG0380014580.01;Name=MsG0380014580.01.T01;_AED=0.49;_eAED=0.72;_QI=0|0|0|1|0|0|2|0|68 |
| Chr3 | exon | 56702829 | 56702857 | 56702829 | ID=MsG0380014580.01.T01:exon:23941;Parent=MsG0380014580.01.T01 |
| Chr3 | exon | 56703186 | 56703363 | 56703186 | ID=MsG0380014580.01.T01:exon:23942;Parent=MsG0380014580.01.T01 |
| Chr3 | CDS | 56702829 | 56702857 | 56702829 | ID=MsG0380014580.01.T01:cds;Parent=MsG0380014580.01.T01 |
| Chr3 | CDS | 56703186 | 56703363 | 56703186 | ID=MsG0380014580.01.T01:cds;Parent=MsG0380014580.01.T01 |
| Gene Sequence |
| Protein sequence |
