Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015561.01.T01 | XP_003601066.1 | 96.818 | 220 | 4 | 2 | 1 | 217 | 1 | 220 | 3.44E-147 | 418 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280009108.01 | MsG0380015561.01 | 0.810552 | 1.089382e-50 | 6.586447e-48 |
| MsG0280009139.01 | MsG0380015561.01 | 0.810138 | 1.337931e-50 | 8.001495e-48 |
| MsG0380015561.01 | MsG0380015862.01 | 0.813732 | 2.205459e-51 | 1.451803e-48 |
| MsG0380015561.01 | MsG0480021859.01 | 0.812046 | 5.162827e-51 | 3.247775e-48 |
| MsG0380015561.01 | MsG0580025441.01 | 0.852244 | 5.243858e-61 | 1.088941e-57 |
| MsG0380015561.01 | MsG0880045477.01 | 0.803209 | 3.887493e-49 | 1.942101e-46 |
| MsG0380015561.01 | MsG0880047494.01 | 0.851526 | 8.382140e-61 | 1.701394e-57 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015561.01.T01 | MTR_3g072610 | 96.818 | 220 | 4 | 2 | 1 | 217 | 1 | 220 | 4.17e-151 | 418 |
| MsG0380015561.01.T01 | MTR_5g058470 | 59.836 | 244 | 64 | 10 | 1 | 216 | 60 | 297 | 3.61e-61 | 193 |
| MsG0380015561.01.T01 | MTR_1g014800 | 35.784 | 204 | 108 | 8 | 23 | 217 | 29 | 218 | 1.66e-30 | 112 |
| MsG0380015561.01.T01 | MTR_1g014860 | 38.660 | 194 | 99 | 6 | 20 | 193 | 17 | 210 | 1.26e-29 | 110 |
| MsG0380015561.01.T01 | MTR_1g014820 | 55.435 | 92 | 40 | 1 | 20 | 110 | 22 | 113 | 5.28e-29 | 109 |
| MsG0380015561.01.T01 | MTR_1g014780 | 36.842 | 190 | 100 | 7 | 20 | 191 | 33 | 220 | 2.17e-28 | 107 |
| MsG0380015561.01.T01 | MTR_5g008790 | 53.933 | 89 | 39 | 1 | 23 | 109 | 16 | 104 | 1.12e-26 | 100 |
| MsG0380015561.01.T01 | MTR_3g105510 | 51.613 | 93 | 44 | 1 | 19 | 110 | 31 | 123 | 5.43e-26 | 101 |
| MsG0380015561.01.T01 | MTR_2g043050 | 51.327 | 113 | 42 | 5 | 1 | 111 | 1 | 102 | 2.03e-25 | 99.0 |
| MsG0380015561.01.T01 | MTR_7g046260 | 52.041 | 98 | 40 | 3 | 7 | 102 | 43 | 135 | 1.11e-24 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015561.01.T01 | AT1G21910 | 50.242 | 207 | 61 | 9 | 25 | 216 | 50 | 229 | 1.67e-47 | 156 |
| MsG0380015561.01.T01 | AT1G44830 | 51.707 | 205 | 63 | 9 | 23 | 217 | 33 | 211 | 1.64e-34 | 122 |
| MsG0380015561.01.T01 | AT1G77640 | 46.262 | 214 | 86 | 7 | 23 | 217 | 41 | 244 | 4.80e-32 | 117 |
| MsG0380015561.01.T01 | AT1G74930 | 49.038 | 104 | 50 | 2 | 11 | 114 | 7 | 107 | 8.24e-28 | 104 |
| MsG0380015561.01.T01 | AT4G06746 | 48.182 | 110 | 50 | 3 | 1 | 106 | 13 | 119 | 1.14e-26 | 100 |
| MsG0380015561.01.T01 | AT1G19210 | 34.615 | 208 | 100 | 7 | 18 | 216 | 5 | 185 | 6.35e-26 | 99.8 |
| MsG0380015561.01.T01 | AT2G44940 | 48.305 | 118 | 52 | 3 | 25 | 142 | 100 | 208 | 2.46e-25 | 100 |
| MsG0380015561.01.T01 | AT2G35700 | 46.617 | 133 | 59 | 5 | 20 | 149 | 40 | 163 | 2.52e-25 | 98.2 |
| MsG0380015561.01.T01 | AT2G23340 | 55.056 | 89 | 33 | 2 | 23 | 106 | 26 | 112 | 2.94e-25 | 97.4 |
| MsG0380015561.01.T01 | AT1G71520 | 39.735 | 151 | 75 | 5 | 14 | 160 | 3 | 141 | 8.00e-25 | 95.5 |
Find 27 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATCTGATCAACATATTT+AGG | 0.316828 | 3:-71882188 | None:intergenic |
| AAGAAAGTGACATTCCTTTG+TGG | 0.498615 | 3:+71882273 | MsG0380015561.01.T01:CDS |
| AAGGAGTAAGAATGAGGAGT+TGG | 0.457971 | 3:+71881730 | MsG0380015561.01.T01:CDS |
| AAGTGCAGCATCATAAGCTC+TGG | 0.543771 | 3:-71881835 | None:intergenic |
| AATGAGGAGTTGGGGTTCAT+GGG | 0.458646 | 3:+71881740 | MsG0380015561.01.T01:CDS |
| AATGTGAAGATGCTGTTAAA+GGG | 0.512314 | 3:-71881899 | None:intergenic |
| ACTAATTGGTATGGATTAGA+AGG | 0.299088 | 3:+71882155 | MsG0380015561.01.T01:CDS |
| AGGAGTAAGAATGAGGAGTT+GGG | 0.383877 | 3:+71881731 | MsG0380015561.01.T01:CDS |
| AGTACAAAGGAGTAAGAATG+AGG | 0.534167 | 3:+71881724 | MsG0380015561.01.T01:CDS |
| AGTAGCAAGAAGAAGTACAA+AGG | 0.601213 | 3:+71881711 | MsG0380015561.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGTGAAGATGCTGTTAAA+GGG | - | Chr3:71881902-71881921 | None:intergenic | 30.0% | |
| ATATCGGAAACTAATTGGTA+TGG | + | Chr3:71882146-71882165 | MsG0380015561.01.T01:CDS | 30.0% | |
| CTATGATATCGGAAACTAAT+TGG | + | Chr3:71882141-71882160 | MsG0380015561.01.T01:CDS | 30.0% | |
| TAATGTGAAGATGCTGTTAA+AGG | - | Chr3:71881903-71881922 | None:intergenic | 30.0% | |
| AAGAAAGTGACATTCCTTTG+TGG | + | Chr3:71882273-71882292 | MsG0380015561.01.T01:CDS | 35.0% | |
| AGTACAAAGGAGTAAGAATG+AGG | + | Chr3:71881724-71881743 | MsG0380015561.01.T01:CDS | 35.0% | |
| GATTCATCATCACATTTGCT+TGG | + | Chr3:71882236-71882255 | MsG0380015561.01.T01:CDS | 35.0% | |
| TCAGAAGAAACCATTGATGA+TGG | - | Chr3:71882068-71882087 | None:intergenic | 35.0% | |
| TCTAGTTAACGATGAATCGA+TGG | + | Chr3:71882118-71882137 | MsG0380015561.01.T01:CDS | 35.0% | |
| TGAATCGATGGCTATGATAT+CGG | + | Chr3:71882130-71882149 | MsG0380015561.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 71881654 | 71882307 | 71881654 | ID=MsG0380015561.01;Name=MsG0380015561.01 |
| Chr3 | mRNA | 71881654 | 71882307 | 71881654 | ID=MsG0380015561.01.T01;Parent=MsG0380015561.01;Name=MsG0380015561.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|217 |
| Chr3 | exon | 71881654 | 71882307 | 71881654 | ID=MsG0380015561.01.T01:exon:23850;Parent=MsG0380015561.01.T01 |
| Chr3 | CDS | 71881654 | 71882307 | 71881654 | ID=MsG0380015561.01.T01:cds;Parent=MsG0380015561.01.T01 |
| Gene Sequence |
>MsG0380015561.01.T01 ATGGTTAAGTCAGAGACTAACAAGATAAAAAAAGAACCATCAAATTCAATCTCAGATAGTAGCAAGAAGAAGTACAAAGGAGTAAGAATGAGGAGTTGGGGTTCATGGGTATCAGAGATTAGAGCACCAAATCAAAAAACAAGAATTTGGTTAGGTTCTTATTCAACTGCAGAAGCAGCAGCCAGAGCTTATGATGCTGCACTTTTATGTCTTAAAGGTTCATCATCTGCATCAAATCTCAATTTCCCTTTAACAGCATCTTCACATTACAATATTCCTCAAGATATGTCTCCGAAATCAATTCAAAGAGTTGCTGCAGCTGCCGCGAATAGTTTCACTGACAACAATAATGTTAATGTTAATGTCAATGATAATGTTAACACTCCCTCTTCATCATCATTAGTGTCATCTCCATCATCAATGGTTTCTTCTGATGATGTTTCTTCACTTATGTCATCTTTCGATCTAGTTAACGATGAATCGATGGCTATGATATCGGAAACTAATTGGTATGGATTAGAAGGTTTACAATCTCCTAAATATGTTGATCAGATGTTGTTAAGTGCTTCTTTCTTTGATATTGATTCATCATCACATTTGCTTGGTGATGATCTCTATGAAGAAAGTGACATTCCTTTGTGGAACTTCAGCTGA |
| Protein sequence |
>MsG0380015561.01.T01 MVKSETNKIKKEPSNSISDSSKKKYKGVRMRSWGSWVSEIRAPNQKTRIWLGSYSTAEAAARAYDAALLCLKGSSSASNLNFPLTASSHYNIPQDMSPKSIQRVAAAAANSFTDNNNVNVNVNDNVNTPSSSSLVSSPSSMVSSDDVSSLMSSFDLVNDESMAMISETNWYGLEGLQSPKYVDQMLLSASFFDIDSSSHLLGDDLYEESDIPLWNFS* |
