Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015862.01.T01 | ACJ85044.1 | 82.119 | 151 | 22 | 2 | 1 | 146 | 1 | 151 | 5.00E-83 | 252 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015862.01.T01 | Q9ZVD2 | 51.064 | 141 | 56 | 1 | 1 | 128 | 1 | 141 | 4.18E-44 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015862.01.T01 | B7FJW5 | 82.119 | 151 | 22 | 2 | 1 | 146 | 1 | 151 | 2.39e-83 | 252 |
| MsG0380015862.01.T01 | I3SF41 | 82.119 | 151 | 22 | 2 | 1 | 146 | 1 | 151 | 1.00e-82 | 254 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005199.01 | MsG0380015862.01 | 0.807309 | 5.382173e-50 | 2.987856e-47 |
| MsG0380015561.01 | MsG0380015862.01 | 0.813732 | 2.205459e-51 | 1.451803e-48 |
| MsG0380015676.01 | MsG0380015862.01 | 0.878304 | 3.130358e-69 | 1.626170e-65 |
| MsG0380015862.01 | MsG0480022592.01 | 0.800504 | 1.397159e-48 | 6.516312e-46 |
| MsG0380015862.01 | MsG0480022639.01 | 0.823287 | 1.502445e-53 | 1.288100e-50 |
| MsG0380015862.01 | MsG0580025441.01 | 0.832370 | 9.827602e-56 | 1.096201e-52 |
| MsG0380015862.01 | MsG0580027875.01 | 0.833787 | 4.363499e-56 | 5.078176e-53 |
| MsG0380015862.01 | MsG0580028371.01 | 0.868495 | 6.257244e-66 | 2.268150e-62 |
| MsG0380015862.01 | MsG0580028377.01 | 0.855382 | 6.551178e-62 | 1.509826e-58 |
| MsG0380015862.01 | MsG0580029205.01 | 0.817864 | 2.643694e-52 | 1.947888e-49 |
| MsG0380015862.01 | MsG0880045477.01 | 0.819285 | 1.258467e-52 | 9.642653e-50 |
| MsG0380015862.01 | MsG0880047123.01 | 0.816716 | 4.790523e-52 | 3.420417e-49 |
| MsG0380015862.01 | MsG0880047494.01 | 0.830841 | 2.340101e-55 | 2.494911e-52 |
| MsG0380015862.01 | MsG0880047495.01 | 0.825274 | 5.126414e-54 | 4.650900e-51 |
| MsG0380015862.01 | MsG0880047496.01 | 0.807367 | 5.231968e-50 | 2.908744e-47 |
| MsG0380015862.01 | MsG0880047554.01 | 0.901412 | 2.750099e-78 | 3.710687e-74 |
| MsG0280008007.01 | MsG0380015862.01 | 0.841209 | 5.459192e-58 | 7.963635e-55 |
| MsG0280009108.01 | MsG0380015862.01 | 0.825311 | 5.023933e-54 | 4.562704e-51 |
| MsG0280009139.01 | MsG0380015862.01 | 0.833433 | 5.348197e-56 | 6.158232e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015862.01.T01 | MTR_3g078800 | 82.119 | 151 | 22 | 2 | 1 | 146 | 1 | 151 | 2.08e-86 | 254 |
| MsG0380015862.01.T01 | MTR_5g087760 | 58.824 | 136 | 48 | 2 | 1 | 128 | 1 | 136 | 1.56e-53 | 170 |
| MsG0380015862.01.T01 | MTR_2g012550 | 42.568 | 148 | 67 | 2 | 1 | 130 | 1 | 148 | 1.07e-34 | 122 |
| MsG0380015862.01.T01 | MTR_3g078820 | 59.770 | 87 | 35 | 0 | 41 | 127 | 2 | 88 | 1.82e-19 | 81.6 |
| MsG0380015862.01.T01 | MTR_8g090320 | 37.500 | 112 | 57 | 4 | 24 | 128 | 43 | 148 | 1.24e-16 | 75.1 |
| MsG0380015862.01.T01 | MTR_3g074150 | 39.362 | 94 | 45 | 4 | 13 | 98 | 16 | 105 | 2.35e-15 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015862.01.T01 | AT2G27080 | 51.064 | 141 | 56 | 1 | 1 | 128 | 1 | 141 | 4.26e-45 | 149 |
| MsG0380015862.01.T01 | AT2G27080 | 51.064 | 141 | 56 | 1 | 1 | 128 | 1 | 141 | 4.26e-45 | 149 |
| MsG0380015862.01.T01 | AT5G21130 | 50.467 | 107 | 53 | 0 | 18 | 124 | 52 | 158 | 3.52e-33 | 119 |
Find 47 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGCTGTTGCTGTCTTTGC+TGG | 0.303551 | 3:+76039816 | MsG0380015862.01.T01:CDS |
| ATGCGGTAGGTTTGATCCTT+GGG | 0.329031 | 3:-76039731 | None:intergenic |
| ATTGTGTATTTCGGTGCTTC+CGG | 0.342584 | 3:-76039911 | None:intergenic |
| TTGGAAGGTGGAGAGGCGTT+AGG | 0.343094 | 3:-76039665 | None:intergenic |
| AGGTTTGATCCTTGGGGAGC+TGG | 0.388163 | 3:-76039724 | None:intergenic |
| CGCTGTGCATTCTCTGGTGG+TGG | 0.395822 | 3:-76039755 | None:intergenic |
| GTGCTCATGGCCATCGCTGC+CGG | 0.415051 | 3:+76039866 | MsG0380015862.01.T01:CDS |
| CGGCGGCGATGGAGCGGTGT+TGG | 0.430905 | 3:-76039684 | None:intergenic |
| GATGCGGTAGGTTTGATCCT+TGG | 0.434091 | 3:-76039732 | None:intergenic |
| GCGTAACGCTGTGCATTCTC+TGG | 0.448542 | 3:-76039761 | None:intergenic |
| ACCGCTCCATCGCCGCCGTC+CGG | 0.448630 | 3:+76039689 | MsG0380015862.01.T01:CDS |
| TGTTAGGTTCATTCCCTTGA+CGG | 0.454596 | 3:-76039945 | None:intergenic |
| ACACGATCGATTGTGTATTT+CGG | 0.457527 | 3:-76039920 | None:intergenic |
| TGCTGTCTTTGCTGGTTCAT+CGG | 0.470949 | 3:+76039824 | MsG0380015862.01.T01:CDS |
| AAATAGTAGATAAAGATGTT+AGG | 0.480050 | 3:-76039961 | None:intergenic |
| TGGAAGGTGGAGAGGCGTTA+GGG | 0.484091 | 3:-76039664 | None:intergenic |
| GTTTCTGTTAAAGCTGATAA+CGG | 0.484250 | 3:+76039998 | MsG0380015862.01.T01:CDS |
| GGCGATGGAGCGGTGTTGGA+AGG | 0.486020 | 3:-76039680 | None:intergenic |
| ATTTACTCTGATCGTGCTCA+TGG | 0.493417 | 3:+76039853 | MsG0380015862.01.T01:CDS |
| TCTCTGTAACGGCGTCCTGC+CGG | 0.493799 | 3:+76040086 | MsG0380015862.01.T01:CDS |
| CTCTGGTGGTGGGATGCGGT+AGG | 0.506358 | 3:-76039744 | None:intergenic |
| GAGCTGGATGACATATGTTC+CGG | 0.509609 | 3:-76039708 | None:intergenic |
| CATTCTCTGGTGGTGGGATG+CGG | 0.527360 | 3:-76039748 | None:intergenic |
| AATCGATCGTGTCTCCGTCA+AGG | 0.528158 | 3:+76039931 | MsG0380015862.01.T01:CDS |
| ATCGATCGTGTCTCCGTCAA+GGG | 0.528651 | 3:+76039932 | MsG0380015862.01.T01:CDS |
| ACGGCTGATAAAACGCCGGC+AGG | 0.534301 | 3:-76040101 | None:intergenic |
| TATGTTCCGGACGGCGGCGA+TGG | 0.535007 | 3:-76039695 | None:intergenic |
| TCCGGACGGCGGCGATGGAG+CGG | 0.543871 | 3:-76039690 | None:intergenic |
| GGGTGAACACGATCAGCCAT+TGG | 0.548503 | 3:-76039644 | None:intergenic |
| GACAGCAACAGCATCCGCAC+CGG | 0.556268 | 3:-76039808 | None:intergenic |
| TGATAACGGTAACAAAAGAT+CGG | 0.585074 | 3:+76040012 | MsG0380015862.01.T01:CDS |
| GATGGAGCGGTGTTGGAAGG+TGG | 0.592952 | 3:-76039677 | None:intergenic |
| AGCGGTGTTGGAAGGTGGAG+AGG | 0.599059 | 3:-76039672 | None:intergenic |
| CGCCGCAAAAGTCGCCGGTG+CGG | 0.603490 | 3:+76039794 | MsG0380015862.01.T01:CDS |
| ACACTCGCCGCAAAAGTCGC+CGG | 0.610216 | 3:+76039789 | MsG0380015862.01.T01:CDS |
| TTTAACAGAAACATCAAACT+CGG | 0.615677 | 3:-76039987 | None:intergenic |
| GCTGTGCATTCTCTGGTGGT+GGG | 0.616243 | 3:-76039754 | None:intergenic |
| TAACGCTGTGCATTCTCTGG+TGG | 0.616606 | 3:-76039758 | None:intergenic |
| TGCGGTAGGTTTGATCCTTG+GGG | 0.618357 | 3:-76039730 | None:intergenic |
| AACACCGTCACGTTATTCGA+CGG | 0.636233 | 3:-76040120 | None:intergenic |
| TTAACAGAAACATCAAACTC+GGG | 0.650957 | 3:-76039986 | None:intergenic |
| AGAGGCGTTAGTCTCTGTAA+CGG | 0.674629 | 3:+76040075 | MsG0380015862.01.T01:CDS |
| ATATGTCATCCAGCTCCCCA+AGG | 0.678653 | 3:+76039715 | MsG0380015862.01.T01:CDS |
| ATGACATATGTTCCGGACGG+CGG | 0.681432 | 3:-76039701 | None:intergenic |
| TGGATGACATATGTTCCGGA+CGG | 0.702336 | 3:-76039704 | None:intergenic |
| TTCGACGGCTGATAAAACGC+CGG | 0.712718 | 3:-76040105 | None:intergenic |
| TCAGCCGTCGAATAACGTGA+CGG | 0.740743 | 3:+76040116 | MsG0380015862.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAGTAGATAAAGATGTT+AGG | - | Chr3:76039964-76039983 | None:intergenic | 20.0% |
| ! | TTTAACAGAAACATCAAACT+CGG | - | Chr3:76039990-76040009 | None:intergenic | 25.0% |
| AACGATCAAGTAAAAAACAC+CGG | - | Chr3:76039888-76039907 | None:intergenic | 30.0% | |
| GTTTCTGTTAAAGCTGATAA+CGG | + | Chr3:76039998-76040017 | MsG0380015862.01.T01:CDS | 30.0% | |
| TTAACAGAAACATCAAACTC+GGG | - | Chr3:76039989-76040008 | None:intergenic | 30.0% | |
| !! | TGATAACGGTAACAAAAGAT+CGG | + | Chr3:76040012-76040031 | MsG0380015862.01.T01:CDS | 30.0% |
| ACACGATCGATTGTGTATTT+CGG | - | Chr3:76039923-76039942 | None:intergenic | 35.0% | |
| ! | TTTTTACTTGATCGTTCGTC+CGG | + | Chr3:76039892-76039911 | MsG0380015862.01.T01:CDS | 35.0% |
| !! | AGCGTTGAAATGTTTTACAG+AGG | + | Chr3:76040057-76040076 | MsG0380015862.01.T01:CDS | 35.0% |
| ATTTACTCTGATCGTGCTCA+TGG | + | Chr3:76039853-76039872 | MsG0380015862.01.T01:CDS | 40.0% | |
| TGTTAGGTTCATTCCCTTGA+CGG | - | Chr3:76039948-76039967 | None:intergenic | 40.0% | |
| !! | ATTGTGTATTTCGGTGCTTC+CGG | - | Chr3:76039914-76039933 | None:intergenic | 40.0% |
| ATGCGGTAGGTTTGATCCTT+GGG | - | Chr3:76039734-76039753 | None:intergenic | 45.0% | |
| GAGCTGGATGACATATGTTC+CGG | - | Chr3:76039711-76039730 | None:intergenic | 45.0% | |
| TGGATGACATATGTTCCGGA+CGG | - | Chr3:76039707-76039726 | None:intergenic | 45.0% | |
| !! | AGAGGCGTTAGTCTCTGTAA+CGG | + | Chr3:76040075-76040094 | MsG0380015862.01.T01:CDS | 45.0% |
| !! | TGCTGTCTTTGCTGGTTCAT+CGG | + | Chr3:76039824-76039843 | MsG0380015862.01.T01:CDS | 45.0% |
| AATCGATCGTGTCTCCGTCA+AGG | + | Chr3:76039931-76039950 | MsG0380015862.01.T01:CDS | 50.0% | |
| ATATGTCATCCAGCTCCCCA+AGG | + | Chr3:76039715-76039734 | MsG0380015862.01.T01:CDS | 50.0% | |
| ATCGATCGTGTCTCCGTCAA+GGG | + | Chr3:76039932-76039951 | MsG0380015862.01.T01:CDS | 50.0% | |
| ATGACATATGTTCCGGACGG+CGG | - | Chr3:76039704-76039723 | None:intergenic | 50.0% | |
| GATGCGGTAGGTTTGATCCT+TGG | - | Chr3:76039735-76039754 | None:intergenic | 50.0% | |
| GATGCTGTTGCTGTCTTTGC+TGG | + | Chr3:76039816-76039835 | MsG0380015862.01.T01:CDS | 50.0% | |
| GTAAAAAACACCGGCAGCGA+TGG | - | Chr3:76039879-76039898 | None:intergenic | 50.0% | |
| TAACGCTGTGCATTCTCTGG+TGG | - | Chr3:76039761-76039780 | None:intergenic | 50.0% | |
| TGCGGTAGGTTTGATCCTTG+GGG | - | Chr3:76039733-76039752 | None:intergenic | 50.0% | |
| TTCGACGGCTGATAAAACGC+CGG | - | Chr3:76040108-76040127 | None:intergenic | 50.0% | |
| ! | ACTTTTGCGGCGAGTGTAGT+TGG | - | Chr3:76039786-76039805 | None:intergenic | 50.0% |
| AGGTTTGATCCTTGGGGAGC+TGG | - | Chr3:76039727-76039746 | None:intergenic | 55.0% | |
| GCGTAACGCTGTGCATTCTC+TGG | - | Chr3:76039764-76039783 | None:intergenic | 55.0% | |
| GCTGTGCATTCTCTGGTGGT+GGG | - | Chr3:76039757-76039776 | None:intergenic | 55.0% | |
| !! | CATTCTCTGGTGGTGGGATG+CGG | - | Chr3:76039751-76039770 | None:intergenic | 55.0% |
| !! | TGGAAGGTGGAGAGGCGTTA+GGG | - | Chr3:76039667-76039686 | None:intergenic | 55.0% |
| !! | TTGGAAGGTGGAGAGGCGTT+AGG | - | Chr3:76039668-76039687 | None:intergenic | 55.0% |
| ACACTCGCCGCAAAAGTCGC+CGG | + | Chr3:76039789-76039808 | MsG0380015862.01.T01:CDS | 60.0% | |
| ACGGCTGATAAAACGCCGGC+AGG | - | Chr3:76040104-76040123 | None:intergenic | 60.0% | |
| CGCTGTGCATTCTCTGGTGG+TGG | - | Chr3:76039758-76039777 | None:intergenic | 60.0% | |
| GACAGCAACAGCATCCGCAC+CGG | - | Chr3:76039811-76039830 | None:intergenic | 60.0% | |
| TCTCTGTAACGGCGTCCTGC+CGG | + | Chr3:76040086-76040105 | MsG0380015862.01.T01:CDS | 60.0% | |
| ! | AGCGGTGTTGGAAGGTGGAG+AGG | - | Chr3:76039675-76039694 | None:intergenic | 60.0% |
| ! | GATGGAGCGGTGTTGGAAGG+TGG | - | Chr3:76039680-76039699 | None:intergenic | 60.0% |
| !! | ATCCGCACCGGCGACTTTTG+CGG | - | Chr3:76039799-76039818 | None:intergenic | 60.0% |
| GTGCTCATGGCCATCGCTGC+CGG | + | Chr3:76039866-76039885 | MsG0380015862.01.T01:CDS | 65.0% | |
| TATGTTCCGGACGGCGGCGA+TGG | - | Chr3:76039698-76039717 | None:intergenic | 65.0% | |
| ! | GGCGATGGAGCGGTGTTGGA+AGG | - | Chr3:76039683-76039702 | None:intergenic | 65.0% |
| !! | CTCTGGTGGTGGGATGCGGT+AGG | - | Chr3:76039747-76039766 | None:intergenic | 65.0% |
| CGCCGCAAAAGTCGCCGGTG+CGG | + | Chr3:76039794-76039813 | MsG0380015862.01.T01:CDS | 70.0% | |
| ACCGCTCCATCGCCGCCGTC+CGG | + | Chr3:76039689-76039708 | MsG0380015862.01.T01:CDS | 75.0% | |
| CGGCGGCGATGGAGCGGTGT+TGG | - | Chr3:76039687-76039706 | None:intergenic | 75.0% | |
| TCCGGACGGCGGCGATGGAG+CGG | - | Chr3:76039693-76039712 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 76039647 | 76040135 | 76039647 | ID=MsG0380015862.01;Name=MsG0380015862.01 |
| Chr3 | mRNA | 76039647 | 76040135 | 76039647 | ID=MsG0380015862.01.T01;Parent=MsG0380015862.01;Name=MsG0380015862.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|162 |
| Chr3 | exon | 76039647 | 76040135 | 76039647 | ID=MsG0380015862.01.T01:exon:1197;Parent=MsG0380015862.01.T01 |
| Chr3 | CDS | 76039647 | 76040135 | 76039647 | ID=MsG0380015862.01.T01:cds;Parent=MsG0380015862.01.T01 |
| Gene Sequence |
| Protein sequence |
