AlfalfaGEDB | MsG0380016212.01

M. sativa cultivar ZhongmuNo.1 / MsG0380016212.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016212.01.T01	XP_024635983.1	82.338	1172	81	9	1	1086	45	1176	0	1731

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280007207.01	MsG0380016212.01	0.808394	3.165085e-50	1.807668e-47
MsG0380016212.01	MsG0580029511.01	-0.808576	2.893788e-50	1.660551e-47
MsG0380016212.01	MsG0680035239.01	0.805634	1.214123e-49	6.455917e-47
MsG0380016212.01	MsG0880041901.01	0.817956	2.520256e-52	1.861570e-49

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016212.01.T01	MTR_2g025250	85.714	56	8	0	989	1044	1	56	5.26e-25	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016212.01.T01	AT1G27850	36.394	1187	571	39	1	1086	45	1148	2.61e-146	468

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Find 281 sgRNAs with CRISPR-Local

Find 442 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTCCAGCAGCAACAA+AGG	0.548105	3:-80529543	MsG0380016212.01.T01:CDS
AAAAGTCGCAGAAGCAGTAG+GGG	0.605481	3:-80532225	MsG0380016212.01.T01:CDS
AAAATTAAAACCATATTAAC+GGG	0.417429	3:+80527790	None:intergenic
AAAGAAGTACGAAGATTGGG+AGG	0.653347	3:+80531863	None:intergenic
AAAGATGAACTGGGGCTTGG+AGG	0.459031	3:+80529294	None:intergenic
AAAGCCCTCAAAACATGTTC+AGG	0.414525	3:-80530530	MsG0380016212.01.T01:CDS
AAAGGTTCCATTGCATCATC+TGG	0.445401	3:-80531680	MsG0380016212.01.T01:CDS
AAAGTCCTGGCACGAACTAT+AGG	0.424404	3:+80529519	None:intergenic
AAATACAGAAACGTCAGTTA+AGG	0.488527	3:-80528914	MsG0380016212.01.T01:intron
AAATATGAACACGGACCCCA+TGG	0.597200	3:-80529592	MsG0380016212.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATACAGAAACGTCAGTTA+AGG	-	Chr3:80531669-80531688	MsG0380016212.01.T01:CDS	30.0%
AACATTAAACCAATTGGCAA+TGG	+	Chr3:80532845-80532864	None:intergenic	30.0%
AACCAACAGTAACTTTATTG+GGG	-	Chr3:80532480-80532499	MsG0380016212.01.T01:CDS	30.0%
AACTAATACATTGCAAGCAA+GGG	-	Chr3:80528423-80528442	MsG0380016212.01.T01:intron	30.0%
AACTGTTCTCTTATGCTTTA+TGG	-	Chr3:80532283-80532302	MsG0380016212.01.T01:intron	30.0%
AAGATCATCCAAATAATGCA+CGG	-	Chr3:80531638-80531657	MsG0380016212.01.T01:CDS	30.0%
AATCATGGTTAAAGGAATGA+TGG	+	Chr3:80529951-80529970	None:intergenic	30.0%
ACAGATTAACTATCCTTCTA+AGG	-	Chr3:80529574-80529593	MsG0380016212.01.T01:CDS	30.0%
AGAAGGATAGTTAATCTGTA+GGG	+	Chr3:80529573-80529592	None:intergenic	30.0%
AGTAGGCTATTATTAGTTAG+AGG	-	Chr3:80532954-80532973	MsG0380016212.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	80527504	80533101	80527504	ID=MsG0380016212.01;Name=MsG0380016212.01
Chr3	mRNA	80527504	80533101	80527504	ID=MsG0380016212.01.T01;Parent=MsG0380016212.01;Name=MsG0380016212.01.T01;_AED=0.24;_eAED=0.26;_QI=0\|0.57\|0.25\|1\|1\|1\|8\|362\|1086
Chr3	exon	80527504	80528296	80527504	ID=MsG0380016212.01.T01:exon:32843;Parent=MsG0380016212.01.T01
Chr3	exon	80528640	80528928	80528640	ID=MsG0380016212.01.T01:exon:32842;Parent=MsG0380016212.01.T01
Chr3	exon	80529017	80529752	80529017	ID=MsG0380016212.01.T01:exon:32841;Parent=MsG0380016212.01.T01
Chr3	exon	80529829	80530559	80529829	ID=MsG0380016212.01.T01:exon:32840;Parent=MsG0380016212.01.T01
Chr3	exon	80531502	80531754	80531502	ID=MsG0380016212.01.T01:exon:32839;Parent=MsG0380016212.01.T01
Chr3	exon	80531841	80532253	80531841	ID=MsG0380016212.01.T01:exon:32838;Parent=MsG0380016212.01.T01
Chr3	exon	80532397	80532517	80532397	ID=MsG0380016212.01.T01:exon:32837;Parent=MsG0380016212.01.T01
Chr3	exon	80532815	80533101	80532815	ID=MsG0380016212.01.T01:exon:32836;Parent=MsG0380016212.01.T01
Chr3	CDS	80532815	80533101	80532815	ID=MsG0380016212.01.T01:cds;Parent=MsG0380016212.01.T01
Chr3	CDS	80532397	80532517	80532397	ID=MsG0380016212.01.T01:cds;Parent=MsG0380016212.01.T01
Chr3	CDS	80531841	80532253	80531841	ID=MsG0380016212.01.T01:cds;Parent=MsG0380016212.01.T01
Chr3	CDS	80531502	80531754	80531502	ID=MsG0380016212.01.T01:cds;Parent=MsG0380016212.01.T01
Chr3	CDS	80529829	80530559	80529829	ID=MsG0380016212.01.T01:cds;Parent=MsG0380016212.01.T01
Chr3	CDS	80529017	80529752	80529017	ID=MsG0380016212.01.T01:cds;Parent=MsG0380016212.01.T01
Chr3	CDS	80528640	80528928	80528640	ID=MsG0380016212.01.T01:cds;Parent=MsG0380016212.01.T01
Chr3	CDS	80527866	80528296	80527866	ID=MsG0380016212.01.T01:cds;Parent=MsG0380016212.01.T01
Chr3	three_prime_UTR	80527504	80527865	80527504	ID=MsG0380016212.01.T01:three_prime_utr;Parent=MsG0380016212.01.T01

Gene Sequence

>MsG0380016212.01.T01

ATGCAGTCTAGAGAAAGAGATACTTTTTTGCTTCAGTCTTCTGATGACTTGGAAGATTCATTCGGTAATTTTCTTTGGTCATTTCTTATCGAAGATAAGGTGCCGTTCTTTGGTTTCTTCATTTGGGGTGATATTCATTCATATATTTCTTACTTTTATCTTTGGGTATTATTTTACAGCTACAAGGCTGAGACATTTTCTGAAGTAAACGTTGGAATCTCCATTCCTGGTCGGCGATTAAACAGTGACTTGCTCAATGTAGATGGTGACAAGAATGACTATGACTGGTTATTGACACCCCCGGATACGCCACTGTTTCCTTCATTAGATGAAGATCCACCACCTACTAATGTTGCAAGCAGAGGAAGACCTCAGAGTAAACCCATTACTATATCAAGATCTTCTACGATGGAAAAAAGTCGCAGAAGCAGTAGGGGCAGTGCAAGTCCAAATCGTTTAAGTCCATCTCCTCGTTCGGGAACTAATACATTGCAAGCAAGGGGAAGGCCTTCATCAGTGCCAAATTACAGTCCAACCTCCTCAACTTTACGAAATGCTACTCCAACAAGAAGACCATCACCACCTCCAAATAAGCCCACGACACCTGCTTCTAAGTCCTCCACTTATACTCCACGGAGATTGAGCACTGGTTCCAGTGGCTCTGTTGCCTCATCTGGAGTTAGGGGAACTTCCCCAGTTAAGACAAGTCGTGGAAACTCTTCATCACCAAAGATAAGGGCATGGCAAACTAATATACCGGCTTCTCTTCTGAAGCTCCTCCCAATCTTCGTACTTCTTTGGCTGATCGACCAGCAACGTATGAGTAGTAGCTCTATTCAAAGTCACGATCGAGACCCATTCAGCTCACGCAGCAAAGGTTCCATTGCATCATCTGGTGATGATGATCTTGACTCTCTTCAGTCCATCCCAGTGGGTAGCTTAGACAGACTGAGTTCAAGAAGGGATGGTTCATTTTCAATCAACAGAACTCCTGCCATGTCCAGGAAATCACCCAGGACGGCGTCCCCAAATTCTGCTCCTAAAAAATCATTCGACTCTGCTTTCCGGCAAATGGATAGGAAAAGCCCTCAAAACATGTTCAGGCCACTTTTATCAAGTGCTCCAAGTACAACCCTTTATGCTGGAAATTCAAATTCTGCACATCGTTCCCTTGTATATAGGAACTCCTCCATTGCAACAAGCGGCAATGCAACCTCCGATCGAGTTACAGCTATTGCTCTAGATACTGAAGGAATTGACCATAATCAAGACGATACGGCAAGTGAAACTGATAAGATGTTATATCCTGATTTAGATGAAGAAGTATTTGCCTTTGATAAGATGGATGAATTAAATGCAAATATTAAGCATGAAATCGACGAGCAATCAGTCAATATCCTGCAAAATCAAAGCAGAGGCCCCAATACAGTTTTTGGTCCAACTGAAGCTGAGGGTTCTGTTTACCATGCTCATATTGACGCCGAAGGCAATGAAAGTTCAGAAACATCACATGTCAGAGATGTCATTTCTGAAATTGGTAGTTTTGAAAATACAGCAATCTGTTCTCAATGTGGTTGTTGTTATCAAGTCATTAATCAAACTGAGGAGAATATTGAACTTTGTCCCCAATGCAGCAGGAAAGCCACATTGTTGAGAGCCATCCTTCCTGAGACAATGTTGGCTGTATCTGAAGGTTCTTCAATGATTTCTGCGAACATGCCCAAAGAAGAAAAATCATTAGCTGAAACAAGCCAACTACGGGCTGCATCTGAACTTCCTCAAGAGACTGATACGGATAACTTGAGTTCAATTCCAAGTTCATTGAGGGATGGAGGTAAGAAGTTACCTACAAACCAGCTAGAGATGAACCAATCAGAAGTTGATTACAAGAAGCACAACGATGAATTCGAGGATCAGCCATTGGATCACTACAGTGATCGCCCAAATATGAACACGGACCCCATGGTCGGCACCGGCATTTCTGTATTGCTGAAAAGGTCCAGCAGCAACAAAGGACCTATAGTTCGTGCCAGGACTTTTACTGCCACAACCATATCTTACGATGATCTGTCTCTTTCAAGAGACAGTGTAAACAGTGTTAGAAGTTCAACGAGACCTGGCAGTTATTCTGCCTCATCATCGACTGATTTCAGCTCTACTAGACAATCAGAGTTTCGTATACACAGGCAGTTGAGTGGCAGGAAATTGGATGCAGACTGCGGATATGACTTAAGGATTAAGCCTCCAAGCCCCAGTTCATCTTTGTCTATAACTTCAAGCCATTCTCACCAGGAAGTAGGCCTTGCTACTCGAGAAGCTTCTGGTAACGCAGAATGTAGCCTTGTGGAGGAGATACCTCAAGTTCTGCAAGAAACTCAAGCTTCAGGAAATGCAATGACAGATGTAGTTGGTGCTTCTCCTATCGATTCAACTTATGTTGAAGAAGATAAACTAGAAAATGGTGACAGTAGTAGAGGAAACAATGCTTGCTGCTCAGAATTTTTAAGTCAGGAAGCTGTTTTTCAGCCTGATGAAAACTTAAAACGTCAGTTAAGGCTCCAGAGTTATCATGTCACGAGAAGCATGATGTGGAGTTCTAATGCTAATGAACTGAATGTTTCGGTTATTGCCAACTGTTCTACAATCACAGAATCAGAAATAGAAGGGGAAAACAATTGTGGGAATAATATCAACCTGGTGAATGATGATATGTCCCTGGTATCAAAGAGTGCCCTCGATGACTTCCAGGAATCTTCTGCTCGAAATCCTTCCAATGATTGCCACACTGCTTCTGTTTCAGAGGTCAATTTATCTGAGTCTCATGGCACTGAGGAATCAACAGTTACAGTGGAGTGTCAAGGTGCAGGCAACACTAGAAGCCTGACACTTGAAGAGGCAACAGATACAATACTTTTTTGCAGTTCTATCATCCATGATCTAGCATATAAAGCTGCAACAATAGCAATGGAAAATGAATCTTCCGATCCATTCGAAGGTTCTGAACCAACAGTAACTTTATTGGGGAAACCTGTTTCTGACAGAAAGGATGTTCGCAGACGTCCCGTGGGTAAGCGCACTATAAAAACTCCGAAGACCAGACAGAAGAGTGTGGAAATGGACGTCAAAACCGTTTCTGGCAAGACTGAGAATGATGAAAATATTGACGAGTCTTTCACAAATAATGTTGGGCTTCCTAACAAGTTAGATAATAGTATGAAGCCCCCAAAGCTTGAATCAAAGTGCAATTGCATCATAATGTGA

Protein sequence

>MsG0380016212.01.T01

MQSRERDTFLLQSSDDLEDSFGNFLWSFLIEDKVPFFGFFIWGDIHSYISYFYLWVLFYSYKAETFSEVNVGISIPGRRLNSDLLNVDGDKNDYDWLLTPPDTPLFPSLDEDPPPTNVASRGRPQSKPITISRSSTMEKSRRSSRGSASPNRLSPSPRSGTNTLQARGRPSSVPNYSPTSSTLRNATPTRRPSPPPNKPTTPASKSSTYTPRRLSTGSSGSVASSGVRGTSPVKTSRGNSSSPKIRAWQTNIPASLLKLLPIFVLLWLIDQQRMSSSSIQSHDRDPFSSRSKGSIASSGDDDLDSLQSIPVGSLDRLSSRRDGSFSINRTPAMSRKSPRTASPNSAPKKSFDSAFRQMDRKSPQNMFRPLLSSAPSTTLYAGNSNSAHRSLVYRNSSIATSGNATSDRVTAIALDTEGIDHNQDDTASETDKMLYPDLDEEVFAFDKMDELNANIKHEIDEQSVNILQNQSRGPNTVFGPTEAEGSVYHAHIDAEGNESSETSHVRDVISEIGSFENTAICSQCGCCYQVINQTEENIELCPQCSRKATLLRAILPETMLAVSEGSSMISANMPKEEKSLAETSQLRAASELPQETDTDNLSSIPSSLRDGGKKLPTNQLEMNQSEVDYKKHNDEFEDQPLDHYSDRPNMNTDPMVGTGISVLLKRSSSNKGPIVRARTFTATTISYDDLSLSRDSVNSVRSSTRPGSYSASSSTDFSSTRQSEFRIHRQLSGRKLDADCGYDLRIKPPSPSSSLSITSSHSHQEVGLATREASGNAECSLVEEIPQVLQETQASGNAMTDVVGASPIDSTYVEEDKLENGDSSRGNNACCSEFLSQEAVFQPDENLKRQLRLQSYHVTRSMMWSSNANELNVSVIANCSTITESEIEGENNCGNNINLVNDDMSLVSKSALDDFQESSARNPSNDCHTASVSEVNLSESHGTEESTVTVECQGAGNTRSLTLEEATDTILFCSSIIHDLAYKAATIAMENESSDPFEGSEPTVTLLGKPVSDRKDVRRRPVGKRTIKTPKTRQKSVEMDVKTVSGKTENDENIDESFTNNVGLPNKLDNSMKPPKLESKCNCIIM*