AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016321.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016321.01.T01 MTR_3g087770 99.342 152 1 0 1 152 1 152 3.06e-112 315
MsG0380016321.01.T01 MTR_8g091420 81.935 155 23 3 1 152 1 153 1.35e-79 232
MsG0380016321.01.T01 MTR_1g030630 56.376 149 61 3 5 152 2 147 6.26e-56 172
MsG0380016321.01.T01 MTR_3g073670 59.091 154 56 3 1 152 1 149 1.64e-55 171
MsG0380016321.01.T01 MTR_5g022620 55.769 156 61 4 5 152 2 157 2.01e-53 166
MsG0380016321.01.T01 MTR_3g117890 53.750 160 57 5 5 152 2 156 2.41e-52 163
MsG0380016321.01.T01 MTR_4g073040 54.194 155 64 4 5 152 2 156 7.42e-52 162
MsG0380016321.01.T01 MTR_6g003990 50.649 154 70 3 5 152 2 155 2.73e-51 160
MsG0380016321.01.T01 MTR_5g025150 52.027 148 67 1 5 152 2 145 6.57e-51 159
MsG0380016321.01.T01 MTR_7g083060 57.364 129 53 2 25 152 19 146 2.51e-47 150
MsG0380016321.01.T01 MTR_6g003990 53.390 118 52 2 38 152 1 118 3.29e-42 136
MsG0380016321.01.T01 MTR_5g055020 47.967 123 64 0 30 152 25 147 2.56e-40 132
MsG0380016321.01.T01 MTR_6g026890 39.456 147 77 4 17 152 3 148 2.87e-28 102
MsG0380016321.01.T01 MTR_1g078090 41.045 134 69 3 29 152 52 185 1.27e-27 101
MsG0380016321.01.T01 MTR_2g091235 35.135 148 66 5 29 152 1 142 6.38e-22 85.9
MsG0380016321.01.T01 MTR_6g045203 39.706 136 67 4 32 152 5 140 3.87e-18 75.9
MsG0380016321.01.T01 MTR_1g082650 53.731 67 31 0 28 94 8 74 4.92e-15 71.6
MsG0380016321.01.T01 MTR_1g107565 50.746 67 33 0 28 94 11 77 8.11e-15 70.5
MsG0380016321.01.T01 MTR_2g022250 47.761 67 35 0 28 94 8 74 7.51e-14 68.2
MsG0380016321.01.T01 MTR_1g092670 49.231 65 33 0 30 94 4 68 1.09e-13 62.8
MsG0380016321.01.T01 MTR_7g013660 47.692 65 34 0 30 94 4 68 1.25e-13 63.9
MsG0380016321.01.T01 MTR_7g108560 49.231 65 33 0 30 94 22 86 1.35e-13 66.6
MsG0380016321.01.T01 MTR_4g094238 38.824 85 49 2 29 113 1 82 1.47e-13 62.8
MsG0380016321.01.T01 MTR_4g117140 46.269 67 36 0 28 94 8 74 3.11e-13 66.2
MsG0380016321.01.T01 MTR_4g117220 46.269 67 36 0 28 94 8 74 3.37e-13 66.2
MsG0380016321.01.T01 MTR_7g113110 48.387 62 32 0 34 95 37 98 1.14e-12 64.3
MsG0380016321.01.T01 MTR_3g067750 46.875 64 34 0 30 93 12 75 5.22e-12 61.2
MsG0380016321.01.T01 MTR_4g119820 46.970 66 35 0 30 95 74 139 1.46e-11 61.2
MsG0380016321.01.T01 MTR_1g083310 44.776 67 36 1 30 95 15 81 2.91e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016321.01.T01 AT4G39700 70.886 158 40 3 1 152 1 158 3.77e-72 214
MsG0380016321.01.T01 AT1G22990 56.579 152 61 3 5 152 2 152 8.38e-58 177
MsG0380016321.01.T01 AT1G71050 59.355 155 57 3 1 152 1 152 7.60e-56 172
MsG0380016321.01.T01 AT1G22990 55.034 149 63 3 5 152 2 147 7.52e-55 169
MsG0380016321.01.T01 AT4G38580 54.902 153 63 3 5 152 2 153 1.28e-54 169
MsG0380016321.01.T01 AT4G38580 54.902 153 63 3 5 152 2 153 1.28e-54 169
MsG0380016321.01.T01 AT4G08570 55.844 154 62 3 1 152 1 150 5.08e-54 167
MsG0380016321.01.T01 AT5G17450 50.667 150 70 4 5 152 2 149 5.94e-46 147
MsG0380016321.01.T01 AT5G66110 51.007 149 63 4 10 152 3 147 9.15e-45 144
MsG0380016321.01.T01 AT5G66110 51.007 149 63 4 10 152 3 147 9.15e-45 144
MsG0380016321.01.T01 AT4G35060 47.403 154 73 4 5 152 2 153 2.09e-43 140
MsG0380016321.01.T01 AT5G66110 52.893 121 51 3 38 152 1 121 5.49e-38 126
MsG0380016321.01.T01 AT5G17450 55.556 99 41 3 56 152 8 105 3.42e-31 108
MsG0380016321.01.T01 AT1G06330 38.961 154 60 5 29 152 1 150 1.33e-26 98.2
MsG0380016321.01.T01 AT3G56891 39.216 153 63 6 29 152 15 166 6.44e-25 94.4
MsG0380016321.01.T01 AT2G18196 36.301 146 81 5 19 152 33 178 2.77e-24 92.8
MsG0380016321.01.T01 AT4G10465 38.849 139 75 3 24 152 45 183 1.91e-23 90.9
MsG0380016321.01.T01 AT3G56891 38.194 144 59 6 38 152 1 143 2.54e-22 87.0
MsG0380016321.01.T01 AT1G29100 37.584 149 61 4 31 152 4 147 3.14e-21 84.3
MsG0380016321.01.T01 AT1G29100 36.986 146 60 4 34 152 1 141 5.67e-20 80.9
MsG0380016321.01.T01 AT2G18196 35.200 125 71 4 38 152 1 125 4.32e-19 78.2
MsG0380016321.01.T01 AT3G48970 38.686 137 69 4 31 152 4 140 7.70e-15 67.4
MsG0380016321.01.T01 AT3G48970 38.686 137 69 4 31 152 2 138 9.21e-15 67.4
MsG0380016321.01.T01 AT3G48970 38.686 137 69 4 31 152 2 138 9.21e-15 67.4
MsG0380016321.01.T01 AT1G66240 44.444 72 40 0 23 94 26 97 7.62e-14 63.9
MsG0380016321.01.T01 AT5G27690 48.387 62 32 0 34 95 34 95 1.52e-13 67.0
MsG0380016321.01.T01 AT3G06130 48.485 66 34 0 29 94 9 74 2.12e-13 66.6
MsG0380016321.01.T01 AT3G56240 50.000 66 33 0 30 95 3 68 2.89e-13 62.8
MsG0380016321.01.T01 AT3G56240 50.000 66 33 0 30 95 3 68 2.89e-13 62.8
MsG0380016321.01.T01 AT3G06130 47.761 67 35 0 28 94 8 74 4.00e-13 65.9
MsG0380016321.01.T01 AT5G19090 46.269 67 36 0 28 94 8 74 3.66e-12 63.2
MsG0380016321.01.T01 AT5G19090 46.269 67 36 0 28 94 8 74 3.66e-12 63.2
MsG0380016321.01.T01 AT5G19090 46.269 67 36 0 28 94 8 74 3.66e-12 63.2
MsG0380016321.01.T01 AT5G19090 46.269 67 36 0 28 94 8 74 4.65e-12 62.8
MsG0380016321.01.T01 AT5G19090 46.269 67 36 0 28 94 8 74 4.65e-12 62.8
MsG0380016321.01.T01 AT1G56210 49.231 65 33 0 34 98 44 108 5.63e-12 62.4
MsG0380016321.01.T01 AT1G23000 42.857 70 40 0 29 98 72 141 1.84e-11 61.2
MsG0380016321.01.T01 AT1G23000 42.857 70 40 0 29 98 12 81 2.02e-11 60.8
MsG0380016321.01.T01 AT5G37860 40.845 71 42 0 29 99 9 79 3.78e-11 59.7

Find 41 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTATCATCATCTACATAA+TGG 0.292404 3:+81959199 None:intergenic
TACATAATGGAGCAGGCATT+TGG 0.304332 3:+81959212 None:intergenic
AGAGTACTTATCTGATCTAA+TGG 0.306737 3:-81959904 MsG0380016321.01.T01:CDS
TAAAATCAGTGGAGATAAAC+AGG 0.372946 3:-81959478 MsG0380016321.01.T01:CDS
TTGCGATGGTTGCGAACTTA+AGG 0.383976 3:-81959808 MsG0380016321.01.T01:CDS
ACCATGTTGTATGGCACATA+AGG 0.433890 3:+81959365 None:intergenic
AGCAAAGTCAACAGGAAAGA+AGG 0.439458 3:-81959399 MsG0380016321.01.T01:CDS
CAGGAAAGAAGGCAGAGATT+TGG 0.440709 3:-81959388 MsG0380016321.01.T01:CDS
ACATAATGGAGCAGGCATTT+GGG 0.448683 3:+81959213 None:intergenic
GGGGTATGTAGAGGCAAATA+AGG 0.452605 3:-81959432 MsG0380016321.01.T01:CDS
TCCTGGATATGTAAGGAGAT+TGG 0.456785 3:-81959300 MsG0380016321.01.T01:CDS
TCCAATCTCCTTACATATCC+AGG 0.463407 3:+81959299 None:intergenic
CTAAAGAAAGCAAAGTCAAC+AGG 0.471841 3:-81959407 MsG0380016321.01.T01:CDS
GAGTACTTATCTGATCTAAT+GGG 0.482086 3:-81959903 MsG0380016321.01.T01:CDS
AAAGCCCTCTCTTCAATGAA+TGG 0.498175 3:-81959780 MsG0380016321.01.T01:intron
ATTACAGACCGTAGAGTTGA+AGG 0.499504 3:-81959841 MsG0380016321.01.T01:CDS
TATGACAAGAAGGCTCCTCC+TGG 0.508900 3:-81959317 MsG0380016321.01.T01:CDS
CGTAGAGTTGAAGGTGAGGA+TGG 0.509056 3:-81959832 MsG0380016321.01.T01:CDS
CTTACCATTCATTGAAGAGA+GGG 0.529075 3:+81959776 None:intergenic
ATATACACAGAAATGGGAGT+TGG 0.529253 3:-81959936 None:intergenic
AGGCTCCTCCTGGATATGTA+AGG 0.531905 3:-81959307 MsG0380016321.01.T01:CDS
GGAGCCTTCTTGTCATAAGA+AGG 0.533318 3:+81959323 None:intergenic
GGATGAGCCACCATGTTGTA+TGG 0.533759 3:+81959356 None:intergenic
TCATAAGAAGGAGCAGCGTA+TGG 0.549598 3:+81959335 None:intergenic
TACACAGAAATGGGAGTTGG+AGG 0.552311 3:-81959933 None:intergenic
CATCATCTACATAATGGAGC+AGG 0.555456 3:+81959205 None:intergenic
TGCTCCTTCTTATGACAAGA+AGG 0.562724 3:-81959327 MsG0380016321.01.T01:CDS
GTAAGGAGATTGGAGACCAC+TGG 0.572647 3:-81959290 MsG0380016321.01.T01:CDS
TTATCTGATCTAATGGGCAG+TGG 0.573748 3:-81959897 MsG0380016321.01.T01:CDS
CATCCTCACCTTCAACTCTA+CGG 0.585179 3:+81959833 None:intergenic
GACAGTAACGGGGTATGTAG+AGG 0.602619 3:-81959441 MsG0380016321.01.T01:CDS
ACTTACCATTCATTGAAGAG+AGG 0.616934 3:+81959775 None:intergenic
CATCACTAAACATGGTGGTG+AGG 0.624282 3:+81959240 None:intergenic
GCCTTATGTGCCATACAACA+TGG 0.647674 3:-81959366 MsG0380016321.01.T01:CDS
ATATGCTCTAACAGTTCCAG+TGG 0.649114 3:+81959274 None:intergenic
AAGGTGAGGATGGATTGCGA+TGG 0.658836 3:-81959822 MsG0380016321.01.T01:CDS
CTAAACATGGTGGTGAGGTG+TGG 0.665051 3:+81959245 None:intergenic
AGACCGTAGAGTTGAAGGTG+AGG 0.688480 3:-81959836 MsG0380016321.01.T01:CDS
TGATGCAGGAGTAAAATCAG+TGG 0.694945 3:-81959489 MsG0380016321.01.T01:intron
TTATGTGCCATACAACATGG+TGG 0.751150 3:-81959363 MsG0380016321.01.T01:CDS
AATCTCCTTACATATCCAGG+AGG 0.766746 3:+81959302 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATTTTTTTTTTTTTTTG+TGG - Chr3:81959623-81959642 MsG0380016321.01.T01:intron 10.0%
!! AGAGAAAGATAAATTAATAG+AGG + Chr3:81959395-81959414 None:intergenic 20.0%
!! GAGAAAGATAAATTAATAGA+GGG + Chr3:81959394-81959413 None:intergenic 20.0%
! TCAAATTATAATTAGTGCGT+CGG - Chr3:81959505-81959524 MsG0380016321.01.T01:intron 25.0%
ACAACAAAAAGTGACAGTAA+CGG - Chr3:81959682-81959701 MsG0380016321.01.T01:intron 30.0%
GAGTACTTATCTGATCTAAT+GGG - Chr3:81959232-81959251 MsG0380016321.01.T01:CDS 30.0%
TAAAATCAGTGGAGATAAAC+AGG - Chr3:81959657-81959676 MsG0380016321.01.T01:intron 30.0%
TATCTTTCTCTATATAGCAC+CGG - Chr3:81959404-81959423 MsG0380016321.01.T01:CDS 30.0%
! AGAGTACTTATCTGATCTAA+TGG - Chr3:81959231-81959250 MsG0380016321.01.T01:CDS 30.0%
! TCTTCTTCTTGATTTTGTGA+TGG + Chr3:81959270-81959289 None:intergenic 30.0%
!!! TTTTTTTTTTGTGGTGATGC+AGG - Chr3:81959632-81959651 MsG0380016321.01.T01:intron 30.0%
AACAAAAAGTGACAGTAACG+GGG - Chr3:81959684-81959703 MsG0380016321.01.T01:intron 35.0%
ACTTACCATTCATTGAAGAG+AGG + Chr3:81959363-81959382 None:intergenic 35.0%
CAACAAAAAGTGACAGTAAC+GGG - Chr3:81959683-81959702 MsG0380016321.01.T01:intron 35.0%
CTAAAGAAAGCAAAGTCAAC+AGG - Chr3:81959728-81959747 MsG0380016321.01.T01:intron 35.0%
CTTACCATTCATTGAAGAGA+GGG + Chr3:81959362-81959381 None:intergenic 35.0%
GCTTGCTTGCATTATATGTA+TGG - Chr3:81959561-81959580 MsG0380016321.01.T01:intron 35.0%
! GTTTTCATCACTAAACATGG+TGG + Chr3:81959903-81959922 None:intergenic 35.0%
! TCTTCTTGATTTTGTGATGG+TGG + Chr3:81959267-81959286 None:intergenic 35.0%
! TGGGTTTTCATCACTAAACA+TGG + Chr3:81959906-81959925 None:intergenic 35.0%
AAAGCCCTCTCTTCAATGAA+TGG - Chr3:81959355-81959374 MsG0380016321.01.T01:CDS 40.0%
AATCTCCTTACATATCCAGG+AGG + Chr3:81959836-81959855 None:intergenic 40.0%
ACCATGTTGTATGGCACATA+AGG + Chr3:81959773-81959792 None:intergenic 40.0%
ACGTCATCAATATGAAGTGC+CGG + Chr3:81959426-81959445 None:intergenic 40.0%
AGCAAAGTCAACAGGAAAGA+AGG - Chr3:81959736-81959755 MsG0380016321.01.T01:intron 40.0%
ATATGCTCTAACAGTTCCAG+TGG + Chr3:81959864-81959883 None:intergenic 40.0%
ATTACAGACCGTAGAGTTGA+AGG - Chr3:81959294-81959313 MsG0380016321.01.T01:CDS 40.0%
TCCAATCTCCTTACATATCC+AGG + Chr3:81959839-81959858 None:intergenic 40.0%
TCCTGGATATGTAAGGAGAT+TGG - Chr3:81959835-81959854 MsG0380016321.01.T01:CDS 40.0%
TGATGCAGGAGTAAAATCAG+TGG - Chr3:81959646-81959665 MsG0380016321.01.T01:intron 40.0%
TGCTCCTTCTTATGACAAGA+AGG - Chr3:81959808-81959827 MsG0380016321.01.T01:CDS 40.0%
TTATGTGCCATACAACATGG+TGG - Chr3:81959772-81959791 MsG0380016321.01.T01:intron 40.0%
! ACATAATGGAGCAGGCATTT+GGG + Chr3:81959925-81959944 None:intergenic 40.0%
! TACATAATGGAGCAGGCATT+TGG + Chr3:81959926-81959945 None:intergenic 40.0%
! TTATCTGATCTAATGGGCAG+TGG - Chr3:81959238-81959257 MsG0380016321.01.T01:CDS 40.0%
CAGGAAAGAAGGCAGAGATT+TGG - Chr3:81959747-81959766 MsG0380016321.01.T01:intron 45.0%
CATCCTCACCTTCAACTCTA+CGG + Chr3:81959305-81959324 None:intergenic 45.0%
GCCTTATGTGCCATACAACA+TGG - Chr3:81959769-81959788 MsG0380016321.01.T01:intron 45.0%
GGAGCCTTCTTGTCATAAGA+AGG + Chr3:81959815-81959834 None:intergenic 45.0%
GGGGTATGTAGAGGCAAATA+AGG - Chr3:81959703-81959722 MsG0380016321.01.T01:intron 45.0%
TCATAAGAAGGAGCAGCGTA+TGG + Chr3:81959803-81959822 None:intergenic 45.0%
TTGCGATGGTTGCGAACTTA+AGG - Chr3:81959327-81959346 MsG0380016321.01.T01:CDS 45.0%
! CATCACTAAACATGGTGGTG+AGG + Chr3:81959898-81959917 None:intergenic 45.0%
AGACCGTAGAGTTGAAGGTG+AGG - Chr3:81959299-81959318 MsG0380016321.01.T01:CDS 50.0%
AGGCTCCTCCTGGATATGTA+AGG - Chr3:81959828-81959847 MsG0380016321.01.T01:CDS 50.0%
CGTAGAGTTGAAGGTGAGGA+TGG - Chr3:81959303-81959322 MsG0380016321.01.T01:CDS 50.0%
CGTCTGACATCACTGACACA+TGG - Chr3:81959452-81959471 MsG0380016321.01.T01:CDS 50.0%
GGATGAGCCACCATGTTGTA+TGG + Chr3:81959782-81959801 None:intergenic 50.0%
GTAAGGAGATTGGAGACCAC+TGG - Chr3:81959845-81959864 MsG0380016321.01.T01:CDS 50.0%
GTGTCAGTGATGTCAGACGT+TGG + Chr3:81959451-81959470 None:intergenic 50.0%
TATGACAAGAAGGCTCCTCC+TGG - Chr3:81959818-81959837 MsG0380016321.01.T01:CDS 50.0%
! CTAAACATGGTGGTGAGGTG+TGG + Chr3:81959893-81959912 None:intergenic 50.0%
! GACAGTAACGGGGTATGTAG+AGG - Chr3:81959694-81959713 MsG0380016321.01.T01:intron 50.0%
!! AAGGTGAGGATGGATTGCGA+TGG - Chr3:81959313-81959332 MsG0380016321.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 81959211 81959946 81959211 ID=MsG0380016321.01;Name=MsG0380016321.01
Chr3 mRNA 81959211 81959946 81959211 ID=MsG0380016321.01.T01;Parent=MsG0380016321.01;Name=MsG0380016321.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|152
Chr3 exon 81959781 81959946 81959781 ID=MsG0380016321.01.T01:exon:11670;Parent=MsG0380016321.01.T01
Chr3 exon 81959211 81959503 81959211 ID=MsG0380016321.01.T01:exon:11669;Parent=MsG0380016321.01.T01
Chr3 CDS 81959781 81959946 81959781 ID=MsG0380016321.01.T01:cds;Parent=MsG0380016321.01.T01
Chr3 CDS 81959211 81959503 81959211 ID=MsG0380016321.01.T01:cds;Parent=MsG0380016321.01.T01
Gene Sequence

>MsG0380016321.01.T01

ATGGGAGTTGGAGGCACATTAGAGTACTTATCTGATCTAATGGGCAGTGGTCACCACCATCACAAAATCAAGAAGAAGAAGCAATTACAGACCGTAGAGTTGAAGGTGAGGATGGATTGCGATGGTTGCGAACTTAAGGTCAAGAAAGCCCTCTCTTCAATGAATGGAGTAAAATCAGTGGAGATAAACAGGAAACAACAAAAAGTGACAGTAACGGGGTATGTAGAGGCAAATAAGGTGCTAAAGAAAGCAAAGTCAACAGGAAAGAAGGCAGAGATTTGGCCTTATGTGCCATACAACATGGTGGCTCATCCATACGCTGCTCCTTCTTATGACAAGAAGGCTCCTCCTGGATATGTAAGGAGATTGGAGACCACTGGAACTGTTAGAGCATATGAAGAGCCACACCTCACCACCATGTTTAGTGATGAAAACCCAAATGCCTGCTCCATTATGTAG

Protein sequence

>MsG0380016321.01.T01

MGVGGTLEYLSDLMGSGHHHHKIKKKKQLQTVELKVRMDCDGCELKVKKALSSMNGVKSVEINRKQQKVTVTGYVEANKVLKKAKSTGKKAEIWPYVPYNMVAHPYAAPSYDKKAPPGYVRRLETTGTVRAYEEPHLTTMFSDENPNACSIM*