AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016326.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016326.01.T01 MTR_3g087770 98.684 152 2 0 1 152 1 152 2.87e-111 312
MsG0380016326.01.T01 MTR_8g091420 81.935 155 23 3 1 152 1 153 1.80e-79 232
MsG0380016326.01.T01 MTR_1g030630 56.376 149 61 3 5 152 2 147 6.19e-56 172
MsG0380016326.01.T01 MTR_3g073670 59.091 154 56 3 1 152 1 149 1.60e-55 171
MsG0380016326.01.T01 MTR_5g022620 55.128 156 62 4 5 152 2 157 7.97e-53 165
MsG0380016326.01.T01 MTR_3g117890 53.750 160 57 5 5 152 2 156 1.66e-52 164
MsG0380016326.01.T01 MTR_4g073040 54.194 155 64 4 5 152 2 156 6.23e-52 162
MsG0380016326.01.T01 MTR_6g003990 50.649 154 70 3 5 152 2 155 3.21e-51 160
MsG0380016326.01.T01 MTR_5g025150 52.027 148 67 1 5 152 2 145 6.43e-51 159
MsG0380016326.01.T01 MTR_7g083060 57.364 129 53 2 25 152 19 146 2.20e-47 150
MsG0380016326.01.T01 MTR_6g003990 53.390 118 52 2 38 152 1 118 3.59e-42 136
MsG0380016326.01.T01 MTR_5g055020 44.000 150 78 2 5 152 2 147 1.50e-40 133
MsG0380016326.01.T01 MTR_6g026890 39.456 147 77 4 17 152 3 148 2.21e-28 102
MsG0380016326.01.T01 MTR_1g078090 41.045 134 69 3 29 152 52 185 9.60e-28 102
MsG0380016326.01.T01 MTR_2g091235 35.135 148 66 5 29 152 1 142 4.98e-22 86.3
MsG0380016326.01.T01 MTR_6g045203 39.706 136 67 4 32 152 5 140 3.55e-18 76.3
MsG0380016326.01.T01 MTR_1g082650 53.731 67 31 0 28 94 8 74 4.43e-15 71.6
MsG0380016326.01.T01 MTR_1g107565 50.746 67 33 0 28 94 11 77 8.61e-15 70.5
MsG0380016326.01.T01 MTR_2g022250 47.761 67 35 0 28 94 8 74 7.08e-14 68.2
MsG0380016326.01.T01 MTR_1g092670 49.231 65 33 0 30 94 4 68 1.08e-13 62.8
MsG0380016326.01.T01 MTR_4g094238 38.824 85 49 2 29 113 1 82 1.29e-13 63.2
MsG0380016326.01.T01 MTR_7g013660 47.692 65 34 0 30 94 4 68 1.32e-13 63.9
MsG0380016326.01.T01 MTR_7g108560 49.231 65 33 0 30 94 22 86 1.34e-13 66.6
MsG0380016326.01.T01 MTR_4g117140 46.269 67 36 0 28 94 8 74 2.64e-13 66.6
MsG0380016326.01.T01 MTR_4g117220 46.269 67 36 0 28 94 8 74 2.92e-13 66.2
MsG0380016326.01.T01 MTR_7g113110 48.387 62 32 0 34 95 37 98 1.26e-12 64.3
MsG0380016326.01.T01 MTR_3g067750 46.875 64 34 0 30 93 12 75 5.01e-12 61.6
MsG0380016326.01.T01 MTR_4g119820 46.970 66 35 0 30 95 74 139 1.35e-11 61.2
MsG0380016326.01.T01 MTR_1g083310 44.776 67 36 1 30 95 15 81 2.45e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016326.01.T01 AT4G39700 70.886 158 40 3 1 152 1 158 4.50e-72 213
MsG0380016326.01.T01 AT1G22990 56.579 152 61 3 5 152 2 152 6.59e-58 177
MsG0380016326.01.T01 AT1G71050 59.355 155 57 3 1 152 1 152 6.45e-56 172
MsG0380016326.01.T01 AT1G22990 55.034 149 63 3 5 152 2 147 5.97e-55 170
MsG0380016326.01.T01 AT4G38580 54.902 153 63 3 5 152 2 153 1.13e-54 169
MsG0380016326.01.T01 AT4G38580 54.902 153 63 3 5 152 2 153 1.13e-54 169
MsG0380016326.01.T01 AT4G08570 55.844 154 62 3 1 152 1 150 5.19e-54 167
MsG0380016326.01.T01 AT5G17450 50.667 150 70 4 5 152 2 149 3.60e-46 147
MsG0380016326.01.T01 AT5G66110 51.007 149 63 4 10 152 3 147 7.27e-45 144
MsG0380016326.01.T01 AT5G66110 51.007 149 63 4 10 152 3 147 7.27e-45 144
MsG0380016326.01.T01 AT4G35060 47.403 154 73 4 5 152 2 153 1.34e-43 141
MsG0380016326.01.T01 AT5G66110 52.893 121 51 3 38 152 1 121 5.49e-38 126
MsG0380016326.01.T01 AT5G17450 55.556 99 41 3 56 152 8 105 2.72e-31 108
MsG0380016326.01.T01 AT1G06330 38.961 154 60 5 29 152 1 150 1.12e-26 98.2
MsG0380016326.01.T01 AT3G56891 39.216 153 63 6 29 152 15 166 6.58e-25 94.4
MsG0380016326.01.T01 AT2G18196 35.862 145 83 4 18 152 34 178 1.57e-24 93.6
MsG0380016326.01.T01 AT4G10465 38.849 139 75 3 24 152 45 183 1.79e-23 90.9
MsG0380016326.01.T01 AT3G56891 38.194 144 59 6 38 152 1 143 2.52e-22 87.0
MsG0380016326.01.T01 AT1G29100 37.584 149 61 4 31 152 4 147 2.85e-21 84.3
MsG0380016326.01.T01 AT1G29100 36.986 146 60 4 34 152 1 141 5.55e-20 80.9
MsG0380016326.01.T01 AT2G18196 35.200 125 71 4 38 152 1 125 3.33e-19 78.6
MsG0380016326.01.T01 AT3G48970 38.686 137 69 4 31 152 2 138 7.36e-15 67.4
MsG0380016326.01.T01 AT3G48970 38.686 137 69 4 31 152 2 138 7.36e-15 67.4
MsG0380016326.01.T01 AT3G48970 38.686 137 69 4 31 152 4 140 7.87e-15 67.4
MsG0380016326.01.T01 AT1G66240 44.444 72 40 0 23 94 26 97 7.62e-14 63.9
MsG0380016326.01.T01 AT5G27690 48.387 62 32 0 34 95 34 95 1.42e-13 67.0
MsG0380016326.01.T01 AT3G06130 48.485 66 34 0 29 94 9 74 1.91e-13 67.0
MsG0380016326.01.T01 AT3G56240 50.000 66 33 0 30 95 3 68 3.02e-13 62.8
MsG0380016326.01.T01 AT3G56240 50.000 66 33 0 30 95 3 68 3.02e-13 62.8
MsG0380016326.01.T01 AT3G06130 47.761 67 35 0 28 94 8 74 3.56e-13 65.9
MsG0380016326.01.T01 AT5G19090 46.269 67 36 0 28 94 8 74 3.42e-12 63.5
MsG0380016326.01.T01 AT5G19090 46.269 67 36 0 28 94 8 74 3.42e-12 63.5
MsG0380016326.01.T01 AT5G19090 46.269 67 36 0 28 94 8 74 3.42e-12 63.5
MsG0380016326.01.T01 AT5G19090 46.269 67 36 0 28 94 8 74 4.22e-12 63.2
MsG0380016326.01.T01 AT5G19090 46.269 67 36 0 28 94 8 74 4.22e-12 63.2
MsG0380016326.01.T01 AT1G56210 49.231 65 33 0 34 98 44 108 4.91e-12 62.8
MsG0380016326.01.T01 AT1G23000 42.857 70 40 0 29 98 12 81 1.48e-11 61.2
MsG0380016326.01.T01 AT1G23000 42.857 70 40 0 29 98 72 141 1.50e-11 61.6
MsG0380016326.01.T01 AT5G37860 40.845 71 42 0 29 99 9 79 3.78e-11 59.7

Find 41 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTATCATCATCTACATAA+TGG 0.292404 3:-82035990 None:intergenic
TACATAATGGAGCAGGCATT+TGG 0.304332 3:-82035977 None:intergenic
AGAGTACTTATCTGATCTAA+TGG 0.306737 3:+82035281 MsG0380016326.01.T01:CDS
TAAAATCAGTGGAGATAAAC+AGG 0.372946 3:+82035711 MsG0380016326.01.T01:CDS
TTGCGATGGTTGCGAACTTA+AGG 0.383976 3:+82035377 MsG0380016326.01.T01:CDS
ACCATGTTGTATGGCACATA+AGG 0.433890 3:-82035824 None:intergenic
AGCAAAGTCAACAGGAAAGA+AGG 0.439458 3:+82035790 MsG0380016326.01.T01:CDS
CAGGAAAGAAGGCAGAGATT+TGG 0.440709 3:+82035801 MsG0380016326.01.T01:CDS
ACATAATGGAGCAGGCATTT+GGG 0.448683 3:-82035976 None:intergenic
GGGGTATGTAGAGGCAAATA+AGG 0.452605 3:+82035757 MsG0380016326.01.T01:CDS
TCCTGGATATGTAAGGAGAT+TGG 0.456785 3:+82035889 MsG0380016326.01.T01:CDS
TCCAATCTCCTTACATATCC+AGG 0.463407 3:-82035890 None:intergenic
CTAAAGAAAGCAAAGTCAAC+AGG 0.471841 3:+82035782 MsG0380016326.01.T01:CDS
GAGTACTTATCTGATCTAAT+GGG 0.482086 3:+82035282 MsG0380016326.01.T01:CDS
AAAGCCCTCTCTTCAATGAA+TGG 0.498175 3:+82035405 MsG0380016326.01.T01:CDS
ATTACAGACCGTAGAGTTGA+AGG 0.499504 3:+82035344 MsG0380016326.01.T01:CDS
TATGACAAGAAGGCTCCTCC+TGG 0.508900 3:+82035872 MsG0380016326.01.T01:CDS
CGTAGAGTTGAAGGTGAGGA+TGG 0.509056 3:+82035353 MsG0380016326.01.T01:CDS
CTTACCATTCATTGAAGAGA+GGG 0.529075 3:-82035409 None:intergenic
ATATACACAGAAATGGGAGT+TGG 0.529253 3:+82035249 None:intergenic
AGGCTCCTCCTGGATATGTA+AGG 0.531905 3:+82035882 MsG0380016326.01.T01:CDS
GGAGCCTTCTTGTCATAAGA+AGG 0.533318 3:-82035866 None:intergenic
GGATGAGCCACCATGTTGTA+TGG 0.533759 3:-82035833 None:intergenic
TCATAAGAAGGAGCAGCGTA+TGG 0.549598 3:-82035854 None:intergenic
TACACAGAAATGGGAGTTGG+AGG 0.552311 3:+82035252 None:intergenic
CATCATCTACATAATGGAGC+AGG 0.555456 3:-82035984 None:intergenic
TGCTCCTTCTTATGACAAGA+AGG 0.562724 3:+82035862 MsG0380016326.01.T01:CDS
GTAAGGAGATTGGAGACCAC+TGG 0.572647 3:+82035899 MsG0380016326.01.T01:CDS
TTATCTGATCTAATGGGCAG+TGG 0.573748 3:+82035288 MsG0380016326.01.T01:CDS
CATCCTCACCTTCAACTCTA+CGG 0.585179 3:-82035352 None:intergenic
GACAGTAACGGGGTATGTAG+AGG 0.602619 3:+82035748 MsG0380016326.01.T01:CDS
ACTTACCATTCATTGAAGAG+AGG 0.616934 3:-82035410 None:intergenic
CATCACTAAACATGGTGGTG+AGG 0.624282 3:-82035949 None:intergenic
GCCTTATGTGCCATACAACA+TGG 0.647674 3:+82035823 MsG0380016326.01.T01:CDS
ATATGCTCTAACAGTTCCAG+TGG 0.649114 3:-82035915 None:intergenic
AAGGTGAGGATGGATTGCGA+TGG 0.658836 3:+82035363 MsG0380016326.01.T01:CDS
CTAAACATGGTGGTGAGGTG+TGG 0.665051 3:-82035944 None:intergenic
AGACCGTAGAGTTGAAGGTG+AGG 0.688480 3:+82035349 MsG0380016326.01.T01:CDS
TGATGCAGGAGTAAAATCAG+TGG 0.694945 3:+82035700 MsG0380016326.01.T01:intron
TTATGTGCCATACAACATGG+TGG 0.751150 3:+82035826 MsG0380016326.01.T01:CDS
AATCTCCTTACATATCCAGG+AGG 0.766746 3:-82035887 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAGAAAGATAAATTAATAG+AGG - Chr3:82035445-82035464 None:intergenic 20.0%
!! GAGAAAGATAAATTAATAGA+GGG - Chr3:82035444-82035463 None:intergenic 20.0%
! TCAAATTATAATTAGTGCGT+CGG + Chr3:82035555-82035574 MsG0380016326.01.T01:intron 25.0%
ACAACAAAAAGTGACAGTAA+CGG + Chr3:82035736-82035755 MsG0380016326.01.T01:CDS 30.0%
GAGTACTTATCTGATCTAAT+GGG + Chr3:82035282-82035301 MsG0380016326.01.T01:CDS 30.0%
TAAAATCAGTGGAGATAAAC+AGG + Chr3:82035711-82035730 MsG0380016326.01.T01:CDS 30.0%
TATCTTTCTCTATATAGCAC+CGG + Chr3:82035454-82035473 MsG0380016326.01.T01:intron 30.0%
! AGAGTACTTATCTGATCTAA+TGG + Chr3:82035281-82035300 MsG0380016326.01.T01:CDS 30.0%
! TCTTCTTCTTGATTTTCTGA+TGG - Chr3:82035320-82035339 None:intergenic 30.0%
!!! TTTTTTTTTTGTGGTGATGC+AGG + Chr3:82035686-82035705 MsG0380016326.01.T01:intron 30.0%
AACAAAAAGTGACAGTAACG+GGG + Chr3:82035738-82035757 MsG0380016326.01.T01:CDS 35.0%
ACTTACCATTCATTGAAGAG+AGG - Chr3:82035413-82035432 None:intergenic 35.0%
CAACAAAAAGTGACAGTAAC+GGG + Chr3:82035737-82035756 MsG0380016326.01.T01:CDS 35.0%
CTAAAGAAAGCAAAGTCAAC+AGG + Chr3:82035782-82035801 MsG0380016326.01.T01:CDS 35.0%
CTTACCATTCATTGAAGAGA+GGG - Chr3:82035412-82035431 None:intergenic 35.0%
GCTTGCTTGCATTATATGTA+TGG + Chr3:82035611-82035630 MsG0380016326.01.T01:intron 35.0%
! GTTTTCATCACTAAACATGG+TGG - Chr3:82035957-82035976 None:intergenic 35.0%
! TCTTCTTGATTTTCTGATGG+TGG - Chr3:82035317-82035336 None:intergenic 35.0%
! TGGGTTTTCATCACTAAACA+TGG - Chr3:82035960-82035979 None:intergenic 35.0%
AAAGCCCTCTCTTCAATGAA+TGG + Chr3:82035405-82035424 MsG0380016326.01.T01:CDS 40.0%
AATCTCCTTACATATCCAGG+AGG - Chr3:82035890-82035909 None:intergenic 40.0%
ACCATGTTGTATGGCACATA+AGG - Chr3:82035827-82035846 None:intergenic 40.0%
ACGTCATCAATATGAAGTGC+CGG - Chr3:82035476-82035495 None:intergenic 40.0%
AGCAAAGTCAACAGGAAAGA+AGG + Chr3:82035790-82035809 MsG0380016326.01.T01:CDS 40.0%
ATATGCTCTAACAGTTCCAG+TGG - Chr3:82035918-82035937 None:intergenic 40.0%
ATTACAGACCGTAGAGTTGA+AGG + Chr3:82035344-82035363 MsG0380016326.01.T01:CDS 40.0%
TCCAATCTCCTTACATATCC+AGG - Chr3:82035893-82035912 None:intergenic 40.0%
TCCTGGATATGTAAGGAGAT+TGG + Chr3:82035889-82035908 MsG0380016326.01.T01:CDS 40.0%
TGATGCAGGAGTAAAATCAG+TGG + Chr3:82035700-82035719 MsG0380016326.01.T01:intron 40.0%
TGCTCCTTCTTATGACAAGA+AGG + Chr3:82035862-82035881 MsG0380016326.01.T01:CDS 40.0%
TTATGTGCCATACAACATGG+TGG + Chr3:82035826-82035845 MsG0380016326.01.T01:CDS 40.0%
! ACATAATGGAGCAGGCATTT+GGG - Chr3:82035979-82035998 None:intergenic 40.0%
! TACATAATGGAGCAGGCATT+TGG - Chr3:82035980-82035999 None:intergenic 40.0%
! TTATCTGATCTAATGGGCAG+TGG + Chr3:82035288-82035307 MsG0380016326.01.T01:CDS 40.0%
CAGGAAAGAAGGCAGAGATT+TGG + Chr3:82035801-82035820 MsG0380016326.01.T01:CDS 45.0%
CATCCTCACCTTCAACTCTA+CGG - Chr3:82035355-82035374 None:intergenic 45.0%
GCCTTATGTGCCATACAACA+TGG + Chr3:82035823-82035842 MsG0380016326.01.T01:CDS 45.0%
GGAGCCTTCTTGTCATAAGA+AGG - Chr3:82035869-82035888 None:intergenic 45.0%
GGGGTATGTAGAGGCAAATA+AGG + Chr3:82035757-82035776 MsG0380016326.01.T01:CDS 45.0%
TCATAAGAAGGAGCAGCGTA+TGG - Chr3:82035857-82035876 None:intergenic 45.0%
TTGCGATGGTTGCGAACTTA+AGG + Chr3:82035377-82035396 MsG0380016326.01.T01:CDS 45.0%
! CATCACTAAACATGGTGGTG+AGG - Chr3:82035952-82035971 None:intergenic 45.0%
!!! TTTTTTTTTTTTTTTTTTTG+TGG + Chr3:82035677-82035696 MsG0380016326.01.T01:intron 5.0%
AGACCGTAGAGTTGAAGGTG+AGG + Chr3:82035349-82035368 MsG0380016326.01.T01:CDS 50.0%
AGGCTCCTCCTGGATATGTA+AGG + Chr3:82035882-82035901 MsG0380016326.01.T01:CDS 50.0%
CGTAGAGTTGAAGGTGAGGA+TGG + Chr3:82035353-82035372 MsG0380016326.01.T01:CDS 50.0%
CGTCTGACATCACTGACACA+TGG + Chr3:82035502-82035521 MsG0380016326.01.T01:intron 50.0%
GGATGAGCCACCATGTTGTA+TGG - Chr3:82035836-82035855 None:intergenic 50.0%
GTAAGGAGATTGGAGACCAC+TGG + Chr3:82035899-82035918 MsG0380016326.01.T01:CDS 50.0%
GTGTCAGTGATGTCAGACGT+TGG - Chr3:82035501-82035520 None:intergenic 50.0%
TATGACAAGAAGGCTCCTCC+TGG + Chr3:82035872-82035891 MsG0380016326.01.T01:CDS 50.0%
! CTAAACATGGTGGTGAGGTG+TGG - Chr3:82035947-82035966 None:intergenic 50.0%
! GACAGTAACGGGGTATGTAG+AGG + Chr3:82035748-82035767 MsG0380016326.01.T01:CDS 50.0%
!! AAGGTGAGGATGGATTGCGA+TGG + Chr3:82035363-82035382 MsG0380016326.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 82035261 82036000 82035261 ID=MsG0380016326.01;Name=MsG0380016326.01
Chr3 mRNA 82035261 82036000 82035261 ID=MsG0380016326.01.T01;Parent=MsG0380016326.01;Name=MsG0380016326.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|0.5|2|0|152
Chr3 exon 82035261 82035426 82035261 ID=MsG0380016326.01.T01:exon:11595;Parent=MsG0380016326.01.T01
Chr3 exon 82035708 82036000 82035708 ID=MsG0380016326.01.T01:exon:11596;Parent=MsG0380016326.01.T01
Chr3 CDS 82035261 82035426 82035261 ID=MsG0380016326.01.T01:cds;Parent=MsG0380016326.01.T01
Chr3 CDS 82035708 82036000 82035708 ID=MsG0380016326.01.T01:cds;Parent=MsG0380016326.01.T01
Gene Sequence

>MsG0380016326.01.T01

ATGGGAGTTGGAGGCACATTAGAGTACTTATCTGATCTAATGGGCAGTGGTCACCACCATCAGAAAATCAAGAAGAAGAAGCAATTACAGACCGTAGAGTTGAAGGTGAGGATGGATTGCGATGGTTGCGAACTTAAGGTCAAGAAAGCCCTCTCTTCAATGAATGGAGTAAAATCAGTGGAGATAAACAGGAAACAACAAAAAGTGACAGTAACGGGGTATGTAGAGGCAAATAAGGTGCTAAAGAAAGCAAAGTCAACAGGAAAGAAGGCAGAGATTTGGCCTTATGTGCCATACAACATGGTGGCTCATCCATACGCTGCTCCTTCTTATGACAAGAAGGCTCCTCCTGGATATGTAAGGAGATTGGAGACCACTGGAACTGTTAGAGCATATGAAGAGCCACACCTCACCACCATGTTTAGTGATGAAAACCCAAATGCCTGCTCCATTATGTAG

Protein sequence

>MsG0380016326.01.T01

MGVGGTLEYLSDLMGSGHHHQKIKKKKQLQTVELKVRMDCDGCELKVKKALSSMNGVKSVEINRKQQKVTVTGYVEANKVLKKAKSTGKKAEIWPYVPYNMVAHPYAAPSYDKKAPPGYVRRLETTGTVRAYEEPHLTTMFSDENPNACSIM*