AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016710.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016710.01.T01 MTR_3g093860 92.357 157 9 2 1 155 1 156 2.85e-103 292
MsG0380016710.01.T01 MTR_1g049100 60.448 134 52 1 14 146 4 137 6.25e-55 171
MsG0380016710.01.T01 MTR_3g078613 60.769 130 50 1 19 148 24 152 1.02e-54 171
MsG0380016710.01.T01 MTR_3g078613 61.069 131 49 2 19 148 24 153 1.86e-53 168
MsG0380016710.01.T01 MTR_5g036480 60.606 132 47 2 19 146 17 147 5.17e-52 165
MsG0380016710.01.T01 MTR_8g038620 57.353 136 51 1 19 147 10 145 1.15e-51 162
MsG0380016710.01.T01 MTR_4g106590 60.305 131 50 2 19 148 27 156 1.71e-51 163
MsG0380016710.01.T01 MTR_7g490310 59.091 132 46 2 19 142 10 141 1.57e-50 162
MsG0380016710.01.T01 MTR_3g088630 53.191 141 48 2 19 142 23 162 7.22e-46 150
MsG0380016710.01.T01 MTR_3g015490 52.143 140 50 3 19 147 5 138 6.15e-43 140
MsG0380016710.01.T01 MTR_6g007460 40.659 182 54 3 19 147 12 192 6.86e-37 126
MsG0380016710.01.T01 MTR_2g050020 91.379 58 4 1 1 58 35 91 1.00e-30 107
MsG0380016710.01.T01 MTR_4g051330 56.863 51 22 0 97 147 77 127 3.81e-16 71.2
MsG0380016710.01.T01 MTR_4g061360 34.959 123 70 1 20 142 91 203 8.83e-16 73.9
MsG0380016710.01.T01 MTR_1g067110 34.959 123 70 1 20 142 87 199 6.61e-15 71.2
MsG0380016710.01.T01 MTR_1g067110 34.959 123 70 1 20 142 87 199 8.04e-15 70.9
MsG0380016710.01.T01 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.48e-14 68.2
MsG0380016710.01.T01 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.48e-14 68.2
MsG0380016710.01.T01 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.63e-14 68.2
MsG0380016710.01.T01 MTR_3g092780 30.645 124 74 2 20 142 58 170 7.93e-14 68.2
MsG0380016710.01.T01 MTR_8g024260 30.894 123 75 1 20 142 38 150 3.23e-13 66.2
MsG0380016710.01.T01 MTR_7g118260 30.894 123 75 1 20 142 16 128 4.41e-13 65.9
MsG0380016710.01.T01 MTR_7g118260 30.894 123 75 1 20 142 16 128 5.16e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016710.01.T01 AT3G56380 72.308 130 36 0 19 148 21 150 2.43e-64 194
MsG0380016710.01.T01 AT3G56380 72.519 131 35 1 19 148 21 151 4.88e-63 191
MsG0380016710.01.T01 AT2G40670 70.896 134 33 2 19 148 30 161 7.35e-63 191
MsG0380016710.01.T01 AT2G40670 71.111 135 32 3 19 148 30 162 1.50e-61 187
MsG0380016710.01.T01 AT1G10470 61.719 128 48 1 19 146 21 147 3.43e-53 169
MsG0380016710.01.T01 AT1G59940 58.647 133 54 1 14 146 29 160 4.99e-53 168
MsG0380016710.01.T01 AT1G10470 61.719 128 48 1 19 146 35 161 6.36e-53 168
MsG0380016710.01.T01 AT1G59940 58.647 133 54 1 14 146 121 252 7.45e-52 167
MsG0380016710.01.T01 AT3G48100 57.364 129 55 0 19 147 26 154 1.34e-51 162
MsG0380016710.01.T01 AT5G62920 58.955 134 54 1 19 152 26 158 3.22e-51 162
MsG0380016710.01.T01 AT3G57040 53.623 138 55 1 19 147 10 147 6.46e-51 162
MsG0380016710.01.T01 AT3G57040 53.623 138 55 1 19 147 10 147 6.46e-51 162
MsG0380016710.01.T01 AT2G41310 51.724 145 63 2 10 147 1 145 2.33e-50 161
MsG0380016710.01.T01 AT1G74890 59.091 132 53 1 19 150 19 149 5.99e-50 159
MsG0380016710.01.T01 AT1G19050 58.015 131 52 2 19 148 25 153 3.46e-49 157
MsG0380016710.01.T01 AT1G10470 50.000 74 33 3 75 146 14 85 3.19e-15 69.7
MsG0380016710.01.T01 AT5G24470 31.452 124 73 2 20 142 52 164 7.07e-14 68.2
MsG0380016710.01.T01 AT5G02810 31.707 123 74 1 20 142 80 192 2.13e-12 64.3
MsG0380016710.01.T01 AT5G60100 33.333 123 72 1 20 142 66 178 2.30e-12 63.9
MsG0380016710.01.T01 AT5G60100 33.333 123 72 1 20 142 66 178 2.30e-12 63.9
MsG0380016710.01.T01 AT5G60100 33.333 123 72 1 20 142 66 178 2.30e-12 63.9
MsG0380016710.01.T01 AT5G60100 33.333 123 72 1 20 142 66 178 2.32e-12 63.9
MsG0380016710.01.T01 AT5G60100 33.333 123 72 1 20 142 66 178 5.29e-12 62.8
MsG0380016710.01.T01 AT5G60100 33.333 123 72 1 20 142 66 178 5.29e-12 62.8
MsG0380016710.01.T01 AT5G60100 33.333 123 72 1 20 142 66 178 5.29e-12 62.8
MsG0380016710.01.T01 AT2G46790 28.226 124 79 1 19 142 38 151 1.90e-11 61.2
MsG0380016710.01.T01 AT2G46790 28.226 124 79 1 19 142 38 151 2.29e-11 61.2
MsG0380016710.01.T01 AT5G07210 30.081 123 72 3 20 142 18 126 3.30e-11 60.8

Find 39 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGAAGGCATTGGAGTATT+TGG 0.158019 3:-86903553 MsG0380016710.01.T01:CDS
GACAGGCCAGCTGATGAATT+TGG 0.201243 3:-86902932 MsG0380016710.01.T01:CDS
ACAGTGACAACTGCTGAAAA+TGG 0.248549 3:-86903576 MsG0380016710.01.T01:intron
CTGAAGGCATTGGAGTATTT+GGG 0.249714 3:-86903552 MsG0380016710.01.T01:CDS
GTAGGGACCCAAAGTGAATT+TGG 0.331645 3:-86903356 MsG0380016710.01.T01:intron
AATTACTTGTTGATCCTTGT+TGG 0.359191 3:+86903108 None:intergenic
TCAAAGTGGGTGATGGAAAA+TGG 0.361841 3:-86903875 MsG0380016710.01.T01:CDS
TGAGTTGCTTAAGAAAATTA+AGG 0.374516 3:-86903296 MsG0380016710.01.T01:intron
ACAGGCCAGCTGATGAATTT+GGG 0.393986 3:-86902931 MsG0380016710.01.T01:CDS
TACTGCATGCCAGGGATGAC+TGG 0.411671 3:-86903322 MsG0380016710.01.T01:CDS
GTGATGACCAAATTCACTTT+GGG 0.424646 3:+86903349 None:intergenic
ATCACAGACTACTGCATGCC+AGG 0.428078 3:-86903331 MsG0380016710.01.T01:CDS
TTCTTCTTCAAAGTGGGTGA+TGG 0.456607 3:-86903882 MsG0380016710.01.T01:CDS
CTGATTCTTCTTCTTCAAAG+TGG 0.467692 3:-86903889 MsG0380016710.01.T01:CDS
TGATTCTTCTTCTTCAAAGT+GGG 0.471982 3:-86903888 MsG0380016710.01.T01:CDS
ACCTTCCCAAATTCATCAGC+TGG 0.476038 3:+86902926 None:intergenic
TCAGATGACATGATCACAAC+AGG 0.480037 3:+86903138 None:intergenic
TCAGCAATCATCTGTGATGA+AGG 0.481432 3:-86903166 MsG0380016710.01.T01:intron
TGTGATGACCAAATTCACTT+TGG 0.486577 3:+86903348 None:intergenic
GAACAGAACACTTTGGAGAA+TGG 0.490659 3:-86903516 MsG0380016710.01.T01:CDS
CAGTGACAACTGCTGAAAAT+GGG 0.502968 3:-86903575 MsG0380016710.01.T01:CDS
TTTACATCAGATTGTTTGAG+AGG 0.513388 3:+86902961 None:intergenic
TGAAAATGGGCTGAAGGCAT+TGG 0.515465 3:-86903562 MsG0380016710.01.T01:CDS
AACTCATAGCCAGTCATCCC+TGG 0.516855 3:+86903313 None:intergenic
AAAAGCAGGTGTATGGAAGA+AGG 0.535716 3:-86903006 MsG0380016710.01.T01:intron
AAAGCAGGTGTATGGAAGAA+GGG 0.542181 3:-86903005 MsG0380016710.01.T01:intron
AACTGCTGAAAATGGGCTGA+AGG 0.568007 3:-86903568 MsG0380016710.01.T01:CDS
CTCAAGAATTCCTCTTGCAA+AGG 0.589496 3:-86903788 MsG0380016710.01.T01:intron
GCCAGCTGATGAATTTGGGA+AGG 0.598518 3:-86902927 MsG0380016710.01.T01:CDS
AAGCAGGTGTATGGAAGAAG+GGG 0.612241 3:-86903004 MsG0380016710.01.T01:intron
TCACAGACTACTGCATGCCA+GGG 0.612620 3:-86903330 MsG0380016710.01.T01:CDS
CTTTGCAAGAGGAATTCTTG+AGG 0.621330 3:+86903789 None:intergenic
TCTGATGTAAAACAACTGAC+AGG 0.621647 3:-86902949 MsG0380016710.01.T01:CDS
TCATCAACAGCAAGAACATG+AGG 0.625979 3:+86903845 None:intergenic
AAGTGGTGAACAGAACACTT+TGG 0.626039 3:-86903523 MsG0380016710.01.T01:CDS
GAGTATTTGGGCTTGTCAAG+TGG 0.627865 3:-86903540 MsG0380016710.01.T01:CDS
GGTGTACGTACCTTTGCAAG+AGG 0.636893 3:+86903778 None:intergenic
GCAATCATCTGTGATGAAGG+AGG 0.667691 3:-86903163 MsG0380016710.01.T01:CDS
CATCTGAGAATATACCAACA+AGG 0.683899 3:-86903122 MsG0380016710.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGAATATAACAGAAAA+TGG + Chr3:86903070-86903089 None:intergenic 15.0%
!! AGAAAAAGATACAAAAAAAA+TGG + Chr3:86903749-86903768 None:intergenic 15.0%
!! CTGAAAATAATATCCAATTT+GGG + Chr3:86903626-86903645 None:intergenic 20.0%
!! TGAAAATAATATCCAATTTG+GGG + Chr3:86903625-86903644 None:intergenic 20.0%
!!! TGATGTTTGATGTTTTTTTT+TGG - Chr3:86903157-86903176 MsG0380016710.01.T01:CDS 20.0%
!!! TTTTTTTTTGGTCCATATTT+TGG - Chr3:86903169-86903188 MsG0380016710.01.T01:intron 20.0%
! GCTGAAAATAATATCCAATT+TGG + Chr3:86903627-86903646 None:intergenic 25.0%
! TGAGTTGCTTAAGAAAATTA+AGG - Chr3:86903512-86903531 MsG0380016710.01.T01:intron 25.0%
!!! GTGTTTTGTTAGTAACATTT+TGG + Chr3:86903350-86903369 None:intergenic 25.0%
AAATAATATCCAATTTGGGG+TGG + Chr3:86903622-86903641 None:intergenic 30.0%
CAAACAGAATCACCAAAATA+TGG + Chr3:86903184-86903203 None:intergenic 30.0%
GTGATTCTGTTTGATTGAAT+GGG - Chr3:86903191-86903210 MsG0380016710.01.T01:intron 30.0%
TGATTCTTCTTCTTCAAAGT+GGG - Chr3:86902920-86902939 MsG0380016710.01.T01:CDS 30.0%
TTTACATCAGATTGTTTGAG+AGG + Chr3:86903850-86903869 None:intergenic 30.0%
! AATTACTTGTTGATCCTTGT+TGG + Chr3:86903703-86903722 None:intergenic 30.0%
!!! GACTATTTCTGGTTTTTGTA+GGG - Chr3:86903435-86903454 MsG0380016710.01.T01:intron 30.0%
!!! TGACTATTTCTGGTTTTTGT+AGG - Chr3:86903434-86903453 MsG0380016710.01.T01:intron 30.0%
AAAAATGGCATTGTTAGTGC+TGG + Chr3:86903734-86903753 None:intergenic 35.0%
AAAATGGTTGAGTGAGAGAT+AGG + Chr3:86903054-86903073 None:intergenic 35.0%
CATCTGAGAATATACCAACA+AGG - Chr3:86903686-86903705 MsG0380016710.01.T01:intron 35.0%
CTGATTCTTCTTCTTCAAAG+TGG - Chr3:86902919-86902938 MsG0380016710.01.T01:CDS 35.0%
GGTGATTCTGTTTGATTGAA+TGG - Chr3:86903190-86903209 MsG0380016710.01.T01:intron 35.0%
GTGATGACCAAATTCACTTT+GGG + Chr3:86903462-86903481 None:intergenic 35.0%
TCTGATGTAAAACAACTGAC+AGG - Chr3:86903859-86903878 MsG0380016710.01.T01:CDS 35.0%
TGCTAACAAGTGACTATTTC+TGG - Chr3:86903424-86903443 MsG0380016710.01.T01:intron 35.0%
TGTGATGACCAAATTCACTT+TGG + Chr3:86903463-86903482 None:intergenic 35.0%
!! TGATTGAATGGGCTTTTTGT+TGG - Chr3:86903202-86903221 MsG0380016710.01.T01:intron 35.0%
AAAAGCAGGTGTATGGAAGA+AGG - Chr3:86903802-86903821 MsG0380016710.01.T01:CDS 40.0%
AAAGCAGGTGTATGGAAGAA+GGG - Chr3:86903803-86903822 MsG0380016710.01.T01:CDS 40.0%
AAGTGGTGAACAGAACACTT+TGG - Chr3:86903285-86903304 MsG0380016710.01.T01:intron 40.0%
ACAGTGACAACTGCTGAAAA+TGG - Chr3:86903232-86903251 MsG0380016710.01.T01:intron 40.0%
CAGCACAACAATGATTGAGT+AGG + Chr3:86903781-86903800 None:intergenic 40.0%
CAGTGACAACTGCTGAAAAT+GGG - Chr3:86903233-86903252 MsG0380016710.01.T01:intron 40.0%
CATTGTTGTGCTGAAAAAGC+AGG - Chr3:86903788-86903807 MsG0380016710.01.T01:intron 40.0%
CTCAAGAATTCCTCTTGCAA+AGG - Chr3:86903020-86903039 MsG0380016710.01.T01:intron 40.0%
CTTTGCAAGAGGAATTCTTG+AGG + Chr3:86903022-86903041 None:intergenic 40.0%
TAACACTAACCACCCCAAAT+TGG - Chr3:86903610-86903629 MsG0380016710.01.T01:intron 40.0%
TCAGATGACATGATCACAAC+AGG + Chr3:86903673-86903692 None:intergenic 40.0%
TCAGCAATCATCTGTGATGA+AGG - Chr3:86903642-86903661 MsG0380016710.01.T01:intron 40.0%
TCATCAACAGCAAGAACATG+AGG + Chr3:86902966-86902985 None:intergenic 40.0%
TTCTTCTTCAAAGTGGGTGA+TGG - Chr3:86902926-86902945 MsG0380016710.01.T01:CDS 40.0%
! GAACAGAACACTTTGGAGAA+TGG - Chr3:86903292-86903311 MsG0380016710.01.T01:intron 40.0%
!! CTGAAGGCATTGGAGTATTT+GGG - Chr3:86903256-86903275 MsG0380016710.01.T01:intron 40.0%
!! TCAAAGTGGGTGATGGAAAA+TGG - Chr3:86902933-86902952 MsG0380016710.01.T01:CDS 40.0%
AAGCAGGTGTATGGAAGAAG+GGG - Chr3:86903804-86903823 MsG0380016710.01.T01:CDS 45.0%
ACAGGCCAGCTGATGAATTT+GGG - Chr3:86903877-86903896 MsG0380016710.01.T01:CDS 45.0%
ACCTTCCCAAATTCATCAGC+TGG + Chr3:86903885-86903904 None:intergenic 45.0%
GAGTATTTGGGCTTGTCAAG+TGG - Chr3:86903268-86903287 MsG0380016710.01.T01:intron 45.0%
GCAATCATCTGTGATGAAGG+AGG - Chr3:86903645-86903664 MsG0380016710.01.T01:intron 45.0%
GTAGGGACCCAAAGTGAATT+TGG - Chr3:86903452-86903471 MsG0380016710.01.T01:intron 45.0%
GTGCTGAAAAAGCAGGTGTA+TGG - Chr3:86903795-86903814 MsG0380016710.01.T01:CDS 45.0%
!! AACTGCTGAAAATGGGCTGA+AGG - Chr3:86903240-86903259 MsG0380016710.01.T01:intron 45.0%
!! GCTGAAGGCATTGGAGTATT+TGG - Chr3:86903255-86903274 MsG0380016710.01.T01:intron 45.0%
!! TGAAAATGGGCTGAAGGCAT+TGG - Chr3:86903246-86903265 MsG0380016710.01.T01:intron 45.0%
AACTCATAGCCAGTCATCCC+TGG + Chr3:86903498-86903517 None:intergenic 50.0%
ATCACAGACTACTGCATGCC+AGG - Chr3:86903477-86903496 MsG0380016710.01.T01:intron 50.0%
GACAGGCCAGCTGATGAATT+TGG - Chr3:86903876-86903895 MsG0380016710.01.T01:CDS 50.0%
GCCAGCTGATGAATTTGGGA+AGG - Chr3:86903881-86903900 MsG0380016710.01.T01:CDS 50.0%
GGTGTACGTACCTTTGCAAG+AGG + Chr3:86903033-86903052 None:intergenic 50.0%
TCACAGACTACTGCATGCCA+GGG - Chr3:86903478-86903497 MsG0380016710.01.T01:intron 50.0%
TACTGCATGCCAGGGATGAC+TGG - Chr3:86903486-86903505 MsG0380016710.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 86902912 86903918 86902912 ID=MsG0380016710.01;Name=MsG0380016710.01
Chr3 mRNA 86902912 86903918 86902912 ID=MsG0380016710.01.T01;Parent=MsG0380016710.01;Name=MsG0380016710.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|5|0|155
Chr3 exon 86903789 86903918 86903789 ID=MsG0380016710.01.T01:exon:22402;Parent=MsG0380016710.01.T01
Chr3 exon 86903515 86903594 86903515 ID=MsG0380016710.01.T01:exon:22401;Parent=MsG0380016710.01.T01
Chr3 exon 86903297 86903374 86903297 ID=MsG0380016710.01.T01:exon:22400;Parent=MsG0380016710.01.T01
Chr3 exon 86903114 86903184 86903114 ID=MsG0380016710.01.T01:exon:22399;Parent=MsG0380016710.01.T01
Chr3 exon 86902912 86903020 86902912 ID=MsG0380016710.01.T01:exon:22398;Parent=MsG0380016710.01.T01
Chr3 CDS 86903789 86903918 86903789 ID=MsG0380016710.01.T01:cds;Parent=MsG0380016710.01.T01
Chr3 CDS 86903515 86903594 86903515 ID=MsG0380016710.01.T01:cds;Parent=MsG0380016710.01.T01
Chr3 CDS 86903297 86903374 86903297 ID=MsG0380016710.01.T01:cds;Parent=MsG0380016710.01.T01
Chr3 CDS 86903114 86903184 86903114 ID=MsG0380016710.01.T01:cds;Parent=MsG0380016710.01.T01
Chr3 CDS 86902912 86903020 86902912 ID=MsG0380016710.01.T01:cds;Parent=MsG0380016710.01.T01
Gene Sequence

>MsG0380016710.01.T01

ATGGCTTCTGATTCTTCTTCTTCAAAGTGGGTGATGGAAAATGGTGACATTCCTCATGTTCTTGCTGTTGATGACAATATCATTGATCGCACACTTGTTGAGAAACTCCTCAAGAATTCCTCTTGCAAAGTGACAACTGCTGAAAATGGGCTGAAGGCATTGGAGTATTTGGGCTTGTCAAGTGGTGAACAGAACACTTTGGAGAATGGAGGACCCAAAGTGAATTTGGTCATCACAGACTACTGCATGCCAGGGATGACTGGCTATGAGTTGCTTAAGAAAATTAAGCAATCATCTGTGATGAAGGAGGTTCCTGTTGTGATCATGTCATCTGAGAATATACCAACAAGGATCAACAAGTGTATGGAAGAAGGGGCTCAGATGTTCATACAAAAGCCTCTCAAACAATCTGATGTAAAACAACTGACAGGCCAGCTGATGAATTTGGGAAGGTTGCAAAAGAATTGA

Protein sequence

>MsG0380016710.01.T01

MASDSSSSKWVMENGDIPHVLAVDDNIIDRTLVEKLLKNSSCKVTTAENGLKALEYLGLSSGEQNTLENGGPKVNLVITDYCMPGMTGYELLKKIKQSSVMKEVPVVIMSSENIPTRINKCMEEGAQMFIQKPLKQSDVKQLTGQLMNLGRLQKN*