AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017402.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017402.01.T01 MTR_3g106750 95.420 131 3 2 1 130 1 129 2.43e-63 189
MsG0380017402.01.T01 MTR_3g106740 74.615 130 31 2 1 129 1 129 2.14e-56 172
MsG0380017402.01.T01 MTR_1g012690 57.325 157 37 4 1 130 1 154 2.02e-50 157
MsG0380017402.01.T01 MTR_1g012700 56.627 166 34 4 1 130 1 164 2.24e-49 155
MsG0380017402.01.T01 MTR_1g012710 60.784 153 35 3 1 130 1 151 1.04e-44 143
MsG0380017402.01.T01 MTR_1g012630 79.762 84 16 1 47 130 128 210 1.80e-41 137
MsG0380017402.01.T01 MTR_4g108150 50.820 122 56 2 9 130 6 123 6.92e-33 112
MsG0380017402.01.T01 MTR_4g101260 63.095 84 30 1 47 130 50 132 5.06e-31 107
MsG0380017402.01.T01 MTR_4g101330 40.588 170 58 6 1 129 1 168 2.69e-29 104
MsG0380017402.01.T01 MTR_4g101280 56.818 88 36 2 44 130 57 143 1.01e-28 102
MsG0380017402.01.T01 MTR_2g026760 47.619 126 61 3 4 129 6 126 1.43e-28 101
MsG0380017402.01.T01 MTR_4g101310 57.647 85 34 2 47 130 51 134 9.25e-28 99.8
MsG0380017402.01.T01 MTR_4g101270 57.143 84 33 3 47 129 105 186 1.63e-22 87.8
MsG0380017402.01.T01 MTR_5g012340 47.619 84 41 2 47 129 95 176 1.76e-22 87.4
MsG0380017402.01.T01 MTR_5g012380 46.429 84 42 2 47 129 88 169 8.17e-22 85.5
MsG0380017402.01.T01 MTR_2g026930 48.750 80 38 2 50 129 122 198 1.62e-16 72.4
MsG0380017402.01.T01 MTR_2g026920 48.750 80 38 2 50 129 118 194 1.75e-16 72.4
MsG0380017402.01.T01 MTR_3g082100 46.512 86 45 1 44 129 251 335 1.30e-15 72.0
MsG0380017402.01.T01 MTR_2g026775 41.772 79 40 3 54 130 686 760 7.00e-13 64.7
MsG0380017402.01.T01 MTR_4g115360 48.235 85 41 3 47 130 151 233 2.70e-11 58.9
MsG0380017402.01.T01 MTR_2g026820 52.778 72 27 4 52 121 542 608 3.79e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017402.01.T01 AT2G45180 69.048 84 25 1 47 130 52 134 4.36e-34 115
MsG0380017402.01.T01 AT4G12490 60.000 95 36 2 37 130 89 182 5.82e-32 112
MsG0380017402.01.T01 AT4G12500 38.764 178 60 4 1 130 1 177 1.91e-31 110
MsG0380017402.01.T01 AT4G12480 60.920 87 32 2 45 130 83 168 5.47e-31 108
MsG0380017402.01.T01 AT4G12470 58.889 90 35 2 42 130 73 161 8.58e-31 108
MsG0380017402.01.T01 AT5G46900 63.736 91 29 2 40 130 41 127 3.30e-30 105
MsG0380017402.01.T01 AT1G62510 61.176 85 31 2 46 129 66 149 1.61e-29 104
MsG0380017402.01.T01 AT5G46890 61.053 95 33 3 36 130 37 127 1.23e-28 101
MsG0380017402.01.T01 AT1G12090 62.195 82 29 2 49 129 57 137 1.35e-28 102
MsG0380017402.01.T01 AT4G12520 52.893 121 53 3 11 130 12 129 4.95e-28 100
MsG0380017402.01.T01 AT4G12510 52.893 121 53 3 11 130 12 129 4.95e-28 100
MsG0380017402.01.T01 AT4G12550 50.000 92 44 2 40 130 21 111 4.68e-26 94.7
MsG0380017402.01.T01 AT4G00165 47.664 107 51 3 27 129 23 128 5.64e-26 95.1
MsG0380017402.01.T01 AT4G00165 47.664 107 51 3 27 129 23 128 5.64e-26 95.1
MsG0380017402.01.T01 AT1G12100 41.129 124 70 3 9 130 10 132 9.30e-26 94.7
MsG0380017402.01.T01 AT4G22460 48.571 105 49 2 28 128 28 131 2.23e-25 93.6
MsG0380017402.01.T01 AT4G12530 50.000 86 42 1 44 129 31 115 5.50e-23 87.0
MsG0380017402.01.T01 AT4G12545 50.000 92 41 3 40 130 21 108 6.24e-23 86.7
MsG0380017402.01.T01 AT3G22142 52.381 84 37 3 47 129 1397 1478 2.11e-20 86.3
MsG0380017402.01.T01 AT3G22120 51.190 84 39 2 47 129 267 349 5.91e-19 81.3
MsG0380017402.01.T01 AT3G22120 51.190 84 39 2 47 129 250 332 1.22e-18 80.1
MsG0380017402.01.T01 AT2G10940 47.727 88 43 3 44 130 205 290 1.28e-18 79.7
MsG0380017402.01.T01 AT2G10940 47.727 88 43 3 44 130 205 290 1.28e-18 79.7
MsG0380017402.01.T01 AT4G15160 49.412 85 40 3 47 129 99 182 4.48e-17 73.9
MsG0380017402.01.T01 AT4G15160 49.412 85 40 3 47 129 181 264 1.15e-16 74.3
MsG0380017402.01.T01 AT1G62500 42.529 87 47 3 44 129 209 293 6.75e-15 69.7
MsG0380017402.01.T01 AT4G22490 37.634 93 54 3 38 129 27 116 8.29e-15 66.2

Find 44 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTAACTTTAAGGTATCTT+TGG 0.166346 3:-95856622 None:intergenic
AGCACAAACCCCTAACTTTA+AGG 0.205248 3:-95856631 None:intergenic
ATCTTTGGGGCAGTGACCTT+GGG 0.214560 3:-95856607 None:intergenic
TTGTCTTTGCACTGCAATTA+AGG 0.231170 3:+95856752 MsG0380017402.01.T01:CDS
AATCAAAGGTTTGGCTGATT+TGG 0.237754 3:+95856719 MsG0380017402.01.T01:CDS
TAAAATGGTGGCAAAGAATT+TGG 0.290687 3:-95856493 None:intergenic
CCTAACTTTAAGGTATCTTT+GGG 0.293316 3:-95856621 None:intergenic
GAAGGTGTTGGCTTTGGCTT+TGG 0.296299 3:-95856582 None:intergenic
TGGGGTGAAGGTGTTGGCTT+TGG 0.321997 3:-95856588 None:intergenic
CCCAAAGATACCTTAAAGTT+AGG 0.331524 3:+95856621 MsG0380017402.01.T01:CDS
GTTGGCTTTGGCTTTGGCTT+TGG 0.335137 3:-95856576 None:intergenic
TATCTTTGGGGCAGTGACCT+TGG 0.380498 3:-95856608 None:intergenic
CCAAAGATACCTTAAAGTTA+GGG 0.385359 3:+95856622 MsG0380017402.01.T01:CDS
AGGATCCAGGTGAGGGTGAT+AGG 0.388310 3:-95856804 None:intergenic
GGGCAGTGACCTTGGGGTGA+AGG 0.389809 3:-95856600 None:intergenic
AAGGAGAGAGAGTACTAAAA+TGG 0.390388 3:-95856508 None:intergenic
ATCACCCTCACCTGGATCCT+TGG 0.406696 3:+95856807 MsG0380017402.01.T01:CDS
GTTGTTGGGAATCCTGCTTC+AGG 0.413980 3:+95856678 MsG0380017402.01.T01:CDS
TTTGGCTTTGGCTTTGGTGA+TGG 0.416452 3:-95856570 None:intergenic
CTCAACATTTACTGAGGCTA+AGG 0.420857 3:+95856536 MsG0380017402.01.T01:CDS
TTGACAAGCACCAAGGATCC+AGG 0.457767 3:-95856817 None:intergenic
GACTGGTTAATGTTGTTGTT+GGG 0.463209 3:+95856664 MsG0380017402.01.T01:CDS
TTGTGCAATAATCAAAGGTT+TGG 0.466120 3:+95856710 MsG0380017402.01.T01:CDS
GGACTGGTTAATGTTGTTGT+TGG 0.480624 3:+95856663 MsG0380017402.01.T01:CDS
TGACCTTGGGGTGAAGGTGT+TGG 0.502306 3:-95856594 None:intergenic
AATTGTTGTGCAATAATCAA+AGG 0.523157 3:+95856705 MsG0380017402.01.T01:CDS
GCAATTAAGGCCAATGTGCT+CGG 0.533371 3:+95856765 MsG0380017402.01.T01:CDS
TCTTTGGGGCAGTGACCTTG+GGG 0.545751 3:-95856606 None:intergenic
CCTTAGGCACATTGAAAACC+AGG 0.555069 3:-95856852 None:intergenic
CAAAGATACCTTAAAGTTAG+GGG 0.560461 3:+95856623 MsG0380017402.01.T01:CDS
ATGTACCTATCACCCTCACC+TGG 0.591606 3:+95856799 MsG0380017402.01.T01:CDS
CAGTAAATGTTGAGTAAGCA+AGG 0.592270 3:-95856527 None:intergenic
TCAACATTTACTGAGGCTAA+GGG 0.596026 3:+95856537 MsG0380017402.01.T01:CDS
CAAGTTTATTCCGAGCACAT+TGG 0.596849 3:-95856775 None:intergenic
GGTGGCAAAGAATTTGGTGT+TGG 0.599992 3:-95856487 None:intergenic
CAACAATTACTACCTGAAGC+AGG 0.602543 3:-95856690 None:intergenic
AGCACCAAGGATCCAGGTGA+GGG 0.612333 3:-95856811 None:intergenic
AAGCCAACACCTTCACCCCA+AGG 0.614009 3:+95856591 MsG0380017402.01.T01:CDS
TGCTTACTCAACATTTACTG+AGG 0.622636 3:+95856530 MsG0380017402.01.T01:CDS
GAGAGAGAGTACTAAAATGG+TGG 0.630559 3:-95856505 None:intergenic
AAGCACCAAGGATCCAGGTG+AGG 0.645068 3:-95856812 None:intergenic
AGGCACATTGAAAACCAGGA+GGG 0.674973 3:-95856848 None:intergenic
CTAACTTTAAGGTATCTTTG+GGG 0.708820 3:-95856620 None:intergenic
TAGGCACATTGAAAACCAGG+AGG 0.727521 3:-95856849 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTGTTGTGCAATAATCAA+AGG + Chr3:95856705-95856724 MsG0380017402.01.T01:CDS 25.0%
CAAAGATACCTTAAAGTTAG+GGG + Chr3:95856623-95856642 MsG0380017402.01.T01:CDS 30.0%
CCAAAGATACCTTAAAGTTA+GGG + Chr3:95856622-95856641 MsG0380017402.01.T01:CDS 30.0%
TAAAATGGTGGCAAAGAATT+TGG - Chr3:95856496-95856515 None:intergenic 30.0%
TTGTGCAATAATCAAAGGTT+TGG + Chr3:95856710-95856729 MsG0380017402.01.T01:CDS 30.0%
!! CCTAACTTTAAGGTATCTTT+GGG - Chr3:95856624-95856643 None:intergenic 30.0%
!! CTAACTTTAAGGTATCTTTG+GGG - Chr3:95856623-95856642 None:intergenic 30.0%
AATCAAAGGTTTGGCTGATT+TGG + Chr3:95856719-95856738 MsG0380017402.01.T01:CDS 35.0%
CAGTAAATGTTGAGTAAGCA+AGG - Chr3:95856530-95856549 None:intergenic 35.0%
CCCAAAGATACCTTAAAGTT+AGG + Chr3:95856621-95856640 MsG0380017402.01.T01:CDS 35.0%
TCAACATTTACTGAGGCTAA+GGG + Chr3:95856537-95856556 MsG0380017402.01.T01:CDS 35.0%
TGCTTACTCAACATTTACTG+AGG + Chr3:95856530-95856549 MsG0380017402.01.T01:CDS 35.0%
TTGTCTTTGCACTGCAATTA+AGG + Chr3:95856752-95856771 MsG0380017402.01.T01:CDS 35.0%
! GACTGGTTAATGTTGTTGTT+GGG + Chr3:95856664-95856683 MsG0380017402.01.T01:CDS 35.0%
!! AAGGAGAGAGAGTACTAAAA+TGG - Chr3:95856511-95856530 None:intergenic 35.0%
!! CCCTAACTTTAAGGTATCTT+TGG - Chr3:95856625-95856644 None:intergenic 35.0%
!!! TTGTTTTTTGACAAGCACCA+AGG - Chr3:95856827-95856846 None:intergenic 35.0%
AGCACAAACCCCTAACTTTA+AGG - Chr3:95856634-95856653 None:intergenic 40.0%
CAACAATTACTACCTGAAGC+AGG - Chr3:95856693-95856712 None:intergenic 40.0%
CAAGTTTATTCCGAGCACAT+TGG - Chr3:95856778-95856797 None:intergenic 40.0%
CTCAACATTTACTGAGGCTA+AGG + Chr3:95856536-95856555 MsG0380017402.01.T01:CDS 40.0%
TGTCAAAAAACAATCCCTCC+TGG + Chr3:95856834-95856853 MsG0380017402.01.T01:CDS 40.0%
!! GAGAGAGAGTACTAAAATGG+TGG - Chr3:95856508-95856527 None:intergenic 40.0%
!! GGACTGGTTAATGTTGTTGT+TGG + Chr3:95856663-95856682 MsG0380017402.01.T01:CDS 40.0%
!!! TTGTGCTGATGTTTTAGGAC+TGG + Chr3:95856647-95856666 MsG0380017402.01.T01:CDS 40.0%
AGGCACATTGAAAACCAGGA+GGG - Chr3:95856851-95856870 None:intergenic 45.0%
GCAATTAAGGCCAATGTGCT+CGG + Chr3:95856765-95856784 MsG0380017402.01.T01:CDS 45.0%
TAGGCACATTGAAAACCAGG+AGG - Chr3:95856852-95856871 None:intergenic 45.0%
!! GGGGTTTGTGCTGATGTTTT+AGG + Chr3:95856642-95856661 MsG0380017402.01.T01:CDS 45.0%
!! GGTGGCAAAGAATTTGGTGT+TGG - Chr3:95856490-95856509 None:intergenic 45.0%
!! TTTGGCTTTGGCTTTGGTGA+TGG - Chr3:95856573-95856592 None:intergenic 45.0%
ATGTACCTATCACCCTCACC+TGG + Chr3:95856799-95856818 MsG0380017402.01.T01:CDS 50.0%
GTTGTTGGGAATCCTGCTTC+AGG + Chr3:95856678-95856697 MsG0380017402.01.T01:CDS 50.0%
TTGACAAGCACCAAGGATCC+AGG - Chr3:95856820-95856839 None:intergenic 50.0%
!! ATCTTTGGGGCAGTGACCTT+GGG - Chr3:95856610-95856629 None:intergenic 50.0%
!! GAAGGTGTTGGCTTTGGCTT+TGG - Chr3:95856585-95856604 None:intergenic 50.0%
!! GTTGGCTTTGGCTTTGGCTT+TGG - Chr3:95856579-95856598 None:intergenic 50.0%
!! TATCTTTGGGGCAGTGACCT+TGG - Chr3:95856611-95856630 None:intergenic 50.0%
AAGCACCAAGGATCCAGGTG+AGG - Chr3:95856815-95856834 None:intergenic 55.0%
AAGCCAACACCTTCACCCCA+AGG + Chr3:95856591-95856610 MsG0380017402.01.T01:CDS 55.0%
AGCACCAAGGATCCAGGTGA+GGG - Chr3:95856814-95856833 None:intergenic 55.0%
AGGATCCAGGTGAGGGTGAT+AGG - Chr3:95856807-95856826 None:intergenic 55.0%
ATCACCCTCACCTGGATCCT+TGG + Chr3:95856807-95856826 MsG0380017402.01.T01:CDS 55.0%
! TGACCTTGGGGTGAAGGTGT+TGG - Chr3:95856597-95856616 None:intergenic 55.0%
!! TCTTTGGGGCAGTGACCTTG+GGG - Chr3:95856609-95856628 None:intergenic 55.0%
!! TGGGGTGAAGGTGTTGGCTT+TGG - Chr3:95856591-95856610 None:intergenic 55.0%
GGGCAGTGACCTTGGGGTGA+AGG - Chr3:95856603-95856622 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 95856480 95856872 95856480 ID=MsG0380017402.01;Name=MsG0380017402.01
Chr3 mRNA 95856480 95856872 95856480 ID=MsG0380017402.01.T01;Parent=MsG0380017402.01;Name=MsG0380017402.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|130
Chr3 exon 95856480 95856872 95856480 ID=MsG0380017402.01.T01:exon:4760;Parent=MsG0380017402.01.T01
Chr3 CDS 95856480 95856872 95856480 ID=MsG0380017402.01.T01:cds;Parent=MsG0380017402.01.T01
Gene Sequence

>MsG0380017402.01.T01

ATGGCTTCCAACACCAAATTCTTTGCCACCATTTTAGTACTCTCTCTCCTTGCTTACTCAACATTTACTGAGGCTAAGGGTTCATGCTCACCATCACCAAAGCCAAAGCCAAAGCCAACACCTTCACCCCAAGGTCACTGCCCCAAAGATACCTTAAAGTTAGGGGTTTGTGCTGATGTTTTAGGACTGGTTAATGTTGTTGTTGGGAATCCTGCTTCAGGTAGTAATTGTTGTGCAATAATCAAAGGTTTGGCTGATTTGGATGCTGCACTTTGTCTTTGCACTGCAATTAAGGCCAATGTGCTCGGAATAAACTTGAATGTACCTATCACCCTCACCTGGATCCTTGGTGCTTGTCAAAAAACAATCCCTCCTGGTTTTCAATGTGCCTAA

Protein sequence

>MsG0380017402.01.T01

MASNTKFFATILVLSLLAYSTFTEAKGSCSPSPKPKPKPTPSPQGHCPKDTLKLGVCADVLGLVNVVVGNPASGSNCCAIIKGLADLDAALCLCTAIKANVLGINLNVPITLTWILGACQKTIPPGFQCA*