AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048611.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048611.01.T01 MTR_7g094710 100.000 47 0 0 1 47 1 47 3.80e-28 96.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048611.01.T01 AT3G52420 65.455 55 16 1 1 52 1 55 1.40e-17 69.3

Find 23 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAAGCGGTTGTGGTTGC+TGG 0.323695 contig374end:-14749 MsG0080048611.01.T01:CDS
CTGGAGCCTTAGCATTTGTC+TGG 0.357220 contig374end:-14730 MsG0080048611.01.T01:CDS
GCATCTCCGGTATCAGCTTC+AGG 0.383647 contig374end:+14602 None:intergenic
GCTTCAGGAGCGGCGGCAGC+AGG 0.386627 contig374end:+14617 None:intergenic
TGCAGTTGCGGTGGCATCTC+CGG 0.432441 contig374end:+14589 None:intergenic
GCGGCAGCAGGAACATCATC+AGG 0.447838 contig374end:+14629 None:intergenic
TAGAGTCGCGGGTCTGAGAA+AGG 0.465845 contig374end:+14678 None:intergenic
GCCGCTCCTGAAGCTGATAC+CGG 0.517196 contig374end:-14608 MsG0080048611.01.T01:CDS
CATCATCAGGATCACGGGTC+GGG 0.517406 contig374end:+14642 None:intergenic
AAATTAGGCGTCTGCAGTTG+CGG 0.528752 contig374end:+14577 None:intergenic
GTCGCGGGTCTGAGAAAGGA+AGG 0.546452 contig374end:+14682 None:intergenic
AGGAACATCATCAGGATCAC+GGG 0.573103 contig374end:+14637 None:intergenic
AAAGGCAAAAGAAGCGGTTG+TGG 0.575609 contig374end:-14756 MsG0080048611.01.T01:CDS
CAGGAACATCATCAGGATCA+CGG 0.582934 contig374end:+14636 None:intergenic
AGCGAGCCAGACAAATGCTA+AGG 0.593697 contig374end:+14724 None:intergenic
AGATTTGTCGATAGAGTCGC+GGG 0.604367 contig374end:+14667 None:intergenic
ACATCATCAGGATCACGGGT+CGG 0.609823 contig374end:+14641 None:intergenic
CAGATTTGTCGATAGAGTCG+CGG 0.623137 contig374end:+14666 None:intergenic
TTAGGCGTCTGCAGTTGCGG+TGG 0.639439 contig374end:+14580 None:intergenic
TCCGGTATCAGCTTCAGGAG+CGG 0.639504 contig374end:+14607 None:intergenic
GGTATCAGCTTCAGGAGCGG+CGG 0.662347 contig374end:+14610 None:intergenic
GATGGGAAAGGCAAAAGAAG+CGG 0.676214 contig374end:-14762 MsG0080048611.01.T01:CDS
TCGCGGGTCTGAGAAAGGAA+GGG 0.692341 contig374end:+14683 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGGCAAAAGAAGCGGTTG+TGG - contig374end:14585-14604 MsG0080048611.01.T01:CDS 45.0%
AGATTTGTCGATAGAGTCGC+GGG + contig374end:14677-14696 None:intergenic 45.0%
AGGAACATCATCAGGATCAC+GGG + contig374end:14707-14726 None:intergenic 45.0%
CAGATTTGTCGATAGAGTCG+CGG + contig374end:14678-14697 None:intergenic 45.0%
CAGGAACATCATCAGGATCA+CGG + contig374end:14708-14727 None:intergenic 45.0%
AAAGAAGCGGTTGTGGTTGC+TGG - contig374end:14592-14611 MsG0080048611.01.T01:CDS 50.0%
ACATCATCAGGATCACGGGT+CGG + contig374end:14703-14722 None:intergenic 50.0%
AGCGAGCCAGACAAATGCTA+AGG + contig374end:14620-14639 None:intergenic 50.0%
CTGGAGCCTTAGCATTTGTC+TGG - contig374end:14611-14630 MsG0080048611.01.T01:CDS 50.0%
CATCATCAGGATCACGGGTC+GGG + contig374end:14702-14721 None:intergenic 55.0%
GCATCTCCGGTATCAGCTTC+AGG + contig374end:14742-14761 None:intergenic 55.0%
TAGAGTCGCGGGTCTGAGAA+AGG + contig374end:14666-14685 None:intergenic 55.0%
TCCGGTATCAGCTTCAGGAG+CGG + contig374end:14737-14756 None:intergenic 55.0%
TCGCGGGTCTGAGAAAGGAA+GGG + contig374end:14661-14680 None:intergenic 55.0%
GCCGCTCCTGAAGCTGATAC+CGG - contig374end:14733-14752 MsG0080048611.01.T01:CDS 60.0%
GCGGCAGCAGGAACATCATC+AGG + contig374end:14715-14734 None:intergenic 60.0%
GGTATCAGCTTCAGGAGCGG+CGG + contig374end:14734-14753 None:intergenic 60.0%
GTCGCGGGTCTGAGAAAGGA+AGG + contig374end:14662-14681 None:intergenic 60.0%
TGCAGTTGCGGTGGCATCTC+CGG + contig374end:14755-14774 None:intergenic 60.0%
TTAGGCGTCTGCAGTTGCGG+TGG + contig374end:14764-14783 None:intergenic 60.0%
GCTTCAGGAGCGGCGGCAGC+AGG + contig374end:14727-14746 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
contig374end gene 14580 14783 14580 ID=MsG0080048611.01;Name=MsG0080048611.01
contig374end mRNA 14580 14783 14580 ID=MsG0080048611.01.T01;Parent=MsG0080048611.01;Name=MsG0080048611.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|67
contig374end exon 14580 14783 14580 ID=MsG0080048611.01.T01:exon:18355;Parent=MsG0080048611.01.T01
contig374end CDS 14580 14783 14580 ID=MsG0080048611.01.T01:cds;Parent=MsG0080048611.01.T01
Gene Sequence

>MsG0080048611.01.T01

ATGGGAAAGGCAAAAGAAGCGGTTGTGGTTGCTGGAGCCTTAGCATTTGTCTGGCTCGCTATTGAACTCGCTTTCAAACCCTTCCTTTCTCAGACCCGCGACTCTATCGACAAATCTGACCCGACCCGTGATCCTGATGATGTTCCTGCTGCCGCCGCTCCTGAAGCTGATACCGGAGATGCCACCGCAACTGCAGACGCCTAA

Protein sequence

>MsG0080048611.01.T01

MGKAKEAVVVAGALAFVWLAIELAFKPFLSQTRDSIDKSDPTRDPDDVPAAAAPEADTGDATATADA*