Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024597.01.T01 | XP_003611408.1 | 97.606 | 543 | 13 | 0 | 1 | 543 | 1 | 543 | 0 | 1119 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048253.01 | MsG0580024597.01 | -0.802004 | 6.890568e-49 | 3.338189e-46 |
| MsG0080049027.01 | MsG0580024597.01 | -0.810259 | 1.259756e-50 | 7.558006e-48 |
| MsG0180000151.01 | MsG0580024597.01 | 0.838371 | 2.992926e-57 | 4.000412e-54 |
| MsG0180004025.01 | MsG0580024597.01 | 0.808863 | 2.512964e-50 | 1.452917e-47 |
| MsG0180005339.01 | MsG0580024597.01 | 0.813594 | 2.365125e-51 | 1.551119e-48 |
| MsG0280006669.01 | MsG0580024597.01 | 0.804131 | 2.503092e-49 | 1.280447e-46 |
| MsG0280007429.01 | MsG0580024597.01 | 0.894220 | 3.026389e-75 | 2.981469e-71 |
| MsG0280011073.01 | MsG0580024597.01 | 0.835277 | 1.842944e-56 | 2.242988e-53 |
| MsG0280011287.01 | MsG0580024597.01 | 0.831181 | 1.930983e-55 | 2.079386e-52 |
| MsG0380012184.01 | MsG0580024597.01 | 0.804488 | 2.109019e-49 | 1.088782e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024597.01.T01 | MTR_5g013620 | 97.606 | 543 | 13 | 0 | 1 | 543 | 1 | 543 | 0.0 | 1119 |
| MsG0580024597.01.T01 | MTR_5g013640 | 91.376 | 545 | 43 | 2 | 1 | 543 | 1 | 543 | 0.0 | 1040 |
| MsG0580024597.01.T01 | MTR_4g099110 | 73.616 | 542 | 125 | 5 | 1 | 535 | 1 | 531 | 0.0 | 832 |
| MsG0580024597.01.T01 | MTR_6g477780 | 62.865 | 342 | 115 | 2 | 181 | 510 | 10 | 351 | 3.55e-165 | 474 |
| MsG0580024597.01.T01 | MTR_4g045667 | 49.655 | 435 | 202 | 4 | 87 | 507 | 11 | 442 | 1.06e-155 | 454 |
| MsG0580024597.01.T01 | MTR_2g089030 | 49.771 | 436 | 202 | 4 | 86 | 507 | 83 | 515 | 6.41e-149 | 440 |
| MsG0580024597.01.T01 | MTR_8g099775 | 46.039 | 467 | 231 | 7 | 90 | 543 | 80 | 538 | 6.02e-140 | 416 |
| MsG0580024597.01.T01 | MTR_7g077410 | 46.275 | 443 | 221 | 6 | 86 | 515 | 262 | 700 | 5.04e-123 | 377 |
| MsG0580024597.01.T01 | MTR_2g020240 | 38.137 | 451 | 269 | 4 | 63 | 508 | 57 | 502 | 7.98e-105 | 325 |
| MsG0580024597.01.T01 | MTR_5g026210 | 37.797 | 463 | 270 | 8 | 59 | 510 | 189 | 644 | 4.21e-103 | 324 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024597.01.T01 | AT4G17090 | 68.051 | 554 | 160 | 8 | 1 | 543 | 1 | 548 | 0.0 | 784 |
| MsG0580024597.01.T01 | AT3G23920 | 65.138 | 436 | 138 | 2 | 89 | 510 | 106 | 541 | 0.0 | 613 |
| MsG0580024597.01.T01 | AT4G15210 | 48.394 | 436 | 207 | 5 | 87 | 507 | 12 | 444 | 6.89e-149 | 437 |
| MsG0580024597.01.T01 | AT4G15210 | 51.795 | 390 | 183 | 4 | 87 | 474 | 12 | 398 | 1.49e-146 | 428 |
| MsG0580024597.01.T01 | AT2G32290 | 44.979 | 478 | 245 | 6 | 43 | 507 | 34 | 506 | 5.99e-141 | 419 |
| MsG0580024597.01.T01 | AT4G00490 | 48.843 | 432 | 204 | 6 | 89 | 507 | 95 | 522 | 1.09e-138 | 412 |
| MsG0580024597.01.T01 | AT4G15210 | 47.608 | 418 | 195 | 5 | 128 | 531 | 1 | 408 | 1.22e-138 | 409 |
| MsG0580024597.01.T01 | AT4G15210 | 47.608 | 418 | 195 | 5 | 128 | 531 | 1 | 408 | 1.22e-138 | 409 |
| MsG0580024597.01.T01 | AT5G55700 | 43.964 | 439 | 227 | 5 | 88 | 508 | 91 | 528 | 2.29e-130 | 390 |
| MsG0580024597.01.T01 | AT2G45880 | 46.387 | 429 | 213 | 6 | 89 | 504 | 248 | 672 | 5.02e-122 | 374 |
Find 138 sgRNAs with CRISPR-Local
Find 184 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCATATTCCAAACAAG+TGG | 0.558992 | 5:-6732618 | MsG0580024597.01.T01:CDS |
| AAACTATCTGCCAAAATAGC+TGG | 0.456022 | 5:-6732591 | MsG0580024597.01.T01:CDS |
| AAATCCTGAATATATCTCAC+TGG | 0.483131 | 5:-6734029 | MsG0580024597.01.T01:CDS |
| AACACATATATCATGTTGAT+GGG | 0.560154 | 5:-6732060 | MsG0580024597.01.T01:three_prime_UTR |
| AAGAAAGAGTGGGGAACAAG+TGG | 0.590788 | 5:-6732792 | MsG0580024597.01.T01:CDS |
| AAGCATCAGCAGGGGCAATT+GGG | 0.462242 | 5:-6732815 | MsG0580024597.01.T01:CDS |
| AAGGAGTAATGGTTGATGCT+TGG | 0.565190 | 5:-6734431 | MsG0580024597.01.T01:CDS |
| AAGTGGGACTAGGTCCTTGT+GGG | 0.419249 | 5:-6733471 | MsG0580024597.01.T01:CDS |
| AATAAATAAGAGGTTGCTTG+AGG | 0.517623 | 5:-6732241 | MsG0580024597.01.T01:CDS |
| AATAATCTGTAAATCTATCG+CGG | 0.635987 | 5:+6733936 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATCCTGAATATATCTCAC+TGG | - | Chr5:6732688-6732707 | MsG0580024597.01.T01:CDS | 30.0% | |
| AATAAATAAGAGGTTGCTTG+AGG | - | Chr5:6734476-6734495 | MsG0580024597.01.T01:CDS | 30.0% | |
| AATCCTGAATATATCTCACT+GGG | - | Chr5:6732689-6732708 | MsG0580024597.01.T01:CDS | 30.0% | |
| AGATATGATTCAAGTGCTTA+TGG | - | Chr5:6734408-6734427 | MsG0580024597.01.T01:CDS | 30.0% | |
| ATAAATAAGAGGTTGCTTGA+GGG | - | Chr5:6734477-6734496 | MsG0580024597.01.T01:CDS | 30.0% | |
| ATTGGTTAATCAAGTGAGAA+TGG | - | Chr5:6734338-6734357 | MsG0580024597.01.T01:CDS | 30.0% | |
| CTGTAAAGAAAGACAAGAAA+TGG | - | Chr5:6732100-6732119 | MsG0580024597.01.T01:CDS | 30.0% | |
| GAACACATATATCATGTTGA+TGG | - | Chr5:6734656-6734675 | MsG0580024597.01.T01:CDS | 30.0% | |
| GAACTTCCTGCAAATTAATT+TGG | + | Chr5:6733226-6733245 | None:intergenic | 30.0% | |
| GCAAATAGAAATAACACACA+AGG | - | Chr5:6731857-6731876 | MsG0580024597.01.T01:three_prime_UTR | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 6731857 | 6734882 | 6731857 | ID=MsG0580024597.01;Name=MsG0580024597.01 |
| Chr5 | mRNA | 6731857 | 6734882 | 6731857 | ID=MsG0580024597.01.T01;Parent=MsG0580024597.01;Name=MsG0580024597.01.T01;_AED=0.34;_eAED=0.34;_QI=56|1|1|1|1|1|4|232|543 |
| Chr5 | exon | 6731857 | 6732856 | 6731857 | ID=MsG0580024597.01.T01:exon:25862;Parent=MsG0580024597.01.T01 |
| Chr5 | exon | 6733390 | 6733500 | 6733390 | ID=MsG0580024597.01.T01:exon:25861;Parent=MsG0580024597.01.T01 |
| Chr5 | exon | 6733919 | 6734129 | 6733919 | ID=MsG0580024597.01.T01:exon:25860;Parent=MsG0580024597.01.T01 |
| Chr5 | exon | 6734285 | 6734882 | 6734285 | ID=MsG0580024597.01.T01:exon:25859;Parent=MsG0580024597.01.T01 |
| Chr5 | five_prime_UTR | 6734827 | 6734882 | 6734827 | ID=MsG0580024597.01.T01:five_prime_utr;Parent=MsG0580024597.01.T01 |
| Chr5 | CDS | 6734285 | 6734826 | 6734285 | ID=MsG0580024597.01.T01:cds;Parent=MsG0580024597.01.T01 |
| Chr5 | CDS | 6733919 | 6734129 | 6733919 | ID=MsG0580024597.01.T01:cds;Parent=MsG0580024597.01.T01 |
| Chr5 | CDS | 6733390 | 6733500 | 6733390 | ID=MsG0580024597.01.T01:cds;Parent=MsG0580024597.01.T01 |
| Chr5 | CDS | 6732089 | 6732856 | 6732089 | ID=MsG0580024597.01.T01:cds;Parent=MsG0580024597.01.T01 |
| Chr5 | three_prime_UTR | 6731857 | 6732088 | 6731857 | ID=MsG0580024597.01.T01:three_prime_utr;Parent=MsG0580024597.01.T01 |
| Gene Sequence |
>MsG0580024597.01.T01 ATGGCTCAAGCACTTGGTTTCTCAATTTCCTTGATTAATCAGAAAGAAACAAGAGTCTTAAAATCTCTTGATGATTTTTTATCCAACAAAGTTTCTTGTCCAAAAATTAAACCATCTTTTGGTCACAAACTGAAAGCAAAGAGTTCAATGCAAGAAACACATTTCACAACAGACAACAACAACTCTGCTGTAAAGAAAGACAAGAAATGGGAGAAGATTCATACGTCATCTGTCACTCATAACCACGATGGTGATTCGAATAGAGTACCGGTGTTTGTGATGTTGCCATTAGACACGGTAACAATGGGAGGAAAATTGAATAAAGCAAGAGCAATGAATGCTAGTTTGATGGCATTGAAGAGTGCTGGAGTTGAAGGAGTAATGGTTGATGCTTGGTGGGGTTTGGTTGAAAAAGATGGTCCTATGAATTATAATTGGGAAGCTTATGCTGAGCTTGTTCAGATGGTACAAAAACATGGTTTGAAGCTTCAAATTGTTATGTCTTTTCATCAATGTGGTGGAAATGTTGGTGACTCTTGCAGCATTCCTCTACCTCCATGGGTGATGGAAGAAATCAGGAAGAACCCTGAGTTGGTTTACACAGACAAATTAGGGAGGAGAAATCCTGAATATATCTCACTGGGATGTGATTCAGTGCCTGTTCTAGCAGGAAGAACTCCACTTCAGGTCTACTCCGATTACATGAGAAGCTTCCGCGATAGATTTACAGATTATTTGGGCAATGTTATCATAGAAGTTCAAGTGGGACTAGGTCCTTGTGGGGAGCTGAGATATCCATCTTACCCTGAAACTGATGGAACTTGGAAGTTTCCCGGAATTGGAGAATTCCAATGCTATGACAAGTATATGAGATCATCCTTGGAAGCATCAGCAGGGGCAATTGGGAAGAAAGAGTGGGGAACAAGTGGACCTCATGATTCTGGTCAATACAATCAATTTCCTGAGGATACTGGATTTTTCAAAAGAGAAGGAACATGGAACACAGAATATGGAGATTTCTTCCTTGATTGGTACTCAAGCAAACTTGTTGAACATGGTGAGAAAATCCTTGTATCAGCCAAAAGCATATTCCAAACAAGTGGAGTGAAACTATCTGCCAAAATAGCTGGAATTCATTGGCATTACAATGCAAGATCACATGCAACTGAATTAACTGCAGGTTACTATAACACAAGGTTTCATGATGGTTATATACCAATAGCACAAATGCTAGCAAAACACGGAGTTATATTGAATTTCACATGTATGGAAATGAAGGATAATGAACAACCTTGTGATGCTAATTGTTCACCAGAAGGATTGGTTAATCAAGTGAGAATGGCAACAAAGATGGCAGGTGGTGAACTTGCTGGTGAGAATGCATTAGAGAGATATGATTCAAGTGCTTATGGTCAAGTTTTGTCTACAAGTGGATTAAGTGCTTTTACTTATTTAAGAATAAATAAGAGGTTGCTTGAGGGTGATAATTGGAGGAAATTTGTGGATTTTGTTGTGAGTATGTCTGATGGTGGTAAACTAAGGCTTGCTGAAGCTGATTCATATGGGACTGATCTTTATGTTGGACACATAATTAAGGAGAATGATGTTATAATAGAGGTTGCTCTTGTGTGA |
| Protein sequence |
>MsG0580024597.01.T01 MAQALGFSISLINQKETRVLKSLDDFLSNKVSCPKIKPSFGHKLKAKSSMQETHFTTDNNNSAVKKDKKWEKIHTSSVTHNHDGDSNRVPVFVMLPLDTVTMGGKLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMNYNWEAYAELVQMVQKHGLKLQIVMSFHQCGGNVGDSCSIPLPPWVMEEIRKNPELVYTDKLGRRNPEYISLGCDSVPVLAGRTPLQVYSDYMRSFRDRFTDYLGNVIIEVQVGLGPCGELRYPSYPETDGTWKFPGIGEFQCYDKYMRSSLEASAGAIGKKEWGTSGPHDSGQYNQFPEDTGFFKREGTWNTEYGDFFLDWYSSKLVEHGEKILVSAKSIFQTSGVKLSAKIAGIHWHYNARSHATELTAGYYNTRFHDGYIPIAQMLAKHGVILNFTCMEMKDNEQPCDANCSPEGLVNQVRMATKMAGGELAGENALERYDSSAYGQVLSTSGLSAFTYLRINKRLLEGDNWRKFVDFVVSMSDGGKLRLAEADSYGTDLYVGHIIKENDVIIEVALV* |
