AlfalfaGEDB | MsG0380017944.01

M. sativa cultivar ZhongmuNo.1 / MsG0380017944.01

KEGG: K05894
GO: GO:0003824 GO:0010181 GO:0016491 GO:0055114
PFAM: PF00724

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017944.01.T01	XP_017436303.1	78.413	315	68	0	1	315	27	341	0	528

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017944.01.T01	Q69TH8	53.652	356	110	3	14	314	15	370	2.59E-130	379

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017944.01.T01	A0A2Z6NK81	71.625	363	49	2	9	317	9	371	0.0	516

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000367.01	MsG0380017944.01	0.812217	4.737669e-51	2.994534e-48
MsG0180003484.01	MsG0380017944.01	0.808935	2.425247e-50	1.404847e-47
MsG0380017944.01	MsG0480023322.01	0.810727	9.982099e-51	6.063847e-48
MsG0380017944.01	MsG0580026492.01	0.812568	3.971375e-51	2.533726e-48
MsG0380017944.01	MsG0580028302.01	0.808376	3.192922e-50	1.822725e-47
MsG0380017944.01	MsG0880047450.01	0.806937	6.454225e-50	3.548354e-47
MsG0380017944.01	MsG0880047664.01	0.806044	9.960138e-50	5.352027e-47
MsG0380017944.01	MsG0880047714.01	0.802245	6.148187e-49	2.996946e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017944.01.T01	MTR_3g115600	64.835	364	73	6	9	317	3	366	1.22e-151	430
MsG0380017944.01.T01	MTR_5g006670	57.062	354	99	1	14	314	6	359	9.58e-143	407
MsG0380017944.01.T01	MTR_5g006710	55.367	354	105	5	14	314	10	363	2.24e-133	384
MsG0380017944.01.T01	MTR_5g006740	53.936	343	116	2	14	314	6	348	4.16e-130	375
MsG0380017944.01.T01	MTR_5g006740	50.556	360	119	4	14	314	6	365	4.19e-123	357
MsG0380017944.01.T01	MTR_5g006780	46.832	363	130	6	14	314	10	371	1.02e-99	298
MsG0380017944.01.T01	MTR_5g006800	69.588	194	59	0	121	314	176	369	1.30e-98	295
MsG0380017944.01.T01	MTR_5g006800	66.964	112	37	0	8	119	4	115	1.15e-47	164
MsG0380017944.01.T01	MTR_5g008040	68.394	193	61	0	121	313	178	370	1.53e-97	293
MsG0380017944.01.T01	MTR_5g008040	59.091	110	45	0	14	123	12	121	7.67e-41	146

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017944.01.T01	AT1G09400	54.206	321	94	1	25	292	4	324	6.40e-120	348
MsG0380017944.01.T01	AT1G09400	56.061	264	63	1	82	292	2	265	7.36e-101	297
MsG0380017944.01.T01	AT1G76680	69.231	195	60	0	121	315	176	370	7.18e-97	291
MsG0380017944.01.T01	AT1G76680	69.231	195	60	0	121	315	201	395	2.23e-96	290
MsG0380017944.01.T01	AT1G76680	64.407	118	42	0	5	122	1	118	2.49e-46	161
MsG0380017944.01.T01	AT1G76690	68.205	195	62	0	121	315	178	372	1.08e-95	288
MsG0380017944.01.T01	AT1G17990	47.674	258	81	3	8	211	1	258	5.63e-78	239
MsG0380017944.01.T01	AT1G18020	47.674	258	81	3	8	211	1	258	5.63e-78	239
MsG0380017944.01.T01	AT1G17990	44.961	258	80	4	8	211	1	250	1.67e-69	217
MsG0380017944.01.T01	AT2G06050	50.242	207	89	3	121	313	179	385	6.05e-65	209

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Find 86 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAGTTGTTGATGAGAT+TGG	0.442364	3:-102500863	MsG0380017944.01.T01:CDS
AACAAAGGGTGGCTTCTTGA+TGG	0.417485	3:-102502828	MsG0380017944.01.T01:CDS
AACAAGGTCCTTCAATTTCG+TGG	0.462495	3:-102502889	MsG0380017944.01.T01:CDS
AACAATGTGTTGGCCAGCAA+TGG	0.583715	3:-102500590	MsG0380017944.01.T01:CDS
AACGACAGAGATGATGAGTA+TGG	0.548461	3:-102500929	MsG0380017944.01.T01:CDS
AAGGACCTTGTTCTTGTTAA+AGG	0.178211	3:+102502900	None:intergenic
AATCAATTATATAAACAGAG+TGG	0.615746	3:-102503160	None:intergenic
AATGGAACATTTATTGTTGC+TGG	0.345581	3:-102500635	MsG0380017944.01.T01:CDS
ACAAAGGGTGGCTTCTTGAT+GGG	0.459306	3:-102502827	MsG0380017944.01.T01:CDS
ACAGAGATGATGAGTATGGT+GGG	0.612337	3:-102500925	MsG0380017944.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACTGATGACACAAACA+AGG	+	Chr3:102501531-102501550	None:intergenic	30.0%
AAAGTCAATCAATGTGATGA+GGG	+	Chr3:102500505-102500524	None:intergenic	30.0%
AACGTTCTATCGATATATGT+AGG	+	Chr3:102502011-102502030	None:intergenic	30.0%
AATGGAACATTTATTGTTGC+TGG	-	Chr3:102502864-102502883	MsG0380017944.01.T01:CDS	30.0%
ATGAGCTTGAGAACAAAAAT+AGG	+	Chr3:102500741-102500760	None:intergenic	30.0%
ATTCTTCAACGAACAACAAA+GGG	-	Chr3:102500657-102500676	MsG0380017944.01.T01:CDS	30.0%
CATTCACAATAATCAGCATA+AGG	+	Chr3:102502690-102502709	None:intergenic	30.0%
CCGAACTTTAGATTATTTGT+TGG	-	Chr3:102501574-102501593	MsG0380017944.01.T01:intron	30.0%
CTAATAATTACCTGCTTCTA+TGG	+	Chr3:102502189-102502208	None:intergenic	30.0%
CTCTAGTGTAATCTAATTAG+AGG	+	Chr3:102502333-102502352	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	102500347	102503174	102500347	ID=MsG0380017944.01;Name=MsG0380017944.01
Chr3	mRNA	102500347	102503174	102500347	ID=MsG0380017944.01.T01;Parent=MsG0380017944.01;Name=MsG0380017944.01.T01;_AED=0.49;_eAED=0.49;_QI=39\|0.66\|1\|1\|1\|1\|4\|77\|318
Chr3	exon	102500347	102501019	102500347	ID=MsG0380017944.01.T01:exon:18280;Parent=MsG0380017944.01.T01
Chr3	exon	102502168	102502298	102502168	ID=MsG0380017944.01.T01:exon:18279;Parent=MsG0380017944.01.T01
Chr3	exon	102502795	102502935	102502795	ID=MsG0380017944.01.T01:exon:18278;Parent=MsG0380017944.01.T01
Chr3	exon	102503047	102503174	102503047	ID=MsG0380017944.01.T01:exon:18277;Parent=MsG0380017944.01.T01
Chr3	five_prime_UTR	102503136	102503174	102503136	ID=MsG0380017944.01.T01:five_prime_utr;Parent=MsG0380017944.01.T01
Chr3	CDS	102503047	102503135	102503047	ID=MsG0380017944.01.T01:cds;Parent=MsG0380017944.01.T01
Chr3	CDS	102502795	102502935	102502795	ID=MsG0380017944.01.T01:cds;Parent=MsG0380017944.01.T01
Chr3	CDS	102502168	102502298	102502168	ID=MsG0380017944.01.T01:cds;Parent=MsG0380017944.01.T01
Chr3	CDS	102500424	102501019	102500424	ID=MsG0380017944.01.T01:cds;Parent=MsG0380017944.01.T01
Chr3	three_prime_UTR	102500347	102500423	102500347	ID=MsG0380017944.01.T01:three_prime_utr;Parent=MsG0380017944.01.T01

Gene Sequence

>MsG0380017944.01.T01

ATGGAAGAGAGAATAGAAGTGGTTGAAGGAAAAGAAGTGATACCCCTTCTCACCCCCATACAACATGGGAAAATTCATCTGTCCCACAGGATAGTTCTTGCACCTTTAACAAGAACAAGGTCCTTCAATTTCGTGGCTCAACCAGCACATGCTGCTCTTTATTCTTCAACGAACAACAAAGGGTGGCTTCTTGATGGGGAAGCCTCAGGGGTCTCAGACACTGCACAAGGGTACCCAAATACACCTGGTATTTGGACAAGAGAACAGGTGGAAGCATGGAAACCCATTGTTGGAGCTGTTCATGAGAAAGGAGGCATATTCTTTTGTCAACTTTGGCACGCTGGAAGAGTGTCCAACTATGGTTTTGATGGAGTGGAGATACATGGAGCTAATGGATACTTGCTTGATCAATTCCTAAAGGACAAAGTGAACGACAGAGATGATGAGTATGGTGGGAGCGTAGAAAATCGTTGTCGCTTCCCTCTAGAGGTTGTGAAAGCAGTTGTTGATGAGATTGGAGCTGACAAAGTTGGTGTTAGACTTTCACCTTATGCTGATTATTGTGAATGTGGAGACTCTAACCCTCATGCTTTAGGCATCTACATGGCTAAGTCATTAAGTGAATTAGGCATTCTCTATTGTCATGTCATTGAACCTGGGATGTGCACCATGTTTGAAAAATGTGACACCAATGAGTCCTTAATGCCCATGAGAAAGATCTTCAATGGAACATTTATTGTTGCTGGTGGATACAATAGGACTGAAGGAAACAATGTGTTGGCCAGCAATGGTGCGGATTTGGTAGCTTATGGAAGGCTCTTTTTGGCAAATCCAGATCTTCCTAGAAGATTTGAGTTGGATGCCCAATTGAACAAGGCTGATAAGAGTACTTTTTACACCAATGATCCTGTTGTTGGATATACTGATTATCCATTTCTTGAAAATGCTACTAGCTAG

Protein sequence

>MsG0380017944.01.T01

MEERIEVVEGKEVIPLLTPIQHGKIHLSHRIVLAPLTRTRSFNFVAQPAHAALYSSTNNKGWLLDGEASGVSDTAQGYPNTPGIWTREQVEAWKPIVGAVHEKGGIFFCQLWHAGRVSNYGFDGVEIHGANGYLLDQFLKDKVNDRDDEYGGSVENRCRFPLEVVKAVVDEIGADKVGVRLSPYADYCECGDSNPHALGIYMAKSLSELGILYCHVIEPGMCTMFEKCDTNESLMPMRKIFNGTFIVAGGYNRTEGNNVLASNGADLVAYGRLFLANPDLPRRFELDAQLNKADKSTFYTNDPVVGYTDYPFLENATS*