AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047664.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047664.01.T01 MTR_4g132400 95.578 294 7 1 1 294 1 288 0.0 567
MsG0880047664.01.T01 MTR_4g049640 50.709 282 138 1 1 282 1 281 1.60e-83 258
MsG0880047664.01.T01 MTR_3g112530 52.941 238 110 2 45 281 43 279 9.51e-77 241
MsG0880047664.01.T01 MTR_4g101880 39.922 258 147 4 39 295 177 427 8.27e-54 186
MsG0880047664.01.T01 MTR_6g034030 37.269 271 161 5 25 295 215 476 4.87e-53 182
MsG0880047664.01.T01 MTR_6g034030 37.175 269 160 5 25 293 215 474 5.29e-52 182
MsG0880047664.01.T01 MTR_0549s0020 36.803 269 161 6 25 293 143 402 8.25e-51 177
MsG0880047664.01.T01 MTR_6g045200 36.431 269 162 6 25 293 206 465 9.58e-50 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047664.01.T01 AT1G62280 53.957 278 117 4 26 295 11 285 3.83e-90 276
MsG0880047664.01.T01 AT1G62262 52.128 282 113 4 19 295 13 277 1.44e-86 266
MsG0880047664.01.T01 AT5G24030 39.044 251 145 3 43 293 253 495 7.32e-53 182
MsG0880047664.01.T01 AT5G24030 39.044 251 145 3 43 293 253 495 8.71e-52 182
MsG0880047664.01.T01 AT4G27970 37.109 256 147 4 43 295 138 382 2.54e-48 167
MsG0880047664.01.T01 AT4G27970 37.109 256 147 4 43 295 138 382 2.54e-48 167
MsG0880047664.01.T01 AT1G12480 36.765 272 164 3 26 296 167 431 3.17e-48 171
MsG0880047664.01.T01 AT4G27970 37.109 256 147 4 43 295 138 382 2.75e-47 167
MsG0880047664.01.T01 AT4G27970 37.109 256 147 4 43 295 138 382 2.75e-47 167
MsG0880047664.01.T01 AT4G27970 37.705 244 138 4 55 295 1 233 1.40e-46 162
MsG0880047664.01.T01 AT4G27970 37.705 244 138 4 55 295 1 233 1.40e-46 162

Find 60 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATATATATAGGGTAATTT+TGG 0.163030 8:+89371400 None:intergenic
GTCAACCTGGTTTCTGTTAT+AGG 0.207016 8:-89371706 MsG0880047664.01.T01:CDS
CTTATATAATGCAGGCTTGC+AGG 0.280775 8:-89371335 MsG0880047664.01.T01:CDS
GCTAAGCCAAGGAATGTTAA+AGG 0.296789 8:+89371252 None:intergenic
TCAACCTGGTTTCTGTTATA+GGG 0.297612 8:-89371705 MsG0880047664.01.T01:CDS
GACCTACCTGAGAAATAAAA+AGG 0.303132 8:+89371422 None:intergenic
GAATGTGATGGCTTGGTGAT+TGG 0.310351 8:+89372195 None:intergenic
GCAATTTCAATGTATTCATT+AGG 0.313578 8:-89371634 MsG0880047664.01.T01:CDS
ATAAGCAACTAAATATATAT+AGG 0.322188 8:+89371390 None:intergenic
CTGTTGCTTATCTATTACTT+TGG 0.340533 8:-89372026 MsG0880047664.01.T01:CDS
GATGAATTTGCACATCATAT+TGG 0.345252 8:-89371925 MsG0880047664.01.T01:CDS
TCATTCCCTTTAACATTCCT+TGG 0.356219 8:-89371258 MsG0880047664.01.T01:CDS
CAAGCTAGACTTGCCATACT+TGG 0.362804 8:+89371505 None:intergenic
CACACAATTATGTCAATTGT+TGG 0.378332 8:+89372267 None:intergenic
ACAATGAATCACAAACTCTT+TGG 0.388818 8:-89372071 MsG0880047664.01.T01:CDS
CCTTGATCATTACAATGAAA+TGG 0.393096 8:+89371358 None:intergenic
CCATTTCATTGTAATGATCA+AGG 0.404012 8:-89371358 MsG0880047664.01.T01:CDS
AATGTGATGGCTTGGTGATT+GGG 0.406822 8:+89372196 None:intergenic
TAAGCAACTAAATATATATA+GGG 0.409041 8:+89371391 None:intergenic
TGCTTCAAGCATCACCGCCT+TGG 0.430111 8:-89371861 MsG0880047664.01.T01:CDS
GCTCAAGTTATGACAGAAAT+CGG 0.453671 8:-89371670 MsG0880047664.01.T01:CDS
ATAACAGAAACCAGGTTGAC+AGG 0.456215 8:+89371709 None:intergenic
GGTTTCTGTTATAGGGAACT+TGG 0.463993 8:-89371698 MsG0880047664.01.T01:CDS
TTGGTGCTCAAGCTTTGCTA+TGG 0.471950 8:-89372113 MsG0880047664.01.T01:CDS
TTAACTACATGTATACTCCT+TGG 0.476184 8:-89371900 MsG0880047664.01.T01:CDS
CTAAGCCAAGGAATGTTAAA+GGG 0.477768 8:+89371253 None:intergenic
TGACTCTGAATGTGATGGCT+TGG 0.485943 8:+89372188 None:intergenic
GAGCATATAAAGAAAGGATA+AGG 0.489737 8:+89371978 None:intergenic
GCATGGATCTTAGTTAAGAA+TGG 0.489812 8:+89372162 None:intergenic
TTCAATGTATTCATTAGGAA+TGG 0.492135 8:-89371629 MsG0880047664.01.T01:CDS
CAAGTATGGCAAGTCTAGCT+TGG 0.498344 8:-89371504 MsG0880047664.01.T01:CDS
CTAGCTTGGAAATCCATCTC+AGG 0.499285 8:-89371490 MsG0880047664.01.T01:CDS
GCACTTGAGCATATAAAGAA+AGG 0.500953 8:+89371972 None:intergenic
TGTTCAAGAAAACTATGAAA+AGG 0.514872 8:-89371305 MsG0880047664.01.T01:CDS
AGCACTAGTAGTATTGTTCG+TGG 0.515674 8:+89372239 None:intergenic
GTGATGGCTTGGTGATTGGG+TGG 0.520148 8:+89372199 None:intergenic
GATCAAGGCTTATATAATGC+AGG 0.526452 8:-89371343 MsG0880047664.01.T01:CDS
AGTAGCAAACCCTGTCAACC+TGG 0.530614 8:-89371719 MsG0880047664.01.T01:CDS
TTCTGTTATAGGGAACTTGG+TGG 0.536173 8:-89371695 MsG0880047664.01.T01:CDS
GAATCACAAACTCTTTGGCA+TGG 0.539825 8:-89372066 MsG0880047664.01.T01:CDS
AATGGTTCACTACTGAAAAG+AGG 0.541292 8:-89371753 MsG0880047664.01.T01:CDS
TAAGCTGGTCTTAGCACTAC+AGG 0.541582 8:+89371547 None:intergenic
ACTATAGAATAGAATGGGTG+AGG 0.549206 8:-89372301 None:intergenic
GACGAGAAAAGCACCTGAGA+TGG 0.550784 8:+89371477 None:intergenic
AAGTTATGACAGAAATCGGT+TGG 0.554540 8:-89371666 MsG0880047664.01.T01:CDS
TGATGGCTTGGTGATTGGGT+GGG 0.556380 8:+89372200 None:intergenic
CTTGCACTTTAATGCAGTGA+AGG 0.559661 8:-89371947 MsG0880047664.01.T01:CDS
TAGCAAAGCTTGAGCACCAA+AGG 0.566448 8:+89372116 None:intergenic
TTCTTAACTAAGATCCATGC+AGG 0.573358 8:-89372159 MsG0880047664.01.T01:CDS
TGAAAAGGCTAAACGTTACA+TGG 0.577666 8:-89371290 MsG0880047664.01.T01:CDS
TAACAGAAACCAGGTTGACA+GGG 0.582523 8:+89371710 None:intergenic
AGTAATAGATAAGCAACAGA+AGG 0.591520 8:+89372030 None:intergenic
ATGAGAAGATATGAAATCCA+AGG 0.593130 8:+89371883 None:intergenic
CATATAAAGAAAGGATAAGG+TGG 0.598970 8:+89371981 None:intergenic
GGTTGTGACTCTGAATGTGA+TGG 0.605496 8:+89372183 None:intergenic
ATTCAGCACAAGCTAAGCCA+AGG 0.637854 8:+89371241 None:intergenic
CAAGTTCTCGAAACAATCCA+AGG 0.648724 8:+89371844 None:intergenic
AAAGGCTAAACGTTACATGG+TGG 0.692493 8:-89371287 MsG0880047664.01.T01:CDS
AGTTCCCTATAACAGAAACC+AGG 0.725065 8:+89371701 None:intergenic
GTTCTCGAAACAATCCAAGG+CGG 0.791324 8:+89371847 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAGCAACTAAATATATAT+AGG + Chr8:89372144-89372163 None:intergenic 15.0%
!! TAAGCAACTAAATATATATA+GGG + Chr8:89372143-89372162 None:intergenic 15.0%
!!! AAATATATATAGGGTAATTT+TGG + Chr8:89372134-89372153 None:intergenic 15.0%
! GCAATTTCAATGTATTCATT+AGG - Chr8:89371897-89371916 MsG0880047664.01.T01:CDS 25.0%
! TGTTCAAGAAAACTATGAAA+AGG - Chr8:89372226-89372245 MsG0880047664.01.T01:CDS 25.0%
! TTCAATGTATTCATTAGGAA+TGG - Chr8:89371902-89371921 MsG0880047664.01.T01:CDS 25.0%
!! ATGTAAAACTTTTTGGACAA+TGG - Chr8:89371760-89371779 MsG0880047664.01.T01:CDS 25.0%
!! TTTTTCATTTGTGTTTCCTT+TGG - Chr8:89371399-89371418 MsG0880047664.01.T01:CDS 25.0%
!!! TTTGTTCCTTTTTATTTCTC+AGG - Chr8:89372103-89372122 MsG0880047664.01.T01:CDS 25.0%
ACAATGAATCACAAACTCTT+TGG - Chr8:89371460-89371479 MsG0880047664.01.T01:CDS 30.0%
AGTAATAGATAAGCAACAGA+AGG + Chr8:89371504-89371523 None:intergenic 30.0%
ATGAGAAGATATGAAATCCA+AGG + Chr8:89371651-89371670 None:intergenic 30.0%
CACACAATTATGTCAATTGT+TGG + Chr8:89371267-89371286 None:intergenic 30.0%
CATATAAAGAAAGGATAAGG+TGG + Chr8:89371553-89371572 None:intergenic 30.0%
CCATTTCATTGTAATGATCA+AGG - Chr8:89372173-89372192 MsG0880047664.01.T01:CDS 30.0%
CCTTGATCATTACAATGAAA+TGG + Chr8:89372176-89372195 None:intergenic 30.0%
GAGCATATAAAGAAAGGATA+AGG + Chr8:89371556-89371575 None:intergenic 30.0%
GATGAATTTGCACATCATAT+TGG - Chr8:89371606-89371625 MsG0880047664.01.T01:CDS 30.0%
GCAAAAAACAAGAAATAAGC+TGG + Chr8:89372002-89372021 None:intergenic 30.0%
TTAACTACATGTATACTCCT+TGG - Chr8:89371631-89371650 MsG0880047664.01.T01:CDS 30.0%
! CTGCTTGATGTAAAACTTTT+TGG - Chr8:89371753-89371772 MsG0880047664.01.T01:CDS 30.0%
! CTGTTGCTTATCTATTACTT+TGG - Chr8:89371505-89371524 MsG0880047664.01.T01:CDS 30.0%
!! TTCCTTTTTATTTCTCAGGT+AGG - Chr8:89372107-89372126 MsG0880047664.01.T01:CDS 30.0%
AAATGAAAAAGTAGCCTGCA+TGG + Chr8:89371389-89371408 None:intergenic 35.0%
AAGTTATGACAGAAATCGGT+TGG - Chr8:89371865-89371884 MsG0880047664.01.T01:CDS 35.0%
AATGGTTCACTACTGAAAAG+AGG - Chr8:89371778-89371797 MsG0880047664.01.T01:CDS 35.0%
CTAAGCCAAGGAATGTTAAA+GGG + Chr8:89372281-89372300 None:intergenic 35.0%
GACCTACCTGAGAAATAAAA+AGG + Chr8:89372112-89372131 None:intergenic 35.0%
GATCAAGGCTTATATAATGC+AGG - Chr8:89372188-89372207 MsG0880047664.01.T01:CDS 35.0%
GCACTTGAGCATATAAAGAA+AGG + Chr8:89371562-89371581 None:intergenic 35.0%
GCATGGATCTTAGTTAAGAA+TGG + Chr8:89371372-89371391 None:intergenic 35.0%
GCTCAAGTTATGACAGAAAT+CGG - Chr8:89371861-89371880 MsG0880047664.01.T01:CDS 35.0%
TCAACCTGGTTTCTGTTATA+GGG - Chr8:89371826-89371845 MsG0880047664.01.T01:CDS 35.0%
TCATTCCCTTTAACATTCCT+TGG - Chr8:89372273-89372292 MsG0880047664.01.T01:CDS 35.0%
TGAAAAGGCTAAACGTTACA+TGG - Chr8:89372241-89372260 MsG0880047664.01.T01:CDS 35.0%
TTCTTAACTAAGATCCATGC+AGG - Chr8:89371372-89371391 MsG0880047664.01.T01:CDS 35.0%
! ATAGTTTTCTTGAACAGTGC+TGG + Chr8:89372222-89372241 None:intergenic 35.0%
AAAGGCTAAACGTTACATGG+TGG - Chr8:89372244-89372263 MsG0880047664.01.T01:CDS 40.0%
AGCACTAGTAGTATTGTTCG+TGG + Chr8:89371295-89371314 None:intergenic 40.0%
AGTTCCCTATAACAGAAACC+AGG + Chr8:89371833-89371852 None:intergenic 40.0%
CAAGTTCTCGAAACAATCCA+AGG + Chr8:89371690-89371709 None:intergenic 40.0%
CTTATATAATGCAGGCTTGC+AGG - Chr8:89372196-89372215 MsG0880047664.01.T01:CDS 40.0%
CTTGCACTTTAATGCAGTGA+AGG - Chr8:89371584-89371603 MsG0880047664.01.T01:CDS 40.0%
GAATCACAAACTCTTTGGCA+TGG - Chr8:89371465-89371484 MsG0880047664.01.T01:CDS 40.0%
GCTAAGCCAAGGAATGTTAA+AGG + Chr8:89372282-89372301 None:intergenic 40.0%
GGTTTCTGTTATAGGGAACT+TGG - Chr8:89371833-89371852 MsG0880047664.01.T01:CDS 40.0%
GTCAACCTGGTTTCTGTTAT+AGG - Chr8:89371825-89371844 MsG0880047664.01.T01:CDS 40.0%
TTCTGTTATAGGGAACTTGG+TGG - Chr8:89371836-89371855 MsG0880047664.01.T01:CDS 40.0%
! ATAACAGAAACCAGGTTGAC+AGG + Chr8:89371825-89371844 None:intergenic 40.0%
! TAACAGAAACCAGGTTGACA+GGG + Chr8:89371824-89371843 None:intergenic 40.0%
!! AATGTGATGGCTTGGTGATT+GGG + Chr8:89371338-89371357 None:intergenic 40.0%
!!! GTTTTTTGCTGCACCAAGTA+TGG - Chr8:89372013-89372032 MsG0880047664.01.T01:CDS 40.0%
CAAGCTAGACTTGCCATACT+TGG + Chr8:89372029-89372048 None:intergenic 45.0%
CAAGTATGGCAAGTCTAGCT+TGG - Chr8:89372027-89372046 MsG0880047664.01.T01:CDS 45.0%
CTAGCTTGGAAATCCATCTC+AGG - Chr8:89372041-89372060 MsG0880047664.01.T01:CDS 45.0%
GGTTGTGACTCTGAATGTGA+TGG + Chr8:89371351-89371370 None:intergenic 45.0%
GTTCTCGAAACAATCCAAGG+CGG + Chr8:89371687-89371706 None:intergenic 45.0%
TAGCAAAGCTTGAGCACCAA+AGG + Chr8:89371418-89371437 None:intergenic 45.0%
TGACTCTGAATGTGATGGCT+TGG + Chr8:89371346-89371365 None:intergenic 45.0%
! TTGGTGCTCAAGCTTTGCTA+TGG - Chr8:89371418-89371437 MsG0880047664.01.T01:CDS 45.0%
!! GAATGTGATGGCTTGGTGAT+TGG + Chr8:89371339-89371358 None:intergenic 45.0%
!! TAAGCTGGTCTTAGCACTAC+AGG + Chr8:89371987-89372006 None:intergenic 45.0%
AGTAGCAAACCCTGTCAACC+TGG - Chr8:89371812-89371831 MsG0880047664.01.T01:CDS 50.0%
!! GACGAGAAAAGCACCTGAGA+TGG + Chr8:89372057-89372076 None:intergenic 50.0%
!! TGATGGCTTGGTGATTGGGT+GGG + Chr8:89371334-89371353 None:intergenic 50.0%
TGCTTCAAGCATCACCGCCT+TGG - Chr8:89371670-89371689 MsG0880047664.01.T01:CDS 55.0%
!! GTGATGGCTTGGTGATTGGG+TGG + Chr8:89371335-89371354 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 89371243 89372310 89371243 ID=MsG0880047664.01;Name=MsG0880047664.01
Chr8 mRNA 89371243 89372310 89371243 ID=MsG0880047664.01.T01;Parent=MsG0880047664.01;Name=MsG0880047664.01.T01;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|355
Chr8 exon 89371243 89372310 89371243 ID=MsG0880047664.01.T01:exon:18139;Parent=MsG0880047664.01.T01
Chr8 CDS 89371243 89372310 89371243 ID=MsG0880047664.01.T01:cds;Parent=MsG0880047664.01.T01
Gene Sequence

>MsG0880047664.01.T01

ATGGGTGAGGTTGCAATGAAGCCAACAATTGACATAATTGTGTGTGCTTCCACGAACAATACTACTAGTGCTAAAAACATTGATAATTCCCACCCAATCACCAAGCCATCACATTCAGAGTCACAACCATTCTTAACTAAGATCCATGCAGGCTACTTTTTCATTTGTGTTTCCTTTGGTGCTCAAGCTTTGCTATGGAAGAGTTTAAGTGAACATAACAATGAATCACAAACTCTTTGGCATGGATTTAATTTCATGCCTTCTGTTGCTTATCTATTACTTTGGTGTCTTGCAGTACTCATTGCAGCCACCTTATCCTTTCTTTATATGCTCAAGTGCATCTTGCACTTTAATGCAGTGAAGGATGAATTTGCACATCATATTGGAGTTAACTACATGTATACTCCTTGGATTTCATATCTTCTCATGCTTCAAGCATCACCGCCTTGGATTGTTTCGAGAACTTGTTACTACGAGTTTCTTTGTCTAGCATTTTCTTTTGTAATATTTCTGCTTGATGTAAAACTTTTTGGACAATGGTTCACTACTGAAAAGAGGTTTCTGTCAGTAGTAGCAAACCCTGTCAACCTGGTTTCTGTTATAGGGAACTTGGTGGCTGCTCAAGTTATGACAGAAATCGGTTGGAACGAGATTGCAATTTCAATGTATTCATTAGGAATGGTACATTATTTGATTCTATTTGTGACGCTGTATCAGCGTCTAACAAGTAGTAATCAGTTTCCTGTAGTGCTAAGACCAGCTTATTTCTTGTTTTTTGCTGCACCAAGTATGGCAAGTCTAGCTTGGAAATCCATCTCAGGTGCTTTTCTCGTCTCATCAAAGATGCTGTTCTTTCTCTCTTTGTTCCTTTTTATTTCTCAGGTAGGTCCAAAATTACCCTATATATATTTAGTTGCTTATTTTGATCATCCATTTCATTGTAATGATCAAGGCTTATATAATGCAGGCTTGCAGGCCAGCACTGTTCAAGAAAACTATGAAAAGGCTAAACGTTACATGGTGGATTTACTCATTCCCTTTAACATTCCTTGGCTTAGCTTGTGCTGA

Protein sequence

>MsG0880047664.01.T01

MGEVAMKPTIDIIVCASTNNTTSAKNIDNSHPITKPSHSESQPFLTKIHAGYFFICVSFGAQALLWKSLSEHNNESQTLWHGFNFMPSVAYLLLWCLAVLIAATLSFLYMLKCILHFNAVKDEFAHHIGVNYMYTPWISYLLMLQASPPWIVSRTCYYEFLCLAFSFVIFLLDVKLFGQWFTTEKRFLSVVANPVNLVSVIGNLVAAQVMTEIGWNEIAISMYSLGMVHYLILFVTLYQRLTSSNQFPVVLRPAYFLFFAAPSMASLAWKSISGAFLVSSKMLFFLSLFLFISQVGPKLPYIYLVAYFDHPFHCNDQGLYNAGLQASTVQENYEKAKRYMVDLLIPFNIPWLSLC*