Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019021.01.T01 | GAU28051.1 | 96.078 | 204 | 8 | 0 | 1 | 204 | 94 | 297 | 1.37E-138 | 406 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019021.01.T01 | Q9FKB6 | 71.014 | 207 | 41 | 2 | 1 | 204 | 126 | 316 | 4.70E-97 | 297 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019021.01.T01 | A0A2Z6M853 | 96.078 | 204 | 8 | 0 | 1 | 204 | 94 | 297 | 6.53e-139 | 406 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011282.01 | MsG0480019021.01 | 0.808441 | 3.092968e-50 | 1.768699e-47 |
| MsG0480019021.01 | MsG0780038386.01 | 0.839834 | 1.249926e-57 | 1.747291e-54 |
| MsG0480019021.01 | MsG0880042961.01 | 0.801984 | 6.954950e-49 | 3.367604e-46 |
| MsG0280009514.01 | MsG0480019021.01 | 0.801296 | 9.627530e-49 | 4.581402e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019021.01.T01 | MTR_4g022330 | 98.039 | 204 | 4 | 0 | 1 | 204 | 119 | 322 | 2.11e-142 | 411 |
| MsG0480019021.01.T01 | MTR_7g073980 | 41.553 | 219 | 99 | 5 | 1 | 204 | 119 | 323 | 2.87e-45 | 159 |
| MsG0480019021.01.T01 | MTR_5g016060 | 39.806 | 206 | 120 | 3 | 1 | 204 | 11 | 214 | 3.36e-41 | 146 |
| MsG0480019021.01.T01 | MTR_5g016060 | 39.806 | 206 | 120 | 3 | 1 | 204 | 125 | 328 | 1.20e-40 | 146 |
| MsG0480019021.01.T01 | MTR_7g108290 | 37.864 | 206 | 120 | 2 | 1 | 204 | 121 | 320 | 1.02e-37 | 138 |
| MsG0480019021.01.T01 | MTR_1g088885 | 34.951 | 206 | 122 | 3 | 1 | 204 | 121 | 316 | 1.71e-35 | 132 |
| MsG0480019021.01.T01 | MTR_3g062540 | 33.816 | 207 | 116 | 3 | 1 | 204 | 67 | 255 | 1.39e-34 | 129 |
| MsG0480019021.01.T01 | MTR_3g062540 | 33.816 | 207 | 116 | 3 | 1 | 204 | 109 | 297 | 2.04e-34 | 129 |
| MsG0480019021.01.T01 | MTR_4g095430 | 34.300 | 207 | 131 | 3 | 1 | 204 | 100 | 304 | 1.90e-32 | 123 |
| MsG0480019021.01.T01 | MTR_5g075640 | 32.093 | 215 | 117 | 4 | 1 | 204 | 67 | 263 | 2.01e-29 | 114 |
| MsG0480019021.01.T01 | MTR_5g075640 | 32.093 | 215 | 117 | 4 | 1 | 204 | 110 | 306 | 2.05e-29 | 115 |
| MsG0480019021.01.T01 | MTR_4g093850 | 31.837 | 245 | 126 | 4 | 1 | 204 | 129 | 373 | 2.53e-28 | 111 |
| MsG0480019021.01.T01 | MTR_2g009340 | 30.400 | 250 | 128 | 3 | 1 | 204 | 129 | 378 | 3.15e-28 | 112 |
| MsG0480019021.01.T01 | MTR_4g093850 | 31.837 | 245 | 126 | 4 | 1 | 204 | 129 | 373 | 4.30e-28 | 111 |
| MsG0480019021.01.T01 | MTR_2g048390 | 31.081 | 222 | 135 | 3 | 1 | 204 | 112 | 333 | 9.53e-28 | 109 |
| MsG0480019021.01.T01 | MTR_8g078900 | 31.674 | 221 | 130 | 4 | 1 | 204 | 85 | 301 | 8.83e-27 | 106 |
| MsG0480019021.01.T01 | MTR_8g078900 | 31.674 | 221 | 130 | 4 | 1 | 204 | 125 | 341 | 1.11e-26 | 106 |
| MsG0480019021.01.T01 | MTR_2g084595 | 27.354 | 223 | 142 | 3 | 1 | 204 | 110 | 331 | 1.12e-26 | 106 |
| MsG0480019021.01.T01 | MTR_8g078900 | 31.674 | 221 | 130 | 4 | 1 | 204 | 95 | 311 | 2.26e-26 | 106 |
| MsG0480019021.01.T01 | MTR_8g078900 | 31.674 | 221 | 130 | 4 | 1 | 204 | 125 | 341 | 2.31e-26 | 106 |
| MsG0480019021.01.T01 | MTR_8g078900 | 31.674 | 221 | 130 | 4 | 1 | 204 | 85 | 301 | 2.36e-26 | 106 |
| MsG0480019021.01.T01 | MTR_2g084595 | 27.354 | 223 | 142 | 3 | 1 | 204 | 110 | 331 | 3.89e-26 | 105 |
| MsG0480019021.01.T01 | MTR_1g064360 | 30.000 | 210 | 126 | 6 | 1 | 204 | 113 | 307 | 1.08e-24 | 101 |
| MsG0480019021.01.T01 | MTR_8g090220 | 29.545 | 220 | 124 | 4 | 1 | 204 | 138 | 342 | 3.41e-24 | 100 |
| MsG0480019021.01.T01 | MTR_8g090220 | 29.545 | 220 | 124 | 4 | 1 | 204 | 138 | 342 | 3.51e-24 | 100 |
| MsG0480019021.01.T01 | MTR_5g015790 | 30.093 | 216 | 134 | 4 | 1 | 206 | 111 | 319 | 3.51e-24 | 100 |
| MsG0480019021.01.T01 | MTR_1g094850 | 31.776 | 214 | 132 | 6 | 1 | 204 | 112 | 321 | 6.34e-24 | 99.4 |
| MsG0480019021.01.T01 | MTR_2g018750 | 28.704 | 216 | 141 | 5 | 1 | 204 | 113 | 327 | 2.29e-23 | 97.8 |
| MsG0480019021.01.T01 | MTR_1g082690 | 31.373 | 204 | 120 | 4 | 1 | 204 | 147 | 330 | 1.66e-22 | 95.1 |
| MsG0480019021.01.T01 | MTR_5g032780 | 31.222 | 221 | 134 | 5 | 1 | 204 | 113 | 332 | 2.91e-21 | 91.7 |
| MsG0480019021.01.T01 | MTR_4g134210 | 29.469 | 207 | 138 | 4 | 1 | 203 | 111 | 313 | 1.26e-20 | 89.7 |
| MsG0480019021.01.T01 | MTR_8g069330 | 32.850 | 207 | 123 | 5 | 1 | 204 | 112 | 305 | 9.68e-20 | 87.4 |
| MsG0480019021.01.T01 | MTR_1g014070 | 31.628 | 215 | 121 | 7 | 1 | 200 | 112 | 315 | 2.19e-19 | 86.3 |
| MsG0480019021.01.T01 | MTR_1g106430 | 27.907 | 215 | 139 | 4 | 1 | 206 | 113 | 320 | 4.76e-19 | 85.5 |
| MsG0480019021.01.T01 | MTR_2g027410 | 26.699 | 206 | 144 | 3 | 1 | 203 | 39 | 240 | 1.24e-14 | 72.4 |
| MsG0480019021.01.T01 | MTR_2g027410 | 26.699 | 206 | 144 | 3 | 1 | 203 | 111 | 312 | 1.45e-14 | 72.4 |
| MsG0480019021.01.T01 | MTR_4g011530 | 35.000 | 120 | 70 | 3 | 93 | 208 | 46 | 161 | 3.21e-14 | 71.2 |
| MsG0480019021.01.T01 | MTR_2g102730 | 34.167 | 120 | 73 | 2 | 93 | 208 | 24 | 141 | 2.43e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019021.01.T01 | AT5G48800 | 71.014 | 207 | 41 | 2 | 1 | 204 | 126 | 316 | 4.79e-98 | 297 |
| MsG0480019021.01.T01 | AT3G08570 | 43.750 | 208 | 104 | 6 | 1 | 204 | 121 | 319 | 1.77e-43 | 154 |
| MsG0480019021.01.T01 | AT5G67385 | 40.097 | 207 | 116 | 4 | 1 | 204 | 66 | 267 | 2.77e-38 | 139 |
| MsG0480019021.01.T01 | AT5G67385 | 40.097 | 207 | 116 | 4 | 1 | 204 | 66 | 267 | 2.77e-38 | 139 |
| MsG0480019021.01.T01 | AT5G67385 | 40.097 | 207 | 116 | 4 | 1 | 204 | 66 | 267 | 2.77e-38 | 139 |
| MsG0480019021.01.T01 | AT5G67385 | 40.097 | 207 | 116 | 4 | 1 | 204 | 66 | 267 | 2.77e-38 | 139 |
| MsG0480019021.01.T01 | AT5G67385 | 40.097 | 207 | 116 | 4 | 1 | 204 | 111 | 312 | 3.53e-38 | 139 |
| MsG0480019021.01.T01 | AT2G47860 | 36.538 | 208 | 123 | 4 | 1 | 206 | 119 | 319 | 9.28e-38 | 136 |
| MsG0480019021.01.T01 | AT2G47860 | 36.538 | 208 | 123 | 4 | 1 | 206 | 142 | 342 | 1.43e-37 | 136 |
| MsG0480019021.01.T01 | AT2G47860 | 36.538 | 208 | 123 | 4 | 1 | 206 | 1 | 201 | 4.21e-37 | 135 |
| MsG0480019021.01.T01 | AT2G47860 | 36.538 | 208 | 123 | 4 | 1 | 206 | 1 | 201 | 4.21e-37 | 135 |
| MsG0480019021.01.T01 | AT2G47860 | 36.538 | 208 | 123 | 4 | 1 | 206 | 119 | 319 | 6.70e-37 | 136 |
| MsG0480019021.01.T01 | AT2G47860 | 36.538 | 208 | 123 | 4 | 1 | 206 | 142 | 342 | 7.89e-37 | 136 |
| MsG0480019021.01.T01 | AT3G49970 | 37.561 | 205 | 105 | 3 | 1 | 204 | 78 | 260 | 1.08e-35 | 132 |
| MsG0480019021.01.T01 | AT3G49970 | 37.561 | 205 | 105 | 3 | 1 | 204 | 78 | 260 | 1.08e-35 | 132 |
| MsG0480019021.01.T01 | AT2G30520 | 30.806 | 211 | 119 | 3 | 1 | 204 | 67 | 257 | 1.01e-33 | 126 |
| MsG0480019021.01.T01 | AT2G30520 | 30.806 | 211 | 119 | 3 | 1 | 204 | 67 | 257 | 1.01e-33 | 126 |
| MsG0480019021.01.T01 | AT2G30520 | 30.806 | 211 | 119 | 3 | 1 | 204 | 115 | 305 | 1.21e-33 | 127 |
| MsG0480019021.01.T01 | AT1G03010 | 34.742 | 213 | 124 | 5 | 1 | 204 | 118 | 324 | 6.61e-32 | 122 |
| MsG0480019021.01.T01 | AT1G03010 | 34.742 | 213 | 124 | 5 | 1 | 204 | 91 | 297 | 7.18e-32 | 122 |
| MsG0480019021.01.T01 | AT1G03010 | 34.762 | 210 | 128 | 4 | 1 | 204 | 117 | 323 | 7.86e-32 | 122 |
| MsG0480019021.01.T01 | AT3G08660 | 37.736 | 212 | 102 | 6 | 1 | 206 | 118 | 305 | 8.93e-31 | 117 |
| MsG0480019021.01.T01 | AT3G08660 | 37.736 | 212 | 102 | 6 | 1 | 206 | 58 | 245 | 1.85e-30 | 117 |
| MsG0480019021.01.T01 | AT3G08660 | 37.736 | 212 | 102 | 6 | 1 | 206 | 118 | 305 | 2.65e-30 | 117 |
| MsG0480019021.01.T01 | AT5G03250 | 32.018 | 228 | 126 | 6 | 1 | 206 | 1 | 221 | 1.53e-27 | 109 |
| MsG0480019021.01.T01 | AT5G03250 | 32.018 | 228 | 126 | 6 | 1 | 206 | 113 | 333 | 2.32e-27 | 109 |
| MsG0480019021.01.T01 | AT5G13600 | 33.182 | 220 | 131 | 8 | 1 | 204 | 110 | 329 | 3.84e-24 | 100 |
| MsG0480019021.01.T01 | AT3G44820 | 27.826 | 230 | 140 | 3 | 1 | 204 | 111 | 340 | 9.32e-24 | 99.0 |
| MsG0480019021.01.T01 | AT1G30440 | 27.803 | 223 | 142 | 4 | 1 | 204 | 113 | 335 | 1.02e-21 | 93.2 |
| MsG0480019021.01.T01 | AT5G67440 | 29.808 | 208 | 135 | 4 | 1 | 204 | 78 | 278 | 1.43e-21 | 92.4 |
| MsG0480019021.01.T01 | AT5G67440 | 29.808 | 208 | 135 | 4 | 1 | 204 | 115 | 315 | 1.55e-21 | 92.4 |
| MsG0480019021.01.T01 | AT5G67440 | 29.808 | 208 | 135 | 4 | 1 | 204 | 115 | 315 | 1.55e-21 | 92.4 |
| MsG0480019021.01.T01 | AT5G66560 | 30.837 | 227 | 129 | 6 | 1 | 204 | 148 | 369 | 1.63e-21 | 92.4 |
| MsG0480019021.01.T01 | AT5G66560 | 30.837 | 227 | 129 | 6 | 1 | 204 | 119 | 340 | 2.91e-21 | 91.7 |
| MsG0480019021.01.T01 | AT5G66560 | 30.837 | 227 | 129 | 6 | 1 | 204 | 148 | 369 | 3.16e-21 | 91.7 |
| MsG0480019021.01.T01 | AT5G66560 | 30.837 | 227 | 129 | 6 | 1 | 204 | 109 | 330 | 3.31e-21 | 91.7 |
| MsG0480019021.01.T01 | AT2G14820 | 28.774 | 212 | 138 | 4 | 1 | 206 | 112 | 316 | 4.38e-21 | 91.3 |
| MsG0480019021.01.T01 | AT2G14820 | 28.774 | 212 | 138 | 4 | 1 | 206 | 112 | 316 | 4.38e-21 | 91.3 |
| MsG0480019021.01.T01 | AT3G50840 | 30.476 | 210 | 137 | 4 | 1 | 204 | 102 | 308 | 1.82e-20 | 89.4 |
| MsG0480019021.01.T01 | AT3G50840 | 30.476 | 210 | 137 | 4 | 1 | 204 | 66 | 272 | 2.54e-20 | 89.0 |
| MsG0480019021.01.T01 | AT3G50840 | 30.476 | 210 | 137 | 4 | 1 | 204 | 66 | 272 | 2.54e-20 | 89.0 |
| MsG0480019021.01.T01 | AT5G64330 | 34.395 | 157 | 80 | 2 | 1 | 134 | 137 | 293 | 1.18e-19 | 87.0 |
| MsG0480019021.01.T01 | AT5G64330 | 34.395 | 157 | 80 | 2 | 1 | 134 | 137 | 293 | 1.30e-19 | 87.0 |
| MsG0480019021.01.T01 | AT5G64330 | 34.395 | 157 | 80 | 2 | 1 | 134 | 78 | 234 | 1.55e-19 | 86.7 |
| MsG0480019021.01.T01 | AT4G37590 | 30.769 | 195 | 125 | 5 | 1 | 192 | 123 | 310 | 3.61e-19 | 85.9 |
| MsG0480019021.01.T01 | AT2G23050 | 29.327 | 208 | 129 | 7 | 1 | 204 | 1 | 194 | 2.36e-18 | 82.8 |
| MsG0480019021.01.T01 | AT5G10250 | 27.477 | 222 | 127 | 5 | 1 | 204 | 136 | 341 | 3.33e-18 | 82.8 |
| MsG0480019021.01.T01 | AT5G10250 | 27.477 | 222 | 127 | 5 | 1 | 204 | 136 | 341 | 3.60e-18 | 82.8 |
| MsG0480019021.01.T01 | AT2G23050 | 29.327 | 208 | 129 | 7 | 1 | 204 | 117 | 310 | 4.69e-18 | 82.4 |
| MsG0480019021.01.T01 | AT4G31820 | 28.638 | 213 | 129 | 6 | 1 | 200 | 112 | 314 | 2.24e-17 | 80.5 |
| MsG0480019021.01.T01 | AT4G31820 | 28.638 | 213 | 129 | 6 | 1 | 200 | 59 | 261 | 2.40e-17 | 80.5 |
| MsG0480019021.01.T01 | AT3G26490 | 31.429 | 210 | 110 | 7 | 8 | 200 | 124 | 316 | 6.00e-17 | 79.3 |
| MsG0480019021.01.T01 | AT3G26490 | 31.429 | 210 | 110 | 7 | 8 | 200 | 124 | 316 | 9.89e-17 | 78.6 |
| MsG0480019021.01.T01 | AT3G26490 | 31.429 | 210 | 110 | 7 | 8 | 200 | 124 | 316 | 9.89e-17 | 78.6 |
| MsG0480019021.01.T01 | AT3G26490 | 31.429 | 210 | 110 | 7 | 8 | 200 | 124 | 316 | 9.89e-17 | 78.6 |
| MsG0480019021.01.T01 | AT1G67900 | 31.628 | 215 | 133 | 4 | 1 | 204 | 108 | 319 | 9.93e-17 | 78.6 |
| MsG0480019021.01.T01 | AT1G67900 | 31.628 | 215 | 133 | 4 | 1 | 204 | 108 | 319 | 9.93e-17 | 78.6 |
| MsG0480019021.01.T01 | AT1G67900 | 31.628 | 215 | 133 | 4 | 1 | 204 | 108 | 319 | 9.93e-17 | 78.6 |
| MsG0480019021.01.T01 | AT1G67900 | 31.628 | 215 | 133 | 4 | 1 | 204 | 108 | 319 | 9.93e-17 | 78.6 |
| MsG0480019021.01.T01 | AT1G67900 | 31.628 | 215 | 133 | 4 | 1 | 204 | 108 | 319 | 9.93e-17 | 78.6 |
| MsG0480019021.01.T01 | AT1G67900 | 31.628 | 215 | 133 | 4 | 1 | 204 | 108 | 319 | 9.93e-17 | 78.6 |
| MsG0480019021.01.T01 | AT5G47800 | 26.699 | 206 | 144 | 3 | 1 | 203 | 224 | 425 | 3.89e-14 | 71.2 |
| MsG0480019021.01.T01 | AT5G47800 | 26.699 | 206 | 144 | 3 | 1 | 203 | 111 | 312 | 4.71e-14 | 70.9 |
| MsG0480019021.01.T01 | AT5G47800 | 26.699 | 206 | 144 | 3 | 1 | 203 | 111 | 312 | 4.71e-14 | 70.9 |
| MsG0480019021.01.T01 | AT5G47800 | 26.699 | 206 | 144 | 3 | 1 | 203 | 111 | 312 | 4.71e-14 | 70.9 |
| MsG0480019021.01.T01 | AT3G15570 | 32.231 | 121 | 66 | 3 | 102 | 208 | 38 | 156 | 1.49e-12 | 66.2 |
| MsG0480019021.01.T01 | AT5G48130 | 23.176 | 233 | 137 | 6 | 1 | 213 | 120 | 330 | 2.87e-12 | 65.5 |
| MsG0480019021.01.T01 | AT1G52770 | 27.500 | 160 | 95 | 3 | 59 | 208 | 3 | 151 | 3.40e-11 | 62.4 |
Find 32 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCATCGGCTAGAGGAATT+AGG | 0.287518 | 4:+13393709 | None:intergenic |
| AGGGAAGCACCGATACTTTC+TGG | 0.336799 | 4:+13393468 | None:intergenic |
| TTGCATTGTCTGCAAGAATC+TGG | 0.342422 | 4:-13393766 | MsG0480019021.01.T01:CDS |
| TGACAAAGAAATCAAGTTTG+TGG | 0.407412 | 4:-13393422 | MsG0480019021.01.T01:CDS |
| CGATGAAGTGTCGTGGAGTT+CGG | 0.433880 | 4:-13393491 | MsG0480019021.01.T01:CDS |
| AGATTAGAGTATAGCAGCTC+AGG | 0.434607 | 4:-13393612 | MsG0480019021.01.T01:CDS |
| AGCCCGAAAAGGAAATTAAT+CGG | 0.442233 | 4:+13393297 | None:intergenic |
| TTGGTGGATAGAAGATATTT+CGG | 0.448462 | 4:-13393550 | MsG0480019021.01.T01:CDS |
| CGCTTCGCAGCAGCCCGAAA+AGG | 0.462036 | 4:+13393286 | None:intergenic |
| GAAACTAGAAGCTATTTGTT+CGG | 0.465540 | 4:+13393638 | None:intergenic |
| CAAGTTTCTCAACAGGCAAA+AGG | 0.468440 | 4:+13393325 | None:intergenic |
| GGAACAACAAGTTTCTCAAC+AGG | 0.490954 | 4:+13393318 | None:intergenic |
| CTTTCTCTGCGTAGTTCACA+AGG | 0.493853 | 4:+13393448 | None:intergenic |
| AATTTCTGCTAGGCTTGACT+TGG | 0.501655 | 4:-13393244 | MsG0480019021.01.T01:CDS |
| TTGATTGCGCAATTTCTGCT+AGG | 0.508897 | 4:-13393254 | MsG0480019021.01.T01:CDS |
| CAAAAGATAATCTCAGTTCG+AGG | 0.515617 | 4:-13393806 | MsG0480019021.01.T01:CDS |
| CTAGGCTTGACTTGGAAAGA+AGG | 0.527201 | 4:-13393236 | MsG0480019021.01.T01:CDS |
| TTTCGGGCTGCTGCGAAGCG+CGG | 0.527819 | 4:-13393283 | MsG0480019021.01.T01:CDS |
| TAATCTCAGTTCGAGGGCCG+AGG | 0.540316 | 4:-13393799 | MsG0480019021.01.T01:CDS |
| AAAGAAGGATGGATCACAGT+TGG | 0.559409 | 4:-13393221 | MsG0480019021.01.T01:CDS |
| GCTTGACTTGGAAAGAAGGA+TGG | 0.567918 | 4:-13393232 | MsG0480019021.01.T01:CDS |
| CTAAATGTGATGGCGATTGG+TGG | 0.570724 | 4:-13393566 | MsG0480019021.01.T01:CDS |
| AATGCAATCAAGATATTCCT+CGG | 0.577379 | 4:+13393782 | None:intergenic |
| AAGCTAAATGTGATGGCGAT+TGG | 0.581729 | 4:-13393569 | MsG0480019021.01.T01:CDS |
| AGCAGACAAGCTAAATGTGA+TGG | 0.595602 | 4:-13393576 | MsG0480019021.01.T01:CDS |
| GGAGTTCGGCCAGAAAGTAT+CGG | 0.596918 | 4:-13393477 | MsG0480019021.01.T01:CDS |
| AACTTTGCATGAAAAGCTTG+TGG | 0.598754 | 4:-13393364 | MsG0480019021.01.T01:CDS |
| AATCAAAATATCATCGAGTG+TGG | 0.604727 | 4:+13393192 | None:intergenic |
| AAAAGATAATCTCAGTTCGA+GGG | 0.616789 | 4:-13393805 | MsG0480019021.01.T01:CDS |
| TAGAGTATAGCAGCTCAGGT+AGG | 0.658441 | 4:-13393608 | MsG0480019021.01.T01:CDS |
| ATAACAGCGATGAAGTGTCG+TGG | 0.667269 | 4:-13393498 | MsG0480019021.01.T01:CDS |
| TTTCTCTGCGTAGTTCACAA+GGG | 0.694419 | 4:+13393449 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTGAATCAGTTTTATTC+TGG | + | Chr4:13393631-13393650 | None:intergenic | 20.0% |
| !!! | ATTATCTTTTGAGAAATCAT+CGG | + | Chr4:13393201-13393220 | None:intergenic | 20.0% |
| AAAAGATAATCTCAGTTCGA+GGG | - | Chr4:13393211-13393230 | MsG0480019021.01.T01:CDS | 30.0% | |
| AATGCAATCAAGATATTCCT+CGG | + | Chr4:13393237-13393256 | None:intergenic | 30.0% | |
| GAAACTAGAAGCTATTTGTT+CGG | + | Chr4:13393381-13393400 | None:intergenic | 30.0% | |
| TGACAAAGAAATCAAGTTTG+TGG | - | Chr4:13393594-13393613 | MsG0480019021.01.T01:CDS | 30.0% | |
| ! | AGTAACTATTTTCAGCTCAT+CGG | + | Chr4:13393324-13393343 | None:intergenic | 30.0% |
| ! | GTTCCGATTAATTTCCTTTT+CGG | - | Chr4:13393716-13393735 | MsG0480019021.01.T01:CDS | 30.0% |
| ! | TTCCGATTAATTTCCTTTTC+GGG | - | Chr4:13393717-13393736 | MsG0480019021.01.T01:CDS | 30.0% |
| ! | TTGGTGGATAGAAGATATTT+CGG | - | Chr4:13393466-13393485 | MsG0480019021.01.T01:CDS | 30.0% |
| AACTTTGCATGAAAAGCTTG+TGG | - | Chr4:13393652-13393671 | MsG0480019021.01.T01:CDS | 35.0% | |
| AGCCCGAAAAGGAAATTAAT+CGG | + | Chr4:13393722-13393741 | None:intergenic | 35.0% | |
| CAAAAGATAATCTCAGTTCG+AGG | - | Chr4:13393210-13393229 | MsG0480019021.01.T01:CDS | 35.0% | |
| AAAGAAGGATGGATCACAGT+TGG | - | Chr4:13393795-13393814 | MsG0480019021.01.T01:CDS | 40.0% | |
| AATTTCTGCTAGGCTTGACT+TGG | - | Chr4:13393772-13393791 | MsG0480019021.01.T01:CDS | 40.0% | |
| AGATTAGAGTATAGCAGCTC+AGG | - | Chr4:13393404-13393423 | MsG0480019021.01.T01:CDS | 40.0% | |
| AGCAGACAAGCTAAATGTGA+TGG | - | Chr4:13393440-13393459 | MsG0480019021.01.T01:CDS | 40.0% | |
| CAAGTTTCTCAACAGGCAAA+AGG | + | Chr4:13393694-13393713 | None:intergenic | 40.0% | |
| GGAACAACAAGTTTCTCAAC+AGG | + | Chr4:13393701-13393720 | None:intergenic | 40.0% | |
| TTGATTGCGCAATTTCTGCT+AGG | - | Chr4:13393762-13393781 | MsG0480019021.01.T01:CDS | 40.0% | |
| TTGCATTGTCTGCAAGAATC+TGG | - | Chr4:13393250-13393269 | MsG0480019021.01.T01:CDS | 40.0% | |
| TTTCTCTGCGTAGTTCACAA+GGG | + | Chr4:13393570-13393589 | None:intergenic | 40.0% | |
| ! | AAGCTAAATGTGATGGCGAT+TGG | - | Chr4:13393447-13393466 | MsG0480019021.01.T01:CDS | 40.0% |
| CTTTCTCTGCGTAGTTCACA+AGG | + | Chr4:13393571-13393590 | None:intergenic | 45.0% | |
| TAGAGTATAGCAGCTCAGGT+AGG | - | Chr4:13393408-13393427 | MsG0480019021.01.T01:CDS | 45.0% | |
| ! | CTAAATGTGATGGCGATTGG+TGG | - | Chr4:13393450-13393469 | MsG0480019021.01.T01:CDS | 45.0% |
| !! | ATAACAGCGATGAAGTGTCG+TGG | - | Chr4:13393518-13393537 | MsG0480019021.01.T01:CDS | 45.0% |
| !! | ATTTTCAGCTCATCGGCTAG+AGG | + | Chr4:13393317-13393336 | None:intergenic | 45.0% |
| !! | CTAGGCTTGACTTGGAAAGA+AGG | - | Chr4:13393780-13393799 | MsG0480019021.01.T01:CDS | 45.0% |
| !! | GCTTGACTTGGAAAGAAGGA+TGG | - | Chr4:13393784-13393803 | MsG0480019021.01.T01:CDS | 45.0% |
| ! | GCTCATCGGCTAGAGGAATT+AGG | + | Chr4:13393310-13393329 | None:intergenic | 50.0% |
| ! | GGAGTTCGGCCAGAAAGTAT+CGG | - | Chr4:13393539-13393558 | MsG0480019021.01.T01:CDS | 50.0% |
| !! | AGGGAAGCACCGATACTTTC+TGG | + | Chr4:13393551-13393570 | None:intergenic | 50.0% |
| !! | CGATGAAGTGTCGTGGAGTT+CGG | - | Chr4:13393525-13393544 | MsG0480019021.01.T01:CDS | 50.0% |
| TAATCTCAGTTCGAGGGCCG+AGG | - | Chr4:13393217-13393236 | MsG0480019021.01.T01:CDS | 55.0% | |
| CGCTTCGCAGCAGCCCGAAA+AGG | + | Chr4:13393733-13393752 | None:intergenic | 65.0% | |
| TTTCGGGCTGCTGCGAAGCG+CGG | - | Chr4:13393733-13393752 | MsG0480019021.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 13393194 | 13393844 | 13393194 | ID=MsG0480019021.01;Name=MsG0480019021.01 |
| Chr4 | mRNA | 13393194 | 13393844 | 13393194 | ID=MsG0480019021.01.T01;Parent=MsG0480019021.01;Name=MsG0480019021.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|216 |
| Chr4 | exon | 13393194 | 13393844 | 13393194 | ID=MsG0480019021.01.T01:exon:8389;Parent=MsG0480019021.01.T01 |
| Chr4 | CDS | 13393194 | 13393844 | 13393194 | ID=MsG0480019021.01.T01:cds;Parent=MsG0480019021.01.T01 |
| Gene Sequence |
| Protein sequence |
