AlfalfaGEDB | MsG0480019274.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019274.01

KEGG: -
GO: GO:0000079 GO:0019901
PFAM: PF08613

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019274.01.T01	XP_003605284.1	99.541	218	1	0	1	218	1	218	2.19E-158	447
MsG0480019274.01.T02	XP_003605284.1	99.432	176	1	0	1	176	43	218	8.10E-126	362
MsG0480019274.01.T03	XP_003605284.1	98.913	92	1	0	1	92	127	218	5.71E-59	189
MsG0480019274.01.T04	XP_014490349.1	82.609	92	11	1	2	88	134	225	8.16E-45	154

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019274.01.T01	Q9SHD3	72.768	224	47	3	5	218	3	222	1.06E-113	327
MsG0480019274.01.T02	Q9SHD3	71.892	185	39	2	1	176	42	222	1.31E-92	271
MsG0480019274.01.T03	Q9SHD3	72.277	101	19	1	1	92	122	222	5.49E-46	149
MsG0480019274.01.T04	Q9SHD3	66.667	96	23	1	2	88	127	222	3.19E-38	129

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019274.01.T01	G7JIA5	99.541	218	1	0	1	218	1	218	1.04e-158	447
MsG0480019274.01.T02	G7JIA5	99.432	176	1	0	1	176	43	218	3.87e-126	362
MsG0480019274.01.T03	G7JIA5	98.913	92	1	0	1	92	127	218	2.73e-59	189
MsG0480019274.01.T04	A0A0S3SXN7	82.609	92	11	1	2	88	134	225	3.90e-45	154

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005272.01	MsG0480019274.01	0.803338	3.655997e-49	1.832454e-46
MsG0380017997.01	MsG0480019274.01	0.824468	7.939610e-54	7.038408e-51
MsG0480019274.01	MsG0580025746.01	0.850348	1.799891e-60	3.515126e-57

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PPI

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Gene1	Gene2	Type
MsG0480019274.01	MsG0780040639.01	PPI
MsG0480019274.01	MsG0780040639.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019274.01.T01	MTR_4g028060	99.541	218	1	0	1	218	1	218	2.65e-162	447
MsG0480019274.01.T01	MTR_7g081130	46.541	159	79	2	29	187	11	163	1.06e-45	150
MsG0480019274.01.T01	MTR_8g007380	45.732	164	81	1	24	187	5	160	1.35e-45	150
MsG0480019274.01.T01	MTR_7g094880	47.541	122	63	1	65	186	63	183	3.94e-37	129
MsG0480019274.01.T01	MTR_7g094880	49.565	115	57	1	72	186	85	198	5.43e-37	129
MsG0480019274.01.T01	MTR_2g088860	43.169	183	93	3	27	199	24	205	1.84e-33	120
MsG0480019274.01.T02	MTR_4g028060	99.432	176	1	0	1	176	43	218	9.81e-130	362
MsG0480019274.01.T02	MTR_7g081130	50.769	130	64	0	16	145	34	163	1.16e-44	146
MsG0480019274.01.T02	MTR_8g007380	52.846	123	58	0	23	145	38	160	3.17e-43	142
MsG0480019274.01.T02	MTR_7g094880	47.541	122	63	1	23	144	63	183	1.56e-37	129

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019274.01.T01	AT2G45080	72.768	224	47	3	5	218	3	222	1.08e-114	327
MsG0480019274.01.T01	AT3G60550	70.044	227	57	1	3	218	4	230	4.25e-113	323
MsG0480019274.01.T01	AT2G44740	44.654	159	84	2	31	189	13	167	1.08e-43	145
MsG0480019274.01.T01	AT5G07450	40.351	171	86	2	29	193	21	181	6.86e-41	139
MsG0480019274.01.T01	AT5G61650	40.373	161	88	1	29	189	23	175	6.25e-39	134
MsG0480019274.01.T01	AT3G63120	42.958	142	78	2	59	200	58	196	2.79e-38	132
MsG0480019274.01.T01	AT3G63120	42.958	142	78	2	59	200	58	196	2.79e-38	132
MsG0480019274.01.T01	AT3G21870	39.394	165	97	2	25	189	21	182	1.15e-35	125
MsG0480019274.01.T01	AT3G05327	37.725	167	97	2	25	187	27	190	5.91e-33	118
MsG0480019274.01.T02	AT2G45080	71.892	185	39	2	1	176	42	222	1.33e-93	271

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Find 29 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAATGAATTGAATGAGT+TGG	0.500361	4:-17563565	MsG0480019274.01.T01:CDS
AATTCATACTTTGCAAAAGT+TGG	0.389350	4:-17563597	MsG0480019274.01.T01:CDS
ACCACATTGAGAGGACCTTA+AGG	0.502225	4:-17563437	MsG0480019274.01.T01:CDS
ACCTTAAGGTCCTCTCAATG+TGG	0.678220	4:+17563436	None:intergenic
ACTTCTCATAACAACTATTA+TGG	0.285093	4:-17564273	MsG0480019274.01.T01:CDS
AGGGAAGTAAGCATTGGAGG+AGG	0.444484	4:-17563462	MsG0480019274.01.T01:CDS
ATACACACGTGCTGGACCTT+CGG	0.437594	4:-17564378	MsG0480019274.01.T01:CDS
CTAGAACATTTATCACTAAT+GGG	0.347684	4:+17564549	None:intergenic
CTATTGTTCCCATTTGGAGA+GGG	0.594811	4:-17563481	MsG0480019274.01.T01:CDS
GAGAGGGAAGTAAGCATTGG+AGG	0.422508	4:-17563465	MsG0480019274.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTATATTGCCTCAAAGCA+TGG	-	Chr4:17563943-17563962	MsG0480019274.01.T01:intron	30.0%
ACGGACATTAATTGCATTAA+TGG	-	Chr4:17564088-17564107	MsG0480019274.01.T01:intron	30.0%
AGTGAAGACCAGAAAAATAT+TGG	+	Chr4:17563993-17564012	None:intergenic	30.0%
CAAGGTAACAATTTGGTAAA+GGG	-	Chr4:17563875-17563894	MsG0480019274.01.T01:intron	30.0%
GCTAGAACATTTATCACTAA+TGG	+	Chr4:17563467-17563486	None:intergenic	30.0%
GTGAAGACCAGAAAAATATT+GGG	+	Chr4:17563992-17564011	None:intergenic	30.0%
TCAAGGTAACAATTTGGTAA+AGG	-	Chr4:17563874-17563893	MsG0480019274.01.T01:intron	30.0%
TGAAGACCAGAAAAATATTG+GGG	+	Chr4:17563991-17564010	None:intergenic	30.0%
CTCTTTAATCTGACGGTTTA+CGG	-	Chr4:17564069-17564088	MsG0480019274.01.T01:intron	35.0%
GCTGAAGAAATCAAAGAAAG+AGG	-	Chr4:17564601-17564620	MsG0480019274.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	17563380	17564654	17563380	ID=MsG0480019274.01;Name=MsG0480019274.01
Chr4	mRNA	17563380	17564654	17563380	ID=MsG0480019274.01.T01;Parent=MsG0480019274.01;Name=MsG0480019274.01.T01;_AED=0.32;_eAED=0.32;_QI=0\|0\|0\|1\|0\|0\|2\|0\|218
Chr4	exon	17564248	17564654	17564248	ID=MsG0480019274.01.T01:exon:4595;Parent=MsG0480019274.01.T01
Chr4	CDS	17564248	17564654	17564248	ID=MsG0480019274.01.T01:cds;Parent=MsG0480019274.01.T01
Chr4	CDS	17563380	17563629	17563380	ID=MsG0480019274.01.T01:cds;Parent=MsG0480019274.01.T01
Chr4	mRNA	17563380	17564528	17563380	ID=MsG0480019274.01.T02;Parent=MsG0480019274.01;Name=MsG0480019274.01.T02;_AED=0.35;_eAED=0.36;_QI=0\|0\|0\|1\|0\|0\|2\|0\|176
Chr4	exon	17564248	17564528	17564248	ID=MsG0480019274.01.T02:exon:4597;Parent=MsG0480019274.01.T02
Chr4	CDS	17564248	17564528	17564248	ID=MsG0480019274.01.T02:cds;Parent=MsG0480019274.01.T02
Chr4	CDS	17563380	17563629	17563380	ID=MsG0480019274.01.T02:cds;Parent=MsG0480019274.01.T02
Chr4	mRNA	17563380	17564276	17563380	ID=MsG0480019274.01.T03;Parent=MsG0480019274.01;Name=MsG0480019274.01.T03;_AED=0.42;_eAED=0.43;_QI=0\|0\|0\|1\|0\|0\|2\|0\|92
Chr4	exon	17564248	17564276	17564248	ID=MsG0480019274.01.T03:exon:4598;Parent=MsG0480019274.01.T03
Chr4	CDS	17564248	17564276	17564248	ID=MsG0480019274.01.T03:cds;Parent=MsG0480019274.01.T03
Chr4	CDS	17563380	17563629	17563380	ID=MsG0480019274.01.T03:cds;Parent=MsG0480019274.01.T03
Chr4	mRNA	17563380	17563646	17563380	ID=MsG0480019274.01.T04;Parent=MsG0480019274.01;Name=MsG0480019274.01.T04;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|88
Chr4	exon	17563380	17563646	17563380	ID=MsG0480019274.01.T04:exon:4599;Parent=MsG0480019274.01.T04
Chr4	CDS	17563380	17563646	17563380	ID=MsG0480019274.01.T04:cds;Parent=MsG0480019274.01.T04
Chr4	mRNA	17564285	17564374	17564285	ID=MsG0480019274.01.T05;Parent=MsG0480019274.01;Name=MsG0480019274.01.T05;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|29
Chr4	exon	17564285	17564374	17564285	ID=MsG0480019274.01.T05:exon:4600;Parent=MsG0480019274.01.T05
Chr4	CDS	17564285	17564374	17564285	ID=MsG0480019274.01.T05:cds;Parent=MsG0480019274.01.T05

Gene Sequence

>MsG0480019274.01.T01

ATGGATTCTTCTTCTTTGACAATATCTCCAAGAAAACTACACTATGATTTATATTCCTATTCATACAAAGAAGATTCAAACACCCCATTAGTGATAAATGTTCTAGCTTCATTAATTGAGAGAAACATGGCAAGAGCACAAAGAATTGTGAAGAATTGTTCTTCAAGAGTTTTGTCCAAAGCAAGCACAAAAATCTTTGATTGTAGAGAGATTCCTGATTTAACCATTCAATCTTATTTAGAGAGAATTTTTAGATACACACGTGCTGGACCTTCGGTTTATGTTGTTGCTTATGTCTATATTGATCGGTTTTGCCAAAATAATCTTGGGTTTAGAATTAATTCAAGAAATGTTCATAGACTTCTCATAACAACTATTATGGTTGCTTCAAAGTATGTTGAAGATATGAATTTCAGAAATTCATACTTTGCAAAAGTTGGTGGTTTAACAACAAATGAATTGAATGAGTTGGAGCTTGAATTTCTTTTCATGATGAATTTCAAATTACATGTTAATGTGAGTGTATTTGAGAGCTATTGTTCCCATTTGGAGAGGGAAGTAAGCATTGGAGGAGGGTACCACATTGAGAGGACCTTAAGGTGTGCTGAAGAAATCAAAGAAAGAGGTCACACACAAATTGCACGTGTAATGTTGTAG

>MsG0480019274.01.T05

ATGTTGTTGCTTATGTCTATATTGATCGGTTTTGCCAAAATAATCTTGGGTTTAGAATTAATTCAAGAAATGTTCATAGACTTCTCATAA

>MsG0480019274.01.T04

TGCTTTTTATCTGGCAGGAATTTCAGAAATTCATACTTTGCAAAAGTTGGTGGTTTAACAACAAATGAATTGAATGAGTTGGAGCTTGAATTTCTTTTCATGATGAATTTCAAATTACATGTTAATGTGAGTGTATTTGAGAGCTATTGTTCCCATTTGGAGAGGGAAGTAAGCATTGGAGGAGGGTACCACATTGAGAGGACCTTAAGGTGTGCTGAAGAAATCAAAGAAAGAGGTCACACACAAATTGCACGTGTAATGTTGTAG

>MsG0480019274.01.T03

ATGGTTGCTTCAAAGTATGTTGAAGATATGAATTTCAGAAATTCATACTTTGCAAAAGTTGGTGGTTTAACAACAAATGAATTGAATGAGTTGGAGCTTGAATTTCTTTTCATGATGAATTTCAAATTACATGTTAATGTGAGTGTATTTGAGAGCTATTGTTCCCATTTGGAGAGGGAAGTAAGCATTGGAGGAGGGTACCACATTGAGAGGACCTTAAGGTGTGCTGAAGAAATCAAAGAAAGAGGTCACACACAAATTGCACGTGTAATGTTGTAG

>MsG0480019274.01.T02

ATGGCAAGAGCACAAAGAATTGTGAAGAATTGTTCTTCAAGAGTTTTGTCCAAAGCAAGCACAAAAATCTTTGATTGTAGAGAGATTCCTGATTTAACCATTCAATCTTATTTAGAGAGAATTTTTAGATACACACGTGCTGGACCTTCGGTTTATGTTGTTGCTTATGTCTATATTGATCGGTTTTGCCAAAATAATCTTGGGTTTAGAATTAATTCAAGAAATGTTCATAGACTTCTCATAACAACTATTATGGTTGCTTCAAAGTATGTTGAAGATATGAATTTCAGAAATTCATACTTTGCAAAAGTTGGTGGTTTAACAACAAATGAATTGAATGAGTTGGAGCTTGAATTTCTTTTCATGATGAATTTCAAATTACATGTTAATGTGAGTGTATTTGAGAGCTATTGTTCCCATTTGGAGAGGGAAGTAAGCATTGGAGGAGGGTACCACATTGAGAGGACCTTAAGGTGTGCTGAAGAAATCAAAGAAAGAGGTCACACACAAATTGCACGTGTAATGTTGTAG

Protein sequence

>MsG0480019274.01.T01

MDSSSLTISPRKLHYDLYSYSYKEDSNTPLVINVLASLIERNMARAQRIVKNCSSRVLSKASTKIFDCREIPDLTIQSYLERIFRYTRAGPSVYVVAYVYIDRFCQNNLGFRINSRNVHRLLITTIMVASKYVEDMNFRNSYFAKVGGLTTNELNELELEFLFMMNFKLHVNVSVFESYCSHLEREVSIGGGYHIERTLRCAEEIKERGHTQIARVML*

>MsG0480019274.01.T05

MLLLMSILIGFAKIILGLELIQEMFIDFS*

>MsG0480019274.01.T04

CFLSGRNFRNSYFAKVGGLTTNELNELELEFLFMMNFKLHVNVSVFESYCSHLEREVSIGGGYHIERTLRCAEEIKERGHTQIARVML*

>MsG0480019274.01.T03

MVASKYVEDMNFRNSYFAKVGGLTTNELNELELEFLFMMNFKLHVNVSVFESYCSHLEREVSIGGGYHIERTLRCAEEIKERGHTQIARVML*

>MsG0480019274.01.T02

MARAQRIVKNCSSRVLSKASTKIFDCREIPDLTIQSYLERIFRYTRAGPSVYVVAYVYIDRFCQNNLGFRINSRNVHRLLITTIMVASKYVEDMNFRNSYFAKVGGLTTNELNELELEFLFMMNFKLHVNVSVFESYCSHLEREVSIGGGYHIERTLRCAEEIKERGHTQIARVML*