Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019314.01.T01 | GAU26584.1 | 54.206 | 107 | 42 | 3 | 1 | 100 | 1 | 107 | 6.41E-14 | 73.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019314.01.T01 | A0A2Z6M219 | 54.206 | 107 | 42 | 3 | 1 | 100 | 1 | 107 | 3.06e-14 | 73.9 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480019314.01 | MsG0580027785.01 | 0.823288 | 1.501708e-53 | 1.287510e-50 |
| MsG0480019314.01 | MsG0580027788.01 | 0.811017 | 8.637512e-51 | 5.288192e-48 |
| MsG0480019314.01 | MsG0780036620.01 | 0.811951 | 5.415596e-51 | 3.398232e-48 |
| MsG0480019314.01 | MsG0880042086.01 | 0.810174 | 1.314051e-50 | 7.865976e-48 |
| MsG0480019314.01 | MsG0880042415.01 | 0.801123 | 1.044357e-48 | 4.948205e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019314.01.T01 | MTR_4g029620 | 46.729 | 107 | 50 | 3 | 1 | 100 | 1 | 107 | 4.10e-12 | 60.8 |
| MsG0480019314.01.T01 | MTR_4g029730 | 55.882 | 102 | 19 | 4 | 1 | 100 | 1 | 78 | 2.03e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGACATGTGGTTTCTTTAC+CGG | 0.286836 | 4:+18355316 | None:intergenic |
| CCTACTAACCCGCCATCTTA+TGG | 0.296857 | 4:-18355451 | MsG0480019314.01.T01:CDS |
| CCTCAGGGGCTTGCACAATA+TGG | 0.311396 | 4:-18355520 | MsG0480019314.01.T01:CDS |
| TTCATCGAAGGTCTGTATAC+TGG | 0.343057 | 4:+18355406 | None:intergenic |
| ATACAGACCTTCGATGAAAA+AGG | 0.344638 | 4:-18355401 | MsG0480019314.01.T01:CDS |
| GGATGACAATGAGAACAATA+AGG | 0.364805 | 4:+18355542 | None:intergenic |
| ACTGGTAACTTCTTAGTTGA+TGG | 0.391204 | 4:+18355424 | None:intergenic |
| TGTTCTCATTGTCATCCCTC+AGG | 0.409896 | 4:-18355536 | MsG0480019314.01.T01:CDS |
| CTTTACCGGCGGCTTGAAAA+CGG | 0.417985 | 4:+18355330 | None:intergenic |
| CCATAAGATGGCGGGTTAGT+AGG | 0.418019 | 4:+18355451 | None:intergenic |
| GTTCTCATTGTCATCCCTCA+GGG | 0.452595 | 4:-18355535 | MsG0480019314.01.T01:CDS |
| GATGACAATGAGAACAATAA+GGG | 0.456734 | 4:+18355543 | None:intergenic |
| AGAAGTTGATGGCGGGTTAG+TGG | 0.457626 | 4:+18355477 | None:intergenic |
| GCTTCTCAACCTGTGGTTTG+CGG | 0.467537 | 4:+18355296 | None:intergenic |
| GAAGTTGATGGCGGGTTAGT+GGG | 0.509060 | 4:+18355478 | None:intergenic |
| TTAGTAGGATAAGAAGTTGA+TGG | 0.518305 | 4:+18355466 | None:intergenic |
| ACGGAGGGCTTCTCAACTGA+TGG | 0.534124 | 4:+18355349 | None:intergenic |
| GTTGATGGCGGTCCATAAGA+TGG | 0.544968 | 4:+18355439 | None:intergenic |
| GAACAATAAGGGGCAACACT+AGG | 0.558445 | 4:+18355554 | None:intergenic |
| ATGGCGGTCCATAAGATGGC+GGG | 0.569210 | 4:+18355443 | None:intergenic |
| TTAGTGGGATAAGAAGTTGC+TGG | 0.570467 | 4:+18355493 | None:intergenic |
| TAGGATAAGAAGTTGATGGC+GGG | 0.570472 | 4:+18355470 | None:intergenic |
| GTAGGATAAGAAGTTGATGG+CGG | 0.573174 | 4:+18355469 | None:intergenic |
| TCAACTGATGGTTTGTACAC+CGG | 0.578953 | 4:+18355361 | None:intergenic |
| ACCTGTGGTTTGCGGACATG+TGG | 0.581430 | 4:+18355304 | None:intergenic |
| ACCACATGTCCGCAAACCAC+AGG | 0.582421 | 4:-18355305 | MsG0480019314.01.T01:CDS |
| TTCTCATTGTCATCCCTCAG+GGG | 0.587596 | 4:-18355534 | MsG0480019314.01.T01:CDS |
| CCATATTGTGCAAGCCCCTG+AGG | 0.593529 | 4:+18355520 | None:intergenic |
| TATCAAGGCTTCTCAACCTG+TGG | 0.599916 | 4:+18355289 | None:intergenic |
| CATATTGTGCAAGCCCCTGA+GGG | 0.605127 | 4:+18355521 | None:intergenic |
| TACCGGCGGCTTGAAAACGG+AGG | 0.617285 | 4:+18355333 | None:intergenic |
| ACCGGCGGCTTGAAAACGGA+GGG | 0.626111 | 4:+18355334 | None:intergenic |
| GGTAACTTCTTAGTTGATGG+CGG | 0.628583 | 4:+18355427 | None:intergenic |
| ACATGTGGTTTCTTTACCGG+CGG | 0.630847 | 4:+18355319 | None:intergenic |
| GGGCAACACTAGGAAGCGAA+AGG | 0.658929 | 4:+18355564 | None:intergenic |
| ATGACAATGAGAACAATAAG+GGG | 0.665224 | 4:+18355544 | None:intergenic |
| GATGGCGGTCCATAAGATGG+CGG | 0.675096 | 4:+18355442 | None:intergenic |
| GTGGGATAAGAAGTTGCTGG+CGG | 0.712239 | 4:+18355496 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGACAATGAGAACAATAAG+GGG | + | Chr4:18355321-18355340 | None:intergenic | 30.0% | |
| GATGACAATGAGAACAATAA+GGG | + | Chr4:18355322-18355341 | None:intergenic | 30.0% | |
| TTAGTAGGATAAGAAGTTGA+TGG | + | Chr4:18355399-18355418 | None:intergenic | 30.0% | |
| ACTGGTAACTTCTTAGTTGA+TGG | + | Chr4:18355441-18355460 | None:intergenic | 35.0% | |
| ATACAGACCTTCGATGAAAA+AGG | - | Chr4:18355461-18355480 | MsG0480019314.01.T01:CDS | 35.0% | |
| GGATGACAATGAGAACAATA+AGG | + | Chr4:18355323-18355342 | None:intergenic | 35.0% | |
| TTAGTGGGATAAGAAGTTGC+TGG | + | Chr4:18355372-18355391 | None:intergenic | 40.0% | |
| TTCATCGAAGGTCTGTATAC+TGG | + | Chr4:18355459-18355478 | None:intergenic | 40.0% | |
| ! | TCAACTGATGGTTTGTACAC+CGG | + | Chr4:18355504-18355523 | None:intergenic | 40.0% |
| !! | GGTAACTTCTTAGTTGATGG+CGG | + | Chr4:18355438-18355457 | None:intergenic | 40.0% |
| !! | GTAGGATAAGAAGTTGATGG+CGG | + | Chr4:18355396-18355415 | None:intergenic | 40.0% |
| !! | TACACCGGTGATTTTTCAAC+TGG | + | Chr4:18355489-18355508 | None:intergenic | 40.0% |
| !! | TAGGATAAGAAGTTGATGGC+GGG | + | Chr4:18355395-18355414 | None:intergenic | 40.0% |
| ACATGTGGTTTCTTTACCGG+CGG | + | Chr4:18355546-18355565 | None:intergenic | 45.0% | |
| CGGACATGTGGTTTCTTTAC+CGG | + | Chr4:18355549-18355568 | None:intergenic | 45.0% | |
| GAACAATAAGGGGCAACACT+AGG | + | Chr4:18355311-18355330 | None:intergenic | 45.0% | |
| GGTGCCAGTTGAAAAATCAC+CGG | - | Chr4:18355482-18355501 | MsG0480019314.01.T01:CDS | 45.0% | |
| GTTCTCATTGTCATCCCTCA+GGG | - | Chr4:18355327-18355346 | MsG0480019314.01.T01:CDS | 45.0% | |
| TGTTCTCATTGTCATCCCTC+AGG | - | Chr4:18355326-18355345 | MsG0480019314.01.T01:CDS | 45.0% | |
| TTCTCATTGTCATCCCTCAG+GGG | - | Chr4:18355328-18355347 | MsG0480019314.01.T01:CDS | 45.0% | |
| !! | ACTGGCACCTTTTTCATCGA+AGG | + | Chr4:18355471-18355490 | None:intergenic | 45.0% |
| CATATTGTGCAAGCCCCTGA+GGG | + | Chr4:18355344-18355363 | None:intergenic | 50.0% | |
| CCTACTAACCCGCCATCTTA+TGG | - | Chr4:18355411-18355430 | MsG0480019314.01.T01:CDS | 50.0% | |
| CTTTACCGGCGGCTTGAAAA+CGG | + | Chr4:18355535-18355554 | None:intergenic | 50.0% | |
| GCTTCTCAACCTGTGGTTTG+CGG | + | Chr4:18355569-18355588 | None:intergenic | 50.0% | |
| GTGGGATAAGAAGTTGCTGG+CGG | + | Chr4:18355369-18355388 | None:intergenic | 50.0% | |
| !! | AGAAGTTGATGGCGGGTTAG+TGG | + | Chr4:18355388-18355407 | None:intergenic | 50.0% |
| !! | CCATAAGATGGCGGGTTAGT+AGG | + | Chr4:18355414-18355433 | None:intergenic | 50.0% |
| !! | GAAGTTGATGGCGGGTTAGT+GGG | + | Chr4:18355387-18355406 | None:intergenic | 50.0% |
| !! | GTTGATGGCGGTCCATAAGA+TGG | + | Chr4:18355426-18355445 | None:intergenic | 50.0% |
| ACCACATGTCCGCAAACCAC+AGG | - | Chr4:18355557-18355576 | MsG0480019314.01.T01:CDS | 55.0% | |
| ACGGAGGGCTTCTCAACTGA+TGG | + | Chr4:18355516-18355535 | None:intergenic | 55.0% | |
| ATGGCGGTCCATAAGATGGC+GGG | + | Chr4:18355422-18355441 | None:intergenic | 55.0% | |
| CCATATTGTGCAAGCCCCTG+AGG | + | Chr4:18355345-18355364 | None:intergenic | 55.0% | |
| CCTCAGGGGCTTGCACAATA+TGG | - | Chr4:18355342-18355361 | MsG0480019314.01.T01:CDS | 55.0% | |
| GGGCAACACTAGGAAGCGAA+AGG | + | Chr4:18355301-18355320 | None:intergenic | 55.0% | |
| ! | ACCTGTGGTTTGCGGACATG+TGG | + | Chr4:18355561-18355580 | None:intergenic | 55.0% |
| ! | GATGGCGGTCCATAAGATGG+CGG | + | Chr4:18355423-18355442 | None:intergenic | 55.0% |
| ACCGGCGGCTTGAAAACGGA+GGG | + | Chr4:18355531-18355550 | None:intergenic | 60.0% | |
| TACCGGCGGCTTGAAAACGG+AGG | + | Chr4:18355532-18355551 | None:intergenic | 60.0% | |
| !! | GCCCTCCGTTTTCAAGCCGC+CGG | - | Chr4:18355527-18355546 | MsG0480019314.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 18355291 | 18355593 | 18355291 | ID=MsG0480019314.01;Name=MsG0480019314.01 |
| Chr4 | mRNA | 18355291 | 18355593 | 18355291 | ID=MsG0480019314.01.T01;Parent=MsG0480019314.01;Name=MsG0480019314.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|100 |
| Chr4 | exon | 18355291 | 18355593 | 18355291 | ID=MsG0480019314.01.T01:exon:6194;Parent=MsG0480019314.01.T01 |
| Chr4 | CDS | 18355291 | 18355593 | 18355291 | ID=MsG0480019314.01.T01:cds;Parent=MsG0480019314.01.T01 |
| Gene Sequence |
| Protein sequence |
