Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027788.01.T01 | GAU26584.1 | 56.701 | 97 | 36 | 2 | 1 | 91 | 1 | 97 | 2.70E-14 | 74.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027788.01.T01 | A0A2Z6M219 | 56.701 | 97 | 36 | 2 | 1 | 91 | 1 | 97 | 1.29e-14 | 74.7 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000040.01 | MsG0580027788.01 | 0.805490 | 1.301937e-49 | 6.897133e-47 |
| MsG0180005666.01 | MsG0580027788.01 | 0.825069 | 5.731382e-54 | 5.169624e-51 |
| MsG0480019314.01 | MsG0580027788.01 | 0.811017 | 8.637512e-51 | 5.288192e-48 |
| MsG0480019644.01 | MsG0580027788.01 | 0.804392 | 2.208355e-49 | 1.137266e-46 |
| MsG0580027785.01 | MsG0580027788.01 | 0.899991 | 1.144059e-77 | 1.449401e-73 |
| MsG0580027788.01 | MsG0780036620.01 | 0.812540 | 4.028134e-51 | 2.568148e-48 |
| MsG0580027788.01 | MsG0880042922.01 | 0.801393 | 9.197682e-49 | 4.387402e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027788.01.T01 | MTR_4g029730 | 60.215 | 93 | 20 | 2 | 1 | 91 | 1 | 78 | 2.85e-14 | 62.4 |
| MsG0580027788.01.T01 | MTR_4g029620 | 48.421 | 95 | 43 | 2 | 1 | 89 | 1 | 95 | 2.99e-14 | 66.6 |
| MsG0580027788.01.T01 | MTR_4g029550 | 53.922 | 102 | 36 | 2 | 1 | 91 | 1 | 102 | 9.39e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGACATGTGGTTTCTTTAC+CGG | 0.286836 | 5:-68485436 | None:intergenic |
| CCTACTAACCCGCCATCTTA+TGG | 0.296857 | 5:+68485301 | MsG0580027788.01.T01:CDS |
| CCTCAGGGGCTTGCACAATA+TGG | 0.311396 | 5:+68485259 | MsG0580027788.01.T01:CDS |
| GTACACACCTTCGATGAAAA+AGG | 0.359057 | 5:+68485351 | MsG0580027788.01.T01:CDS |
| GGATGACAATGAGAACAATA+AGG | 0.364805 | 5:-68485237 | None:intergenic |
| ACCGGTAACTTCTTAGTTGA+TGG | 0.388474 | 5:-68485328 | None:intergenic |
| TGTTCTCATTGTCATCCCTC+AGG | 0.409896 | 5:+68485243 | MsG0580027788.01.T01:CDS |
| CTTTACCGGCGGCTTGAAAA+CGG | 0.417985 | 5:-68485422 | None:intergenic |
| CCATAAGATGGCGGGTTAGT+AGG | 0.418019 | 5:-68485301 | None:intergenic |
| GTTCTCATTGTCATCCCTCA+GGG | 0.452595 | 5:+68485244 | MsG0580027788.01.T01:CDS |
| GATGACAATGAGAACAATAA+GGG | 0.456734 | 5:-68485236 | None:intergenic |
| GCTTCTCAACCTGTGGTTTG+CGG | 0.467537 | 5:-68485456 | None:intergenic |
| GCCATCAACTAAGAAGTTAC+CGG | 0.510455 | 5:+68485327 | MsG0580027788.01.T01:CDS |
| TTAGTAGGATAAGAAGTTGC+TGG | 0.519871 | 5:-68485286 | None:intergenic |
| ACGGAGGGCTTCTCAACTGA+TGG | 0.534124 | 5:-68485403 | None:intergenic |
| GTTGATGGCGGTCCATAAGA+TGG | 0.544968 | 5:-68485313 | None:intergenic |
| GAACAATAAGGGGCAACACT+AGG | 0.558445 | 5:-68485225 | None:intergenic |
| ATGGCGGTCCATAAGATGGC+GGG | 0.569210 | 5:-68485309 | None:intergenic |
| TCAACTGATGGTTTGTACAC+CGG | 0.578953 | 5:-68485391 | None:intergenic |
| ACCTGTGGTTTGCGGACATG+TGG | 0.581430 | 5:-68485448 | None:intergenic |
| ACCACATGTCCGCAAACCAC+AGG | 0.582421 | 5:+68485447 | MsG0580027788.01.T01:CDS |
| TTCTCATTGTCATCCCTCAG+GGG | 0.587596 | 5:+68485245 | MsG0580027788.01.T01:CDS |
| CCATATTGTGCAAGCCCCTG+AGG | 0.593529 | 5:-68485259 | None:intergenic |
| TTCATCGAAGGTGTGTACAC+CGG | 0.594888 | 5:-68485346 | None:intergenic |
| TATCAAGGCTTCTCAACCTG+TGG | 0.599916 | 5:-68485463 | None:intergenic |
| CATATTGTGCAAGCCCCTGA+GGG | 0.605127 | 5:-68485258 | None:intergenic |
| TACCGGCGGCTTGAAAACGG+AGG | 0.617285 | 5:-68485419 | None:intergenic |
| ACCGGCGGCTTGAAAACGGA+GGG | 0.626111 | 5:-68485418 | None:intergenic |
| ACATGTGGTTTCTTTACCGG+CGG | 0.630847 | 5:-68485433 | None:intergenic |
| GGTAACTTCTTAGTTGATGG+CGG | 0.653612 | 5:-68485325 | None:intergenic |
| GGGCAACACTAGGAAGCGAA+AGG | 0.658929 | 5:-68485215 | None:intergenic |
| ATGACAATGAGAACAATAAG+GGG | 0.665224 | 5:-68485235 | None:intergenic |
| GATGGCGGTCCATAAGATGG+CGG | 0.675096 | 5:-68485310 | None:intergenic |
| GTAGGATAAGAAGTTGCTGG+CGG | 0.732669 | 5:-68485283 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGACAATGAGAACAATAAG+GGG | - | Chr5:68485238-68485257 | None:intergenic | 30.0% | |
| GATGACAATGAGAACAATAA+GGG | - | Chr5:68485239-68485258 | None:intergenic | 30.0% | |
| GGATGACAATGAGAACAATA+AGG | - | Chr5:68485240-68485259 | None:intergenic | 35.0% | |
| TTAGTAGGATAAGAAGTTGC+TGG | - | Chr5:68485289-68485308 | None:intergenic | 35.0% | |
| ACCGGTAACTTCTTAGTTGA+TGG | - | Chr5:68485331-68485350 | None:intergenic | 40.0% | |
| GCCATCAACTAAGAAGTTAC+CGG | + | Chr5:68485327-68485346 | MsG0580027788.01.T01:CDS | 40.0% | |
| GTACACACCTTCGATGAAAA+AGG | + | Chr5:68485351-68485370 | MsG0580027788.01.T01:CDS | 40.0% | |
| ! | TCAACTGATGGTTTGTACAC+CGG | - | Chr5:68485394-68485413 | None:intergenic | 40.0% |
| !! | GGTAACTTCTTAGTTGATGG+CGG | - | Chr5:68485328-68485347 | None:intergenic | 40.0% |
| !! | TACACCGGTGATTTTTCAAC+TGG | - | Chr5:68485379-68485398 | None:intergenic | 40.0% |
| ACATGTGGTTTCTTTACCGG+CGG | - | Chr5:68485436-68485455 | None:intergenic | 45.0% | |
| CGGACATGTGGTTTCTTTAC+CGG | - | Chr5:68485439-68485458 | None:intergenic | 45.0% | |
| GAACAATAAGGGGCAACACT+AGG | - | Chr5:68485228-68485247 | None:intergenic | 45.0% | |
| GGTGCCAGTTGAAAAATCAC+CGG | + | Chr5:68485372-68485391 | MsG0580027788.01.T01:CDS | 45.0% | |
| GTAGGATAAGAAGTTGCTGG+CGG | - | Chr5:68485286-68485305 | None:intergenic | 45.0% | |
| GTTCTCATTGTCATCCCTCA+GGG | + | Chr5:68485244-68485263 | MsG0580027788.01.T01:CDS | 45.0% | |
| TGTTCTCATTGTCATCCCTC+AGG | + | Chr5:68485243-68485262 | MsG0580027788.01.T01:CDS | 45.0% | |
| TTCTCATTGTCATCCCTCAG+GGG | + | Chr5:68485245-68485264 | MsG0580027788.01.T01:CDS | 45.0% | |
| ! | TTCATCGAAGGTGTGTACAC+CGG | - | Chr5:68485349-68485368 | None:intergenic | 45.0% |
| !! | ACTGGCACCTTTTTCATCGA+AGG | - | Chr5:68485361-68485380 | None:intergenic | 45.0% |
| CATATTGTGCAAGCCCCTGA+GGG | - | Chr5:68485261-68485280 | None:intergenic | 50.0% | |
| CCTACTAACCCGCCATCTTA+TGG | + | Chr5:68485301-68485320 | MsG0580027788.01.T01:CDS | 50.0% | |
| CTTTACCGGCGGCTTGAAAA+CGG | - | Chr5:68485425-68485444 | None:intergenic | 50.0% | |
| GCTTCTCAACCTGTGGTTTG+CGG | - | Chr5:68485459-68485478 | None:intergenic | 50.0% | |
| !! | CCATAAGATGGCGGGTTAGT+AGG | - | Chr5:68485304-68485323 | None:intergenic | 50.0% |
| !! | GTTGATGGCGGTCCATAAGA+TGG | - | Chr5:68485316-68485335 | None:intergenic | 50.0% |
| ACCACATGTCCGCAAACCAC+AGG | + | Chr5:68485447-68485466 | MsG0580027788.01.T01:CDS | 55.0% | |
| ACGGAGGGCTTCTCAACTGA+TGG | - | Chr5:68485406-68485425 | None:intergenic | 55.0% | |
| ATGGCGGTCCATAAGATGGC+GGG | - | Chr5:68485312-68485331 | None:intergenic | 55.0% | |
| CCATATTGTGCAAGCCCCTG+AGG | - | Chr5:68485262-68485281 | None:intergenic | 55.0% | |
| CCTCAGGGGCTTGCACAATA+TGG | + | Chr5:68485259-68485278 | MsG0580027788.01.T01:CDS | 55.0% | |
| GGGCAACACTAGGAAGCGAA+AGG | - | Chr5:68485218-68485237 | None:intergenic | 55.0% | |
| ! | ACCTGTGGTTTGCGGACATG+TGG | - | Chr5:68485451-68485470 | None:intergenic | 55.0% |
| ! | GATGGCGGTCCATAAGATGG+CGG | - | Chr5:68485313-68485332 | None:intergenic | 55.0% |
| ACCGGCGGCTTGAAAACGGA+GGG | - | Chr5:68485421-68485440 | None:intergenic | 60.0% | |
| TACCGGCGGCTTGAAAACGG+AGG | - | Chr5:68485422-68485441 | None:intergenic | 60.0% | |
| !! | GCCCTCCGTTTTCAAGCCGC+CGG | + | Chr5:68485417-68485436 | MsG0580027788.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 68485208 | 68485483 | 68485208 | ID=MsG0580027788.01;Name=MsG0580027788.01 |
| Chr5 | mRNA | 68485208 | 68485483 | 68485208 | ID=MsG0580027788.01.T01;Parent=MsG0580027788.01;Name=MsG0580027788.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|91 |
| Chr5 | exon | 68485208 | 68485483 | 68485208 | ID=MsG0580027788.01.T01:exon:14354;Parent=MsG0580027788.01.T01 |
| Chr5 | CDS | 68485208 | 68485483 | 68485208 | ID=MsG0580027788.01.T01:cds;Parent=MsG0580027788.01.T01 |
| Gene Sequence |
| Protein sequence |
