AlfalfaGEDB | MsG0480019542.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019542.01

KEGG: K03065
GO: GO:0005524
PFAM: PF00004

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019542.01.T01	KAG7021077.1	97.305	334	9	0	1	334	1	334	0	661

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019542.01.T01	P33297	54.847	392	108	6	13	335	19	410	9.86E-135	393

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019542.01.T01	A0A835JEV5	95.522	335	15	0	1	335	1	335	0.0	651

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480019542.01	MsG0880042524.01	0.810748	9.880344e-51	6.005291e-48

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PPI

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Gene1	Gene2	Type
MsG0180003744.01	MsG0480019542.01	PPI
MsG0180005113.01	MsG0480019542.01	PPI
MsG0180005153.01	MsG0480019542.01	PPI
MsG0180005236.01	MsG0480019542.01	PPI
MsG0280008911.01	MsG0480019542.01	PPI
MsG0280009575.01	MsG0480019542.01	PPI
MsG0280009575.01	MsG0480019542.01	PPI
MsG0380015066.01	MsG0480019542.01	PPI
MsG0380017800.01	MsG0480019542.01	PPI
MsG0480019542.01	MsG0880042848.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019542.01.T01	MTR_1g083330	98.324	179	3	0	1	179	1	179	6.91e-120	352
MsG0480019542.01.T01	MTR_1g083330	100.000	158	0	0	178	335	242	399	1.57e-109	326
MsG0480019542.01.T01	MTR_4g033235	97.753	178	4	0	2	179	3	180	2.39e-119	351
MsG0480019542.01.T01	MTR_4g033235	100.000	158	0	0	178	335	243	400	1.97e-109	326
MsG0480019542.01.T01	MTR_8g023550	41.637	281	109	3	120	345	115	395	3.01e-64	209
MsG0480019542.01.T01	MTR_1g069105	38.663	344	152	8	37	329	79	414	9.32e-60	199
MsG0480019542.01.T01	MTR_7g099640	38.372	344	153	8	37	329	78	413	4.36e-59	196
MsG0480019542.01.T01	MTR_7g099640	38.372	344	153	8	37	329	78	413	6.34e-59	196
MsG0480019542.01.T01	MTR_4g094270	56.250	160	69	1	178	336	231	390	6.68e-57	191
MsG0480019542.01.T01	MTR_3g112260	54.000	150	69	0	180	329	213	362	5.83e-54	182

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019542.01.T01	AT3G05530	90.556	180	16	1	1	179	1	180	8.86e-110	327
MsG0480019542.01.T01	AT3G05530	98.734	158	2	0	178	335	243	400	2.20e-108	323
MsG0480019542.01.T01	AT1G09100	91.061	179	16	0	1	179	1	179	1.82e-109	326
MsG0480019542.01.T01	AT1G09100	97.468	158	4	0	178	335	242	399	2.61e-105	315
MsG0480019542.01.T01	AT4G29040	35.854	357	144	5	37	329	76	411	1.50e-60	201
MsG0480019542.01.T01	AT2G20140	35.013	377	157	6	37	346	76	431	1.67e-60	201
MsG0480019542.01.T01	AT5G19990	56.875	160	68	1	178	336	175	334	1.11e-57	191
MsG0480019542.01.T01	AT5G20000	56.875	160	68	1	178	336	233	392	3.80e-57	191
MsG0480019542.01.T01	AT5G19990	56.875	160	68	1	178	336	233	392	3.96e-57	191
MsG0480019542.01.T01	AT5G19990	56.875	160	68	1	178	336	233	392	3.96e-57	191

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Find 118 sgRNAs with CRISPR-Local

Find 328 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGAAACTAAGTTGTTG+GGG	0.547574	4:-21882692	None:intergenic
AAAAGGTTGGACATTCGTCC+TGG	0.527602	4:+21882660	MsG0480019542.01.T01:intron
AAACAATTACCCTATCTCGT+TGG	0.607535	4:+21879558	MsG0480019542.01.T01:CDS
AAACGAAACTAAGTTGTTGG+GGG	0.689586	4:-21882691	None:intergenic
AAACGACTATAATTTAGAGA+CGG	0.633680	4:-21883005	None:intergenic
AAAGCCGTCTGTGTTGAGGC+TGG	0.560514	4:+21882459	MsG0480019542.01.T01:CDS
AACAAACCTCAGTTGCATCA+CGG	0.628397	4:-21882497	None:intergenic
AACAATGTTACCAACGAGAT+AGG	0.403098	4:-21879568	None:intergenic
AACCACGAGGACTTTAATGA+AGG	0.588663	4:+21883650	MsG0480019542.01.T01:three_prime_UTR
AAGGAAATTCCCACATCAAC+AGG	0.416237	4:-21883598	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACAAAGTTATCACTCAC+TGG	-	Chr4:21880593-21880612	None:intergenic	30.0%
AAAACGAAACTAAGTTGTTG+GGG	-	Chr4:21882695-21882714	None:intergenic	30.0%
AAGGGGTTATTTAAGCTTTA+GGG	+	Chr4:21879875-21879894	MsG0480019542.01.T01:intron	30.0%
AATGAGGGCAAATAAAATAG+AGG	-	Chr4:21881431-21881450	None:intergenic	30.0%
AATTCTGTACAGATGTTCAT+AGG	+	Chr4:21881510-21881529	MsG0480019542.01.T01:intron	30.0%
AGAGAAGATTAAAGAGAATC+AGG	+	Chr4:21879518-21879537	MsG0480019542.01.T01:CDS	30.0%
AGTTCTTCAAAGTTTACATC+TGG	-	Chr4:21882402-21882421	None:intergenic	30.0%
ATACCATTTCATCATGTTTG+GGG	+	Chr4:21880221-21880240	MsG0480019542.01.T01:intron	30.0%
ATAGATGAAATCGATGCAAT+TGG	+	Chr4:21881600-21881619	MsG0480019542.01.T01:CDS	30.0%
ATGAGGGCAAATAAAATAGA+GGG	-	Chr4:21881430-21881449	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	21878992	21883811	21878992	ID=MsG0480019542.01;Name=MsG0480019542.01
Chr4	mRNA	21878992	21883811	21878992	ID=MsG0480019542.01.T01;Parent=MsG0480019542.01;Name=MsG0480019542.01.T01;_AED=0.46;_eAED=0.46;_QI=240\|0.90\|1\|1\|0.63\|0.5\|12\|841\|348
Chr4	exon	21878992	21879108	21878992	ID=MsG0480019542.01.T01:exon:10451;Parent=MsG0480019542.01.T01
Chr4	exon	21879146	21879385	21879146	ID=MsG0480019542.01.T01:exon:10452;Parent=MsG0480019542.01.T01
Chr4	exon	21879477	21879593	21879477	ID=MsG0480019542.01.T01:exon:10453;Parent=MsG0480019542.01.T01
Chr4	exon	21880102	21880209	21880102	ID=MsG0480019542.01.T01:exon:10454;Parent=MsG0480019542.01.T01
Chr4	exon	21880346	21880537	21880346	ID=MsG0480019542.01.T01:exon:10455;Parent=MsG0480019542.01.T01
Chr4	exon	21881522	21881640	21881522	ID=MsG0480019542.01.T01:exon:10456;Parent=MsG0480019542.01.T01
Chr4	exon	21881806	21881893	21881806	ID=MsG0480019542.01.T01:exon:10457;Parent=MsG0480019542.01.T01
Chr4	exon	21882160	21882279	21882160	ID=MsG0480019542.01.T01:exon:10458;Parent=MsG0480019542.01.T01
Chr4	exon	21882372	21882642	21882372	ID=MsG0480019542.01.T01:exon:10459;Parent=MsG0480019542.01.T01
Chr4	exon	21882665	21882761	21882665	ID=MsG0480019542.01.T01:exon:10460;Parent=MsG0480019542.01.T01
Chr4	exon	21882822	21883078	21882822	ID=MsG0480019542.01.T01:exon:10461;Parent=MsG0480019542.01.T01
Chr4	exon	21883410	21883811	21883410	ID=MsG0480019542.01.T01:exon:10462;Parent=MsG0480019542.01.T01
Chr4	five_prime_UTR	21878992	21879108	21878992	ID=MsG0480019542.01.T01:five_prime_utr;Parent=MsG0480019542.01.T01
Chr4	five_prime_UTR	21879146	21879268	21879146	ID=MsG0480019542.01.T01:five_prime_utr;Parent=MsG0480019542.01.T01
Chr4	CDS	21879269	21879385	21879269	ID=MsG0480019542.01.T01:cds;Parent=MsG0480019542.01.T01
Chr4	CDS	21879477	21879593	21879477	ID=MsG0480019542.01.T01:cds;Parent=MsG0480019542.01.T01
Chr4	CDS	21880102	21880209	21880102	ID=MsG0480019542.01.T01:cds;Parent=MsG0480019542.01.T01
Chr4	CDS	21880346	21880537	21880346	ID=MsG0480019542.01.T01:cds;Parent=MsG0480019542.01.T01
Chr4	CDS	21881522	21881640	21881522	ID=MsG0480019542.01.T01:cds;Parent=MsG0480019542.01.T01
Chr4	CDS	21881806	21881893	21881806	ID=MsG0480019542.01.T01:cds;Parent=MsG0480019542.01.T01
Chr4	CDS	21882160	21882279	21882160	ID=MsG0480019542.01.T01:cds;Parent=MsG0480019542.01.T01
Chr4	CDS	21882372	21882557	21882372	ID=MsG0480019542.01.T01:cds;Parent=MsG0480019542.01.T01
Chr4	three_prime_UTR	21882558	21882642	21882558	ID=MsG0480019542.01.T01:three_prime_utr;Parent=MsG0480019542.01.T01
Chr4	three_prime_UTR	21882665	21882761	21882665	ID=MsG0480019542.01.T01:three_prime_utr;Parent=MsG0480019542.01.T01
Chr4	three_prime_UTR	21882822	21883078	21882822	ID=MsG0480019542.01.T01:three_prime_utr;Parent=MsG0480019542.01.T01
Chr4	three_prime_UTR	21883410	21883811	21883410	ID=MsG0480019542.01.T01:three_prime_utr;Parent=MsG0480019542.01.T01

Gene Sequence

>MsG0480019542.01.T01

ATGGCGAGTGCAATGGTAGAAGATGGTAACTTCGAAGATGACCAATTAGCTAATATGAGCACCGATGATATCGTCAGAGCTTCTCGTCTTCTCGACAACGAGATTCGCATCCTCAAGGAAGAGTTGCAGAGGACGAATCTGGAACTTGAATCGTATAAAGAGAAGATTAAAGAGAATCAGGAGAAAATTAAGCTTAATAAACAATTACCCTATCTCGTTGGTAACATTGTTGAGATATTGGAGATGAATCCAGAAGATGAGGCTGAGGAAGATGGTGCTAATATTGACCTTGACTCACAAAGGAAGGGAAAATGTGTTGTGCTAAAAACATCTACTCGACAGACGATATTTCTTCCAGTTGTTGGACTTGTTGACCCTGACAAGCTAAAACCTGGTGATCTTGTTGGTGTAAACAAAGATAGTTACTTAATCTTGGATACTCTGCCTTCTGAGTATGATTCTAGAGTTAAGGCCATGGAAGTTGATGAAAAGCCAACAGAGGACTACAATGACATTGGTGGATTAGAGAAGCAGATGTTCATAGGAGATGGAGCAAAACTTGTTCGTGACGCCTTCCAACTTGCCAAAGAGAAGTCACCATGCATTATATTTATAGATGAAATCGATGCAATTGGTACAAAGCGTTTTGATAGTGAAGTGAGTGGGGACAGGGAGGTTCAAAGAACAATGTTAGAGTTGCTCAATCAGCTTGATGGTTTTAGCAGCGACGACCGGATTAAGGTGATAGCAGCAACAAATCGTGCAGATATTCTTGATCCTGCCCTCATGCGTTCTGGTCGATTGGACCGCAAGATTGAGTTTCCTCACCCAACTGAAGAAGCAAGAGCTAGAATTCTGCAGATCCATTCAAGGAAGATGAATGTTCACCCAGATGTAAACTTTGAAGAACTCGCTCGGTCCACAGATGATTTCAACGGTGCACAACTGAAAGCCGTCTGTGTTGAGGCTGGCATGCTGGCTCTGCGCCGTGATGCAACTGAGGTTTGTTTTCTGTCAGATTTGGAACAACTAATTTATACACCCTGA

Protein sequence

>MsG0480019542.01.T01

MASAMVEDGNFEDDQLANMSTDDIVRASRLLDNEIRILKEELQRTNLELESYKEKIKENQEKIKLNKQLPYLVGNIVEILEMNPEDEAEEDGANIDLDSQRKGKCVVLKTSTRQTIFLPVVGLVDPDKLKPGDLVGVNKDSYLILDTLPSEYDSRVKAMEVDEKPTEDYNDIGGLEKQMFIGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFDSEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRKIEFPHPTEEARARILQIHSRKMNVHPDVNFEELARSTDDFNGAQLKAVCVEAGMLALRRDATEVCFLSDLEQLIYTP*