Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022678.01.T01 | AFK34159.1 | 98.933 | 375 | 3 | 1 | 1 | 374 | 1 | 375 | 0 | 756 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0480022678.01 | 0.866411 | 2.908758e-65 | 9.794126e-62 |
| MsG0080047912.01 | MsG0480022678.01 | 0.815486 | 9.018562e-52 | 6.226557e-49 |
| MsG0080048058.01 | MsG0480022678.01 | 0.867928 | 9.528091e-66 | 3.384429e-62 |
| MsG0080048178.01 | MsG0480022678.01 | 0.802865 | 4.580472e-49 | 2.268302e-46 |
| MsG0080048383.01 | MsG0480022678.01 | 0.879208 | 1.503103e-69 | 8.080107e-66 |
| MsG0080048602.01 | MsG0480022678.01 | 0.813517 | 2.458917e-51 | 1.609261e-48 |
| MsG0080048687.01 | MsG0480022678.01 | 0.832854 | 7.454639e-56 | 8.436001e-53 |
| MsG0080048765.01 | MsG0480022678.01 | 0.863616 | 2.192325e-64 | 6.687590e-61 |
| MsG0080048770.01 | MsG0480022678.01 | 0.809535 | 1.803388e-50 | 1.061582e-47 |
| MsG0080048830.01 | MsG0480022678.01 | 0.824050 | 9.956524e-54 | 8.721722e-51 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022678.01.T01 | MTR_4g094270 | 98.933 | 375 | 3 | 1 | 1 | 374 | 1 | 375 | 0.0 | 756 |
| MsG0480022678.01.T01 | MTR_3g112260 | 47.892 | 332 | 157 | 3 | 58 | 374 | 24 | 354 | 2.27e-100 | 303 |
| MsG0480022678.01.T01 | MTR_1g095660 | 51.077 | 325 | 141 | 7 | 61 | 374 | 25 | 342 | 8.15e-100 | 301 |
| MsG0480022678.01.T01 | MTR_3g117230 | 46.450 | 338 | 172 | 3 | 45 | 374 | 21 | 357 | 1.14e-99 | 301 |
| MsG0480022678.01.T01 | MTR_7g099640 | 45.954 | 346 | 181 | 3 | 30 | 374 | 65 | 405 | 5.65e-99 | 300 |
| MsG0480022678.01.T01 | MTR_7g099640 | 45.954 | 346 | 181 | 3 | 30 | 374 | 65 | 405 | 1.05e-98 | 300 |
| MsG0480022678.01.T01 | MTR_1g069105 | 50.512 | 293 | 143 | 2 | 83 | 374 | 115 | 406 | 5.04e-98 | 298 |
| MsG0480022678.01.T01 | MTR_1g083330 | 53.435 | 262 | 120 | 2 | 113 | 373 | 124 | 384 | 7.18e-95 | 290 |
| MsG0480022678.01.T01 | MTR_4g033235 | 53.435 | 262 | 120 | 2 | 113 | 373 | 125 | 385 | 1.14e-94 | 289 |
| MsG0480022678.01.T01 | MTR_3g062510 | 44.767 | 344 | 176 | 3 | 31 | 373 | 46 | 376 | 1.34e-94 | 289 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022678.01.T01 | AT5G19990 | 93.103 | 377 | 23 | 2 | 1 | 374 | 1 | 377 | 0.0 | 704 |
| MsG0480022678.01.T01 | AT5G19990 | 93.103 | 377 | 23 | 2 | 1 | 374 | 1 | 377 | 0.0 | 704 |
| MsG0480022678.01.T01 | AT5G20000 | 92.042 | 377 | 27 | 2 | 1 | 374 | 1 | 377 | 0.0 | 698 |
| MsG0480022678.01.T01 | AT5G19990 | 98.387 | 310 | 4 | 1 | 66 | 374 | 10 | 319 | 0.0 | 618 |
| MsG0480022678.01.T01 | AT1G53750 | 52.665 | 319 | 139 | 5 | 61 | 374 | 72 | 383 | 2.78e-105 | 317 |
| MsG0480022678.01.T01 | AT5G43010 | 48.494 | 332 | 155 | 3 | 58 | 374 | 24 | 354 | 2.94e-101 | 305 |
| MsG0480022678.01.T01 | AT1G53780 | 52.516 | 318 | 139 | 4 | 61 | 373 | 243 | 553 | 1.85e-100 | 310 |
| MsG0480022678.01.T01 | AT1G53780 | 52.516 | 318 | 139 | 4 | 61 | 373 | 244 | 554 | 1.94e-100 | 310 |
| MsG0480022678.01.T01 | AT1G53780 | 52.516 | 318 | 139 | 4 | 61 | 373 | 244 | 554 | 1.94e-100 | 310 |
| MsG0480022678.01.T01 | AT1G53780 | 52.516 | 318 | 139 | 4 | 61 | 373 | 265 | 575 | 3.64e-100 | 310 |
Find 181 sgRNAs with CRISPR-Local
Find 386 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCTCAGGATGTTTAAT+TGG | 0.236938 | 4:+75387418 | None:intergenic |
| AAAATTTACCTTGATCTTAT+TGG | 0.110746 | 4:+75386580 | None:intergenic |
| AAACAAAGTGATTGCATAAT+GGG | 0.288235 | 4:+75385885 | None:intergenic |
| AAACCAACCAAACAAGGGGA+GGG | 0.659041 | 4:-75390034 | MsG0480022678.01.T01:CDS |
| AAATCCTTCTTCCTCTTCTT+CGG | 0.384494 | 4:+75390305 | None:intergenic |
| AACATTGTTGGCTAGGGCAG+TGG | 0.488091 | 4:-75387236 | MsG0480022678.01.T01:CDS |
| AACGGGGAACTAAACATGGG+TGG | 0.635233 | 4:-75385584 | MsG0480022678.01.T01:three_prime_UTR |
| AACGTAACGAGCTCAATTCT+AGG | 0.412070 | 4:-75389925 | MsG0480022678.01.T01:CDS |
| AACTGGGTGTAATAGTAACG+GGG | 0.765292 | 4:-75385600 | MsG0480022678.01.T01:three_prime_UTR |
| AAGATGCAAACCTCTTCATT+AGG | 0.374465 | 4:+75386293 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAACAAATTGAGCCATCA+TGG | + | Chr4:75386545-75386564 | None:intergenic | 30.0% | |
| AAAAGCTCAGGATGTTTAAT+TGG | + | Chr4:75387836-75387855 | None:intergenic | 30.0% | |
| AAAATGATTAGAGGACATTG+TGG | - | Chr4:75385606-75385625 | MsG0480022678.01.T01:three_prime_UTR | 30.0% | |
| AAACAAACAAAAGCTTATCC+TGG | + | Chr4:75385825-75385844 | None:intergenic | 30.0% | |
| AAATAGTACAGAATCTTGGT+TGG | - | Chr4:75387287-75387306 | MsG0480022678.01.T01:intron | 30.0% | |
| AACTCATGCAATATTACCTT+CGG | + | Chr4:75387881-75387900 | None:intergenic | 30.0% | |
| AATAGTAGGATTTGTGAAAG+GGG | + | Chr4:75389928-75389947 | None:intergenic | 30.0% | |
| AATATGGAATTCAACAAGGT+TGG | - | Chr4:75385653-75385672 | MsG0480022678.01.T01:three_prime_UTR | 30.0% | |
| ACATTTACTTCAAATGAGCA+TGG | + | Chr4:75388455-75388474 | None:intergenic | 30.0% | |
| ACTTTCATCAGATTAACCAA+TGG | + | Chr4:75387626-75387645 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 75384877 | 75390396 | 75384877 | ID=MsG0480022678.01;Name=MsG0480022678.01 |
| Chr4 | mRNA | 75384877 | 75390396 | 75384877 | ID=MsG0480022678.01.T01;Parent=MsG0480022678.01;Name=MsG0480022678.01.T01;_AED=0.44;_eAED=0.44;_QI=134|1|1|1|0.87|0.77|9|1189|374 |
| Chr4 | exon | 75384877 | 75386188 | 75384877 | ID=MsG0480022678.01.T01:exon:5628;Parent=MsG0480022678.01.T01 |
| Chr4 | exon | 75386304 | 75386402 | 75386304 | ID=MsG0480022678.01.T01:exon:5627;Parent=MsG0480022678.01.T01 |
| Chr4 | exon | 75386589 | 75386745 | 75386589 | ID=MsG0480022678.01.T01:exon:5626;Parent=MsG0480022678.01.T01 |
| Chr4 | exon | 75387130 | 75387293 | 75387130 | ID=MsG0480022678.01.T01:exon:5625;Parent=MsG0480022678.01.T01 |
| Chr4 | exon | 75387390 | 75387452 | 75387390 | ID=MsG0480022678.01.T01:exon:5624;Parent=MsG0480022678.01.T01 |
| Chr4 | exon | 75387558 | 75387782 | 75387558 | ID=MsG0480022678.01.T01:exon:5623;Parent=MsG0480022678.01.T01 |
| Chr4 | exon | 75388774 | 75388871 | 75388774 | ID=MsG0480022678.01.T01:exon:5622;Parent=MsG0480022678.01.T01 |
| Chr4 | exon | 75389924 | 75390141 | 75389924 | ID=MsG0480022678.01.T01:exon:5621;Parent=MsG0480022678.01.T01 |
| Chr4 | exon | 75390285 | 75390396 | 75390285 | ID=MsG0480022678.01.T01:exon:5620;Parent=MsG0480022678.01.T01 |
| Chr4 | five_prime_UTR | 75390285 | 75390396 | 75390285 | ID=MsG0480022678.01.T01:five_prime_utr;Parent=MsG0480022678.01.T01 |
| Chr4 | five_prime_UTR | 75390120 | 75390141 | 75390120 | ID=MsG0480022678.01.T01:five_prime_utr;Parent=MsG0480022678.01.T01 |
| Chr4 | CDS | 75389924 | 75390119 | 75389924 | ID=MsG0480022678.01.T01:cds;Parent=MsG0480022678.01.T01 |
| Chr4 | CDS | 75388774 | 75388871 | 75388774 | ID=MsG0480022678.01.T01:cds;Parent=MsG0480022678.01.T01 |
| Chr4 | CDS | 75387558 | 75387782 | 75387558 | ID=MsG0480022678.01.T01:cds;Parent=MsG0480022678.01.T01 |
| Chr4 | CDS | 75387390 | 75387452 | 75387390 | ID=MsG0480022678.01.T01:cds;Parent=MsG0480022678.01.T01 |
| Chr4 | CDS | 75387130 | 75387293 | 75387130 | ID=MsG0480022678.01.T01:cds;Parent=MsG0480022678.01.T01 |
| Chr4 | CDS | 75386589 | 75386745 | 75386589 | ID=MsG0480022678.01.T01:cds;Parent=MsG0480022678.01.T01 |
| Chr4 | CDS | 75386304 | 75386402 | 75386304 | ID=MsG0480022678.01.T01:cds;Parent=MsG0480022678.01.T01 |
| Chr4 | CDS | 75386066 | 75386188 | 75386066 | ID=MsG0480022678.01.T01:cds;Parent=MsG0480022678.01.T01 |
| Chr4 | three_prime_UTR | 75384877 | 75386065 | 75384877 | ID=MsG0480022678.01.T01:three_prime_utr;Parent=MsG0480022678.01.T01 |
| Gene Sequence |
>MsG0480022678.01.T01 ATGGCTGCCGTCGGAGTAGAAGTGAAGCATGCCGAGGGTGCACCGGAGGAGCATTGCTCTGCCAAACCAACCAAACAAGGGGAGGGTCTCCGTCAATACTATACTCAGCACATCCATGAGCTTCAGCTCCTTCACCGTCAAAAGAATCATAATCTTAACCGTCTCGAGGCGCAACGTAACGAGCTCAATTCTAGGGTGAGGATGCTTCGGGAAGAACTGCAGCTTCTTCAGGAGCCTGGTTCATATGTTGGTGAAGTCGTTAAAGTGATGGGCAAGAACAAAGTCTTGGTCAAGGTCCATCCAGAAGGGAAATATGTTGTTGATATTGATAAAAATATTGACATCACAAAGATTACACCATCTACTAGAGTTGCTCTTCGCAACGACAGTTATGTGCTTCATTTAGTTCTTCCAAGCAAAGTTGATCCATTGGTTAATCTGATGAAAGTTGAGAAAGTTCCTGATTCTACATATGACATGATTGGTGGTTTAGACCAACAAATCAAAGAAATAAAAGAGGTCATTGAGCTTCCAATTAAACATCCTGAGCTTTTTGAAAGTCTTGGAATAGCTCAACCGAAGGGTGTCTTGCTCTATGGGCCACCTGGTACAGGAAAAACATTGTTGGCTAGGGCAGTGGCACATCATACTGATTGTACATTCATCAGGGTGTCTGGTTCTGAACTTGTTCAGAAATACATTGGAGAAGGTTCTAGAATGGTTAGGGAACTTTTTGTTATGGCCAGGGAGCATGCTCCATCAATTATCTTCATGGACGAAATTGACAGTATTGGATCTGCTCGTATGGAATCTGGAAGTGGCAATGGTGATAGTGAAGTGCAGCGTACTATGCTGGAACTTCTCAACCAGTTGGATGGATTTGAAGCTTCCAATAAGATCAAGGTTTTGATGGCCACCAATCGGATTGATATCCTGGATCAAGCACTCCTGAGACCTGGACGGATTGACAGGAAGATTGAATTTCCAAATCCTAATGAAGAGTCTCGCCGAGATATCTTGAAAATCCATTCAAGAAGAATGAATTTAATGCGTGGTATTGACTTGAAGAAGATTGCTGAGAAGATGAATGGAGCATCTGGTGCTGAGCTTAAGGTATGTTCTTAA |
| Protein sequence |
>MsG0480022678.01.T01 MAAVGVEVKHAEGAPEEHCSAKPTKQGEGLRQYYTQHIHELQLLHRQKNHNLNRLEAQRNELNSRVRMLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKNIDITKITPSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNGDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRRDILKIHSRRMNLMRGIDLKKIAEKMNGASGAELKVCS* |
