AlfalfaGEDB | MsG0480019893.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019893.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019893.01.T01	RHN60007.1	89.017	173	7	1	1	161	4	176	2.78E-101	300

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019893.01.T01	Q9LRR4	34.637	179	99	2	5	166	8	185	2.43E-26	107

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019893.01.T01	A0A396IAZ5	89.017	173	7	1	1	161	4	176	1.33e-101	300

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048035.01	MsG0480019893.01	0.803986	2.682248e-49	1.367099e-46
MsG0480019893.01	MsG0480022479.01	0.815696	8.099802e-52	5.624108e-49
MsG0480019893.01	MsG0480023903.01	0.820921	5.313094e-53	4.260931e-50
MsG0480019893.01	MsG0580024073.01	0.810975	8.821741e-51	5.395007e-48
MsG0480019893.01	MsG0780038113.01	0.818606	1.795204e-52	1.349965e-49
MsG0480019893.01	MsG0780038444.01	0.800745	1.247760e-48	5.855213e-46
MsG0480019893.01	MsG0780038829.01	0.823008	1.745697e-53	1.485021e-50
MsG0480019893.01	MsG0780040679.01	0.800778	1.228642e-48	5.770259e-46
MsG0480019893.01	MsG0880046467.01	0.803640	3.165533e-49	1.599027e-46
MsG0380015931.01	MsG0480019893.01	0.822196	2.696311e-53	2.241157e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019893.01.T01	MTR_0005s0200	87.571	177	10	1	2	166	25	201	5.03e-90	285
MsG0480019893.01.T01	MTR_0003s0170	88.356	146	5	1	1	134	4	149	2.30e-85	252
MsG0480019893.01.T01	MTR_3g070590	56.024	166	72	1	1	166	4	168	1.29e-47	166
MsG0480019893.01.T01	MTR_3g071130	55.422	166	73	1	1	166	4	168	2.31e-47	165
MsG0480019893.01.T01	MTR_3g018930	41.436	181	88	4	3	166	9	188	4.18e-33	124
MsG0480019893.01.T01	MTR_3g014500	38.462	169	95	4	3	164	9	175	4.41e-33	116
MsG0480019893.01.T01	MTR_3g018930	41.436	181	88	4	3	166	9	188	4.77e-33	124
MsG0480019893.01.T01	MTR_3g018980	40.838	191	93	6	3	176	7	194	1.06e-32	123
MsG0480019893.01.T01	MTR_3g018980	40.838	191	93	6	3	176	7	194	1.09e-32	123
MsG0480019893.01.T01	MTR_3g014470	40.113	177	89	5	4	166	8	181	4.81e-31	118
MsG0480019893.01.T01	MTR_3g019040	38.889	180	94	3	3	166	7	186	1.27e-30	117
MsG0480019893.01.T01	MTR_7g021000	39.181	171	90	3	3	165	7	171	1.66e-30	117
MsG0480019893.01.T01	MTR_7g021000	39.181	171	90	3	3	165	7	171	1.73e-30	117
MsG0480019893.01.T01	MTR_3g031940	33.149	181	103	2	3	166	8	187	9.49e-30	114
MsG0480019893.01.T01	MTR_3g033380	32.597	181	104	2	3	166	8	187	1.18e-29	114
MsG0480019893.01.T01	MTR_3g020890	46.108	167	86	4	3	166	8	173	1.69e-29	114
MsG0480019893.01.T01	MTR_3g015260	41.989	181	85	6	3	166	9	186	2.01e-29	114
MsG0480019893.01.T01	MTR_3g015260	41.989	181	85	6	3	166	9	186	2.31e-29	113
MsG0480019893.01.T01	MTR_3g014510	36.517	178	96	3	4	166	8	183	3.41e-29	113
MsG0480019893.01.T01	MTR_3g014390	37.714	175	92	4	6	166	10	181	6.03e-29	112
MsG0480019893.01.T01	MTR_3g014360	38.547	179	90	5	4	166	8	182	1.07e-28	112
MsG0480019893.01.T01	MTR_3g032340	32.418	182	104	2	3	166	8	188	1.97e-28	111
MsG0480019893.01.T01	MTR_2g014730	37.500	168	96	2	4	163	9	175	3.20e-28	110
MsG0480019893.01.T01	MTR_8g030860	32.000	175	106	2	3	165	8	181	5.45e-28	109
MsG0480019893.01.T01	MTR_3g032500	31.492	181	106	2	3	166	8	187	6.89e-28	109
MsG0480019893.01.T01	MTR_3g014080	41.436	181	85	3	4	166	7	184	1.17e-27	108
MsG0480019893.01.T01	MTR_3g014080	41.436	181	85	3	4	166	7	184	1.22e-27	108
MsG0480019893.01.T01	MTR_3g032200	31.492	181	106	2	3	166	8	187	2.05e-27	108
MsG0480019893.01.T01	MTR_3g014320	40.120	167	80	5	16	166	20	182	2.41e-27	108
MsG0480019893.01.T01	MTR_5g095910	39.535	172	92	3	4	163	9	180	2.41e-27	107
MsG0480019893.01.T01	MTR_1g037610	37.725	167	84	5	16	166	20	182	3.68e-27	107
MsG0480019893.01.T01	MTR_8g038570	33.333	192	103	4	3	171	8	197	4.14e-27	107
MsG0480019893.01.T01	MTR_1g047800	39.286	168	96	4	3	166	8	173	5.72e-27	107
MsG0480019893.01.T01	MTR_3g014530	39.516	124	75	0	1	124	2	125	9.88e-27	99.8
MsG0480019893.01.T01	MTR_0267s0070	41.212	165	91	3	4	163	8	171	1.97e-26	105
MsG0480019893.01.T01	MTR_3g032960	34.615	182	95	4	3	166	8	183	9.64e-26	103
MsG0480019893.01.T01	MTR_3g019000	37.143	175	94	3	3	166	7	176	1.35e-25	102
MsG0480019893.01.T01	MTR_3g032800	33.149	181	99	5	3	166	8	183	1.56e-25	102
MsG0480019893.01.T01	MTR_3g033240	34.078	179	98	3	3	165	8	182	2.04e-25	102
MsG0480019893.01.T01	MTR_1g037730	41.420	169	82	4	4	156	9	176	2.26e-25	100
MsG0480019893.01.T01	MTR_2g063777	33.516	182	100	4	1	165	5	182	5.06e-25	101
MsG0480019893.01.T01	MTR_3g014040	41.420	169	82	4	4	156	9	176	5.45e-25	101
MsG0480019893.01.T01	MTR_3g435720	33.146	178	101	4	3	165	8	182	2.05e-24	99.8
MsG0480019893.01.T01	MTR_3g032110	30.000	180	107	3	3	166	8	184	2.26e-24	99.4
MsG0480019893.01.T01	MTR_3g035960	29.775	178	109	2	3	166	8	183	2.84e-24	99.4
MsG0480019893.01.T01	MTR_2g014820	37.278	169	97	2	4	163	9	177	5.72e-24	98.2
MsG0480019893.01.T01	MTR_3g027250	36.842	171	95	3	3	166	7	171	5.81e-24	98.2
MsG0480019893.01.T01	MTR_3g030960	32.222	180	102	3	3	166	8	183	6.58e-24	98.2
MsG0480019893.01.T01	MTR_3g030980	32.222	180	102	3	3	166	8	183	7.49e-24	97.8
MsG0480019893.01.T01	MTR_2g014840	37.126	167	94	4	4	160	9	174	9.30e-24	97.8
MsG0480019893.01.T01	MTR_6g084550	32.402	179	103	3	3	165	8	184	1.05e-23	97.4
MsG0480019893.01.T01	MTR_3g033080	30.814	172	107	3	3	166	8	175	1.24e-23	97.4
MsG0480019893.01.T01	MTR_3g028040	33.520	179	99	4	3	165	8	182	1.29e-23	97.4
MsG0480019893.01.T01	MTR_3g022790	35.795	176	96	3	3	166	7	177	2.58e-23	96.3
MsG0480019893.01.T01	MTR_3g032370	32.222	180	102	3	3	166	6	181	5.96e-23	95.5
MsG0480019893.01.T01	MTR_3g021340	36.364	165	98	2	4	166	9	168	7.74e-23	95.1
MsG0480019893.01.T01	MTR_0425s0020	32.597	181	104	3	3	166	7	186	8.37e-23	95.1
MsG0480019893.01.T01	MTR_4g050410	31.138	167	110	2	3	165	8	173	1.10e-22	94.7
MsG0480019893.01.T01	MTR_8g038590	32.258	186	105	3	3	169	8	191	1.11e-22	94.7
MsG0480019893.01.T01	MTR_2g014830	37.013	154	84	3	4	147	9	159	1.18e-22	89.4
MsG0480019893.01.T01	MTR_3g027420	37.647	170	94	3	3	166	7	170	1.37e-22	94.4
MsG0480019893.01.T01	MTR_3g022300	32.597	181	104	3	3	166	7	186	2.55e-22	93.6
MsG0480019893.01.T01	MTR_3g435740	31.176	170	106	3	3	165	53	218	2.60e-22	93.6
MsG0480019893.01.T01	MTR_3g032150	32.222	180	102	4	3	166	6	181	3.05e-22	93.2
MsG0480019893.01.T01	MTR_0083s0110	30.952	168	101	3	3	157	8	173	3.20e-22	88.6
MsG0480019893.01.T01	MTR_3g027200	31.492	181	106	3	3	166	7	186	3.29e-22	93.2
MsG0480019893.01.T01	MTR_2g039090	36.686	169	98	4	3	169	8	169	4.22e-22	92.8
MsG0480019893.01.T01	MTR_3g021100	33.728	169	100	3	4	166	9	171	4.44e-22	92.8
MsG0480019893.01.T01	MTR_3g033110	31.034	174	103	4	3	166	8	174	9.48e-22	92.0
MsG0480019893.01.T01	MTR_3g022600	31.937	191	110	4	3	176	7	194	1.22e-21	91.7
MsG0480019893.01.T01	MTR_3g035940	30.899	178	107	2	3	166	8	183	1.30e-21	91.7
MsG0480019893.01.T01	MTR_3g021170	34.320	169	95	4	4	166	9	167	1.64e-21	91.3
MsG0480019893.01.T01	MTR_0002s0090	30.899	178	107	2	3	166	8	183	3.38e-21	90.5
MsG0480019893.01.T01	MTR_3g035500	30.899	178	107	2	3	166	8	183	3.38e-21	90.5
MsG0480019893.01.T01	MTR_3g035840	32.768	177	101	4	3	164	8	181	4.78e-21	89.7
MsG0480019893.01.T01	MTR_7g078790	34.706	170	103	3	3	166	6	173	6.45e-21	89.4
MsG0480019893.01.T01	MTR_3g021400	33.742	163	98	2	4	166	2	154	7.09e-21	89.4
MsG0480019893.01.T01	MTR_1g008240	32.738	168	98	5	3	165	4	161	8.06e-21	89.4
MsG0480019893.01.T01	MTR_3g022130	33.918	171	89	4	3	166	7	160	3.60e-20	87.4
MsG0480019893.01.T01	MTR_3g033860	34.078	179	98	4	3	165	8	182	4.05e-20	87.4
MsG0480019893.01.T01	MTR_3g035810	34.503	171	94	4	4	166	9	169	4.56e-20	87.0
MsG0480019893.01.T01	MTR_0002s0110	30.337	178	108	2	3	166	8	183	9.73e-20	86.3
MsG0480019893.01.T01	MTR_3g035480	29.775	178	109	2	3	166	8	183	1.28e-19	85.9
MsG0480019893.01.T01	MTR_3g022230	31.844	179	104	3	3	164	7	184	1.72e-19	85.5
MsG0480019893.01.T01	MTR_3g033130	31.410	156	95	3	3	150	8	159	1.20e-18	83.2
MsG0480019893.01.T01	MTR_3g032760	32.222	180	102	3	3	166	8	183	2.02e-18	82.4
MsG0480019893.01.T01	MTR_5g034770	35.000	180	93	5	8	166	4	180	3.74e-18	81.6
MsG0480019893.01.T01	MTR_3g021380	33.735	166	102	3	3	166	11	170	3.94e-18	81.6
MsG0480019893.01.T01	MTR_3g034430	31.461	178	104	3	3	165	8	182	4.15e-18	81.6
MsG0480019893.01.T01	MTR_5g035280	32.967	182	94	6	8	166	4	180	7.72e-18	80.5
MsG0480019893.01.T01	MTR_5g035300	34.615	182	91	7	8	166	4	180	1.23e-17	79.7
MsG0480019893.01.T01	MTR_5g037450	34.759	187	88	8	8	166	4	184	5.42e-17	78.2
MsG0480019893.01.T01	MTR_3g034380	32.044	181	99	4	3	165	8	182	6.46e-17	78.2
MsG0480019893.01.T01	MTR_5g072140	33.813	139	82	3	36	166	31	167	1.83e-16	76.6
MsG0480019893.01.T01	MTR_3g022400	29.878	164	107	2	3	166	7	162	2.16e-16	76.6
MsG0480019893.01.T01	MTR_5g035240	30.645	186	93	6	8	166	4	180	2.25e-16	76.3
MsG0480019893.01.T01	MTR_3g022400	29.878	164	107	2	3	166	7	162	2.40e-16	76.3
MsG0480019893.01.T01	MTR_3g022940	29.268	164	110	2	4	166	5	163	2.92e-16	76.3
MsG0480019893.01.T01	MTR_5g035480	33.333	186	92	7	8	166	4	184	3.87e-16	75.9
MsG0480019893.01.T01	MTR_3g026660	32.065	184	93	6	8	166	4	180	4.39e-16	75.5
MsG0480019893.01.T01	MTR_3g026660	32.065	184	93	6	8	166	4	180	4.58e-16	75.5
MsG0480019893.01.T01	MTR_5g035230	34.507	142	78	3	39	165	34	175	4.97e-16	75.1
MsG0480019893.01.T01	MTR_5g072340	34.615	130	79	2	43	166	38	167	5.78e-16	75.1
MsG0480019893.01.T01	MTR_3g033160	41.463	82	48	0	1	82	35	116	6.24e-16	70.5
MsG0480019893.01.T01	MTR_5g070960	33.577	137	85	2	36	166	31	167	6.82e-16	75.1
MsG0480019893.01.T01	MTR_1g023600	35.338	133	81	3	38	166	33	164	6.88e-16	75.1
MsG0480019893.01.T01	MTR_5g070490	34.559	136	83	2	37	166	32	167	7.74e-16	74.7
MsG0480019893.01.T01	MTR_0144s0060	32.558	172	98	7	8	166	4	170	8.06e-16	74.7
MsG0480019893.01.T01	MTR_5g071220	35.556	135	81	2	38	166	33	167	8.17e-16	74.7
MsG0480019893.01.T01	MTR_5g071220	35.556	135	81	2	38	166	33	167	8.33e-16	74.7
MsG0480019893.01.T01	MTR_5g071823	34.074	135	83	2	38	166	33	167	1.25e-15	74.3
MsG0480019893.01.T01	MTR_5g071850	34.074	135	83	2	38	166	33	167	1.25e-15	74.3
MsG0480019893.01.T01	MTR_5g071470	35.115	131	77	3	43	166	38	167	1.30e-15	74.3
MsG0480019893.01.T01	MTR_5g070470	33.824	136	84	2	37	166	32	167	1.48e-15	73.9
MsG0480019893.01.T01	MTR_3g023030	30.055	183	108	4	3	166	7	188	2.73e-15	73.2
MsG0480019893.01.T01	MTR_3g023030	30.055	183	108	4	3	166	7	188	2.83e-15	73.2
MsG0480019893.01.T01	MTR_1g045960	30.909	165	98	3	1	163	5	155	2.99e-15	73.2
MsG0480019893.01.T01	MTR_6g046930	32.857	140	88	2	33	166	28	167	3.21e-15	73.2
MsG0480019893.01.T01	MTR_6g046480	35.461	141	82	4	35	166	30	170	3.23e-15	73.2
MsG0480019893.01.T01	MTR_1g022380	33.582	134	82	4	37	166	32	162	3.58e-15	72.8
MsG0480019893.01.T01	MTR_7g071940	33.582	134	82	3	37	166	32	162	5.95e-15	72.4
MsG0480019893.01.T01	MTR_3g007190	44.444	90	50	0	4	93	8	97	6.29e-15	72.4
MsG0480019893.01.T01	MTR_5g071780	33.088	136	85	3	37	166	32	167	9.34e-15	71.6
MsG0480019893.01.T01	MTR_6g046550	35.385	130	75	4	46	166	47	176	1.08e-14	71.6
MsG0480019893.01.T01	MTR_6g052750	34.014	147	79	5	33	166	28	169	1.09e-14	68.9
MsG0480019893.01.T01	MTR_3g025360	26.943	193	107	2	3	166	7	194	1.67e-14	70.9
MsG0480019893.01.T01	MTR_3g435220	31.902	163	90	2	10	163	7	157	6.35e-14	69.3
MsG0480019893.01.T01	MTR_5g072250	33.077	130	81	2	43	166	38	167	7.91e-14	68.9
MsG0480019893.01.T01	MTR_6g047450	34.043	141	84	4	35	166	30	170	8.58e-14	68.9
MsG0480019893.01.T01	MTR_3g022920	30.233	172	91	5	3	166	7	157	9.61e-14	68.9
MsG0480019893.01.T01	MTR_3g086070	32.857	140	85	4	35	166	30	168	1.06e-13	68.6
MsG0480019893.01.T01	MTR_5g071670	32.836	134	83	2	37	166	32	162	1.67e-13	68.2
MsG0480019893.01.T01	MTR_0133s0020	31.765	170	100	7	8	166	4	168	1.99e-13	67.8
MsG0480019893.01.T01	MTR_5g037500	30.769	182	98	6	8	166	4	180	2.54e-13	67.8
MsG0480019893.01.T01	MTR_0133s0030	30.233	172	102	6	8	166	4	170	2.68e-13	67.4
MsG0480019893.01.T01	MTR_6g046570	32.593	135	86	3	37	166	32	166	3.63e-13	67.0
MsG0480019893.01.T01	MTR_6g046130	33.099	142	84	4	35	166	30	170	4.79e-13	66.6
MsG0480019893.01.T01	MTR_5g071800	32.308	130	82	2	43	166	38	167	6.13e-13	66.6
MsG0480019893.01.T01	MTR_3g022870	31.395	172	99	4	3	166	8	168	6.50e-13	66.2
MsG0480019893.01.T01	MTR_3g022870	31.395	172	99	4	3	166	8	168	6.58e-13	66.2
MsG0480019893.01.T01	MTR_0256s0050	31.953	169	101	7	8	166	4	168	1.08e-12	65.9
MsG0480019893.01.T01	MTR_8g036195	33.333	141	84	4	35	166	30	169	1.31e-12	65.5
MsG0480019893.01.T01	MTR_6g046620	34.722	144	79	5	35	166	30	170	1.42e-12	65.5
MsG0480019893.01.T01	MTR_4g055630	33.333	132	78	4	43	166	38	167	1.97e-12	65.1
MsG0480019893.01.T01	MTR_6g046440	35.252	139	83	5	35	166	30	168	1.98e-12	65.1
MsG0480019893.01.T01	MTR_0511s0020	34.028	144	79	6	35	166	30	169	7.15e-12	63.5
MsG0480019893.01.T01	MTR_8g106550	28.859	149	84	3	34	165	9	152	9.90e-12	63.2
MsG0480019893.01.T01	MTR_3g023320	33.333	141	84	4	35	166	30	169	1.04e-11	62.8
MsG0480019893.01.T01	MTR_6g052760	32.877	146	82	6	33	166	28	169	2.55e-11	61.6
MsG0480019893.01.T01	MTR_5g071570	30.469	128	82	2	46	166	41	168	3.56e-11	61.2
MsG0480019893.01.T01	MTR_0230s0010	32.394	142	84	5	35	166	30	169	6.10e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019893.01.T01	AT3G14470	34.637	179	99	2	5	166	8	185	2.48e-27	107
MsG0480019893.01.T01	AT3G14470	34.637	179	99	2	5	166	8	185	2.48e-27	107
MsG0480019893.01.T01	AT3G14460	26.923	182	116	5	1	166	1	181	2.23e-11	62.0

Find 37 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAACGAGTTCAATCTAAAAT+TGG	0.260975	4:+29131832	MsG0480019893.01.T01:CDS
CATCTTGTCTTGTATGGCTT+TGG	0.308541	4:-29131743	None:intergenic
AGGTTCGGAGACAACTTCTT+TGG	0.372060	4:+29131942	MsG0480019893.01.T01:CDS
CAAGACATCATCAGCATCAT+AGG	0.377511	4:-29131707	None:intergenic
AGGATCCATCTTGTCTTGTA+TGG	0.388805	4:-29131749	None:intergenic
CTTGTCTTGTATGGCTTTGG+TGG	0.411532	4:-29131740	None:intergenic
TGCTATCTTGCAAGTACTGT+TGG	0.419307	4:+29131507	MsG0480019893.01.T01:CDS
TTGATCCATTGGTGAAGGAA+TGG	0.449221	4:+29131662	MsG0480019893.01.T01:CDS
GCTGGTGACTGCTATGGAGA+GGG	0.458597	4:+29132026	MsG0480019893.01.T01:CDS
TTCGGAGACAACTTCTTTGG+TGG	0.466078	4:+29131945	MsG0480019893.01.T01:CDS
CTATGATGCTGATGATGTCT+TGG	0.485798	4:+29131708	MsG0480019893.01.T01:CDS
TGATCCATTGGTGAAGGAAT+GGG	0.492850	4:+29131663	MsG0480019893.01.T01:CDS
TTGTTGGCTGGTGACTGCTA+TGG	0.498575	4:+29132020	MsG0480019893.01.T01:CDS
GTAGGTAAACCACTCTCGTT+AGG	0.504865	4:+29131922	MsG0480019893.01.T01:CDS
TCAACCCATTCCTTCACCAA+TGG	0.523915	4:-29131667	None:intergenic
TAAACCACTCTCGTTAGGTT+CGG	0.523915	4:+29131927	MsG0480019893.01.T01:CDS
TCTTGCAAGTACTGTTGGAT+AGG	0.526312	4:+29131512	MsG0480019893.01.T01:CDS
TGGTGACTGCTATGGAGAGG+GGG	0.526494	4:+29132028	MsG0480019893.01.T01:CDS
CTAGAAGTGCCTCATCAAGA+TGG	0.526620	4:-29131573	None:intergenic
GTACGGTAGACATGGTGATA+AGG	0.530924	4:+29131981	MsG0480019893.01.T01:CDS
CTGGTGACTGCTATGGAGAG+GGG	0.532842	4:+29132027	MsG0480019893.01.T01:CDS
GTTCAACACAACCATACATC+AGG	0.532940	4:+29131774	MsG0480019893.01.T01:CDS
AATCTTCTTGGTCTTAAGGA+AGG	0.535624	4:+29131895	MsG0480019893.01.T01:CDS
CAAAGCCATACAAGACAAGA+TGG	0.554442	4:+29131744	MsG0480019893.01.T01:CDS
TACAAGACAAGATGGATCCT+AGG	0.558440	4:+29131752	MsG0480019893.01.T01:CDS
GGTCTTAAGGAAGGAGGTGT+AGG	0.571494	4:+29131904	MsG0480019893.01.T01:CDS
GTGGATGAACATCGTGTGTA+CGG	0.594173	4:+29131964	MsG0480019893.01.T01:CDS
GGCTGGTGACTGCTATGGAG+AGG	0.594317	4:+29132025	MsG0480019893.01.T01:CDS
TACAGTGCTTAATGATGCTG+AGG	0.596768	4:+29131627	MsG0480019893.01.T01:CDS
CTTCTTGGTCTTAAGGAAGG+AGG	0.596908	4:+29131898	MsG0480019893.01.T01:CDS
ACCATCATGGTTGATATGCT+AGG	0.599871	4:+29131472	None:intergenic
GTCTCCGAACCTAACGAGAG+TGG	0.609355	4:-29131931	None:intergenic
GAGTTCAATCTAAAATTGGT+AGG	0.613953	4:+29131836	MsG0480019893.01.T01:CDS
TCGGGGAAACCATCTTGATG+AGG	0.632325	4:+29131564	MsG0480019893.01.T01:CDS
CATCGTGTGTACGGTAGACA+TGG	0.633013	4:+29131973	MsG0480019893.01.T01:CDS
TGTATGGTTGTGTTGAACCT+AGG	0.643182	4:-29131769	None:intergenic
AAATTGGTAGGATAGTTGAG+AGG	0.679446	4:+29131848	MsG0480019893.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTAGAGCATAAAAATCTTCT+TGG	+	Chr4:29131883-29131902	MsG0480019893.01.T01:CDS	25.0%
!!	AGAAGATTATAGACTTTTGT+TGG	+	Chr4:29132004-29132023	MsG0480019893.01.T01:CDS	25.0%
!!	ATAATCTTTTACCTGATGTA+TGG	-	Chr4:29131788-29131807	None:intergenic	25.0%
!!!	TAAAAATCTTCTTGGTCTTA+AGG	+	Chr4:29131891-29131910	MsG0480019893.01.T01:CDS	25.0%
!	GAACGAGTTCAATCTAAAAT+TGG	+	Chr4:29131832-29131851	MsG0480019893.01.T01:CDS	30.0%
!	GAGTTCAATCTAAAATTGGT+AGG	+	Chr4:29131836-29131855	MsG0480019893.01.T01:CDS	30.0%
!!!	GTGATTTTATCGACTTCTTT+CGG	+	Chr4:29131545-29131564	MsG0480019893.01.T01:CDS	30.0%
!!!	TGATTTTATCGACTTCTTTC+GGG	+	Chr4:29131546-29131565	MsG0480019893.01.T01:CDS	30.0%
	AAATTGGTAGGATAGTTGAG+AGG	+	Chr4:29131848-29131867	MsG0480019893.01.T01:CDS	35.0%
	AGATTGAACTCGTTCAGAAA+AGG	-	Chr4:29131827-29131846	None:intergenic	35.0%
	GATTGAACTCGTTCAGAAAA+GGG	-	Chr4:29131826-29131845	None:intergenic	35.0%
!	GATTATAGACTTTTGTTGGC+TGG	+	Chr4:29132008-29132027	MsG0480019893.01.T01:CDS	35.0%
!!	AATCTTCTTGGTCTTAAGGA+AGG	+	Chr4:29131895-29131914	MsG0480019893.01.T01:CDS	35.0%
!!!	GAAGCAGTTTTTTGATCCAT+TGG	+	Chr4:29131651-29131670	MsG0480019893.01.T01:CDS	35.0%
!!!	GATTTTATCGACTTCTTTCG+GGG	+	Chr4:29131547-29131566	MsG0480019893.01.T01:CDS	35.0%
!!!	GTTTTTTGATCCATTGGTGA+AGG	+	Chr4:29131657-29131676	MsG0480019893.01.T01:CDS	35.0%
	AGGATCCATCTTGTCTTGTA+TGG	-	Chr4:29131752-29131771	None:intergenic	40.0%
	CAAAGCCATACAAGACAAGA+TGG	+	Chr4:29131744-29131763	MsG0480019893.01.T01:CDS	40.0%
	CAAGACATCATCAGCATCAT+AGG	-	Chr4:29131710-29131729	None:intergenic	40.0%
	CATCTTGTCTTGTATGGCTT+TGG	-	Chr4:29131746-29131765	None:intergenic	40.0%
	GTTCAACACAACCATACATC+AGG	+	Chr4:29131774-29131793	MsG0480019893.01.T01:CDS	40.0%
	TAAACCACTCTCGTTAGGTT+CGG	+	Chr4:29131927-29131946	MsG0480019893.01.T01:CDS	40.0%
	TACAAGACAAGATGGATCCT+AGG	+	Chr4:29131752-29131771	MsG0480019893.01.T01:CDS	40.0%
	TACAGTGCTTAATGATGCTG+AGG	+	Chr4:29131627-29131646	MsG0480019893.01.T01:CDS	40.0%
	TGATCCATTGGTGAAGGAAT+GGG	+	Chr4:29131663-29131682	MsG0480019893.01.T01:CDS	40.0%
	TGTATGGTTGTGTTGAACCT+AGG	-	Chr4:29131772-29131791	None:intergenic	40.0%
	TTCAGAAAAGGGATTGAGAG+AGG	-	Chr4:29131815-29131834	None:intergenic	40.0%
	TTGATCCATTGGTGAAGGAA+TGG	+	Chr4:29131662-29131681	MsG0480019893.01.T01:CDS	40.0%
!	CTATGATGCTGATGATGTCT+TGG	+	Chr4:29131708-29131727	MsG0480019893.01.T01:CDS	40.0%
!	TCTTGCAAGTACTGTTGGAT+AGG	+	Chr4:29131512-29131531	MsG0480019893.01.T01:CDS	40.0%
!	TGCTATCTTGCAAGTACTGT+TGG	+	Chr4:29131507-29131526	MsG0480019893.01.T01:CDS	40.0%
	AGGTTCGGAGACAACTTCTT+TGG	+	Chr4:29131942-29131961	MsG0480019893.01.T01:CDS	45.0%
	GTACGGTAGACATGGTGATA+AGG	+	Chr4:29131981-29132000	MsG0480019893.01.T01:CDS	45.0%
	GTAGGTAAACCACTCTCGTT+AGG	+	Chr4:29131922-29131941	MsG0480019893.01.T01:CDS	45.0%
	GTGGATGAACATCGTGTGTA+CGG	+	Chr4:29131964-29131983	MsG0480019893.01.T01:CDS	45.0%
	TCAACCCATTCCTTCACCAA+TGG	-	Chr4:29131670-29131689	None:intergenic	45.0%
	TTCGGAGACAACTTCTTTGG+TGG	+	Chr4:29131945-29131964	MsG0480019893.01.T01:CDS	45.0%
!!	CTAGAAGTGCCTCATCAAGA+TGG	-	Chr4:29131576-29131595	None:intergenic	45.0%
!!	CTTCTTGGTCTTAAGGAAGG+AGG	+	Chr4:29131898-29131917	MsG0480019893.01.T01:CDS	45.0%
!!	CTTGTCTTGTATGGCTTTGG+TGG	-	Chr4:29131743-29131762	None:intergenic	45.0%
	CATCGTGTGTACGGTAGACA+TGG	+	Chr4:29131973-29131992	MsG0480019893.01.T01:CDS	50.0%
	TCGGGGAAACCATCTTGATG+AGG	+	Chr4:29131564-29131583	MsG0480019893.01.T01:CDS	50.0%
!	GGTCTTAAGGAAGGAGGTGT+AGG	+	Chr4:29131904-29131923	MsG0480019893.01.T01:CDS	50.0%
!	TTGTTGGCTGGTGACTGCTA+TGG	+	Chr4:29132020-29132039	MsG0480019893.01.T01:CDS	50.0%
	CTGGTGACTGCTATGGAGAG+GGG	+	Chr4:29132027-29132046	MsG0480019893.01.T01:CDS	55.0%
	GCTGGTGACTGCTATGGAGA+GGG	+	Chr4:29132026-29132045	MsG0480019893.01.T01:CDS	55.0%
	GTCTCCGAACCTAACGAGAG+TGG	-	Chr4:29131934-29131953	None:intergenic	55.0%
	TGGTGACTGCTATGGAGAGG+GGG	+	Chr4:29132028-29132047	MsG0480019893.01.T01:CDS	55.0%
	GGCTGGTGACTGCTATGGAG+AGG	+	Chr4:29132025-29132044	MsG0480019893.01.T01:CDS	60.0%
!	CTGCTATGGAGAGGGGGTGC+CGG	+	Chr4:29132034-29132053	MsG0480019893.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	29131487	29132059	29131487	ID=MsG0480019893.01;Name=MsG0480019893.01
Chr4	mRNA	29131487	29132059	29131487	ID=MsG0480019893.01.T01;Parent=MsG0480019893.01;Name=MsG0480019893.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|0\|0\|1\|0\|0\|2\|0\|178
Chr4	exon	29131487	29131795	29131487	ID=MsG0480019893.01.T01:exon:12111;Parent=MsG0480019893.01.T01
Chr4	exon	29131832	29132059	29131832	ID=MsG0480019893.01.T01:exon:12112;Parent=MsG0480019893.01.T01
Chr4	CDS	29131487	29131795	29131487	ID=MsG0480019893.01.T01:cds;Parent=MsG0480019893.01.T01
Chr4	CDS	29131832	29132059	29131832	ID=MsG0480019893.01.T01:cds;Parent=MsG0480019893.01.T01

Gene Sequence

>MsG0480019893.01.T01

ATGCTAGGCTCATTTGTCTCTGCTATCTTGCAAGTACTGTTGGATAGGATAGCTAATCGTGATTTTATCGACTTCTTTCGGGGAAACCATCTTGATGAGGCACTTCTAGACAAGTTGAAGATGCTTTTGCTATCTGTCACTACAGTGCTTAATGATGCTGAGGAGAAGCAGTTTTTTGATCCATTGGTGAAGGAATGGGTTGATAAGCTTAAGAATGCTGCCTATGATGCTGATGATGTCTTGGATGAGATTGCCACCAAAGCCATACAAGACAAGATGGATCCTAGGTTCAACACAACCATACATCAGGAACGAGTTCAATCTAAAATTGGTAGGATAGTTGAGAGGTTGAAATCTATATTAGAGCATAAAAATCTTCTTGGTCTTAAGGAAGGAGGTGTAGGTAAACCACTCTCGTTAGGTTCGGAGACAACTTCTTTGGTGGATGAACATCGTGTGTACGGTAGACATGGTGATAAGGAGAAGATTATAGACTTTTGTTGGCTGGTGACTGCTATGGAGAGGGGGTGCCGGTAG

Protein sequence

>MsG0480019893.01.T01

MLGSFVSAILQVLLDRIANRDFIDFFRGNHLDEALLDKLKMLLLSVTTVLNDAEEKQFFDPLVKEWVDKLKNAAYDADDVLDEIATKAIQDKMDPRFNTTIHQERVQSKIGRIVERLKSILEHKNLLGLKEGGVGKPLSLGSETTSLVDEHRVYGRHGDKEKIIDFCWLVTAMERGCR*