Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015931.01.T01 | XP_003601376.2 | 95.118 | 635 | 17 | 1 | 1 | 635 | 1 | 621 | 0 | 1236 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047825.01 | MsG0380015931.01 | 0.837710 | 4.426198e-57 | 5.798490e-54 |
| MsG0080048035.01 | MsG0380015931.01 | 0.859378 | 4.312324e-63 | 1.136654e-59 |
| MsG0080048177.01 | MsG0380015931.01 | 0.890526 | 9.095674e-74 | 7.674870e-70 |
| MsG0080048178.01 | MsG0380015931.01 | 0.895660 | 7.762231e-76 | 8.129309e-72 |
| MsG0080048204.01 | MsG0380015931.01 | 0.830252 | 3.259860e-55 | 3.416325e-52 |
| MsG0080048205.01 | MsG0380015931.01 | 0.857185 | 1.939011e-62 | 4.745065e-59 |
| MsG0080048207.01 | MsG0380015931.01 | 0.855050 | 8.180538e-62 | 1.864356e-58 |
| MsG0080048210.01 | MsG0380015931.01 | 0.813199 | 2.888605e-51 | 1.874053e-48 |
| MsG0080048309.01 | MsG0380015931.01 | 0.810405 | 1.171640e-50 | 7.056033e-48 |
| MsG0080048602.01 | MsG0380015931.01 | 0.809467 | 1.865392e-50 | 1.096060e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0280008248.01 | MsG0380015931.01 | PPI |
| MsG0380015931.01 | MsG0580029413.01 | PPI |
| MsG0380015931.01 | MsG0580029399.01 | PPI |
| MsG0380015931.01 | MsG0580029400.01 | PPI |
| MsG0580029403.01 | MsG0380015931.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015931.01.T01 | MTR_3g080050 | 95.118 | 635 | 17 | 1 | 1 | 635 | 1 | 621 | 0.0 | 1236 |
| MsG0380015931.01.T01 | MTR_5g086030 | 62.519 | 651 | 214 | 5 | 1 | 635 | 2 | 638 | 0.0 | 836 |
| MsG0380015931.01.T01 | MTR_5g086130 | 59.713 | 628 | 232 | 7 | 13 | 635 | 7 | 618 | 0.0 | 736 |
| MsG0380015931.01.T01 | MTR_5g086130 | 59.365 | 630 | 233 | 7 | 13 | 635 | 7 | 620 | 0.0 | 734 |
| MsG0380015931.01.T01 | MTR_2g024290 | 61.270 | 630 | 213 | 9 | 15 | 635 | 9 | 616 | 0.0 | 725 |
| MsG0380015931.01.T01 | MTR_5g086040 | 56.577 | 631 | 224 | 11 | 9 | 635 | 6 | 590 | 0.0 | 645 |
| MsG0380015931.01.T01 | MTR_5g086540 | 53.821 | 602 | 208 | 7 | 28 | 616 | 21 | 565 | 0.0 | 638 |
| MsG0380015931.01.T01 | MTR_5g086090 | 52.970 | 606 | 241 | 7 | 27 | 616 | 23 | 600 | 0.0 | 634 |
| MsG0380015931.01.T01 | MTR_5g086120 | 51.129 | 620 | 260 | 9 | 13 | 616 | 7 | 599 | 0.0 | 603 |
| MsG0380015931.01.T01 | MTR_2g024290 | 58.379 | 543 | 195 | 9 | 15 | 548 | 9 | 529 | 0.0 | 581 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015931.01.T01 | AT3G21630 | 60.480 | 625 | 219 | 11 | 19 | 635 | 13 | 617 | 0.0 | 724 |
| MsG0380015931.01.T01 | AT1G51940 | 41.287 | 373 | 204 | 8 | 277 | 635 | 280 | 651 | 3.35e-88 | 288 |
| MsG0380015931.01.T01 | AT5G38560 | 37.626 | 396 | 221 | 10 | 242 | 615 | 231 | 622 | 2.78e-72 | 246 |
| MsG0380015931.01.T01 | AT3G24550 | 42.765 | 311 | 167 | 7 | 310 | 615 | 257 | 561 | 2.62e-69 | 238 |
| MsG0380015931.01.T01 | AT1G68690 | 36.951 | 387 | 224 | 11 | 241 | 612 | 273 | 654 | 1.65e-67 | 234 |
| MsG0380015931.01.T01 | AT1G68690 | 36.951 | 387 | 224 | 11 | 241 | 612 | 273 | 654 | 1.65e-67 | 234 |
| MsG0380015931.01.T01 | AT1G68690 | 36.951 | 387 | 224 | 11 | 241 | 612 | 273 | 654 | 1.65e-67 | 234 |
| MsG0380015931.01.T01 | AT1G26150 | 38.903 | 383 | 205 | 12 | 251 | 611 | 332 | 707 | 1.39e-66 | 233 |
| MsG0380015931.01.T01 | AT5G02800 | 42.763 | 304 | 155 | 8 | 324 | 616 | 61 | 356 | 2.20e-66 | 222 |
| MsG0380015931.01.T01 | AT3G24540 | 41.751 | 297 | 165 | 5 | 324 | 615 | 171 | 464 | 5.41e-66 | 224 |
Find 162 sgRNAs with CRISPR-Local
Find 473 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTGGTCAAGGAGGTTT+TGG | 0.253609 | 3:+76803976 | MsG0380015931.01.T01:CDS |
| AAAATTGTGGATCCAAGGCT+TGG | 0.483027 | 3:+76808096 | MsG0380015931.01.T01:CDS |
| AAAGTAGATGTTTATGCCTT+CGG | 0.416128 | 3:+76807843 | MsG0380015931.01.T01:CDS |
| AAAGTCTCACCTGGCCTAAG+CGG | 0.629269 | 3:-76802042 | None:intergenic |
| AAAGTTTGGGAGATGAGAAT+TGG | 0.337957 | 3:-76801376 | None:intergenic |
| AAATACAATCAACCTTTCCC+CGG | 0.589183 | 3:-76806777 | None:intergenic |
| AAATTGTGGATCCAAGGCTT+GGG | 0.444881 | 3:+76808097 | MsG0380015931.01.T01:CDS |
| AACTTGAGTCAGCATTTGCG+TGG | 0.626458 | 3:+76806520 | MsG0380015931.01.T01:CDS |
| AAGGCATAAACATCTACTTT+GGG | 0.479884 | 3:-76807840 | None:intergenic |
| AAGGTGAAGAATAAAGGGAG+AGG | 0.568735 | 3:-76801437 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACAGTGAGAATGACAAT+AGG | - | Chr3:76808208-76808227 | None:intergenic | 30.0% | |
| AAACAAGTGTAGCAAACTTA+GGG | - | Chr3:76807379-76807398 | None:intergenic | 30.0% | |
| AAAGTAGATGTTTATGCCTT+CGG | + | Chr3:76807843-76807862 | MsG0380015931.01.T01:CDS | 30.0% | |
| AACTAGATAAGAGGAAATGA+TGG | + | Chr3:76804534-76804553 | MsG0380015931.01.T01:intron | 30.0% | |
| AAGCTATCTTGTAGATTTCA+TGG | + | Chr3:76807234-76807253 | MsG0380015931.01.T01:intron | 30.0% | |
| AAGGCATAAACATCTACTTT+GGG | - | Chr3:76807843-76807862 | None:intergenic | 30.0% | |
| AAGGGATTTATAGTCATGAA+AGG | + | Chr3:76805583-76805602 | MsG0380015931.01.T01:intron | 30.0% | |
| AATCTTGTGAATCTTATGTC+AGG | + | Chr3:76808372-76808391 | MsG0380015931.01.T01:CDS | 30.0% | |
| AATTGTGACGTGAATTATCA+GGG | - | Chr3:76804279-76804298 | None:intergenic | 30.0% | |
| ACCGTAAAACGTAAAATGAA+AGG | - | Chr3:76804590-76804609 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 76801345 | 76808687 | 76801345 | ID=MsG0380015931.01;Name=MsG0380015931.01 |
| Chr3 | mRNA | 76801345 | 76808687 | 76801345 | ID=MsG0380015931.01.T01;Parent=MsG0380015931.01;Name=MsG0380015931.01.T01;_AED=0.35;_eAED=0.36;_QI=59|0.83|0.84|1|0.75|0.84|13|286|635 |
| Chr3 | exon | 76801345 | 76801566 | 76801345 | ID=MsG0380015931.01.T01:exon:24903;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76801689 | 76802171 | 76801689 | ID=MsG0380015931.01.T01:exon:24904;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76803277 | 76803310 | 76803277 | ID=MsG0380015931.01.T01:exon:24905;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76803383 | 76803429 | 76803383 | ID=MsG0380015931.01.T01:exon:24906;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76803544 | 76803705 | 76803544 | ID=MsG0380015931.01.T01:exon:24907;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76803845 | 76804029 | 76803845 | ID=MsG0380015931.01.T01:exon:24908;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76804851 | 76804940 | 76804851 | ID=MsG0380015931.01.T01:exon:24909;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76806454 | 76806547 | 76806454 | ID=MsG0380015931.01.T01:exon:24910;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76806635 | 76806786 | 76806635 | ID=MsG0380015931.01.T01:exon:24911;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76806867 | 76806961 | 76806867 | ID=MsG0380015931.01.T01:exon:24912;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76807815 | 76807953 | 76807815 | ID=MsG0380015931.01.T01:exon:24913;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76808051 | 76808149 | 76808051 | ID=MsG0380015931.01.T01:exon:24914;Parent=MsG0380015931.01.T01 |
| Chr3 | exon | 76808237 | 76808687 | 76808237 | ID=MsG0380015931.01.T01:exon:24915;Parent=MsG0380015931.01.T01 |
| Chr3 | five_prime_UTR | 76801345 | 76801403 | 76801345 | ID=MsG0380015931.01.T01:five_prime_utr;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76801404 | 76801566 | 76801404 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76801689 | 76802171 | 76801689 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76803277 | 76803310 | 76803277 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76803383 | 76803429 | 76803383 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76803544 | 76803705 | 76803544 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76803845 | 76804029 | 76803845 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76804851 | 76804940 | 76804851 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76806454 | 76806547 | 76806454 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76806635 | 76806786 | 76806635 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76806867 | 76806961 | 76806867 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76807815 | 76807953 | 76807815 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76808051 | 76808149 | 76808051 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | CDS | 76808237 | 76808401 | 76808237 | ID=MsG0380015931.01.T01:cds;Parent=MsG0380015931.01.T01 |
| Chr3 | three_prime_UTR | 76808402 | 76808687 | 76808402 | ID=MsG0380015931.01.T01:three_prime_utr;Parent=MsG0380015931.01.T01 |
| Gene Sequence |
>MsG0380015931.01.T01 ATGGAACATCAACCCAGATTCACCTCCTTCATTCCTCTCCCTTTATTCTTCACCTTACTGGCTTCAATTCCATTCCTAACAGATTCCAAATGCACAAAAGGCTGTCCACTTGCTTTAGCCAACTTTTATGTATCCCAAGGCTCCAACCTCACTTATATCTCAAGTATTATGAGATCGAATATTCAGACAAGACCAGAGGACATAGTTGAATACAGCCGCGAAATAATACCTAGTAAAGACAGTGTTCAAGCTGGTCAAAGACTCAACGTTCCATTCCCATGTGATTGTATTGACGGCGAGTTTCTCGGTCACAAGTTTAGCTACGATGTTCAATCGCGTGACACCTACGAAACTGTTGCTACAAATAATTATGCTAACTTAACAAATGTTGAATGGTTGAGGAGGTTTAATACTTATCCACCGAATGATATTCCTGATACTGGAACGTTGAATGTTACTGTTAATTGTTCTTGTGGGGATAGTGATGTTGGGAATTATGCGTTGTTTGTTACTTATCCGCTTAGGCCAGGTGAGACTTTGGGGTCTGTGGCGAATTCGAGTAAAGTTGATTCGTTGTTGTTGCAGAGGTATAATCCTGGTGTCAATTTTAATCAAGGGAGTGGCATTGTTTTTGTTCCAGGAAAAGATCAGAATGGTAGCTTTGTGTTTTTGAGTTCCAGTTTACTGAAACTCGTGACTGTGTTTTTTATTCTCTTATGCAGTTCAGGTCTTGGTGGTGGGGCTATTGGTGGAATAGCTGTTGGAATAGTGGTTGTTCTTCTGTTAGTGGCAGCTGCTATATATTTTGGATACTTCCGGAAGAAGAAGTTACAAAAGGAGGAACTTTTTTCGCGAGATTCCACTGCACTCTTTTCTCAAGATGGGAAAGATGAAAATTCTCATGGTGCTGCAAACGTAACACAAAGGCCTGGTGTGATGACCGGCATAACAGTCGACAAATCAGTTGAATTCTCATATGATGAACTAGCAGCTGCCTCAGATAACTTTAGTATGGCTAATAAAATTGGTCAAGGAGGTTTTGGCTCTGTCTACTATGCAGAGCTGAGGGGAGAGAAAGCTGCAATCAAGAAGATGGATATGCAAGCAACGAAGGAATTTCTCGCTGAATTGAAAGTCTTGACACGTGTTCATCATCTTAACTTGGTGCGGTTGATTGGATATAGTATTGAGGGCTCTCTTTTCCTTGTCTATGAATACATTGAGAATGGAAACTTGAGTCAGCATTTGCGTGGTTCAGGTAGAGATCCACTGCCATGGGCTACACGTGTGCAAATTGCTTTGGATTCTGCCAGAGGTCTTGAATATATCCATGAGCATACAGTGCCTGTATATATTCATCGTGACATAAAGCCAGCAAATATATTGATAGACAAAAACTTCCGGGGAAAGGTTGCTGATTTTGGATTGACCAAACTGACGGAAGTTGGAAGCTCGTCGCTTCCCACTGGTCGTCTTGTTGGTACATTTGGATACATGCCACCGGAGTATGCTCAATATGGAGACGTTTCTCCCAAAGTAGATGTTTATGCCTTCGGAGTTGTTCTTTATGAACTTATTTCGGCAAAGGAAGCAATTGTCAAGTCAAGCGAATCTGTTGCAGACTCAAAGGGACTTGTTGGTTTGTTTGAAGGAGTTCTTAGTCAGCCTGATCCTACAGAAGATCTTCGTAAAATTGTGGATCCAAGGCTTGGGGATAACTACCCTGTTGATTCAGTTCGCAAGATGGCTCAACTAGCCAAAGCGTGTACACAGGAAAATCCTCAACTGCGTCCGAGTATGAGATCCATTGTAGTTGCTCTTATGACACTTTCTTCAACTACAGACGATTGGGATGTTGGTTCCTTTTACGAAAATCAAAATCTTGTGAATCTTATGTCAGGAAGATAG |
| Protein sequence |
>MsG0380015931.01.T01 MEHQPRFTSFIPLPLFFTLLASIPFLTDSKCTKGCPLALANFYVSQGSNLTYISSIMRSNIQTRPEDIVEYSREIIPSKDSVQAGQRLNVPFPCDCIDGEFLGHKFSYDVQSRDTYETVATNNYANLTNVEWLRRFNTYPPNDIPDTGTLNVTVNCSCGDSDVGNYALFVTYPLRPGETLGSVANSSKVDSLLLQRYNPGVNFNQGSGIVFVPGKDQNGSFVFLSSSLLKLVTVFFILLCSSGLGGGAIGGIAVGIVVVLLLVAAAIYFGYFRKKKLQKEELFSRDSTALFSQDGKDENSHGAANVTQRPGVMTGITVDKSVEFSYDELAAASDNFSMANKIGQGGFGSVYYAELRGEKAAIKKMDMQATKEFLAELKVLTRVHHLNLVRLIGYSIEGSLFLVYEYIENGNLSQHLRGSGRDPLPWATRVQIALDSARGLEYIHEHTVPVYIHRDIKPANILIDKNFRGKVADFGLTKLTEVGSSSLPTGRLVGTFGYMPPEYAQYGDVSPKVDVYAFGVVLYELISAKEAIVKSSESVADSKGLVGLFEGVLSQPDPTEDLRKIVDPRLGDNYPVDSVRKMAQLAKACTQENPQLRPSMRSIVVALMTLSSTTDDWDVGSFYENQNLVNLMSGR* |
