Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020091.01.T01 | AES87914.2 | 84.173 | 139 | 9 | 2 | 1 | 138 | 1 | 127 | 4.58E-64 | 201 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020091.01.T01 | P47925 | 38.739 | 111 | 61 | 4 | 23 | 130 | 23 | 129 | 5.44E-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020091.01.T01 | G7JSH2 | 84.173 | 139 | 9 | 2 | 1 | 138 | 1 | 127 | 2.19e-64 | 201 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048357.01 | MsG0480020091.01 | 0.808479 | 3.034765e-50 | 1.737126e-47 |
| MsG0480020064.01 | MsG0480020091.01 | 0.855160 | 7.601926e-62 | 1.738732e-58 |
| MsG0480020091.01 | MsG0480020096.01 | 0.808325 | 3.273885e-50 | 1.866493e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020091.01.T01 | MTR_4g037480 | 84.173 | 139 | 9 | 2 | 1 | 138 | 1 | 127 | 5.55e-68 | 201 |
| MsG0480020091.01.T01 | MTR_4g037560 | 89.130 | 138 | 15 | 0 | 1 | 138 | 1 | 138 | 1.08e-65 | 196 |
| MsG0480020091.01.T01 | MTR_2g090185 | 53.237 | 139 | 51 | 5 | 1 | 138 | 1 | 126 | 3.82e-30 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020091.01.T01 | AT2G20870 | 38.739 | 111 | 61 | 4 | 23 | 130 | 23 | 129 | 5.55e-13 | 62.4 |
Find 51 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCTAATAATCCATTTAT+TGG | 0.205431 | 4:+33849939 | MsG0480020091.01.T01:CDS |
| TACCCTGGCATTGGACATTT+AGG | 0.235381 | 4:+33849894 | MsG0480020091.01.T01:CDS |
| GTCGTCATTGTCGGAGTTCT+TGG | 0.244678 | 4:-33849823 | None:intergenic |
| CCTCCAGTGCCACCAATAAA+TGG | 0.254443 | 4:-33849951 | None:intergenic |
| CCACCAATAAATGGATTATT+AGG | 0.286771 | 4:-33849942 | None:intergenic |
| CTCTTTCTTGTCGTCATTGT+CGG | 0.295441 | 4:-33849832 | None:intergenic |
| GGAGGATCAGGTTCAGGTTT+AGG | 0.307374 | 4:+33849969 | MsG0480020091.01.T01:CDS |
| CCGCCACCACTGCCAGGATT+TGG | 0.317121 | 4:-33850089 | None:intergenic |
| GGCACTGGAGGATCAGGTTC+AGG | 0.329383 | 4:+33849963 | MsG0480020091.01.T01:CDS |
| TTAGGATTTCCACCGAAATT+CGG | 0.332231 | 4:+33849912 | MsG0480020091.01.T01:CDS |
| TAGGATTTCCACCGAAATTC+GGG | 0.357846 | 4:+33849913 | MsG0480020091.01.T01:CDS |
| GGAACCTCAAAGCCGGGGTT+TGG | 0.358168 | 4:-33850068 | None:intergenic |
| CCTGGCAGTGGTGGCGGCGG+TGG | 0.368789 | 4:+33850095 | MsG0480020091.01.T01:CDS |
| GGCTTTGAGGTTCCAAATCC+TGG | 0.373171 | 4:+33850077 | MsG0480020091.01.T01:CDS |
| ATTGGTGGCACTGGAGGATC+AGG | 0.397280 | 4:+33849957 | MsG0480020091.01.T01:CDS |
| TCAGGTCACAGTTACGTTCC+CGG | 0.409019 | 4:+33850026 | MsG0480020091.01.T01:CDS |
| TCAGGTTTAGGATCAGGATC+AGG | 0.415541 | 4:+33849981 | MsG0480020091.01.T01:CDS |
| TGTTCCAAACCCCGGCTTTG+AGG | 0.417503 | 4:+33850064 | MsG0480020091.01.T01:CDS |
| AATCCATTTATTGGTGGCAC+TGG | 0.434316 | 4:+33849948 | MsG0480020091.01.T01:CDS |
| TCAGGTTCAGGTTTAGGATC+AGG | 0.442027 | 4:+33849975 | MsG0480020091.01.T01:CDS |
| TTAGGAGTAAGCCCGAATTT+CGG | 0.445449 | 4:-33849924 | None:intergenic |
| CCAAATCCTGGCAGTGGTGG+CGG | 0.451387 | 4:+33850089 | MsG0480020091.01.T01:CDS |
| ACAACACGGCAAGCAGTTGC+TGG | 0.458864 | 4:+33849789 | MsG0480020091.01.T01:CDS |
| GGAGCAGGATCAGGATCATC+AGG | 0.476965 | 4:+33850008 | MsG0480020091.01.T01:CDS |
| CCACCGCCGCCACCACTGCC+AGG | 0.483162 | 4:-33850095 | None:intergenic |
| GGATTTGGAACCTCAAAGCC+GGG | 0.491389 | 4:-33850074 | None:intergenic |
| CCTAATAATCCATTTATTGG+TGG | 0.494125 | 4:+33849942 | MsG0480020091.01.T01:CDS |
| AACAAATGTGTCATCGCCAC+CGG | 0.495697 | 4:-33850045 | None:intergenic |
| CCATTTATTGGTGGCACTGG+AGG | 0.501560 | 4:+33849951 | MsG0480020091.01.T01:CDS |
| AATCCTAAATGTCCAATGCC+AGG | 0.501871 | 4:-33849897 | None:intergenic |
| CGGAGTTCTTGGCAATGTTG+CGG | 0.505040 | 4:-33849812 | None:intergenic |
| GAGGTTCCAAATCCTGGCAG+TGG | 0.507650 | 4:+33850083 | MsG0480020091.01.T01:CDS |
| AAACTACATTACCCTGGCAT+TGG | 0.520118 | 4:+33849885 | MsG0480020091.01.T01:CDS |
| TCAGGGATAGGAGCAGGATC+AGG | 0.520901 | 4:+33849999 | MsG0480020091.01.T01:CDS |
| AATCCTGGCAGTGGTGGCGG+CGG | 0.526554 | 4:+33850092 | MsG0480020091.01.T01:CDS |
| CGGTGGTAGAGTTGCATCAA+CGG | 0.529347 | 4:+33850112 | MsG0480020091.01.T01:CDS |
| AGGATTTGGAACCTCAAAGC+CGG | 0.531760 | 4:-33850075 | None:intergenic |
| TCAGGATCAGGGATAGGAGC+AGG | 0.535138 | 4:+33849993 | MsG0480020091.01.T01:CDS |
| GACACATTTGTTCCAAACCC+CGG | 0.566437 | 4:+33850056 | MsG0480020091.01.T01:CDS |
| TTAGGATCAGGATCAGGGAT+AGG | 0.568662 | 4:+33849987 | MsG0480020091.01.T01:CDS |
| CAGGTTTAGGATCAGGATCA+GGG | 0.573753 | 4:+33849982 | MsG0480020091.01.T01:CDS |
| GTTCCAAATCCTGGCAGTGG+TGG | 0.581950 | 4:+33850086 | MsG0480020091.01.T01:CDS |
| GGAGTAAGCCCGAATTTCGG+TGG | 0.586534 | 4:-33849921 | None:intergenic |
| ACAAATGTGTCATCGCCACC+GGG | 0.594099 | 4:-33850044 | None:intergenic |
| CTGCTTGCCGTGTTGTGACA+AGG | 0.611217 | 4:-33849782 | None:intergenic |
| ATCCTAAATGTCCAATGCCA+GGG | 0.617878 | 4:-33849896 | None:intergenic |
| ATATCCTCCTTGTCACAACA+CGG | 0.657664 | 4:+33849775 | MsG0480020091.01.T01:CDS |
| CATGGTAAACTACATTACCC+TGG | 0.662493 | 4:+33849879 | MsG0480020091.01.T01:CDS |
| GATTTGGAACCTCAAAGCCG+GGG | 0.694571 | 4:-33850073 | None:intergenic |
| GGTCACAGTTACGTTCCCGG+TGG | 0.780851 | 4:+33850029 | MsG0480020091.01.T01:CDS |
| CTTGCCGTGTTGTGACAAGG+AGG | 0.782718 | 4:-33849779 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTCCTAATAATCCATTTAT+TGG | + | Chr4:33849939-33849958 | MsG0480020091.01.T01:CDS | 25.0% |
| !! | TTTTTATTCAAGCATGATCA+TGG | + | Chr4:33849861-33849880 | MsG0480020091.01.T01:CDS | 25.0% |
| CCTAATAATCCATTTATTGG+TGG | + | Chr4:33849942-33849961 | MsG0480020091.01.T01:CDS | 30.0% | |
| TCATGCTTGAATAAAAACTG+CGG | - | Chr4:33849858-33849877 | None:intergenic | 30.0% | |
| !! | CCACCAATAAATGGATTATT+AGG | - | Chr4:33849945-33849964 | None:intergenic | 30.0% |
| TTAGGATTTCCACCGAAATT+CGG | + | Chr4:33849912-33849931 | MsG0480020091.01.T01:CDS | 35.0% | |
| AAACTACATTACCCTGGCAT+TGG | + | Chr4:33849885-33849904 | MsG0480020091.01.T01:CDS | 40.0% | |
| AATCCTAAATGTCCAATGCC+AGG | - | Chr4:33849900-33849919 | None:intergenic | 40.0% | |
| ATATCCTCCTTGTCACAACA+CGG | + | Chr4:33849775-33849794 | MsG0480020091.01.T01:CDS | 40.0% | |
| ATCCTAAATGTCCAATGCCA+GGG | - | Chr4:33849899-33849918 | None:intergenic | 40.0% | |
| CATGGTAAACTACATTACCC+TGG | + | Chr4:33849879-33849898 | MsG0480020091.01.T01:CDS | 40.0% | |
| CTCTTTCTTGTCGTCATTGT+CGG | - | Chr4:33849835-33849854 | None:intergenic | 40.0% | |
| TAGGATTTCCACCGAAATTC+GGG | + | Chr4:33849913-33849932 | MsG0480020091.01.T01:CDS | 40.0% | |
| TTAGGAGTAAGCCCGAATTT+CGG | - | Chr4:33849927-33849946 | None:intergenic | 40.0% | |
| ! | AATCCATTTATTGGTGGCAC+TGG | + | Chr4:33849948-33849967 | MsG0480020091.01.T01:CDS | 40.0% |
| AACAAATGTGTCATCGCCAC+CGG | - | Chr4:33850048-33850067 | None:intergenic | 45.0% | |
| GACACATTTGTTCCAAACCC+CGG | + | Chr4:33850056-33850075 | MsG0480020091.01.T01:CDS | 45.0% | |
| TTAGGATCAGGATCAGGGAT+AGG | + | Chr4:33849987-33850006 | MsG0480020091.01.T01:CDS | 45.0% | |
| ! | AGGATTTGGAACCTCAAAGC+CGG | - | Chr4:33850078-33850097 | None:intergenic | 45.0% |
| ! | CAGGTTTAGGATCAGGATCA+GGG | + | Chr4:33849982-33850001 | MsG0480020091.01.T01:CDS | 45.0% |
| ! | TACCCTGGCATTGGACATTT+AGG | + | Chr4:33849894-33849913 | MsG0480020091.01.T01:CDS | 45.0% |
| ! | TCAGGTTCAGGTTTAGGATC+AGG | + | Chr4:33849975-33849994 | MsG0480020091.01.T01:CDS | 45.0% |
| ! | TCAGGTTTAGGATCAGGATC+AGG | + | Chr4:33849981-33850000 | MsG0480020091.01.T01:CDS | 45.0% |
| ACAAATGTGTCATCGCCACC+GGG | - | Chr4:33850047-33850066 | None:intergenic | 50.0% | |
| CCTCCAGTGCCACCAATAAA+TGG | - | Chr4:33849954-33849973 | None:intergenic | 50.0% | |
| CGGAGTTCTTGGCAATGTTG+CGG | - | Chr4:33849815-33849834 | None:intergenic | 50.0% | |
| CGGTGGTAGAGTTGCATCAA+CGG | + | Chr4:33850112-33850131 | MsG0480020091.01.T01:CDS | 50.0% | |
| GTCGTCATTGTCGGAGTTCT+TGG | - | Chr4:33849826-33849845 | None:intergenic | 50.0% | |
| TCAGGTCACAGTTACGTTCC+CGG | + | Chr4:33850026-33850045 | MsG0480020091.01.T01:CDS | 50.0% | |
| ! | CCATTTATTGGTGGCACTGG+AGG | + | Chr4:33849951-33849970 | MsG0480020091.01.T01:CDS | 50.0% |
| ! | GATTTGGAACCTCAAAGCCG+GGG | - | Chr4:33850076-33850095 | None:intergenic | 50.0% |
| ! | GGAGGATCAGGTTCAGGTTT+AGG | + | Chr4:33849969-33849988 | MsG0480020091.01.T01:CDS | 50.0% |
| ! | GGATTTGGAACCTCAAAGCC+GGG | - | Chr4:33850077-33850096 | None:intergenic | 50.0% |
| !! | GGCTTTGAGGTTCCAAATCC+TGG | + | Chr4:33850077-33850096 | MsG0480020091.01.T01:CDS | 50.0% |
| ACAACACGGCAAGCAGTTGC+TGG | + | Chr4:33849789-33849808 | MsG0480020091.01.T01:CDS | 55.0% | |
| CTGCTTGCCGTGTTGTGACA+AGG | - | Chr4:33849785-33849804 | None:intergenic | 55.0% | |
| CTTGCCGTGTTGTGACAAGG+AGG | - | Chr4:33849782-33849801 | None:intergenic | 55.0% | |
| GAGGTTCCAAATCCTGGCAG+TGG | + | Chr4:33850083-33850102 | MsG0480020091.01.T01:CDS | 55.0% | |
| GGAGCAGGATCAGGATCATC+AGG | + | Chr4:33850008-33850027 | MsG0480020091.01.T01:CDS | 55.0% | |
| GGAGTAAGCCCGAATTTCGG+TGG | - | Chr4:33849924-33849943 | None:intergenic | 55.0% | |
| GTTCCAAATCCTGGCAGTGG+TGG | + | Chr4:33850086-33850105 | MsG0480020091.01.T01:CDS | 55.0% | |
| TCAGGATCAGGGATAGGAGC+AGG | + | Chr4:33849993-33850012 | MsG0480020091.01.T01:CDS | 55.0% | |
| TCAGGGATAGGAGCAGGATC+AGG | + | Chr4:33849999-33850018 | MsG0480020091.01.T01:CDS | 55.0% | |
| TGTTCCAAACCCCGGCTTTG+AGG | + | Chr4:33850064-33850083 | MsG0480020091.01.T01:CDS | 55.0% | |
| ! | ATTGGTGGCACTGGAGGATC+AGG | + | Chr4:33849957-33849976 | MsG0480020091.01.T01:CDS | 55.0% |
| CCAAATCCTGGCAGTGGTGG+CGG | + | Chr4:33850089-33850108 | MsG0480020091.01.T01:CDS | 60.0% | |
| GGAACCTCAAAGCCGGGGTT+TGG | - | Chr4:33850071-33850090 | None:intergenic | 60.0% | |
| GGTCACAGTTACGTTCCCGG+TGG | + | Chr4:33850029-33850048 | MsG0480020091.01.T01:CDS | 60.0% | |
| ! | GGCACTGGAGGATCAGGTTC+AGG | + | Chr4:33849963-33849982 | MsG0480020091.01.T01:CDS | 60.0% |
| CCGCCACCACTGCCAGGATT+TGG | - | Chr4:33850092-33850111 | None:intergenic | 65.0% | |
| !! | AATCCTGGCAGTGGTGGCGG+CGG | + | Chr4:33850092-33850111 | MsG0480020091.01.T01:CDS | 65.0% |
| ! | CCACCGCCGCCACCACTGCC+AGG | - | Chr4:33850098-33850117 | None:intergenic | 80.0% |
| !!! | CCTGGCAGTGGTGGCGGCGG+TGG | + | Chr4:33850095-33850114 | MsG0480020091.01.T01:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 33849729 | 33850145 | 33849729 | ID=MsG0480020091.01;Name=MsG0480020091.01 |
| Chr4 | mRNA | 33849729 | 33850145 | 33849729 | ID=MsG0480020091.01.T01;Parent=MsG0480020091.01;Name=MsG0480020091.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|138 |
| Chr4 | exon | 33849729 | 33850145 | 33849729 | ID=MsG0480020091.01.T01:exon:55251;Parent=MsG0480020091.01.T01 |
| Chr4 | CDS | 33849729 | 33850145 | 33849729 | ID=MsG0480020091.01.T01:cds;Parent=MsG0480020091.01.T01 |
| Gene Sequence |
| Protein sequence |
