Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020096.01.T01 | XP_003605725.1 | 90.541 | 74 | 7 | 0 | 1 | 74 | 1 | 74 | 1.54E-41 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020096.01.T01 | G7JSI0 | 90.541 | 74 | 7 | 0 | 1 | 74 | 1 | 74 | 7.33e-42 | 145 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048357.01 | MsG0480020096.01 | 0.860097 | 2.619169e-63 | 7.077095e-60 |
| MsG0180000632.01 | MsG0480020096.01 | 0.810576 | 1.076217e-50 | 6.511173e-48 |
| MsG0180001771.01 | MsG0480020096.01 | 0.808905 | 2.461411e-50 | 1.424645e-47 |
| MsG0180003741.01 | MsG0480020096.01 | 0.831185 | 1.926495e-55 | 2.074875e-52 |
| MsG0380011496.01 | MsG0480020096.01 | 0.849832 | 2.510151e-60 | 4.820018e-57 |
| MsG0380011501.01 | MsG0480020096.01 | 0.829598 | 4.705661e-55 | 4.838808e-52 |
| MsG0480018909.01 | MsG0480020096.01 | 0.840446 | 8.652015e-58 | 1.232760e-54 |
| MsG0480020064.01 | MsG0480020096.01 | 0.805954 | 1.040332e-49 | 5.577439e-47 |
| MsG0480020091.01 | MsG0480020096.01 | 0.808325 | 3.273885e-50 | 1.866493e-47 |
| MsG0480020096.01 | MsG0480024022.01 | 0.838361 | 3.009573e-57 | 4.021555e-54 |
| MsG0480020096.01 | MsG0580025466.01 | 0.803612 | 3.208199e-49 | 1.619434e-46 |
| MsG0480020096.01 | MsG0580025973.01 | 0.806903 | 6.561053e-50 | 3.603920e-47 |
| MsG0480020096.01 | MsG0580027925.01 | 0.832009 | 1.207011e-55 | 1.332180e-52 |
| MsG0480020096.01 | MsG0580029310.01 | 0.820616 | 6.242274e-53 | 4.963315e-50 |
| MsG0480020096.01 | MsG0580029418.01 | 0.844560 | 7.011096e-59 | 1.136327e-55 |
| MsG0480020096.01 | MsG0680030328.01 | 0.808043 | 3.758819e-50 | 2.126919e-47 |
| MsG0480020096.01 | MsG0680031707.01 | 0.831048 | 2.081325e-55 | 2.232611e-52 |
| MsG0480020096.01 | MsG0680031959.01 | 0.817767 | 2.780331e-52 | 2.043179e-49 |
| MsG0480020096.01 | MsG0780040846.01 | 0.854333 | 1.320036e-61 | 2.937796e-58 |
| MsG0480020096.01 | MsG0780040966.01 | 0.824238 | 8.992968e-54 | 7.920129e-51 |
| MsG0480020096.01 | MsG0780041259.01 | 0.813273 | 2.781958e-51 | 1.808565e-48 |
| MsG0480020096.01 | MsG0780041698.01 | 0.841756 | 3.917444e-58 | 5.813193e-55 |
| MsG0480020096.01 | MsG0880041858.01 | 0.839713 | 1.344252e-57 | 1.872030e-54 |
| MsG0480020096.01 | MsG0880042959.01 | 0.801364 | 9.324791e-49 | 4.444990e-46 |
| MsG0480020096.01 | MsG0880045585.01 | 0.801684 | 8.017204e-49 | 3.852456e-46 |
| MsG0480020096.01 | MsG0880045586.01 | 0.814095 | 1.834211e-51 | 1.219319e-48 |
| MsG0280007305.01 | MsG0480020096.01 | 0.828048 | 1.116183e-54 | 1.096942e-51 |
| MsG0280008390.01 | MsG0480020096.01 | 0.802764 | 4.806117e-49 | 2.373984e-46 |
| MsG0280008391.01 | MsG0480020096.01 | 0.823581 | 1.282589e-53 | 1.108828e-50 |
| MsG0280008392.01 | MsG0480020096.01 | 0.821315 | 4.309688e-53 | 3.494187e-50 |
| MsG0280008395.01 | MsG0480020096.01 | 0.843850 | 1.086974e-58 | 1.722971e-55 |
| MsG0280010320.01 | MsG0480020096.01 | 0.810346 | 1.206733e-50 | 7.256133e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020096.01.T01 | MTR_4g037560 | 90.541 | 74 | 7 | 0 | 1 | 74 | 1 | 74 | 1.86e-45 | 145 |
| MsG0480020096.01.T01 | MTR_4g037480 | 82.432 | 74 | 13 | 0 | 1 | 74 | 1 | 74 | 2.00e-41 | 134 |
| MsG0480020096.01.T01 | MTR_2g090185 | 48.611 | 72 | 31 | 2 | 1 | 72 | 1 | 66 | 3.82e-13 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCCAGACATTGGGCATTT+AGG | 0.135171 | 4:+33999023 | MsG0480020096.01.T01:CDS |
| ATCCAGACATTGGGCATTTA+GGG | 0.239103 | 4:+33999024 | MsG0480020096.01.T01:CDS |
| CTCTTTCTTGTCGTCGTTGT+TGG | 0.251145 | 4:-33998961 | None:intergenic |
| GGCTTTGAGGTTCCGAATCC+TGG | 0.323999 | 4:+33999193 | MsG0480020096.01.T01:CDS |
| TCAGGATCAGGTTCGAGTTC+AGG | 0.362175 | 4:+33999115 | MsG0480020096.01.T01:CDS |
| TGTTCCAAACCCTGGCTTTG+AGG | 0.364651 | 4:+33999180 | MsG0480020096.01.T01:CDS |
| CCACCGACACCACCACTTCC+AGG | 0.370147 | 4:-33999211 | None:intergenic |
| GGAACCTCAAAGCCAGGGTT+TGG | 0.387167 | 4:-33999184 | None:intergenic |
| TACTCCTAATACCCATTCAT+TGG | 0.394942 | 4:+33999067 | MsG0480020096.01.T01:CDS |
| GCCACCAATGAATGGGTATT+AGG | 0.395452 | 4:-33999071 | None:intergenic |
| AACCCTAAATGCCCAATGTC+TGG | 0.399885 | 4:-33999026 | None:intergenic |
| TACCCATTCATTGGTGGCAC+TGG | 0.410806 | 4:+33999076 | MsG0480020096.01.T01:CDS |
| TCAGGATCGGGCTCAGGATC+AGG | 0.414548 | 4:+33999103 | MsG0480020096.01.T01:CDS |
| TCGGGTCACAGTTATGTTCC+TGG | 0.415011 | 4:+33999142 | MsG0480020096.01.T01:CDS |
| ATTGGTGGCACTGGAGGATC+AGG | 0.420721 | 4:+33999085 | MsG0480020096.01.T01:CDS |
| TTCATGGATGAGTGAATTCA+TGG | 0.435973 | 4:-33999256 | None:intergenic |
| CCTCCAGTGCCACCAATGAA+TGG | 0.439004 | 4:-33999079 | None:intergenic |
| GGCACTGGAGGATCAGGATC+GGG | 0.449576 | 4:+33999091 | MsG0480020096.01.T01:CDS |
| AACTACATTATCCAGACATT+GGG | 0.479429 | 4:+33999015 | MsG0480020096.01.T01:CDS |
| ACACCACCACTTCCAGGATT+CGG | 0.485620 | 4:-33999205 | None:intergenic |
| TGGCACTGGAGGATCAGGAT+CGG | 0.491247 | 4:+33999090 | MsG0480020096.01.T01:CDS |
| CTCCAGTGCCACCAATGAAT+GGG | 0.491401 | 4:-33999078 | None:intergenic |
| GGAGGATCAGGATCGGGCTC+AGG | 0.506667 | 4:+33999097 | MsG0480020096.01.T01:CDS |
| TTTCTGTTCAAGCACGAACA+TGG | 0.515179 | 4:+33998990 | MsG0480020096.01.T01:CDS |
| GGTTCGAGTTCAGGATCATC+GGG | 0.521084 | 4:+33999124 | MsG0480020096.01.T01:CDS |
| GGATTCGGAACCTCAAAGCC+AGG | 0.521240 | 4:-33999190 | None:intergenic |
| TCCTAATACCCATTCATTGG+TGG | 0.522504 | 4:+33999070 | MsG0480020096.01.T01:CDS |
| AAACTACATTATCCAGACAT+TGG | 0.527033 | 4:+33999014 | MsG0480020096.01.T01:CDS |
| AGGTTCGAGTTCAGGATCAT+CGG | 0.528998 | 4:+33999123 | MsG0480020096.01.T01:CDS |
| CCATTCATTGGTGGCACTGG+AGG | 0.530848 | 4:+33999079 | MsG0480020096.01.T01:CDS |
| GAGGTTCCGAATCCTGGAAG+TGG | 0.538023 | 4:+33999199 | MsG0480020096.01.T01:CDS |
| ACAACACAAGAAGCAATTGC+TGG | 0.546670 | 4:+33998918 | MsG0480020096.01.T01:CDS |
| TTCTGTTCAAGCACGAACAT+GGG | 0.565643 | 4:+33998991 | MsG0480020096.01.T01:CDS |
| GACACATTTGTTCCAAACCC+TGG | 0.566936 | 4:+33999172 | MsG0480020096.01.T01:CDS |
| TTGCTTCTTGTGTTGTGACA+AGG | 0.572188 | 4:-33998911 | None:intergenic |
| ACAAATGTGTCATCGCCACC+AGG | 0.593265 | 4:-33999160 | None:intergenic |
| GAGTAAGTCCAATGTGTGGT+GGG | 0.596087 | 4:-33999049 | None:intergenic |
| GGAGTAAGTCCAATGTGTGG+TGG | 0.604268 | 4:-33999050 | None:intergenic |
| CCTGGAAGTGGTGGTGTCGG+TGG | 0.607001 | 4:+33999211 | MsG0480020096.01.T01:CDS |
| AATCCTGGAAGTGGTGGTGT+CGG | 0.618339 | 4:+33999208 | MsG0480020096.01.T01:CDS |
| GATTCGGAACCTCAAAGCCA+GGG | 0.619017 | 4:-33999189 | None:intergenic |
| TTAGGGTTCCCACCACACAT+TGG | 0.624367 | 4:+33999041 | MsG0480020096.01.T01:CDS |
| GTTCCGAATCCTGGAAGTGG+TGG | 0.658317 | 4:+33999202 | MsG0480020096.01.T01:CDS |
| CTTCTTGTGTTGTGACAAGG+AGG | 0.682920 | 4:-33998908 | None:intergenic |
| TTAGGAGTAAGTCCAATGTG+TGG | 0.685070 | 4:-33999053 | None:intergenic |
| TCGTGCTTGAACAGAAACTG+AGG | 0.705299 | 4:-33998984 | None:intergenic |
| GGTCACAGTTATGTTCCTGG+TGG | 0.707662 | 4:+33999145 | MsG0480020096.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACTACATTATCCAGACAT+TGG | + | Chr4:33999014-33999033 | MsG0480020096.01.T01:CDS | 30.0% | |
| AACTACATTATCCAGACATT+GGG | + | Chr4:33999015-33999034 | MsG0480020096.01.T01:CDS | 30.0% | |
| TACTCCTAATACCCATTCAT+TGG | + | Chr4:33999067-33999086 | MsG0480020096.01.T01:CDS | 35.0% | |
| ACAACACAAGAAGCAATTGC+TGG | + | Chr4:33998918-33998937 | MsG0480020096.01.T01:CDS | 40.0% | |
| ATCCAGACATTGGGCATTTA+GGG | + | Chr4:33999024-33999043 | MsG0480020096.01.T01:CDS | 40.0% | |
| TATCCAGACATTGGGCATTT+AGG | + | Chr4:33999023-33999042 | MsG0480020096.01.T01:CDS | 40.0% | |
| TCCTAATACCCATTCATTGG+TGG | + | Chr4:33999070-33999089 | MsG0480020096.01.T01:CDS | 40.0% | |
| TTAGGAGTAAGTCCAATGTG+TGG | - | Chr4:33999056-33999075 | None:intergenic | 40.0% | |
| TTCTGTTCAAGCACGAACAT+GGG | + | Chr4:33998991-33999010 | MsG0480020096.01.T01:CDS | 40.0% | |
| TTGCTTCTTGTGTTGTGACA+AGG | - | Chr4:33998914-33998933 | None:intergenic | 40.0% | |
| TTTCTGTTCAAGCACGAACA+TGG | + | Chr4:33998990-33999009 | MsG0480020096.01.T01:CDS | 40.0% | |
| AACCCTAAATGCCCAATGTC+TGG | - | Chr4:33999029-33999048 | None:intergenic | 45.0% | |
| CTCTTTCTTGTCGTCGTTGT+TGG | - | Chr4:33998964-33998983 | None:intergenic | 45.0% | |
| CTTCTTGTGTTGTGACAAGG+AGG | - | Chr4:33998911-33998930 | None:intergenic | 45.0% | |
| GACACATTTGTTCCAAACCC+TGG | + | Chr4:33999172-33999191 | MsG0480020096.01.T01:CDS | 45.0% | |
| GAGTAAGTCCAATGTGTGGT+GGG | - | Chr4:33999052-33999071 | None:intergenic | 45.0% | |
| GCCACCAATGAATGGGTATT+AGG | - | Chr4:33999074-33999093 | None:intergenic | 45.0% | |
| TCGTGCTTGAACAGAAACTG+AGG | - | Chr4:33998987-33999006 | None:intergenic | 45.0% | |
| ! | AGGTTCGAGTTCAGGATCAT+CGG | + | Chr4:33999123-33999142 | MsG0480020096.01.T01:CDS | 45.0% |
| ACAAATGTGTCATCGCCACC+AGG | - | Chr4:33999163-33999182 | None:intergenic | 50.0% | |
| ACACCACCACTTCCAGGATT+CGG | - | Chr4:33999208-33999227 | None:intergenic | 50.0% | |
| CTCCAGTGCCACCAATGAAT+GGG | - | Chr4:33999081-33999100 | None:intergenic | 50.0% | |
| GATTCGGAACCTCAAAGCCA+GGG | - | Chr4:33999192-33999211 | None:intergenic | 50.0% | |
| GGAGTAAGTCCAATGTGTGG+TGG | - | Chr4:33999053-33999072 | None:intergenic | 50.0% | |
| GGTCACAGTTATGTTCCTGG+TGG | + | Chr4:33999145-33999164 | MsG0480020096.01.T01:CDS | 50.0% | |
| TCGGGTCACAGTTATGTTCC+TGG | + | Chr4:33999142-33999161 | MsG0480020096.01.T01:CDS | 50.0% | |
| TTAGGGTTCCCACCACACAT+TGG | + | Chr4:33999041-33999060 | MsG0480020096.01.T01:CDS | 50.0% | |
| ! | AATCCTGGAAGTGGTGGTGT+CGG | + | Chr4:33999208-33999227 | MsG0480020096.01.T01:CDS | 50.0% |
| ! | GGTTCGAGTTCAGGATCATC+GGG | + | Chr4:33999124-33999143 | MsG0480020096.01.T01:CDS | 50.0% |
| ! | TACCCATTCATTGGTGGCAC+TGG | + | Chr4:33999076-33999095 | MsG0480020096.01.T01:CDS | 50.0% |
| ! | TCAGGATCAGGTTCGAGTTC+AGG | + | Chr4:33999115-33999134 | MsG0480020096.01.T01:CDS | 50.0% |
| !! | TGTTCCAAACCCTGGCTTTG+AGG | + | Chr4:33999180-33999199 | MsG0480020096.01.T01:CDS | 50.0% |
| CCTCCAGTGCCACCAATGAA+TGG | - | Chr4:33999082-33999101 | None:intergenic | 55.0% | |
| GAGGTTCCGAATCCTGGAAG+TGG | + | Chr4:33999199-33999218 | MsG0480020096.01.T01:CDS | 55.0% | |
| GGAACCTCAAAGCCAGGGTT+TGG | - | Chr4:33999187-33999206 | None:intergenic | 55.0% | |
| GGATTCGGAACCTCAAAGCC+AGG | - | Chr4:33999193-33999212 | None:intergenic | 55.0% | |
| GTTCCGAATCCTGGAAGTGG+TGG | + | Chr4:33999202-33999221 | MsG0480020096.01.T01:CDS | 55.0% | |
| ! | ATTGGTGGCACTGGAGGATC+AGG | + | Chr4:33999085-33999104 | MsG0480020096.01.T01:CDS | 55.0% |
| ! | CCATTCATTGGTGGCACTGG+AGG | + | Chr4:33999079-33999098 | MsG0480020096.01.T01:CDS | 55.0% |
| ! | TGGCACTGGAGGATCAGGAT+CGG | + | Chr4:33999090-33999109 | MsG0480020096.01.T01:CDS | 55.0% |
| !! | GGCTTTGAGGTTCCGAATCC+TGG | + | Chr4:33999193-33999212 | MsG0480020096.01.T01:CDS | 55.0% |
| TCAGGATCGGGCTCAGGATC+AGG | + | Chr4:33999103-33999122 | MsG0480020096.01.T01:CDS | 60.0% | |
| ! | GGCACTGGAGGATCAGGATC+GGG | + | Chr4:33999091-33999110 | MsG0480020096.01.T01:CDS | 60.0% |
| CCACCGACACCACCACTTCC+AGG | - | Chr4:33999214-33999233 | None:intergenic | 65.0% | |
| GGAGGATCAGGATCGGGCTC+AGG | + | Chr4:33999097-33999116 | MsG0480020096.01.T01:CDS | 65.0% | |
| ! | CCTGGAAGTGGTGGTGTCGG+TGG | + | Chr4:33999211-33999230 | MsG0480020096.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 33998858 | 33999277 | 33998858 | ID=MsG0480020096.01;Name=MsG0480020096.01 |
| Chr4 | mRNA | 33998858 | 33999277 | 33998858 | ID=MsG0480020096.01.T01;Parent=MsG0480020096.01;Name=MsG0480020096.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|139 |
| Chr4 | exon | 33998858 | 33999277 | 33998858 | ID=MsG0480020096.01.T01:exon:55191;Parent=MsG0480020096.01.T01 |
| Chr4 | CDS | 33998858 | 33999277 | 33998858 | ID=MsG0480020096.01.T01:cds;Parent=MsG0480020096.01.T01 |
| Gene Sequence |
| Protein sequence |
