AlfalfaGEDB | MsG0480020064.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020064.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020064.01.T01	AES87914.2	82.014	139	13	2	1	139	1	127	1.28E-60	192

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020064.01.T01	G7JSH2	82.014	139	13	2	1	139	1	127	6.10e-61	192

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048357.01	MsG0480020064.01	0.823894	1.082988e-53	9.446366e-51
MsG0180003741.01	MsG0480020064.01	0.833455	5.280911e-56	6.084796e-53
MsG0180004389.01	MsG0480020064.01	0.817214	3.704427e-52	2.681224e-49
MsG0180004975.01	MsG0480020064.01	0.811724	6.066568e-51	3.783768e-48
MsG0280011014.01	MsG0480020064.01	0.804315	2.290957e-49	1.177489e-46
MsG0380011496.01	MsG0480020064.01	0.839414	1.607037e-57	2.217776e-54
MsG0380011501.01	MsG0480020064.01	0.836960	6.886397e-57	8.816036e-54
MsG0480018909.01	MsG0480020064.01	0.814852	1.247415e-51	8.463868e-49
MsG0480019922.01	MsG0480020064.01	0.809079	2.259027e-50	1.313693e-47
MsG0480020064.01	MsG0480020091.01	0.855160	7.601926e-62	1.738732e-58
MsG0480020064.01	MsG0480020096.01	0.805954	1.040332e-49	5.577439e-47
MsG0480020064.01	MsG0480020495.01	0.802718	4.912617e-49	2.423721e-46
MsG0480020064.01	MsG0580027925.01	0.840311	9.387019e-58	1.331743e-54
MsG0480020064.01	MsG0580028436.01	0.801134	1.039057e-48	4.924490e-46
MsG0480020064.01	MsG0680030328.01	0.819359	1.210835e-52	9.296451e-50
MsG0480020064.01	MsG0680031959.01	0.803417	3.520352e-49	1.768128e-46
MsG0480020064.01	MsG0780036815.01	0.801268	9.754901e-49	4.638711e-46
MsG0480020064.01	MsG0780040846.01	0.829291	5.587620e-55	5.695353e-52
MsG0480020064.01	MsG0780040966.01	0.804630	1.969605e-49	1.020549e-46
MsG0480020064.01	MsG0780041259.01	0.807559	4.763842e-50	2.661669e-47
MsG0480020064.01	MsG0780041698.01	0.808440	3.094717e-50	1.769629e-47
MsG0480020064.01	MsG0880041858.01	0.803852	2.860401e-49	1.452832e-46
MsG0480020064.01	MsG0880044782.01	0.829502	4.966182e-55	5.092043e-52
MsG0480020064.01	MsG0880045453.01	0.812322	4.493821e-51	2.848430e-48
MsG0480020064.01	MsG0880045585.01	0.801736	7.820609e-49	3.762958e-46
MsG0480020064.01	MsG0880045586.01	0.805429	1.340441e-49	7.090078e-47
MsG0380015549.01	MsG0480020064.01	0.815790	7.716440e-52	5.372070e-49
MsG0380016920.01	MsG0480020064.01	0.801181	1.016338e-48	4.822346e-46
MsG0380016970.01	MsG0480020064.01	0.805221	1.482100e-49	7.797300e-47
MsG0280008395.01	MsG0480020064.01	0.805302	1.425453e-49	7.515001e-47
MsG0280009794.01	MsG0480020064.01	0.806758	7.040409e-50	3.852719e-47
MsG0280010489.01	MsG0480020064.01	0.827251	1.734516e-54	1.665475e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020064.01.T01	MTR_4g037480	82.014	139	13	2	1	139	1	127	1.55e-64	192
MsG0480020064.01.T01	MTR_4g037560	82.270	141	20	3	1	139	1	138	1.59e-59	180
MsG0480020064.01.T01	MTR_2g090185	51.079	139	55	5	1	139	1	126	3.35e-27	98.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020064.01.T01	AT2G20870	41.414	99	53	4	35	130	33	129	1.03e-11	58.9

Find 47 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCCTAATAATCCATTTAT+TGG	0.205431	4:-33177105	MsG0480020064.01.T01:CDS
TACCCTGGCATTGGACATTT+AGG	0.235381	4:-33177150	MsG0480020064.01.T01:CDS
CCTCCAGTGCCACCAATAAA+TGG	0.254443	4:+33177093	None:intergenic
GGAGGATCAGGTTCGGGTTT+AGG	0.265984	4:-33177075	MsG0480020064.01.T01:CDS
CCACCACCATTGCCGGGATT+TGG	0.266687	4:+33176961	None:intergenic
CCACCAATAAATGGATTATT+AGG	0.286771	4:+33177102	None:intergenic
GGCACTGGAGGATCAGGTTC+GGG	0.362114	4:-33177081	MsG0480020064.01.T01:CDS
TGTTCCAAACCCTGGCTTTG+AGG	0.364651	4:-33176986	MsG0480020064.01.T01:CDS
GGAACCTCAAAGCCAGGGTT+TGG	0.367827	4:+33176982	None:intergenic
TTAGGATTTCCACCGAAAAT+CGG	0.378656	4:-33177132	MsG0480020064.01.T01:CDS
TGGCACTGGAGGATCAGGTT+CGG	0.387534	4:-33177082	MsG0480020064.01.T01:CDS
CCCGGCAATGGTGGTGGCGG+CGG	0.389210	4:-33176955	MsG0480020064.01.T01:CDS
ATTGGTGGCACTGGAGGATC+AGG	0.397280	4:-33177087	MsG0480020064.01.T01:CDS
GGCAATGGTGGTGGCGGCGG+TGG	0.404461	4:-33176952	MsG0480020064.01.T01:CDS
CTCTTTCTTGTCGTCGCTGT+CGG	0.412216	4:+33177212	None:intergenic
GGCTTTGAGGTTCCAAATCC+CGG	0.413720	4:-33176973	MsG0480020064.01.T01:CDS
ACCGCCGCCACCACCATTGC+CGG	0.428039	4:+33176954	None:intergenic
TCAGGTTCGGGTTTAGGATC+AGG	0.431514	4:-33177069	MsG0480020064.01.T01:CDS
AATCCATTTATTGGTGGCAC+TGG	0.434316	4:-33177096	MsG0480020064.01.T01:CDS
TCAGGTCATAGTTATGTTCC+CGG	0.442709	4:-33177024	MsG0480020064.01.T01:CDS
TAGGATTTCCACCGAAAATC+GGG	0.446688	4:-33177131	MsG0480020064.01.T01:CDS
CCAAATCCCGGCAATGGTGG+TGG	0.459772	4:-33176961	MsG0480020064.01.T01:CDS
TCAGGAACAGGAGCAGGATC+AGG	0.471562	4:-33177051	MsG0480020064.01.T01:CDS
GGAGCAGGATCAGGATCATC+AGG	0.472228	4:-33177042	MsG0480020064.01.T01:CDS
GGATTTGGAACCTCAAAGCC+AGG	0.493115	4:+33176976	None:intergenic
CCTAATAATCCATTTATTGG+TGG	0.494125	4:-33177102	MsG0480020064.01.T01:CDS
ACATCACAGCAAGCAGTTGC+TGG	0.494474	4:-33177261	MsG0480020064.01.T01:CDS
AACAAATGTGTCATCGCCAC+CGG	0.495697	4:+33177005	None:intergenic
CCATTTATTGGTGGCACTGG+AGG	0.501560	4:-33177093	MsG0480020064.01.T01:CDS
AATCCTAAATGTCCAATGCC+AGG	0.501871	4:+33177147	None:intergenic
TCGGGTTTAGGATCAGGAAC+AGG	0.510472	4:-33177063	MsG0480020064.01.T01:CDS
AAACTACATTACCCTGGCAT+TGG	0.520118	4:-33177159	MsG0480020064.01.T01:CDS
AATCCCGGCAATGGTGGTGG+CGG	0.520657	4:-33176958	MsG0480020064.01.T01:CDS
CCGCCGCCACCACCATTGCC+GGG	0.521155	4:+33176955	None:intergenic
CGGTGGTAGAGTTGCATCAA+CGG	0.529347	4:-33176935	MsG0480020064.01.T01:CDS
GAGGTTCCAAATCCCGGCAA+TGG	0.537778	4:-33176967	MsG0480020064.01.T01:CDS
TTAGGATCAGGAACAGGAGC+AGG	0.546096	4:-33177057	MsG0480020064.01.T01:CDS
GACACATTTGTTCCAAACCC+TGG	0.566936	4:-33176994	MsG0480020064.01.T01:CDS
ACAAATGTGTCATCGCCACC+GGG	0.594099	4:+33177006	None:intergenic
CTGCTTGCTGTGATGTGACA+AGG	0.599798	4:+33177268	None:intergenic
GTTCCAAATCCCGGCAATGG+TGG	0.615486	4:-33176964	MsG0480020064.01.T01:CDS
ATCCTAAATGTCCAATGCCA+GGG	0.617878	4:+33177148	None:intergenic
TCTTAGCATTAGCAATGCTG+CGG	0.631738	4:+33177238	None:intergenic
GATTTGGAACCTCAAAGCCA+GGG	0.635813	4:+33176977	None:intergenic
CATGGTAAACTACATTACCC+TGG	0.662493	4:-33177165	MsG0480020064.01.T01:CDS
GGTCATAGTTATGTTCCCGG+TGG	0.737137	4:-33177021	MsG0480020064.01.T01:CDS
CTTGCTGTGATGTGACAAGG+AGG	0.757626	4:+33177271	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACTCCTAATAATCCATTTAT+TGG	-	Chr4:33177140-33177159	MsG0480020064.01.T01:CDS	25.0%
!!	TTTTTATTCAAGCATGATCA+TGG	-	Chr4:33177062-33177081	MsG0480020064.01.T01:CDS	25.0%
	CCTAATAATCCATTTATTGG+TGG	-	Chr4:33177143-33177162	MsG0480020064.01.T01:CDS	30.0%
	TCATGCTTGAATAAAAACTG+CGG	+	Chr4:33177059-33177078	None:intergenic	30.0%
!!	CCACCAATAAATGGATTATT+AGG	+	Chr4:33177146-33177165	None:intergenic	30.0%
	TTAGGATTTCCACCGAAAAT+CGG	-	Chr4:33177113-33177132	MsG0480020064.01.T01:CDS	35.0%
	AAACTACATTACCCTGGCAT+TGG	-	Chr4:33177086-33177105	MsG0480020064.01.T01:CDS	40.0%
	AATCCTAAATGTCCAATGCC+AGG	+	Chr4:33177101-33177120	None:intergenic	40.0%
	ATCCTAAATGTCCAATGCCA+GGG	+	Chr4:33177100-33177119	None:intergenic	40.0%
	CATGGTAAACTACATTACCC+TGG	-	Chr4:33177080-33177099	MsG0480020064.01.T01:CDS	40.0%
	TAGGATTTCCACCGAAAATC+GGG	-	Chr4:33177114-33177133	MsG0480020064.01.T01:CDS	40.0%
	TCAGGTCATAGTTATGTTCC+CGG	-	Chr4:33177221-33177240	MsG0480020064.01.T01:CDS	40.0%
	TCTTAGCATTAGCAATGCTG+CGG	+	Chr4:33177010-33177029	None:intergenic	40.0%
!	AATCCATTTATTGGTGGCAC+TGG	-	Chr4:33177149-33177168	MsG0480020064.01.T01:CDS	40.0%
!	TTAGGAGTAAGCCCGATTTT+CGG	+	Chr4:33177128-33177147	None:intergenic	40.0%
	AACAAATGTGTCATCGCCAC+CGG	+	Chr4:33177243-33177262	None:intergenic	45.0%
	GACACATTTGTTCCAAACCC+TGG	-	Chr4:33177251-33177270	MsG0480020064.01.T01:CDS	45.0%
!	GATTTGGAACCTCAAAGCCA+GGG	+	Chr4:33177271-33177290	None:intergenic	45.0%
!	TACCCTGGCATTGGACATTT+AGG	-	Chr4:33177095-33177114	MsG0480020064.01.T01:CDS	45.0%
	ACAAATGTGTCATCGCCACC+GGG	+	Chr4:33177242-33177261	None:intergenic	50.0%
	ACATCACAGCAAGCAGTTGC+TGG	-	Chr4:33176984-33177003	MsG0480020064.01.T01:CDS	50.0%
	CCTCCAGTGCCACCAATAAA+TGG	+	Chr4:33177155-33177174	None:intergenic	50.0%
	CGGTGGTAGAGTTGCATCAA+CGG	-	Chr4:33177310-33177329	MsG0480020064.01.T01:CDS	50.0%
	CTCTTTCTTGTCGTCGCTGT+CGG	+	Chr4:33177036-33177055	None:intergenic	50.0%
	GGTCATAGTTATGTTCCCGG+TGG	-	Chr4:33177224-33177243	MsG0480020064.01.T01:CDS	50.0%
	TTAGGATCAGGAACAGGAGC+AGG	-	Chr4:33177188-33177207	MsG0480020064.01.T01:CDS	50.0%
!	CCATTTATTGGTGGCACTGG+AGG	-	Chr4:33177152-33177171	MsG0480020064.01.T01:CDS	50.0%
!	CTTGCTGTGATGTGACAAGG+AGG	+	Chr4:33176977-33176996	None:intergenic	50.0%
!	GGATTTGGAACCTCAAAGCC+AGG	+	Chr4:33177272-33177291	None:intergenic	50.0%
!	TCAGGTTCGGGTTTAGGATC+AGG	-	Chr4:33177176-33177195	MsG0480020064.01.T01:CDS	50.0%
!	TCGGGTTTAGGATCAGGAAC+AGG	-	Chr4:33177182-33177201	MsG0480020064.01.T01:CDS	50.0%
!!	CTGCTTGCTGTGATGTGACA+AGG	+	Chr4:33176980-33176999	None:intergenic	50.0%
!!	GGCTTTGAGGTTCCAAATCC+CGG	-	Chr4:33177272-33177291	MsG0480020064.01.T01:CDS	50.0%
!!	TGTTCCAAACCCTGGCTTTG+AGG	-	Chr4:33177259-33177278	MsG0480020064.01.T01:CDS	50.0%
	GAGGTTCCAAATCCCGGCAA+TGG	-	Chr4:33177278-33177297	MsG0480020064.01.T01:CDS	55.0%
	GGAACCTCAAAGCCAGGGTT+TGG	+	Chr4:33177266-33177285	None:intergenic	55.0%
	GGAGCAGGATCAGGATCATC+AGG	-	Chr4:33177203-33177222	MsG0480020064.01.T01:CDS	55.0%
	GTTCCAAATCCCGGCAATGG+TGG	-	Chr4:33177281-33177300	MsG0480020064.01.T01:CDS	55.0%
	TCAGGAACAGGAGCAGGATC+AGG	-	Chr4:33177194-33177213	MsG0480020064.01.T01:CDS	55.0%
!	ATTGGTGGCACTGGAGGATC+AGG	-	Chr4:33177158-33177177	MsG0480020064.01.T01:CDS	55.0%
!	GGAGGATCAGGTTCGGGTTT+AGG	-	Chr4:33177170-33177189	MsG0480020064.01.T01:CDS	55.0%
!	GGAGTAAGCCCGATTTTCGG+TGG	+	Chr4:33177125-33177144	None:intergenic	55.0%
!	TGGCACTGGAGGATCAGGTT+CGG	-	Chr4:33177163-33177182	MsG0480020064.01.T01:CDS	55.0%
	CCAAATCCCGGCAATGGTGG+TGG	-	Chr4:33177284-33177303	MsG0480020064.01.T01:CDS	60.0%
	CCACCACCATTGCCGGGATT+TGG	+	Chr4:33177287-33177306	None:intergenic	60.0%
!	GGCACTGGAGGATCAGGTTC+GGG	-	Chr4:33177164-33177183	MsG0480020064.01.T01:CDS	60.0%
!!	AATCCCGGCAATGGTGGTGG+CGG	-	Chr4:33177287-33177306	MsG0480020064.01.T01:CDS	60.0%
	ACCGCCGCCACCACCATTGC+CGG	+	Chr4:33177294-33177313	None:intergenic	70.0%
	CCGCCGCCACCACCATTGCC+GGG	+	Chr4:33177293-33177312	None:intergenic	75.0%
!!	CCCGGCAATGGTGGTGGCGG+CGG	-	Chr4:33177290-33177309	MsG0480020064.01.T01:CDS	75.0%
!!	GGCAATGGTGGTGGCGGCGG+TGG	-	Chr4:33177293-33177312	MsG0480020064.01.T01:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	33176924	33177343	33176924	ID=MsG0480020064.01;Name=MsG0480020064.01
Chr4	mRNA	33176924	33177343	33176924	ID=MsG0480020064.01.T01;Parent=MsG0480020064.01;Name=MsG0480020064.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|139
Chr4	exon	33176924	33177343	33176924	ID=MsG0480020064.01.T01:exon:55324;Parent=MsG0480020064.01.T01
Chr4	CDS	33176924	33177343	33176924	ID=MsG0480020064.01.T01:cds;Parent=MsG0480020064.01.T01

Gene Sequence

>MsG0480020064.01.T01

ATGGTTCAAAGAGCATACTCTTTCTTTGCACTTTTTCTCATTTTCAATATCCTCCTTGTCACATCACAGCAAGCAGTTGCTGGCCGCAGCATTGCTAATGCTAAGAACTCCGACAGCGACGACAAGAAAGAGCCGCAGTTTTTATTCAAGCATGATCATGGTAAACTACATTACCCTGGCATTGGACATTTAGGATTTCCACCGAAAATCGGGCTTACTCCTAATAATCCATTTATTGGTGGCACTGGAGGATCAGGTTCGGGTTTAGGATCAGGAACAGGAGCAGGATCAGGATCATCAGGTCATAGTTATGTTCCCGGTGGCGATGACACATTTGTTCCAAACCCTGGCTTTGAGGTTCCAAATCCCGGCAATGGTGGTGGCGGCGGTGGTAGAGTTGCATCAACGGTTCATCCATGA

Protein sequence

>MsG0480020064.01.T01

MVQRAYSFFALFLIFNILLVTSQQAVAGRSIANAKNSDSDDKKEPQFLFKHDHGKLHYPGIGHLGFPPKIGLTPNNPFIGGTGGSGSGLGSGTGAGSGSSGHSYVPGGDDTFVPNPGFEVPNPGNGGGGGGRVASTVHP*