AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020319.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020319.01.T01 MTR_4g047870 80.829 193 18 2 23 207 94 275 3.68e-105 305
MsG0480020319.01.T01 MTR_5g092060 54.955 222 71 4 4 207 78 288 1.20e-76 233
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020319.01.T01 AT3G21700 66.854 178 39 3 39 207 125 291 2.85e-77 234
MsG0480020319.01.T01 AT3G21700 66.292 178 39 4 39 207 125 290 1.93e-75 229
MsG0480020319.01.T01 AT5G54840 53.571 196 70 4 24 210 105 288 4.42e-65 203
MsG0480020319.01.T01 AT3G21700 54.762 126 36 4 39 155 125 238 8.39e-36 127
MsG0480020319.01.T01 AT5G54840 49.524 105 40 3 25 128 106 198 2.01e-23 94.0
MsG0480020319.01.T01 AT5G54840 49.524 105 40 3 25 128 106 198 3.90e-23 93.6

Find 58 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGAAATTGCTATGTTATTA+TGG 0.194244 4:-38841644 MsG0480020319.01.T01:three_prime_UTR
CACGTATATCCTATTGTATC+TGG 0.293441 4:-38844373 MsG0480020319.01.T01:CDS
ACCTGGCTGCTTGCCTGAGC+AGG 0.330688 4:+38844941 None:intergenic
GCCTGCTCAGGCAAGCAGCC+AGG 0.335224 4:-38844942 MsG0480020319.01.T01:intron
CACCGACTGCTTGCCTGCTC+AGG 0.346195 4:-38844954 MsG0480020319.01.T01:CDS
ATGGTCCATTGCATATCAAT+AGG 0.376812 4:+38843710 None:intergenic
CTTGGGACAATCGAGTTGTT+AGG 0.397084 4:-38841872 MsG0480020319.01.T01:three_prime_UTR
CTAGTGAGAAATAAAAGAAA+TGG 0.416869 4:-38841758 MsG0480020319.01.T01:three_prime_UTR
GTCGATTCAAACATGTTGTA+AGG 0.417683 4:-38841605 MsG0480020319.01.T01:three_prime_UTR
CCAGCTTCCAATGGCATGTA+AGG 0.422047 4:-38844247 MsG0480020319.01.T01:CDS
CTAAACACACACTAATTCTC+TGG 0.435218 4:+38841728 None:intergenic
CAAGTCGATGCACATTAAAC+AGG 0.439545 4:-38844191 MsG0480020319.01.T01:intron
TTGAACATAGTTATTTCCAT+TGG 0.442336 4:+38841831 None:intergenic
AAGAAGCAAGGAATGGAATC+AGG 0.443034 4:-38843871 MsG0480020319.01.T01:intron
TTTATTTCAGTGTTCTTGGA+TGG 0.463895 4:-38843897 MsG0480020319.01.T01:CDS
TTGTACTTCCCAGATACAAT+AGG 0.464379 4:+38844364 None:intergenic
CAAAACTCTTCGACTTACCT+TGG 0.467610 4:-38842050 MsG0480020319.01.T01:CDS
CTGAATGAAATCATCAAACT+TGG 0.473260 4:+38843736 None:intergenic
TGTTGGGAATGAAAAGGAGC+AGG 0.476518 4:-38844453 MsG0480020319.01.T01:CDS
TTGACTTAAACATCTCTACT+TGG 0.486695 4:+38841906 None:intergenic
CTTAAACATCTCTACTTGGT+CGG 0.493017 4:+38841910 None:intergenic
ACTCCTTCGCGCTGCGAAGG+AGG 0.493460 4:-38845025 MsG0480020319.01.T01:CDS
GAGGAAAGGACCAAATCAAA+TGG 0.496937 4:-38844420 MsG0480020319.01.T01:CDS
GTCACTCCTTCGCGCTGCGA+AGG 0.504957 4:-38845028 MsG0480020319.01.T01:CDS
TGCCTGAGCAGGCAAGCAGT+CGG 0.509410 4:+38844952 None:intergenic
CCTTACATGCCATTGGAAGC+TGG 0.517454 4:+38844247 None:intergenic
GTGGAAAGGAATCTAAATGT+TGG 0.519478 4:-38842024 MsG0480020319.01.T01:CDS
AGATTCCTTTCCACTGTCCA+AGG 0.525285 4:+38842033 None:intergenic
ACTGAGTCCTTACATGCCAT+TGG 0.527947 4:+38844240 None:intergenic
GAAATATGTTGGGAATGAAA+AGG 0.534530 4:-38844459 MsG0480020319.01.T01:CDS
TGGTCCATTGCATATCAATA+GGG 0.535828 4:+38843711 None:intergenic
CATAGACATGTCATTTCCAA+TGG 0.545053 4:-38841847 MsG0480020319.01.T01:three_prime_UTR
GTGTTGCAGGTGAAATATGT+TGG 0.547304 4:-38844470 MsG0480020319.01.T01:intron
CTTCGACTTACCTTGGACAG+TGG 0.548530 4:-38842043 MsG0480020319.01.T01:CDS
CAGGGAGTGGCACAGAGGAA+AGG 0.559881 4:-38844434 MsG0480020319.01.T01:CDS
AGCTCCCTATTGATATGCAA+TGG 0.560194 4:-38843715 MsG0480020319.01.T01:CDS
TGTTGCAGGTGAAATATGTT+GGG 0.562818 4:-38844469 MsG0480020319.01.T01:intron
ACGTATATCCTATTGTATCT+GGG 0.564779 4:-38844372 MsG0480020319.01.T01:CDS
AAATCAAATGGACAAAACAT+TGG 0.567322 4:-38844408 MsG0480020319.01.T01:CDS
GAATGAAAAGGAGCAGGGAG+TGG 0.572722 4:-38844447 MsG0480020319.01.T01:CDS
TATTGTATCTGGGAAGTACA+AGG 0.574671 4:-38844362 MsG0480020319.01.T01:intron
ACTTACCTTGGACAGTGGAA+AGG 0.576381 4:-38842038 MsG0480020319.01.T01:CDS
ATCTCCGGAGCAACGATACA+CGG 0.577259 4:+38844994 None:intergenic
AAGCCTCCTTCGCAGCGCGA+AGG 0.580558 4:+38845022 None:intergenic
ACAGCCGTGTATCGTTGCTC+CGG 0.583287 4:-38844998 MsG0480020319.01.T01:CDS
GTTGGGAATGAAAAGGAGCA+GGG 0.584605 4:-38844452 MsG0480020319.01.T01:CDS
GAGCTCGAAGAATACATCTC+CGG 0.586436 4:+38844979 None:intergenic
ATCAGAAGACCAGCTTCCAA+TGG 0.601240 4:-38844256 MsG0480020319.01.T01:CDS
ATGGTACAAGAAGCAAGGAA+TGG 0.609613 4:-38843878 MsG0480020319.01.T01:CDS
GCAATGGACCATCGCTAGTG+AGG 0.615352 4:-38843699 MsG0480020319.01.T01:intron
CTTGGATGGTACAAGAAGCA+AGG 0.623167 4:-38843883 MsG0480020319.01.T01:CDS
AGCGATTACCTCACTAGCGA+TGG 0.627900 4:+38843691 None:intergenic
GACAAAACATTGGTTGTGAG+TGG 0.635995 4:-38844398 MsG0480020319.01.T01:CDS
ACAGGCAAGAACATATGCAA+AGG 0.636144 4:-38842148 MsG0480020319.01.T01:intron
AGGAGCAGGGAGTGGCACAG+AGG 0.649858 4:-38844439 MsG0480020319.01.T01:CDS
TCAATCATCACACAAAAGTG+TGG 0.652869 4:-38841805 MsG0480020319.01.T01:three_prime_UTR
TGCACTTGAAAAGAACAAAG+TGG 0.664540 4:+38842116 None:intergenic
AATGGCATGTAAGGACTCAG+TGG 0.696623 4:-38844238 MsG0480020319.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAATATAAATTTATAAGA+GGG - Chr4:38843228-38843247 MsG0480020319.01.T01:intron 10.0%
!! GAAATTAAAATCATATAATA+TGG - Chr4:38844209-38844228 MsG0480020319.01.T01:CDS 10.0%
!! TATGAATATAAATTTATAAG+AGG - Chr4:38843227-38843246 MsG0480020319.01.T01:intron 10.0%
!! TTAAGAATAAAAAAATGATT+CGG + Chr4:38843144-38843163 None:intergenic 10.0%
!!! AATGTTTTATTTATATTGAA+GGG - Chr4:38842265-38842284 MsG0480020319.01.T01:intron 10.0%
!! ATCATGTTTATCTTAAAATA+TGG + Chr4:38843300-38843319 None:intergenic 15.0%
!! ATTCATGATCAATTTAATTA+TGG - Chr4:38843918-38843937 MsG0480020319.01.T01:CDS 15.0%
!! CATATACTATAAAGTTAAAT+TGG + Chr4:38843451-38843470 None:intergenic 15.0%
!! TGTTTAATAATCATAATCAT+AGG + Chr4:38843509-38843528 None:intergenic 15.0%
!! TTGTTTGTAAATAATGATTT+GGG + Chr4:38844308-38844327 None:intergenic 15.0%
!! TTTGTTTGTAAATAATGATT+TGG + Chr4:38844309-38844328 None:intergenic 15.0%
!!! ATGTTTTATTTATATTGAAG+GGG - Chr4:38842266-38842285 MsG0480020319.01.T01:intron 15.0%
!!! CAATGTTTTATTTATATTGA+AGG - Chr4:38842264-38842283 MsG0480020319.01.T01:intron 15.0%
!! AAATTAGTAATAGTGTCAAT+AGG + Chr4:38843643-38843662 None:intergenic 20.0%
!! ACTATTACTAATTTCTTTGT+AGG - Chr4:38843649-38843668 MsG0480020319.01.T01:intron 20.0%
!! ACTGAAATAAAACCAAAAAA+TGG + Chr4:38842646-38842665 None:intergenic 20.0%
!! CTATTACTAATTTCTTTGTA+GGG - Chr4:38843650-38843669 MsG0480020319.01.T01:intron 20.0%
!! CTGAAATAAAACCAAAAAAT+GGG + Chr4:38842645-38842664 None:intergenic 20.0%
!! TTAAGTGTAATTACAAGTTT+CGG + Chr4:38841921-38841940 None:intergenic 20.0%
!!! AATCAGGTAACATATTATTT+TGG - Chr4:38842697-38842716 MsG0480020319.01.T01:intron 20.0%
!!! ATATTTAAGTTGATTCTGTA+AGG - Chr4:38843261-38843280 MsG0480020319.01.T01:intron 20.0%
!!! CAATCTTCTAAAAATTAAGA+TGG + Chr4:38842958-38842977 None:intergenic 20.0%
!!! CACTTTTGATTAAAATTCAA+TGG + Chr4:38842750-38842769 None:intergenic 20.0%
!!! GAAAAACCAAATTTGAAATA+TGG + Chr4:38844096-38844115 None:intergenic 20.0%
!!! TGACTTTTGTCAAAATATTA+TGG - Chr4:38843873-38843892 MsG0480020319.01.T01:CDS 20.0%
!!! TTTTCTGATGTCAATTATAT+TGG - Chr4:38842580-38842599 MsG0480020319.01.T01:intron 20.0%
! AAATCAAATGGACAAAACAT+TGG - Chr4:38842144-38842163 MsG0480020319.01.T01:CDS 25.0%
! AACGATCCATATTTCAAATT+TGG - Chr4:38844087-38844106 MsG0480020319.01.T01:intron 25.0%
! ACGAAATTGCTATGTTATTA+TGG - Chr4:38844908-38844927 MsG0480020319.01.T01:intron 25.0%
! CAAAGCAAGATAAAAAATCA+TGG + Chr4:38844988-38845007 None:intergenic 25.0%
! CATTTGAACATTGAATTAAC+AGG - Chr4:38844386-38844405 MsG0480020319.01.T01:CDS 25.0%
! CTAGTGAGAAATAAAAGAAA+TGG - Chr4:38844794-38844813 MsG0480020319.01.T01:intron 25.0%
! TACATGTAAGACTATCAATT+TGG - Chr4:38843390-38843409 MsG0480020319.01.T01:intron 25.0%
! TAGGAATGACAAAACAATAA+AGG + Chr4:38843490-38843509 None:intergenic 25.0%
! TCATATACTTACCAAAAAAC+TGG + Chr4:38841815-38841834 None:intergenic 25.0%
! TTATTTACCAATTTGGTCAA+TGG + Chr4:38843984-38844003 None:intergenic 25.0%
! TTTATTTATATTGAAGGGGA+TGG - Chr4:38842270-38842289 MsG0480020319.01.T01:intron 25.0%
!! AAATGAATTGGATACTGATT+TGG - Chr4:38841744-38841763 MsG0480020319.01.T01:three_prime_UTR 25.0%
!! AATGTTTTGTCCATTTGATT+TGG + Chr4:38842145-38842164 None:intergenic 25.0%
!! AGATTTTCAGAATCAATGAT+GGG + Chr4:38844558-38844577 None:intergenic 25.0%
!! TAGATTTTCAGAATCAATGA+TGG + Chr4:38844559-38844578 None:intergenic 25.0%
!! TGTAAATAATGATTTGGGTT+GGG + Chr4:38844303-38844322 None:intergenic 25.0%
!! TGTTTTTCACACAAATGAAT+TGG - Chr4:38841732-38841751 MsG0480020319.01.T01:three_prime_UTR 25.0%
!! TTATTCAGCTTTTCTCATAA+AGG + Chr4:38843425-38843444 None:intergenic 25.0%
!! TTGAACATAGTTATTTCCAT+TGG + Chr4:38844724-38844743 None:intergenic 25.0%
!! TTGTAAATAATGATTTGGGT+TGG + Chr4:38844304-38844323 None:intergenic 25.0%
!!! AAAAACTGGTTTTTCCAATT+TGG + Chr4:38841801-38841820 None:intergenic 25.0%
!!! AATTGGAAAAACCAGTTTTT+TGG - Chr4:38841801-38841820 MsG0480020319.01.T01:three_prime_UTR 25.0%
!!! GATTTTTTATCTTGCTTTGA+AGG - Chr4:38844989-38845008 MsG0480020319.01.T01:CDS 25.0%
!!! TAACAACGTTTTATTCCAAA+AGG - Chr4:38843338-38843357 MsG0480020319.01.T01:intron 25.0%
!!! TAGAGTGTTATTTACCAATT+TGG + Chr4:38843991-38844010 None:intergenic 25.0%
AAGATTGAAAATCTGTCTTG+AGG - Chr4:38842971-38842990 MsG0480020319.01.T01:intron 30.0%
AAGTCAAAAATCATCACTCT+TGG - Chr4:38844662-38844681 MsG0480020319.01.T01:intron 30.0%
ACGTATATCCTATTGTATCT+GGG - Chr4:38842180-38842199 MsG0480020319.01.T01:intron 30.0%
ACTAAAAAAGGACCAAATTG+TGG + Chr4:38843776-38843795 None:intergenic 30.0%
AGGTAACGTGTAAAGAAATA+GGG + Chr4:38843623-38843642 None:intergenic 30.0%
AGTAGTCATTAGACTAAGAT+TGG + Chr4:38844148-38844167 None:intergenic 30.0%
AGTCAAAAATCATCACTCTT+GGG - Chr4:38844663-38844682 MsG0480020319.01.T01:intron 30.0%
AGTCTTACATGTAGATTTGT+AGG + Chr4:38843383-38843402 None:intergenic 30.0%
ATAATTTCTCGCAATTACTC+AGG + Chr4:38842534-38842553 None:intergenic 30.0%
ATTATTAATGTGTGAGTGTG+TGG + Chr4:38844051-38844070 None:intergenic 30.0%
CTGAATGAAATCATCAAACT+TGG + Chr4:38842819-38842838 None:intergenic 30.0%
GAAATATGTTGGGAATGAAA+AGG - Chr4:38842093-38842112 MsG0480020319.01.T01:CDS 30.0%
TAATTTCTCGCAATTACTCA+GGG + Chr4:38842533-38842552 None:intergenic 30.0%
TAGGTAACGTGTAAAGAAAT+AGG + Chr4:38843624-38843643 None:intergenic 30.0%
TCATTTGTGTGAAAAACAGA+TGG + Chr4:38841730-38841749 None:intergenic 30.0%
TCTAATTGGAACACTATATG+CGG + Chr4:38843700-38843719 None:intergenic 30.0%
TTGACTTAAACATCTCTACT+TGG + Chr4:38844649-38844668 None:intergenic 30.0%
TTGTTGAAAAAATGTGTTGC+AGG - Chr4:38842069-38842088 MsG0480020319.01.T01:CDS 30.0%
TTTATTTCAGTGTTCTTGGA+TGG - Chr4:38842655-38842674 MsG0480020319.01.T01:intron 30.0%
! ATTAGTTGTGTAACTTGTGT+CGG - Chr4:38841961-38841980 MsG0480020319.01.T01:exon 30.0%
! TTATGGAATTGAACTCCTTT+TGG + Chr4:38843356-38843375 None:intergenic 30.0%
! TTGATGATCATTGATCCTAA+GGG - Chr4:38844266-38844285 MsG0480020319.01.T01:CDS 30.0%
! TTTGATGATCATTGATCCTA+AGG - Chr4:38844265-38844284 MsG0480020319.01.T01:CDS 30.0%
!! ACTGCTTATAATGGATTATG+GGG + Chr4:38844236-38844255 None:intergenic 30.0%
!! CTTTCTAGTTAGTGTATGTT+TGG - Chr4:38842888-38842907 MsG0480020319.01.T01:intron 30.0%
!! GACTGCTTATAATGGATTAT+GGG + Chr4:38844237-38844256 None:intergenic 30.0%
!! TCACTCAAAATTAGCTTGTT+GGG - Chr4:38841769-38841788 MsG0480020319.01.T01:three_prime_UTR 30.0%
!! TGACTGCTTATAATGGATTA+TGG + Chr4:38844238-38844257 None:intergenic 30.0%
!! TTCACTCAAAATTAGCTTGT+TGG - Chr4:38841768-38841787 MsG0480020319.01.T01:three_prime_UTR 30.0%
!!! CACAAAAGTGTGGTTTTAAA+TGG - Chr4:38844757-38844776 MsG0480020319.01.T01:intron 30.0%
!!! GAAGAATCATTCCCATTTTT+TGG - Chr4:38842631-38842650 MsG0480020319.01.T01:intron 30.0%
!!! TGGTTTTATTTCAGTGTTCT+TGG - Chr4:38842651-38842670 MsG0480020319.01.T01:intron 30.0%
!!! TTATGGTTTTGTCAAACTCA+CGG - Chr4:38844925-38844944 MsG0480020319.01.T01:intron 30.0%
AAATTCAGAATGTGTGTAGC+CGG - Chr4:38842392-38842411 MsG0480020319.01.T01:intron 35.0%
AACAGCACATGCACTAAAAA+AGG + Chr4:38843788-38843807 None:intergenic 35.0%
AAGGGATTGATAGGATTCTT+AGG - Chr4:38843578-38843597 MsG0480020319.01.T01:intron 35.0%
AATTTCTCGCAATTACTCAG+GGG + Chr4:38842532-38842551 None:intergenic 35.0%
ACATCGATGGTAATTGGATA+TGG - Chr4:38842416-38842435 MsG0480020319.01.T01:intron 35.0%
ACCAAATTGTGGTATATGCA+TGG + Chr4:38843765-38843784 None:intergenic 35.0%
ATGGTCCATTGCATATCAAT+AGG + Chr4:38842845-38842864 None:intergenic 35.0%
CAATGTCAATTCAGGTGAAA+GGG - Chr4:38843560-38843579 MsG0480020319.01.T01:intron 35.0%
CACGTATATCCTATTGTATC+TGG - Chr4:38842179-38842198 MsG0480020319.01.T01:intron 35.0%
CATCGATGGTAATTGGATAT+GGG - Chr4:38842417-38842436 MsG0480020319.01.T01:intron 35.0%
CCAAATTGTGGTATATGCAT+GGG + Chr4:38843764-38843783 None:intergenic 35.0%
CTAAACACACACTAATTCTC+TGG + Chr4:38844827-38844846 None:intergenic 35.0%
CTTAAACATCTCTACTTGGT+CGG + Chr4:38844645-38844664 None:intergenic 35.0%
GGATCAATGATCATCAAACA+TGG + Chr4:38844263-38844282 None:intergenic 35.0%
GGTAACGTGTAAAGAAATAG+GGG + Chr4:38843622-38843641 None:intergenic 35.0%
GTAGATTTGTAGGCTTCTTA+TGG + Chr4:38843373-38843392 None:intergenic 35.0%
GTGAAATCCATTGACCAAAT+TGG - Chr4:38843974-38843993 MsG0480020319.01.T01:intron 35.0%
GTGGAAAGGAATCTAAATGT+TGG - Chr4:38844528-38844547 MsG0480020319.01.T01:intron 35.0%
TAACTACATTAGTCCGATCT+CGG + Chr4:38842444-38842463 None:intergenic 35.0%
TCAATCATCACACAAAAGTG+TGG - Chr4:38844747-38844766 MsG0480020319.01.T01:intron 35.0%
TCATGATTTCAGTGAAAACG+CGG + Chr4:38843043-38843062 None:intergenic 35.0%
TGCACTTGAAAAGAACAAAG+TGG + Chr4:38844439-38844458 None:intergenic 35.0%
TGGTCCATTGCATATCAATA+GGG + Chr4:38842844-38842863 None:intergenic 35.0%
TGTGTTCAATCTATGAAGCA+CGG - Chr4:38841845-38841864 MsG0480020319.01.T01:three_prime_UTR 35.0%
TGTTGCAGGTGAAATATGTT+GGG - Chr4:38842083-38842102 MsG0480020319.01.T01:CDS 35.0%
TTAGTGTATGTTTGGTTGAG+CGG - Chr4:38842896-38842915 MsG0480020319.01.T01:intron 35.0%
TTCAGTTGCAATGTCAATTC+AGG - Chr4:38843552-38843571 MsG0480020319.01.T01:intron 35.0%
TTGTACTTCCCAGATACAAT+AGG + Chr4:38842191-38842210 None:intergenic 35.0%
! AGCAGGTGATTGATTCTAAT+TGG + Chr4:38843714-38843733 None:intergenic 35.0%
! AGTTGATTCTGTAAGGTTGT+AGG - Chr4:38843268-38843287 MsG0480020319.01.T01:intron 35.0%
! CATAGACATGTCATTTCCAA+TGG - Chr4:38844705-38844724 MsG0480020319.01.T01:intron 35.0%
! GTCGATTCAAACATGTTGTA+AGG - Chr4:38844947-38844966 MsG0480020319.01.T01:CDS 35.0%
! TATTGTATCTGGGAAGTACA+AGG - Chr4:38842190-38842209 MsG0480020319.01.T01:intron 35.0%
!! AATAATGATTTGGGTTGGGT+AGG + Chr4:38844299-38844318 None:intergenic 35.0%
!! TTTTTAGTGCATGTGCTGTT+TGG - Chr4:38843788-38843807 MsG0480020319.01.T01:intron 35.0%
AACTTGGTTCCTATCAACAC+CGG + Chr4:38842803-38842822 None:intergenic 40.0%
AAGAAGCAAGGAATGGAATC+AGG - Chr4:38842681-38842700 MsG0480020319.01.T01:intron 40.0%
ACAGGCAAGAACATATGCAA+AGG - Chr4:38844404-38844423 MsG0480020319.01.T01:CDS 40.0%
AGCTCCCTATTGATATGCAA+TGG - Chr4:38842837-38842856 MsG0480020319.01.T01:intron 40.0%
ATCACCTGCTTATAGCATAG+TGG - Chr4:38843724-38843743 MsG0480020319.01.T01:CDS 40.0%
ATGGTACAAGAAGCAAGGAA+TGG - Chr4:38842674-38842693 MsG0480020319.01.T01:intron 40.0%
ATTGTTGTAGACGGCAATTC+CGG - Chr4:38842781-38842800 MsG0480020319.01.T01:intron 40.0%
CAAAACTCTTCGACTTACCT+TGG - Chr4:38844502-38844521 MsG0480020319.01.T01:intron 40.0%
CAAGTCGATGCACATTAAAC+AGG - Chr4:38842361-38842380 MsG0480020319.01.T01:intron 40.0%
CCCATGCATATACCACAATT+TGG - Chr4:38843761-38843780 MsG0480020319.01.T01:intron 40.0%
CGTTTGTTTGAATTGGCGTT+AGG + Chr4:38843121-38843140 None:intergenic 40.0%
GAGGAAAGGACCAAATCAAA+TGG - Chr4:38842132-38842151 MsG0480020319.01.T01:CDS 40.0%
GATTCGGCGTTTGTTTGAAT+TGG + Chr4:38843128-38843147 None:intergenic 40.0%
GCAATGTCAATTCAGGTGAA+AGG - Chr4:38843559-38843578 MsG0480020319.01.T01:intron 40.0%
GGTGAGTAACACTGTGAATA+TGG - Chr4:38842917-38842936 MsG0480020319.01.T01:intron 40.0%
GTGTCTGGTATATGTGTTCA+AGG - Chr4:38841987-38842006 MsG0480020319.01.T01:CDS 40.0%
GTGTGCTTGATTGTTGTAGA+CGG - Chr4:38842772-38842791 MsG0480020319.01.T01:intron 40.0%
GTGTTGCAGGTGAAATATGT+TGG - Chr4:38842082-38842101 MsG0480020319.01.T01:CDS 40.0%
TTCAGGTGAAAGGGATTGAT+AGG - Chr4:38843569-38843588 MsG0480020319.01.T01:intron 40.0%
TTGTTGGGTGATTGCCAAAT+TGG - Chr4:38841784-38841803 MsG0480020319.01.T01:three_prime_UTR 40.0%
! CATGGACTGACTGCTTATAA+TGG + Chr4:38844245-38844264 None:intergenic 40.0%
! GACAAAACATTGGTTGTGAG+TGG - Chr4:38842154-38842173 MsG0480020319.01.T01:intron 40.0%
!! ACATAGATGTCGATTTGCTC+TGG - Chr4:38844017-38844036 MsG0480020319.01.T01:intron 40.0%
!! ACTGTTCGATTTTGATCCAC+CGG - Chr4:38842495-38842514 MsG0480020319.01.T01:intron 40.0%
!! TGCGAGAAATTATCGACTCT+AGG - Chr4:38842541-38842560 MsG0480020319.01.T01:intron 40.0%
!! TGGTCGGTTGACTTACTTTT+TGG + Chr4:38844629-38844648 None:intergenic 40.0%
AATGGCATGTAAGGACTCAG+TGG - Chr4:38842314-38842333 MsG0480020319.01.T01:intron 45.0%
ACTGAGTCCTTACATGCCAT+TGG + Chr4:38842315-38842334 None:intergenic 45.0%
ACTTACCTTGGACAGTGGAA+AGG - Chr4:38844514-38844533 MsG0480020319.01.T01:intron 45.0%
AGATTCCTTTCCACTGTCCA+AGG + Chr4:38844522-38844541 None:intergenic 45.0%
ATATGCATGGGATCCACAAG+TGG + Chr4:38843752-38843771 None:intergenic 45.0%
ATCAGAAGACCAGCTTCCAA+TGG - Chr4:38842296-38842315 MsG0480020319.01.T01:intron 45.0%
ATTGGATATGGGACCGAGAT+CGG - Chr4:38842428-38842447 MsG0480020319.01.T01:intron 45.0%
CTTGGATGGTACAAGAAGCA+AGG - Chr4:38842669-38842688 MsG0480020319.01.T01:intron 45.0%
GAAAATCTGTCTTGAGGCTG+TGG - Chr4:38842977-38842996 MsG0480020319.01.T01:intron 45.0%
GAGCTCGAAGAATACATCTC+CGG + Chr4:38841576-38841595 None:intergenic 45.0%
GTGATGGCAATGCTGAATGT+GGG + Chr4:38841663-38841682 None:intergenic 45.0%
GTTGGGAATGAAAAGGAGCA+GGG - Chr4:38842100-38842119 MsG0480020319.01.T01:CDS 45.0%
TCCAATTACCATCGATGTGC+CGG + Chr4:38842414-38842433 None:intergenic 45.0%
TGGTTGCAGAGAAGTTTCGA+CGG + Chr4:38841710-38841729 None:intergenic 45.0%
TGTTGGGAATGAAAAGGAGC+AGG - Chr4:38842099-38842118 MsG0480020319.01.T01:CDS 45.0%
! CTTGGGACAATCGAGTTGTT+AGG - Chr4:38844680-38844699 MsG0480020319.01.T01:intron 45.0%
AATCTGTCTTGAGGCTGTGG+CGG - Chr4:38842980-38842999 MsG0480020319.01.T01:intron 50.0%
AGCGATTACCTCACTAGCGA+TGG + Chr4:38842864-38842883 None:intergenic 50.0%
ATCGGGACACGACTAATCTG+AGG + Chr4:38841876-38841895 None:intergenic 50.0%
ATCTCCGGAGCAACGATACA+CGG + Chr4:38841561-38841580 None:intergenic 50.0%
ATTCAGCATTGCCATCACCG+CGG - Chr4:38841665-38841684 MsG0480020319.01.T01:intron 50.0%
CCAGCTTCCAATGGCATGTA+AGG - Chr4:38842305-38842324 MsG0480020319.01.T01:intron 50.0%
CCTTACATGCCATTGGAAGC+TGG + Chr4:38842308-38842327 None:intergenic 50.0%
CTGTACGTAGCATTAGCACC+TGG + Chr4:38841631-38841650 None:intergenic 50.0%
CTTCGACTTACCTTGGACAG+TGG - Chr4:38844509-38844528 MsG0480020319.01.T01:intron 50.0%
GAATGAAAAGGAGCAGGGAG+TGG - Chr4:38842105-38842124 MsG0480020319.01.T01:CDS 50.0%
GAATTCACGCATTCACGCAG+AGG + Chr4:38844185-38844204 None:intergenic 50.0%
GCGTGAATGCGTGAATTCAC+TGG - Chr4:38844187-38844206 MsG0480020319.01.T01:intron 50.0%
GGCACCACTATGCTATAAGC+AGG + Chr4:38843731-38843750 None:intergenic 50.0%
TTGCAGAGAAGTTTCGACGG+TGG + Chr4:38841707-38841726 None:intergenic 50.0%
! ATTACTCAGGGGCGCATAAG+TGG + Chr4:38842521-38842540 None:intergenic 50.0%
! GATTAGTCGTGTCCCGATGT+CGG - Chr4:38841878-38841897 MsG0480020319.01.T01:three_prime_UTR 50.0%
! GGTGATGGCAATGCTGAATG+TGG + Chr4:38841664-38841683 None:intergenic 50.0%
!! AACTTGTGTCGGCTTGTGTC+TGG - Chr4:38841972-38841991 MsG0480020319.01.T01:CDS 50.0%
!! ACGGCAATTCCGGTGTTGAT+AGG - Chr4:38842791-38842810 MsG0480020319.01.T01:intron 50.0%
!! GTCGTGATTTTGACCTCACG+TGG + Chr4:38843014-38843033 None:intergenic 50.0%
!! TAGCATAGTGGTGCCACTTG+TGG - Chr4:38843736-38843755 MsG0480020319.01.T01:CDS 50.0%
ACAGCCGTGTATCGTTGCTC+CGG - Chr4:38841554-38841573 MsG0480020319.01.T01:three_prime_UTR 55.0%
GAATCCAACAACGAAGCCCG+CGG + Chr4:38841685-38841704 None:intergenic 55.0%
GCAATGGACCATCGCTAGTG+AGG - Chr4:38842853-38842872 MsG0480020319.01.T01:intron 55.0%
GCCGGCACATCGATGGTAAT+TGG - Chr4:38842410-38842429 MsG0480020319.01.T01:intron 55.0%
TGGGTAGGCTTGTCTCCCTT+AGG + Chr4:38844284-38844303 None:intergenic 55.0%
TTCAGCATTGCCATCACCGC+GGG - Chr4:38841666-38841685 MsG0480020319.01.T01:intron 55.0%
AACAACGAAGCCCGCGGTGA+TGG + Chr4:38841679-38841698 None:intergenic 60.0%
ATCACCGCGGGCTTCGTTGT+TGG - Chr4:38841678-38841697 MsG0480020319.01.T01:intron 60.0%
CAGGGAGTGGCACAGAGGAA+AGG - Chr4:38842118-38842137 MsG0480020319.01.T01:CDS 60.0%
CGACACGACGTGTCCGACAT+CGG + Chr4:38841894-38841913 None:intergenic 60.0%
CGATGTCGGACACGTCGTGT+CGG - Chr4:38841892-38841911 MsG0480020319.01.T01:three_prime_UTR 60.0%
GACACGACGTGTCCGACATC+GGG + Chr4:38841893-38841912 None:intergenic 60.0%
GTGTGTAGCCGGCACATCGA+TGG - Chr4:38842403-38842422 MsG0480020319.01.T01:intron 60.0%
TGCCTGAGCAGGCAAGCAGT+CGG + Chr4:38841603-38841622 None:intergenic 60.0%
TGTCTTGAGGCTGTGGCGGT+GGG - Chr4:38842984-38843003 MsG0480020319.01.T01:intron 60.0%
AAGCCTCCTTCGCAGCGCGA+AGG + Chr4:38841533-38841552 None:intergenic 65.0%
ACCTGGCTGCTTGCCTGAGC+AGG + Chr4:38841614-38841633 None:intergenic 65.0%
ACTCCTTCGCGCTGCGAAGG+AGG - Chr4:38841527-38841546 MsG0480020319.01.T01:three_prime_UTR 65.0%
AGGAGCAGGGAGTGGCACAG+AGG - Chr4:38842113-38842132 MsG0480020319.01.T01:CDS 65.0%
CTGTCTTGAGGCTGTGGCGG+TGG - Chr4:38842983-38843002 MsG0480020319.01.T01:intron 65.0%
GGCGGTGGGAATTCCACGTG+AGG - Chr4:38842998-38843017 MsG0480020319.01.T01:intron 65.0%
GTCACTCCTTCGCGCTGCGA+AGG - Chr4:38841524-38841543 MsG0480020319.01.T01:three_prime_UTR 65.0%
! CACCGACTGCTTGCCTGCTC+AGG - Chr4:38841598-38841617 MsG0480020319.01.T01:three_prime_UTR 65.0%
! CTCAGGGGCGCATAAGTGGC+CGG + Chr4:38842517-38842536 None:intergenic 65.0%
GCCTGCTCAGGCAAGCAGCC+AGG - Chr4:38841610-38841629 MsG0480020319.01.T01:three_prime_UTR 70.0%
! AGGGGCGCATAAGTGGCCGG+TGG + Chr4:38842514-38842533 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 38841521 38845053 38841521 ID=MsG0480020319.01;Name=MsG0480020319.01
Chr4 mRNA 38841521 38845053 38841521 ID=MsG0480020319.01.T01;Parent=MsG0480020319.01;Name=MsG0480020319.01.T01;_AED=0.47;_eAED=0.53;_QI=0|0.5|0.57|1|0.5|0.28|7|420|220
Chr4 exon 38841521 38841664 38841521 ID=MsG0480020319.01.T01:exon:3913;Parent=MsG0480020319.01.T01
Chr4 exon 38841690 38842166 38841690 ID=MsG0480020319.01.T01:exon:3912;Parent=MsG0480020319.01.T01
Chr4 exon 38843700 38843761 38843700 ID=MsG0480020319.01.T01:exon:3911;Parent=MsG0480020319.01.T01
Chr4 exon 38843872 38843942 38843872 ID=MsG0480020319.01.T01:exon:3910;Parent=MsG0480020319.01.T01
Chr4 exon 38844192 38844288 38844192 ID=MsG0480020319.01.T01:exon:3909;Parent=MsG0480020319.01.T01
Chr4 exon 38844363 38844483 38844363 ID=MsG0480020319.01.T01:exon:3908;Parent=MsG0480020319.01.T01
Chr4 exon 38844943 38845053 38844943 ID=MsG0480020319.01.T01:exon:3907;Parent=MsG0480020319.01.T01
Chr4 CDS 38844943 38845053 38844943 ID=MsG0480020319.01.T01:cds;Parent=MsG0480020319.01.T01
Chr4 CDS 38844363 38844483 38844363 ID=MsG0480020319.01.T01:cds;Parent=MsG0480020319.01.T01
Chr4 CDS 38844192 38844288 38844192 ID=MsG0480020319.01.T01:cds;Parent=MsG0480020319.01.T01
Chr4 CDS 38843872 38843942 38843872 ID=MsG0480020319.01.T01:cds;Parent=MsG0480020319.01.T01
Chr4 CDS 38843700 38843761 38843700 ID=MsG0480020319.01.T01:cds;Parent=MsG0480020319.01.T01
Chr4 CDS 38841966 38842166 38841966 ID=MsG0480020319.01.T01:cds;Parent=MsG0480020319.01.T01
Chr4 three_prime_UTR 38841690 38841965 38841690 ID=MsG0480020319.01.T01:three_prime_utr;Parent=MsG0480020319.01.T01
Chr4 three_prime_UTR 38841521 38841664 38841521 ID=MsG0480020319.01.T01:three_prime_utr;Parent=MsG0480020319.01.T01
Gene Sequence

>MsG0480020319.01.T01

ATGGTCACTCCTTCGCGCTGCGAAGGAGGCTTCACAGCCGTGTATCGTTGCTCCGGAGATGTATTCTTCGAGCTCTGCACCGACTGCTTGCCTGCTCAGGCAAGCAGCCAGGTGAAATATGTTGGGAATGAAAAGGAGCAGGGAGTGGCACAGAGGAAAGGACCAAATCAAATGGACAAAACATTGGTTGTGAGTGGAGCACGTATATCCTATTGTATCTGGGAAGTACAAGGGGATGGAAAATCAGAAGACCAGCTTCCAATGGCATGTAAGGACTCAGTGGCAATTTTGATCATGTTTGATCTTACAAGTCGATGCACATTAAACAGAAGAATCATTCCCATTTTTTGGTTTTATTTCAGTGTTCTTGGATGGTACAAGAAGCAAGGAATGGAATCAGGAACCAAGTTTGATGATTTCATTCAGCTCCCTATTGATATGCAATGGACCATCGCTAGTGAGGCAAGAACATATGCAAAGGCATTGAATGCCACTTTGTTCTTTTCAAGTGCAACATACAACATCAATGTCAACAAGATCTTCAAGTTCATCACTGCAAAACTCTTCGACTTACCTTGGACAGTGGAAAGGAATCTAAATGTTGGAGAACCCATCATTGATTCTGAAAATCTATGTGTACATTTACATAGAAGAAAATGTTGA
Protein sequence

>MsG0480020319.01.T01

MVTPSRCEGGFTAVYRCSGDVFFELCTDCLPAQASSQVKYVGNEKEQGVAQRKGPNQMDKTLVVSGARISYCIWEVQGDGKSEDQLPMACKDSVAILIMFDLTSRCTLNRRIIPIFWFYFSVLGWYKKQGMESGTKFDDFIQLPIDMQWTIASEARTYAKALNATLFFSSATYNINVNKIFKFITAKLFDLPWTVERNLNVGEPIIDSENLCVHLHRRKC*