AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020375.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020375.01.T01 MTR_8g102530 97.802 182 4 0 1 182 1 182 1.34e-132 369
MsG0480020375.01.T01 MTR_8g102530 93.407 182 3 1 1 182 1 173 4.33e-123 344
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020375.01.T01 AT5G15750 79.558 181 37 0 1 181 1 181 2.32e-108 307

Find 65 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATGGTTATCTCCTTTAA+TGG 0.197442 4:+40082068 MsG0480020375.01.T01:three_prime_UTR
ATACAGGGGTTAATGAAATT+TGG 0.202523 4:+40082126 MsG0480020375.01.T01:three_prime_UTR
GCTGGGTCTGTAATTGTATT+TGG 0.229997 4:-40081840 None:intergenic
ATTTCATTAACCCCTGTATA+TGG 0.259342 4:-40082122 None:intergenic
TGATATGACTGATAAGCTTT+TGG 0.287922 4:+40080291 MsG0480020375.01.T01:CDS
TCCCTCCCTCCCTCCCTCTA+AGG 0.341635 4:+40079712 MsG0480020375.01.T01:five_prime_UTR
GTATGGTTATCTCCTTTAAT+GGG 0.342327 4:+40082069 MsG0480020375.01.T01:three_prime_UTR
ACCAAGACTGTTTGAATTTG+TGG 0.357222 4:+40082030 MsG0480020375.01.T01:three_prime_UTR
AAAAGAATCATTCCATATAC+AGG 0.364481 4:+40082110 MsG0480020375.01.T01:three_prime_UTR
AGCTGTAACTTATATTGAAC+AGG 0.366283 4:+40081800 MsG0480020375.01.T01:CDS
AAGCACCTTTCTCTTTATCT+TGG 0.381545 4:-40081913 None:intergenic
CTATGATTTAATGAATTAGT+TGG 0.383623 4:+40081959 MsG0480020375.01.T01:exon
ACGTAGGCTCTCAACTGTTT+TGG 0.388493 4:+40081746 MsG0480020375.01.T01:CDS
CTTCCATGTTTCTAGTTACA+AGG 0.426456 4:-40081866 None:intergenic
ATCAACTCGGAAAGGATCCT+TGG 0.429618 4:-40080272 None:intergenic
GAGGGAGGGAGGGAAGACAG+TGG 0.440075 4:-40079703 None:intergenic
GGTTAATGAAATTTGGATGT+AGG 0.444873 4:+40082133 MsG0480020375.01.T01:three_prime_UTR
TTTCTAGTTACAAGGTAAGC+TGG 0.444914 4:-40081858 None:intergenic
ACATAGTGTGATGCTTTGCC+TGG 0.454332 4:-40080922 None:intergenic
TCCACAAATTCAAACAGTCT+TGG 0.459333 4:-40082031 None:intergenic
TCTCTTCAGCTGCCGTCTAC+CGG 0.472268 4:+40079679 None:intergenic
AGAACGGGAACGCACCTTAG+AGG 0.491710 4:-40079726 None:intergenic
TAACCGTGTCAGCCTTCTGC+AGG 0.495522 4:+40080952 MsG0480020375.01.T01:CDS
ATGCCGGATGGTTCAGAAGT+TGG 0.503050 4:+40080225 MsG0480020375.01.T01:CDS
ATGCAGCGCTACCATGTTAC+CGG 0.507249 4:+40080051 MsG0480020375.01.T01:CDS
AGGGAGGGAGGGAAGACAGT+GGG 0.515293 4:-40079702 None:intergenic
ACCTTAGAGGGAGGGAGGGA+GGG 0.522954 4:-40079713 None:intergenic
TTCTAGTTACAAGGTAAGCT+GGG 0.525625 4:-40081857 None:intergenic
AGTGTGATGCTTTGCCTGGT+TGG 0.526362 4:-40080918 None:intergenic
GAAGACAGTGGGCCGGTAGA+CGG 0.529553 4:-40079691 None:intergenic
TCAACTCGGAAAGGATCCTT+GGG 0.536281 4:-40080271 None:intergenic
CACCTTAGAGGGAGGGAGGG+AGG 0.545374 4:-40079714 None:intergenic
GCGCTACCATGTTACCGGTC+GGG 0.546021 4:+40080056 MsG0480020375.01.T01:CDS
AATCGTCCCGACCGGTAACA+TGG 0.550659 4:-40080062 None:intergenic
ACATGGAAGACATGATTACA+TGG 0.553951 4:+40081880 MsG0480020375.01.T01:CDS
TTCTTCCAAGATAAAGAGAA+AGG 0.564085 4:+40081908 MsG0480020375.01.T01:CDS
GAACGGGAACGCACCTTAGA+GGG 0.568621 4:-40079725 None:intergenic
TTGGAATGGAAGAGAGAAGG+GGG 0.573733 4:+40080009 MsG0480020375.01.T01:CDS
ATACCAACTTCTGAACCATC+CGG 0.582879 4:-40080228 None:intergenic
AACGCACCTTAGAGGGAGGG+AGG 0.585087 4:-40079718 None:intergenic
AGGGAGGGAAGACAGTGGGC+CGG 0.585433 4:-40079698 None:intergenic
AAAGAATCATTCCATATACA+GGG 0.591714 4:+40082111 MsG0480020375.01.T01:three_prime_UTR
TTACCTGCAGAAGGCTGACA+CGG 0.596614 4:-40080955 None:intergenic
ATAGGTATTCGAGTATATGC+CGG 0.597856 4:+40080209 MsG0480020375.01.T01:intron
GAACAGGGACATGTTCGAGT+TGG 0.599994 4:+40081816 MsG0480020375.01.T01:CDS
GGTATACACGGTGAAACAGA+TGG 0.602726 4:+40080246 MsG0480020375.01.T01:CDS
GCTTCAGTACAATGACAAGT+TGG 0.608210 4:+40081932 MsG0480020375.01.T01:CDS
GGAAGACATGATTACATGGG+TGG 0.620356 4:+40081884 MsG0480020375.01.T01:CDS
TCAGTCATATCAACTCGGAA+AGG 0.622552 4:-40080280 None:intergenic
AGCTAAAGCATCATGAGCAT+AGG 0.623806 4:+40079965 MsG0480020375.01.T01:CDS
GGTTCAGAAGTTGGTATACA+CGG 0.626707 4:+40080234 MsG0480020375.01.T01:CDS
CGGGAACGCACCTTAGAGGG+AGG 0.632501 4:-40079722 None:intergenic
AGCGCTACCATGTTACCGGT+CGG 0.636484 4:+40080055 MsG0480020375.01.T01:CDS
ACGCACCTTAGAGGGAGGGA+GGG 0.637958 4:-40079717 None:intergenic
TTTGGAATGGAAGAGAGAAG+GGG 0.646764 4:+40080008 MsG0480020375.01.T01:CDS
GGTGAAACAGATGGATCCCA+AGG 0.652966 4:+40080255 MsG0480020375.01.T01:CDS
TTACCTTGTAACTAGAAACA+TGG 0.656200 4:+40081863 MsG0480020375.01.T01:CDS
ATATGGGTGTGATTCCAACC+AGG 0.661105 4:+40080904 MsG0480020375.01.T01:CDS
GCTTATCAGTCATATCAACT+CGG 0.663758 4:-40080285 None:intergenic
GGGAACGCACCTTAGAGGGA+GGG 0.665371 4:-40079721 None:intergenic
GTATTCGAGTATATGCCGGA+TGG 0.685170 4:+40080213 MsG0480020375.01.T01:CDS
CATGGAAGACATGATTACAT+GGG 0.691863 4:+40081881 MsG0480020375.01.T01:CDS
AAGAATCATTCCATATACAG+GGG 0.707301 4:+40082112 MsG0480020375.01.T01:three_prime_UTR
GCTGTAACTTATATTGAACA+GGG 0.722848 4:+40081801 MsG0480020375.01.T01:CDS
GATGACAGTGAATAACAATG+AGG 0.742815 4:+40079941 MsG0480020375.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATTTGTTTCATAATTA+TGG + Chr4:40080375-40080394 MsG0480020375.01.T01:intron 10.0%
!!! AATCAAATTATTTTAAGATA+AGG - Chr4:40079893-40079912 None:intergenic 10.0%
!!! ATTTTCATATGAAAAAAATA+AGG + Chr4:40081045-40081064 MsG0480020375.01.T01:intron 10.0%
!!! TTTTCATATGAAAAAAATAA+GGG + Chr4:40081046-40081065 MsG0480020375.01.T01:intron 10.0%
!! TAAGTTATATAACAAAACTA+TGG - Chr4:40080683-40080702 None:intergenic 15.0%
!! TTTATTACATTCACATAAAA+TGG - Chr4:40081420-40081439 None:intergenic 15.0%
!! TTTCATATGAAAAAAATAAG+GGG + Chr4:40081047-40081066 MsG0480020375.01.T01:intron 15.0%
!!! TATTTTTCTTTGTTTTAGTT+TGG + Chr4:40080134-40080153 MsG0480020375.01.T01:intron 15.0%
!! AAAAAAGAATTTCCCATTAA+AGG - Chr4:40082084-40082103 None:intergenic 20.0%
!! AAATGAAATGTGATGAAAAT+AGG + Chr4:40080615-40080634 MsG0480020375.01.T01:intron 20.0%
!! AGTTAATAAAGTTGAAACAT+TGG + Chr4:40081596-40081615 MsG0480020375.01.T01:intron 20.0%
!! CTATGATTTAATGAATTAGT+TGG + Chr4:40081959-40081978 MsG0480020375.01.T01:exon 20.0%
!! TAAATTATATACAAGTTGCA+GGG + Chr4:40080546-40080565 MsG0480020375.01.T01:intron 20.0%
!!! TATTTTCATCACATTTCATT+TGG - Chr4:40080616-40080635 None:intergenic 20.0%
!!! TTTTATGACAGTTACAATAT+GGG + Chr4:40080888-40080907 MsG0480020375.01.T01:intron 20.0%
!!! TTTTTATGACAGTTACAATA+TGG + Chr4:40080887-40080906 MsG0480020375.01.T01:intron 20.0%
! AAAACAGCTTATGATTAGAA+AGG - Chr4:40079815-40079834 None:intergenic 25.0%
! AAAAGAATCATTCCATATAC+AGG + Chr4:40082110-40082129 MsG0480020375.01.T01:three_prime_UTR 25.0%
! AAACAGCTTATGATTAGAAA+GGG - Chr4:40079814-40079833 None:intergenic 25.0%
! AAAGAATCATTCCATATACA+GGG + Chr4:40082111-40082130 MsG0480020375.01.T01:three_prime_UTR 25.0%
! AGATTATGTCAAAAGAAAGA+TGG - Chr4:40080437-40080456 None:intergenic 25.0%
! ATGACATAAATAGTGAATAC+AGG + Chr4:40080587-40080606 MsG0480020375.01.T01:intron 25.0%
! CATCACAATTCATTCTATTA+AGG - Chr4:40079925-40079944 None:intergenic 25.0%
! GTAAATTATATACAAGTTGC+AGG + Chr4:40080545-40080564 MsG0480020375.01.T01:intron 25.0%
! GTAACTGTCATAAAAAAGAA+AGG - Chr4:40080883-40080902 None:intergenic 25.0%
! GTGGTATATATATTGTAGTA+AGG + Chr4:40081385-40081404 MsG0480020375.01.T01:intron 25.0%
! TATAACTTACAAGTTTGTCT+AGG + Chr4:40080694-40080713 MsG0480020375.01.T01:intron 25.0%
!! ATTCATTTTCTTTGTGGAAT+TGG + Chr4:40080167-40080186 MsG0480020375.01.T01:intron 25.0%
!! ATTGTGGTGGAAATATTAAT+AGG + Chr4:40080191-40080210 MsG0480020375.01.T01:intron 25.0%
!! GACGTATTAATGAACATTTT+TGG + Chr4:40081144-40081163 MsG0480020375.01.T01:intron 25.0%
!! TAAAGTTGAAACATTGGTTA+TGG + Chr4:40081602-40081621 MsG0480020375.01.T01:intron 25.0%
!! TTCATTTTCTTTGTGGAATT+GGG + Chr4:40080168-40080187 MsG0480020375.01.T01:intron 25.0%
!! TTTTATGTTGTTCTTGCATT+TGG + Chr4:40080111-40080130 MsG0480020375.01.T01:intron 25.0%
!!! AACAATCATCCTTTTTGAAA+TGG - Chr4:40080747-40080766 None:intergenic 25.0%
!!! GATGAATTTTTGACTTTGTA+TGG + Chr4:40082052-40082071 MsG0480020375.01.T01:three_prime_UTR 25.0%
!!! GCTTTTCCCTTTTTTAATAT+GGG + Chr4:40079783-40079802 MsG0480020375.01.T01:intron 25.0%
!!! TGAAGAATTTCATTTTTCAG+TGG - Chr4:40081464-40081483 None:intergenic 25.0%
!!! TTTGAGAAAGGTGAATTTTT+TGG + Chr4:40079990-40080009 MsG0480020375.01.T01:CDS 25.0%
AAAACTAACAAGACGAGATT+AGG - Chr4:40081637-40081656 None:intergenic 30.0%
AAACTAACAAGACGAGATTA+GGG - Chr4:40081636-40081655 None:intergenic 30.0%
AACAAAATTCGGAGCAAATT+AGG - Chr4:40081713-40081732 None:intergenic 30.0%
AAGAATCATTCCATATACAG+GGG + Chr4:40082112-40082131 MsG0480020375.01.T01:three_prime_UTR 30.0%
AATAGCAATAGAAAGGGAAA+GGG - Chr4:40079759-40079778 None:intergenic 30.0%
AGCGAATAATAGCAATAGAA+AGG - Chr4:40079766-40079785 None:intergenic 30.0%
AGCTGTAACTTATATTGAAC+AGG + Chr4:40081800-40081819 MsG0480020375.01.T01:CDS 30.0%
AGTGTCTATAGTTATGGATT+TGG + Chr4:40081119-40081138 MsG0480020375.01.T01:intron 30.0%
ATACAGGGGTTAATGAAATT+TGG + Chr4:40082126-40082145 MsG0480020375.01.T01:three_prime_UTR 30.0%
ATATATTGTAGTAAGGCCAA+GGG + Chr4:40081392-40081411 MsG0480020375.01.T01:intron 30.0%
ATATGAAAAAAATAAGGGGC+AGG + Chr4:40081051-40081070 MsG0480020375.01.T01:intron 30.0%
ATTTCATTAACCCCTGTATA+TGG - Chr4:40082125-40082144 None:intergenic 30.0%
GACTGTCCCATATTAAAAAA+GGG - Chr4:40079792-40079811 None:intergenic 30.0%
GCGAATAATAGCAATAGAAA+GGG - Chr4:40079765-40079784 None:intergenic 30.0%
GCTGTAACTTATATTGAACA+GGG + Chr4:40081801-40081820 MsG0480020375.01.T01:CDS 30.0%
GGTTAATGAAATTTGGATGT+AGG + Chr4:40082133-40082152 MsG0480020375.01.T01:three_prime_UTR 30.0%
GTAATCATCTAACATTAGCA+TGG + Chr4:40081234-40081253 MsG0480020375.01.T01:intron 30.0%
GTATGGTTATCTCCTTTAAT+GGG + Chr4:40082069-40082088 MsG0480020375.01.T01:three_prime_UTR 30.0%
GTGTCTATAGTTATGGATTT+GGG + Chr4:40081120-40081139 MsG0480020375.01.T01:intron 30.0%
TAATAGCAATAGAAAGGGAA+AGG - Chr4:40079760-40079779 None:intergenic 30.0%
TAATGAGCAAACAGATAAAG+TGG - Chr4:40081019-40081038 None:intergenic 30.0%
TATATATTGTAGTAAGGCCA+AGG + Chr4:40081391-40081410 MsG0480020375.01.T01:intron 30.0%
TGTATGGTTATCTCCTTTAA+TGG + Chr4:40082068-40082087 MsG0480020375.01.T01:three_prime_UTR 30.0%
TTACCTTGTAACTAGAAACA+TGG + Chr4:40081863-40081882 MsG0480020375.01.T01:CDS 30.0%
TTATCTTTCTTCTAATGCGA+CGG + Chr4:40081530-40081549 MsG0480020375.01.T01:intron 30.0%
TTCTTCCAAGATAAAGAGAA+AGG + Chr4:40081908-40081927 MsG0480020375.01.T01:CDS 30.0%
! GCATGATAGTGAACAAAATT+CGG - Chr4:40081724-40081743 None:intergenic 30.0%
! GCTTTCTTTTTGCTTCTTAT+TGG + Chr4:40080781-40080800 MsG0480020375.01.T01:intron 30.0%
! TGATATGACTGATAAGCTTT+TGG + Chr4:40080291-40080310 MsG0480020375.01.T01:CDS 30.0%
!! CAAGAAAGTGTCTATAGTTA+TGG + Chr4:40081113-40081132 MsG0480020375.01.T01:intron 30.0%
!! TAGCTGATTCATTTTCTTTG+TGG + Chr4:40080161-40080180 MsG0480020375.01.T01:intron 30.0%
!! TTTTTAGCCCAAAACTTAGT+TGG - Chr4:40081556-40081575 None:intergenic 30.0%
!!! ACTAAGTTTTGGGCTAAAAA+TGG + Chr4:40081556-40081575 MsG0480020375.01.T01:intron 30.0%
!!! CAGTTGTTTTAATACAGTTG+AGG - Chr4:40081212-40081231 None:intergenic 30.0%
!!! CGCTTTTCCCTTTTTTAATA+TGG + Chr4:40079782-40079801 MsG0480020375.01.T01:intron 30.0%
!!! GAAAGGTGAATTTTTTGGAA+TGG + Chr4:40079995-40080014 MsG0480020375.01.T01:CDS 30.0%
AAGCACCTTTCTCTTTATCT+TGG - Chr4:40081916-40081935 None:intergenic 35.0%
ACATGGAAGACATGATTACA+TGG + Chr4:40081880-40081899 MsG0480020375.01.T01:CDS 35.0%
ACCAAGACTGTTTGAATTTG+TGG + Chr4:40082030-40082049 MsG0480020375.01.T01:three_prime_UTR 35.0%
ATAGGTATTCGAGTATATGC+CGG + Chr4:40080209-40080228 MsG0480020375.01.T01:intron 35.0%
ATCTAACATTAGCATGGACA+TGG + Chr4:40081240-40081259 MsG0480020375.01.T01:intron 35.0%
ATTATATACAAGTTGCAGGG+TGG + Chr4:40080549-40080568 MsG0480020375.01.T01:intron 35.0%
CATGGAAGACATGATTACAT+GGG + Chr4:40081881-40081900 MsG0480020375.01.T01:CDS 35.0%
GATGACAGTGAATAACAATG+AGG + Chr4:40079941-40079960 MsG0480020375.01.T01:intron 35.0%
GCTTATCAGTCATATCAACT+CGG - Chr4:40080288-40080307 None:intergenic 35.0%
GGACTGTCCCATATTAAAAA+AGG - Chr4:40079793-40079812 None:intergenic 35.0%
TCCACAAATTCAAACAGTCT+TGG - Chr4:40082034-40082053 None:intergenic 35.0%
TTTATCATCGCTTTGTAGCT+AGG + Chr4:40081333-40081352 MsG0480020375.01.T01:intron 35.0%
! AGATACGACACTATGAGTTT+TGG + Chr4:40080494-40080513 MsG0480020375.01.T01:intron 35.0%
! GCCAAGTTATCTAGTTTCAA+GGG + Chr4:40081279-40081298 MsG0480020375.01.T01:intron 35.0%
! TGCCAAGTTATCTAGTTTCA+AGG + Chr4:40081278-40081297 MsG0480020375.01.T01:intron 35.0%
! TTAGACCTGCCATTTCAAAA+AGG + Chr4:40080735-40080754 MsG0480020375.01.T01:intron 35.0%
! TTCTAGTTACAAGGTAAGCT+GGG - Chr4:40081860-40081879 None:intergenic 35.0%
!! ATACCACTTTTCATACGACT+CGG - Chr4:40081372-40081391 None:intergenic 35.0%
!! CTTCCATGTTTCTAGTTACA+AGG - Chr4:40081869-40081888 None:intergenic 35.0%
!! TTTCTAGTTACAAGGTAAGC+TGG - Chr4:40081861-40081880 None:intergenic 35.0%
!!! ATCATCCTTTTTGAAATGGC+AGG - Chr4:40080743-40080762 None:intergenic 35.0%
!!! ATTTTTGGCCTTCAAAGTCT+AGG + Chr4:40081159-40081178 MsG0480020375.01.T01:intron 35.0%
!!! TTTTGGAATGGAAGAGAGAA+GGG + Chr4:40080007-40080026 MsG0480020375.01.T01:CDS 35.0%
!!! TTTTTGGAATGGAAGAGAGA+AGG + Chr4:40080006-40080025 MsG0480020375.01.T01:CDS 35.0%
AACTGACTGCAATGGTTCAT+TGG + Chr4:40080640-40080659 MsG0480020375.01.T01:intron 40.0%
AATAGGTGAACTGACTGCAA+TGG + Chr4:40080632-40080651 MsG0480020375.01.T01:intron 40.0%
AGACTGTTCCTAGACTTTGA+AGG - Chr4:40081170-40081189 None:intergenic 40.0%
ATACCAACTTCTGAACCATC+CGG - Chr4:40080231-40080250 None:intergenic 40.0%
ATTCCGAGTCGTATGAAAAG+TGG + Chr4:40081366-40081385 MsG0480020375.01.T01:intron 40.0%
GACGATGTAATGCAAAGTTG+AGG - Chr4:40081691-40081710 None:intergenic 40.0%
GCCCTTGAAACTAGATAACT+TGG - Chr4:40081283-40081302 None:intergenic 40.0%
GCTGGGTCTGTAATTGTATT+TGG - Chr4:40081843-40081862 None:intergenic 40.0%
GCTTCAGTACAATGACAAGT+TGG + Chr4:40081932-40081951 MsG0480020375.01.T01:CDS 40.0%
GTCACATGAAGGTCAACATA+TGG + Chr4:40081310-40081329 MsG0480020375.01.T01:intron 40.0%
TCAGTCATATCAACTCGGAA+AGG - Chr4:40080283-40080302 None:intergenic 40.0%
TCTTTGTGGAATTGGGATTG+TGG + Chr4:40080175-40080194 MsG0480020375.01.T01:intron 40.0%
TTCACATAAAATGGCACCCT+TGG - Chr4:40081411-40081430 None:intergenic 40.0%
! AGCTAAAGCATCATGAGCAT+AGG + Chr4:40079965-40079984 MsG0480020375.01.T01:CDS 40.0%
! GGGCAAATTTTGTCACATGA+AGG + Chr4:40081299-40081318 MsG0480020375.01.T01:intron 40.0%
! TTTGGAATGGAAGAGAGAAG+GGG + Chr4:40080008-40080027 MsG0480020375.01.T01:CDS 40.0%
!! GGTTCAGAAGTTGGTATACA+CGG + Chr4:40080234-40080253 MsG0480020375.01.T01:CDS 40.0%
!! TGAGCATAGGCTTTTGAGAA+AGG + Chr4:40079978-40079997 MsG0480020375.01.T01:CDS 40.0%
ACATAGTGTGATGCTTTGCC+TGG - Chr4:40080925-40080944 None:intergenic 45.0%
ATATGGGTGTGATTCCAACC+AGG + Chr4:40080904-40080923 MsG0480020375.01.T01:CDS 45.0%
ATCAACTCGGAAAGGATCCT+TGG - Chr4:40080275-40080294 None:intergenic 45.0%
GGAAAGGGAAAAGAGAGAAC+GGG - Chr4:40079744-40079763 None:intergenic 45.0%
GGAAGACATGATTACATGGG+TGG + Chr4:40081884-40081903 MsG0480020375.01.T01:CDS 45.0%
GGGAAAGGGAAAAGAGAGAA+CGG - Chr4:40079745-40079764 None:intergenic 45.0%
GGTATACACGGTGAAACAGA+TGG + Chr4:40080246-40080265 MsG0480020375.01.T01:CDS 45.0%
GTATTCGAGTATATGCCGGA+TGG + Chr4:40080213-40080232 MsG0480020375.01.T01:CDS 45.0%
GTTCACTATCATGCAGACGT+AGG + Chr4:40081730-40081749 MsG0480020375.01.T01:intron 45.0%
TCAACTCGGAAAGGATCCTT+GGG - Chr4:40080274-40080293 None:intergenic 45.0%
TTGGAATGGAAGAGAGAAGG+GGG + Chr4:40080009-40080028 MsG0480020375.01.T01:CDS 45.0%
TTGTGGAATTGGGATTGTGG+TGG + Chr4:40080178-40080197 MsG0480020375.01.T01:intron 45.0%
! AAGGGGCAGGCTAGTTAAAT+AGG + Chr4:40081064-40081083 MsG0480020375.01.T01:intron 45.0%
! ACGTAGGCTCTCAACTGTTT+TGG + Chr4:40081746-40081765 MsG0480020375.01.T01:CDS 45.0%
! CTTTTTGTAATCGTCCCGAC+CGG - Chr4:40080073-40080092 None:intergenic 45.0%
! GTGTTCCTTTTACCTGCAGA+AGG - Chr4:40080967-40080986 None:intergenic 45.0%
!! AATAAATTTAATAGAAAAAT+AGG + Chr4:40081434-40081453 MsG0480020375.01.T01:intron 5.0%
AGTGTGATGCTTTGCCTGGT+TGG - Chr4:40080921-40080940 None:intergenic 50.0%
ATGCAGCGCTACCATGTTAC+CGG + Chr4:40080051-40080070 MsG0480020375.01.T01:CDS 50.0%
ATGCCGGATGGTTCAGAAGT+TGG + Chr4:40080225-40080244 MsG0480020375.01.T01:CDS 50.0%
GAACAGGGACATGTTCGAGT+TGG + Chr4:40081816-40081835 MsG0480020375.01.T01:CDS 50.0%
GGTGAAACAGATGGATCCCA+AGG + Chr4:40080255-40080274 MsG0480020375.01.T01:CDS 50.0%
GTCAGCCTTCTGCAGGTAAA+AGG + Chr4:40080959-40080978 MsG0480020375.01.T01:intron 50.0%
TTACCTGCAGAAGGCTGACA+CGG - Chr4:40080958-40080977 None:intergenic 50.0%
! GCGACGGCCAACTAAGTTTT+GGG + Chr4:40081546-40081565 MsG0480020375.01.T01:intron 50.0%
! TGCGACGGCCAACTAAGTTT+TGG + Chr4:40081545-40081564 MsG0480020375.01.T01:intron 50.0%
AATCGTCCCGACCGGTAACA+TGG - Chr4:40080065-40080084 None:intergenic 55.0%
AGAACGGGAACGCACCTTAG+AGG - Chr4:40079729-40079748 None:intergenic 55.0%
AGCGCTACCATGTTACCGGT+CGG + Chr4:40080055-40080074 MsG0480020375.01.T01:CDS 55.0%
AGGGGGTCACAGAGAGAACA+TGG + Chr4:40080026-40080045 MsG0480020375.01.T01:CDS 55.0%
GAACGGGAACGCACCTTAGA+GGG - Chr4:40079728-40079747 None:intergenic 55.0%
TAACCGTGTCAGCCTTCTGC+AGG + Chr4:40080952-40080971 MsG0480020375.01.T01:CDS 55.0%
AACGCACCTTAGAGGGAGGG+AGG - Chr4:40079721-40079740 None:intergenic 60.0%
ACCTTAGAGGGAGGGAGGGA+GGG - Chr4:40079716-40079735 None:intergenic 60.0%
ACGCACCTTAGAGGGAGGGA+GGG - Chr4:40079720-40079739 None:intergenic 60.0%
AGGGAGGGAGGGAAGACAGT+GGG - Chr4:40079705-40079724 None:intergenic 60.0%
GAAGACAGTGGGCCGGTAGA+CGG - Chr4:40079694-40079713 None:intergenic 60.0%
GCGCTACCATGTTACCGGTC+GGG + Chr4:40080056-40080075 MsG0480020375.01.T01:CDS 60.0%
GGGAACGCACCTTAGAGGGA+GGG - Chr4:40079724-40079743 None:intergenic 60.0%
AGGGAGGGAAGACAGTGGGC+CGG - Chr4:40079701-40079720 None:intergenic 65.0%
CACCTTAGAGGGAGGGAGGG+AGG - Chr4:40079717-40079736 None:intergenic 65.0%
CGGGAACGCACCTTAGAGGG+AGG - Chr4:40079725-40079744 None:intergenic 65.0%
GAGGGAGGGAGGGAAGACAG+TGG - Chr4:40079706-40079725 None:intergenic 65.0%
TCCCTCCCTCCCTCCCTCTA+AGG + Chr4:40079712-40079731 MsG0480020375.01.T01:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 40079680 40082174 40079680 ID=MsG0480020375.01;Name=MsG0480020375.01
Chr4 mRNA 40079680 40082174 40079680 ID=MsG0480020375.01.T01;Parent=MsG0480020375.01;Name=MsG0480020375.01.T01;_AED=0.49;_eAED=0.49;_QI=63|1|1|1|0.5|0.4|5|197|182
Chr4 exon 40079680 40079733 40079680 ID=MsG0480020375.01.T01:exon:16790;Parent=MsG0480020375.01.T01
Chr4 exon 40079949 40080091 40079949 ID=MsG0480020375.01.T01:exon:16791;Parent=MsG0480020375.01.T01
Chr4 exon 40080213 40080320 40080213 ID=MsG0480020375.01.T01:exon:16792;Parent=MsG0480020375.01.T01
Chr4 exon 40080899 40080973 40080899 ID=MsG0480020375.01.T01:exon:16793;Parent=MsG0480020375.01.T01
Chr4 exon 40081746 40082174 40081746 ID=MsG0480020375.01.T01:exon:16794;Parent=MsG0480020375.01.T01
Chr4 five_prime_UTR 40079680 40079733 40079680 ID=MsG0480020375.01.T01:five_prime_utr;Parent=MsG0480020375.01.T01
Chr4 five_prime_UTR 40079949 40079957 40079949 ID=MsG0480020375.01.T01:five_prime_utr;Parent=MsG0480020375.01.T01
Chr4 CDS 40079958 40080091 40079958 ID=MsG0480020375.01.T01:cds;Parent=MsG0480020375.01.T01
Chr4 CDS 40080213 40080320 40080213 ID=MsG0480020375.01.T01:cds;Parent=MsG0480020375.01.T01
Chr4 CDS 40080899 40080973 40080899 ID=MsG0480020375.01.T01:cds;Parent=MsG0480020375.01.T01
Chr4 CDS 40081746 40081977 40081746 ID=MsG0480020375.01.T01:cds;Parent=MsG0480020375.01.T01
Chr4 three_prime_UTR 40081978 40082174 40081978 ID=MsG0480020375.01.T01:three_prime_utr;Parent=MsG0480020375.01.T01
Gene Sequence

>MsG0480020375.01.T01

ATGAGGAAGCTAAAGCATCATGAGCATAGGCTTTTGAGAAAGGTGAATTTTTTGGAATGGAAGAGAGAAGGGGGTCACAGAGAGAACATGGTTATGCAGCGCTACCATGTTACCGGTCGGGACGATTACAAAAAGTATTCGAGTATATGCCGGATGGTTCAGAAGTTGGTATACACGGTGAAACAGATGGATCCCAAGGATCCTTTCCGAGTTGATATGACTGATAAGCTTTTGGAAAAACTTTACAATATGGGTGTGATTCCAACCAGGCAAAGCATCACACTATGTGAACGCTTAACCGTGTCAGCCTTCTGCAGACGTAGGCTCTCAACTGTTTTGGTGAAACTAAAATTTGCTGAGCATTTGAAAGAAGCTGTAACTTATATTGAACAGGGACATGTTCGAGTTGGTCCAAATACAATTACAGACCCAGCTTACCTTGTAACTAGAAACATGGAAGACATGATTACATGGGTGGATTCTTCCAAGATAAAGAGAAAGGTGCTTCAGTACAATGACAAGTTGGATGACTATGATTTAATGAATTAG
Protein sequence

>MsG0480020375.01.T01

MRKLKHHEHRLLRKVNFLEWKREGGHRENMVMQRYHVTGRDDYKKYSSICRMVQKLVYTVKQMDPKDPFRVDMTDKLLEKLYNMGVIPTRQSITLCERLTVSAFCRRRLSTVLVKLKFAEHLKEAVTYIEQGHVRVGPNTITDPAYLVTRNMEDMITWVDSSKIKRKVLQYNDKLDDYDLMN*