AlfalfaGEDB | MsG0480021288.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021288.01

KEGG: K14565
GO: -
PFAM: PF01798 PF08156

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021288.01.T01	XP_024637950.1	87.365	554	45	4	1	530	1	553	0	806

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021288.01.T01	O04658	70.721	444	106	3	1	420	1	444	0	627

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021288.01.T01	G7JIJ7	87.365	554	45	4	1	530	1	553	0.0	806

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001967.01	MsG0480021288.01	0.817954	2.521907e-52	1.862728e-49
MsG0180003258.01	MsG0480021288.01	0.804152	2.477800e-49	1.268215e-46
MsG0180003585.01	MsG0480021288.01	0.834301	3.244522e-56	3.834659e-53
MsG0180005384.01	MsG0480021288.01	0.840482	8.466682e-58	1.207634e-54
MsG0180005789.01	MsG0480021288.01	0.803642	3.162680e-49	1.597658e-46
MsG0180005819.01	MsG0480021288.01	0.814619	1.404711e-51	9.471613e-49
MsG0280006301.01	MsG0480021288.01	0.828179	1.038308e-54	1.024306e-51
MsG0280010994.01	MsG0480021288.01	-0.805776	1.133808e-49	6.051031e-47
MsG0480020911.01	MsG0480021288.01	0.835000	2.164673e-56	2.612546e-53
MsG0480021279.01	MsG0480021288.01	0.811876	5.621647e-51	3.520322e-48
MsG0480021288.01	MsG0480021417.01	0.816535	5.260487e-52	3.737258e-49
MsG0480021288.01	MsG0480022006.01	0.844637	6.682248e-59	1.085542e-55
MsG0480021288.01	MsG0480023774.01	0.832255	1.048985e-55	1.166013e-52
MsG0480021288.01	MsG0480023962.01	0.804851	1.771388e-49	9.231733e-47
MsG0480021288.01	MsG0580024738.01	0.802219	6.224967e-49	3.032236e-46
MsG0480021288.01	MsG0580026749.01	0.829053	6.384567e-55	6.460108e-52
MsG0480021288.01	MsG0580028107.01	0.850587	1.542486e-60	3.036111e-57
MsG0480021288.01	MsG0580029047.01	0.829070	6.321028e-55	6.399560e-52
MsG0480021288.01	MsG0680032459.01	0.812900	3.358984e-51	2.161842e-48
MsG0480021288.01	MsG0680034485.01	0.808352	3.230734e-50	1.843156e-47
MsG0480021288.01	MsG0780036015.01	0.837919	3.911364e-57	5.156521e-54
MsG0480021288.01	MsG0780038383.01	0.811666	6.246485e-51	3.889649e-48
MsG0480021288.01	MsG0780038861.01	0.838165	3.381786e-57	4.491612e-54
MsG0480021288.01	MsG0780040174.01	0.892949	9.901693e-75	9.244456e-71
MsG0480021288.01	MsG0780040756.01	0.839151	1.880951e-57	2.574539e-54
MsG0480021288.01	MsG0780040823.01	0.849383	3.350171e-60	6.339827e-57
MsG0480021288.01	MsG0780041522.01	0.839939	1.173415e-57	1.645828e-54
MsG0480021288.01	MsG0780041790.01	0.841092	5.860185e-58	8.517352e-55
MsG0480021288.01	MsG0880042896.01	0.807035	6.153255e-50	3.391515e-47
MsG0480021288.01	MsG0880043076.01	0.809497	1.837727e-50	1.080663e-47
MsG0480021288.01	MsG0880046243.01	0.802539	5.348635e-49	2.626744e-46
MsG0480021288.01	MsG0880046760.01	0.801739	7.811816e-49	3.758922e-46
MsG0480021288.01	MsG0880047310.01	0.817226	3.679871e-52	2.664292e-49
MsG0380015857.01	MsG0480021288.01	0.837140	6.197170e-57	7.976354e-54
MsG0380016760.01	MsG0480021288.01	0.893663	5.097471e-75	4.903083e-71
MsG0380017516.01	MsG0480021288.01	0.880950	3.597756e-70	2.068235e-66
MsG0280006514.01	MsG0480021288.01	0.801933	7.127291e-49	3.446594e-46
MsG0280006717.01	MsG0480021288.01	0.825516	4.492052e-54	4.103456e-51
MsG0280007055.01	MsG0480021288.01	0.809946	1.471292e-50	8.755267e-48
MsG0280007606.01	MsG0480021288.01	0.853771	1.917310e-61	4.188729e-58
MsG0280010519.01	MsG0480021288.01	0.853768	1.920513e-61	4.195321e-58

PPI

Gene1	Gene2	Type
MsG0480021288.01	MsG0280009230.01	PPI
MsG0480021288.01	MsG0480021904.01	PPI
MsG0280009716.01	MsG0480021288.01	PPI
MsG0480021288.01	MsG0880047286.01	PPI
MsG0480021288.01	MsG0880046760.01	PPI
MsG0480021288.01	MsG0180003567.01	PPI
MsG0480021288.01	MsG0280010778.01	PPI
MsG0480021288.01	MsG0380013894.01	PPI
MsG0480021288.01	MsG0180005007.01	PPI
MsG0480021288.01	MsG0280009716.01	PPI
MsG0480021288.01	MsG0380018056.01	PPI
MsG0480021288.01	MsG0180003567.01	PPI
MsG0480021288.01	MsG0480022712.01	PPI
MsG0480021288.01	MsG0580025624.01	PPI
MsG0480021288.01	MsG0480020134.01	PPI
MsG0480021288.01	MsG0480023388.01	PPI
MsG0480021288.01	MsG0780041139.01	PPI
MsG0480021288.01	MsG0280010383.01	PPI
MsG0480021288.01	MsG0480022041.01	PPI
MsG0480021288.01	MsG0280007445.01	PPI
MsG0480021288.01	MsG0280007690.01	PPI
MsG0480021288.01	MsG0480019165.01	PPI
MsG0480021288.01	MsG0480023805.01	PPI
MsG0480021288.01	MsG0180004915.01	PPI
MsG0480021288.01	MsG0280007578.01	PPI
MsG0480021288.01	MsG0380012250.01	PPI
MsG0480021288.01	MsG0180003073.01	PPI
MsG0480021288.01	MsG0880046243.01	PPI
MsG0480021288.01	MsG0380013896.01	PPI
MsG0480021288.01	MsG0480022791.01	PPI
MsG0480021288.01	MsG0880046328.01	PPI
MsG0480021288.01	MsG0180002677.01	PPI
MsG0480021288.01	MsG0680035883.01	PPI
MsG0480021288.01	MsG0880047659.01	PPI
MsG0480021288.01	MsG0180006082.01	PPI
MsG0480021288.01	MsG0180003097.01	PPI
MsG0480021288.01	MsG0480021174.01	PPI
MsG0480021288.01	MsG0880046177.01	PPI
MsG0480021288.01	MsG0780039623.01	PPI
MsG0480021288.01	MsG0780041305.01	PPI
MsG0480021288.01	MsG0380013883.01	PPI
MsG0180003573.01	MsG0480021288.01	PPI
MsG0480021288.01	MsG0680030624.01	PPI
MsG0480021288.01	MsG0480021424.01	PPI
MsG0480021288.01	MsG0280009166.01	PPI
MsG0480021288.01	MsG0280007563.01	PPI
MsG0480021288.01	MsG0380014248.01	PPI
MsG0480021288.01	MsG0480020375.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021288.01.T01	MTR_4g070910	87.365	554	45	4	1	530	1	553	0.0	806
MsG0480021288.01.T01	MTR_5g010260	70.588	578	108	7	1	517	1	577	0.0	723
MsG0480021288.01.T01	MTR_4g103790	42.063	378	184	9	1	345	3	378	1.68e-83	269
MsG0480021288.01.T01	MTR_5g010290	71.014	138	38	1	26	161	9	146	9.74e-59	192
MsG0480021288.01.T01	MTR_7g037560	26.154	195	126	1	159	335	34	228	2.29e-13	72.4
MsG0480021288.01.T01	MTR_7g037560	26.154	195	126	1	159	335	95	289	3.42e-13	72.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021288.01.T01	AT5G27120	70.721	444	106	3	1	420	1	444	0.0	627
MsG0480021288.01.T01	AT3G05060	69.526	443	111	3	1	419	2	444	0.0	620
MsG0480021288.01.T01	AT5G27140	53.991	426	136	6	1	401	1	391	4.69e-138	407
MsG0480021288.01.T01	AT5G27140	49.293	495	155	9	1	470	1	424	1.24e-136	404
MsG0480021288.01.T01	AT1G56110	42.593	378	176	9	1	342	3	375	4.45e-87	278
MsG0480021288.01.T01	AT3G12860	41.779	371	179	7	1	337	3	370	2.34e-84	271

Find 137 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTCACCAAACCCTAATTT+AGG	0.123858	4:+56355303	MsG0480021288.01.T01:five_prime_UTR
ATCAGGTACAAATTCGAATT+TGG	0.128688	4:-56355350	None:intergenic
TGAAGCTTAAACCCTAAATT+AGG	0.186773	4:-56355315	None:intergenic
ATGAATCCTTATTTATTTAA+TGG	0.209120	4:+56359798	MsG0480021288.01.T01:three_prime_UTR
CTTATCCCATTAAATAAATA+AGG	0.229729	4:-56359804	None:intergenic
AAAGAAAGAAAAGAAGAAAA+AGG	0.230780	4:+56359412	MsG0480021288.01.T01:CDS
TTATGTTTGTGAATGGAAAT+AGG	0.241181	4:+56359879	MsG0480021288.01.T01:three_prime_UTR
GGCTATCAGGTGTGATGCAC+TGG	0.262961	4:+56358953	MsG0480021288.01.T01:CDS
TGACTTCAGTGGATAATATA+TGG	0.263866	4:+56359839	MsG0480021288.01.T01:three_prime_UTR
CAAAGACTGCTTTGGCTATC+AGG	0.264189	4:+56358940	MsG0480021288.01.T01:CDS
GAATATTATTAAGGAGAAAT+TGG	0.271601	4:+56356819	MsG0480021288.01.T01:CDS
TGAATCCTTATTTATTTAAT+GGG	0.290361	4:+56359799	MsG0480021288.01.T01:three_prime_UTR
AAAGCTTGGGAATATTATTA+AGG	0.301937	4:+56356810	MsG0480021288.01.T01:CDS
TCAGGTACAAATTCGAATTT+GGG	0.306320	4:-56355349	None:intergenic
TTCTTAACAACTTCCTCTTC+TGG	0.306909	4:-56359473	None:intergenic
TTTCTCCTCCATTCGAAGTA+TGG	0.312200	4:-56359553	None:intergenic
AAATTCGAATTTGGGGATTT+CGG	0.320394	4:-56355341	None:intergenic
CTTCACCAAACCCTAATTTA+GGG	0.323684	4:+56355304	MsG0480021288.01.T01:five_prime_UTR
TTGTCATATGCTTCGATCTT+GGG	0.331868	4:-56359162	None:intergenic
GGTAGTACCGTGCAGATTCT+TGG	0.334079	4:+56358787	MsG0480021288.01.T01:CDS
ACTTGACAGATCTTGCATAT+TGG	0.338912	4:-56358248	None:intergenic
CTTGTCATATGCTTCGATCT+TGG	0.340268	4:-56359163	None:intergenic
GATGTTGTCAACTATGATTT+TGG	0.347858	4:-56358226	None:intergenic
GCTAAACTTCAGCTTGTATC+TGG	0.349651	4:-56357061	None:intergenic
GAAGCTTAAACCCTAAATTA+GGG	0.350432	4:-56355314	None:intergenic
CGCTTTGCCGGTTCTGCTAA+AGG	0.358145	4:+56359135	MsG0480021288.01.T01:CDS
ACTATGATCGTTCAGGCTAT+TGG	0.363794	4:+56358120	MsG0480021288.01.T01:CDS
TTCATGTTAATGCAATTTGT+AGG	0.366624	4:+56359772	MsG0480021288.01.T01:three_prime_UTR
TTAAACCCTAAATTAGGGTT+TGG	0.367394	4:-56355309	None:intergenic
ATATCAATGGGAACTGAAAT+TGG	0.371359	4:+56358569	MsG0480021288.01.T01:CDS
CGTGAAAAGGTGGTTGAATC+AGG	0.379236	4:-56355367	None:intergenic
TAATTAATTATGTTTGTGAA+TGG	0.379604	4:+56359872	MsG0480021288.01.T01:three_prime_UTR
GGCCTAGTTCTTTGCCTTCA+AGG	0.381274	4:-56359113	None:intergenic
ATTTACCATGCATCCTTGAT+TGG	0.385846	4:+56358871	MsG0480021288.01.T01:CDS
GTCAGAGGAGGTTGAGGCAG+AGG	0.395543	4:+56358529	MsG0480021288.01.T01:CDS
CCCCAACCATAGCAGTCAAA+TGG	0.409129	4:-56358702	None:intergenic
CCCATTTGACTGCTATGGTT+GGG	0.410656	4:+56358701	MsG0480021288.01.T01:CDS
CCATGCATCCTTGATTGGTC+AGG	0.410857	4:+56358876	MsG0480021288.01.T01:CDS
GCAGCTACTTTGCTAATTGA+TGG	0.411124	4:+56356715	MsG0480021288.01.T01:CDS
TGTTCATAACAATGCTGTTA+TGG	0.413565	4:+56356939	MsG0480021288.01.T01:CDS
AAAGAACTAGGCCGCTTTGC+CGG	0.414173	4:+56359123	MsG0480021288.01.T01:CDS
GCTATGGTTGGGGAGCTTGT+TGG	0.416805	4:+56358712	MsG0480021288.01.T01:CDS
TGAGACAAACCCAAGCTCAT+AGG	0.424290	4:-56357030	None:intergenic
TTGTCAGTAACAGGAGGCTC+TGG	0.426836	4:-56359371	None:intergenic
TATTAGAGAGCTCTGTGATC+AGG	0.429227	4:+56358610	MsG0480021288.01.T01:CDS
GGGCTTCCCTTTAGCAGAAC+CGG	0.437955	4:-56359142	None:intergenic
GCAACACTCTTCTCAACTTA+AGG	0.438844	4:+56355392	MsG0480021288.01.T01:five_prime_UTR
AAGCATGCTACTCCAAAGTA+TGG	0.441688	4:+56358844	MsG0480021288.01.T01:CDS
CTTTGGTTCAATAACTGAAT+CGG	0.444467	4:-56359321	None:intergenic
AGCTAATAATATACCTTAGC+TGG	0.444788	4:-56359219	None:intergenic
CACTGCAGATGCTGCTAGAA+AGG	0.446366	4:+56356475	MsG0480021288.01.T01:CDS
TCCATTGCAGAATTAGACTT+TGG	0.447306	4:-56359338	None:intergenic
TGCAGCTTGAAGCACGTTTG+AGG	0.448590	4:+56359088	MsG0480021288.01.T01:CDS
GGTGGATACTATGATCGTTC+AGG	0.449376	4:+56358113	MsG0480021288.01.T01:intron
GACATGGCCCCTATGAGCTT+GGG	0.453354	4:+56357021	MsG0480021288.01.T01:CDS
CCATTTGACTGCTATGGTTG+GGG	0.455564	4:+56358702	MsG0480021288.01.T01:CDS
ACTTGCTGCAAAGACTGCTT+TGG	0.456044	4:+56358932	MsG0480021288.01.T01:CDS
AGACATGGCCCCTATGAGCT+TGG	0.460296	4:+56357020	MsG0480021288.01.T01:CDS
GAGGAAGAAGCAGGCAGAGG+AGG	0.461221	4:+56359586	MsG0480021288.01.T01:CDS
AAGTTCAAGGGAAAATTTCT+CGG	0.464901	4:+56358907	MsG0480021288.01.T01:CDS
GCTTTGCCGGTTCTGCTAAA+GGG	0.467457	4:+56359136	MsG0480021288.01.T01:CDS
GAGACAAACCCAAGCTCATA+GGG	0.467946	4:-56357029	None:intergenic
AAAGAAGGAATCTACTGAAA+AGG	0.469310	4:+56359511	MsG0480021288.01.T01:CDS
TATGACAAGGATAGAAAGAA+AGG	0.477667	4:+56359177	MsG0480021288.01.T01:CDS
ACCCATTTGACTGCTATGGT+TGG	0.492132	4:+56358700	MsG0480021288.01.T01:CDS
AGCTGGAGTTATCAGTCCTC+CGG	0.492874	4:-56359202	None:intergenic
GGAGGTTGAGGCAGAGGTGA+AGG	0.493001	4:+56358535	MsG0480021288.01.T01:CDS
ATATGTATCAGCTAATCATA+TGG	0.495601	4:+56359683	MsG0480021288.01.T01:exon
GGTGTTGCTGAGTCAAAGCT+TGG	0.496488	4:+56356796	MsG0480021288.01.T01:CDS
ATATATTATCCACTGAAGTC+AGG	0.498050	4:-56359837	None:intergenic
TGCAAGATCTGTCAAGTTGA+TGG	0.502281	4:+56358254	MsG0480021288.01.T01:CDS
ATTTATTAAGCTTCCTCCAT+GGG	0.503415	4:-56358749	None:intergenic
AGTAGCTGCCTCTAGAGCTT+CGG	0.504643	4:-56356701	None:intergenic
TGGAGCGGCCTGACCAATCA+AGG	0.509639	4:-56358884	None:intergenic
GAAGCTTCAGTGATATCAAT+GGG	0.510808	4:+56358557	MsG0480021288.01.T01:CDS
CACTTTGAAAATAGCAAACC+CGG	0.515199	4:-56355993	None:intergenic
TACTGTTTGAAACACCGGCC+GGG	0.515816	4:+56355975	MsG0480021288.01.T01:CDS
CCCAACCATAGCAGTCAAAT+GGG	0.517666	4:-56358701	None:intergenic
TTGCACCCATTTGACTGCTA+TGG	0.521354	4:+56358696	MsG0480021288.01.T01:CDS
CATTGTGAAAATGAAACGTT+AGG	0.525745	4:+56356775	MsG0480021288.01.T01:CDS
GGAAGCTTCAGTGATATCAA+TGG	0.526035	4:+56358556	MsG0480021288.01.T01:CDS
AATTGATGGCAAAGCTAGCA+AGG	0.527868	4:+56356729	MsG0480021288.01.T01:CDS
GATTTATTAAGCTTCCTCCA+TGG	0.528986	4:-56358750	None:intergenic
AAAGAGGAAGAAGCAGGCAG+AGG	0.529182	4:+56359583	MsG0480021288.01.T01:CDS
ATGCCATGAGGGTTCGTGAA+TGG	0.538629	4:+56358178	MsG0480021288.01.T01:CDS
GCACGTTTGAGGAACCTTGA+AGG	0.539843	4:+56359099	MsG0480021288.01.T01:CDS
ATAAATCTTGCAAAGCAGCC+TGG	0.545223	4:+56358766	MsG0480021288.01.T01:CDS
TCAGCTAATCATATGGTGAC+TGG	0.547032	4:+56359690	MsG0480021288.01.T01:exon
AACCTTGAAGGCAAAGAACT+AGG	0.547172	4:+56359111	MsG0480021288.01.T01:CDS
GCTTCTACTGTTTGAAACAC+CGG	0.548474	4:+56355970	MsG0480021288.01.T01:CDS
AAGAGTGTTGCGGCGTGAAA+AGG	0.550147	4:-56355380	None:intergenic
TCAGGCCGCTCCAAAGTTCA+AGG	0.553933	4:+56358894	MsG0480021288.01.T01:CDS
AGGAAAGCTTTCTAAAGTTG+AGG	0.554316	4:+56356024	MsG0480021288.01.T01:CDS
GTGTTGCTGAGTCAAAGCTT+GGG	0.558313	4:+56356797	MsG0480021288.01.T01:CDS
AAGGATAGAAAGAAAGGAGC+CGG	0.559519	4:+56359183	MsG0480021288.01.T01:CDS
CTACTGTTTGAAACACCGGC+CGG	0.563221	4:+56355974	MsG0480021288.01.T01:CDS
AGAATCTGCACGGTACTACC+AGG	0.563552	4:-56358784	None:intergenic
AATGGAGACCATACTTCGAA+TGG	0.570280	4:+56359545	MsG0480021288.01.T01:CDS
GAAAAGGCAGAGGTGCAGAA+TGG	0.571720	4:+56359527	MsG0480021288.01.T01:CDS
GGTGCTCGCCTCATTGCCCA+TGG	0.577178	4:+56358733	MsG0480021288.01.T01:CDS
CAATTGAATGAACTCATAGC+AGG	0.584388	4:+56356985	MsG0480021288.01.T01:CDS
TAGCAGTCAAATGGGTGCAA+TGG	0.587721	4:-56358693	None:intergenic
TTCGAATTTGGGGATTTCGG+TGG	0.588460	4:-56355338	None:intergenic
ACCAAAGTCTAATTCTGCAA+TGG	0.590650	4:+56359337	MsG0480021288.01.T01:CDS
GCTATCAGGTGTGATGCACT+GGG	0.592574	4:+56358954	MsG0480021288.01.T01:CDS
GAATACTTCCGAAGCTCTAG+AGG	0.594176	4:+56356693	MsG0480021288.01.T01:CDS
AGCTTCCTCCATGGGCAATG+AGG	0.595189	4:-56358741	None:intergenic
TGGTAAATGAGACCATACTT+TGG	0.597333	4:-56358856	None:intergenic
TGTTGTTGAGGAACCAGAAG+AGG	0.598846	4:+56359460	MsG0480021288.01.T01:CDS
ATGAGGGTTCGTGAATGGTA+TGG	0.601117	4:+56358183	MsG0480021288.01.T01:CDS
ATACCATTCACGAACCCTCA+TGG	0.603195	4:-56358181	None:intergenic
GGAATCTACTGAAAAGGCAG+AGG	0.607137	4:+56359517	MsG0480021288.01.T01:CDS
GCTGAAGTTTAGCGCTGACA+AGG	0.610350	4:+56357071	MsG0480021288.01.T01:CDS
GCAAGATCTGTCAAGTTGAT+GGG	0.615767	4:+56358255	MsG0480021288.01.T01:CDS
TTGAAAATAGCAAACCCGGC+CGG	0.617400	4:-56355989	None:intergenic
GCTGAACACGTATGCCATGA+GGG	0.618620	4:+56358167	MsG0480021288.01.T01:CDS
CCTGACCAATCAAGGATGCA+TGG	0.619510	4:-56358876	None:intergenic
AGGACTGATAACTCCAGCTA+AGG	0.620619	4:+56359206	MsG0480021288.01.T01:CDS
CAGGTACAAATTCGAATTTG+GGG	0.627757	4:-56355348	None:intergenic
CTCGGCACCAAGAATCTGCA+CGG	0.631244	4:-56358794	None:intergenic
AGTGTTGCGGCGTGAAAAGG+TGG	0.634974	4:-56355377	None:intergenic
CAGGCCGCTCCAAAGTTCAA+GGG	0.637067	4:+56358895	MsG0480021288.01.T01:CDS
AGGTCTAGCTGTTCAAGACA+TGG	0.637643	4:+56357005	MsG0480021288.01.T01:CDS
CAAGATCGAAGCATATGACA+AGG	0.641584	4:+56359164	MsG0480021288.01.T01:CDS
TTAAGTTGAGAAGAGTGTTG+CGG	0.642630	4:-56355390	None:intergenic
AGCTGAACACGTATGCCATG+AGG	0.646581	4:+56358166	MsG0480021288.01.T01:CDS
CTTTCTAGCAGCATCTGCAG+TGG	0.650736	4:-56356474	None:intergenic
GGAGACCATACTTCGAATGG+AGG	0.650803	4:+56359548	MsG0480021288.01.T01:CDS
ATTGATGGCAAAGCTAGCAA+GGG	0.654528	4:+56356730	MsG0480021288.01.T01:CDS
GGGTTCGTGAATGGTATGGT+TGG	0.660131	4:+56358187	MsG0480021288.01.T01:CDS
GCTCGCCTCATTGCCCATGG+AGG	0.662199	4:+56358736	MsG0480021288.01.T01:CDS
GATAGAAAGAAAGGAGCCGG+AGG	0.682277	4:+56359186	MsG0480021288.01.T01:CDS
TGCAAAGCTTGATTTCTCTG+AGG	0.684043	4:+56358293	MsG0480021288.01.T01:CDS
GGTGGTTGAAGATGTTGTTG+AGG	0.689600	4:+56359448	MsG0480021288.01.T01:CDS
AGACAAACCCAAGCTCATAG+GGG	0.697188	4:-56357028	None:intergenic
CTTTAGCAGAACCGGCAAAG+CGG	0.708275	4:-56359134	None:intergenic
GCTGTTATGGAGTTGATGAG+AGG	0.738756	4:+56356952	MsG0480021288.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACACTAAATAATCAAATTT+AGG	+	Chr4:56356639-56356658	MsG0480021288.01.T01:intron	15.0%
!!	TAATTAATTATGTTTGTGAA+TGG	+	Chr4:56359872-56359891	MsG0480021288.01.T01:three_prime_UTR	15.0%
!!!	ATGAATCCTTATTTATTTAA+TGG	+	Chr4:56359798-56359817	MsG0480021288.01.T01:three_prime_UTR	15.0%
!!!	TATCTATATCTATTTGTTAT+TGG	-	Chr4:56358377-56358396	None:intergenic	15.0%
!!!	TCAAATAGCTAATTTTTTAT+CGG	+	Chr4:56356894-56356913	MsG0480021288.01.T01:intron	15.0%
!!!	TGAATCCTTATTTATTTAAT+GGG	+	Chr4:56359799-56359818	MsG0480021288.01.T01:three_prime_UTR	15.0%
!!!	TTTTCAAATTCAAAATGTAT+AGG	+	Chr4:56356319-56356338	MsG0480021288.01.T01:intron	15.0%
!!!	TTTTCAGGTTTATTAATTTA+AGG	+	Chr4:56355938-56355957	MsG0480021288.01.T01:intron	15.0%
!!	AAAGAAAGAAAAGAAGAAAA+AGG	+	Chr4:56359412-56359431	MsG0480021288.01.T01:CDS	20.0%
!!	AACTCTAAATATATATATCC+AGG	-	Chr4:56357664-56357683	None:intergenic	20.0%
!!	ACAACAAAATTGGAAATTTA+AGG	-	Chr4:56357978-56357997	None:intergenic	20.0%
!!	ACTAAATAATCAAATTTAGG+TGG	+	Chr4:56356642-56356661	MsG0480021288.01.T01:intron	20.0%
!!	AGAAACCTAAAAAACTAAAT+TGG	-	Chr4:56355504-56355523	None:intergenic	20.0%
!!	CTTATCCCATTAAATAAATA+AGG	-	Chr4:56359807-56359826	None:intergenic	20.0%
!!	GAATATTATTAAGGAGAAAT+TGG	+	Chr4:56356819-56356838	MsG0480021288.01.T01:CDS	20.0%
!!	TATGCAATGAGTAAATTAAT+TGG	-	Chr4:56358347-56358366	None:intergenic	20.0%
!!	TCTTGAAATGAAAGAAAAAA+TGG	-	Chr4:56356295-56356314	None:intergenic	20.0%
!!	TGGTAAAATATAAATTGGTT+AGG	-	Chr4:56356421-56356440	None:intergenic	20.0%
!!	TTAACTAAAAATCAACTGTT+TGG	+	Chr4:56357408-56357427	MsG0480021288.01.T01:intron	20.0%
!!!	ACTTTATGATGCTTTTTATT+AGG	+	Chr4:56358092-56358111	MsG0480021288.01.T01:intron	20.0%
!!!	ATCATAAAGACAGTTTTATT+CGG	+	Chr4:56357771-56357790	MsG0480021288.01.T01:intron	20.0%
!!!	ATTTTGTTTAAGAGTAACTT+AGG	-	Chr4:56356852-56356871	None:intergenic	20.0%
!!!	GAAGTTGGTAAAATATAAAT+TGG	-	Chr4:56356426-56356445	None:intergenic	20.0%
!!!	GATTTTATCATTTTTTAACC+TGG	+	Chr4:56357643-56357662	MsG0480021288.01.T01:intron	20.0%
!!!	GGTGTTATTTAATTTTCTAT+TGG	+	Chr4:56359248-56359267	MsG0480021288.01.T01:intron	20.0%
!!!	TATATCATTTTGTTCATCAT+CGG	-	Chr4:56355624-56355643	None:intergenic	20.0%
!!!	TTGTTCCAATTTAGTTTTTT+AGG	+	Chr4:56355496-56355515	MsG0480021288.01.T01:intron	20.0%
!!!	TTTATTGCTTTGTTTTTTTC+AGG	+	Chr4:56355923-56355942	MsG0480021288.01.T01:intron	20.0%
!	AAAGAAGAAAAAGGAGAAAA+AGG	+	Chr4:56359421-56359440	MsG0480021288.01.T01:CDS	25.0%
!	AAAGCTTGGGAATATTATTA+AGG	+	Chr4:56356810-56356829	MsG0480021288.01.T01:CDS	25.0%
!	AAGACAAAGTATAAATACAG+TGG	-	Chr4:56356395-56356414	None:intergenic	25.0%
!	AGAAAAGAAGAAGAAAAAGA+AGG	+	Chr4:56359496-56359515	MsG0480021288.01.T01:CDS	25.0%
!	ATATGTATCAGCTAATCATA+TGG	+	Chr4:56359683-56359702	MsG0480021288.01.T01:exon	25.0%
!	CAAACAAATCACAACAAAAT+TGG	-	Chr4:56357988-56358007	None:intergenic	25.0%
!	CTGTATTTATACTTTGTCTT+CGG	+	Chr4:56356395-56356414	MsG0480021288.01.T01:intron	25.0%
!	GATACACATAATTAAAGAGT+TGG	-	Chr4:56357754-56357773	None:intergenic	25.0%
!	TAAATCCTGTATAAAGAAGT+TGG	-	Chr4:56356441-56356460	None:intergenic	25.0%
!	TAAGTCTTAAGCTACTATTT+CGG	-	Chr4:56357871-56357890	None:intergenic	25.0%
!	TAGCAAAAAATGTACGATTT+AGG	-	Chr4:56355532-56355551	None:intergenic	25.0%
!	TCATGCAATCTTAAGAATAA+AGG	-	Chr4:56357299-56357318	None:intergenic	25.0%
!	TGACAAAAAGAAAGAAAAGA+AGG	+	Chr4:56359388-56359407	MsG0480021288.01.T01:CDS	25.0%
!	TGCATTATTTCACTTTGATA+AGG	-	Chr4:56357576-56357595	None:intergenic	25.0%
!	TTAACTAAAAATCGACTGTT+CGG	+	Chr4:56357355-56357374	MsG0480021288.01.T01:intron	25.0%
!	TTATGTTTGTGAATGGAAAT+AGG	+	Chr4:56359879-56359898	MsG0480021288.01.T01:three_prime_UTR	25.0%
!	TTCATGTTAATGCAATTTGT+AGG	+	Chr4:56359772-56359791	MsG0480021288.01.T01:three_prime_UTR	25.0%
!	TTGAAAACATAGTACAACAA+TGG	-	Chr4:56357144-56357163	None:intergenic	25.0%
!!	ATTGTGTGTTTTATAGGTTA+TGG	+	Chr4:56355570-56355589	MsG0480021288.01.T01:intron	25.0%
!!	CAAAATCTGAAATTCATTTC+AGG	-	Chr4:56358510-56358529	None:intergenic	25.0%
!!	CTGAATCTTTTAACATTTCT+AGG	+	Chr4:56357916-56357935	MsG0480021288.01.T01:intron	25.0%
!!	CTTTTTATCTTTCTTTTGCT+CGG	-	Chr4:56359632-56359651	None:intergenic	25.0%
!!	GGTATATTATTAGCTTCATT+TGG	+	Chr4:56359227-56359246	MsG0480021288.01.T01:intron	25.0%
!!	TTGTGTGTTTTATAGGTTAT+GGG	+	Chr4:56355571-56355590	MsG0480021288.01.T01:intron	25.0%
!!	TTTTACCAACTTCTTTATAC+AGG	+	Chr4:56356433-56356452	MsG0480021288.01.T01:intron	25.0%
!!!	ATAAAAAGCATCATAAAGTC+AGG	-	Chr4:56358091-56358110	None:intergenic	25.0%
!!!	TAACCATTTTTGTATACTTC+TGG	+	Chr4:56359713-56359732	MsG0480021288.01.T01:three_prime_UTR	25.0%
!!!	TCATAAAGACAGTTTTATTC+GGG	+	Chr4:56357772-56357791	MsG0480021288.01.T01:intron	25.0%
!!!	TTATGATGCTTTTTATTAGG+TGG	+	Chr4:56358095-56358114	MsG0480021288.01.T01:intron	25.0%
!!!	TTTGTTGTGATTTGTTTGTA+TGG	+	Chr4:56357990-56358009	MsG0480021288.01.T01:intron	25.0%
	AAAGAAGGAATCTACTGAAA+AGG	+	Chr4:56359511-56359530	MsG0480021288.01.T01:CDS	30.0%
	AAAGAAGTTAGGCACAAATA+TGG	+	Chr4:56357234-56357253	MsG0480021288.01.T01:intron	30.0%
	AAATTCGAATTTGGGGATTT+CGG	-	Chr4:56355344-56355363	None:intergenic	30.0%
	AACATGAAATTGAGAGGATA+AGG	-	Chr4:56356525-56356544	None:intergenic	30.0%
	AAGTTCAAGGGAAAATTTCT+CGG	+	Chr4:56358907-56358926	MsG0480021288.01.T01:CDS	30.0%
	ACATGAAATTGAGAGGATAA+GGG	-	Chr4:56356524-56356543	None:intergenic	30.0%
	AGCTAATAATATACCTTAGC+TGG	-	Chr4:56359222-56359241	None:intergenic	30.0%
	ATATATTATCCACTGAAGTC+AGG	-	Chr4:56359840-56359859	None:intergenic	30.0%
	ATATCAATGGGAACTGAAAT+TGG	+	Chr4:56358569-56358588	MsG0480021288.01.T01:CDS	30.0%
	ATCAGGTACAAATTCGAATT+TGG	-	Chr4:56355353-56355372	None:intergenic	30.0%
	ATTTATTAAGCTTCCTCCAT+GGG	-	Chr4:56358752-56358771	None:intergenic	30.0%
	CATTGTGAAAATGAAACGTT+AGG	+	Chr4:56356775-56356794	MsG0480021288.01.T01:CDS	30.0%
	CTTACAATTCAATGTTCTTG+AGG	+	Chr4:56357887-56357906	MsG0480021288.01.T01:intron	30.0%
	GAAGCTTAAACCCTAAATTA+GGG	-	Chr4:56355317-56355336	None:intergenic	30.0%
	GTGACTTCAATATATTGATC+AGG	-	Chr4:56357167-56357186	None:intergenic	30.0%
	TATGACAAGGATAGAAAGAA+AGG	+	Chr4:56359177-56359196	MsG0480021288.01.T01:CDS	30.0%
	TATTTCGGTTACCATTTACT+CGG	-	Chr4:56357856-56357875	None:intergenic	30.0%
	TCAGGTACAAATTCGAATTT+GGG	-	Chr4:56355352-56355371	None:intergenic	30.0%
	TCTATGATTATCTCAAGAGT+AGG	+	Chr4:56358663-56358682	MsG0480021288.01.T01:intron	30.0%
	TGAAGCTTAAACCCTAAATT+AGG	-	Chr4:56355318-56355337	None:intergenic	30.0%
	TGACTTCAGTGGATAATATA+TGG	+	Chr4:56359839-56359858	MsG0480021288.01.T01:three_prime_UTR	30.0%
	TGTTCATAACAATGCTGTTA+TGG	+	Chr4:56356939-56356958	MsG0480021288.01.T01:CDS	30.0%
	TTAAACCCTAAATTAGGGTT+TGG	-	Chr4:56355312-56355331	None:intergenic	30.0%
	TTCTCTCAGCAATATGATAA+TGG	+	Chr4:56356548-56356567	MsG0480021288.01.T01:intron	30.0%
	TTTCTAGGCAAATATGCATA+TGG	+	Chr4:56357931-56357950	MsG0480021288.01.T01:intron	30.0%
!	AGAAATTTTCCCTTGAACTT+TGG	-	Chr4:56358907-56358926	None:intergenic	30.0%
!	AGGGTTATTGTGTGTTTTAT+AGG	+	Chr4:56355564-56355583	MsG0480021288.01.T01:intron	30.0%
!	ATACATATTTTCGCTCTAGT+CGG	-	Chr4:56359671-56359690	None:intergenic	30.0%
!	CTTTGGTTCAATAACTGAAT+CGG	-	Chr4:56359324-56359343	None:intergenic	30.0%
!	GATGTTGTCAACTATGATTT+TGG	-	Chr4:56358229-56358248	None:intergenic	30.0%
!	TCTTTCTTTTTGTCAGTAAC+AGG	-	Chr4:56359383-56359402	None:intergenic	30.0%
!	TGAATTTCAGATTTTGTCAG+AGG	+	Chr4:56358514-56358533	MsG0480021288.01.T01:intron	30.0%
!	TGTTTTATAGGTTATGGGTT+TGG	+	Chr4:56355576-56355595	MsG0480021288.01.T01:intron	30.0%
!!	ACTATGATTTTGGTAAGTTC+TGG	-	Chr4:56358219-56358238	None:intergenic	30.0%
!!	ATTTTCAAAGTGCTTGATGA+AGG	+	Chr4:56356004-56356023	MsG0480021288.01.T01:CDS	30.0%
!!	TTTGCTACTGATTTGTTAGA+GGG	+	Chr4:56355545-56355564	MsG0480021288.01.T01:intron	30.0%
!!!	TCTAAACCTTCTGGTTTTAA+TGG	-	Chr4:56357551-56357570	None:intergenic	30.0%
!!!	TTTTGCTACTGATTTGTTAG+AGG	+	Chr4:56355544-56355563	MsG0480021288.01.T01:intron	30.0%
	AAAGTGAAATAATGCAGCGA+GGG	+	Chr4:56357580-56357599	MsG0480021288.01.T01:intron	35.0%
	ACCAAAGTCTAATTCTGCAA+TGG	+	Chr4:56359337-56359356	MsG0480021288.01.T01:CDS	35.0%
	ACTTGACAGATCTTGCATAT+TGG	-	Chr4:56358251-56358270	None:intergenic	35.0%
	ATGACTAGCAAAGCAGAATA+TGG	-	Chr4:56357213-56357232	None:intergenic	35.0%
	ATGTATAGGAGTTCTCAATC+CGG	+	Chr4:56356333-56356352	MsG0480021288.01.T01:intron	35.0%
	ATTTACCATGCATCCTTGAT+TGG	+	Chr4:56358871-56358890	MsG0480021288.01.T01:CDS	35.0%
	ATTTCGGTTACCATTTACTC+GGG	-	Chr4:56357855-56357874	None:intergenic	35.0%
	CAATTGAATGAACTCATAGC+AGG	+	Chr4:56356985-56357004	MsG0480021288.01.T01:CDS	35.0%
	CACCATTAGAACGTTAGTTA+AGG	-	Chr4:56357799-56357818	None:intergenic	35.0%
	CACTGTGTTTAATACCACTA+AGG	+	Chr4:56356232-56356251	MsG0480021288.01.T01:intron	35.0%
	CAGGTACAAATTCGAATTTG+GGG	-	Chr4:56355351-56355370	None:intergenic	35.0%
	CTTAACTAACGTTCTAATGG+TGG	+	Chr4:56357797-56357816	MsG0480021288.01.T01:intron	35.0%
	CTTAGTGGTATTAAACACAG+TGG	-	Chr4:56356234-56356253	None:intergenic	35.0%
	CTTCACCAAACCCTAATTTA+GGG	+	Chr4:56355304-56355323	MsG0480021288.01.T01:five_prime_UTR	35.0%
	GAAAAAGGAGAAAAAGGAAG+AGG	+	Chr4:56359427-56359446	MsG0480021288.01.T01:CDS	35.0%
	GAAGCAAACATGAAATTGAG+AGG	-	Chr4:56356531-56356550	None:intergenic	35.0%
	GAAGCTTCAGTGATATCAAT+GGG	+	Chr4:56358557-56358576	MsG0480021288.01.T01:CDS	35.0%
	GACCTTAACTAACGTTCTAA+TGG	+	Chr4:56357794-56357813	MsG0480021288.01.T01:intron	35.0%
	GAGGAGAAAAGAAGAAAAAG+AGG	+	Chr4:56359567-56359586	MsG0480021288.01.T01:CDS	35.0%
	GATTTATTAAGCTTCCTCCA+TGG	-	Chr4:56358753-56358772	None:intergenic	35.0%
	GTAATTGTGAGAGAACAAAC+AGG	+	Chr4:56357430-56357449	MsG0480021288.01.T01:intron	35.0%
	TCCATTGCAGAATTAGACTT+TGG	-	Chr4:56359341-56359360	None:intergenic	35.0%
	TCTGTGCGGAAGAAAATTAT+TGG	+	Chr4:56355666-56355685	MsG0480021288.01.T01:intron	35.0%
	TCTTCACCAAACCCTAATTT+AGG	+	Chr4:56355303-56355322	MsG0480021288.01.T01:five_prime_UTR	35.0%
	TGAGTAAATTAATTGGCAGC+AGG	-	Chr4:56358340-56358359	None:intergenic	35.0%
	TGCTAGTCATCAAAGAAGTT+AGG	+	Chr4:56357223-56357242	MsG0480021288.01.T01:intron	35.0%
	TGGTAAATGAGACCATACTT+TGG	-	Chr4:56358859-56358878	None:intergenic	35.0%
	TTCTTAACAACTTCCTCTTC+TGG	-	Chr4:56359476-56359495	None:intergenic	35.0%
	TTGTCATATGCTTCGATCTT+GGG	-	Chr4:56359165-56359184	None:intergenic	35.0%
!	ATTTCAGATTTTGTCAGAGG+AGG	+	Chr4:56358517-56358536	MsG0480021288.01.T01:intron	35.0%
!	CACTTTGAAAATAGCAAACC+CGG	-	Chr4:56355996-56356015	None:intergenic	35.0%
!	GCTCCAGAAGTATACAAAAA+TGG	-	Chr4:56359719-56359738	None:intergenic	35.0%
!	TTCTTTTTGTCAGTAACAGG+AGG	-	Chr4:56359380-56359399	None:intergenic	35.0%
!	TTTATCTTTCTTTTGCTCGG+CGG	-	Chr4:56359629-56359648	None:intergenic	35.0%
!	TTTTTCACCGAACAGTTTTG+TGG	+	Chr4:56357499-56357518	MsG0480021288.01.T01:intron	35.0%
!!	AGGAAAGCTTTCTAAAGTTG+AGG	+	Chr4:56356024-56356043	MsG0480021288.01.T01:CDS	35.0%
!!	GTTGTGATTTGTTTGTATGG+TGG	+	Chr4:56357993-56358012	MsG0480021288.01.T01:intron	35.0%
!!	TGCTCTAAAAAGAGCTTTCT+CGG	-	Chr4:56358815-56358834	None:intergenic	35.0%
!!	TTAAGTTGAGAAGAGTGTTG+CGG	-	Chr4:56355393-56355412	None:intergenic	35.0%
!!	TTTGGTGTTTGCTTGTAACT+GGG	+	Chr4:56355821-56355840	MsG0480021288.01.T01:intron	35.0%
!!	TTTGTTTTTCCTGACTTCAG+TGG	+	Chr4:56359828-56359847	MsG0480021288.01.T01:three_prime_UTR	35.0%
!!!	TTGGCAAAGTCAACGTTTTT+TGG	+	Chr4:56355803-56355822	MsG0480021288.01.T01:intron	35.0%
!!!	TTTTGGTGTTTGCTTGTAAC+TGG	+	Chr4:56355820-56355839	MsG0480021288.01.T01:intron	35.0%
	AAAGGAGAAAAAGGAAGAGG+TGG	+	Chr4:56359430-56359449	MsG0480021288.01.T01:CDS	40.0%
	AAATTGAGAGGATAAGGGTG+TGG	-	Chr4:56356519-56356538	None:intergenic	40.0%
	AACCTTGAAGGCAAAGAACT+AGG	+	Chr4:56359111-56359130	MsG0480021288.01.T01:CDS	40.0%
	AAGAGTTGGACCTAACACTA+CGG	-	Chr4:56357740-56357759	None:intergenic	40.0%
	AAGCATGCTACTCCAAAGTA+TGG	+	Chr4:56358844-56358863	MsG0480021288.01.T01:CDS	40.0%
	AAGGATAGAAAGAAAGGAGC+CGG	+	Chr4:56359183-56359202	MsG0480021288.01.T01:CDS	40.0%
	AATGGAGACCATACTTCGAA+TGG	+	Chr4:56359545-56359564	MsG0480021288.01.T01:CDS	40.0%
	ACCCAGAAAACAGACACAAT+GGG	-	Chr4:56355782-56355801	None:intergenic	40.0%
	ACTATGATCGTTCAGGCTAT+TGG	+	Chr4:56358120-56358139	MsG0480021288.01.T01:CDS	40.0%
	AGGAGACCATTAAAACCAGA+AGG	+	Chr4:56357542-56357561	MsG0480021288.01.T01:intron	40.0%
	AGTCTTCTTTGTATCACAGC+TGG	-	Chr4:56357711-56357730	None:intergenic	40.0%
	ATAAATCTTGCAAAGCAGCC+TGG	+	Chr4:56358766-56358785	MsG0480021288.01.T01:CDS	40.0%
	ATTATTGGAGACCTGAACTG+AGG	+	Chr4:56355681-56355700	MsG0480021288.01.T01:intron	40.0%
	CAAAGTGAAATAATGCAGCG+AGG	+	Chr4:56357579-56357598	MsG0480021288.01.T01:intron	40.0%
	CAAGATCGAAGCATATGACA+AGG	+	Chr4:56359164-56359183	MsG0480021288.01.T01:CDS	40.0%
	CAGCGCAAGAGATAGTAAAT+AGG	-	Chr4:56355455-56355474	None:intergenic	40.0%
	CTAACGTTCTAATGGTGGTA+AGG	+	Chr4:56357802-56357821	MsG0480021288.01.T01:intron	40.0%
	CTTGTCATATGCTTCGATCT+TGG	-	Chr4:56359166-56359185	None:intergenic	40.0%
	GAAGAAAAAGAGGAAGAAGC+AGG	+	Chr4:56359577-56359596	MsG0480021288.01.T01:CDS	40.0%
	GCAACACTCTTCTCAACTTA+AGG	+	Chr4:56355392-56355411	MsG0480021288.01.T01:five_prime_UTR	40.0%
	GCTAAACTTCAGCTTGTATC+TGG	-	Chr4:56357064-56357083	None:intergenic	40.0%
	GGAAGCTTCAGTGATATCAA+TGG	+	Chr4:56358556-56358575	MsG0480021288.01.T01:CDS	40.0%
	TAGATTCTGCACTTCCTTAG+TGG	-	Chr4:56356249-56356268	None:intergenic	40.0%
	TATTAGAGAGCTCTGTGATC+AGG	+	Chr4:56358610-56358629	MsG0480021288.01.T01:CDS	40.0%
	TCAGCTAATCATATGGTGAC+TGG	+	Chr4:56359690-56359709	MsG0480021288.01.T01:exon	40.0%
	TGCAAAGCTTGATTTCTCTG+AGG	+	Chr4:56358293-56358312	MsG0480021288.01.T01:CDS	40.0%
	TTCTAATGTGCCCGAGTAAA+TGG	+	Chr4:56357842-56357861	MsG0480021288.01.T01:intron	40.0%
	TTTCTCCTCCATTCGAAGTA+TGG	-	Chr4:56359556-56359575	None:intergenic	40.0%
!	AATTGAGAGGATAAGGGTGT+GGG	-	Chr4:56356518-56356537	None:intergenic	40.0%
!	AATTGATGGCAAAGCTAGCA+AGG	+	Chr4:56356729-56356748	MsG0480021288.01.T01:CDS	40.0%
!	ATTGATGGCAAAGCTAGCAA+GGG	+	Chr4:56356730-56356749	MsG0480021288.01.T01:CDS	40.0%
!	CTTTTTCTTCTTCAGCTAGC+TGG	+	Chr4:56358041-56358060	MsG0480021288.01.T01:intron	40.0%
!	GATAAGGCATCTAAACCTTC+TGG	-	Chr4:56357560-56357579	None:intergenic	40.0%
!	GCAGCTACTTTGCTAATTGA+TGG	+	Chr4:56356715-56356734	MsG0480021288.01.T01:CDS	40.0%
!	GCTTCTACTGTTTGAAACAC+CGG	+	Chr4:56355970-56355989	MsG0480021288.01.T01:CDS	40.0%
!	TCCCATTGTGTCTGTTTTCT+GGG	+	Chr4:56355778-56355797	MsG0480021288.01.T01:intron	40.0%
!	TTCCCATTGTGTCTGTTTTC+TGG	+	Chr4:56355777-56355796	MsG0480021288.01.T01:intron	40.0%
!!	GCAAGATCTGTCAAGTTGAT+GGG	+	Chr4:56358255-56358274	MsG0480021288.01.T01:CDS	40.0%
!!	TCTTCTGAGATGTTTCTGTG+CGG	+	Chr4:56355652-56355671	MsG0480021288.01.T01:intron	40.0%
!!	TGCAAGATCTGTCAAGTTGA+TGG	+	Chr4:56358254-56358273	MsG0480021288.01.T01:CDS	40.0%
!!	TTTTCCCTTGAACTTTGGAG+CGG	-	Chr4:56358902-56358921	None:intergenic	40.0%
	AATCAATACCAGAGCTCCAC+CGG	-	Chr4:56357456-56357475	None:intergenic	45.0%
	ACCCATTTGACTGCTATGGT+TGG	+	Chr4:56358700-56358719	MsG0480021288.01.T01:CDS	45.0%
	AGACAAACCCAAGCTCATAG+GGG	-	Chr4:56357031-56357050	None:intergenic	45.0%
	AGAGTTGGACCTAACACTAC+GGG	-	Chr4:56357739-56357758	None:intergenic	45.0%
	AGGACTGATAACTCCAGCTA+AGG	+	Chr4:56359206-56359225	MsG0480021288.01.T01:CDS	45.0%
	AGGTCTAGCTGTTCAAGACA+TGG	+	Chr4:56357005-56357024	MsG0480021288.01.T01:CDS	45.0%
	ATACCATTCACGAACCCTCA+TGG	-	Chr4:56358184-56358203	None:intergenic	45.0%
	ATGAGGGTTCGTGAATGGTA+TGG	+	Chr4:56358183-56358202	MsG0480021288.01.T01:CDS	45.0%
	CAAAGACTGCTTTGGCTATC+AGG	+	Chr4:56358940-56358959	MsG0480021288.01.T01:CDS	45.0%
	CACCCAGAAAACAGACACAA+TGG	-	Chr4:56355783-56355802	None:intergenic	45.0%
	CCATTTGACTGCTATGGTTG+GGG	+	Chr4:56358702-56358721	MsG0480021288.01.T01:CDS	45.0%
	CCCAACCATAGCAGTCAAAT+GGG	-	Chr4:56358704-56358723	None:intergenic	45.0%
	CCCATTTGACTGCTATGGTT+GGG	+	Chr4:56358701-56358720	MsG0480021288.01.T01:CDS	45.0%
	CGTGAAAAGGTGGTTGAATC+AGG	-	Chr4:56355370-56355389	None:intergenic	45.0%
	GAAATAATGCAGCGAGGGAT+AGG	+	Chr4:56357585-56357604	MsG0480021288.01.T01:intron	45.0%
	GACAGTACATAACGCTTCAC+CGG	-	Chr4:56356355-56356374	None:intergenic	45.0%
	GACTATATCCCCGTAGTGTT+AGG	+	Chr4:56357727-56357746	MsG0480021288.01.T01:intron	45.0%
	GAGACAAACCCAAGCTCATA+GGG	-	Chr4:56357032-56357051	None:intergenic	45.0%
	GCAAGATAACACCATGGGAT+TGG	+	Chr4:56358983-56359002	MsG0480021288.01.T01:intron	45.0%
	GGAATCTACTGAAAAGGCAG+AGG	+	Chr4:56359517-56359536	MsG0480021288.01.T01:CDS	45.0%
	GGGATTGGAAAACAGAGCTA+AGG	+	Chr4:56358998-56359017	MsG0480021288.01.T01:intron	45.0%
	GGTGGATACTATGATCGTTC+AGG	+	Chr4:56358113-56358132	MsG0480021288.01.T01:intron	45.0%
	GGTGGTTGAAGATGTTGTTG+AGG	+	Chr4:56359448-56359467	MsG0480021288.01.T01:CDS	45.0%
	TAGCAGTCAAATGGGTGCAA+TGG	-	Chr4:56358696-56358715	None:intergenic	45.0%
	TGAAAGAGTCTCCTCAGTTC+AGG	-	Chr4:56355695-56355714	None:intergenic	45.0%
	TGAGACAAACCCAAGCTCAT+AGG	-	Chr4:56357033-56357052	None:intergenic	45.0%
	TGTTGTTGAGGAACCAGAAG+AGG	+	Chr4:56359460-56359479	MsG0480021288.01.T01:CDS	45.0%
	TTCGAATTTGGGGATTTCGG+TGG	-	Chr4:56355341-56355360	None:intergenic	45.0%
	TTGAAAATAGCAAACCCGGC+CGG	-	Chr4:56355992-56356011	None:intergenic	45.0%
	TTGCACCCATTTGACTGCTA+TGG	+	Chr4:56358696-56358715	MsG0480021288.01.T01:CDS	45.0%
	TTGTGAGAGAACAAACAGGC+CGG	+	Chr4:56357434-56357453	MsG0480021288.01.T01:intron	45.0%
!	ACTTGCTGCAAAGACTGCTT+TGG	+	Chr4:56358932-56358951	MsG0480021288.01.T01:CDS	45.0%
!	AGCTCTGTTTTCCAATCCCA+TGG	-	Chr4:56358997-56359016	None:intergenic	45.0%
!	GAATACTTCCGAAGCTCTAG+AGG	+	Chr4:56356693-56356712	MsG0480021288.01.T01:CDS	45.0%
!	GATTTTGTCAGAGGAGGTTG+AGG	+	Chr4:56358523-56358542	MsG0480021288.01.T01:intron	45.0%
!	GTGTTGCTGAGTCAAAGCTT+GGG	+	Chr4:56356797-56356816	MsG0480021288.01.T01:CDS	45.0%
!	TGTGTCTGTTTTCTGGGTGT+TGG	+	Chr4:56355784-56355803	MsG0480021288.01.T01:intron	45.0%
!!	GCTGTTATGGAGTTGATGAG+AGG	+	Chr4:56356952-56356971	MsG0480021288.01.T01:CDS	45.0%
!!	TTCACCGAACAGTTTTGTGG+CGG	+	Chr4:56357502-56357521	MsG0480021288.01.T01:intron	45.0%
	AAAGAGGAAGAAGCAGGCAG+AGG	+	Chr4:56359583-56359602	MsG0480021288.01.T01:CDS	50.0%
	AGAATCTGCACGGTACTACC+AGG	-	Chr4:56358787-56358806	None:intergenic	50.0%
	AGCGAGGGATAGGAGAATAG+AGG	+	Chr4:56357595-56357614	MsG0480021288.01.T01:intron	50.0%
	AGCTGAACACGTATGCCATG+AGG	+	Chr4:56358166-56358185	MsG0480021288.01.T01:CDS	50.0%
	AGCTGGAGTTATCAGTCCTC+CGG	-	Chr4:56359205-56359224	None:intergenic	50.0%
	ATGCCATGAGGGTTCGTGAA+TGG	+	Chr4:56358178-56358197	MsG0480021288.01.T01:CDS	50.0%
	CACTGCAGATGCTGCTAGAA+AGG	+	Chr4:56356475-56356494	MsG0480021288.01.T01:CDS	50.0%
	CCCCAACCATAGCAGTCAAA+TGG	-	Chr4:56358705-56358724	None:intergenic	50.0%
	CCTGACCAATCAAGGATGCA+TGG	-	Chr4:56358879-56358898	None:intergenic	50.0%
	CTTTAGCAGAACCGGCAAAG+CGG	-	Chr4:56359137-56359156	None:intergenic	50.0%
	GACGGGCAAGATAACACCAT+GGG	+	Chr4:56358978-56358997	MsG0480021288.01.T01:intron	50.0%
	GAGTTGGACCTAACACTACG+GGG	-	Chr4:56357738-56357757	None:intergenic	50.0%
	GATAGAAAGAAAGGAGCCGG+AGG	+	Chr4:56359186-56359205	MsG0480021288.01.T01:CDS	50.0%
	GCACGTTTGAGGAACCTTGA+AGG	+	Chr4:56359099-56359118	MsG0480021288.01.T01:CDS	50.0%
	GCGAGGGATAGGAGAATAGA+GGG	+	Chr4:56357596-56357615	MsG0480021288.01.T01:intron	50.0%
	GCTATCAGGTGTGATGCACT+GGG	+	Chr4:56358954-56358973	MsG0480021288.01.T01:CDS	50.0%
	GCTGAACACGTATGCCATGA+GGG	+	Chr4:56358167-56358186	MsG0480021288.01.T01:CDS	50.0%
	GGAGACCATACTTCGAATGG+AGG	+	Chr4:56359548-56359567	MsG0480021288.01.T01:CDS	50.0%
	GGGTTCGTGAATGGTATGGT+TGG	+	Chr4:56358187-56358206	MsG0480021288.01.T01:CDS	50.0%
	GGTAGTACCGTGCAGATTCT+TGG	+	Chr4:56358787-56358806	MsG0480021288.01.T01:CDS	50.0%
	TGCACCGCCACAAAACTGTT+CGG	-	Chr4:56357509-56357528	None:intergenic	50.0%
	TGCAGCTTGAAGCACGTTTG+AGG	+	Chr4:56359088-56359107	MsG0480021288.01.T01:CDS	50.0%
	TTGTCAGTAACAGGAGGCTC+TGG	-	Chr4:56359374-56359393	None:intergenic	50.0%
!	AAAGAACTAGGCCGCTTTGC+CGG	+	Chr4:56359123-56359142	MsG0480021288.01.T01:CDS	50.0%
!	CCATGCATCCTTGATTGGTC+AGG	+	Chr4:56358876-56358895	MsG0480021288.01.T01:CDS	50.0%
!	CGGTGCAGGAGTATGAGTTT+AGG	+	Chr4:56357522-56357541	MsG0480021288.01.T01:intron	50.0%
!	CTACTGTTTGAAACACCGGC+CGG	+	Chr4:56355974-56355993	MsG0480021288.01.T01:CDS	50.0%
!	CTTTCTAGCAGCATCTGCAG+TGG	-	Chr4:56356477-56356496	None:intergenic	50.0%
!	GAAAAGGCAGAGGTGCAGAA+TGG	+	Chr4:56359527-56359546	MsG0480021288.01.T01:CDS	50.0%
!	GGCCTAGTTCTTTGCCTTCA+AGG	-	Chr4:56359116-56359135	None:intergenic	50.0%
!	TACTGTTTGAAACACCGGCC+GGG	+	Chr4:56355975-56355994	MsG0480021288.01.T01:CDS	50.0%
!	TGACGGGCAAGATAACACCA+TGG	+	Chr4:56358977-56358996	MsG0480021288.01.T01:intron	50.0%
!!	AAGAGTGTTGCGGCGTGAAA+AGG	-	Chr4:56355383-56355402	None:intergenic	50.0%
!!	AGTAGCTGCCTCTAGAGCTT+CGG	-	Chr4:56356704-56356723	None:intergenic	50.0%
!!	GCTGAAGTTTAGCGCTGACA+AGG	+	Chr4:56357071-56357090	MsG0480021288.01.T01:CDS	50.0%
!!	GCTTTGCCGGTTCTGCTAAA+GGG	+	Chr4:56359136-56359155	MsG0480021288.01.T01:CDS	50.0%
!!	GGTGTTGCTGAGTCAAAGCT+TGG	+	Chr4:56356796-56356815	MsG0480021288.01.T01:CDS	50.0%
	AGACATGGCCCCTATGAGCT+TGG	+	Chr4:56357020-56357039	MsG0480021288.01.T01:CDS	55.0%
	AGCTTCCTCCATGGGCAATG+AGG	-	Chr4:56358744-56358763	None:intergenic	55.0%
	AGGTGTGATGCACTGGGTGA+CGG	+	Chr4:56358960-56358979	MsG0480021288.01.T01:intron	55.0%
	AGTGTTGCGGCGTGAAAAGG+TGG	-	Chr4:56355380-56355399	None:intergenic	55.0%
	CAGGCCGCTCCAAAGTTCAA+GGG	+	Chr4:56358895-56358914	MsG0480021288.01.T01:CDS	55.0%
	CGGGCACATTAGAAAGCAGC+TGG	-	Chr4:56357836-56357855	None:intergenic	55.0%
	CTCGGCACCAAGAATCTGCA+CGG	-	Chr4:56358797-56358816	None:intergenic	55.0%
	GACATGGCCCCTATGAGCTT+GGG	+	Chr4:56357021-56357040	MsG0480021288.01.T01:CDS	55.0%
	GCTATGGTTGGGGAGCTTGT+TGG	+	Chr4:56358712-56358731	MsG0480021288.01.T01:CDS	55.0%
	GGCTATCAGGTGTGATGCAC+TGG	+	Chr4:56358953-56358972	MsG0480021288.01.T01:CDS	55.0%
	GGGCTTCCCTTTAGCAGAAC+CGG	-	Chr4:56359145-56359164	None:intergenic	55.0%
	TCAGGCCGCTCCAAAGTTCA+AGG	+	Chr4:56358894-56358913	MsG0480021288.01.T01:CDS	55.0%
	TGAGAGAACAAACAGGCCGG+TGG	+	Chr4:56357437-56357456	MsG0480021288.01.T01:intron	55.0%
!!	CGCTTTGCCGGTTCTGCTAA+AGG	+	Chr4:56359135-56359154	MsG0480021288.01.T01:CDS	55.0%
!!	GAACAGTTTTGTGGCGGTGC+AGG	+	Chr4:56357508-56357527	MsG0480021288.01.T01:intron	55.0%
	GAGGAAGAAGCAGGCAGAGG+AGG	+	Chr4:56359586-56359605	MsG0480021288.01.T01:CDS	60.0%
	GGAGGTTGAGGCAGAGGTGA+AGG	+	Chr4:56358535-56358554	MsG0480021288.01.T01:CDS	60.0%
	GGTGTGATGCACTGGGTGAC+GGG	+	Chr4:56358961-56358980	MsG0480021288.01.T01:intron	60.0%
	GTCAGAGGAGGTTGAGGCAG+AGG	+	Chr4:56358529-56358548	MsG0480021288.01.T01:CDS	60.0%
	TGGAGCGGCCTGACCAATCA+AGG	-	Chr4:56358887-56358906	None:intergenic	60.0%
	CAAACAGGCCGGTGGAGCTC+TGG	+	Chr4:56357445-56357464	MsG0480021288.01.T01:intron	65.0%
	GCTCGCCTCATTGCCCATGG+AGG	+	Chr4:56358736-56358755	MsG0480021288.01.T01:CDS	65.0%
	GGTGCTCGCCTCATTGCCCA+TGG	+	Chr4:56358733-56358752	MsG0480021288.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	56355288	56359907	56355288	ID=MsG0480021288.01;Name=MsG0480021288.01
Chr4	mRNA	56355288	56359907	56355288	ID=MsG0480021288.01.T01;Parent=MsG0480021288.01;Name=MsG0480021288.01.T01;_AED=0.44;_eAED=0.44;_QI=149\|0.77\|0.7\|1\|0.88\|0.8\|10\|210\|539
Chr4	exon	56355288	56355413	56355288	ID=MsG0480021288.01.T01:exon:6909;Parent=MsG0480021288.01.T01
Chr4	exon	56355945	56356045	56355945	ID=MsG0480021288.01.T01:exon:6910;Parent=MsG0480021288.01.T01
Chr4	exon	56356455	56356496	56356455	ID=MsG0480021288.01.T01:exon:6911;Parent=MsG0480021288.01.T01
Chr4	exon	56356661	56356840	56356661	ID=MsG0480021288.01.T01:exon:6912;Parent=MsG0480021288.01.T01
Chr4	exon	56356928	56357092	56356928	ID=MsG0480021288.01.T01:exon:6913;Parent=MsG0480021288.01.T01
Chr4	exon	56358114	56358314	56358114	ID=MsG0480021288.01.T01:exon:6914;Parent=MsG0480021288.01.T01
Chr4	exon	56358524	56358631	56358524	ID=MsG0480021288.01.T01:exon:6915;Parent=MsG0480021288.01.T01
Chr4	exon	56358685	56358975	56358685	ID=MsG0480021288.01.T01:exon:6916;Parent=MsG0480021288.01.T01
Chr4	exon	56359066	56359227	56359066	ID=MsG0480021288.01.T01:exon:6917;Parent=MsG0480021288.01.T01
Chr4	exon	56359305	56359907	56359305	ID=MsG0480021288.01.T01:exon:6918;Parent=MsG0480021288.01.T01
Chr4	five_prime_UTR	56355288	56355413	56355288	ID=MsG0480021288.01.T01:five_prime_utr;Parent=MsG0480021288.01.T01
Chr4	five_prime_UTR	56355945	56355967	56355945	ID=MsG0480021288.01.T01:five_prime_utr;Parent=MsG0480021288.01.T01
Chr4	CDS	56355968	56356045	56355968	ID=MsG0480021288.01.T01:cds;Parent=MsG0480021288.01.T01
Chr4	CDS	56356455	56356496	56356455	ID=MsG0480021288.01.T01:cds;Parent=MsG0480021288.01.T01
Chr4	CDS	56356661	56356840	56356661	ID=MsG0480021288.01.T01:cds;Parent=MsG0480021288.01.T01
Chr4	CDS	56356928	56357092	56356928	ID=MsG0480021288.01.T01:cds;Parent=MsG0480021288.01.T01
Chr4	CDS	56358114	56358314	56358114	ID=MsG0480021288.01.T01:cds;Parent=MsG0480021288.01.T01
Chr4	CDS	56358524	56358631	56358524	ID=MsG0480021288.01.T01:cds;Parent=MsG0480021288.01.T01
Chr4	CDS	56358685	56358975	56358685	ID=MsG0480021288.01.T01:cds;Parent=MsG0480021288.01.T01
Chr4	CDS	56359066	56359227	56359066	ID=MsG0480021288.01.T01:cds;Parent=MsG0480021288.01.T01
Chr4	CDS	56359305	56359697	56359305	ID=MsG0480021288.01.T01:cds;Parent=MsG0480021288.01.T01
Chr4	three_prime_UTR	56359698	56359907	56359698	ID=MsG0480021288.01.T01:three_prime_utr;Parent=MsG0480021288.01.T01

Gene Sequence

>MsG0480021288.01.T01

ATGCTTCTACTGTTTGAAACACCGGCCGGGTTTGCTATTTTCAAAGTGCTTGATGAAGGAAAGCTTTCTAAAGTTGAGGATTTACAGAACGAGTTTACCACTGCAGATGCTGCTAGAAAGGTGGTCAAATTGAAAGCTTTTAACAAATTTGCGAATACTTCCGAAGCTCTAGAGGCAGCTACTTTGCTAATTGATGGCAAAGCTAGCAAGGGTCTGCGCAAGTTTCTACGTGTTCATTGTGAAAATGAAACGTTAGGTGTTGCTGAGTCAAAGCTTGGGAATATTATTAAGGAGAAATTGAAAATCGAATGTGTTCATAACAATGCTGTTATGGAGTTGATGAGAGGTGTCAGATATCAATTGAATGAACTCATAGCAGGTCTAGCTGTTCAAGACATGGCCCCTATGAGCTTGGGTTTGTCTCATAGCTTATCCAGATACAAGCTGAAGTTTAGCGCTGACAAGGTGGATACTATGATCGTTCAGGCTATTGGTTTGCTTGATGATCTTGATAAAGAGCTGAACACGTATGCCATGAGGGTTCGTGAATGGTATGGTTGGCATTTTCCAGAACTTACCAAAATCATAGTTGACAACATCCAATATGCAAGATCTGTCAAGTTGATGGGTGATCGCATTAATGCTGCAAAGCTTGATTTCTCTGAGATTTTGTCAGAGGAGGTTGAGGCAGAGGTGAAGGAAGCTTCAGTGATATCAATGGGAACTGAAATTGGTGAGCTTGATTTGTCAAATATTAGAGAGCTCTGTGATCAGGATGAACACCATTGCACCCATTTGACTGCTATGGTTGGGGAGCTTGTTGGTGCTCGCCTCATTGCCCATGGAGGAAGCTTAATAAATCTTGCAAAGCAGCCTGGTAGTACCGTGCAGATTCTTGGTGCCGAGAAAGCTCTTTTTAGAGCACTGAAGACTAAGCATGCTACTCCAAAGTATGGTCTCATTTACCATGCATCCTTGATTGGTCAGGCCGCTCCAAAGTTCAAGGGAAAATTTCTCGGTCACTTGCTGCAAAGACTGCTTTGGCTATCAGGTGTGATGCACTGGATTTTATTTACACAAGTTTTTGTGCAGCTTGAAGCACGTTTGAGGAACCTTGAAGGCAAAGAACTAGGCCGCTTTGCCGGTTCTGCTAAAGGGAAGCCCAAGATCGAAGCATATGACAAGGATAGAAAGAAAGGAGCCGGAGGACTGATAACTCCAGCTAAGACATATAATACTGCAGCCGATTCAGTTATTGAACCAAAGTCTAATTCTGCAATGGATGAAGATACACCAGAGCCTCCTGTTACTGACAAAAAGAAAGAAAAGAAGGAAAAGAAAGAAAAGAAGAAAAAGGAGAAAAAGGAAGAGGTGGTTGAAGATGTTGTTGAGGAACCAGAAGAGGAAGTTGTTAAGAAAGAAAAGAAGAAGAAAAAGAAGGAATCTACTGAAAAGGCAGAGGTGCAGAATGGAGACCATACTTCGAATGGAGGAGAAAAGAAGAAAAAGAGGAAGAAGCAGGCAGAGGAGGAAGACTCTGCTGAGATGCCGCCGAGCAAAAGAAAGATAAAAAGAAAAAGAAGAAAAGAGCCGACTAGAGCGAAAATATGTATCAGCTAA

Protein sequence

>MsG0480021288.01.T01

MLLLFETPAGFAIFKVLDEGKLSKVEDLQNEFTTADAARKVVKLKAFNKFANTSEALEAATLLIDGKASKGLRKFLRVHCENETLGVAESKLGNIIKEKLKIECVHNNAVMELMRGVRYQLNELIAGLAVQDMAPMSLGLSHSLSRYKLKFSADKVDTMIVQAIGLLDDLDKELNTYAMRVREWYGWHFPELTKIIVDNIQYARSVKLMGDRINAAKLDFSEILSEEVEAEVKEASVISMGTEIGELDLSNIRELCDQDEHHCTHLTAMVGELVGARLIAHGGSLINLAKQPGSTVQILGAEKALFRALKTKHATPKYGLIYHASLIGQAAPKFKGKFLGHLLQRLLWLSGVMHWILFTQVFVQLEARLRNLEGKELGRFAGSAKGKPKIEAYDKDRKKGAGGLITPAKTYNTAADSVIEPKSNSAMDEDTPEPPVTDKKKEKKEKKEKKKKEKKEEVVEDVVEEPEEEVVKKEKKKKKKESTEKAEVQNGDHTSNGGEKKKKRKKQAEEEDSAEMPPSKRKIKRKRRKEPTRAKICIS*