AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020575.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020575.01.T01 MTR_4g054290 92.171 281 22 0 2 282 88 368 0.0 515
MsG0480020575.01.T01 MTR_7g090120 82.206 281 50 0 2 282 88 368 7.77e-162 463
MsG0480020575.01.T01 MTR_4g054150 83.274 281 26 1 2 282 88 347 6.20e-161 461
MsG0480020575.01.T01 MTR_7g090100 82.206 281 50 0 2 282 88 368 9.85e-161 461
MsG0480020575.01.T01 MTR_7g090140 82.918 281 48 0 2 282 88 368 2.48e-160 459
MsG0480020575.01.T01 MTR_7g090140 84.249 273 43 0 10 282 1 273 2.50e-159 454
MsG0480020575.01.T01 MTR_8g009590 63.475 282 102 1 2 282 127 408 1.71e-118 354
MsG0480020575.01.T01 MTR_8g009560 67.260 281 92 0 2 282 91 371 7.11e-118 351
MsG0480020575.01.T01 MTR_4g129630 31.541 279 188 1 7 282 95 373 7.27e-45 160
MsG0480020575.01.T01 MTR_8g009550 61.333 75 26 1 83 157 16 87 1.72e-22 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020575.01.T01 AT1G02205 61.210 281 109 0 2 282 92 372 2.23e-111 335
MsG0480020575.01.T01 AT1G02205 61.210 281 109 0 2 282 91 371 4.47e-111 334
MsG0480020575.01.T01 AT1G02205 61.210 281 109 0 2 282 91 371 5.69e-111 334
MsG0480020575.01.T01 AT2G37700 58.007 281 117 1 2 281 87 367 2.69e-104 313
MsG0480020575.01.T01 AT1G02190 60.071 283 108 4 2 279 91 373 1.46e-102 312
MsG0480020575.01.T01 AT1G02190 60.071 283 108 4 2 279 91 373 1.88e-102 312
MsG0480020575.01.T01 AT2G37700 58.007 281 117 1 2 281 87 367 2.04e-102 311
MsG0480020575.01.T01 AT2G37700 58.007 281 117 1 2 281 87 367 2.17e-102 311
MsG0480020575.01.T01 AT2G37700 50.534 281 101 4 2 281 87 330 3.93e-77 245
MsG0480020575.01.T01 AT1G02205 58.000 200 84 0 83 282 8 207 1.75e-75 238
MsG0480020575.01.T01 AT5G57800 33.451 284 181 3 7 282 93 376 1.14e-45 162
MsG0480020575.01.T01 AT1G02205 54.400 125 57 0 158 282 8 132 3.15e-41 146

Find 72 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTATATATGACTACATTTA+TGG 0.133350 4:-43658565 MsG0480020575.01.T01:CDS
TGACATATTGGTGTTATAAT+TGG 0.202761 4:+43660973 None:intergenic
AAGTAGGAGAAGAGTTTCTT+AGG 0.273284 4:+43659079 None:intergenic
AGCATAGAGCACGTTGAAAA+AGG 0.296954 4:+43658361 None:intergenic
CTAAAATGCGGAAGAATTCT+AGG 0.303873 4:-43660638 MsG0480020575.01.T01:intron
CCAACCCTCAAACATCTAAA+TGG 0.316593 4:-43658402 MsG0480020575.01.T01:CDS
TTACATGTATATCACTTAGA+TGG 0.320308 4:+43660853 None:intergenic
AAAAGTAATAAATGACATAT+TGG 0.369066 4:+43660961 None:intergenic
CTCATCACAAATTCATACTA+TGG 0.373931 4:-43661182 MsG0480020575.01.T01:five_prime_UTR
TGTATGTGAAATACTTCAAT+TGG 0.395814 4:+43659053 None:intergenic
GTTTCCAAGGCCAGTTTGTA+AGG 0.398023 4:+43661143 None:intergenic
CGCTGCATCACACAAAATTT+AGG 0.401705 4:-43658609 MsG0480020575.01.T01:CDS
TTGACACATAAATTAATTTG+TGG 0.403250 4:+43661002 None:intergenic
TGTTTGAGGGTTGGAGGCTA+AGG 0.409276 4:+43658411 None:intergenic
GAAACCTCTTGGGAGCTTCA+AGG 0.410003 4:-43661125 MsG0480020575.01.T01:five_prime_UTR
CCATCTTTCAACTACGCTTA+GGG 0.416116 4:-43658441 MsG0480020575.01.T01:CDS
CCATAAATGTAGTCATATAT+AGG 0.417569 4:+43658565 None:intergenic
TCCATCTTTCAACTACGCTT+AGG 0.422294 4:-43658442 MsG0480020575.01.T01:CDS
TATGCCAACATTTCAGCAAA+TGG 0.424110 4:+43659232 None:intergenic
TGAACAATGTTGTCAACATT+GGG 0.427233 4:+43659191 None:intergenic
GCATCCTTACAAACTGGCCT+TGG 0.438647 4:-43661147 MsG0480020575.01.T01:five_prime_UTR
ATGAACAATGTTGTCAACAT+TGG 0.446199 4:+43659190 None:intergenic
GTTTGTAAGGATGCCCGCTT+TGG 0.446245 4:+43661156 None:intergenic
ACTTTACGCAATATTTCCTA+TGG 0.446705 4:-43659546 MsG0480020575.01.T01:CDS
AACTGGCCTTGGAAACCTCT+TGG 0.451153 4:-43661136 MsG0480020575.01.T01:five_prime_UTR
CATAAATTAATTTGTGGAAA+GGG 0.455417 4:+43661008 None:intergenic
CTTACAGCTCTCTTGCATGC+TGG 0.459101 4:-43659485 MsG0480020575.01.T01:CDS
TGTGTTAAGTGCACCACATC+TGG 0.460275 4:+43658481 None:intergenic
AGGTACCATTTAGATGTTTG+AGG 0.465285 4:+43658397 None:intergenic
CAGTGGAATTCTTGTACTAC+TGG 0.467990 4:-43659460 MsG0480020575.01.T01:CDS
CCCTAAGCGTAGTTGAAAGA+TGG 0.468595 4:+43658441 None:intergenic
AAGCGGGCATCCTTACAAAC+TGG 0.468776 4:-43661153 MsG0480020575.01.T01:five_prime_UTR
ACTGGCCTTGGAAACCTCTT+GGG 0.470484 4:-43661135 MsG0480020575.01.T01:five_prime_UTR
GGTACCATTTAGATGTTTGA+GGG 0.478371 4:+43658398 None:intergenic
CATACTATGGCTTCCAAAGC+GGG 0.482595 4:-43661169 MsG0480020575.01.T01:five_prime_UTR
ACCACATCTGGTGATTCCTC+TGG 0.483089 4:+43658493 None:intergenic
CCATTTAGATGTTTGAGGGT+TGG 0.485770 4:+43658402 None:intergenic
TTTAGATGTTTGAGGGTTGG+AGG 0.486191 4:+43658405 None:intergenic
ATACTTCAATTGGGGAAAGT+AGG 0.487785 4:+43659063 None:intergenic
TGCTTGTGACTTGGATTAGT+GGG 0.489293 4:-43658342 MsG0480020575.01.T01:CDS
CGTGCTCTATGCTTGTGACT+TGG 0.492557 4:-43658351 MsG0480020575.01.T01:CDS
ATGCTTGTGACTTGGATTAG+TGG 0.496658 4:-43658343 MsG0480020575.01.T01:CDS
AAGATCTCAAGCTGCAGTGT+TGG 0.498853 4:-43658288 MsG0480020575.01.T01:CDS
TTAATTTGTGGAAAGGGTCG+AGG 0.500617 4:+43661014 None:intergenic
GAAGTTACTTTCTAAAACAA+TGG 0.502211 4:+43658315 None:intergenic
ACATAAATTAATTTGTGGAA+AGG 0.508491 4:+43661007 None:intergenic
TCGTTTGTGGCTGCACTGTA+TGG 0.516902 4:-43659160 MsG0480020575.01.T01:CDS
GTATGTGAAATACTTCAATT+GGG 0.520031 4:+43659054 None:intergenic
TTGAAGAATGATGGTGAGAA+TGG 0.522722 4:+43659396 None:intergenic
TCTATTACTTTCTTGTTGAG+AGG 0.529467 4:+43660816 None:intergenic
TGTTCTCCACCATTGTAAGT+TGG 0.536824 4:+43659520 None:intergenic
TAATTTGTGGAAAGGGTCGA+GGG 0.541950 4:+43661015 None:intergenic
CTATGGCTGCCAACTTACAA+TGG 0.545118 4:-43659529 MsG0480020575.01.T01:CDS
ATTGTCACTGAGCCTATCAC+TGG 0.557450 4:-43659374 MsG0480020575.01.T01:intron
ACATCCATTTGCTGAAATGT+TGG 0.559199 4:-43659236 MsG0480020575.01.T01:CDS
AGCTTCATATGTAGCATCTG+AGG 0.581755 4:+43658528 None:intergenic
AAGCTCCCAAGAGGTTTCCA+AGG 0.582916 4:+43661130 None:intergenic
CAGTGACAATTGAAGAATGA+TGG 0.584374 4:+43659387 None:intergenic
CATTGTAAGTTGGCAGCCAT+AGG 0.587199 4:+43659530 None:intergenic
CAGTAGTACAAGAATTCCAC+TGG 0.588431 4:+43659461 None:intergenic
AATGGTACCTCAATTTAATG+TGG 0.603244 4:-43658384 MsG0480020575.01.T01:CDS
TCTCTTGCATGCTGGACCAG+TGG 0.604144 4:-43659477 MsG0480020575.01.T01:CDS
TTACAATGGTGGAGAACAGA+TGG 0.615918 4:-43659515 MsG0480020575.01.T01:CDS
ACCAGAGGAATCACCAGATG+TGG 0.626932 4:-43658494 MsG0480020575.01.T01:CDS
TGGCTGCCAACTTACAATGG+TGG 0.632530 4:-43659526 MsG0480020575.01.T01:CDS
TCATACTATGGCTTCCAAAG+CGG 0.640747 4:-43661170 MsG0480020575.01.T01:five_prime_UTR
ACAACCTTGAAGCTCCCAAG+AGG 0.664103 4:+43661121 None:intergenic
AATTTGTGGAAAGGGTCGAG+GGG 0.678754 4:+43661016 None:intergenic
TGACTACATTTATGGTACTG+TGG 0.682689 4:-43658557 MsG0480020575.01.T01:CDS
ATTCAGCGAGACCTAAAATG+CGG 0.707321 4:-43660650 MsG0480020575.01.T01:exon
TATGTGAAATACTTCAATTG+GGG 0.721848 4:+43659055 None:intergenic
AGCTTGTGTGAAGAGACCAG+AGG 0.773132 4:-43658509 MsG0480020575.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTTTATTTTTTTA+TGG - Chr4:43660521-43660540 MsG0480020575.01.T01:intron 0.0%
!! TCTTTAATTGATAAATAAAT+CGG + Chr4:43659697-43659716 None:intergenic 10.0%
!!! TAATTTTTCCAATTATTTTA+GGG - Chr4:43660442-43660461 MsG0480020575.01.T01:intron 10.0%
!!! TTAATTTTTCCAATTATTTT+AGG - Chr4:43660441-43660460 MsG0480020575.01.T01:intron 10.0%
!! AAAAGTAATAAATGACATAT+TGG + Chr4:43658479-43658498 None:intergenic 15.0%
!! AAATGAAATATAAGTAAAAG+TGG - Chr4:43658662-43658681 MsG0480020575.01.T01:intron 15.0%
!! AACAATAAGTTTCTTATATT+AGG + Chr4:43660150-43660169 None:intergenic 15.0%
!! AACTATACTAATCAATTATA+AGG + Chr4:43659011-43659030 None:intergenic 15.0%
!! ACAATAAGTTTCTTATATTA+GGG + Chr4:43660149-43660168 None:intergenic 15.0%
!! CCTATTAATTATAATCAATT+GGG + Chr4:43659572-43659591 None:intergenic 15.0%
!! GATTATAATTAATAGGAATT+AGG - Chr4:43659576-43659595 MsG0480020575.01.T01:CDS 15.0%
!! TAATATAAGAAACTTATTGT+TGG - Chr4:43660149-43660168 MsG0480020575.01.T01:intron 15.0%
!! TCCTATTAATTATAATCAAT+TGG + Chr4:43659573-43659592 None:intergenic 15.0%
!!! AATTTTTCCAATTATTTTAG+GGG - Chr4:43660443-43660462 MsG0480020575.01.T01:intron 15.0%
!!! CTTTTACTTATATTTCATTT+TGG + Chr4:43658662-43658681 None:intergenic 15.0%
!!! TTCTTTATTGCATATTTTTA+TGG - Chr4:43660743-43660762 MsG0480020575.01.T01:intron 15.0%
!!! TTTTTATTAAAGTGAATGTT+AGG - Chr4:43658539-43658558 MsG0480020575.01.T01:CDS 15.0%
!!! TTTTTGCTTATATTTCATTT+CGG - Chr4:43658749-43658768 MsG0480020575.01.T01:intron 15.0%
!!! TTTTTTATGGTGTTTATTTT+CGG - Chr4:43660534-43660553 MsG0480020575.01.T01:intron 15.0%
!! AAAAGTTCCAATACAAATAA+AGG + Chr4:43660102-43660121 None:intergenic 20.0%
!! AAACAGATAATACAAACTAA+AGG + Chr4:43660581-43660600 None:intergenic 20.0%
!! AAAGTTCCAATACAAATAAA+GGG + Chr4:43660101-43660120 None:intergenic 20.0%
!! CCCAATTGATTATAATTAAT+AGG - Chr4:43659569-43659588 MsG0480020575.01.T01:CDS 20.0%
!! GTTCAAAAATCAATAAACAT+TGG - Chr4:43658828-43658847 MsG0480020575.01.T01:intron 20.0%
!! TAAAGCCAAAATTGAAATTT+AGG + Chr4:43659536-43659555 None:intergenic 20.0%
!!! ACATAAATTAATTTGTGGAA+AGG + Chr4:43658433-43658452 None:intergenic 20.0%
!!! ATAGCATATACTCTTTTATA+TGG - Chr4:43659343-43659362 MsG0480020575.01.T01:intron 20.0%
!!! CATAAATTAATTTGTGGAAA+GGG + Chr4:43658432-43658451 None:intergenic 20.0%
!!! TAAATTTCCTAGTTTTTTCT+AGG + Chr4:43658973-43658992 None:intergenic 20.0%
!!! TAATTGTGTATGATTTGTTA+TGG - Chr4:43659652-43659671 MsG0480020575.01.T01:intron 20.0%
!!! TAGCATATACTCTTTTATAT+GGG - Chr4:43659344-43659363 MsG0480020575.01.T01:intron 20.0%
!!! TTACTTATATTTCATTTTGG+AGG + Chr4:43658659-43658678 None:intergenic 20.0%
!!! TTGACACATAAATTAATTTG+TGG + Chr4:43658438-43658457 None:intergenic 20.0%
!!! TTTTTATGGTGTTTATTTTC+GGG - Chr4:43660535-43660554 MsG0480020575.01.T01:intron 20.0%
! AAAAGAAGAATTGTTGACAA+AGG - Chr4:43659471-43659490 MsG0480020575.01.T01:CDS 25.0%
! AACATTCTGACGAATATATA+AGG + Chr4:43659402-43659421 None:intergenic 25.0%
! AAGTAAAAGTGGTAATTGAA+AGG - Chr4:43658673-43658692 MsG0480020575.01.T01:intron 25.0%
! ACATTCTGACGAATATATAA+GGG + Chr4:43659401-43659420 None:intergenic 25.0%
! AGGCTATTCTTGAAAAATTA+TGG + Chr4:43658953-43658972 None:intergenic 25.0%
! AGTTTAAAAGTTGAAGTATG+TGG + Chr4:43658725-43658744 None:intergenic 25.0%
! ATTGAAATTTAGGAACCAAT+TGG + Chr4:43659526-43659545 None:intergenic 25.0%
! CAAAAATCAATAAACATTGG+CGG - Chr4:43658831-43658850 MsG0480020575.01.T01:intron 25.0%
! CCATAAATGTAGTCATATAT+AGG + Chr4:43660875-43660894 None:intergenic 25.0%
! CCTATATATGACTACATTTA+TGG - Chr4:43660872-43660891 MsG0480020575.01.T01:five_prime_UTR 25.0%
! GGTTTACGAAAAATGTATAA+AGG + Chr4:43660128-43660147 None:intergenic 25.0%
! GTATGTGAAATACTTCAATT+GGG + Chr4:43660386-43660405 None:intergenic 25.0%
! TATGTGAAATACTTCAATTG+GGG + Chr4:43660385-43660404 None:intergenic 25.0%
! TGTATGTGAAATACTTCAAT+TGG + Chr4:43660387-43660406 None:intergenic 25.0%
! TTACATGTATATCACTTAGA+TGG + Chr4:43658587-43658606 None:intergenic 25.0%
! TTAGCCTAGAAAATTAAGAA+GGG + Chr4:43659204-43659223 None:intergenic 25.0%
! TTTAGCCTAGAAAATTAAGA+AGG + Chr4:43659205-43659224 None:intergenic 25.0%
! TTTATGTGGCTAATTCTAAT+TGG - Chr4:43658912-43658931 MsG0480020575.01.T01:intron 25.0%
!! ATAGAGTAAGTATTAGTTTG+CGG - Chr4:43658871-43658890 MsG0480020575.01.T01:intron 25.0%
!! TGGTTCCTAAATTTCAATTT+TGG - Chr4:43659528-43659547 MsG0480020575.01.T01:CDS 25.0%
!!! ATCGATTTTATGAACAACAT+GGG - Chr4:43660313-43660332 MsG0480020575.01.T01:intron 25.0%
!!! CATACAAAGTTTGATTTTAC+AGG - Chr4:43660798-43660817 MsG0480020575.01.T01:five_prime_UTR 25.0%
!!! CATACTTGTGTTTTAAATCT+TGG + Chr4:43661181-43661200 None:intergenic 25.0%
!!! CTTTTTCCCTTTATTTGTAT+TGG - Chr4:43660092-43660111 MsG0480020575.01.T01:intron 25.0%
!!! GAAGTTACTTTCTAAAACAA+TGG + Chr4:43661125-43661144 None:intergenic 25.0%
!!! TGACATATTGGTGTTATAAT+TGG + Chr4:43658467-43658486 None:intergenic 25.0%
AAAAAGGCCACATTAAATTG+AGG + Chr4:43661063-43661082 None:intergenic 30.0%
AAAAAGTTCATACCAGTGAT+AGG + Chr4:43660078-43660097 None:intergenic 30.0%
AATGGTACCTCAATTTAATG+TGG - Chr4:43661053-43661072 MsG0480020575.01.T01:five_prime_UTR 30.0%
ACTTTACGCAATATTTCCTA+TGG - Chr4:43659891-43659910 MsG0480020575.01.T01:intron 30.0%
AGAATAGCCTAGAAAAAACT+AGG - Chr4:43658963-43658982 MsG0480020575.01.T01:intron 30.0%
ATGAACAATGTTGTCAACAT+TGG + Chr4:43660250-43660269 None:intergenic 30.0%
CAGTCAACATCAATACTTTA+AGG + Chr4:43659243-43659262 None:intergenic 30.0%
GGAATATGAGATAGTATGTT+GGG + Chr4:43659380-43659399 None:intergenic 30.0%
GTTCATGAAAAAATCGTTTG+TGG - Chr4:43660264-43660283 MsG0480020575.01.T01:intron 30.0%
GTTTGTATTATCTGTTTCAC+CGG - Chr4:43660584-43660603 MsG0480020575.01.T01:intron 30.0%
TCTATTACTTTCTTGTTGAG+AGG + Chr4:43658624-43658643 None:intergenic 30.0%
TGAACAATGTTGTCAACATT+GGG + Chr4:43660249-43660268 None:intergenic 30.0%
TTTATTGTGTGACATTTGCA+GGG - Chr4:43659831-43659850 MsG0480020575.01.T01:intron 30.0%
! CACTTGCAAATTTGTTTATG+TGG - Chr4:43658898-43658917 MsG0480020575.01.T01:intron 30.0%
! CATATACTCTTTTATATGGG+TGG - Chr4:43659347-43659366 MsG0480020575.01.T01:intron 30.0%
! GTTTTCCTTAACTAATGTCT+CGG + Chr4:43659085-43659104 None:intergenic 30.0%
! TTCTTTTTCCTTTAGCAGTT+TGG + Chr4:43659458-43659477 None:intergenic 30.0%
! TTTTATTGTGTGACATTTGC+AGG - Chr4:43659830-43659849 MsG0480020575.01.T01:intron 30.0%
! TTTTCCTTAACTAATGTCTC+GGG + Chr4:43659084-43659103 None:intergenic 30.0%
!! AAACTTATTGTTGGTGTTGT+TGG - Chr4:43660158-43660177 MsG0480020575.01.T01:intron 30.0%
!! TAGAGTAAGTATTAGTTTGC+GGG - Chr4:43658872-43658891 MsG0480020575.01.T01:intron 30.0%
!!! CATCGATTTTATGAACAACA+TGG - Chr4:43660312-43660331 MsG0480020575.01.T01:intron 30.0%
!!! TTGTTGGTTTTTTATGACAG+CGG - Chr4:43660174-43660193 MsG0480020575.01.T01:intron 30.0%
AAGTAGGAGAAGAGTTTCTT+AGG + Chr4:43660361-43660380 None:intergenic 35.0%
AGGTACCATTTAGATGTTTG+AGG + Chr4:43661043-43661062 None:intergenic 35.0%
ATACTTCAATTGGGGAAAGT+AGG + Chr4:43660377-43660396 None:intergenic 35.0%
CAGTGACAATTGAAGAATGA+TGG + Chr4:43660053-43660072 None:intergenic 35.0%
CGAACTTAGTGTTCATAATC+AGG + Chr4:43659299-43659318 None:intergenic 35.0%
CTAAAATGCGGAAGAATTCT+AGG - Chr4:43658799-43658818 MsG0480020575.01.T01:intron 35.0%
GAATGTTGCACAATCAGATA+TGG - Chr4:43659415-43659434 MsG0480020575.01.T01:CDS 35.0%
GGGAATATGAGATAGTATGT+TGG + Chr4:43659381-43659400 None:intergenic 35.0%
GGTACCATTTAGATGTTTGA+GGG + Chr4:43661042-43661061 None:intergenic 35.0%
GTTGTAATGCAAACTTACGA+TGG + Chr4:43660412-43660431 None:intergenic 35.0%
TATATGCTATGAGCAATCCA+CGG + Chr4:43659333-43659352 None:intergenic 35.0%
TATGCCAACATTTCAGCAAA+TGG + Chr4:43660208-43660227 None:intergenic 35.0%
TGACTACATTTATGGTACTG+TGG - Chr4:43660880-43660899 MsG0480020575.01.T01:five_prime_UTR 35.0%
! ACATCCATTTGCTGAAATGT+TGG - Chr4:43660201-43660220 MsG0480020575.01.T01:intron 35.0%
! AGTGCCCTTCTTAATTTTCT+AGG - Chr4:43659197-43659216 MsG0480020575.01.T01:CDS 35.0%
! CTAGAATTCTTCCGCATTTT+AGG + Chr4:43658801-43658820 None:intergenic 35.0%
! CTTTAAGGAAAATGCTACTG+TGG + Chr4:43659228-43659247 None:intergenic 35.0%
! TTGAAGAATGATGGTGAGAA+TGG + Chr4:43660044-43660063 None:intergenic 35.0%
!! AAACTAAAGGTACCCTATGT+AGG + Chr4:43660568-43660587 None:intergenic 35.0%
!!! TTCCAATTATTTTAGGGGTG+TGG - Chr4:43660448-43660467 MsG0480020575.01.T01:intron 35.0%
AAGAATTGTTGACAAAGGCC+TGG - Chr4:43659476-43659495 MsG0480020575.01.T01:CDS 40.0%
AAGTCGACAGAGAAACCAAT+TGG - Chr4:43659508-43659527 MsG0480020575.01.T01:CDS 40.0%
AGCATAGAGCACGTTGAAAA+AGG + Chr4:43661079-43661098 None:intergenic 40.0%
AGCTTCATATGTAGCATCTG+AGG + Chr4:43660912-43660931 None:intergenic 40.0%
ATATGCTATGAGCAATCCAC+GGG + Chr4:43659332-43659351 None:intergenic 40.0%
ATTCAGCGAGACCTAAAATG+CGG - Chr4:43658787-43658806 MsG0480020575.01.T01:intron 40.0%
CACCACACCCCTAAAATAAT+TGG + Chr4:43660453-43660472 None:intergenic 40.0%
CAGTAGTACAAGAATTCCAC+TGG + Chr4:43659979-43659998 None:intergenic 40.0%
CAGTGGAATTCTTGTACTAC+TGG - Chr4:43659977-43659996 MsG0480020575.01.T01:intron 40.0%
CCAACCCTCAAACATCTAAA+TGG - Chr4:43661035-43661054 MsG0480020575.01.T01:five_prime_UTR 40.0%
CCATCTTTCAACTACGCTTA+GGG - Chr4:43660996-43661015 MsG0480020575.01.T01:five_prime_UTR 40.0%
CCATTTAGATGTTTGAGGGT+TGG + Chr4:43661038-43661057 None:intergenic 40.0%
CGCTGCATCACACAAAATTT+AGG - Chr4:43660828-43660847 MsG0480020575.01.T01:five_prime_UTR 40.0%
TCATACTATGGCTTCCAAAG+CGG - Chr4:43658267-43658286 MsG0480020575.01.T01:CDS 40.0%
TCCATCTTTCAACTACGCTT+AGG - Chr4:43660995-43661014 MsG0480020575.01.T01:five_prime_UTR 40.0%
TGTTCTCCACCATTGTAAGT+TGG + Chr4:43659920-43659939 None:intergenic 40.0%
TTGGCTAGATGTTGTGTGTA+AGG - Chr4:43660498-43660517 MsG0480020575.01.T01:intron 40.0%
TTTAGATGTTTGAGGGTTGG+AGG + Chr4:43661035-43661054 None:intergenic 40.0%
! AATTGGTTTCTCTGTCGACT+TGG + Chr4:43659509-43659528 None:intergenic 40.0%
! ATGCTTGTGACTTGGATTAG+TGG - Chr4:43661094-43661113 MsG0480020575.01.T01:five_prime_UTR 40.0%
! TAATTTGTGGAAAGGGTCGA+GGG + Chr4:43658425-43658444 None:intergenic 40.0%
! TTACAATGGTGGAGAACAGA+TGG - Chr4:43659922-43659941 MsG0480020575.01.T01:intron 40.0%
!! TGCTTGTGACTTGGATTAGT+GGG - Chr4:43661095-43661114 MsG0480020575.01.T01:five_prime_UTR 40.0%
!! TTAATTTGTGGAAAGGGTCG+AGG + Chr4:43658426-43658445 None:intergenic 40.0%
AAGATCTCAAGCTGCAGTGT+TGG - Chr4:43661149-43661168 MsG0480020575.01.T01:five_prime_UTR 45.0%
AATTTGTGGAAAGGGTCGAG+GGG + Chr4:43658424-43658443 None:intergenic 45.0%
ATTGTCACTGAGCCTATCAC+TGG - Chr4:43660063-43660082 MsG0480020575.01.T01:intron 45.0%
CATACTATGGCTTCCAAAGC+GGG - Chr4:43658268-43658287 MsG0480020575.01.T01:CDS 45.0%
CCCTAAGCGTAGTTGAAAGA+TGG + Chr4:43660999-43661018 None:intergenic 45.0%
CTATGGCTGCCAACTTACAA+TGG - Chr4:43659908-43659927 MsG0480020575.01.T01:intron 45.0%
GTGTCCCGAGACATTAGTTA+AGG - Chr4:43659077-43659096 MsG0480020575.01.T01:CDS 45.0%
GTTTCCAAGGCCAGTTTGTA+AGG + Chr4:43658297-43658316 None:intergenic 45.0%
TACTCAAGACGAACAGAGAC+CGG + Chr4:43660606-43660625 None:intergenic 45.0%
TCACGCTACCAAACTGCTAA+AGG - Chr4:43659447-43659466 MsG0480020575.01.T01:CDS 45.0%
TGTGTTAAGTGCACCACATC+TGG + Chr4:43660959-43660978 None:intergenic 45.0%
! CATTGTAAGTTGGCAGCCAT+AGG + Chr4:43659910-43659929 None:intergenic 45.0%
!! CTAAAGGTACCCTATGTAGG+CGG + Chr4:43660565-43660584 None:intergenic 45.0%
AAGCGGGCATCCTTACAAAC+TGG - Chr4:43658284-43658303 MsG0480020575.01.T01:CDS 50.0%
AAGCTCCCAAGAGGTTTCCA+AGG + Chr4:43658310-43658329 None:intergenic 50.0%
ACAACCTTGAAGCTCCCAAG+AGG + Chr4:43658319-43658338 None:intergenic 50.0%
ACCACATCTGGTGATTCCTC+TGG + Chr4:43660947-43660966 None:intergenic 50.0%
ACCAGAGGAATCACCAGATG+TGG - Chr4:43660943-43660962 MsG0480020575.01.T01:five_prime_UTR 50.0%
AGCTTGTGTGAAGAGACCAG+AGG - Chr4:43660928-43660947 MsG0480020575.01.T01:five_prime_UTR 50.0%
AGTTCGTGATTCTAAGCCCG+TGG - Chr4:43659313-43659332 MsG0480020575.01.T01:intron 50.0%
CCTACGTGCAACATCCATGA+GGG + Chr4:43660476-43660495 None:intergenic 50.0%
CCTCATGGATGTTGCACGTA+GGG - Chr4:43660474-43660493 MsG0480020575.01.T01:intron 50.0%
CGTGCTCTATGCTTGTGACT+TGG - Chr4:43661086-43661105 MsG0480020575.01.T01:five_prime_UTR 50.0%
CTTACAGCTCTCTTGCATGC+TGG - Chr4:43659952-43659971 MsG0480020575.01.T01:intron 50.0%
GAAACCTCTTGGGAGCTTCA+AGG - Chr4:43658312-43658331 MsG0480020575.01.T01:CDS 50.0%
GTTTGTAAGGATGCCCGCTT+TGG + Chr4:43658284-43658303 None:intergenic 50.0%
TCGTTTGTGGCTGCACTGTA+TGG - Chr4:43660277-43660296 MsG0480020575.01.T01:intron 50.0%
TGGATGTTGCACGTAGGGTT+TGG - Chr4:43660479-43660498 MsG0480020575.01.T01:intron 50.0%
TGGCTGCCAACTTACAATGG+TGG - Chr4:43659911-43659930 MsG0480020575.01.T01:intron 50.0%
TGTTTGAGGGTTGGAGGCTA+AGG + Chr4:43661029-43661048 None:intergenic 50.0%
! GCATCCTTACAAACTGGCCT+TGG - Chr4:43658290-43658309 MsG0480020575.01.T01:CDS 50.0%
!! AACTGGCCTTGGAAACCTCT+TGG - Chr4:43658301-43658320 MsG0480020575.01.T01:CDS 50.0%
!! ACTGGCCTTGGAAACCTCTT+GGG - Chr4:43658302-43658321 MsG0480020575.01.T01:CDS 50.0%
!! TGTCGACTTGGTCGAATTCC+AGG + Chr4:43659497-43659516 None:intergenic 50.0%
CCCTACGTGCAACATCCATG+AGG + Chr4:43660477-43660496 None:intergenic 55.0%
CCCTCATGGATGTTGCACGT+AGG - Chr4:43660473-43660492 MsG0480020575.01.T01:intron 55.0%
TCGGGTGTTCCGCCTACATA+GGG - Chr4:43660553-43660572 MsG0480020575.01.T01:intron 55.0%
TCTCTTGCATGCTGGACCAG+TGG - Chr4:43659960-43659979 MsG0480020575.01.T01:intron 55.0%
TTCGGGTGTTCCGCCTACAT+AGG - Chr4:43660552-43660571 MsG0480020575.01.T01:intron 55.0%
! TTAGGGGTGTGGTGCCCTCA+TGG - Chr4:43660459-43660478 MsG0480020575.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 43658258 43661201 43658258 ID=MsG0480020575.01;Name=MsG0480020575.01
Chr4 mRNA 43658258 43661201 43658258 ID=MsG0480020575.01.T01;Parent=MsG0480020575.01;Name=MsG0480020575.01.T01;_AED=0.35;_eAED=0.35;_QI=459|0.75|0.8|1|0.75|0.6|5|0|283
Chr4 exon 43658258 43658639 43658258 ID=MsG0480020575.01.T01:exon:17263;Parent=MsG0480020575.01.T01
Chr4 exon 43659046 43659265 43659046 ID=MsG0480020575.01.T01:exon:17262;Parent=MsG0480020575.01.T01
Chr4 exon 43659375 43659607 43659375 ID=MsG0480020575.01.T01:exon:17261;Parent=MsG0480020575.01.T01
Chr4 exon 43660639 43660697 43660639 ID=MsG0480020575.01.T01:exon:17260;Parent=MsG0480020575.01.T01
Chr4 exon 43660785 43661201 43660785 ID=MsG0480020575.01.T01:exon:17259;Parent=MsG0480020575.01.T01
Chr4 five_prime_UTR 43660785 43661201 43660785 ID=MsG0480020575.01.T01:five_prime_utr;Parent=MsG0480020575.01.T01
Chr4 five_prime_UTR 43660656 43660697 43660656 ID=MsG0480020575.01.T01:five_prime_utr;Parent=MsG0480020575.01.T01
Chr4 CDS 43660639 43660655 43660639 ID=MsG0480020575.01.T01:cds;Parent=MsG0480020575.01.T01
Chr4 CDS 43659375 43659607 43659375 ID=MsG0480020575.01.T01:cds;Parent=MsG0480020575.01.T01
Chr4 CDS 43659046 43659265 43659046 ID=MsG0480020575.01.T01:cds;Parent=MsG0480020575.01.T01
Chr4 CDS 43658258 43658639 43658258 ID=MsG0480020575.01.T01:cds;Parent=MsG0480020575.01.T01
Gene Sequence

>MsG0480020575.01.T01

ATGCGGAAGAATTCTAGGGATGATAAAATATTGTTCACTGCATTAATGTTTTATATACTTTACGCAATATTTCCTATGGCTGCCAACTTACAATGGTGGAGAACAGATGGTGTGATTCTTACAGCTCTCTTGCATGCTGGACCAGTGGAATTCTTGTACTACTGGCTACATAGAGCACTTCATCATCATTATCTATATTCTCGTTACCATTCTCACCATCATTCTTCAATTGTCACTGAGCCTATCACTGCGGTGACACATCCATTTGCTGAAATGTTGGCATATTTCACACTTTTTCTTATCCCAATGTTGACAACATTGTTCATGAAAAAATCGTTTGTGGCTGCACTGTATGGTTATATCTTTTACATCGATTTTATGAACAACATGGGTCATTGCAATTTTGAGTTCTTTCCTAAGAAACTCTTCTCCTACTTTCCCCAATTGAAGTATTTCACATACACACCATCGTTTCACTCGCTGCATCACACAAAATTTAGGTCAAATTACTCACTTTTCATGCCTATATATGACTACATTTATGGTACTGTGGATGCATCCTCAGATGCTACATATGAAGCTTGTGTGAAGAGACCAGAGGAATCACCAGATGTGGTGCACTTAACACATTTAACAACACTTGATTCCATCTTTCAACTACGCTTAGGGTTTGCTTCCTTAGCCTCCAACCCTCAAACATCTAAATGGTACCTCAATTTAATGTGGCCTTTTTCAACGTGCTCTATGCTTGTGACTTGGATTAGTGGGCATGCCATTGTTTTAGAAAGTAACTTCTTCAAAGATCTCAAGCTGCAGTGTTGGCTTATACCAAGATTTAAAACACAAGTATGA
Protein sequence

>MsG0480020575.01.T01

MRKNSRDDKILFTALMFYILYAIFPMAANLQWWRTDGVILTALLHAGPVEFLYYWLHRALHHHYLYSRYHSHHHSSIVTEPITAVTHPFAEMLAYFTLFLIPMLTTLFMKKSFVAALYGYIFYIDFMNNMGHCNFEFFPKKLFSYFPQLKYFTYTPSFHSLHHTKFRSNYSLFMPIYDYIYGTVDASSDATYEACVKRPEESPDVVHLTHLTTLDSIFQLRLGFASLASNPQTSKWYLNLMWPFSTCSMLVTWISGHAIVLESNFFKDLKLQCWLIPRFKTQV*