Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036621.01.T01 | RHN44465.1 | 87.437 | 199 | 25 | 0 | 1 | 199 | 202 | 400 | 7.27E-123 | 369 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036621.01.T01 | Q9SCZ4 | 42.262 | 168 | 93 | 2 | 29 | 194 | 217 | 382 | 4.44E-35 | 134 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036621.01.T01 | A0A396H030 | 87.437 | 199 | 25 | 0 | 1 | 199 | 202 | 400 | 3.47e-123 | 369 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480018677.01 | MsG0780036621.01 | 0.809809 | 1.574591e-50 | 9.336604e-48 |
| MsG0480020574.01 | MsG0780036621.01 | 0.814592 | 1.424724e-51 | 9.599641e-49 |
| MsG0480020575.01 | MsG0780036621.01 | 0.847865 | 8.823068e-60 | 1.589701e-56 |
| MsG0580024447.01 | MsG0780036621.01 | 0.803886 | 2.814861e-49 | 1.430967e-46 |
| MsG0380017855.01 | MsG0780036621.01 | 0.811205 | 7.863784e-51 | 4.837945e-48 |
| MsG0580027955.01 | MsG0780036621.01 | 0.839938 | 1.174575e-57 | 1.647350e-54 |
| MsG0280006873.01 | MsG0780036621.01 | 0.806102 | 9.680116e-50 | 5.209287e-47 |
| MsG0780036621.01 | MsG0880044615.01 | 0.804904 | 1.726542e-49 | 9.010750e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036621.01.T01 | MTR_7g015620 | 86.432 | 199 | 27 | 0 | 1 | 199 | 198 | 396 | 5.28e-122 | 360 |
| MsG0780036621.01.T01 | MTR_7g015420 | 79.899 | 199 | 40 | 0 | 1 | 199 | 198 | 396 | 9.53e-108 | 333 |
| MsG0780036621.01.T01 | MTR_7g015230 | 72.362 | 199 | 54 | 1 | 1 | 199 | 208 | 405 | 4.89e-100 | 308 |
| MsG0780036621.01.T01 | MTR_7g015310 | 72.500 | 200 | 54 | 1 | 1 | 199 | 202 | 401 | 5.46e-99 | 305 |
| MsG0780036621.01.T01 | MTR_7g015320 | 71.154 | 208 | 50 | 2 | 1 | 199 | 203 | 409 | 1.81e-97 | 301 |
| MsG0780036621.01.T01 | MTR_4g052290 | 70.352 | 199 | 58 | 1 | 1 | 199 | 201 | 398 | 2.57e-95 | 296 |
| MsG0780036621.01.T01 | MTR_7g015670 | 69.849 | 199 | 59 | 1 | 1 | 199 | 204 | 401 | 2.71e-93 | 291 |
| MsG0780036621.01.T01 | MTR_7g015510 | 69.347 | 199 | 60 | 1 | 1 | 199 | 205 | 402 | 1.78e-89 | 280 |
| MsG0780036621.01.T01 | MTR_7g015390 | 67.839 | 199 | 63 | 1 | 1 | 199 | 205 | 402 | 5.00e-89 | 279 |
| MsG0780036621.01.T01 | MTR_7g015280 | 68.844 | 199 | 53 | 2 | 1 | 199 | 202 | 391 | 4.12e-88 | 276 |
| MsG0780036621.01.T01 | MTR_7g015550 | 67.839 | 199 | 63 | 1 | 1 | 199 | 205 | 402 | 3.04e-86 | 272 |
| MsG0780036621.01.T01 | MTR_7g015250 | 63.981 | 211 | 63 | 3 | 1 | 199 | 197 | 406 | 5.95e-85 | 269 |
| MsG0780036621.01.T01 | MTR_7g015240 | 67.513 | 197 | 61 | 2 | 1 | 197 | 201 | 394 | 1.03e-83 | 265 |
| MsG0780036621.01.T01 | MTR_4g111925 | 39.894 | 188 | 95 | 6 | 17 | 193 | 205 | 385 | 1.48e-33 | 126 |
| MsG0780036621.01.T01 | MTR_7g073660 | 39.894 | 188 | 95 | 6 | 17 | 193 | 205 | 385 | 1.77e-33 | 126 |
| MsG0780036621.01.T01 | MTR_6g015805 | 38.624 | 189 | 102 | 7 | 1 | 183 | 191 | 371 | 4.34e-32 | 122 |
| MsG0780036621.01.T01 | MTR_6g015795 | 39.153 | 189 | 104 | 7 | 1 | 183 | 194 | 377 | 8.21e-32 | 121 |
| MsG0780036621.01.T01 | MTR_5g047110 | 40.805 | 174 | 83 | 6 | 19 | 183 | 211 | 373 | 6.33e-30 | 116 |
| MsG0780036621.01.T01 | MTR_5g047060 | 41.104 | 163 | 79 | 5 | 29 | 183 | 221 | 374 | 3.47e-29 | 114 |
| MsG0780036621.01.T01 | MTR_1g080740 | 33.155 | 187 | 117 | 5 | 10 | 194 | 188 | 368 | 1.77e-26 | 106 |
| MsG0780036621.01.T01 | MTR_7g115300 | 36.111 | 180 | 103 | 8 | 17 | 193 | 205 | 375 | 1.85e-24 | 100 |
| MsG0780036621.01.T01 | MTR_5g047120 | 31.250 | 160 | 102 | 3 | 29 | 183 | 228 | 384 | 3.35e-19 | 85.1 |
| MsG0780036621.01.T01 | MTR_5g047070 | 37.634 | 93 | 55 | 1 | 94 | 183 | 51 | 143 | 1.02e-16 | 78.2 |
| MsG0780036621.01.T01 | MTR_2g030310 | 31.579 | 152 | 92 | 5 | 34 | 181 | 220 | 363 | 1.78e-16 | 77.4 |
| MsG0780036621.01.T01 | MTR_2g030310 | 31.579 | 152 | 92 | 5 | 34 | 181 | 220 | 363 | 1.95e-16 | 77.4 |
| MsG0780036621.01.T01 | MTR_2g080220 | 31.319 | 182 | 117 | 6 | 10 | 189 | 183 | 358 | 6.60e-15 | 72.8 |
| MsG0780036621.01.T01 | MTR_8g037700 | 30.723 | 166 | 106 | 5 | 34 | 194 | 238 | 399 | 1.51e-14 | 71.6 |
| MsG0780036621.01.T01 | MTR_2g096160 | 29.448 | 163 | 103 | 5 | 33 | 189 | 208 | 364 | 5.85e-13 | 67.0 |
| MsG0780036621.01.T01 | MTR_4g061833 | 31.056 | 161 | 99 | 5 | 10 | 166 | 36 | 188 | 1.07e-12 | 66.2 |
| MsG0780036621.01.T01 | MTR_4g061833 | 31.056 | 161 | 99 | 5 | 10 | 166 | 187 | 339 | 1.39e-12 | 66.2 |
| MsG0780036621.01.T01 | MTR_4g061930 | 31.056 | 161 | 99 | 5 | 10 | 166 | 187 | 339 | 1.39e-12 | 66.2 |
| MsG0780036621.01.T01 | MTR_8g467150 | 31.206 | 141 | 81 | 5 | 31 | 162 | 202 | 335 | 2.52e-12 | 65.5 |
| MsG0780036621.01.T01 | MTR_6g048090 | 31.655 | 139 | 82 | 7 | 7 | 137 | 181 | 314 | 6.69e-12 | 63.9 |
| MsG0780036621.01.T01 | MTR_1g100110 | 29.940 | 167 | 104 | 7 | 7 | 166 | 190 | 350 | 7.39e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036621.01.T01 | AT3G51550 | 42.262 | 168 | 93 | 2 | 29 | 194 | 217 | 382 | 4.53e-36 | 134 |
| MsG0780036621.01.T01 | AT5G39000 | 41.176 | 170 | 88 | 5 | 26 | 190 | 211 | 373 | 1.81e-30 | 117 |
| MsG0780036621.01.T01 | AT5G38990 | 41.860 | 172 | 92 | 5 | 26 | 193 | 205 | 372 | 7.71e-30 | 115 |
| MsG0780036621.01.T01 | AT5G39020 | 38.587 | 184 | 98 | 5 | 21 | 195 | 195 | 372 | 5.85e-29 | 113 |
| MsG0780036621.01.T01 | AT5G28680 | 35.135 | 185 | 108 | 6 | 17 | 195 | 194 | 372 | 1.34e-27 | 109 |
| MsG0780036621.01.T01 | AT5G28680 | 35.135 | 185 | 108 | 6 | 17 | 195 | 194 | 372 | 1.34e-27 | 109 |
| MsG0780036621.01.T01 | AT5G39030 | 38.596 | 171 | 90 | 6 | 26 | 190 | 207 | 368 | 1.44e-27 | 109 |
| MsG0780036621.01.T01 | AT5G39030 | 38.596 | 171 | 90 | 6 | 26 | 190 | 201 | 362 | 1.73e-27 | 109 |
| MsG0780036621.01.T01 | AT3G04690 | 34.783 | 184 | 108 | 7 | 17 | 194 | 193 | 370 | 5.72e-27 | 107 |
| MsG0780036621.01.T01 | AT1G30570 | 32.716 | 162 | 95 | 6 | 33 | 189 | 223 | 375 | 4.38e-21 | 90.9 |
| MsG0780036621.01.T01 | AT5G59700 | 37.500 | 128 | 72 | 4 | 33 | 158 | 197 | 318 | 7.26e-16 | 75.9 |
| MsG0780036621.01.T01 | AT5G54380 | 34.568 | 162 | 93 | 7 | 34 | 193 | 207 | 357 | 7.55e-15 | 72.8 |
| MsG0780036621.01.T01 | AT4G00300 | 33.987 | 153 | 92 | 7 | 33 | 183 | 210 | 355 | 1.60e-14 | 71.6 |
| MsG0780036621.01.T01 | AT2G21480 | 33.708 | 178 | 110 | 6 | 8 | 183 | 209 | 380 | 1.97e-14 | 71.6 |
| MsG0780036621.01.T01 | AT4G39110 | 32.022 | 178 | 113 | 5 | 8 | 183 | 210 | 381 | 2.01e-14 | 71.6 |
| MsG0780036621.01.T01 | AT3G46290 | 31.884 | 138 | 82 | 5 | 33 | 166 | 198 | 327 | 3.81e-12 | 64.7 |
Find 36 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATATTTATCGATGATATTT+TGG | 0.161759 | 7:+10794517 | MsG0780036621.01.T01:CDS |
| AATTATCATTCCAATTTAAT+TGG | 0.178321 | 7:+10794101 | MsG0780036621.01.T01:CDS |
| CTTAGTGTATCAAGTGTTTA+TGG | 0.182673 | 7:+10794281 | MsG0780036621.01.T01:CDS |
| ATACAGGTATGTTTCGTAAT+TGG | 0.195211 | 7:+10794219 | MsG0780036621.01.T01:CDS |
| ACTTCCGGTGCAGTGTAATT+TGG | 0.202010 | 7:-10794338 | None:intergenic |
| GTTGTCAAAGCCAATTAAAT+TGG | 0.277748 | 7:-10794111 | None:intergenic |
| GTGAAAATGTACTCTCAAAA+TGG | 0.301581 | 7:+10794608 | MsG0780036621.01.T01:CDS |
| GAATGATAATTTAGATCATC+TGG | 0.313122 | 7:-10794089 | None:intergenic |
| CAATGATTATCCTGGTTACT+TGG | 0.345911 | 7:+10794244 | MsG0780036621.01.T01:CDS |
| TACAGGTATGTTTCGTAATT+GGG | 0.359203 | 7:+10794220 | MsG0780036621.01.T01:CDS |
| TACTTTACTGCAAGAAGTTA+TGG | 0.359689 | 7:+10794365 | MsG0780036621.01.T01:CDS |
| GATCCGCTTATCAAGAATAA+AGG | 0.374070 | 7:+10794479 | MsG0780036621.01.T01:CDS |
| TAACATGATTCCTGTTCATA+AGG | 0.412064 | 7:+10794577 | MsG0780036621.01.T01:CDS |
| GCAGTAAAGTATACAACTTC+CGG | 0.414772 | 7:-10794353 | None:intergenic |
| CACCTTATCACATCGGCATC+TGG | 0.417815 | 7:-10794545 | None:intergenic |
| ACAGCATAATCCTTATGAAC+AGG | 0.433445 | 7:-10794587 | None:intergenic |
| AATTCGTAATGATAAAGCTT+TGG | 0.433961 | 7:+10794145 | MsG0780036621.01.T01:CDS |
| CAGAGGATTATAATGTAACT+TGG | 0.462446 | 7:+10794399 | MsG0780036621.01.T01:CDS |
| TTTGTGTAATAGAGAAAAGA+AGG | 0.500353 | 7:-10794065 | None:intergenic |
| AATTGGGACAATGATTATCC+TGG | 0.511473 | 7:+10794236 | MsG0780036621.01.T01:CDS |
| AACCAGATGCCGATGTGATA+AGG | 0.515610 | 7:+10794543 | MsG0780036621.01.T01:CDS |
| CGGTGCAGTGTAATTTGGAA+TGG | 0.521014 | 7:-10794333 | None:intergenic |
| GTTGAACCAAGTGATGATAC+AGG | 0.538055 | 7:+10794203 | MsG0780036621.01.T01:CDS |
| AATGATAATTTAGATCATCT+GGG | 0.541109 | 7:-10794088 | None:intergenic |
| TCACCTTTATTCTTGATAAG+CGG | 0.547794 | 7:-10794482 | None:intergenic |
| TAATGATAAAGCTTTGGAGA+TGG | 0.561387 | 7:+10794151 | MsG0780036621.01.T01:CDS |
| TATTGTTTCTCCAAGTAACC+AGG | 0.569783 | 7:-10794254 | None:intergenic |
| AACATACCTGTATCATCACT+TGG | 0.571312 | 7:-10794209 | None:intergenic |
| CATTCCAAATTACACTGCAC+CGG | 0.592742 | 7:+10794334 | MsG0780036621.01.T01:CDS |
| CAGATGCCGATGTGATAAGG+TGG | 0.633194 | 7:+10794546 | MsG0780036621.01.T01:CDS |
| ACCACTCCACCTTATCACAT+CGG | 0.660024 | 7:-10794552 | None:intergenic |
| ATGGTTTATAGAGTCAACGT+TGG | 0.674503 | 7:+10794170 | MsG0780036621.01.T01:CDS |
| GCCGATGTGATAAGGTGGAG+TGG | 0.694565 | 7:+10794551 | MsG0780036621.01.T01:CDS |
| AGTTACATTATAATCCTCTG+TGG | 0.712355 | 7:-10794396 | None:intergenic |
| GATGTGATAAGGTGGAGTGG+TGG | 0.740563 | 7:+10794554 | MsG0780036621.01.T01:CDS |
| TTATGGAATGAATGCCACAG+AGG | 0.742727 | 7:+10794382 | MsG0780036621.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATCATTCCAATTTAAT+TGG | + | Chr7:10794101-10794120 | MsG0780036621.01.T01:CDS | 15.0% |
| !! | AATGATAATTTAGATCATCT+GGG | - | Chr7:10794091-10794110 | None:intergenic | 20.0% |
| !!! | CTTCTTTTACTTATATGATA+AGG | + | Chr7:10794438-10794457 | MsG0780036621.01.T01:CDS | 20.0% |
| !!! | GATATTTATCGATGATATTT+TGG | + | Chr7:10794517-10794536 | MsG0780036621.01.T01:CDS | 20.0% |
| ! | AATTCGTAATGATAAAGCTT+TGG | + | Chr7:10794145-10794164 | MsG0780036621.01.T01:CDS | 25.0% |
| ! | GAATGATAATTTAGATCATC+TGG | - | Chr7:10794092-10794111 | None:intergenic | 25.0% |
| ! | TTTGTGTAATAGAGAAAAGA+AGG | - | Chr7:10794068-10794087 | None:intergenic | 25.0% |
| AGTTACATTATAATCCTCTG+TGG | - | Chr7:10794399-10794418 | None:intergenic | 30.0% | |
| ATACAGGTATGTTTCGTAAT+TGG | + | Chr7:10794219-10794238 | MsG0780036621.01.T01:CDS | 30.0% | |
| CAGAGGATTATAATGTAACT+TGG | + | Chr7:10794399-10794418 | MsG0780036621.01.T01:CDS | 30.0% | |
| CTTAGTGTATCAAGTGTTTA+TGG | + | Chr7:10794281-10794300 | MsG0780036621.01.T01:CDS | 30.0% | |
| GTGAAAATGTACTCTCAAAA+TGG | + | Chr7:10794608-10794627 | MsG0780036621.01.T01:CDS | 30.0% | |
| GTTGTCAAAGCCAATTAAAT+TGG | - | Chr7:10794114-10794133 | None:intergenic | 30.0% | |
| TAACATGATTCCTGTTCATA+AGG | + | Chr7:10794577-10794596 | MsG0780036621.01.T01:CDS | 30.0% | |
| TACAGGTATGTTTCGTAATT+GGG | + | Chr7:10794220-10794239 | MsG0780036621.01.T01:CDS | 30.0% | |
| TACTTTACTGCAAGAAGTTA+TGG | + | Chr7:10794365-10794384 | MsG0780036621.01.T01:CDS | 30.0% | |
| TCACCTTTATTCTTGATAAG+CGG | - | Chr7:10794485-10794504 | None:intergenic | 30.0% | |
| !! | TAATGATAAAGCTTTGGAGA+TGG | + | Chr7:10794151-10794170 | MsG0780036621.01.T01:CDS | 30.0% |
| AACATACCTGTATCATCACT+TGG | - | Chr7:10794212-10794231 | None:intergenic | 35.0% | |
| AATTGGGACAATGATTATCC+TGG | + | Chr7:10794236-10794255 | MsG0780036621.01.T01:CDS | 35.0% | |
| ACAGCATAATCCTTATGAAC+AGG | - | Chr7:10794590-10794609 | None:intergenic | 35.0% | |
| ATGGTTTATAGAGTCAACGT+TGG | + | Chr7:10794170-10794189 | MsG0780036621.01.T01:CDS | 35.0% | |
| CAATGATTATCCTGGTTACT+TGG | + | Chr7:10794244-10794263 | MsG0780036621.01.T01:CDS | 35.0% | |
| GATCCGCTTATCAAGAATAA+AGG | + | Chr7:10794479-10794498 | MsG0780036621.01.T01:CDS | 35.0% | |
| GCAGTAAAGTATACAACTTC+CGG | - | Chr7:10794356-10794375 | None:intergenic | 35.0% | |
| ! | TATTGTTTCTCCAAGTAACC+AGG | - | Chr7:10794257-10794276 | None:intergenic | 35.0% |
| CATTCCAAATTACACTGCAC+CGG | + | Chr7:10794334-10794353 | MsG0780036621.01.T01:CDS | 40.0% | |
| GTTGAACCAAGTGATGATAC+AGG | + | Chr7:10794203-10794222 | MsG0780036621.01.T01:CDS | 40.0% | |
| TTATGGAATGAATGCCACAG+AGG | + | Chr7:10794382-10794401 | MsG0780036621.01.T01:CDS | 40.0% | |
| AACCAGATGCCGATGTGATA+AGG | + | Chr7:10794543-10794562 | MsG0780036621.01.T01:CDS | 45.0% | |
| ACCACTCCACCTTATCACAT+CGG | - | Chr7:10794555-10794574 | None:intergenic | 45.0% | |
| ACTTCCGGTGCAGTGTAATT+TGG | - | Chr7:10794341-10794360 | None:intergenic | 45.0% | |
| CGGTGCAGTGTAATTTGGAA+TGG | - | Chr7:10794336-10794355 | None:intergenic | 45.0% | |
| CACCTTATCACATCGGCATC+TGG | - | Chr7:10794548-10794567 | None:intergenic | 50.0% | |
| CAGATGCCGATGTGATAAGG+TGG | + | Chr7:10794546-10794565 | MsG0780036621.01.T01:CDS | 50.0% | |
| GATGTGATAAGGTGGAGTGG+TGG | + | Chr7:10794554-10794573 | MsG0780036621.01.T01:CDS | 50.0% | |
| GCCGATGTGATAAGGTGGAG+TGG | + | Chr7:10794551-10794570 | MsG0780036621.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 10794062 | 10794661 | 10794062 | ID=MsG0780036621.01;Name=MsG0780036621.01 |
| Chr7 | mRNA | 10794062 | 10794661 | 10794062 | ID=MsG0780036621.01.T01;Parent=MsG0780036621.01;Name=MsG0780036621.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|199 |
| Chr7 | exon | 10794062 | 10794661 | 10794062 | ID=MsG0780036621.01.T01:exon:2046;Parent=MsG0780036621.01.T01 |
| Chr7 | CDS | 10794062 | 10794661 | 10794062 | ID=MsG0780036621.01.T01:cds;Parent=MsG0780036621.01.T01 |
| Gene Sequence |
| Protein sequence |
