AlfalfaGEDB | MsG0580027955.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027955.01

KEGG: -
GO: GO:0003700 GO:0005634 GO:0006355
PFAM: PF01486

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027955.01.T01	XP_003615289.2	86.538	52	7	0	1	52	109	160	2.79E-18	85.1

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027955.01.T01	G7KBL2	86.538	52	7	0	1	52	109	160	1.33e-18	85.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047883.01	MsG0580027955.01	0.812557	3.993385e-51	2.547061e-48
MsG0080047884.01	MsG0580027955.01	0.800383	1.478938e-48	6.876932e-46
MsG0080047885.01	MsG0580027955.01	0.813138	2.978226e-51	1.929002e-48
MsG0080048660.01	MsG0580027955.01	0.835066	2.083325e-56	2.519070e-53
MsG0080048748.01	MsG0580027955.01	0.830165	3.423379e-55	3.578584e-52
MsG0080048849.01	MsG0580027955.01	0.825337	4.952298e-54	4.501115e-51
MsG0080049069.01	MsG0580027955.01	0.811233	7.756899e-51	4.775680e-48
MsG0180000129.01	MsG0580027955.01	0.807630	4.601018e-50	2.575511e-47
MsG0180000599.01	MsG0580027955.01	0.837950	3.840916e-57	5.068778e-54
MsG0180000977.01	MsG0580027955.01	0.808638	2.807637e-50	1.613647e-47
MsG0180001704.01	MsG0580027955.01	0.804283	2.327316e-49	1.195175e-46
MsG0180002700.01	MsG0580027955.01	0.833533	5.049601e-56	5.831776e-53
MsG0180004025.01	MsG0580027955.01	0.811148	8.092508e-51	4.971094e-48
MsG0180004765.01	MsG0580027955.01	0.817130	3.867786e-52	2.793048e-49
MsG0180005639.01	MsG0580027955.01	0.822306	2.541563e-53	2.119354e-50
MsG0180005729.01	MsG0580027955.01	0.807136	5.856674e-50	3.236475e-47
MsG0180005731.01	MsG0580027955.01	0.803825	2.897512e-49	1.470633e-46
MsG0180005953.01	MsG0580027955.01	0.812177	4.833051e-51	3.051440e-48
MsG0180006054.01	MsG0580027955.01	0.808678	2.752347e-50	1.583546e-47
MsG0180006096.01	MsG0580027955.01	0.816621	5.032884e-52	3.584126e-49
MsG0180006180.01	MsG0580027955.01	0.816398	5.645197e-52	3.995727e-49
MsG0280011255.01	MsG0580027955.01	0.825226	5.260122e-54	4.765947e-51
MsG0380012005.01	MsG0580027955.01	0.817281	3.577089e-52	2.593713e-49
MsG0380012564.01	MsG0580027955.01	0.830758	2.452127e-55	2.607960e-52
MsG0380012565.01	MsG0580027955.01	0.825580	4.339116e-54	3.970937e-51
MsG0380012567.01	MsG0580027955.01	0.804371	2.230849e-49	1.148258e-46
MsG0380014386.01	MsG0580027955.01	0.814614	1.408614e-51	9.496689e-49
MsG0480018677.01	MsG0580027955.01	0.813682	2.261802e-51	1.486863e-48
MsG0480020025.01	MsG0580027955.01	0.825120	5.573134e-54	5.034267e-51
MsG0480020574.01	MsG0580027955.01	0.808527	2.964297e-50	1.698889e-47
MsG0480020575.01	MsG0580027955.01	0.805808	1.116132e-49	5.961575e-47
MsG0480021561.01	MsG0580027955.01	0.806585	7.660370e-50	4.173379e-47
MsG0480022065.01	MsG0580027955.01	0.809830	1.558779e-50	9.247690e-48
MsG0480022359.01	MsG0580027955.01	0.802925	4.451446e-49	2.207746e-46
MsG0480023150.01	MsG0580027955.01	0.819513	1.116531e-52	8.610161e-50
MsG0480023190.01	MsG0580027955.01	0.807599	4.673276e-50	2.613677e-47
MsG0480023723.01	MsG0580027955.01	0.815648	8.299466e-52	5.755514e-49
MsG0480024030.01	MsG0580027955.01	0.815106	1.095672e-51	7.485315e-49
MsG0580024340.01	MsG0580027955.01	0.805818	1.110743e-49	5.934347e-47
MsG0580025467.01	MsG0580027955.01	0.838135	3.442584e-57	4.568180e-54
MsG0580025827.01	MsG0580027955.01	0.839770	1.299032e-57	1.812238e-54
MsG0380015533.01	MsG0580027955.01	0.806865	6.682882e-50	3.667378e-47
MsG0380015847.01	MsG0580027955.01	-0.809811	1.573196e-50	9.328739e-48
MsG0380015885.01	MsG0580027955.01	0.808213	3.459582e-50	1.966497e-47
MsG0380015891.01	MsG0580027955.01	0.855889	4.657696e-62	1.091552e-58
MsG0380017202.01	MsG0580027955.01	0.804781	1.832004e-49	9.530246e-47
MsG0380017338.01	MsG0580027955.01	0.805913	1.061155e-49	5.683063e-47
MsG0380017437.01	MsG0580027955.01	-0.816224	6.173406e-52	4.348983e-49
MsG0380017931.01	MsG0580027955.01	0.814223	1.718795e-51	1.146561e-48
MsG0480018426.01	MsG0580027955.01	0.808964	2.390988e-50	1.386097e-47
MsG0580027955.01	MsG0580027980.01	0.847234	1.315341e-59	2.322706e-56
MsG0580027955.01	MsG0580028208.01	0.845557	3.771402e-59	6.308784e-56
MsG0580027955.01	MsG0580028419.01	0.818227	2.188179e-52	1.628352e-49
MsG0580027955.01	MsG0580028428.01	0.817179	3.771985e-52	2.727478e-49
MsG0580027955.01	MsG0680030680.01	0.807199	5.678899e-50	3.143504e-47
MsG0580027955.01	MsG0680035439.01	0.814030	1.896271e-51	1.258381e-48
MsG0580027955.01	MsG0680035596.01	0.827477	1.530706e-54	1.479497e-51
MsG0580027955.01	MsG0680035707.01	0.807396	5.160539e-50	2.871249e-47
MsG0580027955.01	MsG0780035960.01	0.838052	3.614768e-57	4.785132e-54
MsG0580027955.01	MsG0780036069.01	0.820261	7.531722e-53	5.930324e-50
MsG0580027955.01	MsG0780036585.01	0.812678	3.757795e-51	2.404383e-48
MsG0580027955.01	MsG0780036620.01	0.823363	1.442154e-53	1.239003e-50
MsG0580027955.01	MsG0780036621.01	0.839938	1.174575e-57	1.647350e-54
MsG0580027955.01	MsG0780036978.01	0.817594	3.041608e-52	2.224484e-49
MsG0580027955.01	MsG0780039336.01	0.805506	1.291864e-49	6.846283e-47
MsG0580027955.01	MsG0780039438.01	0.814582	1.431951e-51	9.645720e-49
MsG0580027955.01	MsG0780039940.01	0.806129	9.555503e-50	5.145828e-47
MsG0580027955.01	MsG0780039964.01	0.804209	2.410773e-49	1.235676e-46
MsG0580027955.01	MsG0780040278.01	0.804500	2.096604e-49	1.082698e-46
MsG0580027955.01	MsG0780041747.01	0.832858	7.438248e-56	8.418699e-53
MsG0580027955.01	MsG0880041864.01	0.807898	4.035865e-50	2.274916e-47
MsG0580027955.01	MsG0880042086.01	0.839768	1.300792e-57	1.814510e-54
MsG0580027955.01	MsG0880042416.01	0.825268	5.141875e-54	4.664267e-51
MsG0580027955.01	MsG0880042417.01	0.802201	6.278155e-49	3.056670e-46
MsG0580027955.01	MsG0880042622.01	0.801148	1.032433e-48	4.894666e-46
MsG0580027955.01	MsG0880043107.01	0.813398	2.612368e-51	1.704176e-48
MsG0580027955.01	MsG0880043298.01	0.822664	2.099419e-53	1.768574e-50
MsG0580027955.01	MsG0880044209.01	0.801470	8.868520e-49	4.238670e-46
MsG0580027955.01	MsG0880044891.01	0.820890	5.399891e-53	4.326835e-50
MsG0580027955.01	MsG0880044892.01	0.816892	4.374846e-52	3.138702e-49
MsG0580027955.01	MsG0880044893.01	0.830451	2.915550e-55	3.072650e-52
MsG0580027955.01	MsG0880045722.01	0.819727	9.975828e-53	7.739064e-50
MsG0580027955.01	MsG0880045765.01	0.801768	7.705260e-49	3.710434e-46
MsG0580027955.01	MsG0880045992.01	0.823236	1.544206e-53	1.322028e-50
MsG0580027955.01	MsG0880046200.01	0.828603	8.202433e-55	8.192184e-52
MsG0580027955.01	MsG0880046334.01	-0.829207	5.855555e-55	5.953216e-52
MsG0580027955.01	MsG0880046399.01	-0.802394	5.728805e-49	2.803225e-46
MsG0580027955.01	MsG0880046434.01	-0.836712	7.967860e-57	1.012424e-53
MsG0580027955.01	MsG0880046699.01	0.824407	8.209274e-54	7.264316e-51
MsG0580027955.01	MsG0880047481.01	-0.802982	4.332528e-49	2.151790e-46
MsG0280006873.01	MsG0580027955.01	0.833161	6.253297e-56	7.141955e-53
MsG0280006914.01	MsG0580027955.01	0.853953	1.699496e-61	3.735645e-58
MsG0280007647.01	MsG0580027955.01	0.827216	1.768163e-54	1.696010e-51
MsG0280007877.01	MsG0580027955.01	0.801235	9.908630e-49	4.707938e-46
MsG0280008098.01	MsG0580027955.01	0.815188	1.050751e-51	7.194483e-49
MsG0280008928.01	MsG0580027955.01	0.813182	2.913312e-51	1.889171e-48
MsG0280008929.01	MsG0580027955.01	0.810238	1.272944e-50	7.632867e-48
MsG0280009256.01	MsG0580027955.01	0.800145	1.653688e-48	7.642892e-46
MsG0280009699.01	MsG0580027955.01	0.809399	1.928803e-50	1.131324e-47
MsG0280009756.01	MsG0580027955.01	0.809287	2.038768e-50	1.192237e-47
MsG0280010216.01	MsG0580027955.01	0.816497	5.364933e-52	3.807398e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027955.01.T01	MTR_5g066180	86.538	52	7	0	1	52	109	160	3.38e-22	85.1
MsG0580027955.01.T01	MTR_5g032520	52.308	65	29	1	1	63	111	175	2.24e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 10 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAGATTCTTAATGAAAT+TGG	0.154424	5:+71761296	MsG0580027955.01.T01:CDS
AGAAGATTCTTAATGAAATT+GGG	0.314719	5:+71761297	MsG0580027955.01.T01:CDS
GCTCATATAAATGCTTTGTT+TGG	0.342180	5:+71761365	MsG0580027955.01.T01:CDS
CTTAGAGGGGTTGCAGCAAT+TGG	0.364033	5:+71760829	MsG0580027955.01.T01:CDS
TTGGAGAAAACTCTTGAATC+AGG	0.405786	5:+71760848	MsG0580027955.01.T01:CDS
AATTGGGGCACTTCAAATGA+AGG	0.459577	5:+71761313	MsG0580027955.01.T01:CDS
CAAGCGTGTGATTGAGATGA+AGG	0.497959	5:+71760874	MsG0580027955.01.T01:CDS
TTTGAAGGGTTAAACTTAGA+GGG	0.500607	5:+71760815	MsG0580027955.01.T01:CDS
GAAGATTCTTAATGAAATTG+GGG	0.550878	5:+71761298	MsG0580027955.01.T01:CDS
TTGAAGGGTTAAACTTAGAG+GGG	0.563668	5:+71760816	MsG0580027955.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATAAACAAATTTGTTCAA+AGG	+	Chr5:71761184-71761203	MsG0580027955.01.T01:intron	15.0%
!!!	TTTTTTAATCTTTTACATTC+AGG	+	Chr5:71761271-71761290	MsG0580027955.01.T01:intron	15.0%
!!	AAGAAGATTCTTAATGAAAT+TGG	+	Chr5:71761296-71761315	MsG0580027955.01.T01:CDS	20.0%
!!	AGAAGATTCTTAATGAAATT+GGG	+	Chr5:71761297-71761316	MsG0580027955.01.T01:CDS	20.0%
!!	ATATGATCATTTGGTTAATT+TGG	+	Chr5:71760905-71760924	MsG0580027955.01.T01:intron	20.0%
!!	CTGTATATAAAAGTTATAAG+TGG	-	Chr5:71761241-71761260	None:intergenic	20.0%
!!!	ATAAAAGTTATAAGTGGTTT+TGG	-	Chr5:71761235-71761254	None:intergenic	20.0%
!	GAAGATTCTTAATGAAATTG+GGG	+	Chr5:71761298-71761317	MsG0580027955.01.T01:CDS	25.0%
	ATAAATCAATAGCACACACA+AGG	-	Chr5:71760938-71760957	None:intergenic	30.0%
	GTATGCACTATATGATCATT+TGG	+	Chr5:71760896-71760915	MsG0580027955.01.T01:intron	30.0%
	TTTGAAGGGTTAAACTTAGA+GGG	+	Chr5:71760815-71760834	MsG0580027955.01.T01:CDS	30.0%
!	GCTCATATAAATGCTTTGTT+TGG	+	Chr5:71761365-71761384	MsG0580027955.01.T01:CDS	30.0%
!!	GCTTTGTTTGGTAAAAATAG+TGG	+	Chr5:71761377-71761396	MsG0580027955.01.T01:CDS	30.0%
!!	TTTTGAAGGGTTAAACTTAG+AGG	+	Chr5:71760814-71760833	MsG0580027955.01.T01:CDS	30.0%
	TTGAAGGGTTAAACTTAGAG+GGG	+	Chr5:71760816-71760835	MsG0580027955.01.T01:CDS	35.0%
	TTGGAGAAAACTCTTGAATC+AGG	+	Chr5:71760848-71760867	MsG0580027955.01.T01:CDS	35.0%
!	ATGAAGAGTGAGGATTTTGA+AGG	+	Chr5:71760800-71760819	MsG0580027955.01.T01:CDS	35.0%
!	TGAAGAGTGAGGATTTTGAA+GGG	+	Chr5:71760801-71760820	MsG0580027955.01.T01:CDS	35.0%
!	TGGAGTAGGTTTTTCACTTT+CGG	-	Chr5:71761215-71761234	None:intergenic	35.0%
!!!	GTTATAAGTGGTTTTGGAGT+AGG	-	Chr5:71761229-71761248	None:intergenic	35.0%
!	AATTGGGGCACTTCAAATGA+AGG	+	Chr5:71761313-71761332	MsG0580027955.01.T01:CDS	40.0%
	CAAGCGTGTGATTGAGATGA+AGG	+	Chr5:71760874-71760893	MsG0580027955.01.T01:CDS	45.0%
	CTTAGAGGGGTTGCAGCAAT+TGG	+	Chr5:71760829-71760848	MsG0580027955.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	71760800	71761403	71760800	ID=MsG0580027955.01;Name=MsG0580027955.01
Chr5	mRNA	71760800	71761403	71760800	ID=MsG0580027955.01.T01;Parent=MsG0580027955.01;Name=MsG0580027955.01.T01;_AED=0.49;_eAED=0.66;_QI=0\|0\|0\|1\|0\|0\|2\|0\|68
Chr5	exon	71760800	71760895	71760800	ID=MsG0580027955.01.T01:exon:23386;Parent=MsG0580027955.01.T01
Chr5	exon	71761293	71761403	71761293	ID=MsG0580027955.01.T01:exon:23387;Parent=MsG0580027955.01.T01
Chr5	CDS	71760800	71760895	71760800	ID=MsG0580027955.01.T01:cds;Parent=MsG0580027955.01.T01
Chr5	CDS	71761293	71761403	71761293	ID=MsG0580027955.01.T01:cds;Parent=MsG0580027955.01.T01

Gene Sequence	>MsG0580027955.01.T01 ATGAAGAGTGAGGATTTTGAAGGGTTAAACTTAGAGGGGTTGCAGCAATTGGAGAAAACTCTTGAATCAGGACTCAAGCGTGTGATTGAGATGAAGGAAAAGAAGATTCTTAATGAAATTGGGGCACTTCAAATGAAGGTTTGTTTATTGTTTGAACTTCATATAAAAGCTCATATAAATGCTTTGTTTGGTAAAAATAGTGGATAA
Protein sequence	>MsG0580027955.01.T01 MKSEDFEGLNLEGLQQLEKTLESGLKRVIEMKEKKILNEIGALQMKVCLLFELHIKAHINALFGKNSG*