AlfalfaGEDB | MsG0280006873.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006873.01

KEGG: -
GO: GO:0006508 GO:0008234
PFAM: PF00112

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006873.01.T01	XP_039687085.1	59.259	189	52	2	11	174	84	272	4.76E-71	226

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006873.01.T01	Q9FJ47	45.802	131	61	4	11	133	90	218	3.21E-27	107

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006873.01.T01	G7ILI4	59.259	189	52	2	11	174	84	272	2.27e-71	226

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048515.01	MsG0280006873.01	0.815363	9.606264e-52	6.609353e-49
MsG0180000649.01	MsG0280006873.01	0.824088	9.751997e-54	8.552147e-51
MsG0180001740.01	MsG0280006873.01	0.807635	4.591352e-50	2.570403e-47
MsG0180001752.01	MsG0280006873.01	0.850724	1.411386e-60	2.790467e-57
MsG0180005953.01	MsG0280006873.01	0.818082	2.359928e-52	1.749136e-49
MsG0280006826.01	MsG0280006873.01	0.872440	3.175473e-67	1.329040e-63
MsG0280006872.01	MsG0280006873.01	0.812443	4.228269e-51	2.688983e-48
MsG0280006873.01	MsG0280006875.01	0.920152	1.835793e-87	6.127369e-83
MsG0280006873.01	MsG0280009624.01	0.805973	1.030825e-49	5.529158e-47
MsG0280006873.01	MsG0380011731.01	0.820998	5.098812e-53	4.098255e-50
MsG0280006873.01	MsG0380012312.01	0.820737	5.855791e-53	4.671836e-50
MsG0280006873.01	MsG0380012564.01	0.829979	3.801819e-55	3.952793e-52
MsG0280006873.01	MsG0380014892.01	0.804483	2.113634e-49	1.091040e-46
MsG0280006873.01	MsG0380014899.01	0.818939	1.508293e-52	1.144930e-49
MsG0280006873.01	MsG0480018677.01	0.846187	2.543118e-59	4.341426e-56
MsG0280006873.01	MsG0480020486.01	0.825075	5.712357e-54	5.153435e-51
MsG0280006873.01	MsG0480020574.01	0.832525	8.993600e-56	1.007819e-52
MsG0280006873.01	MsG0480020575.01	0.802645	5.085985e-49	2.504462e-46
MsG0280006873.01	MsG0480020887.01	0.800346	1.505029e-48	6.991594e-46
MsG0280006873.01	MsG0480022573.01	0.804356	2.247237e-49	1.156243e-46
MsG0280006873.01	MsG0480024030.01	0.816104	6.568220e-52	4.611920e-49
MsG0280006873.01	MsG0580024447.01	0.825055	5.773572e-54	5.205738e-51
MsG0280006873.01	MsG0580027955.01	0.833161	6.253297e-56	7.141955e-53
MsG0280006873.01	MsG0680034013.01	0.821697	3.516984e-53	2.882286e-50
MsG0280006873.01	MsG0680035439.01	0.809731	1.636543e-50	9.684271e-48
MsG0280006873.01	MsG0680035596.01	0.812533	4.042416e-51	2.576762e-48
MsG0280006873.01	MsG0780036621.01	0.806102	9.680116e-50	5.209287e-47
MsG0280006873.01	MsG0780039438.01	0.823431	1.390034e-53	1.196582e-50
MsG0280006873.01	MsG0780041475.01	0.805234	1.473181e-49	7.753068e-47
MsG0280006873.01	MsG0880044218.01	0.801894	7.257959e-49	3.506554e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006873.01.T01	MTR_2g015840	59.259	189	52	2	11	174	84	272	5.76e-75	226
MsG0280006873.01.T01	MTR_2g015850	77.876	113	21	1	11	119	113	225	2.24e-56	181
MsG0280006873.01.T01	MTR_2g015850	96.970	66	2	0	109	174	299	364	2.01e-40	140
MsG0280006873.01.T01	MTR_2g066980	33.750	240	78	8	11	174	85	319	6.23e-29	109
MsG0280006873.01.T01	MTR_5g022560	50.476	105	44	3	11	109	91	193	8.17e-26	101
MsG0280006873.01.T01	MTR_5g022560	63.768	69	25	0	105	173	275	343	4.34e-24	96.7
MsG0280006873.01.T01	MTR_2g090890	46.018	113	49	3	11	117	89	195	2.74e-25	99.8
MsG0280006873.01.T01	MTR_2g090890	51.471	68	32	1	105	172	269	335	1.10e-19	84.7
MsG0280006873.01.T01	MTR_4g107930	50.476	105	44	3	11	109	92	194	3.14e-25	99.8
MsG0280006873.01.T01	MTR_4g107930	61.429	70	27	0	104	173	275	344	1.97e-24	97.4
MsG0280006873.01.T01	MTR_4g079800	62.319	69	26	0	105	173	276	344	6.27e-25	99.0
MsG0280006873.01.T01	MTR_4g079800	49.524	105	44	4	11	109	93	194	2.82e-24	97.1
MsG0280006873.01.T01	MTR_3g102220	44.882	127	62	3	11	129	105	231	6.70e-25	100
MsG0280006873.01.T01	MTR_3g102220	50.769	65	30	2	109	172	297	360	5.28e-17	77.8
MsG0280006873.01.T01	MTR_4g079440	49.533	107	39	4	11	109	96	195	7.40e-25	98.6
MsG0280006873.01.T01	MTR_4g079440	52.857	70	32	1	105	174	277	345	2.06e-19	84.0
MsG0280006873.01.T01	MTR_4g079770	50.476	105	43	4	11	109	93	194	1.05e-24	98.2
MsG0280006873.01.T01	MTR_4g079770	63.768	69	25	0	105	173	276	344	2.01e-24	97.4
MsG0280006873.01.T01	MTR_4g079470	50.476	105	43	4	11	109	93	194	1.30e-24	98.2
MsG0280006873.01.T01	MTR_4g079470	63.768	69	25	0	105	173	276	344	2.30e-24	97.4
MsG0280006873.01.T01	MTR_3g116080	46.400	125	58	4	11	129	97	218	2.74e-24	97.4
MsG0280006873.01.T01	MTR_3g116080	47.826	69	35	1	105	173	280	347	1.05e-15	73.6
MsG0280006873.01.T01	MTR_4g047610	49.524	105	45	3	11	109	90	192	3.79e-24	96.7
MsG0280006873.01.T01	MTR_4g047610	62.319	69	26	0	105	173	274	342	1.19e-23	95.5
MsG0280006873.01.T01	MTR_1g023210	43.704	135	61	4	11	133	101	232	5.72e-24	96.7
MsG0280006873.01.T01	MTR_4g094918	41.732	127	66	3	11	129	85	211	6.24e-24	96.3
MsG0280006873.01.T01	MTR_4g094918	52.857	70	31	2	105	173	273	341	9.21e-18	79.7
MsG0280006873.01.T01	MTR_3g075390	48.113	106	48	3	11	109	89	194	8.00e-24	96.3
MsG0280006873.01.T01	MTR_3g075390	51.471	68	33	0	105	172	276	343	1.88e-20	87.0
MsG0280006873.01.T01	MTR_1g094135	42.857	126	57	4	11	123	106	229	3.04e-23	94.7
MsG0280006873.01.T01	MTR_7g079130	58.824	68	28	0	105	172	29	96	3.36e-23	88.6
MsG0280006873.01.T01	MTR_1g107550	46.457	127	58	4	11	129	101	225	5.37e-23	94.7
MsG0280006873.01.T01	MTR_1g107550	52.308	65	29	2	109	172	291	354	9.45e-16	74.3
MsG0280006873.01.T01	MTR_4g080730	49.524	105	43	3	11	109	91	191	8.06e-23	93.2
MsG0280006873.01.T01	MTR_4g080730	52.113	71	33	1	103	173	270	339	1.82e-18	81.3
MsG0280006873.01.T01	MTR_1g018840	42.520	127	65	3	11	129	105	231	1.00e-22	94.0
MsG0280006873.01.T01	MTR_1g018840	50.769	65	30	2	109	172	297	360	6.31e-16	74.7
MsG0280006873.01.T01	MTR_2g075830	46.729	107	43	4	11	109	90	190	1.23e-22	92.8
MsG0280006873.01.T01	MTR_2g075830	55.072	69	31	0	105	173	272	340	4.94e-15	71.6
MsG0280006873.01.T01	MTR_1g018840	43.511	131	65	4	11	133	105	234	1.23e-22	94.0
MsG0280006873.01.T01	MTR_1g018840	50.769	65	30	2	109	172	297	360	5.72e-16	74.7
MsG0280006873.01.T01	MTR_4g104370	48.113	106	48	3	11	109	89	194	1.76e-22	92.4
MsG0280006873.01.T01	MTR_4g104370	54.412	68	31	0	105	172	275	342	2.90e-21	89.4
MsG0280006873.01.T01	MTR_4g080700	48.571	105	44	3	11	109	91	191	2.05e-22	92.0
MsG0280006873.01.T01	MTR_4g080700	52.113	71	33	1	103	173	270	339	3.94e-18	80.5
MsG0280006873.01.T01	MTR_4g071060	44.628	121	54	3	11	122	98	214	9.31e-22	90.5
MsG0280006873.01.T01	MTR_7g078580	54.286	70	32	0	103	172	31	100	1.05e-21	85.5
MsG0280006873.01.T01	MTR_4g080360	46.667	105	49	4	11	109	88	191	1.35e-21	89.7
MsG0280006873.01.T01	MTR_4g080360	58.824	68	28	0	105	172	273	340	1.90e-20	86.7
MsG0280006873.01.T01	MTR_1g023810	45.872	109	54	2	26	129	26	134	2.66e-21	87.8
MsG0280006873.01.T01	MTR_1g023810	39.259	135	67	5	48	171	131	261	1.30e-20	86.3
MsG0280006873.01.T01	MTR_7g078600	55.714	70	31	0	105	174	269	338	5.44e-21	88.2
MsG0280006873.01.T01	MTR_7g078600	57.143	70	24	2	47	110	122	191	5.92e-19	82.8
MsG0280006873.01.T01	MTR_2g067560	41.176	136	57	5	11	129	67	196	8.78e-21	89.0
MsG0280006873.01.T01	MTR_2g067560	54.688	64	24	3	107	166	260	322	5.03e-13	66.6
MsG0280006873.01.T01	MTR_2g067900	41.026	117	59	2	11	117	67	183	1.56e-20	86.7
MsG0280006873.01.T01	MTR_2g083930	51.389	72	35	0	103	174	273	344	2.90e-20	86.3
MsG0280006873.01.T01	MTR_2g083930	41.284	109	53	4	11	109	86	193	1.46e-16	76.3
MsG0280006873.01.T01	MTR_2g016180	44.660	103	51	2	11	109	104	204	3.44e-20	87.0
MsG0280006873.01.T01	MTR_7g079230	55.714	70	31	0	105	174	267	336	3.46e-20	85.9
MsG0280006873.01.T01	MTR_7g079230	55.072	69	25	2	47	109	120	188	1.18e-15	73.6
MsG0280006873.01.T01	MTR_2g016270	42.857	105	55	2	10	109	85	189	1.11e-19	85.5
MsG0280006873.01.T01	MTR_3g112430	41.270	126	67	3	11	129	87	212	1.71e-19	84.7
MsG0280006873.01.T01	MTR_3g112430	53.846	65	29	1	109	173	278	341	4.37e-17	78.2
MsG0280006873.01.T01	MTR_1g025450	47.253	91	42	3	26	110	32	122	2.33e-18	82.0
MsG0280006873.01.T01	MTR_4g065077	35.606	132	78	3	11	138	105	233	4.50e-18	80.5
MsG0280006873.01.T01	MTR_8g086470	35.606	132	78	3	11	138	105	233	5.50e-18	80.1
MsG0280006873.01.T01	MTR_4g072790	42.718	103	51	2	11	109	98	196	6.03e-18	79.7
MsG0280006873.01.T01	MTR_2g015920	42.353	85	45	1	49	129	142	226	1.45e-17	79.0
MsG0280006873.01.T01	MTR_4g073660	42.708	96	47	2	11	102	97	188	3.09e-17	77.8
MsG0280006873.01.T01	MTR_4g072340	42.708	96	47	2	11	102	98	189	4.05e-17	77.4
MsG0280006873.01.T01	MTR_2g064475	47.500	80	34	3	48	119	135	214	1.20e-15	73.6
MsG0280006873.01.T01	MTR_2g064475	54.167	72	23	4	107	173	285	351	1.52e-12	64.7
MsG0280006873.01.T01	MTR_7g078630	43.925	107	35	5	20	109	746	844	1.36e-15	73.9
MsG0280006873.01.T01	MTR_4g125300	54.412	68	23	4	110	174	287	349	6.49e-15	71.6
MsG0280006873.01.T01	MTR_4g125300	46.053	76	35	2	48	117	133	208	1.01e-13	68.2
MsG0280006873.01.T01	MTR_2g015930	40.698	86	44	3	49	129	143	226	3.31e-12	63.9
MsG0280006873.01.T01	MTR_2g016380	45.763	59	31	1	115	173	78	135	4.31e-11	60.1
MsG0280006873.01.T01	MTR_7g079260	50.000	68	18	2	103	170	11	62	4.97e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006873.01.T01	AT5G45890	45.802	131	61	4	11	133	90	218	3.27e-28	107
MsG0280006873.01.T01	AT5G45890	50.000	78	39	0	97	174	269	346	2.60e-21	89.4
MsG0280006873.01.T01	AT2G34080	48.031	127	58	3	11	129	90	216	1.01e-27	106
MsG0280006873.01.T01	AT2G34080	59.420	69	28	0	105	173	276	344	3.71e-23	94.4
MsG0280006873.01.T01	AT2G27420	48.120	133	58	5	11	133	86	217	3.49e-27	105
MsG0280006873.01.T01	AT2G27420	57.353	68	29	0	105	172	279	346	6.88e-23	93.6
MsG0280006873.01.T01	AT1G29080	48.760	121	54	3	11	123	90	210	6.39e-27	104
MsG0280006873.01.T01	AT1G29080	61.905	63	24	0	111	173	283	345	4.15e-22	91.3
MsG0280006873.01.T01	AT1G29090	49.180	122	53	4	11	123	98	219	1.43e-26	103
MsG0280006873.01.T01	AT1G29090	57.143	63	27	0	111	173	292	354	2.03e-20	87.0
MsG0280006873.01.T01	AT3G49340	48.696	115	51	3	11	117	86	200	2.44e-26	102
MsG0280006873.01.T01	AT3G49340	58.824	68	28	0	105	172	272	339	7.72e-24	95.9
MsG0280006873.01.T01	AT4G16190	42.857	133	59	4	11	129	105	234	2.45e-25	100
MsG0280006873.01.T01	AT3G48350	49.057	106	47	3	11	109	87	192	1.59e-24	97.1
MsG0280006873.01.T01	AT1G20850	45.600	125	61	4	11	129	101	224	1.90e-24	97.8
MsG0280006873.01.T01	AT1G20850	52.174	69	32	1	105	173	286	353	1.98e-17	78.6
MsG0280006873.01.T01	AT3G48340	50.000	108	45	4	11	109	87	194	2.12e-24	97.8
MsG0280006873.01.T01	AT3G48340	60.294	68	26	1	105	172	276	342	3.91e-22	91.7
MsG0280006873.01.T01	AT3G19390	45.038	131	51	4	11	129	94	215	2.33e-24	98.6
MsG0280006873.01.T01	AT3G19390	60.000	65	25	1	109	173	282	345	2.54e-20	87.4
MsG0280006873.01.T01	AT5G43060	51.923	104	42	3	11	109	104	204	2.39e-24	98.6
MsG0280006873.01.T01	AT5G43060	51.429	70	33	1	103	172	284	352	1.54e-18	82.4
MsG0280006873.01.T01	AT3G48350	49.057	106	47	3	11	109	87	192	3.55e-24	97.1
MsG0280006873.01.T01	AT3G48350	55.385	65	29	0	109	173	279	343	1.83e-20	87.0
MsG0280006873.01.T01	AT4G35350	43.651	126	61	4	11	129	101	223	4.16e-24	95.9
MsG0280006873.01.T01	AT1G06260	44.800	125	60	3	11	129	93	214	5.19e-24	96.7
MsG0280006873.01.T01	AT1G06260	59.677	62	24	1	111	172	281	341	6.07e-19	82.8
MsG0280006873.01.T01	AT4G35350	43.651	126	61	4	11	129	101	223	1.04e-23	95.9
MsG0280006873.01.T01	AT4G35350	50.725	69	33	1	105	173	285	352	1.71e-17	79.0
MsG0280006873.01.T01	AT5G50260	48.113	106	48	3	11	109	87	192	2.14e-23	95.1
MsG0280006873.01.T01	AT5G50260	54.412	68	31	0	105	172	274	341	6.69e-21	88.2
MsG0280006873.01.T01	AT4G36880	40.945	127	67	3	11	129	105	231	6.17e-23	94.0
MsG0280006873.01.T01	AT4G36880	53.623	69	30	2	105	172	293	360	1.16e-18	82.0
MsG0280006873.01.T01	AT4G39090	37.879	132	66	3	11	129	101	229	6.20e-23	94.0
MsG0280006873.01.T01	AT4G11310	42.982	114	51	2	20	129	119	222	8.95e-23	93.6
MsG0280006873.01.T01	AT4G11310	55.882	68	29	1	105	172	285	351	5.40e-19	83.2
MsG0280006873.01.T01	AT3G54940	42.969	128	55	5	11	123	101	225	1.53e-22	91.7
MsG0280006873.01.T01	AT4G11320	40.800	125	54	2	9	129	121	229	3.69e-22	91.7
MsG0280006873.01.T01	AT4G11320	55.882	68	29	1	105	172	292	358	3.30e-19	83.6
MsG0280006873.01.T01	AT4G11320	40.800	125	54	2	9	129	121	229	3.69e-22	91.7
MsG0280006873.01.T01	AT4G11320	55.882	68	29	1	105	172	292	358	3.30e-19	83.6
MsG0280006873.01.T01	AT1G47128	39.516	124	68	3	11	129	102	223	3.99e-22	92.4
MsG0280006873.01.T01	AT1G47128	48.571	70	35	1	103	172	283	351	4.24e-18	80.9
MsG0280006873.01.T01	AT3G54940	42.969	128	55	5	11	123	101	225	5.03e-22	91.3
MsG0280006873.01.T01	AT3G19400	47.458	118	50	4	11	122	95	206	5.68e-22	90.1
MsG0280006873.01.T01	AT1G09850	44.355	124	62	3	11	129	83	204	6.70e-22	91.7
MsG0280006873.01.T01	AT1G09850	48.571	70	35	1	103	172	264	332	8.30e-16	74.3
MsG0280006873.01.T01	AT4G23520	50.485	103	43	3	11	108	98	197	6.85e-22	90.9
MsG0280006873.01.T01	AT4G23520	53.125	64	29	1	109	172	286	348	5.63e-18	80.1
MsG0280006873.01.T01	AT2G21430	43.590	117	54	3	11	118	98	211	8.13e-22	90.9
MsG0280006873.01.T01	AT3G19400	47.458	118	50	4	11	122	95	206	1.18e-21	90.5
MsG0280006873.01.T01	AT3G19400	55.072	69	30	1	105	173	281	348	4.49e-19	83.2
MsG0280006873.01.T01	AT3G43960	46.729	107	46	5	11	109	92	195	4.01e-19	83.6
MsG0280006873.01.T01	AT1G29110	52.941	68	31	1	107	174	268	334	5.21e-18	80.1
MsG0280006873.01.T01	AT1G29110	40.000	115	53	6	11	119	89	193	2.09e-14	70.1
MsG0280006873.01.T01	AT5G60360	59.091	66	22	3	109	174	297	357	4.56e-16	74.7
MsG0280006873.01.T01	AT5G60360	39.823	113	57	4	11	117	109	216	2.57e-13	67.0
MsG0280006873.01.T01	AT5G60360	56.061	66	23	2	109	174	297	356	9.99e-15	71.2
MsG0280006873.01.T01	AT5G60360	39.823	113	57	4	11	117	109	216	2.64e-13	67.0
MsG0280006873.01.T01	AT3G45310	54.412	68	23	4	110	174	295	357	3.62e-14	69.3
MsG0280006873.01.T01	AT3G45310	44.737	76	36	2	48	117	141	216	1.37e-12	65.1
MsG0280006873.01.T01	AT5G60360	39.823	113	57	4	11	117	109	216	2.98e-13	67.0
MsG0280006873.01.T01	AT5G60360	65.909	44	14	1	109	152	297	339	2.64e-12	64.3
MsG0280006873.01.T01	AT3G45310	51.471	68	24	3	110	174	295	356	7.70e-13	65.9
MsG0280006873.01.T01	AT3G45310	44.737	76	36	2	48	117	141	216	1.33e-12	65.1

Find 45 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTTGGAATATCATCATTC+AGG	0.284103	2:-7457968	None:intergenic
ATTGGTTGATTAAGAATTCT+TGG	0.312011	2:+7459258	MsG0280006873.01.T01:CDS
GAGTTTATTGCTTCACATAC+TGG	0.326687	2:+7457885	MsG0280006873.01.T01:CDS
AGAATTCTTGGGGTAAAGAT+TGG	0.334902	2:+7459270	MsG0280006873.01.T01:CDS
TTGGTTGATTAAGAATTCTT+GGG	0.374775	2:+7459259	MsG0280006873.01.T01:CDS
GAATTCTTGGGGTAAAGATT+GGG	0.376941	2:+7459271	MsG0280006873.01.T01:CDS
CTCATTATCTGAGCAACAAT+TGG	0.401955	2:+7458874	MsG0280006873.01.T01:CDS
TGTCTCCAATCAAAATTTGT+TGG	0.429280	2:-7457984	None:intergenic
TAGTTAAATCACGAGGATAC+GGG	0.459511	2:-7457857	None:intergenic
GGTAAAGATTGGGGTGAAGA+AGG	0.460104	2:+7459281	MsG0280006873.01.T01:CDS
CTAGTTAAATCACGAGGATA+CGG	0.467336	2:-7457858	None:intergenic
AATATCATCATTCAGGTTGA+AGG	0.470988	2:-7457961	None:intergenic
GCAGTTACTGCAGTTGGTTA+CGG	0.478465	2:+7459212	MsG0280006873.01.T01:CDS
TACTGCAACTGACCTCATCT+TGG	0.480728	2:-7457937	None:intergenic
AACCATGCAGTTACTGCAGT+TGG	0.506721	2:+7459206	MsG0280006873.01.T01:CDS
AGGTTACATGAAGTTGTTGA+GGG	0.508816	2:+7459301	MsG0280006873.01.T01:CDS
GCAATGCCACATTGACCTCC+TGG	0.513337	2:-7459335	None:intergenic
TTGAGGGAAAGTGGTGAACC+AGG	0.520538	2:+7459317	MsG0280006873.01.T01:CDS
AATTCTTGGGGTAAAGATTG+GGG	0.530447	2:+7459272	MsG0280006873.01.T01:CDS
GGTTACGGTGTAAGTGAAGA+TGG	0.532156	2:+7459227	MsG0280006873.01.T01:CDS
GTGAAGATGGAACTAAGTAT+TGG	0.533019	2:+7459240	MsG0280006873.01.T01:CDS
AAGACATACCACAACTGCCT+TGG	0.539526	2:-7458034	None:intergenic
ATGAAGTTGTTGAGGGAAAG+TGG	0.542145	2:+7459308	MsG0280006873.01.T01:CDS
TGCAACTGACCTCATCTTGG+AGG	0.547643	2:-7457934	None:intergenic
GTTAAAGACCAAGGCAGTTG+TGG	0.558254	2:+7458026	MsG0280006873.01.T01:CDS
CAGAACAATGGGTGTGGTGG+AGG	0.560185	2:+7458911	MsG0280006873.01.T01:CDS
ACTGCCTTGGTCTTTAACAT+CGG	0.570004	2:-7458021	None:intergenic
AAGGTTACATGAAGTTGTTG+AGG	0.582698	2:+7459300	MsG0280006873.01.T01:CDS
ACTGCGATGAACAGAACAAT+GGG	0.582764	2:+7458900	MsG0280006873.01.T01:CDS
ATATCATCATTCAGGTTGAA+GGG	0.586071	2:-7457960	None:intergenic
TGGTTGATTAAGAATTCTTG+GGG	0.586376	2:+7459260	MsG0280006873.01.T01:CDS
AAATCACGAGGATACGGGGC+GGG	0.597476	2:-7457852	None:intergenic
GATGTTTATTCAGGAACATG+TGG	0.602261	2:+7459173	MsG0280006873.01.T01:intron
AACCAACTGCAGTAACTGCA+TGG	0.607601	2:-7459208	None:intergenic
AGTTAAATCACGAGGATACG+GGG	0.617730	2:-7457856	None:intergenic
TCATCTTGGAGGACGAAAGT+CGG	0.622867	2:-7457923	None:intergenic
GAACAGAACAATGGGTGTGG+TGG	0.628748	2:+7458908	MsG0280006873.01.T01:CDS
GACTTTCGTCCTCCAAGATG+AGG	0.630901	2:+7457925	MsG0280006873.01.T01:CDS
GGTGAACCAGGAGGTCAATG+TGG	0.664219	2:+7459329	MsG0280006873.01.T01:CDS
GACTGCGATGAACAGAACAA+TGG	0.669619	2:+7458899	MsG0280006873.01.T01:CDS
GTTACCGATGTTAAAGACCA+AGG	0.675798	2:+7458017	MsG0280006873.01.T01:CDS
GATGAACAGAACAATGGGTG+TGG	0.700071	2:+7458905	MsG0280006873.01.T01:CDS
AGGGAAAGTGGTGAACCAGG+AGG	0.728920	2:+7459320	MsG0280006873.01.T01:CDS
TCTTCACTAGTTAAATCACG+AGG	0.744545	2:-7457864	None:intergenic
TAAATCACGAGGATACGGGG+CGG	0.768472	2:-7457853	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAGTTACATTTTAATATTA+AGG	+	Chr2:7458647-7458666	MsG0280006873.01.T01:intron	10.0%
!!!	TTATTTAGATTTTTCTTTTT+TGG	-	Chr2:7458483-7458502	None:intergenic	10.0%
!!!	TTTTGTGATATAAAATAAAA+TGG	-	Chr2:7458240-7458259	None:intergenic	10.0%
!!	AAATATCGAAAAGTTATTTA+AGG	+	Chr2:7458536-7458555	MsG0280006873.01.T01:intron	15.0%
!!	ATTTCATTTGATCATTTAAT+TGG	-	Chr2:7459026-7459045	None:intergenic	15.0%
!!	TGATATAAAATAAAATGGAA+TGG	-	Chr2:7458235-7458254	None:intergenic	15.0%
!!!	TATTATTCAATATGTTTTGT+AGG	+	Chr2:7458794-7458813	MsG0280006873.01.T01:intron	15.0%
!!!	TTATTTTTAATTAGTCATCT+TGG	+	Chr2:7458073-7458092	MsG0280006873.01.T01:intron	15.0%
!!	AAATATATAATACAAACCCA+AGG	+	Chr2:7458952-7458971	MsG0280006873.01.T01:intron	20.0%
!!	GGTTTGTATTATATATTTGA+AGG	-	Chr2:7458950-7458969	None:intergenic	20.0%
!!!	AATAACTTTTCGATATTTGA+TGG	-	Chr2:7458534-7458553	None:intergenic	20.0%
!!!	ATATTCCAACAAATTTTGAT+TGG	+	Chr2:7457979-7457998	MsG0280006873.01.T01:CDS	20.0%
!!!	TTTAGATTTTTCTTTTTTGG+TGG	-	Chr2:7458480-7458499	None:intergenic	20.0%
!	ACAATGCATAAAAATATGCA+AGG	+	Chr2:7458432-7458451	MsG0280006873.01.T01:intron	25.0%
!	ATTGGTTGATTAAGAATTCT+TGG	+	Chr2:7459258-7459277	MsG0280006873.01.T01:CDS	25.0%
!	GATGAATTTCAACATTACAT+GGG	+	Chr2:7459149-7459168	MsG0280006873.01.T01:intron	25.0%
!	TATGCTTCATTAAAAGTTCT+CGG	-	Chr2:7458275-7458294	None:intergenic	25.0%
!	TGATGAATTTCAACATTACA+TGG	+	Chr2:7459148-7459167	MsG0280006873.01.T01:intron	25.0%
!	TGATGTGTTTATATGGTATT+TGG	-	Chr2:7458311-7458330	None:intergenic	25.0%
!	TTGGTTGATTAAGAATTCTT+GGG	+	Chr2:7459259-7459278	MsG0280006873.01.T01:CDS	25.0%
	AACAAAGTCTCCAATATGTT+TGG	+	Chr2:7458136-7458155	MsG0280006873.01.T01:intron	30.0%
	ACAGATTATCCATATCAAGA+AGG	+	Chr2:7458988-7459007	MsG0280006873.01.T01:intron	30.0%
	ATAAAAATATGCAAGGTCTG+GGG	+	Chr2:7458439-7458458	MsG0280006873.01.T01:intron	30.0%
	CATAAAAATATGCAAGGTCT+GGG	+	Chr2:7458438-7458457	MsG0280006873.01.T01:intron	30.0%
	TACCGAAAATCCAAACATAT+TGG	-	Chr2:7458149-7458168	None:intergenic	30.0%
	TATTGGAGACTTTGTTAGTT+TGG	-	Chr2:7458132-7458151	None:intergenic	30.0%
	TGGTTGATTAAGAATTCTTG+GGG	+	Chr2:7459260-7459279	MsG0280006873.01.T01:CDS	30.0%
	TGTCTCCAATCAAAATTTGT+TGG	-	Chr2:7457987-7458006	None:intergenic	30.0%
	TTGTTGGAATATCATCATTC+AGG	-	Chr2:7457971-7457990	None:intergenic	30.0%
!	ATGTTTTGTAGGATGTTGTT+GGG	+	Chr2:7458805-7458824	MsG0280006873.01.T01:intron	30.0%
!	CTCCAATATGTTTGGATTTT+CGG	+	Chr2:7458144-7458163	MsG0280006873.01.T01:intron	30.0%
!	GATGTGTTGATGTGTTTATA+TGG	-	Chr2:7458318-7458337	None:intergenic	30.0%
!	TATGTTTTGTAGGATGTTGT+TGG	+	Chr2:7458804-7458823	MsG0280006873.01.T01:intron	30.0%
!	TGTTTGGATTTTCGGTATAA+AGG	+	Chr2:7458152-7458171	MsG0280006873.01.T01:intron	30.0%
!!	AATATCATCATTCAGGTTGA+AGG	-	Chr2:7457964-7457983	None:intergenic	30.0%
!!	ATATCATCATTCAGGTTGAA+GGG	-	Chr2:7457963-7457982	None:intergenic	30.0%
!!	TGTAATTTCTAGCCTTAGAT+AGG	-	Chr2:7458678-7458697	None:intergenic	30.0%
!!!	AATTTTGATTGGAGACAACA+AGG	+	Chr2:7457990-7458009	MsG0280006873.01.T01:CDS	30.0%
!!!	AGATTTTTCTTTTTTGGTGG+TGG	-	Chr2:7458477-7458496	None:intergenic	30.0%
	AAAAGTTCTCGGAGGATATT+TGG	-	Chr2:7458264-7458283	None:intergenic	35.0%
	AAGGTTACATGAAGTTGTTG+AGG	+	Chr2:7459300-7459319	MsG0280006873.01.T01:CDS	35.0%
	AATTCTTGGGGTAAAGATTG+GGG	+	Chr2:7459272-7459291	MsG0280006873.01.T01:CDS	35.0%
	AGAATTCTTGGGGTAAAGAT+TGG	+	Chr2:7459270-7459289	MsG0280006873.01.T01:CDS	35.0%
	AGGTTACATGAAGTTGTTGA+GGG	+	Chr2:7459301-7459320	MsG0280006873.01.T01:CDS	35.0%
	ATTAAGGAACCACCTATCTA+AGG	+	Chr2:7458663-7458682	MsG0280006873.01.T01:intron	35.0%
	CTAGTTAAATCACGAGGATA+CGG	-	Chr2:7457861-7457880	None:intergenic	35.0%
	CTCATTATCTGAGCAACAAT+TGG	+	Chr2:7458874-7458893	MsG0280006873.01.T01:CDS	35.0%
	GAATTCTTGGGGTAAAGATT+GGG	+	Chr2:7459271-7459290	MsG0280006873.01.T01:CDS	35.0%
	GAGTTTATTGCTTCACATAC+TGG	+	Chr2:7457885-7457904	MsG0280006873.01.T01:CDS	35.0%
	GATGTTTATTCAGGAACATG+TGG	+	Chr2:7459173-7459192	MsG0280006873.01.T01:intron	35.0%
	GCATAAAAATATGCAAGGTC+TGG	+	Chr2:7458437-7458456	MsG0280006873.01.T01:intron	35.0%
	GGTATAAAGGTTGAATGACA+TGG	+	Chr2:7458165-7458184	MsG0280006873.01.T01:intron	35.0%
	GTGAAGATGGAACTAAGTAT+TGG	+	Chr2:7459240-7459259	MsG0280006873.01.T01:CDS	35.0%
	TAATTAGTCATCTTGGAGCA+TGG	+	Chr2:7458080-7458099	MsG0280006873.01.T01:intron	35.0%
	TACATGGGAGATGTTTATTC+AGG	+	Chr2:7459164-7459183	MsG0280006873.01.T01:intron	35.0%
	TAGTTAAATCACGAGGATAC+GGG	-	Chr2:7457860-7457879	None:intergenic	35.0%
	TCAAAGACACCTAATTCGAT+CGG	+	Chr2:7458578-7458597	MsG0280006873.01.T01:intron	35.0%
	TCTTCACTAGTTAAATCACG+AGG	-	Chr2:7457867-7457886	None:intergenic	35.0%
	TGTCTCACTAACAATACCTT+GGG	-	Chr2:7458971-7458990	None:intergenic	35.0%
!	TAGCAGTTGTTGTTCATCAT+TGG	-	Chr2:7459085-7459104	None:intergenic	35.0%
!!	AATTTCTAGCCTTAGATAGG+TGG	-	Chr2:7458675-7458694	None:intergenic	35.0%
	ACTGCGATGAACAGAACAAT+GGG	+	Chr2:7458900-7458919	MsG0280006873.01.T01:CDS	40.0%
	AGTTAAATCACGAGGATACG+GGG	-	Chr2:7457859-7457878	None:intergenic	40.0%
	ATGAAGTTGTTGAGGGAAAG+TGG	+	Chr2:7459308-7459327	MsG0280006873.01.T01:CDS	40.0%
	CTGTCTCACTAACAATACCT+TGG	-	Chr2:7458972-7458991	None:intergenic	40.0%
	GCTTCATTAAAAGTTCTCGG+AGG	-	Chr2:7458272-7458291	None:intergenic	40.0%
	GTCTGACTACCTTCTTGATA+TGG	-	Chr2:7459000-7459019	None:intergenic	40.0%
	GTTACCGATGTTAAAGACCA+AGG	+	Chr2:7458017-7458036	MsG0280006873.01.T01:CDS	40.0%
!	ACTGCCTTGGTCTTTAACAT+CGG	-	Chr2:7458024-7458043	None:intergenic	40.0%
!	AGTTGTTGTTCATCATTGGC+AGG	-	Chr2:7459081-7459100	None:intergenic	40.0%
!!!	TTTTCTTTTTTGGTGGTGGC+CGG	-	Chr2:7458473-7458492	None:intergenic	40.0%
	AACCAACTGCAGTAACTGCA+TGG	-	Chr2:7459211-7459230	None:intergenic	45.0%
	AACCATGCAGTTACTGCAGT+TGG	+	Chr2:7459206-7459225	MsG0280006873.01.T01:CDS	45.0%
	AAGACATACCACAACTGCCT+TGG	-	Chr2:7458037-7458056	None:intergenic	45.0%
	ACTGCAATGCCAACTGATAC+TGG	-	Chr2:7459125-7459144	None:intergenic	45.0%
	GACTGCGATGAACAGAACAA+TGG	+	Chr2:7458899-7458918	MsG0280006873.01.T01:CDS	45.0%
	GATGAACAGAACAATGGGTG+TGG	+	Chr2:7458905-7458924	MsG0280006873.01.T01:CDS	45.0%
	GCACAAACACCGATCGAATT+AGG	-	Chr2:7458590-7458609	None:intergenic	45.0%
	GCACAACAACCAGTATCAGT+TGG	+	Chr2:7459113-7459132	MsG0280006873.01.T01:intron	45.0%
	GCAGTTACTGCAGTTGGTTA+CGG	+	Chr2:7459212-7459231	MsG0280006873.01.T01:CDS	45.0%
	GGTAAAGATTGGGGTGAAGA+AGG	+	Chr2:7459281-7459300	MsG0280006873.01.T01:CDS	45.0%
	GGTTACGGTGTAAGTGAAGA+TGG	+	Chr2:7459227-7459246	MsG0280006873.01.T01:CDS	45.0%
	GTATCAGTTGGCATTGCAGT+CGG	+	Chr2:7459125-7459144	MsG0280006873.01.T01:intron	45.0%
	GTTAAAGACCAAGGCAGTTG+TGG	+	Chr2:7458026-7458045	MsG0280006873.01.T01:CDS	45.0%
	TACTGCAACTGACCTCATCT+TGG	-	Chr2:7457940-7457959	None:intergenic	45.0%
	TCATCTTGGAGGACGAAAGT+CGG	-	Chr2:7457926-7457945	None:intergenic	45.0%
!!!	TTTCTTTTTTGGTGGTGGCC+GGG	-	Chr2:7458472-7458491	None:intergenic	45.0%
!!	AATAATAAATATAAACATAT+TGG	+	Chr2:7458409-7458428	MsG0280006873.01.T01:intron	5.0%
	GAACAGAACAATGGGTGTGG+TGG	+	Chr2:7458908-7458927	MsG0280006873.01.T01:CDS	50.0%
	GACTTTCGTCCTCCAAGATG+AGG	+	Chr2:7457925-7457944	MsG0280006873.01.T01:CDS	50.0%
	TAAATCACGAGGATACGGGG+CGG	-	Chr2:7457856-7457875	None:intergenic	50.0%
	TGCAACTGACCTCATCTTGG+AGG	-	Chr2:7457937-7457956	None:intergenic	50.0%
	TTGAGGGAAAGTGGTGAACC+AGG	+	Chr2:7459317-7459336	MsG0280006873.01.T01:CDS	50.0%
!	TGGGTGTGGTGGAGGTAATA+TGG	+	Chr2:7458919-7458938	MsG0280006873.01.T01:intron	50.0%
!!!	TTCTTTTTTGGTGGTGGCCG+GGG	-	Chr2:7458471-7458490	None:intergenic	50.0%
	AAATCACGAGGATACGGGGC+GGG	-	Chr2:7457855-7457874	None:intergenic	55.0%
	AGGGAAAGTGGTGAACCAGG+AGG	+	Chr2:7459320-7459339	MsG0280006873.01.T01:CDS	55.0%
	CAGAACAATGGGTGTGGTGG+AGG	+	Chr2:7458911-7458930	MsG0280006873.01.T01:CDS	55.0%
	GCAATGCCACATTGACCTCC+TGG	-	Chr2:7459338-7459357	None:intergenic	55.0%
	GGTGAACCAGGAGGTCAATG+TGG	+	Chr2:7459329-7459348	MsG0280006873.01.T01:CDS	55.0%
!	AAGGTCTGGGGTTCGAACCC+CGG	+	Chr2:7458451-7458470	MsG0280006873.01.T01:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	7457840	7459385	7457840	ID=MsG0280006873.01;Name=MsG0280006873.01
Chr2	mRNA	7457840	7459385	7457840	ID=MsG0280006873.01.T01;Parent=MsG0280006873.01;Name=MsG0280006873.01.T01;_AED=0.44;_eAED=0.47;_QI=0\|0\|0\|1\|1\|1\|3\|0\|174
Chr2	exon	7457840	7458047	7457840	ID=MsG0280006873.01.T01:exon:7878;Parent=MsG0280006873.01.T01
Chr2	exon	7458816	7458932	7458816	ID=MsG0280006873.01.T01:exon:7879;Parent=MsG0280006873.01.T01
Chr2	exon	7459186	7459385	7459186	ID=MsG0280006873.01.T01:exon:7880;Parent=MsG0280006873.01.T01
Chr2	CDS	7457840	7458047	7457840	ID=MsG0280006873.01.T01:cds;Parent=MsG0280006873.01.T01
Chr2	CDS	7458816	7458932	7458816	ID=MsG0280006873.01.T01:cds;Parent=MsG0280006873.01.T01
Chr2	CDS	7459186	7459385	7459186	ID=MsG0280006873.01.T01:cds;Parent=MsG0280006873.01.T01

Gene Sequence

>MsG0280006873.01.T01

ATGATACTCGTGCCCGCCCCGTATCCTCGTGATTTAACTAGTGAAGAGTTTATTGCTTCACATACTGGACTCAAAGTTTCTGACCGACTTTCGTCCTCCAAGATGAGGTCAGTTGCAGTACCCTTCAACCTGAATGATGATATTCCAACAAATTTTGATTGGAGACAACAAGGAGCTGTTACCGATGTTAAAGACCAAGGCAGTTGTGGATGTTGTTGGGCATTTTCAGTCGTAGCTGCTGTGAAAATCAAGACTGATGAGTTGATCTCATTATCTGAGCAACAATTGGTTGACTGCGATGAACAGAACAATGGGTGTGGTGGAGGAACATGTGGACAATCAATGAACCATGCAGTTACTGCAGTTGGTTACGGTGTAAGTGAAGATGGAACTAAGTATTGGTTGATTAAGAATTCTTGGGGTAAAGATTGGGGTGAAGAAGGTTACATGAAGTTGTTGAGGGAAAGTGGTGAACCAGGAGGTCAATGTGGCATTGCTGCACATGCTTCTTATCCCACCATATAA

Protein sequence

>MsG0280006873.01.T01

MILVPAPYPRDLTSEEFIASHTGLKVSDRLSSSKMRSVAVPFNLNDDIPTNFDWRQQGAVTDVKDQGSCGCCWAFSVVAAVKIKTDELISLSEQQLVDCDEQNNGCGGGTCGQSMNHAVTAVGYGVSEDGTKYWLIKNSWGKDWGEEGYMKLLRESGEPGGQCGIAAHASYPTI*