AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021024.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021024.01.T01 MTR_4g064380 91.781 292 13 1 1 292 1 281 0.0 546
MsG0480021024.01.T01 MTR_2g097520 69.307 303 70 5 1 292 1 291 9.10e-146 411
MsG0480021024.01.T01 MTR_7g062150 51.603 312 120 4 1 292 1 301 3.54e-104 306
MsG0480021024.01.T01 MTR_4g021800 52.778 288 109 4 8 279 9 285 4.68e-101 298
MsG0480021024.01.T01 MTR_4g021825 50.993 302 116 6 12 292 34 324 6.92e-97 288
MsG0480021024.01.T01 MTR_4g021800 55.649 239 90 3 57 279 2 240 2.02e-88 264
MsG0480021024.01.T01 MTR_1g085570 48.084 287 106 5 8 292 12 257 1.05e-87 262
MsG0480021024.01.T01 MTR_1g085570 48.084 287 106 5 8 292 4 249 1.64e-87 261
MsG0480021024.01.T01 MTR_1g085570 47.917 288 106 6 8 292 4 250 7.66e-86 257
MsG0480021024.01.T01 MTR_7g062150 48.963 241 93 3 1 222 1 230 2.11e-73 226
MsG0480021024.01.T01 MTR_4g021800 48.918 231 91 4 8 222 9 228 1.21e-70 218
MsG0480021024.01.T01 MTR_1g085570 46.835 237 86 3 8 244 4 200 1.73e-69 214
MsG0480021024.01.T01 MTR_1g104610 41.219 279 122 8 11 281 5 249 7.60e-66 207
MsG0480021024.01.T01 MTR_1g104610 43.830 235 97 6 11 244 5 205 5.27e-59 189
MsG0480021024.01.T01 MTR_4g064270 48.052 154 26 5 141 292 22 123 7.62e-30 109
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021024.01.T01 AT5G59480 60.000 285 100 3 7 288 7 280 2.17e-119 344
MsG0480021024.01.T01 AT5G59480 60.000 285 100 3 7 288 7 280 2.17e-119 344
MsG0480021024.01.T01 AT5G59480 60.000 285 100 3 7 288 7 280 2.17e-119 344
MsG0480021024.01.T01 AT5G59480 60.000 285 100 3 7 288 7 280 2.17e-119 344
MsG0480021024.01.T01 AT5G59480 60.000 285 99 4 7 288 7 279 2.28e-117 338
MsG0480021024.01.T01 AT5G02230 55.102 294 116 3 1 292 1 280 5.91e-101 297
MsG0480021024.01.T01 AT5G02230 55.102 294 116 3 1 292 1 280 5.91e-101 297
MsG0480021024.01.T01 AT5G02230 55.102 294 116 3 1 292 38 317 2.34e-100 297
MsG0480021024.01.T01 AT5G59490 49.829 293 113 5 6 292 2 266 4.25e-85 256
MsG0480021024.01.T01 AT3G62040 52.720 239 82 4 57 292 2 212 5.63e-82 246
MsG0480021024.01.T01 AT5G59490 50.209 239 91 3 6 244 2 212 1.40e-67 210
MsG0480021024.01.T01 AT2G32150 39.453 256 122 4 16 271 7 229 5.47e-62 197
MsG0480021024.01.T01 AT2G32150 40.435 230 104 4 16 245 7 203 3.90e-53 173
MsG0480021024.01.T01 AT2G32150 40.670 209 91 4 16 224 7 182 1.13e-46 155

Find 85 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACATTGTTTCCAAATTTC+AGG 0.187226 4:+52255689 None:intergenic
ATCACCCCTAGCCTGAAATT+TGG 0.191523 4:-52255700 MsG0480021024.01.T01:three_prime_UTR
AACAAGAGACAATCATATTT+TGG 0.202288 4:+52258410 None:intergenic
GGACAACAATGGCTGGTCTT+AGG 0.241770 4:-52257796 MsG0480021024.01.T01:CDS
TATTCGTTATACAAGACTTA+TGG 0.249582 4:-52257818 MsG0480021024.01.T01:CDS
TGATGCTGTCAGAGGAATTC+AGG 0.289738 4:+52257234 None:intergenic
ACACTTTATCCTTTGAGTTC+TGG 0.296418 4:-52258063 MsG0480021024.01.T01:CDS
TTGCAGACAACTGGTGTCTT+TGG 0.298955 4:+52257131 None:intergenic
TGCAAAATGCCCTTGAGAAC+AGG 0.303805 4:+52257480 None:intergenic
TTGACTACAGCGACTTTCAT+AGG 0.319658 4:-52257651 MsG0480021024.01.T01:intron
TCTGGTTTAAGTAGATTATA+TGG 0.325088 4:+52257504 None:intergenic
TGCATTCTTACTGTTCTTTG+AGG 0.326553 4:+52257059 None:intergenic
CTGTGGTCTGTCCACATTTG+AGG 0.361210 4:-52257992 MsG0480021024.01.T01:CDS
TTATACTGTGTAATTACTTA+AGG 0.365491 4:-52255644 MsG0480021024.01.T01:three_prime_UTR
ATTCGTTATACAAGACTTAT+GGG 0.378858 4:-52257817 MsG0480021024.01.T01:CDS
TGTGGTCTGTCCACATTTGA+GGG 0.384219 4:-52257991 MsG0480021024.01.T01:CDS
CTCGTCCATTGCCAGGCAAT+TGG 0.388849 4:-52257682 MsG0480021024.01.T01:intron
TATAACGAATAGCACAATTC+AGG 0.402517 4:+52257830 None:intergenic
GACAACAATGGCTGGTCTTA+GGG 0.420457 4:-52257795 MsG0480021024.01.T01:intron
TCAAAAGGTTTGCAGACAAC+TGG 0.421037 4:+52257122 None:intergenic
AAATATGATTGTCTCTTGTT+CGG 0.422018 4:-52258407 MsG0480021024.01.T01:intron
CTTCAAGGAAGGCGTTGTTT+TGG 0.430835 4:-52255614 MsG0480021024.01.T01:three_prime_UTR
ATTACACAGTATAAGATATA+CGG 0.434744 4:+52255653 None:intergenic
ACTTAAGGCATTATGCTTCA+AGG 0.435396 4:-52255629 MsG0480021024.01.T01:three_prime_UTR
TATCCACTATGATTTGTTTG+TGG 0.446238 4:-52255835 MsG0480021024.01.T01:three_prime_UTR
GGAAGTACCGTATCAGAATC+AGG 0.448802 4:+52257152 None:intergenic
TTGTTTCCAAATTTCAGGCT+AGG 0.455511 4:+52255694 None:intergenic
ACAGTATAAGATATACGGTT+GGG 0.458504 4:+52255658 None:intergenic
TGTTTCCAAATTTCAGGCTA+GGG 0.460671 4:+52255695 None:intergenic
TGGTGGTGTCTTTAGTATGT+TGG 0.460705 4:-52255879 MsG0480021024.01.T01:three_prime_UTR
TGTGAAGTCAAGATGGAAAA+CGG 0.461158 4:-52258458 MsG0480021024.01.T01:exon
GGAAAACGGGCATAAATTCC+AGG 0.464067 4:-52258444 MsG0480021024.01.T01:CDS
GCATTCTTACTGTTCTTTGA+GGG 0.472107 4:+52257060 None:intergenic
TTTACAGACAGCTAAACGTC+TGG 0.475885 4:-52256701 MsG0480021024.01.T01:CDS
GAGAGGCATTTCCAGAGCTA+TGG 0.475952 4:-52255998 MsG0480021024.01.T01:CDS
GGTACATCCGTGCGCAGTAC+AGG 0.476231 4:-52256056 MsG0480021024.01.T01:CDS
CATTGCCAGGCAATTGGCTA+CGG 0.481868 4:-52257676 MsG0480021024.01.T01:intron
GTGAAGTCAAGATGGAAAAC+GGG 0.482586 4:-52258457 MsG0480021024.01.T01:exon
GAGTATATGCTTCAGAAGCT+TGG 0.484652 4:-52257872 MsG0480021024.01.T01:intron
TCGTCGACTTCCCATAGCTC+TGG 0.485678 4:+52255987 None:intergenic
AGAGAAGAGATAGTGGAGAA+TGG 0.485921 4:+52258778 None:intergenic
TATCCACTATGATTTGTTTG+TGG 0.490184 4:-52255899 MsG0480021024.01.T01:three_prime_UTR
ATAGCGGAGAGAAGAGATAG+TGG 0.492445 4:+52258771 None:intergenic
AGAGGCATTTCCAGAGCTAT+GGG 0.495371 4:-52255997 MsG0480021024.01.T01:CDS
GACCACTTACCGCAACTGTA+TGG 0.497596 4:+52256674 None:intergenic
CGCATGTCCCGTGTCTGCAT+TGG 0.498454 4:+52257319 None:intergenic
ATGCCCTTGAGAACAGGGTC+TGG 0.502133 4:+52257486 None:intergenic
GCAAAATGCCCTTGAGAACA+GGG 0.514850 4:+52257481 None:intergenic
TAAACCAGACCCTGTTCTCA+AGG 0.525046 4:-52257490 MsG0480021024.01.T01:CDS
CCACTATGATTTGTTTGTGG+TGG 0.525435 4:-52255896 MsG0480021024.01.T01:three_prime_UTR
CTCAAATGTGGACAGACCAC+AGG 0.536697 4:+52257993 None:intergenic
ATTTGAAAAGGCTTTCAAGT+TGG 0.546736 4:-52257093 MsG0480021024.01.T01:CDS
AGTAGAGTGCTTAAAAGACT+TGG 0.547877 4:-52257296 MsG0480021024.01.T01:CDS
CACAGTATAAGATATACGGT+TGG 0.562828 4:+52255657 None:intergenic
GATGCTGTCAGAGGAATTCA+GGG 0.563816 4:+52257235 None:intergenic
GTAAAGTTGTGAAGTCAAGA+TGG 0.567106 4:-52258465 MsG0480021024.01.T01:exon
CCACCACAAACAAATCATAG+TGG 0.568100 4:+52255896 None:intergenic
ACTTATGGGACAACAATGGC+TGG 0.571812 4:-52257803 MsG0480021024.01.T01:CDS
GCTTCAGAAGCTTGGAATGG+AGG 0.578253 4:-52257864 MsG0480021024.01.T01:intron
ATCCGTGCGCAGTACAGGAG+TGG 0.578313 4:-52256051 MsG0480021024.01.T01:CDS
CACATTCAAGCCCTCAAATG+TGG 0.581256 4:+52257981 None:intergenic
ACACCACAAACAAATCATAG+TGG 0.583279 4:+52255832 None:intergenic
ATCCACTCCTGTACTGCGCA+CGG 0.590254 4:+52256049 None:intergenic
TATGCTTCAGAAGCTTGGAA+TGG 0.599444 4:-52257867 MsG0480021024.01.T01:intron
GTTTCCAAATTTCAGGCTAG+GGG 0.600658 4:+52255696 None:intergenic
AAGGCATTATGCTTCAAGGA+AGG 0.613013 4:-52255625 MsG0480021024.01.T01:three_prime_UTR
GCTTGAAATCATCAACTACA+AGG 0.613118 4:-52255961 MsG0480021024.01.T01:CDS
TTACAGACAGCTAAACGTCT+GGG 0.621120 4:-52256700 MsG0480021024.01.T01:CDS
GTTTAGCTGTCTGTAAATTG+CGG 0.621197 4:+52256707 None:intergenic
CAGTATAAGATATACGGTTG+GGG 0.624453 4:+52255659 None:intergenic
GGAGCAGAGTAAAGCGATAG+CGG 0.624788 4:+52258755 None:intergenic
AGAAGGGATGATGCTGTCAG+AGG 0.631273 4:+52257226 None:intergenic
ACGCCGTCCTGATTCTGATA+CGG 0.633852 4:-52257159 MsG0480021024.01.T01:CDS
AAACCAGACCCTGTTCTCAA+GGG 0.634734 4:-52257489 MsG0480021024.01.T01:CDS
GCTTAAAAGACTTGGACTCG+AGG 0.655535 4:-52257288 MsG0480021024.01.T01:CDS
CACTTACCGCAACTGTATGG+AGG 0.660444 4:+52256677 None:intergenic
TCTGGGCCTCCATACAGTTG+CGG 0.662336 4:-52256683 MsG0480021024.01.T01:intron
TCAAATGTGGACAGACCACA+GGG 0.663114 4:+52257994 None:intergenic
CAAGACTTATGGGACAACAA+TGG 0.663167 4:-52257807 MsG0480021024.01.T01:CDS
GCTGAAATTCCAGAACTCAA+AGG 0.664777 4:+52258054 None:intergenic
GAGTATTCATAATATCAGAG+AGG 0.717994 4:-52256015 MsG0480021024.01.T01:CDS
GAAAGCAAGAGAGAATAATG+CGG 0.721892 4:+52258734 None:intergenic
GGTAATGATTTACCACCCTG+TGG 0.726383 4:-52258009 MsG0480021024.01.T01:CDS
GTACCGTATCAGAATCAGGA+CGG 0.729331 4:+52257156 None:intergenic
AATGTGGACAGACCACAGGG+TGG 0.809260 4:+52257997 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTATTATTTGGTAAAT+TGG - Chr4:52256432-52256451 MsG0480021024.01.T01:intron 10.0%
!!! TTTAAGAATTAGATTTTAAA+AGG - Chr4:52257886-52257905 MsG0480021024.01.T01:intron 10.0%
!! ATCATAAAAAACAAATACAA+TGG + Chr4:52257829-52257848 None:intergenic 15.0%
!! TAATTATCAAAAACAAATCA+GGG + Chr4:52256287-52256306 None:intergenic 15.0%
!! TTATGATTTGAAAAGAATTA+AGG - Chr4:52257842-52257861 MsG0480021024.01.T01:CDS 15.0%
!!! AAGTTTTGAATGTTAATTAT+TGG - Chr4:52257998-52258017 MsG0480021024.01.T01:CDS 15.0%
!!! ACATTTTTTTTTATGCAAAT+AGG - Chr4:52256654-52256673 MsG0480021024.01.T01:intron 15.0%
!!! TATACTTTTCAAATTATTGT+CGG + Chr4:52256091-52256110 None:intergenic 15.0%
!!! TTAAAAGGATTACTTTTATT+TGG - Chr4:52257901-52257920 MsG0480021024.01.T01:intron 15.0%
!! AAATTGAGGAAAAAAACAAA+AGG + Chr4:52255767-52255786 None:intergenic 20.0%
!! AATAACATTGATACTTGATT+GGG - Chr4:52256973-52256992 MsG0480021024.01.T01:intron 20.0%
!! AATTCACTTTATATAGTACA+TGG + Chr4:52258272-52258291 None:intergenic 20.0%
!! ATTACACAGTATAAGATATA+CGG + Chr4:52258734-52258753 None:intergenic 20.0%
!! ATTCATTGTTATATCATGTA+TGG - Chr4:52256024-52256043 MsG0480021024.01.T01:CDS 20.0%
!! GTAATTATCAAAAACAAATC+AGG + Chr4:52256288-52256307 None:intergenic 20.0%
!! TAATAACATTGATACTTGAT+TGG - Chr4:52256972-52256991 MsG0480021024.01.T01:intron 20.0%
!! TTAACATTCAAAACTTTAAC+AGG + Chr4:52257994-52258013 None:intergenic 20.0%
!! TTATACTGTGTAATTACTTA+AGG - Chr4:52258740-52258759 MsG0480021024.01.T01:five_prime_UTR 20.0%
!! TTATATGTAAAAGATACAGA+AGG + Chr4:52257452-52257471 None:intergenic 20.0%
!! TTTATACTCCAAAAAAAGAT+AGG - Chr4:52257489-52257508 MsG0480021024.01.T01:CDS 20.0%
! AAATATGATTGTCTCTTGTT+CGG - Chr4:52255977-52255996 MsG0480021024.01.T01:CDS 25.0%
! ATTCGTTATACAAGACTTAT+GGG - Chr4:52256567-52256586 MsG0480021024.01.T01:intron 25.0%
! CAAACAGCAAAAAATATTGA+AGG - Chr4:52256354-52256373 MsG0480021024.01.T01:intron 25.0%
! TATTCGTTATACAAGACTTA+TGG - Chr4:52256566-52256585 MsG0480021024.01.T01:intron 25.0%
! TCATTCATCAATAACCATTA+GGG + Chr4:52257735-52257754 None:intergenic 25.0%
! TTCATTCATCAATAACCATT+AGG + Chr4:52257736-52257755 None:intergenic 25.0%
!! AACAAGAGACAATCATATTT+TGG + Chr4:52255977-52255996 None:intergenic 25.0%
!! CAAAGTTGATCTATTAAAGA+GGG + Chr4:52257358-52257377 None:intergenic 25.0%
!! GTTAGGTATTTATTTAGAGT+TGG - Chr4:52256475-52256494 MsG0480021024.01.T01:intron 25.0%
!! TCAAAGTTGATCTATTAAAG+AGG + Chr4:52257359-52257378 None:intergenic 25.0%
!! TCTGGTTTAAGTAGATTATA+TGG + Chr4:52256883-52256902 None:intergenic 25.0%
!! TTCATGAATTTTTGTTGAGA+AGG - Chr4:52255837-52255856 MsG0480021024.01.T01:three_prime_UTR 25.0%
!! TTTTCAAATGCATCTTCAAA+AGG + Chr4:52257280-52257299 None:intergenic 25.0%
!!! AAACTTACCACTTTTCTAAA+AGG + Chr4:52256937-52256956 None:intergenic 25.0%
!!! TTTTGAAGATGCATTTGAAA+AGG - Chr4:52257279-52257298 MsG0480021024.01.T01:CDS 25.0%
AAAGAGAAGAGCTATTAGAA+AGG + Chr4:52257389-52257408 None:intergenic 30.0%
ACAGTATAAGATATACGGTT+GGG + Chr4:52258729-52258748 None:intergenic 30.0%
ACATAACAAGCGAAAAATTG+AGG + Chr4:52255781-52255800 None:intergenic 30.0%
ATAAGTTACATTCAACTGAC+TGG + Chr4:52257966-52257985 None:intergenic 30.0%
ATCCCTTCTAATGAAAAAGT+TGG - Chr4:52257172-52257191 MsG0480021024.01.T01:CDS 30.0%
ATTGGTAAAAAGCTGCAAAA+GGG + Chr4:52257050-52257069 None:intergenic 30.0%
CCACATATTAAAGAGCAATT+CGG - Chr4:52258114-52258133 MsG0480021024.01.T01:intron 30.0%
GAGTATTCATAATATCAGAG+AGG - Chr4:52258369-52258388 MsG0480021024.01.T01:intron 30.0%
TATAACGAATAGCACAATTC+AGG + Chr4:52256557-52256576 None:intergenic 30.0%
TCACATTGTTTCCAAATTTC+AGG + Chr4:52258698-52258717 None:intergenic 30.0%
TGAATTTAACCACATGTGTT+GGG - Chr4:52256119-52256138 MsG0480021024.01.T01:intron 30.0%
TGTTACATGCTAATTTCACT+TGG + Chr4:52256823-52256842 None:intergenic 30.0%
TTACATTCAGTATCCGTATT+CGG + Chr4:52256175-52256194 None:intergenic 30.0%
TTAGTTATCAATTGACAAGC+AGG - Chr4:52257527-52257546 MsG0480021024.01.T01:intron 30.0%
TTGAATTTAACCACATGTGT+TGG - Chr4:52256118-52256137 MsG0480021024.01.T01:intron 30.0%
TTTCTAATAGCTCTTCTCTT+TGG - Chr4:52257388-52257407 MsG0480021024.01.T01:intron 30.0%
TTTGATTGTATAGGAGCAAA+GGG + Chr4:52257942-52257961 None:intergenic 30.0%
! ATTTGAAAAGGCTTTCAAGT+TGG - Chr4:52257291-52257310 MsG0480021024.01.T01:CDS 30.0%
! CGATTCCTTTTTCTTTTGTT+CGG - Chr4:52258026-52258045 MsG0480021024.01.T01:CDS 30.0%
! TGCTTGTCAATTGATAACTA+AGG + Chr4:52257528-52257547 None:intergenic 30.0%
!! TATCCACTATGATTTGTTTG+TGG - Chr4:52258485-52258504 MsG0480021024.01.T01:intron 30.0%
!! TTTTGATTGTATAGGAGCAA+AGG + Chr4:52257943-52257962 None:intergenic 30.0%
!!! CATCATGTCCTATCTTTTTT+TGG + Chr4:52257500-52257519 None:intergenic 30.0%
!!! TGCCAACTTTTTCATTAGAA+GGG + Chr4:52257177-52257196 None:intergenic 30.0%
AACATTGATACTTGATTGGG+AGG - Chr4:52256976-52256995 MsG0480021024.01.T01:intron 35.0%
ACACCACAAACAAATCATAG+TGG + Chr4:52258555-52258574 None:intergenic 35.0%
CACAGTATAAGATATACGGT+TGG + Chr4:52258730-52258749 None:intergenic 35.0%
CAGTATAAGATATACGGTTG+GGG + Chr4:52258728-52258747 None:intergenic 35.0%
CATTGGTAAAAAGCTGCAAA+AGG + Chr4:52257051-52257070 None:intergenic 35.0%
CCGAATTGCTCTTTAATATG+TGG + Chr4:52258117-52258136 None:intergenic 35.0%
CTGAATGTAATCACGTATGT+TGG - Chr4:52256185-52256204 MsG0480021024.01.T01:intron 35.0%
GAAAGCAAGAGAGAATAATG+CGG + Chr4:52255653-52255672 None:intergenic 35.0%
GCATTCTTACTGTTCTTTGA+GGG + Chr4:52257327-52257346 None:intergenic 35.0%
GCTTGAAATCATCAACTACA+AGG - Chr4:52258423-52258442 MsG0480021024.01.T01:CDS 35.0%
GGTTTCCGAACAAAAGAAAA+AGG + Chr4:52258034-52258053 None:intergenic 35.0%
GTAAAGTTGTGAAGTCAAGA+TGG - Chr4:52255919-52255938 MsG0480021024.01.T01:exon 35.0%
GTTTAGCTGTCTGTAAATTG+CGG + Chr4:52257680-52257699 None:intergenic 35.0%
TCAAAATCATGTGTCAGTGT+TGG + Chr4:52256214-52256233 None:intergenic 35.0%
TGAAGTTGAAGCATGAAGTT+AGG - Chr4:52256458-52256477 MsG0480021024.01.T01:intron 35.0%
TGACACCTTAAAAACCTTAC+TGG + Chr4:52258055-52258074 None:intergenic 35.0%
TGCATTCTTACTGTTCTTTG+AGG + Chr4:52257328-52257347 None:intergenic 35.0%
TGTGAAGTCAAGATGGAAAA+CGG - Chr4:52255926-52255945 MsG0480021024.01.T01:exon 35.0%
TGTTTCCAAATTTCAGGCTA+GGG + Chr4:52258692-52258711 None:intergenic 35.0%
TTATATCATGTATGGAGCAC+TGG - Chr4:52256032-52256051 MsG0480021024.01.T01:CDS 35.0%
TTGTTTCCAAATTTCAGGCT+AGG + Chr4:52258693-52258712 None:intergenic 35.0%
! ACACTTTATCCTTTGAGTTC+TGG - Chr4:52256321-52256340 MsG0480021024.01.T01:intron 35.0%
! ACTTAAGGCATTATGCTTCA+AGG - Chr4:52258755-52258774 MsG0480021024.01.T01:five_prime_UTR 35.0%
! AGTAGAGTGCTTAAAAGACT+TGG - Chr4:52257088-52257107 MsG0480021024.01.T01:CDS 35.0%
! GGAAACCAGTAAGGTTTTTA+AGG - Chr4:52258047-52258066 MsG0480021024.01.T01:CDS 35.0%
!! AAGCTTGCCTTTTAGAAAAG+TGG - Chr4:52256927-52256946 MsG0480021024.01.T01:intron 35.0%
!!! CTGACTGGTTTTGATTGTAT+AGG + Chr4:52257951-52257970 None:intergenic 35.0%
!!! CTGCCAACTTTTTCATTAGA+AGG + Chr4:52257178-52257197 None:intergenic 35.0%
!!! GAGACAATCATATTTTGGCT+TGG + Chr4:52255972-52255991 None:intergenic 35.0%
!!! TGTTGAGCAGTTTTGTTCAT+GGG - Chr4:52256851-52256870 MsG0480021024.01.T01:intron 35.0%
CAAGACTTATGGGACAACAA+TGG - Chr4:52256577-52256596 MsG0480021024.01.T01:intron 40.0%
CCACCACAAACAAATCATAG+TGG + Chr4:52258491-52258510 None:intergenic 40.0%
GAGTATATGCTTCAGAAGCT+TGG - Chr4:52256512-52256531 MsG0480021024.01.T01:intron 40.0%
GCTGAAATTCCAGAACTCAA+AGG + Chr4:52256333-52256352 None:intergenic 40.0%
GTATATGATCCCAACACATG+TGG + Chr4:52256131-52256150 None:intergenic 40.0%
GTCATCGAAGAACAACTGAA+AGG + Chr4:52257652-52257671 None:intergenic 40.0%
GTGAAGTCAAGATGGAAAAC+GGG - Chr4:52255927-52255946 MsG0480021024.01.T01:exon 40.0%
GTTTCCAAATTTCAGGCTAG+GGG + Chr4:52258691-52258710 None:intergenic 40.0%
GTTTGTCACCATAACATGTC+TGG + Chr4:52257588-52257607 None:intergenic 40.0%
TATGCTTCAGAAGCTTGGAA+TGG - Chr4:52256517-52256536 MsG0480021024.01.T01:intron 40.0%
TCAAAAGGTTTGCAGACAAC+TGG + Chr4:52257265-52257284 None:intergenic 40.0%
TTACAGACAGCTAAACGTCT+GGG - Chr4:52257684-52257703 MsG0480021024.01.T01:intron 40.0%
TTACTCCTCCAGACATGTTA+TGG - Chr4:52257577-52257596 MsG0480021024.01.T01:intron 40.0%
TTGACTACAGCGACTTTCAT+AGG - Chr4:52256733-52256752 MsG0480021024.01.T01:intron 40.0%
TTTACAGACAGCTAAACGTC+TGG - Chr4:52257683-52257702 MsG0480021024.01.T01:intron 40.0%
! AAGGCATTATGCTTCAAGGA+AGG - Chr4:52258759-52258778 MsG0480021024.01.T01:five_prime_UTR 40.0%
! CTTTTGTTCGGAAACCAGTA+AGG - Chr4:52258038-52258057 MsG0480021024.01.T01:CDS 40.0%
! CTTTTTACCAATGCAGACAC+GGG - Chr4:52257058-52257077 MsG0480021024.01.T01:intron 40.0%
! GCTTTTTACCAATGCAGACA+CGG - Chr4:52257057-52257076 MsG0480021024.01.T01:intron 40.0%
! TGGTGGTGTCTTTAGTATGT+TGG - Chr4:52258505-52258524 MsG0480021024.01.T01:intron 40.0%
!! CCACTATGATTTGTTTGTGG+TGG - Chr4:52258488-52258507 MsG0480021024.01.T01:intron 40.0%
!!! ATTTTGGCTTGGAAACTTCC+TGG + Chr4:52255961-52255980 None:intergenic 40.0%
!!! GTGTTGAGCAGTTTTGTTCA+TGG - Chr4:52256850-52256869 MsG0480021024.01.T01:intron 40.0%
AAACCAGACCCTGTTCTCAA+GGG - Chr4:52256895-52256914 MsG0480021024.01.T01:intron 45.0%
ACTTATGGGACAACAATGGC+TGG - Chr4:52256581-52256600 MsG0480021024.01.T01:intron 45.0%
ATCACCCCTAGCCTGAAATT+TGG - Chr4:52258684-52258703 MsG0480021024.01.T01:intron 45.0%
CACATTCAAGCCCTCAAATG+TGG + Chr4:52256406-52256425 None:intergenic 45.0%
CTCTCTCTCTCTCTCATTTC+TGG - Chr4:52255724-52255743 MsG0480021024.01.T01:three_prime_UTR 45.0%
GATGCTGTCAGAGGAATTCA+GGG + Chr4:52257152-52257171 None:intergenic 45.0%
GCAAAATGCCCTTGAGAACA+GGG + Chr4:52256906-52256925 None:intergenic 45.0%
GCTTAAAAGACTTGGACTCG+AGG - Chr4:52257096-52257115 MsG0480021024.01.T01:CDS 45.0%
GGAAAACGGGCATAAATTCC+AGG - Chr4:52255940-52255959 MsG0480021024.01.T01:CDS 45.0%
GGTAATGATTTACCACCCTG+TGG - Chr4:52256375-52256394 MsG0480021024.01.T01:intron 45.0%
GTACCGTATCAGAATCAGGA+CGG + Chr4:52257231-52257250 None:intergenic 45.0%
TAAACCAGACCCTGTTCTCA+AGG - Chr4:52256894-52256913 MsG0480021024.01.T01:intron 45.0%
TCAAATGTGGACAGACCACA+GGG + Chr4:52256393-52256412 None:intergenic 45.0%
TCTAATTGCCACATCGATGC+AGG - Chr4:52258303-52258322 MsG0480021024.01.T01:intron 45.0%
TGATGCTGTCAGAGGAATTC+AGG + Chr4:52257153-52257172 None:intergenic 45.0%
TGCAAAATGCCCTTGAGAAC+AGG + Chr4:52256907-52256926 None:intergenic 45.0%
TGTCACCATAACATGTCTGG+AGG + Chr4:52257585-52257604 None:intergenic 45.0%
TGTGGTCTGTCCACATTTGA+GGG - Chr4:52256393-52256412 MsG0480021024.01.T01:intron 45.0%
TTGCAGACAACTGGTGTCTT+TGG + Chr4:52257256-52257275 None:intergenic 45.0%
! ATAGCGGAGAGAAGAGATAG+TGG + Chr4:52255616-52255635 None:intergenic 45.0%
! ATTTTCTCTCGTCCATTGCC+AGG - Chr4:52256695-52256714 MsG0480021024.01.T01:CDS 45.0%
!! AGAGGCATTTCCAGAGCTAT+GGG - Chr4:52258387-52258406 MsG0480021024.01.T01:intron 45.0%
!! GACAACAATGGCTGGTCTTA+GGG - Chr4:52256589-52256608 MsG0480021024.01.T01:intron 45.0%
!! GGAAGTACCGTATCAGAATC+AGG + Chr4:52257235-52257254 None:intergenic 45.0%
!!! CTTCAAGGAAGGCGTTGTTT+TGG - Chr4:52258770-52258789 MsG0480021024.01.T01:five_prime_UTR 45.0%
!! AATATAAAAAAAAAAACAAA+AGG + Chr4:52256783-52256802 None:intergenic 5.0%
!! TGTATATAATTATTATTATT+TGG - Chr4:52256424-52256443 MsG0480021024.01.T01:intron 5.0%
ACGCCGTCCTGATTCTGATA+CGG - Chr4:52257225-52257244 MsG0480021024.01.T01:CDS 50.0%
AGAAGGGATGATGCTGTCAG+AGG + Chr4:52257161-52257180 None:intergenic 50.0%
ATTGCCACATCGATGCAGGT+TGG - Chr4:52258307-52258326 MsG0480021024.01.T01:intron 50.0%
CACTTACCGCAACTGTATGG+AGG + Chr4:52257710-52257729 None:intergenic 50.0%
CATTGCCAGGCAATTGGCTA+CGG - Chr4:52256708-52256727 MsG0480021024.01.T01:CDS 50.0%
CTCAAATGTGGACAGACCAC+AGG + Chr4:52256394-52256413 None:intergenic 50.0%
CTCCATACAGTTGCGGTAAG+TGG - Chr4:52257708-52257727 MsG0480021024.01.T01:intron 50.0%
CTGGCACAGACAATAGACAC+CGG - Chr4:52256051-52256070 MsG0480021024.01.T01:CDS 50.0%
CTGTGGTCTGTCCACATTTG+AGG - Chr4:52256392-52256411 MsG0480021024.01.T01:intron 50.0%
GACCACTTACCGCAACTGTA+TGG + Chr4:52257713-52257732 None:intergenic 50.0%
GACGTGTCAGACACCGAATA+CGG - Chr4:52256159-52256178 MsG0480021024.01.T01:intron 50.0%
GCTTCAGAAGCTTGGAATGG+AGG - Chr4:52256520-52256539 MsG0480021024.01.T01:intron 50.0%
GGAGCAGAGTAAAGCGATAG+CGG + Chr4:52255632-52255651 None:intergenic 50.0%
TTGCGGTAAGTGGTCCCTAA+TGG - Chr4:52257718-52257737 MsG0480021024.01.T01:intron 50.0%
! TGTACCAACCTGCATCGATG+TGG + Chr4:52258314-52258333 None:intergenic 50.0%
!! ATTCGGTGTCTGACACGTCT+TGG + Chr4:52256158-52256177 None:intergenic 50.0%
!! GAGAGGCATTTCCAGAGCTA+TGG - Chr4:52258386-52258405 MsG0480021024.01.T01:intron 50.0%
!! GGACAACAATGGCTGGTCTT+AGG - Chr4:52256588-52256607 MsG0480021024.01.T01:intron 50.0%
!! GTCGGTGTCAGTGTCATATC+CGG + Chr4:52256073-52256092 None:intergenic 50.0%
AATGTGGACAGACCACAGGG+TGG + Chr4:52256390-52256409 None:intergenic 55.0%
ATCCACTCCTGTACTGCGCA+CGG + Chr4:52258338-52258357 None:intergenic 55.0%
ATGCCCTTGAGAACAGGGTC+TGG + Chr4:52256901-52256920 None:intergenic 55.0%
CAAAGCCGTAGCCAATTGCC+TGG + Chr4:52256716-52256735 None:intergenic 55.0%
CGTAGCCAATTGCCTGGCAA+TGG + Chr4:52256710-52256729 None:intergenic 55.0%
CTCGTCCATTGCCAGGCAAT+TGG - Chr4:52256702-52256721 MsG0480021024.01.T01:CDS 55.0%
TCTGGGCCTCCATACAGTTG+CGG - Chr4:52257701-52257720 MsG0480021024.01.T01:intron 55.0%
!! TCGTCGACTTCCCATAGCTC+TGG + Chr4:52258400-52258419 None:intergenic 55.0%
ATCCGTGCGCAGTACAGGAG+TGG - Chr4:52258333-52258352 MsG0480021024.01.T01:intron 60.0%
CGCATGTCCCGTGTCTGCAT+TGG + Chr4:52257068-52257087 None:intergenic 60.0%
GGTACATCCGTGCGCAGTAC+AGG - Chr4:52258328-52258347 MsG0480021024.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 52255608 52258798 52255608 ID=MsG0480021024.01;Name=MsG0480021024.01
Chr4 mRNA 52255608 52258798 52255608 ID=MsG0480021024.01.T01;Parent=MsG0480021024.01;Name=MsG0480021024.01.T01;_AED=0.33;_eAED=0.33;_QI=108|0.87|0.77|1|0.75|0.55|9|324|292
Chr4 exon 52255608 52256081 52255608 ID=MsG0480021024.01.T01:exon:28522;Parent=MsG0480021024.01.T01
Chr4 exon 52256684 52256749 52256684 ID=MsG0480021024.01.T01:exon:28521;Parent=MsG0480021024.01.T01
Chr4 exon 52257070 52257348 52257070 ID=MsG0480021024.01.T01:exon:28520;Parent=MsG0480021024.01.T01
Chr4 exon 52257458 52257545 52257458 ID=MsG0480021024.01.T01:exon:28519;Parent=MsG0480021024.01.T01
Chr4 exon 52257652 52257689 52257652 ID=MsG0480021024.01.T01:exon:28518;Parent=MsG0480021024.01.T01
Chr4 exon 52257796 52257879 52257796 ID=MsG0480021024.01.T01:exon:28517;Parent=MsG0480021024.01.T01
Chr4 exon 52257984 52258096 52257984 ID=MsG0480021024.01.T01:exon:28516;Parent=MsG0480021024.01.T01
Chr4 exon 52258408 52258491 52258408 ID=MsG0480021024.01.T01:exon:28515;Parent=MsG0480021024.01.T01
Chr4 exon 52258714 52258798 52258714 ID=MsG0480021024.01.T01:exon:28514;Parent=MsG0480021024.01.T01
Chr4 five_prime_UTR 52258714 52258798 52258714 ID=MsG0480021024.01.T01:five_prime_utr;Parent=MsG0480021024.01.T01
Chr4 five_prime_UTR 52258469 52258491 52258469 ID=MsG0480021024.01.T01:five_prime_utr;Parent=MsG0480021024.01.T01
Chr4 CDS 52258408 52258468 52258408 ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4 CDS 52257984 52258096 52257984 ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4 CDS 52257796 52257879 52257796 ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4 CDS 52257652 52257689 52257652 ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4 CDS 52257458 52257545 52257458 ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4 CDS 52257070 52257348 52257070 ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4 CDS 52256684 52256749 52256684 ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4 CDS 52255932 52256081 52255932 ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4 three_prime_UTR 52255608 52255931 52255608 ID=MsG0480021024.01.T01:three_prime_utr;Parent=MsG0480021024.01.T01
Gene Sequence

>MsG0480021024.01.T01

ATGGAAAACGGGCATAAATTCCAGGAAGTTTCCAAGCCAAAATATGATTGTCTCTTGTTCGATATTGATGATACACTTTATCCTTTGAGTTCTGGAATTTCAGCGCAAACAGCAAAAAATATTGAAGGTAATGATTTACCACCCTGTGGTCTGTCCACATTTGAGGGCTTGAATAAGCTTGGAATGGAGGCTGCTACAGTTCCTGAATTGTGCTATTCGTTATACAAGACTTATGGGACAACAATGGCTGGTCTTAGGGCAATTGGCTACGGCTTTGACTACAGCGACTTTCATAGTTTTGTTCATGGGAGATTGCCATATAATCTACTTAAACCAGACCCTGTTCTCAAGGGCATTTTGCAAAGCTTGCCTTTTAGAAAAGTGCTTTTTACCAATGCAGACACGGGACATGCGAGTAGAGTGCTTAAAAGACTTGGACTCGAGGATTGTTTTGAAAGAGTTATTAGCTTTGATACCCTGAATTCCTCTGACAGCATCATCCCTTCTAATGAAAAAGTTGGCAGTGAAATTTTTGATTTCTGTGAGTACACACGCCGTCCTGATTCTGATACGGTACTTCCAAAGACACCAGTTGTCTGCAAACCTTTTGAAGATGCATTTGAAAAGGCTTTCAAGTTGGCTGACATTGACCCTCAAAGAACATTGTTCTTCGATGACAGTCTCCGCAATTTACAGACAGCTAAACGTCTGGGCCTCCATACAGTTGCGGTTGGTACATCCGTGCGCAGTACAGGAGTGGATCATGCATTAGAGAGTATTCATAATATCAGAGAGGCATTTCCAGAGCTATGGGAAGTCGACGAGAAGCTTGAAATCATCAACTACAAGGTTGCAATTGAAACAACAGTAAAAGCATAG
Protein sequence

>MsG0480021024.01.T01

MENGHKFQEVSKPKYDCLLFDIDDTLYPLSSGISAQTAKNIEGNDLPPCGLSTFEGLNKLGMEAATVPELCYSLYKTYGTTMAGLRAIGYGFDYSDFHSFVHGRLPYNLLKPDPVLKGILQSLPFRKVLFTNADTGHASRVLKRLGLEDCFERVISFDTLNSSDSIIPSNEKVGSEIFDFCEYTRRPDSDTVLPKTPVVCKPFEDAFEKAFKLADIDPQRTLFFDDSLRNLQTAKRLGLHTVAVGTSVRSTGVDHALESIHNIREAFPELWEVDEKLEIINYKVAIETTVKA*