AlfalfaGEDB | MsG0480021024.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021024.01

KEGG: K07025
GO: GO:0008152 GO:0016787
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021024.01.T01	XP_003606690.1	91.781	292	13	1	1	292	1	281	0	546

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021024.01.T01	P53078	31.092	238	112	10	11	241	51	243	1.31E-14	75.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021024.01.T01	G7JUV7	91.781	292	13	1	1	292	1	281	0.0	546

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480021024.01	MsG0480022243.01	0.801323	9.506394e-49	4.526736e-46
MsG0480021024.01	MsG0580024597.01	0.850769	1.370552e-60	2.713948e-57
MsG0480021024.01	MsG0580024598.01	0.806302	8.787039e-50	4.752857e-47
MsG0480021024.01	MsG0580024601.01	0.819320	1.235418e-52	9.474970e-50
MsG0480021024.01	MsG0880042035.01	0.805845	1.096704e-49	5.863208e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021024.01.T01	MTR_4g064380	91.781	292	13	1	1	292	1	281	0.0	546
MsG0480021024.01.T01	MTR_2g097520	69.307	303	70	5	1	292	1	291	9.10e-146	411
MsG0480021024.01.T01	MTR_7g062150	51.603	312	120	4	1	292	1	301	3.54e-104	306
MsG0480021024.01.T01	MTR_4g021800	52.778	288	109	4	8	279	9	285	4.68e-101	298
MsG0480021024.01.T01	MTR_4g021825	50.993	302	116	6	12	292	34	324	6.92e-97	288
MsG0480021024.01.T01	MTR_4g021800	55.649	239	90	3	57	279	2	240	2.02e-88	264
MsG0480021024.01.T01	MTR_1g085570	48.084	287	106	5	8	292	12	257	1.05e-87	262
MsG0480021024.01.T01	MTR_1g085570	48.084	287	106	5	8	292	4	249	1.64e-87	261
MsG0480021024.01.T01	MTR_1g085570	47.917	288	106	6	8	292	4	250	7.66e-86	257
MsG0480021024.01.T01	MTR_7g062150	48.963	241	93	3	1	222	1	230	2.11e-73	226

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021024.01.T01	AT5G59480	60.000	285	100	3	7	288	7	280	2.17e-119	344
MsG0480021024.01.T01	AT5G59480	60.000	285	100	3	7	288	7	280	2.17e-119	344
MsG0480021024.01.T01	AT5G59480	60.000	285	100	3	7	288	7	280	2.17e-119	344
MsG0480021024.01.T01	AT5G59480	60.000	285	100	3	7	288	7	280	2.17e-119	344
MsG0480021024.01.T01	AT5G59480	60.000	285	99	4	7	288	7	279	2.28e-117	338
MsG0480021024.01.T01	AT5G02230	55.102	294	116	3	1	292	1	280	5.91e-101	297
MsG0480021024.01.T01	AT5G02230	55.102	294	116	3	1	292	1	280	5.91e-101	297
MsG0480021024.01.T01	AT5G02230	55.102	294	116	3	1	292	38	317	2.34e-100	297
MsG0480021024.01.T01	AT5G59490	49.829	293	113	5	6	292	2	266	4.25e-85	256
MsG0480021024.01.T01	AT3G62040	52.720	239	82	4	57	292	2	212	5.63e-82	246

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Find 85 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAGACCCTGTTCTCAA+GGG	0.634734	4:-52257489	MsG0480021024.01.T01:CDS
AAATATGATTGTCTCTTGTT+CGG	0.422018	4:-52258407	MsG0480021024.01.T01:intron
AACAAGAGACAATCATATTT+TGG	0.202288	4:+52258410	None:intergenic
AAGGCATTATGCTTCAAGGA+AGG	0.613013	4:-52255625	MsG0480021024.01.T01:three_prime_UTR
AATGTGGACAGACCACAGGG+TGG	0.809260	4:+52257997	None:intergenic
ACACCACAAACAAATCATAG+TGG	0.583279	4:+52255832	None:intergenic
ACACTTTATCCTTTGAGTTC+TGG	0.296418	4:-52258063	MsG0480021024.01.T01:CDS
ACAGTATAAGATATACGGTT+GGG	0.458504	4:+52255658	None:intergenic
ACGCCGTCCTGATTCTGATA+CGG	0.633852	4:-52257159	MsG0480021024.01.T01:CDS
ACTTAAGGCATTATGCTTCA+AGG	0.435396	4:-52255629	MsG0480021024.01.T01:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAGAAGAGCTATTAGAA+AGG	+	Chr4:52257389-52257408	None:intergenic	30.0%
ACAGTATAAGATATACGGTT+GGG	+	Chr4:52258729-52258748	None:intergenic	30.0%
ACATAACAAGCGAAAAATTG+AGG	+	Chr4:52255781-52255800	None:intergenic	30.0%
ATAAGTTACATTCAACTGAC+TGG	+	Chr4:52257966-52257985	None:intergenic	30.0%
ATCCCTTCTAATGAAAAAGT+TGG	-	Chr4:52257172-52257191	MsG0480021024.01.T01:CDS	30.0%
ATTGGTAAAAAGCTGCAAAA+GGG	+	Chr4:52257050-52257069	None:intergenic	30.0%
CCACATATTAAAGAGCAATT+CGG	-	Chr4:52258114-52258133	MsG0480021024.01.T01:intron	30.0%
GAGTATTCATAATATCAGAG+AGG	-	Chr4:52258369-52258388	MsG0480021024.01.T01:intron	30.0%
TATAACGAATAGCACAATTC+AGG	+	Chr4:52256557-52256576	None:intergenic	30.0%
TCACATTGTTTCCAAATTTC+AGG	+	Chr4:52258698-52258717	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	52255608	52258798	52255608	ID=MsG0480021024.01;Name=MsG0480021024.01
Chr4	mRNA	52255608	52258798	52255608	ID=MsG0480021024.01.T01;Parent=MsG0480021024.01;Name=MsG0480021024.01.T01;_AED=0.33;_eAED=0.33;_QI=108\|0.87\|0.77\|1\|0.75\|0.55\|9\|324\|292
Chr4	exon	52255608	52256081	52255608	ID=MsG0480021024.01.T01:exon:28522;Parent=MsG0480021024.01.T01
Chr4	exon	52256684	52256749	52256684	ID=MsG0480021024.01.T01:exon:28521;Parent=MsG0480021024.01.T01
Chr4	exon	52257070	52257348	52257070	ID=MsG0480021024.01.T01:exon:28520;Parent=MsG0480021024.01.T01
Chr4	exon	52257458	52257545	52257458	ID=MsG0480021024.01.T01:exon:28519;Parent=MsG0480021024.01.T01
Chr4	exon	52257652	52257689	52257652	ID=MsG0480021024.01.T01:exon:28518;Parent=MsG0480021024.01.T01
Chr4	exon	52257796	52257879	52257796	ID=MsG0480021024.01.T01:exon:28517;Parent=MsG0480021024.01.T01
Chr4	exon	52257984	52258096	52257984	ID=MsG0480021024.01.T01:exon:28516;Parent=MsG0480021024.01.T01
Chr4	exon	52258408	52258491	52258408	ID=MsG0480021024.01.T01:exon:28515;Parent=MsG0480021024.01.T01
Chr4	exon	52258714	52258798	52258714	ID=MsG0480021024.01.T01:exon:28514;Parent=MsG0480021024.01.T01
Chr4	five_prime_UTR	52258714	52258798	52258714	ID=MsG0480021024.01.T01:five_prime_utr;Parent=MsG0480021024.01.T01
Chr4	five_prime_UTR	52258469	52258491	52258469	ID=MsG0480021024.01.T01:five_prime_utr;Parent=MsG0480021024.01.T01
Chr4	CDS	52258408	52258468	52258408	ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4	CDS	52257984	52258096	52257984	ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4	CDS	52257796	52257879	52257796	ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4	CDS	52257652	52257689	52257652	ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4	CDS	52257458	52257545	52257458	ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4	CDS	52257070	52257348	52257070	ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4	CDS	52256684	52256749	52256684	ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4	CDS	52255932	52256081	52255932	ID=MsG0480021024.01.T01:cds;Parent=MsG0480021024.01.T01
Chr4	three_prime_UTR	52255608	52255931	52255608	ID=MsG0480021024.01.T01:three_prime_utr;Parent=MsG0480021024.01.T01

Gene Sequence

>MsG0480021024.01.T01

ATGGAAAACGGGCATAAATTCCAGGAAGTTTCCAAGCCAAAATATGATTGTCTCTTGTTCGATATTGATGATACACTTTATCCTTTGAGTTCTGGAATTTCAGCGCAAACAGCAAAAAATATTGAAGGTAATGATTTACCACCCTGTGGTCTGTCCACATTTGAGGGCTTGAATAAGCTTGGAATGGAGGCTGCTACAGTTCCTGAATTGTGCTATTCGTTATACAAGACTTATGGGACAACAATGGCTGGTCTTAGGGCAATTGGCTACGGCTTTGACTACAGCGACTTTCATAGTTTTGTTCATGGGAGATTGCCATATAATCTACTTAAACCAGACCCTGTTCTCAAGGGCATTTTGCAAAGCTTGCCTTTTAGAAAAGTGCTTTTTACCAATGCAGACACGGGACATGCGAGTAGAGTGCTTAAAAGACTTGGACTCGAGGATTGTTTTGAAAGAGTTATTAGCTTTGATACCCTGAATTCCTCTGACAGCATCATCCCTTCTAATGAAAAAGTTGGCAGTGAAATTTTTGATTTCTGTGAGTACACACGCCGTCCTGATTCTGATACGGTACTTCCAAAGACACCAGTTGTCTGCAAACCTTTTGAAGATGCATTTGAAAAGGCTTTCAAGTTGGCTGACATTGACCCTCAAAGAACATTGTTCTTCGATGACAGTCTCCGCAATTTACAGACAGCTAAACGTCTGGGCCTCCATACAGTTGCGGTTGGTACATCCGTGCGCAGTACAGGAGTGGATCATGCATTAGAGAGTATTCATAATATCAGAGAGGCATTTCCAGAGCTATGGGAAGTCGACGAGAAGCTTGAAATCATCAACTACAAGGTTGCAATTGAAACAACAGTAAAAGCATAG

Protein sequence

>MsG0480021024.01.T01

MENGHKFQEVSKPKYDCLLFDIDDTLYPLSSGISAQTAKNIEGNDLPPCGLSTFEGLNKLGMEAATVPELCYSLYKTYGTTMAGLRAIGYGFDYSDFHSFVHGRLPYNLLKPDPVLKGILQSLPFRKVLFTNADTGHASRVLKRLGLEDCFERVISFDTLNSSDSIIPSNEKVGSEIFDFCEYTRRPDSDTVLPKTPVVCKPFEDAFEKAFKLADIDPQRTLFFDDSLRNLQTAKRLGLHTVAVGTSVRSTGVDHALESIHNIREAFPELWEVDEKLEIINYKVAIETTVKA*