AlfalfaGEDB | MsG0480022243.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022243.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022243.01.T01	XP_003607784.1	91.358	81	7	0	1	81	1	81	9.78E-36	125

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022243.01.T01	G7JHG4	91.358	81	7	0	1	81	1	81	4.67e-36	125

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280011073.01	MsG0480022243.01	0.820221	7.692440e-53	6.050038e-50
MsG0280011287.01	MsG0480022243.01	0.839605	1.433990e-57	1.990387e-54
MsG0380013471.01	MsG0480022243.01	0.806275	8.902155e-50	4.812017e-47
MsG0380014179.01	MsG0480022243.01	0.805913	1.061148e-49	5.683037e-47
MsG0480021024.01	MsG0480022243.01	0.801323	9.506394e-49	4.526736e-46
MsG0480022155.01	MsG0480022243.01	0.815352	9.660607e-52	6.644832e-49
MsG0480022239.01	MsG0480022243.01	0.853533	2.243449e-61	4.862938e-58
MsG0480022240.01	MsG0480022243.01	0.828745	7.580252e-55	7.601222e-52
MsG0480022243.01	MsG0480022245.01	0.930543	1.405032e-93	8.396441e-89
MsG0480022243.01	MsG0480022246.01	0.884101	2.555858e-71	1.664472e-67
MsG0480022243.01	MsG0480022248.01	0.859420	4.188702e-63	1.105609e-59
MsG0480022243.01	MsG0580024597.01	0.899308	2.253543e-77	2.770782e-73
MsG0480022243.01	MsG0580024598.01	0.859994	2.813283e-63	7.574565e-60
MsG0480022243.01	MsG0580024600.01	0.800763	1.237472e-48	5.809488e-46
MsG0480022243.01	MsG0580024601.01	0.877289	7.082596e-69	3.542588e-65
MsG0480022243.01	MsG0680032516.01	0.818515	1.883268e-52	1.412715e-49
MsG0480022243.01	MsG0680035588.01	0.834012	3.833499e-56	4.491426e-53
MsG0480022243.01	MsG0780040794.01	0.819162	1.341974e-52	1.024767e-49
MsG0480022243.01	MsG0880042035.01	0.814235	1.707958e-51	1.139762e-48
MsG0480022243.01	MsG0880042794.01	0.805901	1.067284e-49	5.714090e-47
MsG0480022243.01	MsG0880043829.01	0.819877	9.220872e-53	7.183311e-50
MsG0480022243.01	MsG0880044524.01	0.836497	9.038883e-57	1.141066e-53
MsG0480022243.01	MsG0880044564.01	0.808932	2.429362e-50	1.407090e-47
MsG0480022243.01	MsG0880046877.01	-0.814172	1.763583e-51	1.174834e-48
MsG0380017022.01	MsG0480022243.01	0.840152	1.032531e-57	1.457920e-54
MsG0380017023.01	MsG0480022243.01	0.828160	1.048801e-54	1.034119e-51
MsG0380017791.01	MsG0480022243.01	0.854576	1.122440e-61	2.518284e-58
MsG0380018077.01	MsG0480022243.01	-0.800449	1.434048e-48	6.679042e-46
MsG0280007429.01	MsG0480022243.01	0.814651	1.381909e-51	9.326064e-49
MsG0280007743.01	MsG0480022243.01	-0.807974	3.889036e-50	2.196625e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022243.01.T01	MTR_4g082900	91.358	81	7	0	1	81	1	81	1.18e-39	125
MsG0480022243.01.T01	MTR_4g082883	64.865	74	26	0	8	81	26	99	4.45e-25	89.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 19 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCTGGACCTAACGATATTT+TGG	0.245207	4:+70063549	MsG0480022243.01.T01:CDS
AAAACCAAAGGTGCGGTTAA+TGG	0.283737	4:+70063736	MsG0480022243.01.T01:CDS
TTTGCCAATAGGATCTGGTT+TGG	0.364938	4:-70063716	None:intergenic
TTGCCAATAGGATCTGGTTT+GGG	0.376061	4:-70063715	None:intergenic
AGCACCATTAACCGCACCTT+TGG	0.426753	4:-70063740	None:intergenic
GCACTTGCCAAAATATCGTT+AGG	0.455143	4:-70063556	None:intergenic
AAACCCAAACCAGATCCTAT+TGG	0.473112	4:+70063712	MsG0480022243.01.T01:CDS
TAAACAACAAAATCGATGGC+TGG	0.494913	4:+70063532	None:intergenic
GGTGCTACAAAGTCTGTTGG+AGG	0.564564	4:+70063757	MsG0480022243.01.T01:CDS
ACTGTAGGAAATGCTGCAGC+TGG	0.565617	4:+70063607	MsG0480022243.01.T01:CDS
GCTGGTGTTCATAAGACTGT+AGG	0.573068	4:+70063658	MsG0480022243.01.T01:CDS
AATGGTGCTACAAAGTCTGT+TGG	0.575777	4:+70063754	MsG0480022243.01.T01:CDS
ACTGTGGGAAATGCTGCAGC+TGG	0.580044	4:+70063640	MsG0480022243.01.T01:CDS
GCTTCAGCACAGAAAACTGT+AGG	0.594928	4:+70063592	MsG0480022243.01.T01:CDS
GATCCTATTGGCAAAACCAA+AGG	0.604323	4:+70063724	MsG0480022243.01.T01:CDS
AGCTGGTGCCCAGAAAACTG+TGG	0.654418	4:+70063624	MsG0480022243.01.T01:CDS
TGTAGGAGACTATGCTACCA+TGG	0.655972	4:+70063675	MsG0480022243.01.T01:CDS
TATTGGCAAAACCAAAGGTG+CGG	0.681681	4:+70063729	MsG0480022243.01.T01:CDS
GCTGGTGCCCAGAAAACTGT+GGG	0.683027	4:+70063625	MsG0480022243.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTTTACATAATCAGCAGCCA+TGG	-	Chr4:70063695-70063714	None:intergenic	35.0%
	AAACCCAAACCAGATCCTAT+TGG	+	Chr4:70063712-70063731	MsG0480022243.01.T01:CDS	40.0%
	GATCCTATTGGCAAAACCAA+AGG	+	Chr4:70063724-70063743	MsG0480022243.01.T01:CDS	40.0%
	GCACTTGCCAAAATATCGTT+AGG	-	Chr4:70063559-70063578	None:intergenic	40.0%
	TATTGGCAAAACCAAAGGTG+CGG	+	Chr4:70063729-70063748	MsG0480022243.01.T01:CDS	40.0%
	TTTGCCAATAGGATCTGGTT+TGG	-	Chr4:70063719-70063738	None:intergenic	40.0%
!	AAAACCAAAGGTGCGGTTAA+TGG	+	Chr4:70063736-70063755	MsG0480022243.01.T01:CDS	40.0%
!	AGCATTTCCCACAGTTTTCT+GGG	-	Chr4:70063635-70063654	None:intergenic	40.0%
!	TTGCCAATAGGATCTGGTTT+GGG	-	Chr4:70063718-70063737	None:intergenic	40.0%
!!	AATGGTGCTACAAAGTCTGT+TGG	+	Chr4:70063754-70063773	MsG0480022243.01.T01:CDS	40.0%
!!!	CGATATTTTGGCAAGTGCAA+AGG	+	Chr4:70063561-70063580	MsG0480022243.01.T01:CDS	40.0%
!!!	TTGGTTTTGCCAATAGGATC+TGG	-	Chr4:70063724-70063743	None:intergenic	40.0%
	GCTGGTGTTCATAAGACTGT+AGG	+	Chr4:70063658-70063677	MsG0480022243.01.T01:CDS	45.0%
	GCTTCAGCACAGAAAACTGT+AGG	+	Chr4:70063592-70063611	MsG0480022243.01.T01:CDS	45.0%
	TGTAGGAGACTATGCTACCA+TGG	+	Chr4:70063675-70063694	MsG0480022243.01.T01:CDS	45.0%
!	CAGCATTTCCCACAGTTTTC+TGG	-	Chr4:70063636-70063655	None:intergenic	45.0%
!	GGCTGGACCTAACGATATTT+TGG	+	Chr4:70063549-70063568	MsG0480022243.01.T01:CDS	45.0%
!!!	GCACCTTTGGTTTTGCCAAT+AGG	-	Chr4:70063730-70063749	None:intergenic	45.0%
	ACTGTAGGAAATGCTGCAGC+TGG	+	Chr4:70063607-70063626	MsG0480022243.01.T01:CDS	50.0%
	AGCACCATTAACCGCACCTT+TGG	-	Chr4:70063743-70063762	None:intergenic	50.0%
!	GGTGCTACAAAGTCTGTTGG+AGG	+	Chr4:70063757-70063776	MsG0480022243.01.T01:CDS	50.0%
	ACTGTGGGAAATGCTGCAGC+TGG	+	Chr4:70063640-70063659	MsG0480022243.01.T01:CDS	55.0%
	AGCTGGTGCCCAGAAAACTG+TGG	+	Chr4:70063624-70063643	MsG0480022243.01.T01:CDS	55.0%
	GCTGGTGCCCAGAAAACTGT+GGG	+	Chr4:70063625-70063644	MsG0480022243.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	70063547	70063792	70063547	ID=MsG0480022243.01;Name=MsG0480022243.01
Chr4	mRNA	70063547	70063792	70063547	ID=MsG0480022243.01.T01;Parent=MsG0480022243.01;Name=MsG0480022243.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|81
Chr4	exon	70063547	70063792	70063547	ID=MsG0480022243.01.T01:exon:55582;Parent=MsG0480022243.01.T01
Chr4	CDS	70063547	70063792	70063547	ID=MsG0480022243.01.T01:cds;Parent=MsG0480022243.01.T01

Gene Sequence	>MsG0480022243.01.T01 ATGGCTGGACCTAACGATATTTTGGCAAGTGCAAAGGATGCAGCAGCTTCAGCACAGAAAACTGTAGGAAATGCTGCAGCTGGTGCCCAGAAAACTGTGGGAAATGCTGCAGCTGGTGTTCATAAGACTGTAGGAGACTATGCTACCATGGCTGCTGATTATGTAAAACCCAAACCAGATCCTATTGGCAAAACCAAAGGTGCGGTTAATGGTGCTACAAAGTCTGTTGGAGGCTTATTTAAATGA
Protein sequence	>MsG0480022243.01.T01 MAGPNDILASAKDAAASAQKTVGNAAAGAQKTVGNAAAGVHKTVGDYATMAADYVKPKPDPIGKTKGAVNGATKSVGGLFK*