Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021067.01.T01 | XP_003606781.2 | 96.732 | 153 | 5 | 0 | 1 | 153 | 1 | 153 | 1.17E-103 | 303 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021067.01.T01 | Q8GWP3 | 53.061 | 147 | 56 | 5 | 5 | 145 | 1 | 140 | 4.50E-41 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021067.01.T01 | G7JDP2 | 96.732 | 153 | 5 | 0 | 1 | 153 | 1 | 153 | 5.59e-104 | 303 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003600.01 | MsG0480021067.01 | 0.812763 | 3.598774e-51 | 2.307790e-48 |
| MsG0180006195.01 | MsG0480021067.01 | 0.806499 | 7.986168e-50 | 4.341370e-47 |
| MsG0480021067.01 | MsG0480021091.01 | 0.804268 | 2.343250e-49 | 1.202896e-46 |
| MsG0480021067.01 | MsG0580024535.01 | 0.804310 | 2.296508e-49 | 1.180199e-46 |
| MsG0480021067.01 | MsG0880044028.01 | 0.820271 | 7.491544e-53 | 5.900274e-50 |
| MsG0480021067.01 | MsG0880045801.01 | 0.804995 | 1.652548e-49 | 8.643994e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021067.01.T01 | MTR_4g065660 | 96.732 | 153 | 5 | 0 | 1 | 153 | 1 | 153 | 1.42e-107 | 303 |
| MsG0480021067.01.T01 | MTR_4g064963 | 80.833 | 120 | 22 | 1 | 35 | 153 | 46 | 165 | 4.16e-64 | 194 |
| MsG0480021067.01.T01 | MTR_4g065123 | 74.815 | 135 | 32 | 2 | 9 | 142 | 4 | 137 | 3.99e-58 | 177 |
| MsG0480021067.01.T01 | MTR_7g066070 | 46.154 | 143 | 69 | 4 | 8 | 147 | 2 | 139 | 7.05e-37 | 124 |
| MsG0480021067.01.T01 | MTR_4g019870 | 46.087 | 115 | 61 | 1 | 32 | 145 | 13 | 127 | 1.67e-33 | 115 |
| MsG0480021067.01.T01 | MTR_0027s0220 | 50.427 | 117 | 55 | 2 | 17 | 130 | 12 | 128 | 3.08e-31 | 109 |
| MsG0480021067.01.T01 | MTR_1g015000 | 37.795 | 127 | 58 | 5 | 31 | 136 | 1 | 127 | 6.84e-18 | 75.5 |
| MsG0480021067.01.T01 | MTR_3g105330 | 34.375 | 128 | 60 | 3 | 31 | 134 | 1 | 128 | 6.32e-15 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021067.01.T01 | AT2G26975 | 53.061 | 147 | 56 | 5 | 5 | 145 | 1 | 140 | 4.59e-42 | 137 |
| MsG0480021067.01.T01 | AT5G59030 | 44.643 | 168 | 78 | 4 | 1 | 153 | 1 | 168 | 1.31e-35 | 121 |
| MsG0480021067.01.T01 | AT3G46900 | 49.587 | 121 | 57 | 3 | 36 | 152 | 34 | 154 | 3.46e-34 | 117 |
| MsG0480021067.01.T01 | AT5G59040 | 45.082 | 122 | 66 | 1 | 30 | 150 | 28 | 149 | 3.42e-31 | 109 |
| MsG0480021067.01.T01 | AT2G37925 | 43.860 | 114 | 60 | 2 | 30 | 139 | 28 | 141 | 2.17e-27 | 100 |
| MsG0480021067.01.T01 | AT5G20650 | 37.692 | 130 | 55 | 4 | 31 | 134 | 1 | 130 | 9.78e-16 | 70.1 |
Find 40 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGGTTGGCCTAGCTTATT+TGG | 0.201432 | 4:-52960237 | MsG0480021067.01.T01:CDS |
| TTTCAATGGTGGAGTGTTCT+TGG | 0.286706 | 4:-52960195 | MsG0480021067.01.T01:CDS |
| CATTGATTCTCTTTGACAAT+TGG | 0.313757 | 4:-52960409 | MsG0480021067.01.T01:CDS |
| TGATGCACATGACATTCTTC+TGG | 0.316430 | 4:-52960442 | MsG0480021067.01.T01:CDS |
| CTGTGCTTGGTCATGCTGTT+GGG | 0.361371 | 4:-52960169 | MsG0480021067.01.T01:CDS |
| TTGAACCGGGTTTAATGAAC+TGG | 0.362514 | 4:+52960303 | None:intergenic |
| CCTAGCTTATTTGGTTATGT+TGG | 0.373927 | 4:-52960228 | MsG0480021067.01.T01:CDS |
| CTCTTTGACAATTGGCCTGC+TGG | 0.377320 | 4:-52960401 | MsG0480021067.01.T01:CDS |
| TGAACTAGACCGGCCACAAC+AGG | 0.384941 | 4:+52960278 | None:intergenic |
| GGCCACAACAGGGTTTGAAC+CGG | 0.386419 | 4:+52960289 | None:intergenic |
| GCTGTGCTTGGTCATGCTGT+TGG | 0.390679 | 4:-52960170 | MsG0480021067.01.T01:CDS |
| AATTGGCCTGCTGGTAGTTC+TGG | 0.393131 | 4:-52960392 | MsG0480021067.01.T01:CDS |
| TGAGAGAAACTCGATGAGTA+TGG | 0.396012 | 4:+52960331 | None:intergenic |
| GTGTTCTTGGTTGCTGTGCT+TGG | 0.406869 | 4:-52960182 | MsG0480021067.01.T01:CDS |
| TTGGCTCTCATGTCTTTCAA+TGG | 0.439850 | 4:-52960209 | MsG0480021067.01.T01:CDS |
| ACTCATCATCATAGTGTCAT+TGG | 0.442685 | 4:+52960478 | None:intergenic |
| GATGCACATGACATTCTTCT+GGG | 0.480501 | 4:-52960441 | MsG0480021067.01.T01:CDS |
| AACTGATACGGCATGGATCA+TGG | 0.481429 | 4:-52960545 | None:intergenic |
| GCCACAACAGGGTTTGAACC+GGG | 0.512994 | 4:+52960290 | None:intergenic |
| ATGGATCATGGCATGGATCA+TGG | 0.516176 | 4:-52960533 | MsG0480021067.01.T01:CDS |
| GCTCTCATGTCTTTCAATGG+TGG | 0.527046 | 4:-52960206 | MsG0480021067.01.T01:CDS |
| TAGTTCTGGTAAGTATGTAT+TGG | 0.530240 | 4:-52960378 | MsG0480021067.01.T01:CDS |
| GGTTCAAACCCTGTTGTGGC+CGG | 0.535871 | 4:-52960287 | MsG0480021067.01.T01:CDS |
| TTGGTAGATGATGATGACTT+TGG | 0.542496 | 4:+52960497 | None:intergenic |
| CCAACATAACCAAATAAGCT+AGG | 0.547948 | 4:+52960228 | None:intergenic |
| TCGAAATTCTCATCTTGATG+TGG | 0.556567 | 4:+52960116 | None:intergenic |
| ACCCGGTTCAAACCCTGTTG+TGG | 0.557595 | 4:-52960291 | MsG0480021067.01.T01:CDS |
| CAAAAGTGTCTGAACTAGAC+CGG | 0.563621 | 4:+52960268 | None:intergenic |
| AGCACCCAGTTCATTAAACC+CGG | 0.571087 | 4:-52960308 | MsG0480021067.01.T01:CDS |
| CATGGCGAAAACAAGGATCA+AGG | 0.574469 | 4:+52960355 | None:intergenic |
| TGAACCGGGTTTAATGAACT+GGG | 0.600391 | 4:+52960304 | None:intergenic |
| GTATGGACATGGCGAAAACA+AGG | 0.607210 | 4:+52960348 | None:intergenic |
| ATACGGCATGGATCATGGCA+TGG | 0.631638 | 4:-52960540 | None:intergenic |
| TCATGGCATGGATCATGGAA+TGG | 0.632980 | 4:-52960528 | MsG0480021067.01.T01:CDS |
| TACTTACCAGAACTACCAGC+AGG | 0.638786 | 4:+52960386 | None:intergenic |
| CATGGATCATGGAATGGCAA+TGG | 0.647968 | 4:-52960522 | MsG0480021067.01.T01:CDS |
| AAACTCGATGAGTATGGACA+TGG | 0.667480 | 4:+52960337 | None:intergenic |
| GAACTAGACCGGCCACAACA+GGG | 0.667595 | 4:+52960279 | None:intergenic |
| AATAATTAACAAGATAACGG+AGG | 0.670863 | 4:+52960089 | None:intergenic |
| ATGCACATGACATTCTTCTG+GGG | 0.703114 | 4:-52960440 | MsG0480021067.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATTGATTCTCTTTGACAAT+TGG | - | Chr4:52960218-52960237 | MsG0480021067.01.T01:CDS | 30.0% |
| !! | TAGTTCTGGTAAGTATGTAT+TGG | - | Chr4:52960249-52960268 | MsG0480021067.01.T01:CDS | 30.0% |
| ACTCATCATCATAGTGTCAT+TGG | + | Chr4:52960152-52960171 | None:intergenic | 35.0% | |
| CCAACATAACCAAATAAGCT+AGG | + | Chr4:52960402-52960421 | None:intergenic | 35.0% | |
| CCTAGCTTATTTGGTTATGT+TGG | - | Chr4:52960399-52960418 | MsG0480021067.01.T01:CDS | 35.0% | |
| TCGAAATTCTCATCTTGATG+TGG | + | Chr4:52960514-52960533 | None:intergenic | 35.0% | |
| ! | AGACACTTTTGCATGTTCTT+AGG | - | Chr4:52960371-52960390 | MsG0480021067.01.T01:CDS | 35.0% |
| ! | GACACTTTTGCATGTTCTTA+GGG | - | Chr4:52960372-52960391 | MsG0480021067.01.T01:CDS | 35.0% |
| ! | TTGGTAGATGATGATGACTT+TGG | + | Chr4:52960133-52960152 | None:intergenic | 35.0% |
| !!! | GATGTGGTTTTTTGAAAGCA+CGG | + | Chr4:52960498-52960517 | None:intergenic | 35.0% |
| AAACTCGATGAGTATGGACA+TGG | + | Chr4:52960293-52960312 | None:intergenic | 40.0% | |
| ATGCACATGACATTCTTCTG+GGG | - | Chr4:52960187-52960206 | MsG0480021067.01.T01:CDS | 40.0% | |
| GATGCACATGACATTCTTCT+GGG | - | Chr4:52960186-52960205 | MsG0480021067.01.T01:CDS | 40.0% | |
| TGAACCGGGTTTAATGAACT+GGG | + | Chr4:52960326-52960345 | None:intergenic | 40.0% | |
| TGAGAGAAACTCGATGAGTA+TGG | + | Chr4:52960299-52960318 | None:intergenic | 40.0% | |
| TGATGCACATGACATTCTTC+TGG | - | Chr4:52960185-52960204 | MsG0480021067.01.T01:CDS | 40.0% | |
| TTGAACCGGGTTTAATGAAC+TGG | + | Chr4:52960327-52960346 | None:intergenic | 40.0% | |
| ! | CTTTTGCATGTTCTTAGGGT+TGG | - | Chr4:52960376-52960395 | MsG0480021067.01.T01:CDS | 40.0% |
| ! | TTGGCTCTCATGTCTTTCAA+TGG | - | Chr4:52960418-52960437 | MsG0480021067.01.T01:CDS | 40.0% |
| ! | TTTCAATGGTGGAGTGTTCT+TGG | - | Chr4:52960432-52960451 | MsG0480021067.01.T01:CDS | 40.0% |
| !! | CAAAAGTGTCTGAACTAGAC+CGG | + | Chr4:52960362-52960381 | None:intergenic | 40.0% |
| AGCACCCAGTTCATTAAACC+CGG | - | Chr4:52960319-52960338 | MsG0480021067.01.T01:CDS | 45.0% | |
| ATGGATCATGGCATGGATCA+TGG | - | Chr4:52960094-52960113 | MsG0480021067.01.T01:CDS | 45.0% | |
| CATGGATCATGGAATGGCAA+TGG | - | Chr4:52960105-52960124 | MsG0480021067.01.T01:CDS | 45.0% | |
| CATGGCGAAAACAAGGATCA+AGG | + | Chr4:52960275-52960294 | None:intergenic | 45.0% | |
| GCTCTCATGTCTTTCAATGG+TGG | - | Chr4:52960421-52960440 | MsG0480021067.01.T01:CDS | 45.0% | |
| GTATGGACATGGCGAAAACA+AGG | + | Chr4:52960282-52960301 | None:intergenic | 45.0% | |
| TACTTACCAGAACTACCAGC+AGG | + | Chr4:52960244-52960263 | None:intergenic | 45.0% | |
| TCATGGCATGGATCATGGAA+TGG | - | Chr4:52960099-52960118 | MsG0480021067.01.T01:CDS | 45.0% | |
| !! | TAGGGTTGGCCTAGCTTATT+TGG | - | Chr4:52960390-52960409 | MsG0480021067.01.T01:CDS | 45.0% |
| CTGTGCTTGGTCATGCTGTT+GGG | - | Chr4:52960458-52960477 | MsG0480021067.01.T01:CDS | 50.0% | |
| GTGTTCTTGGTTGCTGTGCT+TGG | - | Chr4:52960445-52960464 | MsG0480021067.01.T01:CDS | 50.0% | |
| ! | AATTGGCCTGCTGGTAGTTC+TGG | - | Chr4:52960235-52960254 | MsG0480021067.01.T01:CDS | 50.0% |
| ! | CTCTTTGACAATTGGCCTGC+TGG | - | Chr4:52960226-52960245 | MsG0480021067.01.T01:CDS | 50.0% |
| ACCCGGTTCAAACCCTGTTG+TGG | - | Chr4:52960336-52960355 | MsG0480021067.01.T01:CDS | 55.0% | |
| GAACTAGACCGGCCACAACA+GGG | + | Chr4:52960351-52960370 | None:intergenic | 55.0% | |
| GCCACAACAGGGTTTGAACC+GGG | + | Chr4:52960340-52960359 | None:intergenic | 55.0% | |
| GCTGTGCTTGGTCATGCTGT+TGG | - | Chr4:52960457-52960476 | MsG0480021067.01.T01:CDS | 55.0% | |
| GGCCACAACAGGGTTTGAAC+CGG | + | Chr4:52960341-52960360 | None:intergenic | 55.0% | |
| GGTTCAAACCCTGTTGTGGC+CGG | - | Chr4:52960340-52960359 | MsG0480021067.01.T01:CDS | 55.0% | |
| TGAACTAGACCGGCCACAAC+AGG | + | Chr4:52960352-52960371 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 52960094 | 52960555 | 52960094 | ID=MsG0480021067.01;Name=MsG0480021067.01 |
| Chr4 | mRNA | 52960094 | 52960555 | 52960094 | ID=MsG0480021067.01.T01;Parent=MsG0480021067.01;Name=MsG0480021067.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|153 |
| Chr4 | exon | 52960094 | 52960555 | 52960094 | ID=MsG0480021067.01.T01:exon:900;Parent=MsG0480021067.01.T01 |
| Chr4 | CDS | 52960094 | 52960555 | 52960094 | ID=MsG0480021067.01.T01:cds;Parent=MsG0480021067.01.T01 |
| Gene Sequence |
| Protein sequence |
