Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003600.01.T01 | XP_003590505.1 | 72.072 | 222 | 32 | 2 | 1 | 192 | 1 | 222 | 1.83E-104 | 309 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003600.01.T01 | P08688 | 34.234 | 222 | 108 | 5 | 6 | 190 | 7 | 227 | 3.49E-35 | 126 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003600.01.T01 | G7IBL7 | 72.072 | 222 | 32 | 2 | 1 | 192 | 1 | 222 | 8.74e-105 | 309 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002603.01 | MsG0180003600.01 | 0.805047 | 1.612038e-49 | 8.443249e-47 |
| MsG0180003600.01 | MsG0180005059.01 | 0.816218 | 6.192548e-52 | 4.361812e-49 |
| MsG0180003600.01 | MsG0180005130.01 | 0.816575 | 5.153796e-52 | 3.665585e-49 |
| MsG0180003600.01 | MsG0180005143.01 | 0.818820 | 1.605530e-52 | 1.214513e-49 |
| MsG0180003600.01 | MsG0280007677.01 | 0.819413 | 1.176612e-52 | 9.048107e-50 |
| MsG0180003600.01 | MsG0280007689.01 | 0.826359 | 2.833331e-54 | 2.651247e-51 |
| MsG0180003600.01 | MsG0280010270.01 | 0.820392 | 7.028703e-53 | 5.554767e-50 |
| MsG0180003600.01 | MsG0380017901.01 | 0.815061 | 1.121054e-51 | 7.649543e-49 |
| MsG0180003600.01 | MsG0480018692.01 | 0.827916 | 1.201074e-54 | 1.175767e-51 |
| MsG0180003600.01 | MsG0480018693.01 | 0.814414 | 1.559868e-51 | 1.046031e-48 |
| MsG0180003600.01 | MsG0480019812.01 | -0.808543 | 2.940856e-50 | 1.686141e-47 |
| MsG0180003600.01 | MsG0480019818.01 | 0.807416 | 5.109910e-50 | 2.844524e-47 |
| MsG0180003600.01 | MsG0480021067.01 | 0.812763 | 3.598774e-51 | 2.307790e-48 |
| MsG0180003600.01 | MsG0480021091.01 | 0.809463 | 1.868907e-50 | 1.098002e-47 |
| MsG0180003600.01 | MsG0580025024.01 | 0.815692 | 8.115927e-52 | 5.634734e-49 |
| MsG0180003600.01 | MsG0580028577.01 | 0.812575 | 3.956419e-51 | 2.524703e-48 |
| MsG0180003600.01 | MsG0680032461.01 | 0.807244 | 5.557894e-50 | 3.080123e-47 |
| MsG0180003600.01 | MsG0680032932.01 | 0.804183 | 2.441041e-49 | 1.250364e-46 |
| MsG0180003600.01 | MsG0680034238.01 | 0.811146 | 8.100259e-51 | 4.975608e-48 |
| MsG0180003600.01 | MsG0680035344.01 | 0.806711 | 7.205373e-50 | 3.938215e-47 |
| MsG0180003600.01 | MsG0780038318.01 | 0.844952 | 5.496284e-59 | 9.018582e-56 |
| MsG0180003600.01 | MsG0880042642.01 | 0.802918 | 4.465308e-49 | 2.214288e-46 |
| MsG0180003600.01 | MsG0880044001.01 | 0.824647 | 7.206670e-54 | 6.422707e-51 |
| MsG0180003600.01 | MsG0880044028.01 | 0.816222 | 6.181267e-52 | 4.354250e-49 |
| MsG0180003600.01 | MsG0880045801.01 | 0.824532 | 7.671269e-54 | 6.813537e-51 |
| MsG0180003600.01 | MsG0880046946.01 | 0.825750 | 3.953586e-54 | 3.635911e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003600.01.T01 | MTR_1g066530 | 72.072 | 222 | 32 | 2 | 1 | 192 | 1 | 222 | 2.21e-108 | 309 |
| MsG0180003600.01.T01 | MTR_6g073540 | 38.696 | 230 | 102 | 3 | 1 | 191 | 1 | 230 | 1.10e-46 | 153 |
| MsG0180003600.01.T01 | MTR_6g072470 | 39.604 | 202 | 86 | 1 | 1 | 166 | 1 | 202 | 2.31e-42 | 141 |
| MsG0180003600.01.T01 | MTR_6g048910 | 36.818 | 220 | 102 | 4 | 6 | 190 | 5 | 222 | 1.33e-40 | 137 |
| MsG0180003600.01.T01 | MTR_6g045077 | 34.545 | 220 | 109 | 3 | 4 | 190 | 3 | 220 | 1.28e-38 | 132 |
| MsG0180003600.01.T01 | MTR_6g048620 | 38.288 | 222 | 100 | 6 | 6 | 190 | 5 | 226 | 2.88e-38 | 131 |
| MsG0180003600.01.T01 | MTR_0695s0030 | 33.182 | 220 | 112 | 4 | 6 | 190 | 65 | 284 | 1.03e-35 | 127 |
| MsG0180003600.01.T01 | MTR_8g038070 | 33.182 | 220 | 112 | 4 | 6 | 190 | 219 | 438 | 4.88e-34 | 125 |
| MsG0180003600.01.T01 | MTR_8g038100 | 33.182 | 220 | 112 | 4 | 6 | 190 | 281 | 500 | 1.18e-33 | 125 |
| MsG0180003600.01.T01 | MTR_7g103990 | 32.287 | 223 | 111 | 8 | 6 | 190 | 7 | 227 | 1.06e-31 | 114 |
| MsG0180003600.01.T01 | MTR_0057s0100 | 31.364 | 220 | 116 | 5 | 6 | 190 | 7 | 226 | 1.93e-30 | 111 |
| MsG0180003600.01.T01 | MTR_0057s0170 | 31.982 | 222 | 112 | 6 | 6 | 190 | 7 | 226 | 2.90e-30 | 111 |
| MsG0180003600.01.T01 | MTR_4g102470 | 40.373 | 161 | 81 | 5 | 6 | 152 | 7 | 166 | 1.29e-29 | 108 |
| MsG0180003600.01.T01 | MTR_6g040290 | 34.392 | 189 | 94 | 5 | 6 | 190 | 67 | 229 | 5.27e-29 | 107 |
| MsG0180003600.01.T01 | MTR_6g048810 | 44.961 | 129 | 67 | 3 | 64 | 190 | 174 | 300 | 5.90e-27 | 104 |
| MsG0180003600.01.T01 | MTR_0807s0020 | 29.911 | 224 | 117 | 6 | 1 | 190 | 1 | 218 | 2.38e-26 | 100 |
| MsG0180003600.01.T01 | MTR_0216s0030 | 30.808 | 198 | 99 | 4 | 4 | 167 | 1 | 194 | 3.11e-25 | 97.4 |
| MsG0180003600.01.T01 | MTR_6g048880 | 39.098 | 133 | 74 | 4 | 62 | 190 | 49 | 178 | 8.31e-25 | 95.5 |
| MsG0180003600.01.T01 | MTR_6g049040 | 33.758 | 157 | 69 | 2 | 1 | 157 | 1 | 122 | 4.10e-24 | 92.8 |
| MsG0180003600.01.T01 | MTR_7g012000 | 30.583 | 206 | 87 | 4 | 6 | 179 | 7 | 188 | 2.68e-22 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCGGTACGGTCTTTGAAAA+TGG | 0.203555 | 1:+65009999 | MsG0180003600.01.T01:CDS |
| TTTAACAACACGATCCTTCT+TGG | 0.279901 | 1:-65009625 | None:intergenic |
| ATTAGATCTAGAAGTTTAAT+AGG | 0.298795 | 1:-65010172 | None:intergenic |
| ACTCATTACTCCATTCGTTC+TGG | 0.346885 | 1:+65009963 | MsG0180003600.01.T01:CDS |
| AATGGTGTTTCATCAAGAAA+TGG | 0.395080 | 1:-65009674 | None:intergenic |
| AACATGGTAGTGATTGGAAC+AGG | 0.421529 | 1:-65009814 | None:intergenic |
| GTTACGTGTTCAAGAAGAAA+AGG | 0.446425 | 1:+65009574 | MsG0180003600.01.T01:CDS |
| CAATGGAACATGGTAGTGAT+TGG | 0.462327 | 1:-65009820 | None:intergenic |
| GAATAAGAAAACATTATGTC+CGG | 0.469058 | 1:-65009892 | None:intergenic |
| TGCAGTTGTGAATTATGCTC+CGG | 0.478704 | 1:+65009599 | MsG0180003600.01.T01:CDS |
| TGATGAAACACCATTTGAAA+AGG | 0.482259 | 1:+65009682 | MsG0180003600.01.T01:CDS |
| GACAATACAATCACCTTCGT+TGG | 0.494467 | 1:-65010102 | None:intergenic |
| AGAAGTTTAATAGGCTCTGA+AGG | 0.499900 | 1:-65010163 | None:intergenic |
| TTTAATAGGCTCTGAAGGGC+AGG | 0.501025 | 1:-65010158 | None:intergenic |
| AACAGGGCCATCGAGTAAAT+CGG | 0.507747 | 1:-65009797 | None:intergenic |
| GAAGGGCAGGCCATTCCTCA+TGG | 0.517980 | 1:-65010145 | None:intergenic |
| ACACGATCCTTCTTGGCTCC+CGG | 0.519674 | 1:-65009618 | None:intergenic |
| TGTCAATGGTAGAGTAAAGT+TGG | 0.520266 | 1:+65010121 | MsG0180003600.01.T01:CDS |
| AGGAAATGAGTATGTTCGTA+TGG | 0.536361 | 1:+65009917 | MsG0180003600.01.T01:CDS |
| GAAGGTGATTGTATTGTCAA+TGG | 0.538827 | 1:+65010107 | MsG0180003600.01.T01:CDS |
| GAATTGACGCTGCAATTAAG+TGG | 0.544957 | 1:+65009868 | MsG0180003600.01.T01:CDS |
| AAGCCGATTCATACTTCCGA+TGG | 0.550807 | 1:+65009648 | MsG0180003600.01.T01:CDS |
| AAACCATCGGAAGTATGAAT+CGG | 0.551974 | 1:-65009651 | None:intergenic |
| TTATGCTCCGGGAGCCAAGA+AGG | 0.560419 | 1:+65009611 | MsG0180003600.01.T01:CDS |
| GCAGTTGTGAATTATGCTCC+GGG | 0.572517 | 1:+65009600 | MsG0180003600.01.T01:CDS |
| GACGCTGCAATTAAGTGGAC+CGG | 0.578299 | 1:+65009873 | MsG0180003600.01.T01:CDS |
| GAAATGCAGTATCAATGACT+TGG | 0.591249 | 1:-65009530 | None:intergenic |
| TAAAGTTGGTCCATGAGGAA+TGG | 0.593058 | 1:+65010135 | MsG0180003600.01.T01:CDS |
| TACCATGTTCCATTGTCTCA+AGG | 0.594139 | 1:+65009828 | MsG0180003600.01.T01:CDS |
| CATCAAGAAATGGAAACCAT+CGG | 0.598273 | 1:-65009664 | None:intergenic |
| CGTATTGATATTTCCAACGA+AGG | 0.604655 | 1:+65010089 | MsG0180003600.01.T01:CDS |
| TCAGATACCGATTTACTCGA+TGG | 0.614544 | 1:+65009790 | MsG0180003600.01.T01:intron |
| GAAGTTTAATAGGCTCTGAA+GGG | 0.628949 | 1:-65010162 | None:intergenic |
| TAGAGTAAAGTTGGTCCATG+AGG | 0.649165 | 1:+65010130 | MsG0180003600.01.T01:CDS |
| TTCCTTGAGACAATGGAACA+TGG | 0.662944 | 1:-65009830 | None:intergenic |
| ACATGGTAGTGATTGGAACA+GGG | 0.671114 | 1:-65009813 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATAATGTTTTCTTATTCAA+AGG | + | Chr1:65009897-65009916 | MsG0180003600.01.T01:CDS | 20.0% |
| ! | GAATAAGAAAACATTATGTC+CGG | - | Chr1:65009895-65009914 | None:intergenic | 25.0% |
| AATGGTGTTTCATCAAGAAA+TGG | - | Chr1:65009677-65009696 | None:intergenic | 30.0% | |
| TGATGAAACACCATTTGAAA+AGG | + | Chr1:65009682-65009701 | MsG0180003600.01.T01:CDS | 30.0% | |
| ! | AATCAAGCTTACGTTTTCAA+AGG | + | Chr1:65010053-65010072 | MsG0180003600.01.T01:CDS | 30.0% |
| ! | AATCGGAGAGAAATCTATTT+TGG | - | Chr1:65009771-65009790 | None:intergenic | 30.0% |
| ! | CAATCTACTTCCTTTTCAAA+TGG | - | Chr1:65009695-65009714 | None:intergenic | 30.0% |
| !! | CAAGGAACGTTTTTAGAAAA+TGG | + | Chr1:65009846-65009865 | MsG0180003600.01.T01:CDS | 30.0% |
| !! | TTTTAAAAGTTTGCTCGGTA+CGG | + | Chr1:65009986-65010005 | MsG0180003600.01.T01:CDS | 30.0% |
| !!! | ACTTTTAAAACCAGAACGAA+TGG | - | Chr1:65009976-65009995 | None:intergenic | 30.0% |
| !!! | TCTGGTTTTAAAAGTTTGCT+CGG | + | Chr1:65009981-65010000 | MsG0180003600.01.T01:CDS | 30.0% |
| AAAAACGTTCCTTGAGACAA+TGG | - | Chr1:65009840-65009859 | None:intergenic | 35.0% | |
| AGGAAATGAGTATGTTCGTA+TGG | + | Chr1:65009917-65009936 | MsG0180003600.01.T01:CDS | 35.0% | |
| CATCAAGAAATGGAAACCAT+CGG | - | Chr1:65009667-65009686 | None:intergenic | 35.0% | |
| GAAATGCAGTATCAATGACT+TGG | - | Chr1:65009533-65009552 | None:intergenic | 35.0% | |
| GAAGGTGATTGTATTGTCAA+TGG | + | Chr1:65010107-65010126 | MsG0180003600.01.T01:CDS | 35.0% | |
| GAGTAAATCGGTATCTGAAT+CGG | - | Chr1:65009788-65009807 | None:intergenic | 35.0% | |
| GTTACGTGTTCAAGAAGAAA+AGG | + | Chr1:65009574-65009593 | MsG0180003600.01.T01:CDS | 35.0% | |
| TGTCAATGGTAGAGTAAAGT+TGG | + | Chr1:65010121-65010140 | MsG0180003600.01.T01:CDS | 35.0% | |
| TTTAACAACACGATCCTTCT+TGG | - | Chr1:65009628-65009647 | None:intergenic | 35.0% | |
| ! | AAACCATCGGAAGTATGAAT+CGG | - | Chr1:65009654-65009673 | None:intergenic | 35.0% |
| ! | AGAAGTTTAATAGGCTCTGA+AGG | - | Chr1:65010166-65010185 | None:intergenic | 35.0% |
| ! | CGTATTGATATTTCCAACGA+AGG | + | Chr1:65010089-65010108 | MsG0180003600.01.T01:CDS | 35.0% |
| !! | GAAGTTTAATAGGCTCTGAA+GGG | - | Chr1:65010165-65010184 | None:intergenic | 35.0% |
| ACTCATTACTCCATTCGTTC+TGG | + | Chr1:65009963-65009982 | MsG0180003600.01.T01:CDS | 40.0% | |
| GAATTGACGCTGCAATTAAG+TGG | + | Chr1:65009868-65009887 | MsG0180003600.01.T01:CDS | 40.0% | |
| GACAATACAATCACCTTCGT+TGG | - | Chr1:65010105-65010124 | None:intergenic | 40.0% | |
| TACCATGTTCCATTGTCTCA+AGG | + | Chr1:65009828-65009847 | MsG0180003600.01.T01:CDS | 40.0% | |
| TGCAGTTGTGAATTATGCTC+CGG | + | Chr1:65009599-65009618 | MsG0180003600.01.T01:CDS | 40.0% | |
| TTCCTTGAGACAATGGAACA+TGG | - | Chr1:65009833-65009852 | None:intergenic | 40.0% | |
| ! | AACATGGTAGTGATTGGAAC+AGG | - | Chr1:65009817-65009836 | None:intergenic | 40.0% |
| ! | ACATGGTAGTGATTGGAACA+GGG | - | Chr1:65009816-65009835 | None:intergenic | 40.0% |
| ! | CAATGGAACATGGTAGTGAT+TGG | - | Chr1:65009823-65009842 | None:intergenic | 40.0% |
| ! | TAAAGTTGGTCCATGAGGAA+TGG | + | Chr1:65010135-65010154 | MsG0180003600.01.T01:CDS | 40.0% |
| ! | TAGAGTAAAGTTGGTCCATG+AGG | + | Chr1:65010130-65010149 | MsG0180003600.01.T01:CDS | 40.0% |
| !! | TCAGATACCGATTTACTCGA+TGG | + | Chr1:65009790-65009809 | MsG0180003600.01.T01:intron | 40.0% |
| AACAGGGCCATCGAGTAAAT+CGG | - | Chr1:65009800-65009819 | None:intergenic | 45.0% | |
| ACCGAAGCAATCATCTTCTC+TGG | + | Chr1:65009734-65009753 | MsG0180003600.01.T01:intron | 45.0% | |
| GCAGTTGTGAATTATGCTCC+GGG | + | Chr1:65009600-65009619 | MsG0180003600.01.T01:CDS | 45.0% | |
| GCCAGAGAAGATGATTGCTT+CGG | - | Chr1:65009738-65009757 | None:intergenic | 45.0% | |
| ! | AAGCCGATTCATACTTCCGA+TGG | + | Chr1:65009648-65009667 | MsG0180003600.01.T01:CDS | 45.0% |
| ! | CTCGGTACGGTCTTTGAAAA+TGG | + | Chr1:65009999-65010018 | MsG0180003600.01.T01:CDS | 45.0% |
| !! | GATTGTGCCTTTGAGACAGA+AGG | + | Chr1:65009710-65009729 | MsG0180003600.01.T01:intron | 45.0% |
| !! | TTTAATAGGCTCTGAAGGGC+AGG | - | Chr1:65010161-65010180 | None:intergenic | 45.0% |
| GACGCTGCAATTAAGTGGAC+CGG | + | Chr1:65009873-65009892 | MsG0180003600.01.T01:CDS | 50.0% | |
| !! | TTCGGTGCCTTCTGTCTCAA+AGG | - | Chr1:65009720-65009739 | None:intergenic | 50.0% |
| ACACGATCCTTCTTGGCTCC+CGG | - | Chr1:65009621-65009640 | None:intergenic | 55.0% | |
| TTATGCTCCGGGAGCCAAGA+AGG | + | Chr1:65009611-65009630 | MsG0180003600.01.T01:CDS | 55.0% | |
| GAAGGGCAGGCCATTCCTCA+TGG | - | Chr1:65010148-65010167 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 65009522 | 65010187 | 65009522 | ID=MsG0180003600.01;Name=MsG0180003600.01 |
| Chr1 | mRNA | 65009522 | 65010187 | 65009522 | ID=MsG0180003600.01.T01;Parent=MsG0180003600.01;Name=MsG0180003600.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|192 |
| Chr1 | exon | 65009522 | 65009706 | 65009522 | ID=MsG0180003600.01.T01:exon:50483;Parent=MsG0180003600.01.T01 |
| Chr1 | exon | 65009794 | 65010187 | 65009794 | ID=MsG0180003600.01.T01:exon:50484;Parent=MsG0180003600.01.T01 |
| Chr1 | CDS | 65009522 | 65009706 | 65009522 | ID=MsG0180003600.01.T01:cds;Parent=MsG0180003600.01.T01 |
| Chr1 | CDS | 65009794 | 65010187 | 65009794 | ID=MsG0180003600.01.T01:cds;Parent=MsG0180003600.01.T01 |
| Gene Sequence |
| Protein sequence |
