AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044028.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044028.01.T01 MTR_8g046000 95.413 218 10 0 1 218 19 236 1.20e-159 441
MsG0880044028.01.T01 MTR_8g045890 66.667 162 54 0 57 218 284 445 3.47e-76 236
MsG0880044028.01.T01 MTR_8g445140 50.820 244 68 4 13 204 59 302 1.12e-75 231
MsG0880044028.01.T01 MTR_8g045890 66.667 162 54 0 57 218 325 486 1.51e-75 236
MsG0880044028.01.T01 MTR_8g045890 66.667 162 54 0 57 218 362 523 3.26e-75 236
MsG0880044028.01.T01 MTR_8g445170 62.500 176 61 2 43 218 227 397 1.02e-74 231
MsG0880044028.01.T01 MTR_8g045880 61.458 192 66 2 35 218 137 328 7.51e-74 227
MsG0880044028.01.T01 MTR_8g045880 61.458 192 66 2 35 218 172 363 2.34e-73 226
MsG0880044028.01.T01 MTR_8g045880 61.458 192 66 2 35 218 174 365 3.53e-73 226
MsG0880044028.01.T01 MTR_1g045860 56.667 210 78 6 13 218 69 269 4.11e-73 223
MsG0880044028.01.T01 MTR_2g081770 49.490 196 91 4 13 204 79 270 1.22e-60 191
MsG0880044028.01.T01 MTR_2g081610 45.918 196 97 3 11 204 71 259 9.70e-60 189
MsG0880044028.01.T01 MTR_2g081590 46.392 194 95 3 13 204 73 259 2.44e-57 182
MsG0880044028.01.T01 MTR_8g044290 61.224 147 57 0 35 181 197 343 6.21e-56 184
MsG0880044028.01.T01 MTR_3g109490 34.956 226 114 8 7 204 101 321 1.27e-34 125
MsG0880044028.01.T01 MTR_1g008460 35.681 213 111 7 12 203 88 295 1.36e-34 125
MsG0880044028.01.T01 MTR_1g008470 38.532 218 101 9 12 203 88 298 9.77e-34 122
MsG0880044028.01.T01 MTR_1g008590 38.073 218 103 9 12 203 112 323 4.85e-33 121
MsG0880044028.01.T01 MTR_0312s0030 38.073 218 103 9 12 203 112 323 8.56e-33 120
MsG0880044028.01.T01 MTR_1g007520 38.073 218 103 9 12 203 112 323 8.56e-33 120
MsG0880044028.01.T01 MTR_1g008560 37.500 216 110 8 12 203 114 328 8.36e-32 118
MsG0880044028.01.T01 MTR_1g008580 43.066 137 71 4 74 203 34 170 1.49e-31 113
MsG0880044028.01.T01 MTR_1g008510 37.963 216 105 9 12 203 114 324 1.85e-31 117
MsG0880044028.01.T01 MTR_1g008530 40.972 144 78 4 67 203 79 222 1.97e-31 114
MsG0880044028.01.T01 MTR_1g008500 35.000 220 109 8 12 203 170 383 5.07e-31 117
MsG0880044028.01.T01 MTR_1g008450 41.481 135 74 4 74 203 177 311 2.35e-26 103
MsG0880044028.01.T01 MTR_3g116270 31.193 218 124 7 13 206 292 507 8.74e-23 95.9
MsG0880044028.01.T01 MTR_3g116320 32.110 218 122 7 13 206 474 689 1.07e-22 95.9
MsG0880044028.01.T01 MTR_3g107830 39.865 148 79 5 67 204 108 255 1.91e-22 92.4
MsG0880044028.01.T01 MTR_4g069680 38.514 148 81 6 67 204 139 286 2.42e-20 87.4
MsG0880044028.01.T01 MTR_3g116410 38.514 148 81 5 67 204 128 275 8.42e-20 85.5
MsG0880044028.01.T01 MTR_4g069570 37.013 154 83 6 65 205 184 336 1.69e-18 82.8
MsG0880044028.01.T01 MTR_3g116380 35.065 154 90 6 61 205 151 303 3.68e-18 81.3
MsG0880044028.01.T01 MTR_4g069550 30.719 153 90 6 67 205 313 463 1.28e-12 66.6
MsG0880044028.01.T01 MTR_5g033350 30.968 155 96 5 57 202 46 198 2.68e-12 63.9
MsG0880044028.01.T01 MTR_4g133840 32.298 161 97 7 52 202 28 186 6.09e-12 62.4
MsG0880044028.01.T01 MTR_5g033340 30.968 155 96 5 57 202 225 377 1.04e-11 63.5
MsG0880044028.01.T01 MTR_4g069520 26.941 219 132 9 13 205 507 723 2.28e-11 63.2
MsG0880044028.01.T01 MTR_4g069520 26.941 219 132 9 13 205 493 709 2.84e-11 62.8
MsG0880044028.01.T01 MTR_4g068420 31.343 134 73 6 78 205 1 121 3.68e-11 58.9
MsG0880044028.01.T01 MTR_5g033330 30.323 155 97 5 57 202 464 616 5.76e-11 61.6
MsG0880044028.01.T01 MTR_6g086530 31.469 143 86 6 74 204 63 205 8.54e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044028.01.T01 AT5G25610 37.946 224 109 9 7 203 170 390 3.21e-32 120
MsG0880044028.01.T01 AT1G70370 32.192 146 87 5 67 202 479 622 5.49e-13 67.8
MsG0880044028.01.T01 AT1G70370 32.192 146 87 5 67 202 479 622 5.49e-13 67.8
MsG0880044028.01.T01 AT1G49320 27.434 226 126 9 12 203 57 278 4.49e-12 64.3

Find 46 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAACTTCATCAAGCAAATT+TGG 0.152897 8:+34546001 None:intergenic
GTTGAATATTGGAAAGCTTT+TGG 0.243886 8:+34546440 None:intergenic
ACGAAAATTAAGTCATATTC+AGG 0.260765 8:-34546238 MsG0880044028.01.T01:CDS
TGTTCGGAATGGTTGAATAT+TGG 0.268442 8:+34546429 None:intergenic
GGCAAATTCCTTCATTGATT+CGG 0.336511 8:+34546276 None:intergenic
AACTTACATGGTTCCATTGT+TGG 0.360469 8:-34546086 MsG0880044028.01.T01:CDS
GATTCACTTGCAACAGGAAT+AGG 0.379182 8:+34545914 None:intergenic
TGGCAGACTGGGATAGTTCC+AGG 0.384913 8:+34545968 None:intergenic
CAGCCTTGTTACCAACAAAA+TGG 0.391110 8:+34545948 None:intergenic
ATTCAACCATTCCGAACAAT+AGG 0.392465 8:-34546424 MsG0880044028.01.T01:CDS
TTTAATAATGCAGACATTGA+AGG 0.399608 8:-34546517 MsG0880044028.01.T01:CDS
GATTCATACCTCTTGTATCA+TGG 0.417147 8:+34546023 None:intergenic
ATATCCAAATATCATCTAGA+TGG 0.433321 8:+34545874 None:intergenic
TTACTTCTTCCACTACATAT+TGG 0.433811 8:+34546194 None:intergenic
GTTCCATTGTTGGCCTCTGA+TGG 0.457551 8:-34546076 MsG0880044028.01.T01:CDS
TATAGTCTTGACGAGATTTG+TGG 0.471610 8:-34546343 MsG0880044028.01.T01:CDS
TCATCAGATTCACTTGCAAC+AGG 0.481559 8:+34545908 None:intergenic
AACTTGTCTTCTCCGATAGC+AGG 0.508034 8:+34546313 None:intergenic
TTGGAAAATGACCTATACCC+TGG 0.518567 8:-34546481 MsG0880044028.01.T01:CDS
TCGGCAACCATCCTATTGTT+CGG 0.521145 8:+34546413 None:intergenic
AACCATCCTATTGTTCGGAA+TGG 0.524994 8:+34546418 None:intergenic
AACATCCTCCGAATCAATGA+AGG 0.526354 8:-34546284 MsG0880044028.01.T01:CDS
GAAGTTCTCAAAGTCAAGCC+TGG 0.528095 8:-34545986 MsG0880044028.01.T01:CDS
GAATTTGCCATTTCAAAACT+CGG 0.533199 8:-34546262 MsG0880044028.01.T01:CDS
AATTTGCCATTTCAAAACTC+GGG 0.540236 8:-34546261 MsG0880044028.01.T01:CDS
TTGGTAACAAGGCTGTTGCT+TGG 0.540533 8:-34545940 MsG0880044028.01.T01:CDS
TGTGGAGGTCCACCTGCTAT+CGG 0.544677 8:-34546325 MsG0880044028.01.T01:CDS
TACCAACAAAATGGCAGACT+GGG 0.549171 8:+34545957 None:intergenic
TTACCAACAAAATGGCAGAC+TGG 0.565164 8:+34545956 None:intergenic
TTGTTGATGCATTCACTTGG+TGG 0.566717 8:+34546107 None:intergenic
GAATGCATCAACAACTTACA+TGG 0.567371 8:-34546098 MsG0880044028.01.T01:CDS
AAATTCCTTCATTGATTCGG+AGG 0.568883 8:+34546279 None:intergenic
GTAAAGAAAATTGCAGACAA+AGG 0.575220 8:-34546175 MsG0880044028.01.T01:CDS
TGGAACTAAAGTTAATGCAT+TGG 0.576995 8:-34546056 MsG0880044028.01.T01:CDS
ATTAACTTTAGTTCCATCAG+AGG 0.588740 8:+34546063 None:intergenic
GTTCCATCAGAGGCCAACAA+TGG 0.589021 8:+34546073 None:intergenic
TTCTCCACTACATTTCCAGA+CGG 0.593237 8:+34546373 None:intergenic
AACCATTCCGAACAATAGGA+TGG 0.593760 8:-34546420 MsG0880044028.01.T01:CDS
AAATCAAGACCAATATGTAG+TGG 0.595851 8:-34546203 MsG0880044028.01.T01:CDS
AGTCTTGACGAGATTTGTGG+AGG 0.619391 8:-34546340 MsG0880044028.01.T01:CDS
TTGTCTTCTCCGATAGCAGG+TGG 0.637131 8:+34546316 None:intergenic
TCAACCGTCTGGAAATGTAG+TGG 0.641252 8:-34546377 MsG0880044028.01.T01:CDS
TACCCTGGCAAGAAAATGAG+CGG 0.655096 8:-34546466 MsG0880044028.01.T01:CDS
AAGTTGTTGATGCATTCACT+TGG 0.662304 8:+34546104 None:intergenic
GCTTGTCACCATGATACAAG+AGG 0.667198 8:-34546031 MsG0880044028.01.T01:CDS
AATATCATCTAGATGGCACA+AGG 0.675245 8:+34545881 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACGAAAATTAAGTCATATTC+AGG - Chr8:34546174-34546193 MsG0880044028.01.T01:CDS 25.0%
! TTTAATAATGCAGACATTGA+AGG - Chr8:34545895-34545914 MsG0880044028.01.T01:CDS 25.0%
AAATCAAGACCAATATGTAG+TGG - Chr8:34546209-34546228 MsG0880044028.01.T01:CDS 30.0%
AGAACTTCATCAAGCAAATT+TGG + Chr8:34546414-34546433 None:intergenic 30.0%
GTAAAGAAAATTGCAGACAA+AGG - Chr8:34546237-34546256 MsG0880044028.01.T01:CDS 30.0%
TGGAACTAAAGTTAATGCAT+TGG - Chr8:34546356-34546375 MsG0880044028.01.T01:CDS 30.0%
TTACTTCTTCCACTACATAT+TGG + Chr8:34546221-34546240 None:intergenic 30.0%
! AATTTGCCATTTCAAAACTC+GGG - Chr8:34546151-34546170 MsG0880044028.01.T01:CDS 30.0%
! ATTAACTTTAGTTCCATCAG+AGG + Chr8:34546352-34546371 None:intergenic 30.0%
! GAATTTGCCATTTCAAAACT+CGG - Chr8:34546150-34546169 MsG0880044028.01.T01:CDS 30.0%
! GTTGAATATTGGAAAGCTTT+TGG + Chr8:34545975-34545994 None:intergenic 30.0%
! TGAAGGCAAATTACTCTTTT+TGG - Chr8:34545912-34545931 MsG0880044028.01.T01:CDS 30.0%
AACTTACATGGTTCCATTGT+TGG - Chr8:34546326-34546345 MsG0880044028.01.T01:CDS 35.0%
AAGTTGTTGATGCATTCACT+TGG + Chr8:34546311-34546330 None:intergenic 35.0%
ATTCAACCATTCCGAACAAT+AGG - Chr8:34545988-34546007 MsG0880044028.01.T01:CDS 35.0%
GAATGCATCAACAACTTACA+TGG - Chr8:34546314-34546333 MsG0880044028.01.T01:CDS 35.0%
GATTCATACCTCTTGTATCA+TGG + Chr8:34546392-34546411 None:intergenic 35.0%
TGTTCGGAATGGTTGAATAT+TGG + Chr8:34545986-34546005 None:intergenic 35.0%
! GGCAAATTCCTTCATTGATT+CGG + Chr8:34546139-34546158 None:intergenic 35.0%
! TATAGTCTTGACGAGATTTG+TGG - Chr8:34546069-34546088 MsG0880044028.01.T01:CDS 35.0%
!! AAATTCCTTCATTGATTCGG+AGG + Chr8:34546136-34546155 None:intergenic 35.0%
AACATCCTCCGAATCAATGA+AGG - Chr8:34546128-34546147 MsG0880044028.01.T01:CDS 40.0%
AACCATCCTATTGTTCGGAA+TGG + Chr8:34545997-34546016 None:intergenic 40.0%
AACCATTCCGAACAATAGGA+TGG - Chr8:34545992-34546011 MsG0880044028.01.T01:CDS 40.0%
GATTCACTTGCAACAGGAAT+AGG + Chr8:34546501-34546520 None:intergenic 40.0%
TACCAACAAAATGGCAGACT+GGG + Chr8:34546458-34546477 None:intergenic 40.0%
TCATCAGATTCACTTGCAAC+AGG + Chr8:34546507-34546526 None:intergenic 40.0%
TTACCAACAAAATGGCAGAC+TGG + Chr8:34546459-34546478 None:intergenic 40.0%
TTCTCCACTACATTTCCAGA+CGG + Chr8:34546042-34546061 None:intergenic 40.0%
TTGGAAAATGACCTATACCC+TGG - Chr8:34545931-34545950 MsG0880044028.01.T01:CDS 40.0%
TTGTTGATGCATTCACTTGG+TGG + Chr8:34546308-34546327 None:intergenic 40.0%
! TCATTTTCTTGCCAGGGTAT+AGG + Chr8:34545945-34545964 None:intergenic 40.0%
!! CAGCCTTGTTACCAACAAAA+TGG + Chr8:34546467-34546486 None:intergenic 40.0%
!!! CTGCCATTTTGTTGGTAACA+AGG - Chr8:34546461-34546480 MsG0880044028.01.T01:CDS 40.0%
!!! GGTTGAGTAGCTTTTTCGAT+CGG + Chr8:34546021-34546040 None:intergenic 40.0%
!!! TTTCGTCCCGAGTTTTGAAA+TGG + Chr8:34546160-34546179 None:intergenic 40.0%
AACTTGTCTTCTCCGATAGC+AGG + Chr8:34546102-34546121 None:intergenic 45.0%
AGTCTTGACGAGATTTGTGG+AGG - Chr8:34546072-34546091 MsG0880044028.01.T01:CDS 45.0%
GAAAAAGCTACTCAACCGTC+TGG - Chr8:34546024-34546043 MsG0880044028.01.T01:CDS 45.0%
GAAGTTCTCAAAGTCAAGCC+TGG - Chr8:34546426-34546445 MsG0880044028.01.T01:CDS 45.0%
GCTTGTCACCATGATACAAG+AGG - Chr8:34546381-34546400 MsG0880044028.01.T01:CDS 45.0%
TACCCTGGCAAGAAAATGAG+CGG - Chr8:34545946-34545965 MsG0880044028.01.T01:CDS 45.0%
TCAACCGTCTGGAAATGTAG+TGG - Chr8:34546035-34546054 MsG0880044028.01.T01:CDS 45.0%
TCGGCAACCATCCTATTGTT+CGG + Chr8:34546002-34546021 None:intergenic 45.0%
! TTGGTAACAAGGCTGTTGCT+TGG - Chr8:34546472-34546491 MsG0880044028.01.T01:CDS 45.0%
!!! ATCCCAGTCTGCCATTTTGT+TGG - Chr8:34546453-34546472 MsG0880044028.01.T01:CDS 45.0%
GTTCCATCAGAGGCCAACAA+TGG + Chr8:34546342-34546361 None:intergenic 50.0%
TTGTCTTCTCCGATAGCAGG+TGG + Chr8:34546099-34546118 None:intergenic 50.0%
! AGACCGCTCATTTTCTTGCC+AGG + Chr8:34545952-34545971 None:intergenic 50.0%
! GACCGCTCATTTTCTTGCCA+GGG + Chr8:34545951-34545970 None:intergenic 50.0%
! GTTCCATTGTTGGCCTCTGA+TGG - Chr8:34546336-34546355 MsG0880044028.01.T01:CDS 50.0%
TGGCAGACTGGGATAGTTCC+AGG + Chr8:34546447-34546466 None:intergenic 55.0%
TGTGGAGGTCCACCTGCTAT+CGG - Chr8:34546087-34546106 MsG0880044028.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 34545889 34546545 34545889 ID=MsG0880044028.01;Name=MsG0880044028.01
Chr8 mRNA 34545889 34546545 34545889 ID=MsG0880044028.01.T01;Parent=MsG0880044028.01;Name=MsG0880044028.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|218
Chr8 exon 34545889 34546545 34545889 ID=MsG0880044028.01.T01:exon:3652;Parent=MsG0880044028.01.T01
Chr8 CDS 34545889 34546545 34545889 ID=MsG0880044028.01.T01:cds;Parent=MsG0880044028.01.T01
Gene Sequence

>MsG0880044028.01.T01

ATGATTTTTAATAATGCAGACATTGAAGGCAAATTACTCTTTTTGGAAAATGACCTATACCCTGGCAAGAAAATGAGCGGTCTCCAAAAGCTTTCCAATATTCAACCATTCCGAACAATAGGATGGTTGCCGATCGAAAAAGCTACTCAACCGTCTGGAAATGTAGTGGAGAAAGAGAGTTATAGTCTTGACGAGATTTGTGGAGGTCCACCTGCTATCGGAGAAGACAAGTTTTGCGCAACATCCTCCGAATCAATGAAGGAATTTGCCATTTCAAAACTCGGGACGAAAATTAAGTCATATTCAGGTTACTTTGCTAAAAATCAAGACCAATATGTAGTGGAAGAAGTAAAGAAAATTGCAGACAAAGGAGTAATGTGTCATCGAATGAATTTCGAGAAAGTTGTATTTTATTGCCACCAAGTGAATGCATCAACAACTTACATGGTTCCATTGTTGGCCTCTGATGGAACTAAAGTTAATGCATTGGCTGCTTGTCACCATGATACAAGAGGTATGAATCCAAATTTGCTTGATGAAGTTCTCAAAGTCAAGCCTGGAACTATCCCAGTCTGCCATTTTGTTGGTAACAAGGCTGTTGCTTGGTTACCTATTCCTGTTGCAAGTGAATCTGATGATCGTCCTTGTGCCATCTAG

Protein sequence

>MsG0880044028.01.T01

MIFNNADIEGKLLFLENDLYPGKKMSGLQKLSNIQPFRTIGWLPIEKATQPSGNVVEKESYSLDEICGGPPAIGEDKFCATSSESMKEFAISKLGTKIKSYSGYFAKNQDQYVVEEVKKIADKGVMCHRMNFEKVVFYCHQVNASTTYMVPLLASDGTKVNALAACHHDTRGMNPNLLDEVLKVKPGTIPVCHFVGNKAVAWLPIPVASESDDRPCAI*