AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021403.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021403.01.T01 MTR_4g069090 45.418 251 82 8 18 252 109 320 6.08e-44 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021403.01.T01 AT3G18860 37.849 251 101 8 18 252 112 323 8.01e-35 132
MsG0480021403.01.T01 AT3G18860 37.849 251 101 8 18 252 112 323 8.57e-35 132

Find 52 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTGGGATACAAAATTTA+TGG 0.163017 4:+57456104 MsG0480021403.01.T01:CDS
ACTACAGTTCTCTCAGATTA+TGG 0.178467 4:+57455378 MsG0480021403.01.T01:intron
TGTAAAATCAGCGATTTATT+TGG 0.197248 4:+57454142 MsG0480021403.01.T01:intron
TTTGCTGCGATGTCTGATTT+TGG 0.217376 4:+57455235 MsG0480021403.01.T01:CDS
CTCAGATTATGGGCATTAAA+TGG 0.298081 4:+57455389 MsG0480021403.01.T01:CDS
GTCAATTCGATCGGTGATAA+TGG 0.299351 4:-57455897 None:intergenic
CTTGTTTCATCTTCTATTGA+TGG 0.317174 4:+57454046 MsG0480021403.01.T01:CDS
GAAGATCGTTTGGCAAAATA+TGG 0.327645 4:+57455497 MsG0480021403.01.T01:CDS
CCGATGGAGTTGTACGCATT+TGG 0.342372 4:+57456153 MsG0480021403.01.T01:CDS
TGTTGTGCTGGATCTTGGTT+TGG 0.366368 4:+57454227 MsG0480021403.01.T01:CDS
CTACAGTTCTCTCAGATTAT+GGG 0.367037 4:+57455379 MsG0480021403.01.T01:intron
GATCATGAATCGGAGGTTAC+TGG 0.380364 4:+57454001 MsG0480021403.01.T01:CDS
CAGTGGTAGCGAAGATCGTT+TGG 0.399445 4:+57455487 MsG0480021403.01.T01:CDS
GTTACTGGTATTGTGGTTGA+TGG 0.414174 4:+57454016 MsG0480021403.01.T01:CDS
GATACAAAATTTATGGAAAA+CGG 0.420447 4:+57456111 MsG0480021403.01.T01:CDS
GAGGATGTTGTGCTGGATCT+TGG 0.426325 4:+57454222 MsG0480021403.01.T01:CDS
ATACAAAATTTATGGAAAAC+GGG 0.432431 4:+57456112 MsG0480021403.01.T01:CDS
GTTGTGCTGGATCTTGGTTT+GGG 0.433871 4:+57454228 MsG0480021403.01.T01:CDS
CTGAACAAACAGTTGCAGTA+TGG 0.447329 4:-57455436 None:intergenic
TCAGTTGATTCACATGCATC+AGG 0.449667 4:+57455455 MsG0480021403.01.T01:CDS
CCTACTCTGACCGAATGATT+AGG 0.450689 4:+57453804 None:intergenic
ACCCATCATGGGATGCAGAT+AGG 0.452110 4:-57455261 None:intergenic
TTGTTTCATCTTCTATTGAT+GGG 0.457455 4:+57454047 MsG0480021403.01.T01:CDS
GACGTGCATGTGAAGCATCC+AGG 0.464595 4:+57455917 MsG0480021403.01.T01:CDS
TCCTATCTGCATCCCATGAT+GGG 0.476764 4:+57455260 MsG0480021403.01.T01:CDS
ACACTTATTATTCTCTGTGT+TGG 0.481013 4:-57454190 None:intergenic
AGAAATGAACCCACCCATCA+TGG 0.491698 4:-57455273 None:intergenic
CATCAGATAATGAACATGTC+TGG 0.498451 4:+57455413 MsG0480021403.01.T01:CDS
TTATTTGGTGATAAAGTGTT+GGG 0.500390 4:+57454157 MsG0480021403.01.T01:CDS
TTTATTTGGTGATAAAGTGT+TGG 0.509140 4:+57454156 MsG0480021403.01.T01:CDS
CTGAGATGAGGATGTTGTGC+TGG 0.518958 4:+57454215 MsG0480021403.01.T01:CDS
ATCCTATCTGCATCCCATGA+TGG 0.540343 4:+57455259 MsG0480021403.01.T01:CDS
TCTGACCGAATGATTAGGCT+TGG 0.541651 4:+57453809 None:intergenic
GAATGTACAGATACTGTTTG+TGG 0.547131 4:+57455211 MsG0480021403.01.T01:intron
GTTCAGAGCATAGAGCATCC+TGG 0.560137 4:+57456078 MsG0480021403.01.T01:CDS
ACGTGCATGTGAAGCATCCA+GGG 0.570780 4:+57455918 MsG0480021403.01.T01:CDS
GAAGACACTAGATCATGAAT+CGG 0.571511 4:+57453991 MsG0480021403.01.T01:CDS
GAAATGAACCCACCCATCAT+GGG 0.575781 4:-57455272 None:intergenic
TTTGTATCCCAAACAAAACC+AGG 0.581250 4:-57456096 None:intergenic
TAATAAGTGTAGCTGAGATG+AGG 0.594885 4:+57454203 MsG0480021403.01.T01:CDS
TGGTTTGGGCAAGTAGCAAG+AGG 0.606236 4:+57454242 MsG0480021403.01.T01:CDS
TGTATAAATCAAGTTCTCGC+TGG 0.611367 4:-57456202 None:intergenic
TGATTAGGCTTGGACAGTCA+CGG 0.613504 4:+57453819 MsG0480021403.01.T01:CDS
CACATGCACGTCAATTCGAT+CGG 0.614319 4:-57455906 None:intergenic
ATCGGAGGTTACTGGTATTG+TGG 0.622916 4:+57454009 MsG0480021403.01.T01:CDS
CCAAATGCGTACAACTCCAT+CGG 0.622939 4:-57456153 None:intergenic
TCGTTTGGCAAAATATGGAA+AGG 0.628837 4:+57455502 MsG0480021403.01.T01:CDS
ATTGTGACAGCATGTTCCGA+TGG 0.634535 4:+57456137 MsG0480021403.01.T01:CDS
GACACTAGATCATGAATCGG+AGG 0.635591 4:+57453994 MsG0480021403.01.T01:CDS
GCATCAGGACTTATTGTCAG+TGG 0.647307 4:+57455470 MsG0480021403.01.T01:CDS
TTTGGTGATAAAGTGTTGGG+TGG 0.656701 4:+57454160 MsG0480021403.01.T01:CDS
TCGGTGATAATGGAAGAGAC+TGG 0.664017 4:-57455887 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAAATTTATTAAATCTAG+AGG - Chr4:57455308-57455327 None:intergenic 15.0%
!!! ACTTTGTTTTCTTTATATTT+TGG + Chr4:57455014-57455033 MsG0480021403.01.T01:intron 15.0%
!!! CTTATTTCTATTTTATATTG+AGG + Chr4:57455783-57455802 MsG0480021403.01.T01:intron 15.0%
!!! TATTTTAGAAGAAAAGAAAA+TGG + Chr4:57455830-57455849 MsG0480021403.01.T01:CDS 15.0%
!!! TTTGTCTTTATGAATTTTAT+GGG + Chr4:57455647-57455666 MsG0480021403.01.T01:intron 15.0%
!! AACTATAGAGATTAATGAAT+TGG + Chr4:57454341-57454360 MsG0480021403.01.T01:intron 20.0%
!! ATACAAAATTTATGGAAAAC+GGG + Chr4:57456112-57456131 MsG0480021403.01.T01:CDS 20.0%
!! GATACAAAATTTATGGAAAA+CGG + Chr4:57456111-57456130 MsG0480021403.01.T01:CDS 20.0%
!!! AATAAACAAACTAGCTTATA+TGG - Chr4:57455814-57455833 None:intergenic 20.0%
!!! ATTTTGTCATCGATTTATAA+TGG + Chr4:57454964-57454983 MsG0480021403.01.T01:intron 20.0%
!!! GTGTTATTAATTCATATAAG+AGG - Chr4:57455169-57455188 None:intergenic 20.0%
!!! GTTTGTCTTTATGAATTTTA+TGG + Chr4:57455646-57455665 MsG0480021403.01.T01:intron 20.0%
!!! TTGTCTTTATGAATTTTATG+GGG + Chr4:57455648-57455667 MsG0480021403.01.T01:intron 20.0%
! AATCTGCTTGATTAACATTT+CGG + Chr4:57455731-57455750 MsG0480021403.01.T01:intron 25.0%
! TAAAATGTGTGTCTAAAGAT+GGG - Chr4:57454937-57454956 None:intergenic 25.0%
! TGTAAAATCAGCGATTTATT+TGG + Chr4:57454142-57454161 MsG0480021403.01.T01:intron 25.0%
! TTAAAATGTGTGTCTAAAGA+TGG - Chr4:57454938-57454957 None:intergenic 25.0%
! TTAACTTATAGCTCTTTGTT+TGG + Chr4:57454987-57455006 MsG0480021403.01.T01:intron 25.0%
! TTGTTTCATCTTCTATTGAT+GGG + Chr4:57454047-57454066 MsG0480021403.01.T01:CDS 25.0%
!! AACTTATATTCTCCCATTTT+AGG + Chr4:57454817-57454836 MsG0480021403.01.T01:intron 25.0%
!! AGTTGTTTACATACTTTTCA+TGG + Chr4:57454894-57454913 MsG0480021403.01.T01:intron 25.0%
!! TGTTTGGGATACAAAATTTA+TGG + Chr4:57456104-57456123 MsG0480021403.01.T01:CDS 25.0%
!! TTATTTGGTGATAAAGTGTT+GGG + Chr4:57454157-57454176 MsG0480021403.01.T01:CDS 25.0%
!! TTTATTTGGTGATAAAGTGT+TGG + Chr4:57454156-57454175 MsG0480021403.01.T01:CDS 25.0%
!!! TACAGGTATTGATACATTTT+CGG + Chr4:57454695-57454714 MsG0480021403.01.T01:intron 25.0%
AACAAGTCAATGCACAAAAA+AGG + Chr4:57456242-57456261 MsG0480021403.01.T01:CDS 30.0%
AATTACTGCACAAGAAATTC+AGG - Chr4:57454573-57454592 None:intergenic 30.0%
ACACTTATTATTCTCTGTGT+TGG - Chr4:57454193-57454212 None:intergenic 30.0%
ATAGCAATAATGTTTCCTTG+TGG + Chr4:57455070-57455089 MsG0480021403.01.T01:intron 30.0%
CAATGCACAAAAAAGGAAAT+TGG + Chr4:57456249-57456268 MsG0480021403.01.T01:CDS 30.0%
CTTGTTTCATCTTCTATTGA+TGG + Chr4:57454046-57454065 MsG0480021403.01.T01:CDS 30.0%
GAAATAAGTTGCAATAACAG+CGG - Chr4:57455771-57455790 None:intergenic 30.0%
GGCGAAAAAACTAAAACTAT+CGG - Chr4:57455988-57456007 None:intergenic 30.0%
GTTGTGTAGATTCTTTAGTA+AGG + Chr4:57454264-57454283 MsG0480021403.01.T01:intron 30.0%
TATCGGCCATGTAAATAAAA+CGG - Chr4:57455971-57455990 None:intergenic 30.0%
TCAGTCCAATACATTTCAAA+TGG - Chr4:57454784-57454803 None:intergenic 30.0%
TTTGACTATCATTCATCTGA+TGG - Chr4:57454294-57454313 None:intergenic 30.0%
! AACTTTTCATTACCACTTGT+TGG + Chr4:57454722-57454741 MsG0480021403.01.T01:intron 30.0%
! ACTTTTCATTACCACTTGTT+GGG + Chr4:57454723-57454742 MsG0480021403.01.T01:intron 30.0%
! AGCTTTATAACTGCTTGAAT+TGG - Chr4:57454657-57454676 None:intergenic 30.0%
! CAAGCAGATTCTTACAATTT+TGG - Chr4:57455721-57455740 None:intergenic 30.0%
! GTAAATCAGAATCATTTTGC+TGG + Chr4:57456179-57456198 MsG0480021403.01.T01:CDS 30.0%
! TATATTCTTTTATGCTGCCA+GGG + Chr4:57455113-57455132 MsG0480021403.01.T01:intron 30.0%
! TATCAAAAGATGTTTTCGAC+AGG - Chr4:57453851-57453870 None:intergenic 30.0%
! TCACACCATTTGAAATGTAT+TGG + Chr4:57454776-57454795 MsG0480021403.01.T01:intron 30.0%
! TCTAGTTTAAAGATCTGGAA+GGG + Chr4:57454868-57454887 MsG0480021403.01.T01:intron 30.0%
! TTATATTCTTTTATGCTGCC+AGG + Chr4:57455112-57455131 MsG0480021403.01.T01:intron 30.0%
! TTTTATGGGGATTTTTGTTG+TGG + Chr4:57455661-57455680 MsG0480021403.01.T01:intron 30.0%
!! TGTTCTTTTTAGTGTCCTTA+GGG + Chr4:57455605-57455624 MsG0480021403.01.T01:intron 30.0%
!! TTGTTCTTTTTAGTGTCCTT+AGG + Chr4:57455604-57455623 MsG0480021403.01.T01:intron 30.0%
!! TTTATGGGGATTTTTGTTGT+GGG + Chr4:57455662-57455681 MsG0480021403.01.T01:intron 30.0%
!!! TTTAATTTTTAGCCACTCGA+AGG + Chr4:57455942-57455961 MsG0480021403.01.T01:intron 30.0%
AATCCATGATATACATCGGA+CGG - Chr4:57453885-57453904 None:intergenic 35.0%
AATGGGACTTGAACCTAAAA+TGG - Chr4:57454833-57454852 None:intergenic 35.0%
ACTACAGTTCTCTCAGATTA+TGG + Chr4:57455378-57455397 MsG0480021403.01.T01:intron 35.0%
AGGGAGCTTAAGATTAATCT+TGG + Chr4:57455624-57455643 MsG0480021403.01.T01:intron 35.0%
AGTATCTGTACATTCGTCAA+AGG - Chr4:57455206-57455225 None:intergenic 35.0%
AGTCAAATTACCAGAGACAT+AGG + Chr4:57454307-57454326 MsG0480021403.01.T01:intron 35.0%
ATGGGACTTGAACCTAAAAT+GGG - Chr4:57454832-57454851 None:intergenic 35.0%
ATTTGAAGAGGTGGAGTAAT+TGG + Chr4:57454590-57454609 MsG0480021403.01.T01:intron 35.0%
CACTCAGAATTATGTCTCTT+AGG + Chr4:57454749-57454768 MsG0480021403.01.T01:intron 35.0%
CATCAGATAATGAACATGTC+TGG + Chr4:57455413-57455432 MsG0480021403.01.T01:CDS 35.0%
CCTTTATGTCCTAAAATCGA+AGG - Chr4:57455046-57455065 None:intergenic 35.0%
CTACAGTTCTCTCAGATTAT+GGG + Chr4:57455379-57455398 MsG0480021403.01.T01:intron 35.0%
CTCAATACTGTTGAGTTTCA+TGG + Chr4:57455578-57455597 MsG0480021403.01.T01:intron 35.0%
CTCAGATTATGGGCATTAAA+TGG + Chr4:57455389-57455408 MsG0480021403.01.T01:CDS 35.0%
GAAGACACTAGATCATGAAT+CGG + Chr4:57453991-57454010 MsG0480021403.01.T01:CDS 35.0%
GAAGATCGTTTGGCAAAATA+TGG + Chr4:57455497-57455516 MsG0480021403.01.T01:CDS 35.0%
GAATGTACAGATACTGTTTG+TGG + Chr4:57455211-57455230 MsG0480021403.01.T01:intron 35.0%
GATTAATCTTAAGCTCCCTA+AGG - Chr4:57455623-57455642 None:intergenic 35.0%
GTGGAATCCATGATATACAT+CGG - Chr4:57453889-57453908 None:intergenic 35.0%
GTGGCTAAAAATTAAAACCC+TGG - Chr4:57455938-57455957 None:intergenic 35.0%
TAATAAGTGTAGCTGAGATG+AGG + Chr4:57454203-57454222 MsG0480021403.01.T01:CDS 35.0%
TCGTTTGGCAAAATATGGAA+AGG + Chr4:57455502-57455521 MsG0480021403.01.T01:CDS 35.0%
TCTTGTGCAGTAATTTGAAG+AGG + Chr4:57454578-57454597 MsG0480021403.01.T01:intron 35.0%
TGTATAAATCAAGTTCTCGC+TGG - Chr4:57456205-57456224 None:intergenic 35.0%
TGTCATTCCATTCCTTATTC+CGG + Chr4:57455695-57455714 MsG0480021403.01.T01:intron 35.0%
TTAAAGTCTTCCTATGTCTC+TGG - Chr4:57454320-57454339 None:intergenic 35.0%
TTGGAATTGTTCGAATTGCA+CGG + Chr4:57454609-57454628 MsG0480021403.01.T01:intron 35.0%
TTTGTATCCCAAACAAAACC+AGG - Chr4:57456099-57456118 None:intergenic 35.0%
! CTCTAGTTTAAAGATCTGGA+AGG + Chr4:57454867-57454886 MsG0480021403.01.T01:intron 35.0%
! TCATCTTCTATTGATGGGTA+GGG + Chr4:57454052-57454071 MsG0480021403.01.T01:intron 35.0%
! TTAGGTTCAAGTCCCATTTT+AGG + Chr4:57454835-57454854 MsG0480021403.01.T01:intron 35.0%
! TTCATCTTCTATTGATGGGT+AGG + Chr4:57454051-57454070 MsG0480021403.01.T01:intron 35.0%
!! CAGATTCTTACAATTTTGGC+CGG - Chr4:57455717-57455736 None:intergenic 35.0%
!! TTACAATTTTGGCCGGAATA+AGG - Chr4:57455710-57455729 None:intergenic 35.0%
!! TTATGGGGATTTTTGTTGTG+GGG + Chr4:57455663-57455682 MsG0480021403.01.T01:intron 35.0%
!!! AAGTCCCATTTTAGGTTCAA+GGG + Chr4:57454843-57454862 MsG0480021403.01.T01:intron 35.0%
!!! AGGCATCCGTTTTATTTACA+TGG + Chr4:57455962-57455981 MsG0480021403.01.T01:intron 35.0%
!!! ATGCTGTGGTTTTTTTTACG+TGG + Chr4:57453954-57453973 MsG0480021403.01.T01:intron 35.0%
!!! CCTTCGATTTTAGGACATAA+AGG + Chr4:57455043-57455062 MsG0480021403.01.T01:intron 35.0%
AGAGTCCCTTGAACCTAAAA+TGG - Chr4:57454851-57454870 None:intergenic 40.0%
ATATATTCCTGATGCAGAGC+AGG - Chr4:57455333-57455352 None:intergenic 40.0%
ATCCATGATATACATCGGAC+GGG - Chr4:57453884-57453903 None:intergenic 40.0%
CTGAACAAACAGTTGCAGTA+TGG - Chr4:57455439-57455458 None:intergenic 40.0%
GAGTCCCTTGAACCTAAAAT+GGG - Chr4:57454850-57454869 None:intergenic 40.0%
GTAACAGAAACAAGTCAAGC+AGG - Chr4:57455360-57455379 None:intergenic 40.0%
GTCAATTCGATCGGTGATAA+TGG - Chr4:57455900-57455919 None:intergenic 40.0%
GTTACTGGTATTGTGGTTGA+TGG + Chr4:57454016-57454035 MsG0480021403.01.T01:CDS 40.0%
GTTGCAATAACAGCGGATAT+TGG - Chr4:57455764-57455783 None:intergenic 40.0%
GTTTCCTTGTGGACATGATT+TGG + Chr4:57455081-57455100 MsG0480021403.01.T01:intron 40.0%
TGTGCAGTAATTTGAAGAGG+TGG + Chr4:57454581-57454600 MsG0480021403.01.T01:intron 40.0%
! CATACTTTTCATGGACATGC+AGG + Chr4:57454903-57454922 MsG0480021403.01.T01:intron 40.0%
! GGGACTCTAGTTTAAAGATC+TGG + Chr4:57454863-57454882 MsG0480021403.01.T01:intron 40.0%
! TCAGTTGATTCACATGCATC+AGG + Chr4:57455455-57455474 MsG0480021403.01.T01:CDS 40.0%
! TTTGCTGCGATGTCTGATTT+TGG + Chr4:57455235-57455254 MsG0480021403.01.T01:CDS 40.0%
!! ATTTTGGCCGGAATAAGGAA+TGG - Chr4:57455705-57455724 None:intergenic 40.0%
!! TATGGGGATTTTTGTTGTGG+GGG + Chr4:57455664-57455683 MsG0480021403.01.T01:intron 40.0%
!! TTTGGTGATAAAGTGTTGGG+TGG + Chr4:57454160-57454179 MsG0480021403.01.T01:CDS 40.0%
!!! AGAGCATCCTGGTTTTGTTT+GGG + Chr4:57456089-57456108 MsG0480021403.01.T01:CDS 40.0%
!!! CAAGTCCCATTTTAGGTTCA+AGG + Chr4:57454842-57454861 MsG0480021403.01.T01:intron 40.0%
!!! GTGGTTTTTTTTACGTGGAG+TGG + Chr4:57453959-57453978 MsG0480021403.01.T01:intron 40.0%
!!! TAGAGCATCCTGGTTTTGTT+TGG + Chr4:57456088-57456107 MsG0480021403.01.T01:CDS 40.0%
!!! TAGTTTTTTCGCCACATCAC+AGG + Chr4:57455995-57456014 MsG0480021403.01.T01:intron 40.0%
ACTGCCAAATCATGTCCACA+AGG - Chr4:57455088-57455107 None:intergenic 45.0%
AGAAATGAACCCACCCATCA+TGG - Chr4:57455276-57455295 None:intergenic 45.0%
AGCAACAAGTTCCTGTGATG+TGG - Chr4:57456009-57456028 None:intergenic 45.0%
ATAAAACGGATGCCTTCGAG+TGG - Chr4:57455957-57455976 None:intergenic 45.0%
ATCCTATCTGCATCCCATGA+TGG + Chr4:57455259-57455278 MsG0480021403.01.T01:CDS 45.0%
ATCGGAGGTTACTGGTATTG+TGG + Chr4:57454009-57454028 MsG0480021403.01.T01:CDS 45.0%
ATTGTGACAGCATGTTCCGA+TGG + Chr4:57456137-57456156 MsG0480021403.01.T01:CDS 45.0%
CACATGCACGTCAATTCGAT+CGG - Chr4:57455909-57455928 None:intergenic 45.0%
CAGCAAAAATATGCAGTCCC+TGG - Chr4:57455133-57455152 None:intergenic 45.0%
CCAAATGCGTACAACTCCAT+CGG - Chr4:57456156-57456175 None:intergenic 45.0%
CTGGTGTGTGAATAATGCTG+TGG + Chr4:57453940-57453959 MsG0480021403.01.T01:intron 45.0%
GAAATGAACCCACCCATCAT+GGG - Chr4:57455275-57455294 None:intergenic 45.0%
GACACTAGATCATGAATCGG+AGG + Chr4:57453994-57454013 MsG0480021403.01.T01:CDS 45.0%
GATCATGAATCGGAGGTTAC+TGG + Chr4:57454001-57454020 MsG0480021403.01.T01:CDS 45.0%
GCAGTTATAAAGCTACCCAC+AGG + Chr4:57454663-57454682 MsG0480021403.01.T01:intron 45.0%
GCATCAGGACTTATTGTCAG+TGG + Chr4:57455470-57455489 MsG0480021403.01.T01:CDS 45.0%
GTTGTGCTGGATCTTGGTTT+GGG + Chr4:57454228-57454247 MsG0480021403.01.T01:CDS 45.0%
TCCTATCTGCATCCCATGAT+GGG + Chr4:57455260-57455279 MsG0480021403.01.T01:CDS 45.0%
TCTGAGTGTAACCCAACAAG+TGG - Chr4:57454737-57454756 None:intergenic 45.0%
TGATTAGGCTTGGACAGTCA+CGG + Chr4:57453819-57453838 MsG0480021403.01.T01:CDS 45.0%
TGTTGTGCTGGATCTTGGTT+TGG + Chr4:57454227-57454246 MsG0480021403.01.T01:CDS 45.0%
TTCGAATTGCACGGAGACAT+GGG + Chr4:57454618-57454637 MsG0480021403.01.T01:intron 45.0%
! AACCCTACTTTGAAAAGCGG+TGG + Chr4:57453911-57453930 MsG0480021403.01.T01:intron 45.0%
! CCGAACCCTACTTTGAAAAG+CGG + Chr4:57453908-57453927 MsG0480021403.01.T01:intron 45.0%
! CTTTTCAAAGTAGGGTTCGG+TGG - Chr4:57453908-57453927 None:intergenic 45.0%
! TGGTCTGTGCCTTCGATTTT+AGG + Chr4:57455034-57455053 MsG0480021403.01.T01:intron 45.0%
!! CACCACCGCTTTTCAAAGTA+GGG - Chr4:57453916-57453935 None:intergenic 45.0%
!! CATCACAGGAACTTGTTGCT+TGG + Chr4:57456009-57456028 MsG0480021403.01.T01:intron 45.0%
!! CCGCTTTTCAAAGTAGGGTT+CGG - Chr4:57453911-57453930 None:intergenic 45.0%
!! TCGGTGATAATGGAAGAGAC+TGG - Chr4:57455890-57455909 None:intergenic 45.0%
ACCCATCATGGGATGCAGAT+AGG - Chr4:57455264-57455283 None:intergenic 50.0%
ACCTGTAACAAGCTCTCCTG+TGG - Chr4:57454682-57454701 None:intergenic 50.0%
AGCCCGTCCGATGTATATCA+TGG + Chr4:57453879-57453898 MsG0480021403.01.T01:intron 50.0%
CAAGCTTTGCCCACGAATCA+GGG - Chr4:57454417-57454436 None:intergenic 50.0%
CCGATGGAGTTGTACGCATT+TGG + Chr4:57456153-57456172 MsG0480021403.01.T01:CDS 50.0%
CCTGTAACAAGCTCTCCTGT+GGG - Chr4:57454681-57454700 None:intergenic 50.0%
CTGAGATGAGGATGTTGTGC+TGG + Chr4:57454215-57454234 MsG0480021403.01.T01:CDS 50.0%
GAGGATGTTGTGCTGGATCT+TGG + Chr4:57454222-57454241 MsG0480021403.01.T01:CDS 50.0%
GGTCGGCTACAGGGTATAAA+CGG - Chr4:57454396-57454415 None:intergenic 50.0%
GTTCGAATTGCACGGAGACA+TGG + Chr4:57454617-57454636 MsG0480021403.01.T01:intron 50.0%
TTGAATGCCTGCTCTGCATC+AGG + Chr4:57455323-57455342 MsG0480021403.01.T01:intron 50.0%
! ACGTGCATGTGAAGCATCCA+GGG + Chr4:57455918-57455937 MsG0480021403.01.T01:CDS 50.0%
! ATATACATCGGACGGGCTTG+CGG - Chr4:57453877-57453896 None:intergenic 50.0%
! CAGTGGTAGCGAAGATCGTT+TGG + Chr4:57455487-57455506 MsG0480021403.01.T01:CDS 50.0%
! CCTACTTTGAAAAGCGGTGG+TGG + Chr4:57453914-57453933 MsG0480021403.01.T01:intron 50.0%
! GTTCAGAGCATAGAGCATCC+TGG + Chr4:57456078-57456097 MsG0480021403.01.T01:CDS 50.0%
! TATCTGCATCCCATGATGGG+TGG + Chr4:57455263-57455282 MsG0480021403.01.T01:intron 50.0%
! TGAAAAGCGGTGGTGGTTTC+TGG + Chr4:57453921-57453940 MsG0480021403.01.T01:intron 50.0%
! TGGTTTGGGCAAGTAGCAAG+AGG + Chr4:57454242-57454261 MsG0480021403.01.T01:CDS 50.0%
!! ATCTGCATCCCATGATGGGT+GGG + Chr4:57455264-57455283 MsG0480021403.01.T01:intron 50.0%
!! ATTTTTGTTGTGGGGGTGGG+GGG + Chr4:57455671-57455690 MsG0480021403.01.T01:intron 50.0%
!! CCACCACCGCTTTTCAAAGT+AGG - Chr4:57453917-57453936 None:intergenic 50.0%
!! GATTTTTGTTGTGGGGGTGG+GGG + Chr4:57455670-57455689 MsG0480021403.01.T01:intron 50.0%
!! GGATTTTTGTTGTGGGGGTG+GGG + Chr4:57455669-57455688 MsG0480021403.01.T01:intron 50.0%
!! GGGATTTTTGTTGTGGGGGT+GGG + Chr4:57455668-57455687 MsG0480021403.01.T01:intron 50.0%
ACGAATCAGGGTCGGCTACA+GGG - Chr4:57454405-57454424 None:intergenic 55.0%
CCAAGCTTTGCCCACGAATC+AGG - Chr4:57454418-57454437 None:intergenic 55.0%
CTTTGCCCACGAATCAGGGT+CGG - Chr4:57454413-57454432 None:intergenic 55.0%
GAATTGCACGGAGACATGGG+CGG + Chr4:57454621-57454640 MsG0480021403.01.T01:intron 55.0%
! CCTGATTCGTGGGCAAAGCT+TGG + Chr4:57454415-57454434 MsG0480021403.01.T01:intron 55.0%
! GACGTGCATGTGAAGCATCC+AGG + Chr4:57455917-57455936 MsG0480021403.01.T01:CDS 55.0%
! TATACATCGGACGGGCTTGC+GGG - Chr4:57453876-57453895 None:intergenic 55.0%
! TGTAGCCGACCCTGATTCGT+GGG + Chr4:57454405-57454424 MsG0480021403.01.T01:intron 55.0%
!! CCCACAGGAGAGCTTGTTAC+AGG + Chr4:57454678-57454697 MsG0480021403.01.T01:intron 55.0%
!! GGGGATTTTTGTTGTGGGGG+TGG + Chr4:57455667-57455686 MsG0480021403.01.T01:intron 55.0%
CACGAATCAGGGTCGGCTAC+AGG - Chr4:57454406-57454425 None:intergenic 60.0%
! CTGTAGCCGACCCTGATTCG+TGG + Chr4:57454404-57454423 MsG0480021403.01.T01:intron 60.0%
GAGACATGGGCGGCTCACGA+GGG + Chr4:57454631-57454650 MsG0480021403.01.T01:intron 65.0%
GGAGACATGGGCGGCTCACG+AGG + Chr4:57454630-57454649 MsG0480021403.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 57453818 57456286 57453818 ID=MsG0480021403.01;Name=MsG0480021403.01
Chr4 mRNA 57453818 57456286 57453818 ID=MsG0480021403.01.T01;Parent=MsG0480021403.01;Name=MsG0480021403.01.T01;_AED=0.49;_eAED=0.66;_QI=0|0.16|0.28|1|1|1|7|0|259
Chr4 exon 57453818 57453850 57453818 ID=MsG0480021403.01.T01:exon:17148;Parent=MsG0480021403.01.T01
Chr4 exon 57453986 57454068 57453986 ID=MsG0480021403.01.T01:exon:17149;Parent=MsG0480021403.01.T01
Chr4 exon 57454153 57454280 57454153 ID=MsG0480021403.01.T01:exon:17150;Parent=MsG0480021403.01.T01
Chr4 exon 57455221 57455281 57455221 ID=MsG0480021403.01.T01:exon:17151;Parent=MsG0480021403.01.T01
Chr4 exon 57455385 57455523 57455385 ID=MsG0480021403.01.T01:exon:17152;Parent=MsG0480021403.01.T01
Chr4 exon 57455824 57455939 57455824 ID=MsG0480021403.01.T01:exon:17153;Parent=MsG0480021403.01.T01
Chr4 exon 57456067 57456286 57456067 ID=MsG0480021403.01.T01:exon:17154;Parent=MsG0480021403.01.T01
Chr4 CDS 57453818 57453850 57453818 ID=MsG0480021403.01.T01:cds;Parent=MsG0480021403.01.T01
Chr4 CDS 57453986 57454068 57453986 ID=MsG0480021403.01.T01:cds;Parent=MsG0480021403.01.T01
Chr4 CDS 57454153 57454280 57454153 ID=MsG0480021403.01.T01:cds;Parent=MsG0480021403.01.T01
Chr4 CDS 57455221 57455281 57455221 ID=MsG0480021403.01.T01:cds;Parent=MsG0480021403.01.T01
Chr4 CDS 57455385 57455523 57455385 ID=MsG0480021403.01.T01:cds;Parent=MsG0480021403.01.T01
Chr4 CDS 57455824 57455939 57455824 ID=MsG0480021403.01.T01:cds;Parent=MsG0480021403.01.T01
Chr4 CDS 57456067 57456286 57456067 ID=MsG0480021403.01.T01:cds;Parent=MsG0480021403.01.T01
Gene Sequence

>MsG0480021403.01.T01

ATGATTAGGCTTGGACAGTCACGGCGAAGACCTATTGTGAAGACACTAGATCATGAATCGGAGGTTACTGGTATTGTGGTTGATGGAGATGATCTTGTTTCATCTTCTATTGATGGCGATTTATTTGGTGATAAAGTGTTGGGTGGTAAAGAACCAACACAGAGAATAATAAGTGTAGCTGAGATGAGGATGTTGTGCTGGATCTTGGTTTGGGCAAGTAGCAAGAGGTTGTGTAGATTCTTTAATACTGTTTGTGGCTTTGCTGCGATGTCTGATTTTGGAATCCTATCTGCATCCCATGATGGTTCTCTCAGATTATGGGCATTAAATGGTCATCAGATAATGAACATGTCTGGCCATACTGCAACTGTTTGTTCAGTTGATTCACATGCATCAGGACTTATTGTCAGTGGTAGCGAAGATCGTTTGGCAAAATATGGAAAGTTTGTTTATTTTAGAAGAAAAGAAAATGGTCACAAGTTTTGTTTCGTATATCATATGAATAGACCAGTCTCTTCCATTATCACCGATCGAATTGACGTGCATGTGAAGCATCCAGGAATCAGTTTGTGTTCAGAGCATAGAGCATCCTGGTTTTGTTTGGGATACAAAATTTATGGAAAACGGGATATTGTGACAGCATGTTCCGATGGAGTTGTACGCATTTGGACTGTAAATCAGAATCATTTTGCTGGCCAGCGAGAACTTGATTTATACACTTCGCAACTTTCTATTAACAAGTCAATGCACAAAAAAGGAAATTGGATCGTAATTCCTTAA
Protein sequence

>MsG0480021403.01.T01

MIRLGQSRRRPIVKTLDHESEVTGIVVDGDDLVSSSIDGDLFGDKVLGGKEPTQRIISVAEMRMLCWILVWASSKRLCRFFNTVCGFAAMSDFGILSASHDGSLRLWALNGHQIMNMSGHTATVCSVDSHASGLIVSGSEDRLAKYGKFVYFRRKENGHKFCFVYHMNRPVSSIITDRIDVHVKHPGISLCSEHRASWFCLGYKIYGKRDIVTACSDGVVRIWTVNQNHFAGQRELDLYTSQLSINKSMHKKGNWIVIP*