AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021405.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021405.01.T01 MTR_4g069090 58.427 267 64 7 5 242 72 320 2.88e-87 274
MsG0480021405.01.T01 MTR_4g119620 30.097 206 115 13 16 207 136 326 2.71e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021405.01.T01 AT3G18860 46.816 267 95 7 5 242 75 323 2.01e-64 214
MsG0480021405.01.T01 AT3G18860 46.816 267 95 7 5 242 75 323 2.05e-64 214
MsG0480021405.01.T01 AT3G49660 29.442 197 116 12 16 202 129 312 4.01e-12 65.5

Find 60 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTACAGTTCTCTCAGATTA+TGG 0.178467 4:-57520950 MsG0480021405.01.T01:intron
AGAGTCCCTTGAACCTAAAA+TGG 0.203614 4:+57521485 None:intergenic
TTGGCTGCGATGTCTGATTT+TGG 0.214527 4:-57521093 MsG0480021405.01.T01:CDS
TTGAAAAGCGGTGGTGTTTC+TGG 0.222387 4:-57522416 MsG0480021405.01.T01:CDS
ATTTGAAGAGGTGGAGTAAT+TGG 0.238721 4:-57521743 MsG0480021405.01.T01:CDS
CTCAGATTATGGGCATTAAA+TGG 0.298081 4:-57520939 MsG0480021405.01.T01:CDS
TGAATAATGCTGTGGTTGTT+TGG 0.299775 4:-57522388 MsG0480021405.01.T01:CDS
AGCTTTATAACTGCTTGAAT+TGG 0.341084 4:+57521678 None:intergenic
CCGATGGAGTTGTACGCATT+TGG 0.342372 4:-57519492 MsG0480021405.01.T01:CDS
AATTACTGCACAAGAAATTC+AGG 0.352685 4:+57521763 None:intergenic
CCCACAGGAGAGCTTGTTAC+AGG 0.356136 4:-57521654 MsG0480021405.01.T01:intron
TCTTGTTCATCTTCTATTGA+TGG 0.361299 4:-57522288 MsG0480021405.01.T01:CDS
CTACAGTTCTCTCAGATTAT+GGG 0.367037 4:-57520949 MsG0480021405.01.T01:intron
AGCACCGTCCGATGTATATC+AGG 0.373422 4:-57522457 None:intergenic
GATCATGAATCGGAGGTTAC+TGG 0.380364 4:-57522332 MsG0480021405.01.T01:CDS
CAGTGGTAGCGAAGATCGTT+TGG 0.399445 4:-57520841 MsG0480021405.01.T01:CDS
GTTACTGGTATTGTGGTTGA+TGG 0.414174 4:-57522317 MsG0480021405.01.T01:CDS
GAATAATGCTGTGGTTGTTT+GGG 0.416958 4:-57522387 MsG0480021405.01.T01:CDS
GGGACTCTAGTTTAAAGATC+TGG 0.422473 4:-57521470 MsG0480021405.01.T01:CDS
GTTCGAATTGCACGGAGACA+TGG 0.436264 4:-57521716 MsG0480021405.01.T01:CDS
CACCATCATGGGATGCAGAT+AGG 0.445037 4:+57521067 None:intergenic
CTGAACAAACAGTTGCAGTA+TGG 0.447329 4:+57520892 None:intergenic
TTCATCTTCTATTGATGGGT+AGG 0.448207 4:-57522283 MsG0480021405.01.T01:CDS
TCAGTTGATTCACATGCATC+AGG 0.449667 4:-57520873 MsG0480021405.01.T01:CDS
CTTGTTCATCTTCTATTGAT+GGG 0.451237 4:-57522287 MsG0480021405.01.T01:CDS
TCATCTTCTATTGATGGGTA+GGG 0.472795 4:-57522282 MsG0480021405.01.T01:intron
TACAGATACTGTTTGTGGCT+TGG 0.485506 4:-57521112 MsG0480021405.01.T01:intron
CATCAGATAATGAACATGTC+TGG 0.498451 4:-57520915 MsG0480021405.01.T01:CDS
GAGTCCCTTGAACCTAAAAT+GGG 0.507826 4:+57521486 None:intergenic
CAGAAATGAACCCACCATCA+TGG 0.520627 4:+57521055 None:intergenic
GTTGTTTGGGACGTGGATAG+TGG 0.528762 4:-57522374 MsG0480021405.01.T01:CDS
GGAGACATGGGCGGCTCACG+AGG 0.534589 4:-57521703 MsG0480021405.01.T01:CDS
TGTGCAGTAATTTGAAGAGG+TGG 0.537765 4:-57521752 MsG0480021405.01.T01:CDS
TGCTGTGGTTGTTTGGGACG+TGG 0.540919 4:-57522381 MsG0480021405.01.T01:CDS
TTGGAATTGTTCGAATTGCA+CGG 0.545602 4:-57521724 MsG0480021405.01.T01:CDS
ACCTGTAACAAGCTCTCCTG+TGG 0.546432 4:+57521653 None:intergenic
GAATGTACAGATACTGTTTG+TGG 0.547131 4:-57521117 MsG0480021405.01.T01:intron
AGAAATGAACCCACCATCAT+GGG 0.549914 4:+57521056 None:intergenic
TTCGAATTGCACGGAGACAT+GGG 0.566298 4:-57521715 MsG0480021405.01.T01:CDS
GAAGACACTAGATCATGAAT+CGG 0.571511 4:-57522342 MsG0480021405.01.T01:CDS
TATCTGCATCCCATGATGGT+GGG 0.585315 4:-57521065 MsG0480021405.01.T01:intron
TCTTGTGCAGTAATTTGAAG+AGG 0.591310 4:-57521755 MsG0480021405.01.T01:CDS
CTATCTGCATCCCATGATGG+TGG 0.598808 4:-57521066 MsG0480021405.01.T01:CDS
ATCCTATCTGCATCCCATGA+TGG 0.605689 4:-57521069 MsG0480021405.01.T01:CDS
TGGTTGTGTGAATAATGCTG+TGG 0.608311 4:-57522396 MsG0480021405.01.T01:CDS
TGTATAAATCAAGTTCTCGC+TGG 0.611367 4:+57519443 None:intergenic
ATCGGAGGTTACTGGTATTG+TGG 0.622916 4:-57522324 MsG0480021405.01.T01:CDS
CCAAATGCGTACAACTCCAT+CGG 0.622939 4:+57519492 None:intergenic
CCTGTAACAAGCTCTCCTGT+GGG 0.625764 4:+57521654 None:intergenic
AACCCTACTTTGAAAAGCGG+TGG 0.631203 4:-57522425 MsG0480021405.01.T01:CDS
CTCTAGTTTAAAGATCTGGA+AGG 0.633553 4:-57521466 MsG0480021405.01.T01:CDS
ATTGTGACAGCATGTTCCGA+TGG 0.634535 4:-57519508 MsG0480021405.01.T01:intron
GACACTAGATCATGAATCGG+AGG 0.635591 4:-57522339 MsG0480021405.01.T01:CDS
GCAGTTATAAAGCTACCCAC+AGG 0.640686 4:-57521669 MsG0480021405.01.T01:CDS
TCTAGTTTAAAGATCTGGAA+GGG 0.642275 4:-57521465 MsG0480021405.01.T01:CDS
GCATCAGGACTTATTGTCAG+TGG 0.647307 4:-57520858 MsG0480021405.01.T01:CDS
CCGAACCCTACTTTGAAAAG+CGG 0.648212 4:-57522428 MsG0480021405.01.T01:CDS
GAGACATGGGCGGCTCACGA+GGG 0.670094 4:-57521702 MsG0480021405.01.T01:CDS
GGTGGAATCCTGATATACAT+CGG 0.695488 4:+57522449 None:intergenic
GAATTGCACGGAGACATGGG+CGG 0.723851 4:-57521712 MsG0480021405.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATGAATTTCTAAATT+TGG + Chr4:57521540-57521559 None:intergenic 10.0%
!! TCAATATTTATTAAATCTAG+AGG + Chr4:57520812-57520831 None:intergenic 15.0%
!! TGTTATTAATTCATATTAAG+AGG + Chr4:57520672-57520691 None:intergenic 15.0%
!!! ACTTTGTTTTCTTTATATTT+TGG - Chr4:57520516-57520535 MsG0480021405.01.T01:intron 15.0%
!!! ATTTTAGAAGAAAAAGAAAA+TGG - Chr4:57521340-57521359 MsG0480021405.01.T01:intron 15.0%
!!! CTTATTTCTATTTTATATTG+AGG - Chr4:57521292-57521311 MsG0480021405.01.T01:intron 15.0%
!! AAAACCACTATCAATAATTA+GGG + Chr4:57521935-57521954 None:intergenic 20.0%
!! AACTATAGAGATTAATGAAT+TGG - Chr4:57519836-57519855 MsG0480021405.01.T01:intron 20.0%
!! TAAAACCACTATCAATAATT+AGG + Chr4:57521936-57521955 None:intergenic 20.0%
!! TATGAATTTCTAAATTTGGT+AGG + Chr4:57521536-57521555 None:intergenic 20.0%
!! TTTGTCTTTATGAAGTTTAT+GGG - Chr4:57521155-57521174 MsG0480021405.01.T01:intron 20.0%
!!! AATAAACAAACTAGCTTATA+TGG + Chr4:57521323-57521342 None:intergenic 20.0%
!!! ATTATTGATAGTGGTTTTAT+TGG - Chr4:57521937-57521956 MsG0480021405.01.T01:intron 20.0%
!!! ATTTTGTCATCGATTTATAA+TGG - Chr4:57520465-57520484 MsG0480021405.01.T01:intron 20.0%
!!! GAAGATTTTTAGTAATCAAT+TGG - Chr4:57521960-57521979 MsG0480021405.01.T01:intron 20.0%
!!! GTTTTTTTTGCCTTTAATTT+CGG - Chr4:57522034-57522053 MsG0480021405.01.T01:intron 20.0%
!!! TGTTTTTTTTTTTTCTGTTC+TGG + Chr4:57521701-57521720 None:intergenic 20.0%
!!! TTATTGATAGTGGTTTTATT+GGG - Chr4:57521938-57521957 MsG0480021405.01.T01:intron 20.0%
!!! TTTATTTGTGATAAAGTGTT+GGG - Chr4:57519654-57519673 MsG0480021405.01.T01:intron 20.0%
!!! TTTTATTTGTGATAAAGTGT+TGG - Chr4:57519653-57519672 MsG0480021405.01.T01:intron 20.0%
!!! TTTTTTTTGCCTTTAATTTC+GGG - Chr4:57522035-57522054 MsG0480021405.01.T01:intron 20.0%
! AAAACAATTCATGTTGAAGA+AGG - Chr4:57521755-57521774 MsG0480021405.01.T01:CDS 25.0%
! AATCTGCTTGATTAACATTT+CGG - Chr4:57521241-57521260 MsG0480021405.01.T01:intron 25.0%
! AGAATAAGAAGAATGAATCA+CGG - Chr4:57522012-57522031 MsG0480021405.01.T01:intron 25.0%
! ATAAATAATTAACGTGTGAG+AGG - Chr4:57521839-57521858 MsG0480021405.01.T01:intron 25.0%
! ATTTCCCTAATTATTGATAG+TGG - Chr4:57521928-57521947 MsG0480021405.01.T01:intron 25.0%
! CTTTATCACAAATAAAATCG+CGG + Chr4:57519650-57519669 None:intergenic 25.0%
! GATACAAAATTTATGGAAAC+GGG - Chr4:57522300-57522319 MsG0480021405.01.T01:CDS 25.0%
! GCGAAAAAAACTAAAACTAT+GGG + Chr4:57522178-57522197 None:intergenic 25.0%
! GGATACAAAATTTATGGAAA+CGG - Chr4:57522299-57522318 MsG0480021405.01.T01:CDS 25.0%
! GTTTGTCTTTATGAAGTTTA+TGG - Chr4:57521154-57521173 MsG0480021405.01.T01:intron 25.0%
! TAAAATGTGTGTCTAAAGAT+GGG + Chr4:57520438-57520457 None:intergenic 25.0%
! TAAATAATTAACGTGTGAGA+GGG - Chr4:57521840-57521859 MsG0480021405.01.T01:intron 25.0%
! TTAAAATGTGTGTCTAAAGA+TGG + Chr4:57520439-57520458 None:intergenic 25.0%
! TTGTCTTTATGAAGTTTATG+GGG - Chr4:57521156-57521175 MsG0480021405.01.T01:intron 25.0%
!! AACTTATATTCTCCCATTTT+AGG - Chr4:57520317-57520336 MsG0480021405.01.T01:intron 25.0%
!! ATTCTTCTTATTCTCTGAAA+CGG + Chr4:57522006-57522025 None:intergenic 25.0%
!! GTTTGTTTACATACTTTTCA+TGG - Chr4:57520395-57520414 MsG0480021405.01.T01:intron 25.0%
!! TAACTTATAGCTCTTTTGTT+TGG - Chr4:57520489-57520508 MsG0480021405.01.T01:intron 25.0%
!! TGTTTGGGATACAAAATTTA+TGG - Chr4:57522293-57522312 MsG0480021405.01.T01:CDS 25.0%
!!! AAAAACGTTTTGAATCAATC+CGG - Chr4:57522058-57522077 MsG0480021405.01.T01:intron 25.0%
!!! AACTTTTCATTACCACTTTT+GGG - Chr4:57520223-57520242 MsG0480021405.01.T01:intron 25.0%
!!! TACAGGTATTGATACATTTT+CGG - Chr4:57520196-57520215 MsG0480021405.01.T01:intron 25.0%
AACAAGTCAATGCACAAAAA+AGG - Chr4:57522430-57522449 MsG0480021405.01.T01:CDS 30.0%
AAGATCGTTTGGCAAAAATA+TGG - Chr4:57521003-57521022 MsG0480021405.01.T01:intron 30.0%
AAGCTTCAAAATCATCTCAA+TGG - Chr4:57521725-57521744 MsG0480021405.01.T01:CDS 30.0%
AATTACTGCACAAGAAATTC+AGG + Chr4:57520073-57520092 None:intergenic 30.0%
ATAGCAATAATGTTTCCTTG+TGG - Chr4:57520572-57520591 MsG0480021405.01.T01:intron 30.0%
ATATGTGAATTGCATACGTA+AGG - Chr4:57521878-57521897 MsG0480021405.01.T01:intron 30.0%
ATGATACACTTCTGAATGTT+AGG - Chr4:57519994-57520013 MsG0480021405.01.T01:intron 30.0%
ATTAATTCTTAAGCTCCCTA+AGG + Chr4:57521130-57521149 None:intergenic 30.0%
ATTGTAATCTGCAGAACTAA+TGG - Chr4:57521804-57521823 MsG0480021405.01.T01:intron 30.0%
CAATGCACAAAAAAGGAAAT+TGG - Chr4:57522437-57522456 MsG0480021405.01.T01:CDS 30.0%
CTTGTTCATCTTCTATTGAT+GGG - Chr4:57519546-57519565 MsG0480021405.01.T01:intron 30.0%
GAAATAAGTTGCAATAACAG+CGG + Chr4:57521280-57521299 None:intergenic 30.0%
GGCGAAAAAAACTAAAACTA+TGG + Chr4:57522179-57522198 None:intergenic 30.0%
GTCATAGTCACATTAAAAGA+AGG - Chr4:57521777-57521796 MsG0480021405.01.T01:intron 30.0%
GTTGTGTAGATTCTTTAGTA+AGG - Chr4:57519760-57519779 MsG0480021405.01.T01:intron 30.0%
TACACTTATTATTCTCGTGT+TGG + Chr4:57519690-57519709 None:intergenic 30.0%
TAGTCACATTAAAAGAAGGA+AGG - Chr4:57521781-57521800 MsG0480021405.01.T01:intron 30.0%
TATGGGCCATGTAAATAAAA+CGG + Chr4:57522161-57522180 None:intergenic 30.0%
TCCAATACATCCATAAATCA+TGG + Chr4:57521906-57521925 None:intergenic 30.0%
TCCATGATTTATGGATGTAT+TGG - Chr4:57521902-57521921 MsG0480021405.01.T01:intron 30.0%
TCTTGTTCATCTTCTATTGA+TGG - Chr4:57519545-57519564 MsG0480021405.01.T01:intron 30.0%
TGTCTTTATGAAGTTTATGG+GGG - Chr4:57521157-57521176 MsG0480021405.01.T01:intron 30.0%
TTGTAATCTGCAGAACTAAT+GGG - Chr4:57521805-57521824 MsG0480021405.01.T01:intron 30.0%
! AACGTTTTTCCCGAAATTAA+AGG + Chr4:57522047-57522066 None:intergenic 30.0%
! AAGCAGATTCATACAACTTT+TGG + Chr4:57521230-57521249 None:intergenic 30.0%
! AGCTTTATAACTGCTTGAAT+TGG + Chr4:57520158-57520177 None:intergenic 30.0%
! CAACTTTTCATTACCACTTT+TGG - Chr4:57520222-57520241 MsG0480021405.01.T01:intron 30.0%
! GTAAATCAGAATCATTTTGC+TGG - Chr4:57522367-57522386 MsG0480021405.01.T01:CDS 30.0%
! TATATTCTTTTATGCTGCCA+GGG - Chr4:57520616-57520635 MsG0480021405.01.T01:intron 30.0%
! TCTAGTTTAAAGATCTGGAA+GGG - Chr4:57520368-57520387 MsG0480021405.01.T01:intron 30.0%
! TTATATTCTTTTATGCTGCC+AGG - Chr4:57520615-57520634 MsG0480021405.01.T01:intron 30.0%
!! AAAACGTTTTGAATCAATCC+GGG - Chr4:57522059-57522078 MsG0480021405.01.T01:intron 30.0%
!!! GTTCTTTTTTAGTGTCCTTA+GGG - Chr4:57521112-57521131 MsG0480021405.01.T01:intron 30.0%
!!! TGTTCTTTTTTAGTGTCCTT+AGG - Chr4:57521111-57521130 MsG0480021405.01.T01:intron 30.0%
AATGGGACTTGAACCTAAAA+TGG + Chr4:57520333-57520352 None:intergenic 35.0%
ACGTAAGGTTCCATGATTTA+TGG - Chr4:57521893-57521912 MsG0480021405.01.T01:intron 35.0%
ACTACAGTTCTCTCAGATTA+TGG - Chr4:57520883-57520902 MsG0480021405.01.T01:CDS 35.0%
AGTATCTGTACATTCGTCAA+AGG + Chr4:57520711-57520730 None:intergenic 35.0%
AGTCAAATTACCAGAGACAT+AGG - Chr4:57519801-57519820 MsG0480021405.01.T01:intron 35.0%
ATGGGACTTGAACCTAAAAT+GGG + Chr4:57520332-57520351 None:intergenic 35.0%
ATTTGAAGAGGTGGAGTAAT+TGG - Chr4:57520090-57520109 MsG0480021405.01.T01:intron 35.0%
CACTCAGAATTATGTCTCTT+AGG - Chr4:57520249-57520268 MsG0480021405.01.T01:intron 35.0%
CAGTCCAATACCATTTCAAA+TGG + Chr4:57520284-57520303 None:intergenic 35.0%
CATCAGATAATGAACATGTC+TGG - Chr4:57520918-57520937 MsG0480021405.01.T01:CDS 35.0%
CCTTTATGTCCTAAAATCGA+AGG + Chr4:57520548-57520567 None:intergenic 35.0%
CGTTTGGCAAAAATATGGAA+AGG - Chr4:57521008-57521027 MsG0480021405.01.T01:intron 35.0%
CTACAGTTCTCTCAGATTAT+GGG - Chr4:57520884-57520903 MsG0480021405.01.T01:CDS 35.0%
CTCAATACTGTTGAGTTTCA+TGG - Chr4:57521084-57521103 MsG0480021405.01.T01:CDS 35.0%
CTCAGATTATGGGCATTAAA+TGG - Chr4:57520894-57520913 MsG0480021405.01.T01:CDS 35.0%
GAAGACACTAGATCATGAAT+CGG - Chr4:57519491-57519510 MsG0480021405.01.T01:CDS 35.0%
GAAGTTCACACCATTTGAAA+TGG - Chr4:57520271-57520290 MsG0480021405.01.T01:intron 35.0%
GAATAATGCTGTGGTTGTTT+GGG - Chr4:57519446-57519465 MsG0480021405.01.T01:CDS 35.0%
GAATGTACAGATACTGTTTG+TGG - Chr4:57520716-57520735 MsG0480021405.01.T01:intron 35.0%
GGGAGCTTAAGAATTAATCT+TGG - Chr4:57521132-57521151 MsG0480021405.01.T01:intron 35.0%
GTCTTTATGAAGTTTATGGG+GGG - Chr4:57521158-57521177 MsG0480021405.01.T01:intron 35.0%
TAATAAGTGTAGCTGAGATG+AGG - Chr4:57519699-57519718 MsG0480021405.01.T01:intron 35.0%
TCTTGTGCAGTAATTTGAAG+AGG - Chr4:57520078-57520097 MsG0480021405.01.T01:intron 35.0%
TGAATAATGCTGTGGTTGTT+TGG - Chr4:57519445-57519464 MsG0480021405.01.T01:CDS 35.0%
TGCTACTGCTAATGAATTTG+AGG + Chr4:57521627-57521646 None:intergenic 35.0%
TGTATAAATCAAGTTCTCGC+TGG + Chr4:57522393-57522412 None:intergenic 35.0%
TGTCATTCCATTCCTTATTC+CGG - Chr4:57521204-57521223 MsG0480021405.01.T01:intron 35.0%
TTAAAGTCTTCCTATGTCTC+TGG + Chr4:57519814-57519833 None:intergenic 35.0%
TTGGAATTGTTCGAATTGCA+CGG - Chr4:57520109-57520128 MsG0480021405.01.T01:intron 35.0%
TTTGTATCCCAAACAAAACC+AGG + Chr4:57522288-57522307 None:intergenic 35.0%
! AGATTCATACAACTTTTGGC+CGG + Chr4:57521226-57521245 None:intergenic 35.0%
! CACACCATTTGAAATGGTAT+TGG - Chr4:57520277-57520296 MsG0480021405.01.T01:intron 35.0%
! CTCTAGTTTAAAGATCTGGA+AGG - Chr4:57520367-57520386 MsG0480021405.01.T01:intron 35.0%
! TCATCTTCTATTGATGGGTA+GGG - Chr4:57519551-57519570 MsG0480021405.01.T01:intron 35.0%
! TTAGGTTCAAGTCCCATTTT+AGG - Chr4:57520335-57520354 MsG0480021405.01.T01:intron 35.0%
! TTCATCTTCTATTGATGGGT+AGG - Chr4:57519550-57519569 MsG0480021405.01.T01:intron 35.0%
! TTTCCTTGTGGACATGATTT+TGG - Chr4:57520584-57520603 MsG0480021405.01.T01:intron 35.0%
!! ATTTGTGATAAAGTGTTGGG+TGG - Chr4:57519657-57519676 MsG0480021405.01.T01:intron 35.0%
!! TTTAACTTTTAGGCACTCGA+AGG - Chr4:57522132-57522151 MsG0480021405.01.T01:intron 35.0%
!!! AAGTCCCATTTTAGGTTCAA+GGG - Chr4:57520343-57520362 MsG0480021405.01.T01:intron 35.0%
!!! AGGCATCCGTTTTATTTACA+TGG - Chr4:57522152-57522171 MsG0480021405.01.T01:intron 35.0%
!!! CATCCAGGGTTTTAACTTTT+AGG - Chr4:57522122-57522141 MsG0480021405.01.T01:intron 35.0%
!!! CCTTCGATTTTAGGACATAA+AGG - Chr4:57520545-57520564 MsG0480021405.01.T01:intron 35.0%
AAGTTAAAACCCTGGATGCA+AGG + Chr4:57522120-57522139 None:intergenic 40.0%
ACAAACTCGAGATCCTAAGT+AGG - Chr4:57521455-57521474 MsG0480021405.01.T01:CDS 40.0%
AGAAATGAACCCACCATCAT+GGG + Chr4:57520780-57520799 None:intergenic 40.0%
AGAGTCCCTTGAACCTAAAA+TGG + Chr4:57520351-57520370 None:intergenic 40.0%
CTGAACAAACAGTTGCAGTA+TGG + Chr4:57520944-57520963 None:intergenic 40.0%
GAGTCCCTTGAACCTAAAAT+GGG + Chr4:57520350-57520369 None:intergenic 40.0%
GTAACAGAAACAAGTCAAGC+AGG + Chr4:57520865-57520884 None:intergenic 40.0%
GTCAATTCGATCGGTGATAA+TGG + Chr4:57521407-57521426 None:intergenic 40.0%
GTGCCTAAAAGTTAAAACCC+TGG + Chr4:57522128-57522147 None:intergenic 40.0%
GTTACTGGTATTGTGGTTGA+TGG - Chr4:57519516-57519535 MsG0480021405.01.T01:intron 40.0%
GTTGCAATAACAGCGGATAT+TGG + Chr4:57521273-57521292 None:intergenic 40.0%
TACAGATACTGTTTGTGGCT+TGG - Chr4:57520721-57520740 MsG0480021405.01.T01:intron 40.0%
TGGTTGTGTGAATAATGCTG+TGG - Chr4:57519437-57519456 MsG0480021405.01.T01:CDS 40.0%
TGTGCAGTAATTTGAAGAGG+TGG - Chr4:57520081-57520100 MsG0480021405.01.T01:intron 40.0%
TTCTGAGTGTAACCCAAAAG+TGG + Chr4:57520238-57520257 None:intergenic 40.0%
! CATACTTTTCATGGACATGC+AGG - Chr4:57520404-57520423 MsG0480021405.01.T01:intron 40.0%
! GGGACTCTAGTTTAAAGATC+TGG - Chr4:57520363-57520382 MsG0480021405.01.T01:intron 40.0%
! TACAACTTTTGGCCGGAATA+AGG + Chr4:57521219-57521238 None:intergenic 40.0%
! TCAGTTGATTCACATGCATC+AGG - Chr4:57520960-57520979 MsG0480021405.01.T01:intron 40.0%
!!! AGAGCATCCTGGTTTTGTTT+GGG - Chr4:57522278-57522297 MsG0480021405.01.T01:intron 40.0%
!!! AGTTTTTTTCGCCACATCAC+AGG - Chr4:57522186-57522205 MsG0480021405.01.T01:intron 40.0%
!!! ATGGGGGGATTTTTTGTTGT+GGG - Chr4:57521173-57521192 MsG0480021405.01.T01:intron 40.0%
!!! CAAGTCCCATTTTAGGTTCA+AGG - Chr4:57520342-57520361 MsG0480021405.01.T01:intron 40.0%
!!! TAGAGCATCCTGGTTTTGTT+TGG - Chr4:57522277-57522296 MsG0480021405.01.T01:intron 40.0%
!!! TATGGGGGGATTTTTTGTTG+TGG - Chr4:57521172-57521191 MsG0480021405.01.T01:intron 40.0%
ACCAAGCTTTACCACGAATC+AGG + Chr4:57519916-57519935 None:intergenic 45.0%
ACCAATGAGATCGTGAATCG+GGG - Chr4:57521488-57521507 MsG0480021405.01.T01:CDS 45.0%
AGCAACAAGTTCCTGTGATG+TGG + Chr4:57522200-57522219 None:intergenic 45.0%
AGTTTGTCTACCTTGCATCC+AGG - Chr4:57522107-57522126 MsG0480021405.01.T01:intron 45.0%
ATCCTATCTGCATCCCATGA+TGG - Chr4:57520764-57520783 MsG0480021405.01.T01:intron 45.0%
ATCGGAGGTTACTGGTATTG+TGG - Chr4:57519509-57519528 MsG0480021405.01.T01:intron 45.0%
ATTGTGACAGCATGTTCCGA+TGG - Chr4:57522325-57522344 MsG0480021405.01.T01:CDS 45.0%
CACATGCACGTCAATTCGAT+CGG + Chr4:57521416-57521435 None:intergenic 45.0%
CACCAATGAGATCGTGAATC+GGG - Chr4:57521487-57521506 MsG0480021405.01.T01:CDS 45.0%
CAGAAATGAACCCACCATCA+TGG + Chr4:57520781-57520800 None:intergenic 45.0%
CAGCAAAAATATGCAGTCCC+TGG + Chr4:57520636-57520655 None:intergenic 45.0%
CCAAATGCGTACAACTCCAT+CGG + Chr4:57522344-57522363 None:intergenic 45.0%
CCAAGCTTTACCACGAATCA+GGG + Chr4:57519915-57519934 None:intergenic 45.0%
CTCGAGATCCTAAGTAGGAT+CGG - Chr4:57521460-57521479 MsG0480021405.01.T01:CDS 45.0%
CTGCCAAAATCATGTCCACA+AGG + Chr4:57520590-57520609 None:intergenic 45.0%
GACACTAGATCATGAATCGG+AGG - Chr4:57519494-57519513 MsG0480021405.01.T01:CDS 45.0%
GATCATGAATCGGAGGTTAC+TGG - Chr4:57519501-57519520 MsG0480021405.01.T01:CDS 45.0%
GCACCAATGAGATCGTGAAT+CGG - Chr4:57521486-57521505 MsG0480021405.01.T01:CDS 45.0%
GCAGTTATAAAGCTACCCAC+AGG - Chr4:57520164-57520183 MsG0480021405.01.T01:intron 45.0%
GCATCAGGACTTATTGTCAG+TGG - Chr4:57520975-57520994 MsG0480021405.01.T01:intron 45.0%
GGTCGGCTACATGGATAAAA+CGG + Chr4:57519894-57519913 None:intergenic 45.0%
GTATTTCCTGATGCAGAGCA+AGG + Chr4:57520838-57520857 None:intergenic 45.0%
GTTGTGCTGGATCTTGGTTT+GGG - Chr4:57519724-57519743 MsG0480021405.01.T01:intron 45.0%
GTTTGTCTACCTTGCATCCA+GGG - Chr4:57522108-57522127 MsG0480021405.01.T01:intron 45.0%
TATCTGCATCCCATGATGGT+GGG - Chr4:57520768-57520787 MsG0480021405.01.T01:intron 45.0%
TGTTGTGCTGGATCTTGGTT+TGG - Chr4:57519723-57519742 MsG0480021405.01.T01:intron 45.0%
TTCGAATTGCACGGAGACAT+GGG - Chr4:57520118-57520137 MsG0480021405.01.T01:intron 45.0%
TTGAAAAGCGGTGGTGTTTC+TGG - Chr4:57519417-57519436 MsG0480021405.01.T01:CDS 45.0%
! AACCCTACTTTGAAAAGCGG+TGG - Chr4:57519408-57519427 MsG0480021405.01.T01:CDS 45.0%
! AGTAAGGAGAGCACAGATGA+AGG - Chr4:57519776-57519795 MsG0480021405.01.T01:intron 45.0%
! CCGAACCCTACTTTGAAAAG+CGG - Chr4:57519405-57519424 MsG0480021405.01.T01:CDS 45.0%
! CTGCAGAACTAATGGGTAGA+AGG - Chr4:57521812-57521831 MsG0480021405.01.T01:intron 45.0%
! CTTTTCAAAGTAGGGTTCGG+TGG + Chr4:57519405-57519424 None:intergenic 45.0%
! CTTTTGGCCGGAATAAGGAA+TGG + Chr4:57521214-57521233 None:intergenic 45.0%
! TGGTCTGTGCCTTCGATTTT+AGG - Chr4:57520536-57520555 MsG0480021405.01.T01:intron 45.0%
! TTGGCTGCGATGTCTGATTT+TGG - Chr4:57520740-57520759 MsG0480021405.01.T01:intron 45.0%
!! ACACCACCGCTTTTCAAAGT+AGG + Chr4:57519414-57519433 None:intergenic 45.0%
!! CACCACCGCTTTTCAAAGTA+GGG + Chr4:57519413-57519432 None:intergenic 45.0%
!! CATCACAGGAACTTGTTGCT+TGG - Chr4:57522200-57522219 MsG0480021405.01.T01:intron 45.0%
!! CCGCTTTTCAAAGTAGGGTT+CGG + Chr4:57519408-57519427 None:intergenic 45.0%
!! TCGGTGATAATGGAAGAGAC+TGG + Chr4:57521397-57521416 None:intergenic 45.0%
!!! ATTTTTTGTTGTGGGGTGGG+GGG - Chr4:57521181-57521200 MsG0480021405.01.T01:intron 45.0%
!!! GATTTTTTGTTGTGGGGTGG+GGG - Chr4:57521180-57521199 MsG0480021405.01.T01:intron 45.0%
!!! GGATTTTTTGTTGTGGGGTG+GGG - Chr4:57521179-57521198 MsG0480021405.01.T01:intron 45.0%
!!! GGGATTTTTTGTTGTGGGGT+GGG - Chr4:57521178-57521197 MsG0480021405.01.T01:intron 45.0%
!!! TGGGGGGATTTTTTGTTGTG+GGG - Chr4:57521174-57521193 MsG0480021405.01.T01:intron 45.0%
AAACTCGCGATCCAACTCGT+AGG + Chr4:57522092-57522111 None:intergenic 50.0%
ACCTGTAACAAGCTCTCCTG+TGG + Chr4:57520183-57520202 None:intergenic 50.0%
CACCATCATGGGATGCAGAT+AGG + Chr4:57520769-57520788 None:intergenic 50.0%
CCGATGGAGTTGTACGCATT+TGG - Chr4:57522341-57522360 MsG0480021405.01.T01:CDS 50.0%
CCTGTAACAAGCTCTCCTGT+GGG + Chr4:57520182-57520201 None:intergenic 50.0%
CTATCTGCATCCCATGATGG+TGG - Chr4:57520767-57520786 MsG0480021405.01.T01:intron 50.0%
CTGAGATGAGGATGTTGTGC+TGG - Chr4:57519711-57519730 MsG0480021405.01.T01:intron 50.0%
GAGGATGTTGTGCTGGATCT+TGG - Chr4:57519718-57519737 MsG0480021405.01.T01:intron 50.0%
GCTTTACCACGAATCAGGGT+CGG + Chr4:57519911-57519930 None:intergenic 50.0%
GTGAATCGGGGATCGTAACA+CGG - Chr4:57521500-57521519 MsG0480021405.01.T01:intron 50.0%
GTTCGAATTGCACGGAGACA+TGG - Chr4:57520117-57520136 MsG0480021405.01.T01:intron 50.0%
TCCCCGATTCACGATCTCAT+TGG + Chr4:57521492-57521511 None:intergenic 50.0%
TCGAGTTTGTCTACCTTGCC+TGG + Chr4:57521445-57521464 None:intergenic 50.0%
TGAATGCCTTGCTCTGCATC+AGG - Chr4:57520829-57520848 MsG0480021405.01.T01:CDS 50.0%
! CAGTGGTAGCGAAGATCGTT+TGG - Chr4:57520992-57521011 MsG0480021405.01.T01:intron 50.0%
! CCCTGATTCGTGGTAAAGCT+TGG - Chr4:57519912-57519931 MsG0480021405.01.T01:intron 50.0%
! GGTGCTATCCGATCCTACTT+AGG + Chr4:57521471-57521490 None:intergenic 50.0%
! GTTCAGAGCATAGAGCATCC+TGG - Chr4:57522267-57522286 MsG0480021405.01.T01:intron 50.0%
! GTTGTTTGGGACGTGGATAG+TGG - Chr4:57519459-57519478 MsG0480021405.01.T01:CDS 50.0%
! TGGTTTGGGCAAGTAGCAAG+AGG - Chr4:57519738-57519757 MsG0480021405.01.T01:intron 50.0%
!!! GGGGATTTTTTGTTGTGGGG+TGG - Chr4:57521177-57521196 MsG0480021405.01.T01:intron 50.0%
ACGAATCAGGGTCGGCTACA+TGG + Chr4:57519903-57519922 None:intergenic 55.0%
CGATCCAACTCGTAGGATCG+TGG + Chr4:57522085-57522104 None:intergenic 55.0%
GAATTGCACGGAGACATGGG+CGG - Chr4:57520121-57520140 MsG0480021405.01.T01:intron 55.0%
! ATGTAGCCGACCCTGATTCG+TGG - Chr4:57519902-57519921 MsG0480021405.01.T01:intron 55.0%
! GACGTGCATGTGAAGCATCC+AGG - Chr4:57521424-57521443 MsG0480021405.01.T01:intron 55.0%
! GCATGTGAAGCATCCAGGCA+AGG - Chr4:57521429-57521448 MsG0480021405.01.T01:intron 55.0%
! TGCTGTGGTTGTTTGGGACG+TGG - Chr4:57519452-57519471 MsG0480021405.01.T01:CDS 55.0%
!! CCCACAGGAGAGCTTGTTAC+AGG - Chr4:57520179-57520198 MsG0480021405.01.T01:intron 55.0%
CAACTCGTAGGATCGTGGCC+CGG + Chr4:57522080-57522099 None:intergenic 60.0%
CGGGCCACGATCCTACGAGT+TGG - Chr4:57522078-57522097 MsG0480021405.01.T01:intron 65.0%
GAGACATGGGCGGCTCACGA+GGG - Chr4:57520131-57520150 MsG0480021405.01.T01:intron 65.0%
GGAGACATGGGCGGCTCACG+AGG - Chr4:57520130-57520149 MsG0480021405.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 57519387 57522468 57519387 ID=MsG0480021405.01;Name=MsG0480021405.01
Chr4 mRNA 57519387 57522468 57519387 ID=MsG0480021405.01.T01;Parent=MsG0480021405.01;Name=MsG0480021405.01.T01;_AED=0.13;_eAED=0.14;_QI=0|0.4|0.33|1|1|1|6|0|247
Chr4 exon 57519387 57519520 57519387 ID=MsG0480021405.01.T01:exon:17204;Parent=MsG0480021405.01.T01
Chr4 exon 57520826 57520965 57520826 ID=MsG0480021405.01.T01:exon:17203;Parent=MsG0480021405.01.T01
Chr4 exon 57521066 57521129 57521066 ID=MsG0480021405.01.T01:exon:17202;Parent=MsG0480021405.01.T01
Chr4 exon 57521430 57521516 57521430 ID=MsG0480021405.01.T01:exon:17201;Parent=MsG0480021405.01.T01
Chr4 exon 57521655 57521787 57521655 ID=MsG0480021405.01.T01:exon:17200;Parent=MsG0480021405.01.T01
Chr4 exon 57522283 57522468 57522283 ID=MsG0480021405.01.T01:exon:17199;Parent=MsG0480021405.01.T01
Chr4 CDS 57522283 57522468 57522283 ID=MsG0480021405.01.T01:cds;Parent=MsG0480021405.01.T01
Chr4 CDS 57521655 57521787 57521655 ID=MsG0480021405.01.T01:cds;Parent=MsG0480021405.01.T01
Chr4 CDS 57521430 57521516 57521430 ID=MsG0480021405.01.T01:cds;Parent=MsG0480021405.01.T01
Chr4 CDS 57521066 57521129 57521066 ID=MsG0480021405.01.T01:cds;Parent=MsG0480021405.01.T01
Chr4 CDS 57520826 57520965 57520826 ID=MsG0480021405.01.T01:cds;Parent=MsG0480021405.01.T01
Chr4 CDS 57519387 57519520 57519387 ID=MsG0480021405.01.T01:cds;Parent=MsG0480021405.01.T01
Gene Sequence

>MsG0480021405.01.T01

ATGTATATCAGGATTCCACCGAACCCTACTTTGAAAAGCGGTGGTGTTTCTGGTTGTGTGAATAATGCTGTGGTTGTTTGGGACGTGGATAGTGGTAAGATTGTGAAGACACTAGATCATGAATCGGAGGTTACTGGTATTGTGGTTGATGGAGATGATCTTGTTCATCTTCTATTGATGGGTAGGTGCCTGAATTTCTTGTGCAGTAATTTGAAGAGGTGGAGTAATTGGAATTGTTCGAATTGCACGGAGACATGGGCGGCTCACGAGGGTCCAATTCAAGCAGTTATAAAGCTACCCACAGGAGAGCTTGTTACAGGTTCAAGTCCCATTTTAGGTTCAAGGGACTCTAGTTTAAAGATCTGGAAGGGCAAAGTTTGTTTACATACTTTTCATGGACATGCAGATACTGTTTGTGGCTTGGCTGCGATGTCTGATTTTGGAATCCTATCTGCATCCCATGATGGTGGTTCTCTCAGATTATGGGCATTAAATGGTCATCAGATAATGAACATGTCTGGCCATACTGCAACTGTTTGTTCAGTTGATTCACATGCATCAGGACTTATTGTCAGTGGTAGCGAAGATCGTTTGGCAAAAATATGGAAAGCATGTTCCGATGGAGTTGTACGCATTTGGACTGTAAATCAGAATCATTTTGCTGGCCAGCGAGAACTTGATTTATACACTTCGCAACTTTCTATTAACAAGTCAATGCACAAAAAAGGAAATTGGATAACGTAA
Protein sequence

>MsG0480021405.01.T01

MYIRIPPNPTLKSGGVSGCVNNAVVVWDVDSGKIVKTLDHESEVTGIVVDGDDLVHLLLMGRCLNFLCSNLKRWSNWNCSNCTETWAAHEGPIQAVIKLPTGELVTGSSPILGSRDSSLKIWKGKVCLHTFHGHADTVCGLAAMSDFGILSASHDGGSLRLWALNGHQIMNMSGHTATVCSVDSHASGLIVSGSEDRLAKIWKACSDGVVRIWTVNQNHFAGQRELDLYTSQLSINKSMHKKGNWIT*