Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006510.01.T01 | XP_021727808.1 | 72.5 | 80 | 22 | 0 | 1 | 80 | 1 | 80 | 2.12E-33 | 122 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006510.01.T01 | A0A1S4C0A8 | 70.000 | 80 | 24 | 0 | 1 | 80 | 1 | 80 | 1.35e-31 | 118 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048874.01 | MsG0280006510.01 | 0.801289 | 9.659707e-49 | 4.595878e-46 |
| MsG0180000470.01 | MsG0280006510.01 | 0.803839 | 2.877795e-49 | 1.461195e-46 |
| MsG0180000585.01 | MsG0280006510.01 | 0.802182 | 6.333574e-49 | 3.082275e-46 |
| MsG0180002195.01 | MsG0280006510.01 | 0.815707 | 8.054118e-52 | 5.594193e-49 |
| MsG0180002952.01 | MsG0280006510.01 | 0.806601 | 7.598770e-50 | 4.141591e-47 |
| MsG0180004264.01 | MsG0280006510.01 | 0.809184 | 2.144765e-50 | 1.250791e-47 |
| MsG0180005445.01 | MsG0280006510.01 | 0.800351 | 1.501151e-48 | 6.974528e-46 |
| MsG0180005629.01 | MsG0280006510.01 | 0.814975 | 1.171284e-51 | 7.973903e-49 |
| MsG0180006134.01 | MsG0280006510.01 | 0.803454 | 3.459485e-49 | 1.739164e-46 |
| MsG0280006510.01 | MsG0280006692.01 | 0.809392 | 1.935874e-50 | 1.135235e-47 |
| MsG0280006510.01 | MsG0280008073.01 | 0.818122 | 2.310536e-52 | 1.714526e-49 |
| MsG0280006510.01 | MsG0280008270.01 | 0.801812 | 7.545943e-49 | 3.637971e-46 |
| MsG0280006510.01 | MsG0280008615.01 | 0.806962 | 6.375756e-50 | 3.507514e-47 |
| MsG0280006510.01 | MsG0280010102.01 | 0.829480 | 5.026643e-55 | 5.150891e-52 |
| MsG0280006510.01 | MsG0280010117.01 | 0.811118 | 8.213125e-51 | 5.041256e-48 |
| MsG0280006510.01 | MsG0280010443.01 | 0.801257 | 9.808026e-49 | 4.662633e-46 |
| MsG0280006510.01 | MsG0280010614.01 | 0.809234 | 2.092301e-50 | 1.221853e-47 |
| MsG0280006510.01 | MsG0280010698.01 | 0.809095 | 2.240957e-50 | 1.303775e-47 |
| MsG0280006510.01 | MsG0380012021.01 | 0.801483 | 8.814338e-49 | 4.214214e-46 |
| MsG0280006510.01 | MsG0380012033.01 | 0.803956 | 2.721444e-49 | 1.385981e-46 |
| MsG0280006510.01 | MsG0380012330.01 | 0.801332 | 9.465884e-49 | 4.508447e-46 |
| MsG0280006510.01 | MsG0380012365.01 | 0.802435 | 5.616863e-49 | 2.751301e-46 |
| MsG0280006510.01 | MsG0380013176.01 | 0.823142 | 1.624091e-53 | 1.386845e-50 |
| MsG0280006510.01 | MsG0380014122.01 | 0.866210 | 3.368000e-65 | 1.125970e-61 |
| MsG0280006510.01 | MsG0380014135.01 | 0.829411 | 5.226863e-55 | 5.345569e-52 |
| MsG0280006510.01 | MsG0380014573.01 | 0.804523 | 2.073640e-49 | 1.071470e-46 |
| MsG0280006510.01 | MsG0380014894.01 | 0.829836 | 4.119119e-55 | 4.265750e-52 |
| MsG0280006510.01 | MsG0380014902.01 | 0.807205 | 5.662976e-50 | 3.135180e-47 |
| MsG0280006510.01 | MsG0380015038.01 | 0.804409 | 2.190227e-49 | 1.128422e-46 |
| MsG0280006510.01 | MsG0380015877.01 | 0.803973 | 2.699448e-49 | 1.375352e-46 |
| MsG0280006510.01 | MsG0380016909.01 | 0.806148 | 9.468813e-50 | 5.101528e-47 |
| MsG0280006510.01 | MsG0480019435.01 | 0.800627 | 1.318969e-48 | 6.171041e-46 |
| MsG0280006510.01 | MsG0480021403.01 | 0.812860 | 3.428324e-51 | 2.203982e-48 |
| MsG0280006510.01 | MsG0480021405.01 | 0.800760 | 1.238858e-48 | 5.815617e-46 |
| MsG0280006510.01 | MsG0480021688.01 | 0.812990 | 3.210239e-51 | 2.071052e-48 |
| MsG0280006510.01 | MsG0480021775.01 | 0.832807 | 7.658583e-56 | 8.654331e-53 |
| MsG0280006510.01 | MsG0480021900.01 | 0.813057 | 3.103101e-51 | 2.005519e-48 |
| MsG0280006510.01 | MsG0480022862.01 | 0.863832 | 1.878098e-64 | 5.772701e-61 |
| MsG0280006510.01 | MsG0480023654.01 | 0.803722 | 3.044012e-49 | 1.540866e-46 |
| MsG0280006510.01 | MsG0480023670.01 | 0.805181 | 1.511218e-49 | 7.942480e-47 |
| MsG0280006510.01 | MsG0580024682.01 | 0.807664 | 4.525537e-50 | 2.535443e-47 |
| MsG0280006510.01 | MsG0580024690.01 | 0.828368 | 9.346506e-55 | 9.270935e-52 |
| MsG0280006510.01 | MsG0580024918.01 | 0.855868 | 4.725943e-62 | 1.106787e-58 |
| MsG0280006510.01 | MsG0580027548.01 | 0.802467 | 5.532478e-49 | 2.712132e-46 |
| MsG0280006510.01 | MsG0580027617.01 | 0.854226 | 1.417221e-61 | 3.143547e-58 |
| MsG0280006510.01 | MsG0580027833.01 | 0.802160 | 6.399185e-49 | 3.112556e-46 |
| MsG0280006510.01 | MsG0580027838.01 | 0.802245 | 6.147721e-49 | 2.996743e-46 |
| MsG0280006510.01 | MsG0580029101.01 | 0.863206 | 2.936372e-64 | 8.830985e-61 |
| MsG0280006510.01 | MsG0680030561.01 | 0.808026 | 3.790803e-50 | 2.144107e-47 |
| MsG0280006510.01 | MsG0680030677.01 | 0.806641 | 7.452356e-50 | 4.065761e-47 |
| MsG0280006510.01 | MsG0680035444.01 | 0.806137 | 9.520285e-50 | 5.127850e-47 |
| MsG0280006510.01 | MsG0680035573.01 | 0.801083 | 1.064614e-48 | 5.039030e-46 |
| MsG0280006510.01 | MsG0780036018.01 | 0.808115 | 3.629386e-50 | 2.057632e-47 |
| MsG0280006510.01 | MsG0780036080.01 | 0.801518 | 8.671975e-49 | 4.149797e-46 |
| MsG0280006510.01 | MsG0780036201.01 | 0.848866 | 4.662835e-60 | 8.677447e-57 |
| MsG0280006510.01 | MsG0780037146.01 | 0.804397 | 2.203422e-49 | 1.134851e-46 |
| MsG0280006510.01 | MsG0780038113.01 | 0.815332 | 9.762580e-52 | 6.711251e-49 |
| MsG0280006510.01 | MsG0780038401.01 | 0.806826 | 6.811205e-50 | 3.733949e-47 |
| MsG0280006510.01 | MsG0780038597.01 | 0.865440 | 5.894887e-65 | 1.918027e-61 |
| MsG0280006510.01 | MsG0780038691.01 | 0.804524 | 2.072717e-49 | 1.071029e-46 |
| MsG0280006510.01 | MsG0780039252.01 | 0.819801 | 9.595681e-53 | 7.459816e-50 |
| MsG0280006510.01 | MsG0780039503.01 | 0.815969 | 7.038457e-52 | 4.923844e-49 |
| MsG0280006510.01 | MsG0780039544.01 | 0.814774 | 1.298386e-51 | 8.790996e-49 |
| MsG0280006510.01 | MsG0780039655.01 | 0.826760 | 2.272681e-54 | 2.150976e-51 |
| MsG0280006510.01 | MsG0780040186.01 | 0.875243 | 3.593706e-68 | 1.666201e-64 |
| MsG0280006510.01 | MsG0780040377.01 | 0.803111 | 4.074774e-49 | 2.030455e-46 |
| MsG0280006510.01 | MsG0780040788.01 | 0.805906 | 1.064647e-49 | 5.700709e-47 |
| MsG0280006510.01 | MsG0780041764.01 | 0.805982 | 1.026054e-49 | 5.505052e-47 |
| MsG0280006510.01 | MsG0880042744.01 | 0.802958 | 4.380907e-49 | 2.174585e-46 |
| MsG0280006510.01 | MsG0880044257.01 | 0.804888 | 1.740041e-49 | 9.077534e-47 |
| MsG0280006510.01 | MsG0880044283.01 | 0.802243 | 6.153128e-49 | 2.999207e-46 |
| MsG0280006510.01 | MsG0880044748.01 | 0.814087 | 1.841303e-51 | 1.223790e-48 |
| MsG0280006510.01 | MsG0880045372.01 | 0.808185 | 3.505941e-50 | 1.991422e-47 |
| MsG0280006510.01 | MsG0880045639.01 | 0.811191 | 7.921862e-51 | 4.871749e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006510.01.T01 | MTR_7g062720 | 69.333 | 75 | 23 | 0 | 1 | 75 | 1 | 75 | 2.31e-34 | 115 |
| MsG0280006510.01.T01 | MTR_5g061150 | 65.000 | 60 | 21 | 0 | 20 | 79 | 10 | 69 | 1.21e-20 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGCTCTTAATATTTC+TGG | 0.080746 | 2:+2914684 | MsG0280006510.01.T01:CDS |
| TTCGGCATTTAAATGAATTT+TGG | 0.166319 | 2:-2914736 | None:intergenic |
| TAAATTGTCCCAAGATCTTT+TGG | 0.231375 | 2:-2914882 | None:intergenic |
| TATTTCTGGAAATAATTATC+TGG | 0.282933 | 2:+2914698 | MsG0280006510.01.T01:CDS |
| CTGGAAATAATTATCTGGCT+TGG | 0.347827 | 2:+2914703 | MsG0280006510.01.T01:CDS |
| TGGAAATAATTATCTGGCTT+GGG | 0.353242 | 2:+2914704 | MsG0280006510.01.T01:CDS |
| ATAGAAGATCCAAAAGATCT+TGG | 0.420939 | 2:+2914873 | MsG0280006510.01.T01:CDS |
| CCTTCGTCACCACCTTCATA+AGG | 0.433246 | 2:+2914827 | MsG0280006510.01.T01:CDS |
| CAAAACAACTGAACAACAAA+AGG | 0.454927 | 2:+2914788 | MsG0280006510.01.T01:CDS |
| AATTGTATCTCCATGCCCTT+CGG | 0.460742 | 2:-2914754 | None:intergenic |
| TAGAAGATCCAAAAGATCTT+GGG | 0.493474 | 2:+2914874 | MsG0280006510.01.T01:CDS |
| CATTTAAATGCCGAAGGGCA+TGG | 0.494088 | 2:+2914744 | MsG0280006510.01.T01:CDS |
| CATGGAGATACAATTAAAGA+AGG | 0.533844 | 2:+2914762 | MsG0280006510.01.T01:CDS |
| AAAATTCATTTAAATGCCGA+AGG | 0.537172 | 2:+2914738 | MsG0280006510.01.T01:CDS |
| AACTGAACAACAAAAGGCTA+AGG | 0.582581 | 2:+2914794 | MsG0280006510.01.T01:CDS |
| CCTTATGAAGGTGGTGACGA+AGG | 0.598722 | 2:-2914827 | None:intergenic |
| AAATTCATTTAAATGCCGAA+GGG | 0.631745 | 2:+2914739 | MsG0280006510.01.T01:CDS |
| GTGGTGACGAAGGAATATCA+TGG | 0.663314 | 2:-2914817 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTCTGGAAATAATTATC+TGG | + | Chr2:2914698-2914717 | MsG0280006510.01.T01:CDS | 20.0% |
| ! | AAAATTCATTTAAATGCCGA+AGG | + | Chr2:2914738-2914757 | MsG0280006510.01.T01:CDS | 25.0% |
| ! | AAATTCATTTAAATGCCGAA+GGG | + | Chr2:2914739-2914758 | MsG0280006510.01.T01:CDS | 25.0% |
| !! | TTCGGCATTTAAATGAATTT+TGG | - | Chr2:2914739-2914758 | None:intergenic | 25.0% |
| !!! | CATTTTTTAGATCCTTATGA+AGG | - | Chr2:2914842-2914861 | None:intergenic | 25.0% |
| ATAGAAGATCCAAAAGATCT+TGG | + | Chr2:2914873-2914892 | MsG0280006510.01.T01:CDS | 30.0% | |
| CAAAACAACTGAACAACAAA+AGG | + | Chr2:2914788-2914807 | MsG0280006510.01.T01:CDS | 30.0% | |
| CATGGAGATACAATTAAAGA+AGG | + | Chr2:2914762-2914781 | MsG0280006510.01.T01:CDS | 30.0% | |
| TAGAAGATCCAAAAGATCTT+GGG | + | Chr2:2914874-2914893 | MsG0280006510.01.T01:CDS | 30.0% | |
| ! | AAGATCTTTTGGATCTTCTA+TGG | - | Chr2:2914874-2914893 | None:intergenic | 30.0% |
| ! | TAAATTGTCCCAAGATCTTT+TGG | - | Chr2:2914885-2914904 | None:intergenic | 30.0% |
| ! | TGGAAATAATTATCTGGCTT+GGG | + | Chr2:2914704-2914723 | MsG0280006510.01.T01:CDS | 30.0% |
| ! | TTTGAGGCTCTTAATATTTC+TGG | + | Chr2:2914684-2914703 | MsG0280006510.01.T01:CDS | 30.0% |
| ! | TTTTTAGATCCTTATGAAGG+TGG | - | Chr2:2914839-2914858 | None:intergenic | 30.0% |
| AACTGAACAACAAAAGGCTA+AGG | + | Chr2:2914794-2914813 | MsG0280006510.01.T01:CDS | 35.0% | |
| CTGGAAATAATTATCTGGCT+TGG | + | Chr2:2914703-2914722 | MsG0280006510.01.T01:CDS | 35.0% | |
| ! | CCTCAAAATCAAGTTTTGCA+AGG | - | Chr2:2914671-2914690 | None:intergenic | 35.0% |
| ! | CCTTGCAAAACTTGATTTTG+AGG | + | Chr2:2914668-2914687 | MsG0280006510.01.T01:CDS | 35.0% |
| ! | CTTTTGGATCTTCTATGGTA+AGG | - | Chr2:2914869-2914888 | None:intergenic | 35.0% |
| AATTGTATCTCCATGCCCTT+CGG | - | Chr2:2914757-2914776 | None:intergenic | 40.0% | |
| CATTTAAATGCCGAAGGGCA+TGG | + | Chr2:2914744-2914763 | MsG0280006510.01.T01:CDS | 45.0% | |
| ! | GTGGTGACGAAGGAATATCA+TGG | - | Chr2:2914820-2914839 | None:intergenic | 45.0% |
| CCTTCGTCACCACCTTCATA+AGG | + | Chr2:2914827-2914846 | MsG0280006510.01.T01:CDS | 50.0% | |
| ! | CCTTATGAAGGTGGTGACGA+AGG | - | Chr2:2914830-2914849 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 2914660 | 2914905 | 2914660 | ID=MsG0280006510.01;Name=MsG0280006510.01 |
| Chr2 | mRNA | 2914660 | 2914905 | 2914660 | ID=MsG0280006510.01.T01;Parent=MsG0280006510.01;Name=MsG0280006510.01.T01;_AED=0.47;_eAED=0.51;_QI=0|-1|0|1|-1|1|1|0|81 |
| Chr2 | exon | 2914660 | 2914905 | 2914660 | ID=MsG0280006510.01.T01:exon:1565;Parent=MsG0280006510.01.T01 |
| Chr2 | CDS | 2914660 | 2914905 | 2914660 | ID=MsG0280006510.01.T01:cds;Parent=MsG0280006510.01.T01 |
| Gene Sequence |
| Protein sequence |
