Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021825.01.T01 | XP_003607369.1 | 74.576 | 59 | 11 | 3 | 10 | 65 | 113 | 170 | 1.09E-18 | 89.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021825.01.T01 | Q9C8J6 | 59.259 | 54 | 20 | 2 | 9 | 60 | 99 | 152 | 1.10E-13 | 66.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021825.01.T01 | G7JRN3 | 74.576 | 59 | 11 | 3 | 10 | 65 | 113 | 170 | 5.19e-19 | 89.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011401.01 | MsG0480021825.01 | 0.811110 | 8.248243e-51 | 5.061769e-48 |
| MsG0480021825.01 | MsG0480022617.01 | 0.815616 | 8.439802e-52 | 5.847871e-49 |
| MsG0480021825.01 | MsG0580024717.01 | 0.800600 | 1.335482e-48 | 6.244089e-46 |
| MsG0480021825.01 | MsG0680031977.01 | 0.806122 | 9.590751e-50 | 5.163861e-47 |
| MsG0480021825.01 | MsG0680032254.01 | 0.805458 | 1.322018e-49 | 6.997924e-47 |
| MsG0480021825.01 | MsG0680034028.01 | 0.809519 | 1.818175e-50 | 1.069802e-47 |
| MsG0480021825.01 | MsG0880044619.01 | 0.802740 | 4.860736e-49 | 2.399472e-46 |
| MsG0280009757.01 | MsG0480021825.01 | 0.800953 | 1.131262e-48 | 5.337045e-46 |
| MsG0280010257.01 | MsG0480021825.01 | 0.806294 | 8.821068e-50 | 4.770295e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021825.01.T01 | MTR_4g077070 | 74.576 | 59 | 11 | 3 | 10 | 65 | 113 | 170 | 1.32e-22 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021825.01.T01 | AT1G51440 | 59.259 | 54 | 20 | 2 | 9 | 60 | 99 | 152 | 1.12e-14 | 66.6 |
Find 16 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTCCCAGTGTTCTCGCTT+TGG | 0.338497 | 4:+64854929 | None:intergenic |
| GAGAAACTTTACATGGGAGA+TGG | 0.493049 | 4:-64854770 | MsG0480021825.01.T01:CDS |
| TTGCGTAGGATGGGATCCAT+TGG | 0.494207 | 4:+64854898 | None:intergenic |
| GAATGATTATTTGCGTAGGA+TGG | 0.496379 | 4:+64854888 | None:intergenic |
| AGAAACTTTACATGGGAGAT+GGG | 0.511378 | 4:-64854769 | MsG0480021825.01.T01:CDS |
| TTTCTTTGAGAAACTTTACA+TGG | 0.516670 | 4:-64854777 | MsG0480021825.01.T01:CDS |
| GAAATCCAAAGCGAGAACAC+TGG | 0.520034 | 4:-64854934 | MsG0480021825.01.T01:CDS |
| AATGATTATTTGCGTAGGAT+GGG | 0.530762 | 4:+64854889 | None:intergenic |
| TTTGATCAAACTCAAAATTG+TGG | 0.534733 | 4:-64854824 | MsG0480021825.01.T01:CDS |
| AAATCCAAAGCGAGAACACT+GGG | 0.545178 | 4:-64854933 | MsG0480021825.01.T01:CDS |
| AGTTTCTCAAAGAAATGTGC+AGG | 0.546930 | 4:+64854785 | None:intergenic |
| TGGGAAGGTCTAAGATCCAA+TGG | 0.587434 | 4:-64854914 | MsG0480021825.01.T01:CDS |
| ACGCAAATAATCATTCGCTA+TGG | 0.600910 | 4:-64854881 | MsG0480021825.01.T01:CDS |
| TAGCGAATGATTATTTGCGT+AGG | 0.612158 | 4:+64854884 | None:intergenic |
| TTCTTTGAGAAACTTTACAT+GGG | 0.642102 | 4:-64854776 | MsG0480021825.01.T01:CDS |
| CCAAAGCGAGAACACTGGGA+AGG | 0.651227 | 4:-64854929 | MsG0480021825.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGATCAAACTCAAAATTG+TGG | - | Chr4:64854877-64854896 | MsG0480021825.01.T01:CDS | 25.0% |
| !! | TTCTTTGAGAAACTTTACAT+GGG | - | Chr4:64854925-64854944 | MsG0480021825.01.T01:CDS | 25.0% |
| !! | TTTCTTTGAGAAACTTTACA+TGG | - | Chr4:64854924-64854943 | MsG0480021825.01.T01:CDS | 25.0% |
| AATGATTATTTGCGTAGGAT+GGG | + | Chr4:64854815-64854834 | None:intergenic | 30.0% | |
| ACGCAAATAATCATTCGCTA+TGG | - | Chr4:64854820-64854839 | MsG0480021825.01.T01:CDS | 35.0% | |
| AGAAACTTTACATGGGAGAT+GGG | - | Chr4:64854932-64854951 | MsG0480021825.01.T01:CDS | 35.0% | |
| AGTTTCTCAAAGAAATGTGC+AGG | + | Chr4:64854919-64854938 | None:intergenic | 35.0% | |
| GAATGATTATTTGCGTAGGA+TGG | + | Chr4:64854816-64854835 | None:intergenic | 35.0% | |
| TAGCGAATGATTATTTGCGT+AGG | + | Chr4:64854820-64854839 | None:intergenic | 35.0% | |
| AAATCCAAAGCGAGAACACT+GGG | - | Chr4:64854768-64854787 | MsG0480021825.01.T01:CDS | 40.0% | |
| GAGAAACTTTACATGGGAGA+TGG | - | Chr4:64854931-64854950 | MsG0480021825.01.T01:CDS | 40.0% | |
| GAAATCCAAAGCGAGAACAC+TGG | - | Chr4:64854767-64854786 | MsG0480021825.01.T01:CDS | 45.0% | |
| ! | TGGGAAGGTCTAAGATCCAA+TGG | - | Chr4:64854787-64854806 | MsG0480021825.01.T01:CDS | 45.0% |
| TTGCGTAGGATGGGATCCAT+TGG | + | Chr4:64854806-64854825 | None:intergenic | 50.0% | |
| CCAAAGCGAGAACACTGGGA+AGG | - | Chr4:64854772-64854791 | MsG0480021825.01.T01:CDS | 55.0% | |
| CCTTCCCAGTGTTCTCGCTT+TGG | + | Chr4:64854775-64854794 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 64854760 | 64854963 | 64854760 | ID=MsG0480021825.01;Name=MsG0480021825.01 |
| Chr4 | mRNA | 64854760 | 64854963 | 64854760 | ID=MsG0480021825.01.T01;Parent=MsG0480021825.01;Name=MsG0480021825.01.T01;_AED=0.50;_eAED=0.51;_QI=0|-1|0|1|-1|1|1|0|67 |
| Chr4 | exon | 64854760 | 64854963 | 64854760 | ID=MsG0480021825.01.T01:exon:20528;Parent=MsG0480021825.01.T01 |
| Chr4 | CDS | 64854760 | 64854963 | 64854760 | ID=MsG0480021825.01.T01:cds;Parent=MsG0480021825.01.T01 |
| Gene Sequence |
| Protein sequence |
