AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011401.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011401.01.T01 MTR_2g103750 79.268 164 5 1 1 164 1 135 2.66e-79 231
MsG0280011401.01.T01 MTR_4g010530 40.244 164 56 5 1 163 1 123 1.58e-23 90.1
MsG0280011401.01.T01 MTR_4g114600 33.714 175 71 8 1 162 1 143 6.16e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011401.01.T01 AT1G52565 35.882 170 52 5 3 164 6 126 1.63e-13 64.3
MsG0280011401.01.T01 AT3G15760 32.778 180 57 8 1 164 1 132 5.45e-11 57.8

Find 23 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCCATCATTTCAAATTCT+TGG 0.191389 2:+83479099 MsG0280011401.01.T01:CDS
GAGCGCAAAATTTGTGTGTT+AGG 0.249902 2:-83479141 None:intergenic
TACAAAGCCATTCTATTTGC+AGG 0.298881 2:-83479720 None:intergenic
TTGCGCTCTTGTTTATATTC+AGG 0.300388 2:+83479156 MsG0280011401.01.T01:CDS
CTTCCAAGAATTTGAAATGA+TGG 0.348228 2:-83479102 None:intergenic
TGATGGGTTAATCTTGTGTT+TGG 0.357672 2:-83479085 None:intergenic
ATAGAATGGCTTTGTATAAC+GGG 0.410953 2:+83479727 MsG0280011401.01.T01:CDS
GTTGTTCTTGACAAAGTGTA+AGG 0.413473 2:-83479475 None:intergenic
AGCGCAAAATTTGTGTGTTA+GGG 0.441560 2:-83479140 None:intergenic
AATAGAATGGCTTTGTATAA+CGG 0.457196 2:+83479726 MsG0280011401.01.T01:CDS
TTCCAAGAATTTGAAATGAT+GGG 0.463098 2:-83479101 None:intergenic
TTGAGTTGACTTGACTAGAA+AGG 0.495722 2:-83478956 None:intergenic
AAAATACACACAATATAGTA+AGG 0.497693 2:-83479513 None:intergenic
ACAAATGACTTCTCTCTCAC+AGG 0.497730 2:+83479005 MsG0280011401.01.T01:exon
TAGATTTGAAGAAAGTGTGA+AGG 0.513088 2:+83479596 MsG0280011401.01.T01:CDS
ATAGTAAGGACTAAGAACTA+AGG 0.525231 2:-83479499 None:intergenic
TGGAATTCCTGCAAATAGAA+TGG 0.533064 2:+83479713 MsG0280011401.01.T01:CDS
TTTCTCAGAGGAAAAGAAGA+GGG 0.546313 2:+83479536 MsG0280011401.01.T01:CDS
GTTAATTAGATATATAGATG+TGG 0.561719 2:-83478926 None:intergenic
TTAATTAGATATATAGATGT+GGG 0.565743 2:-83478925 None:intergenic
CAAATGACTTCTCTCTCACA+GGG 0.592780 2:+83479006 MsG0280011401.01.T01:exon
AGATATATAGATGTGGGTGT+AGG 0.594837 2:-83478919 None:intergenic
AGTGAAACAACGAAAACTCG+TGG 0.634965 2:+83479693 MsG0280011401.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCGATATTGATATTAATTA+AGG - Chr2:83479420-83479439 None:intergenic 15.0%
!! TTAATTAGATATATAGATGT+GGG - Chr2:83478928-83478947 None:intergenic 15.0%
!! AAAATACACACAATATAGTA+AGG - Chr2:83479516-83479535 None:intergenic 20.0%
!! AATATCAATATCGATATTGT+CGG + Chr2:83479425-83479444 MsG0280011401.01.T01:intron 20.0%
!! ATATCGATATTGATATTGTT+AGG - Chr2:83479325-83479344 None:intergenic 20.0%
!! GTTAATTAGATATATAGATG+TGG - Chr2:83478929-83478948 None:intergenic 20.0%
!! TCTACATACAAAATAAAGTA+AGG - Chr2:83479209-83479228 None:intergenic 20.0%
!!! TTACTTTATTTTGTATGTAG+AGG + Chr2:83479208-83479227 MsG0280011401.01.T01:intron 20.0%
!! TCATTTGTTGAAGAAAAAGA+AGG - Chr2:83478993-83479012 None:intergenic 25.0%
!! TTCCAAGAATTTGAAATGAT+GGG - Chr2:83479104-83479123 None:intergenic 25.0%
!!! AATAGAATGGCTTTGTATAA+CGG + Chr2:83479726-83479745 MsG0280011401.01.T01:CDS 25.0%
!!! TTTTTTATGACCTAACTCAA+CGG - Chr2:83479366-83479385 None:intergenic 25.0%
AACCCATCATTTCAAATTCT+TGG + Chr2:83479099-83479118 MsG0280011401.01.T01:CDS 30.0%
ATAGTAAGGACTAAGAACTA+AGG - Chr2:83479502-83479521 None:intergenic 30.0%
TAGCAAAAAAAGAGAAACAG+AGG + Chr2:83479640-83479659 MsG0280011401.01.T01:CDS 30.0%
! CTTCCAAGAATTTGAAATGA+TGG - Chr2:83479105-83479124 None:intergenic 30.0%
! TAGATTTGAAGAAAGTGTGA+AGG + Chr2:83479596-83479615 MsG0280011401.01.T01:CDS 30.0%
!! ATAGAATGGCTTTGTATAAC+GGG + Chr2:83479727-83479746 MsG0280011401.01.T01:CDS 30.0%
!! TTTGTATAACGGGATTTTGA+GGG + Chr2:83479737-83479756 MsG0280011401.01.T01:CDS 30.0%
!!! TGTGTGTATTTTTTTCTCAG+AGG + Chr2:83479524-83479543 MsG0280011401.01.T01:CDS 30.0%
AGCGCAAAATTTGTGTGTTA+GGG - Chr2:83479143-83479162 None:intergenic 35.0%
AGGTTAATCAAAAGAAGAGC+TGG - Chr2:83479189-83479208 None:intergenic 35.0%
GGAAAAAGCTAGATAAAGCA+TGG - Chr2:83479067-83479086 None:intergenic 35.0%
GTTGTTCTTGACAAAGTGTA+AGG - Chr2:83479396-83479415 None:intergenic 35.0%
TACAAAGCCATTCTATTTGC+AGG - Chr2:83479723-83479742 None:intergenic 35.0%
TGGAATTCCTGCAAATAGAA+TGG + Chr2:83479713-83479732 MsG0280011401.01.T01:CDS 35.0%
TTTCTCAGAGGAAAAGAAGA+GGG + Chr2:83479536-83479555 MsG0280011401.01.T01:CDS 35.0%
! TTGCGCTCTTGTTTATATTC+AGG + Chr2:83479156-83479175 MsG0280011401.01.T01:CDS 35.0%
! TTTTCTCAGAGGAAAAGAAG+AGG + Chr2:83479535-83479554 MsG0280011401.01.T01:CDS 35.0%
!! CTTTGTATAACGGGATTTTG+AGG + Chr2:83479736-83479755 MsG0280011401.01.T01:CDS 35.0%
!! TGATGGGTTAATCTTGTGTT+TGG - Chr2:83479088-83479107 None:intergenic 35.0%
!! TTGAGTTGACTTGACTAGAA+AGG - Chr2:83478959-83478978 None:intergenic 35.0%
ACAAATGACTTCTCTCTCAC+AGG + Chr2:83479005-83479024 MsG0280011401.01.T01:exon 40.0%
AGTGAAACAACGAAAACTCG+TGG + Chr2:83479693-83479712 MsG0280011401.01.T01:CDS 40.0%
CAAATGACTTCTCTCTCACA+GGG + Chr2:83479006-83479025 MsG0280011401.01.T01:exon 40.0%
GAGCGCAAAATTTGTGTGTT+AGG - Chr2:83479144-83479163 None:intergenic 40.0%
TACTGAAACTCACACGCATA+AGG + Chr2:83479242-83479261 MsG0280011401.01.T01:intron 40.0%
TCGATATTGTCCGTTGAGTT+AGG + Chr2:83479353-83479372 MsG0280011401.01.T01:intron 40.0%
! TCGATATTGTCGGTTAAGCT+AGG + Chr2:83479435-83479454 MsG0280011401.01.T01:intron 40.0%
ACACACAACTGTGTGGTACA+GGG + Chr2:83479274-83479293 MsG0280011401.01.T01:intron 45.0%
!! TTTTGAGGGATCGTGTGCAT+AGG + Chr2:83479751-83479770 MsG0280011401.01.T01:CDS 45.0%
CACACACAACTGTGTGGTAC+AGG + Chr2:83479273-83479292 MsG0280011401.01.T01:intron 50.0%
CGAACTCACACACAACTGTG+TGG + Chr2:83479267-83479286 MsG0280011401.01.T01:intron 50.0%
TGTGGTACAGGGTTCGAACT+CGG + Chr2:83479285-83479304 MsG0280011401.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 83478922 83479782 83478922 ID=MsG0280011401.01;Name=MsG0280011401.01
Chr2 mRNA 83478922 83479782 83478922 ID=MsG0280011401.01.T01;Parent=MsG0280011401.01;Name=MsG0280011401.01.T01;_AED=0.50;_eAED=0.56;_QI=87|0|0.5|1|0|0|2|0|164
Chr2 exon 83478922 83479177 83478922 ID=MsG0280011401.01.T01:exon:12749;Parent=MsG0280011401.01.T01
Chr2 exon 83479457 83479782 83479457 ID=MsG0280011401.01.T01:exon:12750;Parent=MsG0280011401.01.T01
Chr2 five_prime_UTR 83478922 83479008 83478922 ID=MsG0280011401.01.T01:five_prime_utr;Parent=MsG0280011401.01.T01
Chr2 CDS 83479009 83479177 83479009 ID=MsG0280011401.01.T01:cds;Parent=MsG0280011401.01.T01
Chr2 CDS 83479457 83479782 83479457 ID=MsG0280011401.01.T01:cds;Parent=MsG0280011401.01.T01
Gene Sequence

>MsG0280011401.01.T01

ATGACTTCTCTCTCACAGGGTCTTGTTTTCACTACAGCTATGCTTGTATCAAGCACCATGCTTTATCTAGCTTTTTCCAAACACAAGATTAACCCATCATTTCAAATTCTTGGAAGTCACGTTTCTCATGACCCTAACACACAAATTTTGCGCTCTTGTTTATATTCAGGTCATATAAATTTTTTAACCTTACACTTTGTCAAGAACAACTCCTTAGTTCTTAGTCCTTACTATATTGTGTGTATTTTTTTCTCAGAGGAAAAGAAGAGGGAGAGAAAGATGAATACAAAGAAAAATAAAAAGAAAGTTAGATTTGAAGAAAGTGTGAAGGAAGTAAGAGAAAAGAGTGAGATAGCAAAAAAAGAGAAACAGAGGAAACGAAACAGAGTACATAGCAACTGCAGAAGTGAAACAACGAAAACTCGTGGAATTCCTGCAAATAGAATGGCTTTGTATAACGGGATTTTGAGGGATCGTGTGCATAGGATTGAGTGA

Protein sequence

>MsG0280011401.01.T01

MTSLSQGLVFTTAMLVSSTMLYLAFSKHKINPSFQILGSHVSHDPNTQILRSCLYSGHINFLTLHFVKNNSLVLSPYYIVCIFFSEEKKRERKMNTKKNKKKVRFEESVKEVREKSEIAKKEKQRKRNRVHSNCRSETTKTRGIPANRMALYNGILRDRVHRIE*