AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021983.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021983.01.T01 MTR_4g080100 95.495 222 8 1 1 222 1 220 2.49e-144 412
MsG0480021983.01.T01 MTR_2g015690 53.191 141 64 1 34 172 24 164 1.35e-49 169
MsG0480021983.01.T01 MTR_4g087810 46.584 161 83 2 32 189 15 175 1.34e-43 153
MsG0480021983.01.T01 MTR_4g087830 44.375 160 86 2 33 189 7 166 5.74e-41 146
MsG0480021983.01.T01 MTR_4g077070 43.972 141 75 3 32 171 102 239 7.23e-34 129
MsG0480021983.01.T01 MTR_7g096140 39.716 141 84 1 31 171 75 214 2.99e-32 124
MsG0480021983.01.T01 MTR_7g096120 39.716 141 84 1 31 171 75 214 1.08e-31 122
MsG0480021983.01.T01 MTR_7g006400 41.304 138 68 3 34 171 109 233 8.84e-24 100
MsG0480021983.01.T01 MTR_7g081620 34.826 201 109 5 24 222 63 243 9.29e-24 100
MsG0480021983.01.T01 MTR_7g081645 33.862 189 91 7 24 203 70 233 5.72e-21 92.0
MsG0480021983.01.T01 MTR_5g013230 31.818 154 95 4 19 170 124 269 3.12e-18 84.7
MsG0480021983.01.T01 MTR_4g099510 34.532 139 81 4 34 170 133 263 1.76e-16 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021983.01.T01 AT2G42690 69.281 153 47 0 33 185 7 159 3.51e-74 233
MsG0480021983.01.T01 AT4G18550 46.528 144 74 2 34 174 40 183 1.01e-40 143
MsG0480021983.01.T01 AT4G18550 46.528 144 74 2 34 174 23 166 3.83e-40 144
MsG0480021983.01.T01 AT4G18550 46.528 144 74 2 34 174 40 183 5.14e-40 144
MsG0480021983.01.T01 AT1G06250 42.000 150 85 1 34 181 9 158 3.91e-34 128
MsG0480021983.01.T01 AT1G30370 40.127 157 89 3 15 171 102 253 1.73e-33 128
MsG0480021983.01.T01 AT1G51440 42.958 142 79 2 31 171 88 228 4.97e-33 126
MsG0480021983.01.T01 AT2G30550 43.056 144 75 4 31 171 102 241 1.29e-32 125
MsG0480021983.01.T01 AT2G30550 43.056 144 75 4 31 171 102 241 1.50e-32 125
MsG0480021983.01.T01 AT1G06800 41.379 145 77 5 31 171 86 226 3.47e-29 115
MsG0480021983.01.T01 AT1G06800 41.379 145 77 5 31 171 86 226 4.21e-29 115
MsG0480021983.01.T01 AT2G31690 34.483 145 90 1 33 177 91 230 5.39e-23 98.2
MsG0480021983.01.T01 AT1G05800 35.135 148 88 2 24 171 74 213 2.87e-22 96.3
MsG0480021983.01.T01 AT2G44810 36.429 140 82 4 34 171 90 224 9.63e-20 88.6
MsG0480021983.01.T01 AT4G16820 37.324 142 75 4 34 174 131 259 1.29e-19 88.6
MsG0480021983.01.T01 AT2G44810 37.037 135 78 4 39 171 5 134 6.19e-19 85.9

Find 81 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTTTGTTCCATGTTTCT+TGG 0.233840 4:+66802921 None:intergenic
TACCCTTATTATTGTTGTTT+GGG 0.238162 4:+66802426 None:intergenic
CCTCCCAAACAACAATAATA+AGG 0.244272 4:-66802429 MsG0480021983.01.T01:CDS
AACTCCTAGGCAACAACAAC+TGG 0.269686 4:-66802904 MsG0480021983.01.T01:CDS
ACCAACCCTTCATTACTTTA+GGG 0.280032 4:+66802157 None:intergenic
ATGTGGGTTGCGACGAATAA+AGG 0.282982 4:-66801582 MsG0480021983.01.T01:CDS
GTACCCTTATTATTGTTGTT+TGG 0.286185 4:+66802425 None:intergenic
AACCAACCCTTCATTACTTT+AGG 0.305402 4:+66802156 None:intergenic
GCTGCTCCTGAATCTGCTTC+TGG 0.315313 4:-66802485 MsG0480021983.01.T01:CDS
TGTGTCTGTTGCCATGTCTA+AGG 0.317714 4:+66802985 None:intergenic
CAGCAGCAGGTCCTCCTTCT+CGG 0.348405 4:+66801522 None:intergenic
GTTTCTTGGTTTATTGTTGT+TGG 0.361744 4:+66802935 None:intergenic
GTTGGGACCGTGAGTCTAAC+TGG 0.369388 4:-66802622 MsG0480021983.01.T01:CDS
GAGGTCTAGTGGGTTTAGTA+AGG 0.379673 4:+66802877 None:intergenic
AACCGCAGTAGATAGAGTTT+TGG 0.382882 4:+66802786 None:intergenic
GCGTAGGGAGGATGGGGAAT+GGG 0.409917 4:-66801553 MsG0480021983.01.T01:CDS
CATGACTTTGTTAAAGAATG+AGG 0.410558 4:+66802745 None:intergenic
TGGTTTATTGTTGTTGGTTG+TGG 0.412592 4:+66802941 None:intergenic
GTATGGTGCGTAGGGAGGAT+GGG 0.417927 4:-66801560 MsG0480021983.01.T01:CDS
GGTATGGTGCGTAGGGAGGA+TGG 0.422785 4:-66801561 MsG0480021983.01.T01:CDS
TTTCCCAGTTGTTGTTGCCT+AGG 0.428181 4:+66802900 None:intergenic
TAAGTCTTGTGATTACTTGA+AGG 0.431881 4:-66801627 MsG0480021983.01.T01:CDS
AGGTGAAGGTGAAGAGGAGT+TGG 0.435755 4:-66801660 MsG0480021983.01.T01:intron
TACTGCGGTTCAAGCCGTTA+TGG 0.436707 4:-66802773 MsG0480021983.01.T01:CDS
CTCCCAAACAACAATAATAA+GGG 0.439311 4:-66802428 MsG0480021983.01.T01:intron
AACAAACCAGAAGCAGATTC+AGG 0.440065 4:+66802479 None:intergenic
GAGTGAAGAATAAAAGCTTC+AGG 0.445100 4:+66802659 None:intergenic
CTCACTATCACGTGAGAGTT+GGG 0.447265 4:-66802639 MsG0480021983.01.T01:CDS
CAGAGTCCCTAAAGTAATGA+AGG 0.450917 4:-66802163 MsG0480021983.01.T01:CDS
GGATGAGGTTGCGGAGGTTG+AGG 0.468903 4:+66802858 None:intergenic
CGAATAAAGGTATGGTGCGT+AGG 0.476201 4:-66801569 MsG0480021983.01.T01:CDS
TGTCACGTGCCGGCGTCATG+TGG 0.478593 4:-66801599 MsG0480021983.01.T01:CDS
TGCGTAGGGAGGATGGGGAA+TGG 0.488524 4:-66801554 MsG0480021983.01.T01:CDS
CTCACTCACTCACTTTAACA+TGG 0.488693 4:-66803029 MsG0480021983.01.T01:exon
CCTTATTATTGTTGTTTGGG+AGG 0.489521 4:+66802429 None:intergenic
TTGTGTGGCGTGGTACTACT+AGG 0.495241 4:-66802535 MsG0480021983.01.T01:CDS
AGTTTGAGGTGAAGGTGAAG+AGG 0.496715 4:-66801666 MsG0480021983.01.T01:intron
CTGGAGTGGATTAACGTGTT+TGG 0.501745 4:-66802509 MsG0480021983.01.T01:CDS
TATGGTGCGTAGGGAGGATG+GGG 0.510574 4:-66801559 MsG0480021983.01.T01:CDS
GTGTCTGTTGCCATGTCTAA+GGG 0.516582 4:+66802986 None:intergenic
CGATGAACGGAGTAGAGAGT+TGG 0.517745 4:-66802576 MsG0480021983.01.T01:CDS
TTGCGGAGGTTGAGGTCTAG+TGG 0.521697 4:+66802866 None:intergenic
ACTCACTATCACGTGAGAGT+TGG 0.522634 4:-66802640 MsG0480021983.01.T01:CDS
CAACTCTCTACTCCGTTCAT+CGG 0.527868 4:+66802577 None:intergenic
CACAACGGAGGATGAGGTTG+CGG 0.530460 4:+66802849 None:intergenic
CAATACTCTTCAGCAGCAGC+AGG 0.532100 4:+66801509 None:intergenic
GACCGTGAGTCTAACTGGAT+TGG 0.532608 4:-66802617 MsG0480021983.01.T01:CDS
TGCGGAGGTTGAGGTCTAGT+GGG 0.534596 4:+66802867 None:intergenic
TTTATTGTTGTTGGTTGTGG+TGG 0.538715 4:+66802944 None:intergenic
ACACAACAGTGTAGTGTGTA+GGG 0.546335 4:+66802714 None:intergenic
GATGAACGGAGTAGAGAGTT+GGG 0.549457 4:-66802575 MsG0480021983.01.T01:CDS
GAATAAAGGTATGGTGCGTA+GGG 0.553192 4:-66801568 MsG0480021983.01.T01:CDS
CGCAACCTCATCCTCCGTTG+TGG 0.560178 4:-66802848 MsG0480021983.01.T01:CDS
GTCACGTGCCGGCGTCATGT+GGG 0.564995 4:-66801598 MsG0480021983.01.T01:CDS
AGTCACCACAACGGAGGATG+AGG 0.574935 4:+66802843 None:intergenic
GAAACATGGAACAAACTCCT+AGG 0.575157 4:-66802917 MsG0480021983.01.T01:CDS
TCACTCACTCACTTTAACAT+GGG 0.576884 4:-66803028 MsG0480021983.01.T01:exon
TCGCTGTTAGTTCCGATGAA+CGG 0.581063 4:-66802589 MsG0480021983.01.T01:CDS
GTCGTTGTTGAAGGAATCGT+AGG 0.582102 4:+66802808 None:intergenic
CAACAATAAACCAAGAAACA+TGG 0.587322 4:-66802931 MsG0480021983.01.T01:CDS
GAAGGAAGAGTGTCACGTGC+CGG 0.589437 4:-66801609 MsG0480021983.01.T01:CDS
GACACAACAGTGTAGTGTGT+AGG 0.592811 4:+66802713 None:intergenic
AATGGGTTGATGCACCGAGA+AGG 0.593988 4:-66801536 MsG0480021983.01.T01:CDS
GGGTTGATGCACCGAGAAGG+AGG 0.598990 4:-66801533 MsG0480021983.01.T01:CDS
TAAAGGTATGGTGCGTAGGG+AGG 0.609458 4:-66801565 MsG0480021983.01.T01:CDS
TAGCAGCAGCAGTGACGATG+AGG 0.609517 4:-66802193 MsG0480021983.01.T01:CDS
GCGTGGTACTACTAGGGACC+TGG 0.616633 4:-66802528 MsG0480021983.01.T01:CDS
AACGGAGGATGAGGTTGCGG+AGG 0.617333 4:+66802852 None:intergenic
ACTCCTAGGCAACAACAACT+GGG 0.618469 4:-66802903 MsG0480021983.01.T01:CDS
AGAGTCCCTAAAGTAATGAA+GGG 0.619750 4:-66802162 MsG0480021983.01.T01:intron
CAAACACGTTAATCCACTCC+AGG 0.633191 4:+66802510 None:intergenic
CGTCGCAACCCACATGACGC+CGG 0.646702 4:+66801590 None:intergenic
GTCGTGAAATATATGTTGTG+TGG 0.660451 4:-66802550 MsG0480021983.01.T01:CDS
AACCAATCCAGTTAGACTCA+CGG 0.662669 4:+66802615 None:intergenic
GGTTGCGACGAATAAAGGTA+TGG 0.662928 4:-66801577 MsG0480021983.01.T01:CDS
GAAATATATGTTGTGTGGCG+TGG 0.672854 4:-66802545 MsG0480021983.01.T01:CDS
GTACTACTAGGGACCTGGAG+TGG 0.677827 4:-66802523 MsG0480021983.01.T01:CDS
TGTGTGGCGTGGTACTACTA+GGG 0.683087 4:-66802534 MsG0480021983.01.T01:CDS
TTTGTATGAAGTCACCACAA+CGG 0.704168 4:+66802834 None:intergenic
GACCAAAACTCTATCTACTG+CGG 0.719453 4:-66802788 MsG0480021983.01.T01:CDS
GTATGAAGTCACCACAACGG+AGG 0.734924 4:+66802837 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTAAAAGTTTCAAAATTGA+AGG - Chr4:66802657-66802676 MsG0480021983.01.T01:CDS 20.0%
!! TAGAAACATGACATTTATAT+AGG - Chr4:66802204-66802223 MsG0480021983.01.T01:intron 20.0%
! AAATCTTATACAAATTCACC+CGG + Chr4:66802185-66802204 None:intergenic 25.0%
!! AACTTTTAACCTATCATTGA+AGG + Chr4:66802647-66802666 None:intergenic 25.0%
!! GTTTTTGTAAAGGTGATTTT+GGG + Chr4:66802428-66802447 None:intergenic 25.0%
!!! TACCCTTATTATTGTTGTTT+GGG + Chr4:66802114-66802133 None:intergenic 25.0%
!!! TGTTTTTGTAAAGGTGATTT+TGG + Chr4:66802429-66802448 None:intergenic 25.0%
ACATTATCCAGGAAGTTATT+GGG - Chr4:66802714-66802733 MsG0480021983.01.T01:CDS 30.0%
ATTATCCTTAACTCCATATC+CGG - Chr4:66802163-66802182 MsG0480021983.01.T01:CDS 30.0%
CAACAATAAACCAAGAAACA+TGG - Chr4:66801606-66801625 MsG0480021983.01.T01:CDS 30.0%
CATGACTTTGTTAAAGAATG+AGG + Chr4:66801795-66801814 None:intergenic 30.0%
CTCCCAAACAACAATAATAA+GGG - Chr4:66802109-66802128 MsG0480021983.01.T01:intron 30.0%
GTTTCTTGGTTTATTGTTGT+TGG + Chr4:66801605-66801624 None:intergenic 30.0%
! AATTGAAGGTTTTACATGTG+AGG - Chr4:66802671-66802690 MsG0480021983.01.T01:CDS 30.0%
! GTACCCTTATTATTGTTGTT+TGG + Chr4:66802115-66802134 None:intergenic 30.0%
! TAAGTCTTGTGATTACTTGA+AGG - Chr4:66802910-66802929 MsG0480021983.01.T01:CDS 30.0%
AAATCCAAGTGTGGTATTAG+TGG - Chr4:66802507-66802526 MsG0480021983.01.T01:CDS 35.0%
AACCAACCCTTCATTACTTT+AGG + Chr4:66802384-66802403 None:intergenic 35.0%
AATCCAAGTGTGGTATTAGT+GGG - Chr4:66802508-66802527 MsG0480021983.01.T01:CDS 35.0%
ACCAACCCTTCATTACTTTA+GGG + Chr4:66802383-66802402 None:intergenic 35.0%
AGAGTCCCTAAAGTAATGAA+GGG - Chr4:66802375-66802394 MsG0480021983.01.T01:intron 35.0%
CAAAAAAACTCCCTTAGACA+TGG - Chr4:66801541-66801560 MsG0480021983.01.T01:CDS 35.0%
CAAGAATGAAAATCCAAGTG+TGG - Chr4:66802498-66802517 MsG0480021983.01.T01:CDS 35.0%
CAATAACTTCCTGGATAATG+TGG + Chr4:66802715-66802734 None:intergenic 35.0%
CACATTATCCAGGAAGTTAT+TGG - Chr4:66802713-66802732 MsG0480021983.01.T01:CDS 35.0%
CATTATCCAGGAAGTTATTG+GGG - Chr4:66802715-66802734 MsG0480021983.01.T01:CDS 35.0%
CCTCCCAAACAACAATAATA+AGG - Chr4:66802108-66802127 MsG0480021983.01.T01:intron 35.0%
GAGTGAAGAATAAAAGCTTC+AGG + Chr4:66801881-66801900 None:intergenic 35.0%
GATCTTATACCACATTATCC+AGG - Chr4:66802703-66802722 MsG0480021983.01.T01:CDS 35.0%
GTCGTGAAATATATGTTGTG+TGG - Chr4:66801987-66802006 MsG0480021983.01.T01:intron 35.0%
TATACAAATTCACCCGGATA+TGG + Chr4:66802179-66802198 None:intergenic 35.0%
TCAATGTGAGTGCTTTGATT+TGG - Chr4:66802553-66802572 MsG0480021983.01.T01:CDS 35.0%
TCACTCACTCACTTTAACAT+GGG - Chr4:66801509-66801528 MsG0480021983.01.T01:CDS 35.0%
TTATCCTTAACTCCATATCC+GGG - Chr4:66802164-66802183 MsG0480021983.01.T01:CDS 35.0%
TTATTGGGGTAGAGTATACA+GGG - Chr4:66802729-66802748 MsG0480021983.01.T01:CDS 35.0%
TTTGTATGAAGTCACCACAA+CGG + Chr4:66801706-66801725 None:intergenic 35.0%
! AGAATGAGGTTTTGCCATAA+CGG + Chr4:66801781-66801800 None:intergenic 35.0%
! TGGTTTATTGTTGTTGGTTG+TGG + Chr4:66801599-66801618 None:intergenic 35.0%
! TTTATTGTTGTTGGTTGTGG+TGG + Chr4:66801596-66801615 None:intergenic 35.0%
!! CCTTATTATTGTTGTTTGGG+AGG + Chr4:66802111-66802130 None:intergenic 35.0%
!!! TCTCACGCTTGTTTTTGTAA+AGG + Chr4:66802438-66802457 None:intergenic 35.0%
AAAAACAAGCGTGAGAACAC+AGG - Chr4:66802442-66802461 MsG0480021983.01.T01:CDS 40.0%
AACAAACCAGAAGCAGATTC+AGG + Chr4:66802061-66802080 None:intergenic 40.0%
AACCAATCCAGTTAGACTCA+CGG + Chr4:66801925-66801944 None:intergenic 40.0%
ACACAACAGTGTAGTGTGTA+GGG + Chr4:66801826-66801845 None:intergenic 40.0%
CAGAGTCCCTAAAGTAATGA+AGG - Chr4:66802374-66802393 MsG0480021983.01.T01:intron 40.0%
CTCACTCACTCACTTTAACA+TGG - Chr4:66801508-66801527 MsG0480021983.01.T01:CDS 40.0%
GAAACATGGAACAAACTCCT+AGG - Chr4:66801620-66801639 MsG0480021983.01.T01:CDS 40.0%
GAAATATATGTTGTGTGGCG+TGG - Chr4:66801992-66802011 MsG0480021983.01.T01:intron 40.0%
GACCAAAACTCTATCTACTG+CGG - Chr4:66801749-66801768 MsG0480021983.01.T01:intron 40.0%
GATTCGAAGAATCGAGTGAT+TGG - Chr4:66802793-66802812 MsG0480021983.01.T01:CDS 40.0%
GCAAGCTATGTTGCATATTG+TGG - Chr4:66802824-66802843 MsG0480021983.01.T01:CDS 40.0%
GGAACAAAGCCTTCAATGAT+AGG - Chr4:66802635-66802654 MsG0480021983.01.T01:CDS 40.0%
GTATTAGTGGGACATAGTCT+AGG - Chr4:66802520-66802539 MsG0480021983.01.T01:CDS 40.0%
GTTATTGGGGTAGAGTATAC+AGG - Chr4:66802728-66802747 MsG0480021983.01.T01:CDS 40.0%
TCCCTAAAGTAATGAAGGGT+TGG - Chr4:66802379-66802398 MsG0480021983.01.T01:intron 40.0%
! AACCGCAGTAGATAGAGTTT+TGG + Chr4:66801754-66801773 None:intergenic 40.0%
! ATATTGTGGCTGGTTGGAAT+GGG - Chr4:66802838-66802857 MsG0480021983.01.T01:CDS 40.0%
! GAATAAAGGTATGGTGCGTA+GGG - Chr4:66802969-66802988 MsG0480021983.01.T01:CDS 40.0%
! GGAGTTTGTTCCATGTTTCT+TGG + Chr4:66801619-66801638 None:intergenic 40.0%
!! AGAGTTTTGGTCGTTGTTGA+AGG + Chr4:66801741-66801760 None:intergenic 40.0%
!! ATGTGAGTGCTTTGATTTGG+TGG - Chr4:66802556-66802575 MsG0480021983.01.T01:CDS 40.0%
!! TGCTTTGATTTGGTGGAGAA+TGG - Chr4:66802563-66802582 MsG0480021983.01.T01:CDS 40.0%
AACTCCTAGGCAACAACAAC+TGG - Chr4:66801633-66801652 MsG0480021983.01.T01:CDS 45.0%
ACTCACTATCACGTGAGAGT+TGG - Chr4:66801897-66801916 MsG0480021983.01.T01:intron 45.0%
ACTCCTAGGCAACAACAACT+GGG - Chr4:66801634-66801653 MsG0480021983.01.T01:CDS 45.0%
ACTCTACCCCAATAACTTCC+TGG + Chr4:66802724-66802743 None:intergenic 45.0%
CAAACACGTTAATCCACTCC+AGG + Chr4:66802030-66802049 None:intergenic 45.0%
CAACTCTCTACTCCGTTCAT+CGG + Chr4:66801963-66801982 None:intergenic 45.0%
CCAAACACAAATGCAGCAAC+GGG + Chr4:66802602-66802621 None:intergenic 45.0%
CCCAAACACAAATGCAGCAA+CGG + Chr4:66802603-66802622 None:intergenic 45.0%
CCGTTGCTGCATTTGTGTTT+GGG - Chr4:66802600-66802619 MsG0480021983.01.T01:CDS 45.0%
CTCACTATCACGTGAGAGTT+GGG - Chr4:66801898-66801917 MsG0480021983.01.T01:intron 45.0%
GACACAACAGTGTAGTGTGT+AGG + Chr4:66801827-66801846 None:intergenic 45.0%
GATGAACGGAGTAGAGAGTT+GGG - Chr4:66801962-66801981 MsG0480021983.01.T01:intron 45.0%
GCTATGTTGCATATTGTGGC+TGG - Chr4:66802828-66802847 MsG0480021983.01.T01:CDS 45.0%
GGTTGCGACGAATAAAGGTA+TGG - Chr4:66802960-66802979 MsG0480021983.01.T01:CDS 45.0%
GTCGTTGTTGAAGGAATCGT+AGG + Chr4:66801732-66801751 None:intergenic 45.0%
GTGTCTGTTGCCATGTCTAA+GGG + Chr4:66801554-66801573 None:intergenic 45.0%
TAGAGTATACAGGGACAGAG+TGG - Chr4:66802738-66802757 MsG0480021983.01.T01:CDS 45.0%
TCGCTGTTAGTTCCGATGAA+CGG - Chr4:66801948-66801967 MsG0480021983.01.T01:intron 45.0%
TGTCCCACTAATACCACACT+TGG + Chr4:66802514-66802533 None:intergenic 45.0%
TGTGTCTGTTGCCATGTCTA+AGG + Chr4:66801555-66801574 None:intergenic 45.0%
TGTTGCATATTGTGGCTGGT+TGG - Chr4:66802832-66802851 MsG0480021983.01.T01:CDS 45.0%
TTCACCCGGATATGGAGTTA+AGG + Chr4:66802171-66802190 None:intergenic 45.0%
! AGTTTGAGGTGAAGGTGAAG+AGG - Chr4:66802871-66802890 MsG0480021983.01.T01:CDS 45.0%
! ATGTGGGTTGCGACGAATAA+AGG - Chr4:66802955-66802974 MsG0480021983.01.T01:CDS 45.0%
! CATATTGTGGCTGGTTGGAA+TGG - Chr4:66802837-66802856 MsG0480021983.01.T01:CDS 45.0%
! CGAATAAAGGTATGGTGCGT+AGG - Chr4:66802968-66802987 MsG0480021983.01.T01:CDS 45.0%
! TTTCCCAGTTGTTGTTGCCT+AGG + Chr4:66801640-66801659 None:intergenic 45.0%
!! AAGGCTTTGTTCCCAACTTG+GGG + Chr4:66802628-66802647 None:intergenic 45.0%
!! CTGGAGTGGATTAACGTGTT+TGG - Chr4:66802028-66802047 MsG0480021983.01.T01:intron 45.0%
!! GAAGGCTTTGTTCCCAACTT+GGG + Chr4:66802629-66802648 None:intergenic 45.0%
!! GAGGTCTAGTGGGTTTAGTA+AGG + Chr4:66801663-66801682 None:intergenic 45.0%
!! TGAAGGCTTTGTTCCCAACT+TGG + Chr4:66802630-66802649 None:intergenic 45.0%
AGGTGAAGGTGAAGAGGAGT+TGG - Chr4:66802877-66802896 MsG0480021983.01.T01:CDS 50.0%
CAATACTCTTCAGCAGCAGC+AGG + Chr4:66803031-66803050 None:intergenic 50.0%
CCCGTTGCTGCATTTGTGTT+TGG - Chr4:66802599-66802618 MsG0480021983.01.T01:CDS 50.0%
CGATGAACGGAGTAGAGAGT+TGG - Chr4:66801961-66801980 MsG0480021983.01.T01:intron 50.0%
CTGGTTGGAATGGGAGTGAT+GGG - Chr4:66802847-66802866 MsG0480021983.01.T01:CDS 50.0%
GACCGTGAGTCTAACTGGAT+TGG - Chr4:66801920-66801939 MsG0480021983.01.T01:intron 50.0%
GTATGAAGTCACCACAACGG+AGG + Chr4:66801703-66801722 None:intergenic 50.0%
TACTGCGGTTCAAGCCGTTA+TGG - Chr4:66801764-66801783 MsG0480021983.01.T01:intron 50.0%
TGGTTGGAATGGGAGTGATG+GGG - Chr4:66802848-66802867 MsG0480021983.01.T01:CDS 50.0%
TGTGTGGCGTGGTACTACTA+GGG - Chr4:66802003-66802022 MsG0480021983.01.T01:intron 50.0%
TTGTGTGGCGTGGTACTACT+AGG - Chr4:66802002-66802021 MsG0480021983.01.T01:intron 50.0%
! TAAAGGTATGGTGCGTAGGG+AGG - Chr4:66802972-66802991 MsG0480021983.01.T01:CDS 50.0%
! TGATGGGGAGTTTGAGGTGA+AGG - Chr4:66802863-66802882 MsG0480021983.01.T01:CDS 50.0%
!! AATGGGTTGATGCACCGAGA+AGG - Chr4:66803001-66803020 MsG0480021983.01.T01:CDS 50.0%
!! AGGCTTTGTTCCCAACTTGG+GGG + Chr4:66802627-66802646 None:intergenic 50.0%
!! AGGTGATTTTGGGTCGTCTG+AGG + Chr4:66802418-66802437 None:intergenic 50.0%
AGTCACCACAACGGAGGATG+AGG + Chr4:66801697-66801716 None:intergenic 55.0%
CACAACGGAGGATGAGGTTG+CGG + Chr4:66801691-66801710 None:intergenic 55.0%
GCTGCTCCTGAATCTGCTTC+TGG - Chr4:66802052-66802071 MsG0480021983.01.T01:intron 55.0%
GCTGGTTGGAATGGGAGTGA+TGG - Chr4:66802846-66802865 MsG0480021983.01.T01:CDS 55.0%
GTACTACTAGGGACCTGGAG+TGG - Chr4:66802014-66802033 MsG0480021983.01.T01:intron 55.0%
GTATGGTGCGTAGGGAGGAT+GGG - Chr4:66802977-66802996 MsG0480021983.01.T01:CDS 55.0%
GTGTTTGGGTCCCCCAAGTT+GGG - Chr4:66802614-66802633 MsG0480021983.01.T01:CDS 55.0%
GTTGGGACCGTGAGTCTAAC+TGG - Chr4:66801915-66801934 MsG0480021983.01.T01:intron 55.0%
TAGCAGCAGCAGTGACGATG+AGG - Chr4:66802344-66802363 MsG0480021983.01.T01:intron 55.0%
TATGGTGCGTAGGGAGGATG+GGG - Chr4:66802978-66802997 MsG0480021983.01.T01:CDS 55.0%
TGCGGAGGTTGAGGTCTAGT+GGG + Chr4:66801673-66801692 None:intergenic 55.0%
TGTGTTTGGGTCCCCCAAGT+TGG - Chr4:66802613-66802632 MsG0480021983.01.T01:CDS 55.0%
TTGCGGAGGTTGAGGTCTAG+TGG + Chr4:66801674-66801693 None:intergenic 55.0%
! TGGGAGTGATGGGGAGTTTG+AGG - Chr4:66802857-66802876 MsG0480021983.01.T01:CDS 55.0%
!! GAAGGAAGAGTGTCACGTGC+CGG - Chr4:66802928-66802947 MsG0480021983.01.T01:CDS 55.0%
AACGGAGGATGAGGTTGCGG+AGG + Chr4:66801688-66801707 None:intergenic 60.0%
CAGCAGCAGGTCCTCCTTCT+CGG + Chr4:66803018-66803037 None:intergenic 60.0%
CGCAACCTCATCCTCCGTTG+TGG - Chr4:66801689-66801708 MsG0480021983.01.T01:intron 60.0%
GCGTAGGGAGGATGGGGAAT+GGG - Chr4:66802984-66803003 MsG0480021983.01.T01:CDS 60.0%
GCGTGGTACTACTAGGGACC+TGG - Chr4:66802009-66802028 MsG0480021983.01.T01:intron 60.0%
GGATGAGGTTGCGGAGGTTG+AGG + Chr4:66801682-66801701 None:intergenic 60.0%
GGTATGGTGCGTAGGGAGGA+TGG - Chr4:66802976-66802995 MsG0480021983.01.T01:CDS 60.0%
TGCGTAGGGAGGATGGGGAA+TGG - Chr4:66802983-66803002 MsG0480021983.01.T01:CDS 60.0%
!! GGGTTGATGCACCGAGAAGG+AGG - Chr4:66803004-66803023 MsG0480021983.01.T01:CDS 60.0%
CGTCGCAACCCACATGACGC+CGG + Chr4:66802950-66802969 None:intergenic 65.0%
! GTCACGTGCCGGCGTCATGT+GGG - Chr4:66802939-66802958 MsG0480021983.01.T01:CDS 65.0%
! TGTCACGTGCCGGCGTCATG+TGG - Chr4:66802938-66802957 MsG0480021983.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 66801508 66803051 66801508 ID=MsG0480021983.01;Name=MsG0480021983.01
Chr4 mRNA 66801508 66803051 66801508 ID=MsG0480021983.01.T01;Parent=MsG0480021983.01;Name=MsG0480021983.01.T01;_AED=0.50;_eAED=0.50;_QI=19|0.5|0.33|1|1|1|3|0|276
Chr4 exon 66801508 66801674 66801508 ID=MsG0480021983.01.T01:exon:20962;Parent=MsG0480021983.01.T01
Chr4 exon 66802163 66802222 66802163 ID=MsG0480021983.01.T01:exon:20961;Parent=MsG0480021983.01.T01
Chr4 exon 66802429 66803051 66802429 ID=MsG0480021983.01.T01:exon:20960;Parent=MsG0480021983.01.T01
Chr4 five_prime_UTR 66803033 66803051 66803033 ID=MsG0480021983.01.T01:five_prime_utr;Parent=MsG0480021983.01.T01
Chr4 CDS 66802429 66803032 66802429 ID=MsG0480021983.01.T01:cds;Parent=MsG0480021983.01.T01
Chr4 CDS 66802163 66802222 66802163 ID=MsG0480021983.01.T01:cds;Parent=MsG0480021983.01.T01
Chr4 CDS 66801508 66801674 66801508 ID=MsG0480021983.01.T01:cds;Parent=MsG0480021983.01.T01
Gene Sequence

>MsG0480021983.01.T01

ATGGGAATAGCACACAAAAAAACTCCCTTAGACATGGCAACAGACACAGAAACATCATCAACAACACCACCACAACCAACAACAATAAACCAAGAAACATGGAACAAACTCCTAGGCAACAACAACTGGGAAACCTTACTAAACCCACTAGACCTCAACCTCCGCAACCTCATCCTCCGTTGTGGTGACTTCATACAAACAACCTACGATTCCTTCAACAACGACCAAAACTCTATCTACTGCGGTTCAAGCCGTTATGGCAAAACCTCATTCTTTAACAAAGTCATGCTTGAAAACCCTACACACTACACTGTTGTGTCTTTTCTTTATGCTACAGCACGTGTATCTGTTCCTGAAGCTTTTATTCTTCACTCACTATCACGTGAGAGTTGGGACCGTGAGTCTAACTGGATTGGTTACATCGCTGTTAGTTCCGATGAACGGAGTAGAGAGTTGGGTCGTCGTGAAATATATGTTGTGTGGCGTGGTACTACTAGGGACCTGGAGTGGATTAACGTGTTTGGTGCTGCTCCTGAATCTGCTTCTGGTTTGTTGAGTGCTAAAAGTCTTCGTGAATTCAACCTCCCAAACAACAATAATAAGGATGACAGTAGCAGCAGCAGTGACGATGAGGATGACAACAGAGTCCCTAAAGTAATGAAGGGTGAAGAGGAGTTGGCTTTAGTGAATAAGTCTTGTGATTACTTGAAGGAAGAGTGTCACGTGCCGGCGTCATGTGGGTTGCGACGAATAAAGGTATGGTGCGTAGGGAGGATGGGGAATGGGTTGATGCACCGAGAAGGAGGACCTGCTGCTGCTGAAGAGTATTGA
Protein sequence

>MsG0480021983.01.T01

MGIAHKKTPLDMATDTETSSTTPPQPTTINQETWNKLLGNNNWETLLNPLDLNLRNLILRCGDFIQTTYDSFNNDQNSIYCGSSRYGKTSFFNKVMLENPTHYTVVSFLYATARVSVPEAFILHSLSRESWDRESNWIGYIAVSSDERSRELGRREIYVVWRGTTRDLEWINVFGAAPESASGLLSAKSLREFNLPNNNNKDDSSSSSDDEDDNRVPKVMKGEEELALVNKSCDYLKEECHVPASCGLRRIKVWCVGRMGNGLMHREGGPAAAEEY*