AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027875.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027875.01.T01 MTR_5g464340 94.921 315 16 0 1 315 1 315 0.0 617
MsG0580027875.01.T01 MTR_5g064340 94.921 315 16 0 1 315 1 315 0.0 617
MsG0580027875.01.T01 MTR_5g064345 93.968 315 19 0 1 315 1 315 0.0 609
MsG0580027875.01.T01 MTR_5g064360 93.038 316 21 1 1 315 1 316 0.0 602
MsG0580027875.01.T01 MTR_3g074860 78.571 294 50 3 22 315 24 304 4.70e-170 474
MsG0580027875.01.T01 MTR_3g074890 78.231 294 51 4 22 315 24 304 2.10e-168 470
MsG0580027875.01.T01 MTR_3g074830 71.795 312 83 4 8 315 35 345 1.08e-153 434
MsG0580027875.01.T01 MTR_4g094240 61.053 285 97 3 31 315 30 300 4.30e-121 350
MsG0580027875.01.T01 MTR_2g009070 53.628 317 131 6 1 315 2 304 7.32e-120 347
MsG0580027875.01.T01 MTR_4g033790 36.082 291 164 8 21 300 50 329 2.40e-48 165
MsG0580027875.01.T01 MTR_1g076470 35.395 291 167 8 21 300 49 329 2.20e-45 157
MsG0580027875.01.T01 MTR_8g071310 69.118 68 21 0 248 315 18 85 1.13e-25 98.2
MsG0580027875.01.T01 MTR_4g013230 73.846 65 14 1 251 315 79 140 2.22e-24 96.7
MsG0580027875.01.T01 MTR_8g088900 73.846 65 17 0 251 315 308 372 3.36e-24 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027875.01.T01 AT4G08950 72.241 299 73 6 23 315 20 314 4.70e-148 419
MsG0580027875.01.T01 AT1G35140 64.013 314 107 2 1 314 1 308 4.10e-143 406
MsG0580027875.01.T01 AT5G64260 58.576 309 115 6 10 315 7 305 7.16e-122 352
MsG0580027875.01.T01 AT5G09440 58.759 274 84 6 44 315 32 278 8.15e-102 300
MsG0580027875.01.T01 AT5G51550 35.974 303 172 8 21 312 40 331 1.10e-48 166
MsG0580027875.01.T01 AT3G02970 31.438 299 169 7 46 313 37 330 3.30e-45 157
MsG0580027875.01.T01 AT2G35150 34.568 324 183 11 8 311 2 316 2.98e-44 154
MsG0580027875.01.T01 AT2G17230 32.680 306 184 9 19 312 62 357 5.55e-43 152

Find 63 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGACCCACATCGTTATT+TGG 0.213136 5:-70149260 None:intergenic
TCTTATGGTGCACGCGTAAA+TGG 0.225421 5:+70149119 MsG0580027875.01.T01:CDS
GGAACCCATGGTTCTTCTTA+TGG 0.283880 5:+70149104 MsG0580027875.01.T01:CDS
CTTGACGAGAATTACTCTTT+AGG 0.286620 5:+70148960 MsG0580027875.01.T01:CDS
TGGTTATGCTGGGGCCCTTT+TGG 0.286824 5:+70149424 MsG0580027875.01.T01:CDS
GCACTTTGTGGACCATAAAT+TGG 0.307975 5:-70149224 None:intergenic
GCTTCCAAAGGAGCTTCTTT+TGG 0.308698 5:-70149359 None:intergenic
TCCATACCAAATGATGTTTA+CGG 0.318337 5:-70148754 None:intergenic
GAAACAAGTTCGCGTACATT+TGG 0.347338 5:+70149147 MsG0580027875.01.T01:CDS
AAATTTCCGTAAACATCATT+TGG 0.360811 5:+70148748 MsG0580027875.01.T01:CDS
AGCTCCTTTGGAAGCTGCTT+CGG 0.362716 5:+70149367 MsG0580027875.01.T01:CDS
CCATAAATTGGTTGGTGAAA+AGG 0.364618 5:-70149212 None:intergenic
TTGACGCGAGTTTAACGATT+TGG 0.366058 5:-70149001 None:intergenic
AGGACCAAAAGAAGCTCCTT+TGG 0.376105 5:+70149355 MsG0580027875.01.T01:CDS
CGGTTAAAAGAGGGCCTTTG+TGG 0.388607 5:-70148722 None:intergenic
AATAACGATGTGGGTCTTGA+TGG 0.390121 5:+70149263 MsG0580027875.01.T01:CDS
GCAGCTGATGTGTTAGTTGA+TGG 0.407698 5:+70149062 MsG0580027875.01.T01:CDS
AAACAAGTTCGCGTACATTT+GGG 0.409022 5:+70149148 MsG0580027875.01.T01:CDS
GGAGCTTATCCTGGTTATGC+TGG 0.412712 5:+70149413 MsG0580027875.01.T01:CDS
ATCGTTAAACTCGCGTCAAA+AGG 0.419827 5:+70149005 MsG0580027875.01.T01:CDS
TACAACGCTAATGGTGATAA+TGG 0.420824 5:+70149470 MsG0580027875.01.T01:CDS
GATGGTTGTGATGTGGTTGT+TGG 0.425520 5:-70148843 None:intergenic
TCCGTAAACATCATTTGGTA+TGG 0.445565 5:+70148753 MsG0580027875.01.T01:CDS
CTAACACATCAGCTGCTGTT+AGG 0.449185 5:-70149055 None:intergenic
GGAACCGTAACCAATCCTTT+TGG 0.451430 5:+70149317 MsG0580027875.01.T01:CDS
TGAAAATATCCATTACCAAA+AGG 0.461381 5:-70149332 None:intergenic
TGCTGTTAGGACAACGTTGA+TGG 0.463697 5:-70149042 None:intergenic
GCAACCAATGGTGCACTTTG+TGG 0.466845 5:-70149236 None:intergenic
GAAGCTGCTTCGGCTTGTAC+CGG 0.475241 5:+70149377 MsG0580027875.01.T01:CDS
TGTACCGGTGTTTATGCTAA+AGG 0.481549 5:+70149392 MsG0580027875.01.T01:CDS
ATATCCATTACCAAAAGGAT+TGG 0.488324 5:-70149327 None:intergenic
TATGCTAAAGGAGCTTATCC+TGG 0.497752 5:+70149404 MsG0580027875.01.T01:CDS
GTTATGATTGCTCTTTGAGA+TGG 0.502947 5:-70148786 None:intergenic
ATTACCAAAAGGATTGGTTA+CGG 0.504727 5:-70149321 None:intergenic
GAGTACCTAGCGAGAGAGCT+AGG 0.504789 5:-70148932 None:intergenic
TGGTCCACAAAGTGCACCAT+TGG 0.507521 5:+70149232 MsG0580027875.01.T01:CDS
GGTGCTAGCTACAACGCTAA+TGG 0.508260 5:+70149461 MsG0580027875.01.T01:CDS
GAGCTTATCCTGGTTATGCT+GGG 0.517406 5:+70149414 MsG0580027875.01.T01:CDS
TCGTTATTTGGTGCAACCAA+TGG 0.521248 5:-70149248 None:intergenic
AATTCTGAAACTCAATGTGC+TGG 0.549654 5:+70149176 MsG0580027875.01.T01:CDS
CGATGTGGGTCTTGATGGAA+TGG 0.552866 5:+70149268 MsG0580027875.01.T01:CDS
AAGTTCGCGTACATTTGGGT+TGG 0.562836 5:+70149152 MsG0580027875.01.T01:CDS
TCAAGTAGGTGTGGAACCCA+TGG 0.564282 5:+70149092 MsG0580027875.01.T01:CDS
AAAAGGGCCCCAGCATAACC+AGG 0.566264 5:-70149422 None:intergenic
ACGCTAATGGTGATAATGGA+AGG 0.567526 5:+70149474 MsG0580027875.01.T01:CDS
GTTAACCTAGCTCTCTCGCT+AGG 0.568037 5:+70148927 MsG0580027875.01.T01:CDS
AACCTCAGTTTGTTCTACGT+TGG 0.574270 5:+70149529 MsG0580027875.01.T01:CDS
TTCCACCACGTGGCAACTGA+TGG 0.582258 5:-70148861 None:intergenic
AGCTTATCCTGGTTATGCTG+GGG 0.590478 5:+70149415 MsG0580027875.01.T01:CDS
AACCATCAGTTGCCACGTGG+TGG 0.610757 5:+70148859 MsG0580027875.01.T01:CDS
TTTGTGGACCATAAATTGGT+TGG 0.611426 5:-70149220 None:intergenic
CTTAAATTTCAATACCACAA+AGG 0.623830 5:+70148708 MsG0580027875.01.T01:CDS
GTGCACCATAAGAAGAACCA+TGG 0.625761 5:-70149109 None:intergenic
GGTTGCACCAAATAACGATG+TGG 0.628110 5:+70149253 MsG0580027875.01.T01:CDS
CAAGCCGAAGCAGCTTCCAA+AGG 0.645366 5:-70149371 None:intergenic
TACCAACGTAGAACAAACTG+AGG 0.649916 5:-70149531 None:intergenic
TTCGGTTGATTTCCACCACG+TGG 0.652662 5:-70148871 None:intergenic
TGCACCATAAGAAGAACCAT+GGG 0.655555 5:-70149108 None:intergenic
AATGTGCTGGTCAATGCGCG+TGG 0.660041 5:+70149189 MsG0580027875.01.T01:CDS
GTTGCACCAAATAACGATGT+GGG 0.661869 5:+70149254 MsG0580027875.01.T01:CDS
GGCAACTGATGGTTGTGATG+TGG 0.667076 5:-70148850 None:intergenic
AGCTCCTTTAGCATAAACAC+CGG 0.684494 5:-70149396 None:intergenic
CACAACCATCAGTTGCCACG+TGG 0.702852 5:+70148856 MsG0580027875.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATTTCCGTAAACATCATT+TGG + Chr5:70148748-70148767 MsG0580027875.01.T01:CDS 25.0%
! CTTAAATTTCAATACCACAA+AGG + Chr5:70148708-70148727 MsG0580027875.01.T01:CDS 25.0%
! TGAAAATATCCATTACCAAA+AGG - Chr5:70149335-70149354 None:intergenic 25.0%
!!! AAATTTTACCGGTTAAAAGA+GGG - Chr5:70148734-70148753 None:intergenic 25.0%
!!! AGTGAGTTGATAGTATTTTT+CGG - Chr5:70148892-70148911 None:intergenic 25.0%
!!! TTTGGTAATGGATATTTTCA+AGG + Chr5:70149335-70149354 MsG0580027875.01.T01:CDS 25.0%
ATATCCATTACCAAAAGGAT+TGG - Chr5:70149330-70149349 None:intergenic 30.0%
TCCATACCAAATGATGTTTA+CGG - Chr5:70148757-70148776 None:intergenic 30.0%
! GATGTTTACGGAAATTTTAC+CGG - Chr5:70148745-70148764 None:intergenic 30.0%
!! ATTACCAAAAGGATTGGTTA+CGG - Chr5:70149324-70149343 None:intergenic 30.0%
!! GAAATTTTACCGGTTAAAAG+AGG - Chr5:70148735-70148754 None:intergenic 30.0%
!!! TTGATGGATTTTGTTCAAGT+AGG + Chr5:70149078-70149097 MsG0580027875.01.T01:CDS 30.0%
AAACAAGTTCGCGTACATTT+GGG + Chr5:70149148-70149167 MsG0580027875.01.T01:CDS 35.0%
CTTGACGAGAATTACTCTTT+AGG + Chr5:70148960-70148979 MsG0580027875.01.T01:CDS 35.0%
TACAACGCTAATGGTGATAA+TGG + Chr5:70149470-70149489 MsG0580027875.01.T01:CDS 35.0%
TCCGTAAACATCATTTGGTA+TGG + Chr5:70148753-70148772 MsG0580027875.01.T01:CDS 35.0%
TTTGTGGACCATAAATTGGT+TGG - Chr5:70149223-70149242 None:intergenic 35.0%
! CCTTTTCACCAACCAATTTA+TGG + Chr5:70149212-70149231 MsG0580027875.01.T01:CDS 35.0%
! GTAACCAATCCTTTTGGTAA+TGG + Chr5:70149323-70149342 MsG0580027875.01.T01:CDS 35.0%
! GTAGAACAAACTGAGGTTTT+AGG - Chr5:70149527-70149546 None:intergenic 35.0%
! GTTATGATTGCTCTTTGAGA+TGG - Chr5:70148789-70148808 None:intergenic 35.0%
!! AATTCTGAAACTCAATGTGC+TGG + Chr5:70149176-70149195 MsG0580027875.01.T01:CDS 35.0%
!! CCATAAATTGGTTGGTGAAA+AGG - Chr5:70149215-70149234 None:intergenic 35.0%
!!! AATGTTGCTAGTCTTTTAGC+TGG + Chr5:70149296-70149315 MsG0580027875.01.T01:CDS 35.0%
!!! ACTTGTTTTGTCCACCAAAA+GGG - Chr5:70149441-70149460 None:intergenic 35.0%
!!! GATGTGGTTGTTGGTTTTTT+GGG - Chr5:70148837-70148856 None:intergenic 35.0%
!!! TGATGTGGTTGTTGGTTTTT+TGG - Chr5:70148838-70148857 None:intergenic 35.0%
!!! TTTTTTGGGAGAAGAAAGTG+AGG - Chr5:70148823-70148842 None:intergenic 35.0%
AATAACGATGTGGGTCTTGA+TGG + Chr5:70149263-70149282 MsG0580027875.01.T01:CDS 40.0%
AGCTCCTTTAGCATAAACAC+CGG - Chr5:70149399-70149418 None:intergenic 40.0%
ATCGTTAAACTCGCGTCAAA+AGG + Chr5:70149005-70149024 MsG0580027875.01.T01:CDS 40.0%
GAAACAAGTTCGCGTACATT+TGG + Chr5:70149147-70149166 MsG0580027875.01.T01:CDS 40.0%
GCACTTTGTGGACCATAAAT+TGG - Chr5:70149227-70149246 None:intergenic 40.0%
GTTGCACCAAATAACGATGT+GGG + Chr5:70149254-70149273 MsG0580027875.01.T01:CDS 40.0%
TACCAACGTAGAACAAACTG+AGG - Chr5:70149534-70149553 None:intergenic 40.0%
TATGCTAAAGGAGCTTATCC+TGG + Chr5:70149404-70149423 MsG0580027875.01.T01:CDS 40.0%
TCAAGACCCACATCGTTATT+TGG - Chr5:70149263-70149282 None:intergenic 40.0%
TGCACCATAAGAAGAACCAT+GGG - Chr5:70149111-70149130 None:intergenic 40.0%
TTGACGCGAGTTTAACGATT+TGG - Chr5:70149004-70149023 None:intergenic 40.0%
! AACCTCAGTTTGTTCTACGT+TGG + Chr5:70149529-70149548 MsG0580027875.01.T01:CDS 40.0%
! GGATTTTGTTCAAGTAGGTG+TGG + Chr5:70149083-70149102 MsG0580027875.01.T01:CDS 40.0%
! TGTACCGGTGTTTATGCTAA+AGG + Chr5:70149392-70149411 MsG0580027875.01.T01:CDS 40.0%
!! ACGCTAATGGTGATAATGGA+AGG + Chr5:70149474-70149493 MsG0580027875.01.T01:CDS 40.0%
!! CTTTTGGTGGACAAAACAAG+TGG + Chr5:70149440-70149459 MsG0580027875.01.T01:CDS 40.0%
!! TCGTTATTTGGTGCAACCAA+TGG - Chr5:70149251-70149270 None:intergenic 40.0%
!!! CACTTGTTTTGTCCACCAAA+AGG - Chr5:70149442-70149461 None:intergenic 40.0%
!!! GTTTTAGGATCAACAATCGC+AGG - Chr5:70149512-70149531 None:intergenic 40.0%
AAGTTCGCGTACATTTGGGT+TGG + Chr5:70149152-70149171 MsG0580027875.01.T01:CDS 45.0%
AGCTTATCCTGGTTATGCTG+GGG + Chr5:70149415-70149434 MsG0580027875.01.T01:CDS 45.0%
AGGACCAAAAGAAGCTCCTT+TGG + Chr5:70149355-70149374 MsG0580027875.01.T01:CDS 45.0%
CTAACACATCAGCTGCTGTT+AGG - Chr5:70149058-70149077 None:intergenic 45.0%
GAGCTTATCCTGGTTATGCT+GGG + Chr5:70149414-70149433 MsG0580027875.01.T01:CDS 45.0%
GATGGTTGTGATGTGGTTGT+TGG - Chr5:70148846-70148865 None:intergenic 45.0%
GCAGCTGATGTGTTAGTTGA+TGG + Chr5:70149062-70149081 MsG0580027875.01.T01:CDS 45.0%
GGAACCCATGGTTCTTCTTA+TGG + Chr5:70149104-70149123 MsG0580027875.01.T01:CDS 45.0%
GGTTGCACCAAATAACGATG+TGG + Chr5:70149253-70149272 MsG0580027875.01.T01:CDS 45.0%
GTGCACCATAAGAAGAACCA+TGG - Chr5:70149112-70149131 None:intergenic 45.0%
TCTTATGGTGCACGCGTAAA+TGG + Chr5:70149119-70149138 MsG0580027875.01.T01:CDS 45.0%
TGCTGTTAGGACAACGTTGA+TGG - Chr5:70149045-70149064 None:intergenic 45.0%
! CACAAAGGCCCTCTTTTAAC+CGG + Chr5:70148723-70148742 MsG0580027875.01.T01:CDS 45.0%
! GCTTCCAAAGGAGCTTCTTT+TGG - Chr5:70149362-70149381 None:intergenic 45.0%
! GGAACCGTAACCAATCCTTT+TGG + Chr5:70149317-70149336 MsG0580027875.01.T01:CDS 45.0%
CGATGTGGGTCTTGATGGAA+TGG + Chr5:70149268-70149287 MsG0580027875.01.T01:CDS 50.0%
CGGTTAAAAGAGGGCCTTTG+TGG - Chr5:70148725-70148744 None:intergenic 50.0%
GCAACCAATGGTGCACTTTG+TGG - Chr5:70149239-70149258 None:intergenic 50.0%
GGAGCTTATCCTGGTTATGC+TGG + Chr5:70149413-70149432 MsG0580027875.01.T01:CDS 50.0%
GGCAACTGATGGTTGTGATG+TGG - Chr5:70148853-70148872 None:intergenic 50.0%
GGTGCTAGCTACAACGCTAA+TGG + Chr5:70149461-70149480 MsG0580027875.01.T01:CDS 50.0%
TCAAGTAGGTGTGGAACCCA+TGG + Chr5:70149092-70149111 MsG0580027875.01.T01:CDS 50.0%
TGGTCCACAAAGTGCACCAT+TGG + Chr5:70149232-70149251 MsG0580027875.01.T01:CDS 50.0%
! AGCTCCTTTGGAAGCTGCTT+CGG + Chr5:70149367-70149386 MsG0580027875.01.T01:CDS 50.0%
! GTTAACCTAGCTCTCTCGCT+AGG + Chr5:70148927-70148946 MsG0580027875.01.T01:CDS 50.0%
! TTCGGTTGATTTCCACCACG+TGG - Chr5:70148874-70148893 None:intergenic 50.0%
AAAAGGGCCCCAGCATAACC+AGG - Chr5:70149425-70149444 None:intergenic 55.0%
AACCATCAGTTGCCACGTGG+TGG + Chr5:70148859-70148878 MsG0580027875.01.T01:CDS 55.0%
CAAGCCGAAGCAGCTTCCAA+AGG - Chr5:70149374-70149393 None:intergenic 55.0%
CACAACCATCAGTTGCCACG+TGG + Chr5:70148856-70148875 MsG0580027875.01.T01:CDS 55.0%
GAGTACCTAGCGAGAGAGCT+AGG - Chr5:70148935-70148954 None:intergenic 55.0%
TTCCACCACGTGGCAACTGA+TGG - Chr5:70148864-70148883 None:intergenic 55.0%
! AATGTGCTGGTCAATGCGCG+TGG + Chr5:70149189-70149208 MsG0580027875.01.T01:CDS 55.0%
! GAAGCTGCTTCGGCTTGTAC+CGG + Chr5:70149377-70149396 MsG0580027875.01.T01:CDS 55.0%
! TGGTTATGCTGGGGCCCTTT+TGG + Chr5:70149424-70149443 MsG0580027875.01.T01:CDS 55.0%
! TTATGCTGGGGCCCTTTTGG+TGG + Chr5:70149427-70149446 MsG0580027875.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 70148609 70149556 70148609 ID=MsG0580027875.01;Name=MsG0580027875.01
Chr5 mRNA 70148609 70149556 70148609 ID=MsG0580027875.01.T01;Parent=MsG0580027875.01;Name=MsG0580027875.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|315
Chr5 exon 70148609 70149556 70148609 ID=MsG0580027875.01.T01:exon:25520;Parent=MsG0580027875.01.T01
Chr5 CDS 70148609 70149556 70148609 ID=MsG0580027875.01.T01:cds;Parent=MsG0580027875.01.T01
Gene Sequence

>MsG0580027875.01.T01

ATGGCATCTTTTAACAATAGTGCAATCTTCATTCTTTTTCTCACAATTGCAATAGTTCAATTGAGTTCAGCTAGAAAACTCACTGAATCAGATCAACAACTTAAATTTCAATACCACAAAGGCCCTCTTTTAACCGGTAAAATTTCCGTAAACATCATTTGGTATGGAAAATTCAAACCATCTCAAAGAGCAATCATAACTGATTTCATTACCTCACTTTCTTCTCCCAAAAAACCAACAACCACATCACAACCATCAGTTGCCACGTGGTGGAAATCAACCGAAAAATACTATCAACTCACTAGCAACAAGAAATCAGTTAACCTAGCTCTCTCGCTAGGTACTCAAATTCTTGACGAGAATTACTCTTTAGGAAAATCTCTCACAACGAACCAAATCGTTAAACTCGCGTCAAAAGGACAACTACAAAACGCCATCAACGTTGTCCTAACAGCAGCTGATGTGTTAGTTGATGGATTTTGTTCAAGTAGGTGTGGAACCCATGGTTCTTCTTATGGTGCACGCGTAAATGGAAAGAGAAACAAGTTCGCGTACATTTGGGTTGGAAATTCTGAAACTCAATGTGCTGGTCAATGCGCGTGGCCTTTTCACCAACCAATTTATGGTCCACAAAGTGCACCATTGGTTGCACCAAATAACGATGTGGGTCTTGATGGAATGGTTATAAATGTTGCTAGTCTTTTAGCTGGAACCGTAACCAATCCTTTTGGTAATGGATATTTTCAAGGACCAAAAGAAGCTCCTTTGGAAGCTGCTTCGGCTTGTACCGGTGTTTATGCTAAAGGAGCTTATCCTGGTTATGCTGGGGCCCTTTTGGTGGACAAAACAAGTGGTGCTAGCTACAACGCTAATGGTGATAATGGAAGGAAGTATTTGCTGCCTGCGATTGTTGATCCTAAAACCTCAGTTTGTTCTACGTTGGTATAA

Protein sequence

>MsG0580027875.01.T01

MASFNNSAIFILFLTIAIVQLSSARKLTESDQQLKFQYHKGPLLTGKISVNIIWYGKFKPSQRAIITDFITSLSSPKKPTTTSQPSVATWWKSTEKYYQLTSNKKSVNLALSLGTQILDENYSLGKSLTTNQIVKLASKGQLQNAINVVLTAADVLVDGFCSSRCGTHGSSYGARVNGKRNKFAYIWVGNSETQCAGQCAWPFHQPIYGPQSAPLVAPNNDVGLDGMVINVASLLAGTVTNPFGNGYFQGPKEAPLEAASACTGVYAKGAYPGYAGALLVDKTSGASYNANGDNGRKYLLPAIVDPKTSVCSTLV*