Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022723.01.T01 | XP_013457271.1 | 95.732 | 164 | 7 | 0 | 2 | 165 | 64 | 227 | 2.95E-114 | 339 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022723.01.T01 | G8A1D8 | 95.732 | 164 | 7 | 0 | 2 | 165 | 64 | 227 | 1.41e-114 | 339 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003744.01 | MsG0480022723.01 | 0.806520 | 7.905805e-50 | 4.300025e-47 |
| MsG0180004252.01 | MsG0480022723.01 | 0.830599 | 2.681679e-55 | 2.838723e-52 |
| MsG0180006050.01 | MsG0480022723.01 | 0.808338 | 3.252401e-50 | 1.854842e-47 |
| MsG0480020144.01 | MsG0480022723.01 | 0.845816 | 3.207234e-59 | 5.411169e-56 |
| MsG0480022723.01 | MsG0580024311.01 | 0.849791 | 2.577558e-60 | 4.942669e-57 |
| MsG0480022723.01 | MsG0580024409.01 | 0.810885 | 9.225951e-51 | 5.628438e-48 |
| MsG0480022723.01 | MsG0580024956.01 | 0.842177 | 3.031297e-58 | 4.558233e-55 |
| MsG0480022723.01 | MsG0780040742.01 | 0.810403 | 1.173108e-50 | 7.064347e-48 |
| MsG0480022723.01 | MsG0880047464.01 | 0.817884 | 2.616137e-52 | 1.928619e-49 |
| MsG0480022723.01 | MsG0880047745.01 | 0.811588 | 6.495525e-51 | 4.036543e-48 |
| MsG0280008764.01 | MsG0480022723.01 | 0.800662 | 1.297393e-48 | 6.075502e-46 |
| MsG0280010287.01 | MsG0480022723.01 | 0.817840 | 2.676789e-52 | 1.970995e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022723.01.T01 | MTR_4g094420 | 95.732 | 164 | 7 | 0 | 2 | 165 | 64 | 227 | 3.57e-118 | 339 |
| MsG0480022723.01.T01 | MTR_3g073240 | 58.537 | 164 | 65 | 1 | 2 | 165 | 61 | 221 | 1.59e-64 | 200 |
| MsG0480022723.01.T01 | MTR_7g059110 | 41.000 | 200 | 82 | 4 | 2 | 165 | 37 | 236 | 6.21e-46 | 154 |
| MsG0480022723.01.T01 | MTR_4g113730 | 37.629 | 194 | 85 | 3 | 2 | 159 | 61 | 254 | 5.65e-39 | 135 |
| MsG0480022723.01.T01 | MTR_8g085870 | 40.000 | 175 | 90 | 3 | 2 | 166 | 59 | 228 | 1.07e-37 | 134 |
| MsG0480022723.01.T01 | MTR_1g009790 | 41.463 | 164 | 87 | 2 | 2 | 165 | 39 | 193 | 6.22e-36 | 127 |
| MsG0480022723.01.T01 | MTR_1g009790 | 41.463 | 164 | 87 | 2 | 2 | 165 | 39 | 193 | 1.08e-35 | 126 |
| MsG0480022723.01.T01 | MTR_7g059110 | 45.652 | 138 | 70 | 2 | 28 | 165 | 141 | 273 | 5.73e-35 | 126 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022723.01.T01 | AT1G68140 | 57.055 | 163 | 52 | 2 | 2 | 164 | 66 | 210 | 6.89e-64 | 199 |
| MsG0480022723.01.T01 | AT1G68140 | 57.055 | 163 | 52 | 2 | 2 | 164 | 66 | 210 | 9.58e-64 | 199 |
| MsG0480022723.01.T01 | AT1G68140 | 57.055 | 163 | 52 | 2 | 2 | 164 | 66 | 210 | 9.58e-64 | 199 |
| MsG0480022723.01.T01 | AT1G68140 | 57.055 | 163 | 52 | 2 | 2 | 164 | 66 | 210 | 9.58e-64 | 199 |
| MsG0480022723.01.T01 | AT4G08460 | 54.878 | 164 | 49 | 2 | 2 | 165 | 60 | 198 | 2.50e-55 | 176 |
| MsG0480022723.01.T01 | AT4G08460 | 54.878 | 164 | 49 | 2 | 2 | 165 | 60 | 198 | 2.50e-55 | 176 |
| MsG0480022723.01.T01 | AT4G08460 | 54.878 | 164 | 49 | 2 | 2 | 165 | 60 | 198 | 2.50e-55 | 176 |
| MsG0480022723.01.T01 | AT4G08460 | 54.878 | 164 | 49 | 2 | 2 | 165 | 60 | 198 | 2.50e-55 | 176 |
| MsG0480022723.01.T01 | AT1G77770 | 52.439 | 164 | 51 | 2 | 2 | 165 | 42 | 178 | 3.33e-49 | 160 |
| MsG0480022723.01.T01 | AT1G77770 | 52.439 | 164 | 51 | 2 | 2 | 165 | 42 | 178 | 3.69e-49 | 159 |
| MsG0480022723.01.T01 | AT1G77770 | 52.439 | 164 | 51 | 2 | 2 | 165 | 42 | 178 | 3.69e-49 | 159 |
| MsG0480022723.01.T01 | AT3G24740 | 38.974 | 195 | 88 | 2 | 2 | 165 | 37 | 231 | 1.87e-45 | 152 |
| MsG0480022723.01.T01 | AT3G24740 | 38.974 | 195 | 88 | 2 | 2 | 165 | 37 | 231 | 1.87e-45 | 152 |
| MsG0480022723.01.T01 | AT3G24740 | 38.974 | 195 | 88 | 2 | 2 | 165 | 37 | 231 | 1.87e-45 | 152 |
| MsG0480022723.01.T01 | AT3G24740 | 38.974 | 195 | 88 | 2 | 2 | 165 | 37 | 231 | 1.87e-45 | 152 |
| MsG0480022723.01.T01 | AT3G24740 | 38.974 | 195 | 88 | 2 | 2 | 165 | 37 | 231 | 1.87e-45 | 152 |
| MsG0480022723.01.T01 | AT3G24740 | 38.974 | 195 | 88 | 2 | 2 | 165 | 37 | 231 | 1.87e-45 | 152 |
| MsG0480022723.01.T01 | AT4G31410 | 37.805 | 164 | 87 | 1 | 2 | 165 | 45 | 193 | 6.12e-33 | 119 |
| MsG0480022723.01.T01 | AT4G31410 | 37.805 | 164 | 87 | 1 | 2 | 165 | 45 | 193 | 6.12e-33 | 119 |
| MsG0480022723.01.T01 | AT4G31410 | 37.805 | 164 | 87 | 1 | 2 | 165 | 45 | 193 | 6.12e-33 | 119 |
| MsG0480022723.01.T01 | AT3G25910 | 44.898 | 98 | 54 | 0 | 64 | 161 | 166 | 263 | 3.52e-25 | 99.8 |
| MsG0480022723.01.T01 | AT1G80220 | 34.375 | 160 | 75 | 3 | 2 | 160 | 58 | 188 | 1.36e-24 | 96.3 |
| MsG0480022723.01.T01 | AT1G15430 | 33.333 | 153 | 75 | 1 | 2 | 154 | 64 | 189 | 6.42e-23 | 92.0 |
| MsG0480022723.01.T01 | AT1G15430 | 33.333 | 153 | 75 | 1 | 2 | 154 | 64 | 189 | 6.42e-23 | 92.0 |
| MsG0480022723.01.T01 | AT2G26050 | 32.877 | 146 | 70 | 2 | 11 | 155 | 38 | 156 | 2.12e-22 | 89.7 |
Find 45 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATGTAGGGGTCAGGTTAA+AGG | 0.313947 | 4:+75969041 | MsG0480022723.01.T01:CDS |
| CTCGTTTGTTGGTAACTATA+AGG | 0.382098 | 4:+75969133 | MsG0480022723.01.T01:CDS |
| CTCCATTTCTGCTCATGATC+AGG | 0.382612 | 4:-75969212 | None:intergenic |
| CAGGATAACTGCTCGTTTGT+TGG | 0.399950 | 4:+75969122 | MsG0480022723.01.T01:CDS |
| ATCCTGATCATGAGCAGAAA+TGG | 0.401144 | 4:+75969210 | MsG0480022723.01.T01:CDS |
| AAGGCTGGTTCCACACATAT+AGG | 0.406115 | 4:-75968859 | None:intergenic |
| CACAGTGACATCAGCCATAC+CGG | 0.416376 | 4:+75969274 | MsG0480022723.01.T01:CDS |
| AGGCTGGTTCCACACATATA+GGG | 0.438782 | 4:-75968858 | None:intergenic |
| CTTGTACTGATCAAGGCAGT+TGG | 0.469418 | 4:-75968889 | None:intergenic |
| AAGCTTTCTTGTACTGATCA+AGG | 0.474158 | 4:-75968896 | None:intergenic |
| CTTTAACCTGACCCCTACAT+AGG | 0.477911 | 4:-75969040 | None:intergenic |
| TCAGCACGTAATGGACAGCC+TGG | 0.482846 | 4:+75968933 | MsG0480022723.01.T01:CDS |
| AGAAATGGAGATGGCTCGAG+TGG | 0.483380 | 4:+75969225 | MsG0480022723.01.T01:CDS |
| TGATCAGGATCTACCGTCCT+TGG | 0.489674 | 4:-75969197 | None:intergenic |
| TTCGAATCATGGAACATGAA+TGG | 0.498200 | 4:-75968975 | None:intergenic |
| TTTAACCTGACCCCTACATA+GGG | 0.513903 | 4:-75969039 | None:intergenic |
| ATATGTGTGGAACCAGCCTT+CGG | 0.515412 | 4:+75968862 | MsG0480022723.01.T01:CDS |
| ACCGTCCTTGGACGTGCTGA+AGG | 0.515499 | 4:-75969185 | None:intergenic |
| CATGAGGTGAGTTCGAATCA+TGG | 0.515586 | 4:-75968986 | None:intergenic |
| ATCTCCAAAACCACTGCCCC+CGG | 0.521448 | 4:-75969293 | None:intergenic |
| ACAGTGACATCAGCCATACC+GGG | 0.525370 | 4:+75969275 | MsG0480022723.01.T01:CDS |
| AAAGTAGTTTCAGCACGTAA+TGG | 0.533445 | 4:+75968924 | MsG0480022723.01.T01:CDS |
| GTAACTTCATTCTTCTCATG+AGG | 0.537744 | 4:-75969002 | None:intergenic |
| GCAGTTGGAATGCCGAAGGC+TGG | 0.539273 | 4:-75968874 | None:intergenic |
| TCGTTGAGAAAGTCTCGCAC+AGG | 0.558517 | 4:-75969080 | None:intergenic |
| AAAGAAAAGAAGTTGCATGC+AGG | 0.567458 | 4:+75969103 | MsG0480022723.01.T01:CDS |
| TTATCAATAGAACCGTCCCC+AGG | 0.573058 | 4:-75968951 | None:intergenic |
| AGCATCCTTCAGCACGTCCA+AGG | 0.573539 | 4:+75969180 | MsG0480022723.01.T01:CDS |
| TCCTTCAGCACGTCCAAGGA+CGG | 0.580285 | 4:+75969184 | MsG0480022723.01.T01:CDS |
| GTAGGGGTCAGGTTAAAGGT+TGG | 0.581654 | 4:+75969045 | MsG0480022723.01.T01:CDS |
| GGTTAAAGGTTGGACTGTCG+TGG | 0.586406 | 4:+75969055 | MsG0480022723.01.T01:CDS |
| CAGCACGTAATGGACAGCCT+GGG | 0.586526 | 4:+75968934 | MsG0480022723.01.T01:CDS |
| GGAGTTGAAGAAGCATGTGC+GGG | 0.591242 | 4:+75969154 | MsG0480022723.01.T01:CDS |
| CAAGGCAGTTGGAATGCCGA+AGG | 0.614907 | 4:-75968878 | None:intergenic |
| CGTAATGGACAGCCTGGGGA+CGG | 0.618439 | 4:+75968939 | MsG0480022723.01.T01:CDS |
| ATCATGAGCAGAAATGGAGA+TGG | 0.625471 | 4:+75969216 | MsG0480022723.01.T01:CDS |
| GGTTGCCGTCCCTATATGTG+TGG | 0.629919 | 4:+75968849 | MsG0480022723.01.T01:CDS |
| AGGAGTTGAAGAAGCATGTG+CGG | 0.633946 | 4:+75969153 | MsG0480022723.01.T01:CDS |
| TAACCTGACCCCTACATAGG+GGG | 0.655585 | 4:-75969037 | None:intergenic |
| GAAATGGAGATGGCTCGAGT+GGG | 0.657077 | 4:+75969226 | MsG0480022723.01.T01:CDS |
| CAGTGACATCAGCCATACCG+GGG | 0.657376 | 4:+75969276 | MsG0480022723.01.T01:CDS |
| TGGTTCCACACATATAGGGA+CGG | 0.664592 | 4:-75968854 | None:intergenic |
| AGCACGTAATGGACAGCCTG+GGG | 0.675144 | 4:+75968935 | MsG0480022723.01.T01:CDS |
| TTAACCTGACCCCTACATAG+GGG | 0.708225 | 4:-75969038 | None:intergenic |
| AGTGACATCAGCCATACCGG+GGG | 0.710151 | 4:+75969277 | MsG0480022723.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTTGTTCTTCTCATGACAA+GGG | + | Chr4:75968828-75968847 | MsG0480022723.01.T01:CDS | 30.0% |
| AAAGAAAAGAAGTTGCATGC+AGG | + | Chr4:75969103-75969122 | MsG0480022723.01.T01:CDS | 35.0% | |
| AAAGTAGTTTCAGCACGTAA+TGG | + | Chr4:75968924-75968943 | MsG0480022723.01.T01:CDS | 35.0% | |
| GTAACTTCATTCTTCTCATG+AGG | - | Chr4:75969005-75969024 | None:intergenic | 35.0% | |
| TTCGAATCATGGAACATGAA+TGG | - | Chr4:75968978-75968997 | None:intergenic | 35.0% | |
| ! | AAGCTTTCTTGTACTGATCA+AGG | - | Chr4:75968899-75968918 | None:intergenic | 35.0% |
| ! | CTTTTGTTCTTCTCATGACA+AGG | + | Chr4:75968827-75968846 | MsG0480022723.01.T01:CDS | 35.0% |
| !! | CTCGTTTGTTGGTAACTATA+AGG | + | Chr4:75969133-75969152 | MsG0480022723.01.T01:CDS | 35.0% |
| ATCATGAGCAGAAATGGAGA+TGG | + | Chr4:75969216-75969235 | MsG0480022723.01.T01:CDS | 40.0% | |
| ATCCTGATCATGAGCAGAAA+TGG | + | Chr4:75969210-75969229 | MsG0480022723.01.T01:CDS | 40.0% | |
| TTTAACCTGACCCCTACATA+GGG | - | Chr4:75969042-75969061 | None:intergenic | 40.0% | |
| ATATGTGTGGAACCAGCCTT+CGG | + | Chr4:75968862-75968881 | MsG0480022723.01.T01:CDS | 45.0% | |
| CAGGATAACTGCTCGTTTGT+TGG | + | Chr4:75969122-75969141 | MsG0480022723.01.T01:CDS | 45.0% | |
| CATGAGGTGAGTTCGAATCA+TGG | - | Chr4:75968989-75969008 | None:intergenic | 45.0% | |
| CTATGTAGGGGTCAGGTTAA+AGG | + | Chr4:75969041-75969060 | MsG0480022723.01.T01:CDS | 45.0% | |
| CTCCATTTCTGCTCATGATC+AGG | - | Chr4:75969215-75969234 | None:intergenic | 45.0% | |
| CTTTAACCTGACCCCTACAT+AGG | - | Chr4:75969043-75969062 | None:intergenic | 45.0% | |
| TGGTTCCACACATATAGGGA+CGG | - | Chr4:75968857-75968876 | None:intergenic | 45.0% | |
| TTAACCTGACCCCTACATAG+GGG | - | Chr4:75969041-75969060 | None:intergenic | 45.0% | |
| TTATCAATAGAACCGTCCCC+AGG | - | Chr4:75968954-75968973 | None:intergenic | 45.0% | |
| ! | AAGGCTGGTTCCACACATAT+AGG | - | Chr4:75968862-75968881 | None:intergenic | 45.0% |
| ! | AGGAGTTGAAGAAGCATGTG+CGG | + | Chr4:75969153-75969172 | MsG0480022723.01.T01:CDS | 45.0% |
| ! | AGGCTGGTTCCACACATATA+GGG | - | Chr4:75968861-75968880 | None:intergenic | 45.0% |
| ! | CTTGTACTGATCAAGGCAGT+TGG | - | Chr4:75968892-75968911 | None:intergenic | 45.0% |
| ACAGTGACATCAGCCATACC+GGG | + | Chr4:75969275-75969294 | MsG0480022723.01.T01:CDS | 50.0% | |
| ACATAGGGGGCAAGCAAGTT+CGG | - | Chr4:75969027-75969046 | None:intergenic | 50.0% | |
| ACTTGCTTGCCCCCTATGTA+GGG | + | Chr4:75969028-75969047 | MsG0480022723.01.T01:CDS | 50.0% | |
| AGAAATGGAGATGGCTCGAG+TGG | + | Chr4:75969225-75969244 | MsG0480022723.01.T01:CDS | 50.0% | |
| CACAGTGACATCAGCCATAC+CGG | + | Chr4:75969274-75969293 | MsG0480022723.01.T01:CDS | 50.0% | |
| GAAATGGAGATGGCTCGAGT+GGG | + | Chr4:75969226-75969245 | MsG0480022723.01.T01:CDS | 50.0% | |
| GGTTAAAGGTTGGACTGTCG+TGG | + | Chr4:75969055-75969074 | MsG0480022723.01.T01:CDS | 50.0% | |
| GTAGGGGTCAGGTTAAAGGT+TGG | + | Chr4:75969045-75969064 | MsG0480022723.01.T01:CDS | 50.0% | |
| TAACCTGACCCCTACATAGG+GGG | - | Chr4:75969040-75969059 | None:intergenic | 50.0% | |
| TCGTTGAGAAAGTCTCGCAC+AGG | - | Chr4:75969083-75969102 | None:intergenic | 50.0% | |
| TGATCAGGATCTACCGTCCT+TGG | - | Chr4:75969200-75969219 | None:intergenic | 50.0% | |
| ! | AACTTGCTTGCCCCCTATGT+AGG | + | Chr4:75969027-75969046 | MsG0480022723.01.T01:CDS | 50.0% |
| ! | GGAGTTGAAGAAGCATGTGC+GGG | + | Chr4:75969154-75969173 | MsG0480022723.01.T01:CDS | 50.0% |
| AGCATCCTTCAGCACGTCCA+AGG | + | Chr4:75969180-75969199 | MsG0480022723.01.T01:CDS | 55.0% | |
| AGTGACATCAGCCATACCGG+GGG | + | Chr4:75969277-75969296 | MsG0480022723.01.T01:CDS | 55.0% | |
| ATCTCCAAAACCACTGCCCC+CGG | - | Chr4:75969296-75969315 | None:intergenic | 55.0% | |
| CAAGGCAGTTGGAATGCCGA+AGG | - | Chr4:75968881-75968900 | None:intergenic | 55.0% | |
| CAGTGACATCAGCCATACCG+GGG | + | Chr4:75969276-75969295 | MsG0480022723.01.T01:CDS | 55.0% | |
| CTTGCTTGCCCCCTATGTAG+GGG | + | Chr4:75969029-75969048 | MsG0480022723.01.T01:CDS | 55.0% | |
| GGTTGCCGTCCCTATATGTG+TGG | + | Chr4:75968849-75968868 | MsG0480022723.01.T01:CDS | 55.0% | |
| TCCTTCAGCACGTCCAAGGA+CGG | + | Chr4:75969184-75969203 | MsG0480022723.01.T01:CDS | 55.0% | |
| !! | AGCACGTAATGGACAGCCTG+GGG | + | Chr4:75968935-75968954 | MsG0480022723.01.T01:CDS | 55.0% |
| !! | CAGCACGTAATGGACAGCCT+GGG | + | Chr4:75968934-75968953 | MsG0480022723.01.T01:CDS | 55.0% |
| !! | TCAGCACGTAATGGACAGCC+TGG | + | Chr4:75968933-75968952 | MsG0480022723.01.T01:CDS | 55.0% |
| ACCGTCCTTGGACGTGCTGA+AGG | - | Chr4:75969188-75969207 | None:intergenic | 60.0% | |
| CAAAACCACTGCCCCCGGTA+TGG | - | Chr4:75969291-75969310 | None:intergenic | 60.0% | |
| GCAGTTGGAATGCCGAAGGC+TGG | - | Chr4:75968877-75968896 | None:intergenic | 60.0% | |
| TTGCCCCCTATGTAGGGGTC+AGG | + | Chr4:75969034-75969053 | MsG0480022723.01.T01:CDS | 60.0% | |
| ! | CATACCGGGGGCAGTGGTTT+TGG | + | Chr4:75969289-75969308 | MsG0480022723.01.T01:CDS | 60.0% |
| !! | CGTAATGGACAGCCTGGGGA+CGG | + | Chr4:75968939-75968958 | MsG0480022723.01.T01:CDS | 60.0% |
| ATCAGCCATACCGGGGGCAG+TGG | + | Chr4:75969283-75969302 | MsG0480022723.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 75968816 | 75969325 | 75968816 | ID=MsG0480022723.01;Name=MsG0480022723.01 |
| Chr4 | mRNA | 75968816 | 75969325 | 75968816 | ID=MsG0480022723.01.T01;Parent=MsG0480022723.01;Name=MsG0480022723.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|169 |
| Chr4 | exon | 75968816 | 75969325 | 75968816 | ID=MsG0480022723.01.T01:exon:10847;Parent=MsG0480022723.01.T01 |
| Chr4 | CDS | 75968816 | 75969325 | 75968816 | ID=MsG0480022723.01.T01:cds;Parent=MsG0480022723.01.T01 |
| Gene Sequence |
| Protein sequence |
