AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022910.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022910.01.T01 MTR_8g079485 100.000 68 0 0 14 81 2 69 1.57e-43 135
MsG0480022910.01.T01 MTR_4g068040 94.118 68 4 0 14 81 2 69 7.54e-41 128
MsG0480022910.01.T01 MTR_2g018680 91.045 67 6 0 15 81 3 69 7.92e-39 123
MsG0480022910.01.T01 MTR_4g068040 95.161 62 3 0 14 75 2 63 3.75e-37 119
MsG0480022910.01.T01 MTR_2g018680 91.935 62 5 0 15 76 3 64 1.47e-35 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022910.01.T01 AT3G59540 91.176 68 6 0 14 81 2 69 2.37e-40 127
MsG0480022910.01.T01 AT2G43460 91.176 68 6 0 14 81 2 69 2.37e-40 127

Find 16 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGATGTTATGGTTAATA+TGG 0.243483 4:-78428805 MsG0480022910.01.T01:three_prime_UTR
ATTTAGCTTAACTGATGTTA+TGG 0.326795 4:-78428814 MsG0480022910.01.T01:three_prime_UTR
CTATGAGATTTGAAGATGTT+TGG 0.411953 4:-78428853 MsG0480022910.01.T01:three_prime_UTR
GAAGAAAATCCTTGATTTCG+TGG 0.412070 4:+78429172 None:intergenic
TCTTCTGACGGCGCGTAGAA+AGG 0.443174 4:-78429154 MsG0480022910.01.T01:CDS
TTGAAGCAATCTCTTCCTCC+TGG 0.482035 4:-78429021 MsG0480022910.01.T01:CDS
GGTGAAAATCAAGAGAAGTA+AGG 0.514791 4:-78429121 MsG0480022910.01.T01:CDS
GAAGCAGATCCACGAAATCA+AGG 0.537316 4:-78429181 MsG0480022910.01.T01:CDS
TGAAACTTGTAAGAAACCCT+AGG 0.539009 4:-78428938 MsG0480022910.01.T01:three_prime_UTR
GGTGAAATGTGATCATGAAA+AGG 0.555426 4:-78433505 MsG0480022910.01.T01:intron
AATTCCAAGTCACAAAACCT+AGG 0.560683 4:+78428921 None:intergenic
ATTCCAAGTCACAAAACCTA+GGG 0.568560 4:+78428922 None:intergenic
GCGTAGAAAGGACGCGAGAT+CGG 0.568899 4:-78429142 MsG0480022910.01.T01:CDS
AATCAAGAGAAGTAAGGATG+TGG 0.579591 4:-78429115 MsG0480022910.01.T01:CDS
AGATCTTGAACAGTCAAACC+AGG 0.640177 4:+78429003 None:intergenic
TCTTGAACAGTCAAACCAGG+AGG 0.741915 4:+78429006 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATAATAAATTAATT+TGG + Chr4:78430385-78430404 None:intergenic 0.0%
!! AAATTCTATAAAGTATAAAT+AGG - Chr4:78430610-78430629 MsG0480022910.01.T01:intron 10.0%
!! AACTAAAAATTATTGTAATA+AGG - Chr4:78432405-78432424 MsG0480022910.01.T01:intron 10.0%
!! TAAAAAAATGACATAATTTA+AGG - Chr4:78431834-78431853 MsG0480022910.01.T01:intron 10.0%
!! TATTTAATTAAAATAAGTAG+TGG + Chr4:78430431-78430450 None:intergenic 10.0%
!!! AAAAAGAATTACATTAATTT+TGG + Chr4:78429741-78429760 None:intergenic 10.0%
!!! ATATAATTTTTTTAATCAGT+AGG - Chr4:78430480-78430499 MsG0480022910.01.T01:intron 10.0%
!!! TATAAATTTTACAATTTTGT+TGG - Chr4:78432328-78432347 MsG0480022910.01.T01:intron 10.0%
!!! TATATTATTTTTCATCAAAT+AGG + Chr4:78431409-78431428 None:intergenic 10.0%
!!! TTTTTCATACTTTTTTTTTA+GGG + Chr4:78430651-78430670 None:intergenic 10.0%
!!! TTTTTTCATACTTTTTTTTT+AGG + Chr4:78430652-78430671 None:intergenic 10.0%
!! AAAAATACCTAAATAGTTTA+TGG + Chr4:78432372-78432391 None:intergenic 15.0%
!! AAGTATAATTTACAAAGAAA+AGG + Chr4:78429396-78429415 None:intergenic 15.0%
!! ATTTCCAAAATAACAAAATA+TGG - Chr4:78430540-78430559 MsG0480022910.01.T01:intron 15.0%
!! TAAAAAAATGATAGCAAATA+TGG + Chr4:78432648-78432667 None:intergenic 15.0%
!! TCTAATTTCAAATTTAACAA+CGG + Chr4:78431448-78431467 None:intergenic 15.0%
!! TTTCCAAAATAACAAAATAT+GGG - Chr4:78430541-78430560 MsG0480022910.01.T01:intron 15.0%
!!! AAAATTCTCAAAGTTTTTTA+AGG - Chr4:78429625-78429644 MsG0480022910.01.T01:intron 15.0%
!!! AACATTTAAAATATGAGAAA+TGG + Chr4:78430056-78430075 None:intergenic 15.0%
!!! ATTAAATTTTGGTTTTAGTA+AGG + Chr4:78431968-78431987 None:intergenic 15.0%
!!! CTTATTTATGATTGTTTTTT+TGG - Chr4:78433434-78433453 MsG0480022910.01.T01:intron 15.0%
!!! TTAAATTTTGGTTTTAGTAA+GGG + Chr4:78431967-78431986 None:intergenic 15.0%
!!! TTCTCATATTTTAAATGTTT+GGG - Chr4:78430057-78430076 MsG0480022910.01.T01:intron 15.0%
!!! TTGAAAATTTTATTCAACAT+GGG + Chr4:78430362-78430381 None:intergenic 15.0%
!!! TTTCTCATATTTTAAATGTT+TGG - Chr4:78430056-78430075 MsG0480022910.01.T01:intron 15.0%
!! AAATTAATGGAATTGACATA+AGG - Chr4:78430740-78430759 MsG0480022910.01.T01:intron 20.0%
!! AATAAAATGATGAACAGATT+TGG - Chr4:78432840-78432859 MsG0480022910.01.T01:intron 20.0%
!! AATTAATGGAATTGACATAA+GGG - Chr4:78430741-78430760 MsG0480022910.01.T01:intron 20.0%
!! ATAAAATGATGAACAGATTT+GGG - Chr4:78432841-78432860 MsG0480022910.01.T01:intron 20.0%
!! ATAATTTACAAAGAAAAGGT+TGG + Chr4:78429392-78429411 None:intergenic 20.0%
!! ATATATAATATATGCATGCA+AGG + Chr4:78432140-78432159 None:intergenic 20.0%
!! TAAAGATCCATAAACTATTT+AGG - Chr4:78432362-78432381 MsG0480022910.01.T01:intron 20.0%
!! TTCACTTTAAGCTAAATTAA+TGG - Chr4:78430727-78430746 MsG0480022910.01.T01:intron 20.0%
!!! AATTTTTTTTTTATGGTGTC+CGG - Chr4:78432100-78432119 MsG0480022910.01.T01:intron 20.0%
!!! AGGATTTGCTAATATTAAAA+TGG + Chr4:78432015-78432034 None:intergenic 20.0%
!!! AGTTAAACCTTTGTTTTTTT+TGG - Chr4:78432585-78432604 MsG0480022910.01.T01:intron 20.0%
!!! ATACCCATATTTTGTTATTT+TGG + Chr4:78430547-78430566 None:intergenic 20.0%
!!! ATTACGAGTAATATTTTAAG+AGG - Chr4:78429861-78429880 MsG0480022910.01.T01:intron 20.0%
!!! ATTCACTTTCAGATTTTTTT+TGG - Chr4:78430696-78430715 MsG0480022910.01.T01:intron 20.0%
!!! GTTGAAAATTTTATTCAACA+TGG + Chr4:78430363-78430382 None:intergenic 20.0%
!!! TAAAAGGACATAATTTGTTA+AGG + Chr4:78432305-78432324 None:intergenic 20.0%
!!! TGAAAATTTTATTCAACATG+GGG + Chr4:78430361-78430380 None:intergenic 20.0%
!!! TGTAATATATTTTCCTAATC+AGG - Chr4:78432435-78432454 MsG0480022910.01.T01:intron 20.0%
!!! TTATAATTATAAAGTACCCT+TGG - Chr4:78432665-78432684 MsG0480022910.01.T01:intron 20.0%
!!! TTGGAATTATGTTTTGTAAT+TGG - Chr4:78433409-78433428 MsG0480022910.01.T01:intron 20.0%
! AAAAGGATGGTGAAATATAA+TGG - Chr4:78429181-78429200 MsG0480022910.01.T01:CDS 25.0%
! AAACAATAATGTAGAGCTAA+TGG - Chr4:78431018-78431037 MsG0480022910.01.T01:intron 25.0%
! AACTGATGTTATGGTTAATA+TGG - Chr4:78433510-78433529 MsG0480022910.01.T01:CDS 25.0%
! ACTCTAATAAAAAATGCCAA+GGG + Chr4:78432684-78432703 None:intergenic 25.0%
! ACTTTCCTTTGTAAATTAGT+GGG + Chr4:78429929-78429948 None:intergenic 25.0%
! AGTATGGAGAAAACAAAAAT+GGG - Chr4:78429510-78429529 MsG0480022910.01.T01:intron 25.0%
! ATACAAGTTGTCAACTAAAT+TGG - Chr4:78429419-78429438 MsG0480022910.01.T01:intron 25.0%
! ATCCTAATAAAACTATTGTG+CGG - Chr4:78432030-78432049 MsG0480022910.01.T01:intron 25.0%
! CTGTTCTATTATTTGTGAAT+CGG - Chr4:78431081-78431100 MsG0480022910.01.T01:intron 25.0%
! TACTCTAATAAAAAATGCCA+AGG + Chr4:78432685-78432704 None:intergenic 25.0%
! TACTTTCCTTTGTAAATTAG+TGG + Chr4:78429930-78429949 None:intergenic 25.0%
! TATAATAGAATGACCTGATT+AGG + Chr4:78432451-78432470 None:intergenic 25.0%
!! AAAAGGTATAGGTATTACAA+GGG - Chr4:78428827-78428846 MsG0480022910.01.T01:three_prime_UTR 25.0%
!! AATTGACATAAGGGTATAAT+AGG - Chr4:78430750-78430769 MsG0480022910.01.T01:intron 25.0%
!! ACATATACTGATCATTGTTT+TGG - Chr4:78432275-78432294 MsG0480022910.01.T01:intron 25.0%
!! ATTTAGCTTAACTGATGTTA+TGG - Chr4:78433501-78433520 MsG0480022910.01.T01:intron 25.0%
!! CATATACTGATCATTGTTTT+GGG - Chr4:78432276-78432295 MsG0480022910.01.T01:intron 25.0%
!! GAAAATTTTATTCAACATGG+GGG + Chr4:78430360-78430379 None:intergenic 25.0%
!! TAGTATTTTCTCCGTTATTT+AGG - Chr4:78431732-78431751 MsG0480022910.01.T01:intron 25.0%
!! TTTAAGGTAATCATAAGTGT+CGG - Chr4:78431850-78431869 MsG0480022910.01.T01:intron 25.0%
!!! AAACCTTTGTTTTTTTTGGA+AGG - Chr4:78432589-78432608 MsG0480022910.01.T01:intron 25.0%
!!! AACCTTTGTTTTTTTTGGAA+GGG - Chr4:78432590-78432609 MsG0480022910.01.T01:intron 25.0%
!!! ATGTATTGTGTTGTTTTTTC+AGG - Chr4:78433110-78433129 MsG0480022910.01.T01:intron 25.0%
!!! GTTGAAAATGTCATTTTTGT+AGG + Chr4:78429719-78429738 None:intergenic 25.0%
AAAGATTGAAGTGTGAGTTT+CGG + Chr4:78431500-78431519 None:intergenic 30.0%
AAATAGAGAAGAGAGAGATA+AGG + Chr4:78430929-78430948 None:intergenic 30.0%
AAGGAAAGTAGATAGTTACA+CGG - Chr4:78429940-78429959 MsG0480022910.01.T01:intron 30.0%
AATCAATTATTCTCGCAAAG+GGG - Chr4:78429773-78429792 MsG0480022910.01.T01:intron 30.0%
ACTCACACATTCTTTGTAAA+GGG - Chr4:78431224-78431243 MsG0480022910.01.T01:intron 30.0%
AGAAATAGAGTAGATTTGAG+GGG - Chr4:78430260-78430279 MsG0480022910.01.T01:intron 30.0%
AGATAAAGAATAGTCCAGTA+TGG - Chr4:78429494-78429513 MsG0480022910.01.T01:intron 30.0%
AGCCCAAAATGTAATTCATA+TGG + Chr4:78432877-78432896 None:intergenic 30.0%
AGGTATTACAAGGGTAAATT+TGG - Chr4:78428836-78428855 MsG0480022910.01.T01:three_prime_UTR 30.0%
ATTGACGTTTGTTCAATTCA+GGG - Chr4:78429027-78429046 MsG0480022910.01.T01:CDS 30.0%
CAGTATGGAGAAAACAAAAA+TGG - Chr4:78429509-78429528 MsG0480022910.01.T01:intron 30.0%
CTATGAGATTTGAAGATGTT+TGG - Chr4:78433462-78433481 MsG0480022910.01.T01:intron 30.0%
GAAAGAATTGAGAGCAAATT+TGG + Chr4:78429588-78429607 None:intergenic 30.0%
GAATCAATTATTCTCGCAAA+GGG - Chr4:78429772-78429791 MsG0480022910.01.T01:intron 30.0%
GAGAAATAGAGTAGATTTGA+GGG - Chr4:78430259-78430278 MsG0480022910.01.T01:intron 30.0%
GAGAGATAAAATTGTCACAT+AGG - Chr4:78430005-78430024 MsG0480022910.01.T01:intron 30.0%
GATCAGTAATAAATATACCC+AGG - Chr4:78430153-78430172 MsG0480022910.01.T01:intron 30.0%
GATGACATATTTGTCTAGTT+TGG + Chr4:78429695-78429714 None:intergenic 30.0%
GCAAATTTAAGGACGAATTT+GGG - Chr4:78429098-78429117 MsG0480022910.01.T01:CDS 30.0%
TACTCACACATTCTTTGTAA+AGG - Chr4:78431223-78431242 MsG0480022910.01.T01:intron 30.0%
TCCTAATAAAACTATTGTGC+GGG - Chr4:78432031-78432050 MsG0480022910.01.T01:intron 30.0%
TGAATCAATTATTCTCGCAA+AGG - Chr4:78429771-78429790 MsG0480022910.01.T01:intron 30.0%
TGAGAAATAGAGTAGATTTG+AGG - Chr4:78430258-78430277 MsG0480022910.01.T01:intron 30.0%
TGAGTACTTATGCAGAAAAA+CGG + Chr4:78430107-78430126 None:intergenic 30.0%
TGCAAATTTAAGGACGAATT+TGG - Chr4:78429097-78429116 MsG0480022910.01.T01:CDS 30.0%
TTGGCTATATCTCAAACTAT+AGG + Chr4:78429676-78429695 None:intergenic 30.0%
TTTACTTTCTCCTAATGCTT+TGG + Chr4:78429969-78429988 None:intergenic 30.0%
TTTGGGTATTCACTCATATT+TGG - Chr4:78429115-78429134 MsG0480022910.01.T01:CDS 30.0%
TTTGTGCGTTTCAATCAAAT+TGG - Chr4:78428898-78428917 MsG0480022910.01.T01:three_prime_UTR 30.0%
! AAAACCAAGTTTCTTTTGAG+AGG + Chr4:78429461-78429480 None:intergenic 30.0%
! AAATATACCCAGGAACTTTT+AGG - Chr4:78430163-78430182 MsG0480022910.01.T01:intron 30.0%
! AATATACCCAGGAACTTTTA+GGG - Chr4:78430164-78430183 MsG0480022910.01.T01:intron 30.0%
! AATCAAGGATTTTCTTCTGA+CGG - Chr4:78433149-78433168 MsG0480022910.01.T01:intron 30.0%
! ATGTGATCATGAAAAGGTAT+AGG - Chr4:78428816-78428835 MsG0480022910.01.T01:three_prime_UTR 30.0%
! CTCTAGACAATTTTTACTCA+AGG + Chr4:78432561-78432580 None:intergenic 30.0%
! GAAAAGGTATAGGTATTACA+AGG - Chr4:78428826-78428845 MsG0480022910.01.T01:three_prime_UTR 30.0%
! GGCCATATGAATTACATTTT+GGG - Chr4:78432872-78432891 MsG0480022910.01.T01:intron 30.0%
! TGAATCTACAACGATGATTT+TGG - Chr4:78433073-78433092 MsG0480022910.01.T01:intron 30.0%
! TGAATTACATTTTGGGCTTT+AGG - Chr4:78432879-78432898 MsG0480022910.01.T01:intron 30.0%
! TTTGGAATAAGCTTCAGAAA+TGG + Chr4:78432956-78432975 None:intergenic 30.0%
!! TTAACCTTTTCTTTTTGGTG+CGG - Chr4:78432615-78432634 MsG0480022910.01.T01:intron 30.0%
!! TTTGGTAGTGAAGAGAAATA+GGG + Chr4:78430869-78430888 None:intergenic 30.0%
!!! CATTTTTGTTTTCTCCATAC+TGG + Chr4:78429511-78429530 None:intergenic 30.0%
AAGCGTGCATTTCGAATAAT+TGG - Chr4:78429053-78429072 MsG0480022910.01.T01:CDS 35.0%
AATCAAGAGAAGTAAGGATG+TGG - Chr4:78433200-78433219 MsG0480022910.01.T01:intron 35.0%
AATTCCAAGTCACAAAACCT+AGG + Chr4:78433397-78433416 None:intergenic 35.0%
AGCGTGCATTTCGAATAATT+GGG - Chr4:78429054-78429073 MsG0480022910.01.T01:CDS 35.0%
AGTTGCAAGGAATAAAAGGA+TGG - Chr4:78429168-78429187 MsG0480022910.01.T01:CDS 35.0%
ATTCCAAGTCACAAAACCTA+GGG + Chr4:78433396-78433415 None:intergenic 35.0%
ATTTCTCTTCACTACCAAAC+AGG - Chr4:78430870-78430889 MsG0480022910.01.T01:intron 35.0%
CCTGGTTCAAACCTAAATAA+CGG + Chr4:78431746-78431765 None:intergenic 35.0%
CTCCAATTAACGAAAGAAGT+GGG + Chr4:78433033-78433052 None:intergenic 35.0%
CTCCCTTCCAAAAAAAACAA+AGG + Chr4:78432595-78432614 None:intergenic 35.0%
GAAACTCACACTTCAATCTT+TGG - Chr4:78431499-78431518 MsG0480022910.01.T01:intron 35.0%
GAAGAAAATCCTTGATTTCG+TGG + Chr4:78433146-78433165 None:intergenic 35.0%
GAAGAGAGACTAAAAACATG+TGG + Chr4:78430847-78430866 None:intergenic 35.0%
GAAGTTGTGAAGAGAGAAAT+AGG - Chr4:78430205-78430224 MsG0480022910.01.T01:intron 35.0%
GATAGTAAAGTTACCCTGTT+TGG + Chr4:78430887-78430906 None:intergenic 35.0%
GATTGACGTTTGTTCAATTC+AGG - Chr4:78429026-78429045 MsG0480022910.01.T01:CDS 35.0%
GGTGAAAATCAAGAGAAGTA+AGG - Chr4:78433194-78433213 MsG0480022910.01.T01:intron 35.0%
GGTGAAATGTGATCATGAAA+AGG - Chr4:78428810-78428829 MsG0480022910.01.T01:three_prime_UTR 35.0%
GTCCTTAAATTTGCACGAAT+CGG + Chr4:78429092-78429111 None:intergenic 35.0%
GTGAAGTTGCAAGGAATAAA+AGG - Chr4:78429164-78429183 MsG0480022910.01.T01:CDS 35.0%
TAATATATGCATGCAAGGTC+CGG + Chr4:78432135-78432154 None:intergenic 35.0%
TCGAGTTAAGCATATGCAAT+GGG + Chr4:78431574-78431593 None:intergenic 35.0%
TCTCCAAAACTGAGAAGAAA+TGG + Chr4:78430804-78430823 None:intergenic 35.0%
TGAAACTTGTAAGAAACCCT+AGG - Chr4:78433377-78433396 MsG0480022910.01.T01:intron 35.0%
TGACACCCACTAATTTACAA+AGG - Chr4:78429921-78429940 MsG0480022910.01.T01:intron 35.0%
TGGAATTGTATACAAGAGTG+TGG + Chr4:78429542-78429561 None:intergenic 35.0%
TGGAGAAAACAAAAATGGGA+TGG - Chr4:78429514-78429533 MsG0480022910.01.T01:intron 35.0%
TTCCCACTTCTTTCGTTAAT+TGG - Chr4:78433028-78433047 MsG0480022910.01.T01:intron 35.0%
TTTCTCTTCACTACCAAACA+GGG - Chr4:78430871-78430890 MsG0480022910.01.T01:intron 35.0%
! ACAACGGATTTCTCACTTAT+AGG + Chr4:78431432-78431451 None:intergenic 35.0%
! CTTCCATTTCTTCTCAGTTT+TGG - Chr4:78430798-78430817 MsG0480022910.01.T01:intron 35.0%
! GGAAGCATATTATGATCACA+TGG - Chr4:78429794-78429813 MsG0480022910.01.T01:intron 35.0%
! GGAGAGAAAGAATTTTGTGT+GGG - Chr4:78430819-78430838 MsG0480022910.01.T01:intron 35.0%
! GGGAGTTAACCTTTTCTTTT+TGG - Chr4:78432610-78432629 MsG0480022910.01.T01:intron 35.0%
! GGGCCATATGAATTACATTT+TGG - Chr4:78432871-78432890 MsG0480022910.01.T01:intron 35.0%
! TAGTTTGAGACTATGTTGCA+CGG + Chr4:78431928-78431947 None:intergenic 35.0%
! TGGAGAGAAAGAATTTTGTG+TGG - Chr4:78430818-78430837 MsG0480022910.01.T01:intron 35.0%
!! CTTTGATGGTGGAAAATCAA+TGG + Chr4:78429562-78429581 None:intergenic 35.0%
!! GTTTGGTAGTGAAGAGAAAT+AGG + Chr4:78430870-78430889 None:intergenic 35.0%
!! TTCTGAAGCTTATTCCAAAG+AGG - Chr4:78432957-78432976 MsG0480022910.01.T01:intron 35.0%
!! TTTGGGCTTTAGGACAAAAT+CGG - Chr4:78432889-78432908 MsG0480022910.01.T01:intron 35.0%
!!! ACCCGCACAATAGTTTTATT+AGG + Chr4:78432035-78432054 None:intergenic 35.0%
!!! CATTGATTTTCCACCATCAA+AGG - Chr4:78429560-78429579 MsG0480022910.01.T01:intron 35.0%
!!! TTAGTCGACGTTGCTTTTTT+TGG - Chr4:78431108-78431127 MsG0480022910.01.T01:intron 35.0%
AAAAACAGATGGGAGAAGAG+CGG + Chr4:78432828-78432809 None:intergenic 40.0%
AAACACACCCTAAAAGTTCC+TGG + Chr4:78430174-78430193 None:intergenic 40.0%
AACACACCCTAAAAGTTCCT+GGG + Chr4:78430173-78430192 None:intergenic 40.0%
AACTCCGCACCAAAAAGAAA+AGG + Chr4:78432622-78432641 None:intergenic 40.0%
AATATATGCATGCAAGGTCC+GGG + Chr4:78432134-78432153 None:intergenic 40.0%
ACCATTGATGTGGTTTCTTG+TGG + Chr4:78433006-78433025 None:intergenic 40.0%
AGATCTTGAACAGTCAAACC+AGG + Chr4:78433315-78433334 None:intergenic 40.0%
AGTAGATTTGAGGGGTTGTT+TGG - Chr4:78430268-78430287 MsG0480022910.01.T01:intron 40.0%
ATAAACCCTACTCGTCAACT+CGG + Chr4:78432919-78432938 None:intergenic 40.0%
CAAATTGGTCCTTGTATACG+TGG - Chr4:78428913-78428932 MsG0480022910.01.T01:three_prime_UTR 40.0%
CACTCCTCTCAAAAGAAACT+TGG - Chr4:78429454-78429473 MsG0480022910.01.T01:intron 40.0%
CGAAGAATTGTGAAGTTGCA+AGG - Chr4:78429155-78429174 MsG0480022910.01.T01:CDS 40.0%
CGTGCATTTCGAATAATTGG+GGG - Chr4:78429056-78429075 MsG0480022910.01.T01:CDS 40.0%
CTACCAAACACAGTGTTAGT+GGG - Chr4:78430954-78430973 MsG0480022910.01.T01:intron 40.0%
CTCTTCATGTTGAAAACCGA+TGG + Chr4:78431166-78431185 None:intergenic 40.0%
GAAATAGGTGAGAGAGAGTA+TGG - Chr4:78430220-78430239 MsG0480022910.01.T01:intron 40.0%
GCAAGTGAGCAAAAACAGAT+GGG - Chr4:78432799-78432818 MsG0480022910.01.T01:intron 40.0%
GCGTGCATTTCGAATAATTG+GGG - Chr4:78429055-78429074 MsG0480022910.01.T01:CDS 40.0%
GCTCCAATTAACGAAAGAAG+TGG + Chr4:78433034-78433053 None:intergenic 40.0%
GGGAATGAGCAAAAACAGAT+GGG + Chr4:78432718-78432737 None:intergenic 40.0%
GGTCATATATGTGTTTGTCG+AGG + Chr4:78431770-78431789 None:intergenic 40.0%
GTAATTCATATGGCCCAACA+CGG + Chr4:78432867-78432886 None:intergenic 40.0%
GTCGAGTTAAGCATATGCAA+TGG + Chr4:78431575-78431594 None:intergenic 40.0%
GTGTTAGACACGTCACATAT+CGG - Chr4:78431872-78431891 MsG0480022910.01.T01:intron 40.0%
GTTTCTTGTGGCTGCAAATA+GGG + Chr4:78432994-78433013 None:intergenic 40.0%
TACCAAACACAGTGTTAGTG+GGG - Chr4:78430955-78430974 MsG0480022910.01.T01:intron 40.0%
TACCAACTGAGCTAAACTCA+CGG - Chr4:78432192-78432211 MsG0480022910.01.T01:intron 40.0%
TCTACCAAACACAGTGTTAG+TGG - Chr4:78430953-78430972 MsG0480022910.01.T01:intron 40.0%
TGAAAACCGATGGTGCATAT+GGG + Chr4:78431156-78431175 None:intergenic 40.0%
TTGAAAACCGATGGTGCATA+TGG + Chr4:78431157-78431176 None:intergenic 40.0%
TTGTAAAGGGAATGCTTGTG+CGG - Chr4:78431237-78431256 MsG0480022910.01.T01:intron 40.0%
! AAATTTGGCTCCTTTGATGG+TGG + Chr4:78429573-78429592 None:intergenic 40.0%
! AGCAAATTTGGCTCCTTTGA+TGG + Chr4:78429576-78429595 None:intergenic 40.0%
! CCGTTATTTAGGTTTGAACC+AGG - Chr4:78431743-78431762 MsG0480022910.01.T01:intron 40.0%
! GAATAATTGGGGGACGTTTT+TGG - Chr4:78429066-78429085 MsG0480022910.01.T01:CDS 40.0%
! TACACGGAAACCAAAGCATT+AGG - Chr4:78429956-78429975 MsG0480022910.01.T01:intron 40.0%
! TGAGTTTAGCTCAGTTGGTA+AGG + Chr4:78432192-78432211 None:intergenic 40.0%
! TTAGATATGGCTTGGAATGC+TGG - Chr4:78431055-78431074 MsG0480022910.01.T01:intron 40.0%
! TTCTGATTGAAGGTGTGTTC+TGG + Chr4:78431901-78431920 None:intergenic 40.0%
! TTTGGTTGCTTTGGCTATTG+TGG - Chr4:78430986-78431005 MsG0480022910.01.T01:intron 40.0%
!! AAACCCTAGGTTTTGTGACT+TGG - Chr4:78433390-78433409 MsG0480022910.01.T01:intron 40.0%
!! ACTATTGTGCGGGTTGTTTT+TGG - Chr4:78432041-78432060 MsG0480022910.01.T01:intron 40.0%
!! CTCACGCTCAGCTATTTTAA+CGG + Chr4:78432739-78432758 None:intergenic 40.0%
!! TTGGGCTTTAGGACAAAATC+GGG - Chr4:78432890-78432909 MsG0480022910.01.T01:intron 40.0%
!!! AGGAACTTTTAGGGTGTGTT+TGG - Chr4:78430173-78430192 MsG0480022910.01.T01:intron 40.0%
!!! CATCGGTTTTCAACATGAAG+AGG - Chr4:78431164-78431183 MsG0480022910.01.T01:intron 40.0%
!!! CTATTGTGCGGGTTGTTTTT+GGG - Chr4:78432042-78432061 MsG0480022910.01.T01:intron 40.0%
!!! CTTTTAGGGTGTGTTTGGTT+TGG - Chr4:78430178-78430197 MsG0480022910.01.T01:intron 40.0%
!!! TCACGCTCAGCTATTTTAAC+GGG + Chr4:78432738-78432757 None:intergenic 40.0%
AAGCTTATTCCAAAGAGGCG+AGG - Chr4:78432962-78432981 MsG0480022910.01.T01:intron 45.0%
ACAAGGAAGCCACGTATACA+AGG + Chr4:78428925-78428944 None:intergenic 45.0%
ACCAACTGAGCTAAACTCAC+GGG - Chr4:78432193-78432212 MsG0480022910.01.T01:intron 45.0%
AGACAACTCAGCACCACTAA+CGG - Chr4:78428978-78428997 MsG0480022910.01.T01:three_prime_UTR 45.0%
ATATATGCATGCAAGGTCCG+GGG + Chr4:78432133-78432152 None:intergenic 45.0%
ATATGTGTTTGTCGAGGTCC+TGG + Chr4:78431764-78431783 None:intergenic 45.0%
CGCAAGTGAGCAAAAACAGA+TGG - Chr4:78432798-78432817 MsG0480022910.01.T01:intron 45.0%
CGGGAATGAGCAAAAACAGA+TGG + Chr4:78432719-78432738 None:intergenic 45.0%
GAAGCAGATCCACGAAATCA+AGG - Chr4:78433134-78433153 MsG0480022910.01.T01:intron 45.0%
GCCACAAGAAACCACATCAA+TGG - Chr4:78433002-78433021 MsG0480022910.01.T01:intron 45.0%
GGTTTCTTGTGGCTGCAAAT+AGG + Chr4:78432995-78433014 None:intergenic 45.0%
TAAACCCTACTCGTCAACTC+GGG + Chr4:78432918-78432937 None:intergenic 45.0%
TATGCAGAAAAACGGCACAC+AGG + Chr4:78430099-78430118 None:intergenic 45.0%
TCCAAGCCATATCTAAGTCG+CGG + Chr4:78431051-78431070 None:intergenic 45.0%
TCTTGAACAGTCAAACCAGG+AGG + Chr4:78433312-78433331 None:intergenic 45.0%
TGCTGAGTTGTCTTCTTGCA+TGG + Chr4:78428971-78428990 None:intergenic 45.0%
TTGAAGCAATCTCTTCCTCC+TGG - Chr4:78433294-78433313 MsG0480022910.01.T01:intron 45.0%
! CCACTAACGGAGCATTTAGA+CGG - Chr4:78428991-78429010 MsG0480022910.01.T01:exon 45.0%
! TAACGGAGCATTTAGACGGA+AGG - Chr4:78428995-78429014 MsG0480022910.01.T01:exon 45.0%
! TGTCTTCTTGCATGGCTTGT+TGG + Chr4:78428963-78428982 None:intergenic 45.0%
!! AGCTCAGTTGGTAAGGACAA+TGG + Chr4:78432185-78432204 None:intergenic 45.0%
!! AGTGGGAAGTACCATTGATG+TGG + Chr4:78433016-78433035 None:intergenic 45.0%
!! GGCCGATTCGTGCAAATTTA+AGG - Chr4:78429087-78429106 MsG0480022910.01.T01:CDS 45.0%
!!! GTCGACGTTGCTTTTTTTGG+AGG - Chr4:78431111-78431130 MsG0480022910.01.T01:intron 45.0%
!! TGTAAAATTTATAAAATAAA+AGG + Chr4:78432321-78432340 None:intergenic 5.0%
!! TTAATTTATTTGTAATTTAT+AGG + Chr4:78432256-78432275 None:intergenic 5.0%
!!! ATTTTAGTTTTATTAAATTT+TGG + Chr4:78431979-78431998 None:intergenic 5.0%
!!! CTAAAATAATTTTTTTTTTA+TGG - Chr4:78432093-78432112 MsG0480022910.01.T01:intron 5.0%
!!! TCATTTTTTTAAAATTTTAT+CGG + Chr4:78431825-78431844 None:intergenic 5.0%
AACACAGTGTTAGTGGGGAG+TGG - Chr4:78430960-78430979 MsG0480022910.01.T01:intron 50.0%
AACCGACCGCGACTTAGATA+TGG - Chr4:78431042-78431061 MsG0480022910.01.T01:intron 50.0%
AAGGGAATGCTTGTGCGGAT+TGG - Chr4:78431242-78431261 MsG0480022910.01.T01:intron 50.0%
ACCGCGACTTAGATATGGCT+TGG - Chr4:78431047-78431066 MsG0480022910.01.T01:intron 50.0%
AGCCATATCTAAGTCGCGGT+CGG + Chr4:78431047-78431066 None:intergenic 50.0%
CCGTCTAAATGCTCCGTTAG+TGG + Chr4:78428994-78429013 None:intergenic 50.0%
CTCCCCACTAACACTGTGTT+TGG + Chr4:78430960-78430979 None:intergenic 50.0%
GAACAGATTTGGGCCGTGTT+GGG - Chr4:78432851-78432870 MsG0480022910.01.T01:intron 50.0%
GAGTGGCTGTTTGGTTGCTT+TGG - Chr4:78430977-78430996 MsG0480022910.01.T01:intron 50.0%
GCACGGACACTTCTGATTGA+AGG + Chr4:78431911-78431930 None:intergenic 50.0%
GGCTTGTACACGTGTCTACA+AGG + Chr4:78428942-78428961 None:intergenic 50.0%
TGAACAGATTTGGGCCGTGT+TGG - Chr4:78432850-78432869 MsG0480022910.01.T01:intron 50.0%
! TCCCGTGAGTTTAGCTCAGT+TGG + Chr4:78432197-78432216 None:intergenic 50.0%
!!! GGGTTTTTTCCTCGCCTCTT+TGG + Chr4:78432974-78432993 None:intergenic 50.0%
CGTGTCAGTGTCGTGTTCGA+TGG + Chr4:78431791-78431810 None:intergenic 55.0%
GACTCGCCCATATGCACCAT+CGG - Chr4:78431147-78431166 MsG0480022910.01.T01:intron 55.0%
GAGCGTGAGTTAACTCACGC+AGG - Chr4:78432750-78432769 MsG0480022910.01.T01:intron 55.0%
GCGTAGAAAGGACGCGAGAT+CGG - Chr4:78433173-78433192 MsG0480022910.01.T01:intron 55.0%
GGTGCATATGGGCGAGTCAA+GGG + Chr4:78431145-78431164 None:intergenic 55.0%
GTGCATATGGGCGAGTCAAG+GGG + Chr4:78431144-78431163 None:intergenic 55.0%
GTTAGTGGGGAGTGGCTGTT+TGG - Chr4:78430968-78430987 MsG0480022910.01.T01:intron 55.0%
TCTTCTGACGGCGCGTAGAA+AGG - Chr4:78433161-78433180 MsG0480022910.01.T01:intron 55.0%
TGGTGCATATGGGCGAGTCA+AGG + Chr4:78431146-78431165 None:intergenic 55.0%
! GGTACCCGAGTTGACGAGTA+GGG - Chr4:78432911-78432930 MsG0480022910.01.T01:intron 55.0%
CAAGGTCCGGGGTTCGAATC+CGG + Chr4:78432122-78432141 None:intergenic 60.0%
CATATGGGCGAGTCAAGGGG+TGG + Chr4:78431141-78431160 None:intergenic 60.0%
TGGTGTCCGGATTCGAACCC+CGG - Chr4:78432113-78432132 MsG0480022910.01.T01:intron 60.0%
! GGGTACCCGAGTTGACGAGT+AGG - Chr4:78432910-78432929 MsG0480022910.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 78428793 78433544 78428793 ID=MsG0480022910.01;Name=MsG0480022910.01
Chr4 mRNA 78428793 78433544 78428793 ID=MsG0480022910.01.T01;Parent=MsG0480022910.01;Name=MsG0480022910.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0.5|0.5|1|1|2|206|81
Chr4 exon 78428793 78429205 78428793 ID=MsG0480022910.01.T01:exon:23490;Parent=MsG0480022910.01.T01
Chr4 exon 78433506 78433544 78433506 ID=MsG0480022910.01.T01:exon:23489;Parent=MsG0480022910.01.T01
Chr4 CDS 78433506 78433544 78433506 ID=MsG0480022910.01.T01:cds;Parent=MsG0480022910.01.T01
Chr4 CDS 78428999 78429205 78428999 ID=MsG0480022910.01.T01:cds;Parent=MsG0480022910.01.T01
Chr4 three_prime_UTR 78428793 78428998 78428793 ID=MsG0480022910.01.T01:three_prime_utr;Parent=MsG0480022910.01.T01
Gene Sequence

>MsG0480022910.01.T01

ATGTTTTTGATGTTCTGGGTGAAATGTGATCATGAAAAGGCGAAGCAGATCCACGAAATCAAGGATTTTCTTCTGACGGCGCGTAGAAAGGACGCGAGATCGGTGAAAATCAAGAGAAGTAAGGATGTGGTGAAGTTCAAAGTTCGTTGCTCAAAGTATTTGTACACACTTTGTGTGTTTGATACTGACAAAGCTGATAAGTTGAAGCAATCTCTTCCTCCTGGTTTGACTGTTCAAGATCTTTAG
Protein sequence

>MsG0480022910.01.T01

MFLMFWVKCDHEKAKQIHEIKDFLLTARRKDARSVKIKRSKDVVKFKVRCSKYLYTLCVFDTDKADKLKQSLPPGLTVQDL*