AlfalfaGEDB | MsG0480023399.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023399.01

KEGG: K11088
GO: GO:0000387 GO:0005681
PFAM: PF01423

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023399.01.T01	XP_003630137.1	100	131	0	0	1	131	1	131	4.50E-91	270

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023399.01.T01	Q9LM92	84.091	132	19	2	1	131	1	131	1.11E-70	211

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023399.01.T01	A0A2K3NP25	100.000	131	0	0	1	131	1	131	2.15e-91	270

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048135.01	MsG0480023399.01	0.865935	4.116519e-65	1.362999e-61
MsG0080048687.01	MsG0480023399.01	0.831869	1.307176e-55	1.436910e-52
MsG0080048971.01	MsG0480023399.01	0.843895	1.057237e-58	1.678281e-55
MsG0180000575.01	MsG0480023399.01	0.907325	5.722687e-81	1.010402e-76
MsG0180000614.01	MsG0480023399.01	0.854591	1.111839e-61	2.495801e-58
MsG0180000615.01	MsG0480023399.01	0.863943	1.734342e-64	5.351475e-61
MsG0180000743.01	MsG0480023399.01	0.928622	2.226587e-92	1.187026e-87
MsG0180003302.01	MsG0480023399.01	0.812907	3.346600e-51	2.154275e-48
MsG0180003409.01	MsG0480023399.01	0.807768	4.301887e-50	2.416677e-47
MsG0180003612.01	MsG0480023399.01	0.803914	2.776940e-49	1.412748e-46

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PPI

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Gene1	Gene2	Type
MsG0180001832.01	MsG0480023399.01	PPI
MsG0180006196.01	MsG0480023399.01	PPI
MsG0280009166.01	MsG0480023399.01	PPI
MsG0280010778.01	MsG0480023399.01	PPI
MsG0380016513.01	MsG0480023399.01	PPI
MsG0480019597.01	MsG0480023399.01	PPI
MsG0480022705.01	MsG0480023399.01	PPI
MsG0480023399.01	MsG0480023962.01	PPI
MsG0480023399.01	MsG0880046760.01	PPI
MsG0480023399.01	MsG0280009425.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023399.01.T01	MTR_8g092200	100.000	131	0	0	1	131	1	131	5.45e-95	270
MsG0480023399.01.T01	MTR_8g058537	36.111	72	45	1	7	77	2	73	1.12e-12	61.2
MsG0480023399.01.T01	MTR_8g058420	36.111	72	45	1	7	77	2	73	1.12e-12	61.2
MsG0480023399.01.T01	MTR_8g091620	36.111	72	45	1	7	77	2	73	1.45e-12	61.2
MsG0480023399.01.T01	MTR_7g108700	36.111	72	45	1	7	77	2	73	1.65e-12	60.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023399.01.T01	AT1G20580	84.091	132	19	2	1	131	1	131	1.14e-71	211
MsG0480023399.01.T01	AT1G76300	77.863	131	26	2	1	131	1	128	7.85e-69	203
MsG0480023399.01.T01	AT5G27720	36.111	72	45	1	7	77	2	73	2.56e-12	60.1

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Find 54 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGATGAGTCGTAGTT+TGG	0.344374	4:-84692854	MsG0480023399.01.T01:exon
AAGAAGATGAGTCGTAGTTT+GGG	0.420399	4:-84692853	MsG0480023399.01.T01:exon
AAGAGCGGCGAACTCTACAG+AGG	0.686815	4:-84692775	MsG0480023399.01.T01:CDS
AAGCATCACTTACACTGCTA+AGG	0.553810	4:-84692710	MsG0480023399.01.T01:intron
AAGCTCCTCCACGAGGCCTC+CGG	0.500533	4:-84692820	MsG0480023399.01.T01:CDS
AAGGGCAAGGGTGCATCGCT+TGG	0.512282	4:-84691299	MsG0480023399.01.T01:CDS
AAGGGTGCATCGCTTGGTGT+TGG	0.544000	4:-84691293	MsG0480023399.01.T01:CDS
AAGTTATTATGAGTTTCGTG+AGG	0.640457	4:-84690838	MsG0480023399.01.T01:three_prime_UTR
AATGATGATCACCTCCTCAC+TGG	0.558350	4:+84691063	None:intergenic
ACCAGCCAAGCACACAATAC+AGG	0.498191	4:+84690911	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTTATTATGAGTTTCGTG+AGG	-	Chr4:84692874-84692893	MsG0480023399.01.T01:five_prime_UTR	30.0%
AGTTATTATGAGTTTCGTGA+GGG	-	Chr4:84692875-84692894	MsG0480023399.01.T01:five_prime_UTR	30.0%
ATACTAATGTGACAGGTTTA+TGG	-	Chr4:84692249-84692268	MsG0480023399.01.T01:intron	30.0%
GTTGAAAAAGATCGTGAATT+AGG	-	Chr4:84690817-84690836	MsG0480023399.01.T01:three_prime_UTR	30.0%
TAAATCGACCCATAAAAGTT+AGG	-	Chr4:84691352-84691371	MsG0480023399.01.T01:intron	30.0%
TAGGGATCTTTGAATACTTA+TGG	-	Chr4:84691182-84691201	MsG0480023399.01.T01:intron	30.0%
TATCAAACTAAGCAATAAGC+TGG	+	Chr4:84691496-84691515	None:intergenic	30.0%
TGGATTGAATTTGTAGTGTT+TGG	+	Chr4:84691708-84691727	None:intergenic	30.0%
TTACACCACATTTGTAACAA+CGG	+	Chr4:84692563-84692582	None:intergenic	30.0%
AAAGAAGATGAGTCGTAGTT+TGG	-	Chr4:84690858-84690877	MsG0480023399.01.T01:three_prime_UTR	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	84690765	84692969	84690765	ID=MsG0480023399.01;Name=MsG0480023399.01
Chr4	mRNA	84690765	84692969	84690765	ID=MsG0480023399.01.T01;Parent=MsG0480023399.01;Name=MsG0480023399.01.T01;_AED=0.49;_eAED=0.49;_QI=100\|1\|1\|1\|1\|1\|5\|306\|131
Chr4	exon	84690765	84691130	84690765	ID=MsG0480023399.01.T01:exon:6180;Parent=MsG0480023399.01.T01
Chr4	exon	84691262	84691318	84691262	ID=MsG0480023399.01.T01:exon:6179;Parent=MsG0480023399.01.T01
Chr4	exon	84691404	84691470	84691404	ID=MsG0480023399.01.T01:exon:6178;Parent=MsG0480023399.01.T01
Chr4	exon	84691587	84691639	84691587	ID=MsG0480023399.01.T01:exon:6177;Parent=MsG0480023399.01.T01
Chr4	exon	84692711	84692969	84692711	ID=MsG0480023399.01.T01:exon:6176;Parent=MsG0480023399.01.T01
Chr4	five_prime_UTR	84692870	84692969	84692870	ID=MsG0480023399.01.T01:five_prime_utr;Parent=MsG0480023399.01.T01
Chr4	CDS	84692711	84692869	84692711	ID=MsG0480023399.01.T01:cds;Parent=MsG0480023399.01.T01
Chr4	CDS	84691587	84691639	84691587	ID=MsG0480023399.01.T01:cds;Parent=MsG0480023399.01.T01
Chr4	CDS	84691404	84691470	84691404	ID=MsG0480023399.01.T01:cds;Parent=MsG0480023399.01.T01
Chr4	CDS	84691262	84691318	84691262	ID=MsG0480023399.01.T01:cds;Parent=MsG0480023399.01.T01
Chr4	CDS	84691071	84691130	84691071	ID=MsG0480023399.01.T01:cds;Parent=MsG0480023399.01.T01
Chr4	three_prime_UTR	84690765	84691070	84690765	ID=MsG0480023399.01.T01:three_prime_utr;Parent=MsG0480023399.01.T01

Gene Sequence

>MsG0480023399.01.T01

ATGAGTCGTAGTTTGGGAATTCCGGTGAAGCTCCTCCACGAGGCCTCCGGCCACGTCGTCACGGTGGAGCTCAAGAGCGGCGAACTCTACAGAGGCAACATGATTGAGTGTGAGGATAACTGGAACTGCCAACTCGAAAGCATCACTTACACTGCTAAGGATGGAAAAACGTCACAACTTGAGCATGTATTTATTCGAGGTAGCAAAGTCAGGTTTATGGTCATACCCGACATGCTGAAGAACGCTCCCATGTTCAAGCGTTTAGATGCAAGAATCAAGGGCAAGGGTGCATCGCTTGGTGTTGGTAGAGGCAGAGCAGTTGCAATGCGAGCAAAGGCTCAAGCTGCTGGCCGTGGAGCTCCGCCTGGGAGGGGTGTACCACCAGTGAGGAGGTGA

Protein sequence

>MsG0480023399.01.T01

MSRSLGIPVKLLHEASGHVVTVELKSGELYRGNMIECEDNWNCQLESITYTAKDGKTSQLEHVFIRGSKVRFMVIPDMLKNAPMFKRLDARIKGKGASLGVGRGRAVAMRAKAQAAGRGAPPGRGVPPVRR*