Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022977.01.T01 | XP_012572296.1 | 88.732 | 71 | 7 | 1 | 1 | 71 | 25 | 94 | 1.19E-32 | 118 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022977.01.T01 | A0A1S3EBE5 | 88.732 | 71 | 7 | 1 | 1 | 71 | 25 | 94 | 5.71e-33 | 118 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000984.01 | MsG0480022977.01 | 0.833481 | 5.203959e-56 | 6.000452e-53 |
| MsG0180005678.01 | MsG0480022977.01 | 0.878951 | 1.852692e-69 | 9.860882e-66 |
| MsG0480022977.01 | MsG0580029739.01 | 0.854532 | 1.156117e-61 | 2.590133e-58 |
| MsG0280006924.01 | MsG0480022977.01 | 0.878924 | 1.893016e-69 | 1.006511e-65 |
| MsG0280008751.01 | MsG0480022977.01 | 0.804272 | 2.339213e-49 | 1.200946e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022977.01.T01 | MTR_8g097030 | 94.366 | 71 | 3 | 1 | 1 | 71 | 1 | 70 | 5.86e-29 | 98.2 |
| MsG0480022977.01.T01 | MTR_3g085020 | 71.014 | 69 | 19 | 1 | 3 | 71 | 2 | 69 | 1.77e-28 | 97.1 |
| MsG0480022977.01.T01 | MTR_8g096840 | 66.667 | 69 | 22 | 1 | 3 | 71 | 2 | 69 | 1.39e-27 | 94.7 |
| MsG0480022977.01.T01 | MTR_7g028975 | 63.768 | 69 | 24 | 1 | 3 | 71 | 2 | 69 | 2.26e-25 | 89.4 |
| MsG0480022977.01.T01 | MTR_8g027820 | 66.102 | 59 | 20 | 0 | 3 | 61 | 2 | 60 | 3.18e-23 | 85.1 |
| MsG0480022977.01.T01 | MTR_7g028870 | 64.615 | 65 | 22 | 1 | 1 | 65 | 1 | 64 | 5.47e-23 | 83.2 |
| MsG0480022977.01.T01 | MTR_8g097020 | 62.712 | 59 | 22 | 0 | 3 | 61 | 2 | 60 | 7.01e-23 | 82.8 |
| MsG0480022977.01.T01 | MTR_7g028815 | 64.815 | 54 | 19 | 0 | 3 | 56 | 2 | 55 | 7.14e-21 | 79.3 |
| MsG0480022977.01.T01 | MTR_3g085030 | 51.471 | 68 | 32 | 1 | 4 | 71 | 3 | 69 | 1.32e-19 | 74.7 |
| MsG0480022977.01.T01 | MTR_7g028940 | 55.932 | 59 | 26 | 0 | 3 | 61 | 2 | 60 | 1.46e-19 | 74.7 |
| MsG0480022977.01.T01 | MTR_7g028945 | 46.939 | 49 | 26 | 0 | 4 | 52 | 3 | 51 | 8.51e-12 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022977.01.T01 | AT3G48660 | 73.913 | 69 | 17 | 1 | 3 | 71 | 2 | 69 | 3.46e-29 | 99.4 |
| MsG0480022977.01.T01 | AT5G63500 | 71.014 | 69 | 19 | 1 | 3 | 71 | 2 | 69 | 1.17e-27 | 95.1 |
| MsG0480022977.01.T01 | AT5G40980 | 68.421 | 57 | 18 | 0 | 1 | 57 | 1 | 57 | 6.95e-24 | 85.5 |
| MsG0480022977.01.T01 | AT3G27027 | 62.295 | 61 | 23 | 0 | 1 | 61 | 1 | 61 | 6.30e-23 | 83.2 |
| MsG0480022977.01.T01 | AT3G27030 | 67.241 | 58 | 19 | 0 | 4 | 61 | 3 | 60 | 1.34e-21 | 82.0 |
| MsG0480022977.01.T01 | AT3G27030 | 57.971 | 69 | 28 | 1 | 3 | 71 | 75 | 142 | 1.17e-19 | 77.0 |
| MsG0480022977.01.T01 | AT5G08391 | 55.932 | 59 | 26 | 0 | 3 | 61 | 2 | 60 | 5.65e-21 | 78.2 |
| MsG0480022977.01.T01 | AT3G01950 | 55.738 | 61 | 27 | 0 | 1 | 61 | 1 | 61 | 6.47e-20 | 75.5 |
| MsG0480022977.01.T01 | AT5G14110 | 55.172 | 58 | 26 | 0 | 1 | 58 | 1 | 58 | 4.70e-19 | 73.2 |
| MsG0480022977.01.T01 | AT3G01940 | 66.102 | 59 | 20 | 0 | 3 | 61 | 4 | 62 | 8.37e-17 | 67.8 |
| MsG0480022977.01.T01 | AT5G50560 | 61.404 | 57 | 22 | 0 | 3 | 59 | 2 | 58 | 1.25e-14 | 62.0 |
| MsG0480022977.01.T01 | AT5G50660 | 61.404 | 57 | 22 | 0 | 3 | 59 | 2 | 58 | 1.25e-14 | 62.0 |
Find 30 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGGGAGGGTGGTAGCTTT+TGG | 0.170404 | 4:+79315633 | MsG0480022977.01.T01:CDS |
| AACAAGTGGTCTCTCTATTT+TGG | 0.192974 | 4:+79315665 | MsG0480022977.01.T01:CDS |
| CAAAGTGATGGCTGCTGATT+GGG | 0.288404 | 4:+79315542 | None:intergenic |
| TTTGATTATCCAGAGTAGTA+TGG | 0.356261 | 4:-79315747 | None:intergenic |
| GTCCATACCATCATCTTCTT+CGG | 0.384903 | 4:+79315687 | MsG0480022977.01.T01:CDS |
| ACTGTTCCAGCTCCCGGCTA+AGG | 0.387885 | 4:+79315614 | MsG0480022977.01.T01:CDS |
| TGCTGATTGGGCTCCGGTGG+TGG | 0.391799 | 4:+79315554 | MsG0480022977.01.T01:CDS |
| GCAAAGTGATGGCTGCTGAT+TGG | 0.392576 | 4:+79315541 | None:intergenic |
| CCCTCCCCTTAGCCGGGAGC+TGG | 0.420899 | 4:-79315620 | None:intergenic |
| CTGTTCCAGCTCCCGGCTAA+GGG | 0.432627 | 4:+79315615 | MsG0480022977.01.T01:CDS |
| CTGTTTGTGCTACTAAGTCC+AGG | 0.452174 | 4:+79315588 | MsG0480022977.01.T01:CDS |
| TTTCTACTTGATGCCATTGG+TGG | 0.461814 | 4:+79315723 | MsG0480022977.01.T01:CDS |
| CCAGCTCCCGGCTAAGGGGA+GGG | 0.482266 | 4:+79315620 | MsG0480022977.01.T01:CDS |
| GCTACCACCCTCCCCTTAGC+CGG | 0.484903 | 4:-79315627 | None:intergenic |
| CTACCACCCTCCCCTTAGCC+GGG | 0.486618 | 4:-79315626 | None:intergenic |
| AGGGCTACTGTTCCAGCTCC+CGG | 0.497556 | 4:+79315608 | MsG0480022977.01.T01:CDS |
| GGCTGCTGATTGGGCTCCGG+TGG | 0.498987 | 4:+79315551 | MsG0480022977.01.T01:CDS |
| TCCAGCTCCCGGCTAAGGGG+AGG | 0.518990 | 4:+79315619 | MsG0480022977.01.T01:CDS |
| GTCCGAAGAAGATGATGGTA+TGG | 0.522758 | 4:-79315689 | None:intergenic |
| GATGGCTGCTGATTGGGCTC+CGG | 0.524505 | 4:+79315548 | None:intergenic |
| GGGAGCTGGAACAGTAGCCC+TGG | 0.543144 | 4:-79315606 | None:intergenic |
| ATTGGTGGTCCATACTACTC+TGG | 0.565562 | 4:+79315738 | MsG0480022977.01.T01:CDS |
| GGCTCCGGTGGTGGTATCTG+TGG | 0.570677 | 4:+79315563 | MsG0480022977.01.T01:CDS |
| GCTCCCGGCTAAGGGGAGGG+TGG | 0.576467 | 4:+79315623 | MsG0480022977.01.T01:CDS |
| TGTTCCAGCTCCCGGCTAAG+GGG | 0.589394 | 4:+79315616 | MsG0480022977.01.T01:CDS |
| AACGAGTCCGAAGAAGATGA+TGG | 0.602203 | 4:-79315694 | None:intergenic |
| AGCACCACAGATACCACCAC+CGG | 0.611357 | 4:-79315567 | None:intergenic |
| AGAGTAGTATGGACCACCAA+TGG | 0.613237 | 4:-79315736 | None:intergenic |
| TTTGGGAGCATGCAAACAAG+TGG | 0.621437 | 4:+79315651 | MsG0480022977.01.T01:CDS |
| TGTTTGTGCTACTAAGTCCA+GGG | 0.634532 | 4:+79315589 | MsG0480022977.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGGCATCAAGTAGAAAAA+TGG | - | Chr4:79315721-79315740 | None:intergenic | 30.0% | |
| ! | ATTTTTCTACTTGATGCCAT+TGG | + | Chr4:79315720-79315739 | MsG0480022977.01.T01:CDS | 30.0% |
| ! | AACAAGTGGTCTCTCTATTT+TGG | + | Chr4:79315665-79315684 | MsG0480022977.01.T01:CDS | 35.0% |
| GTCCATACCATCATCTTCTT+CGG | + | Chr4:79315687-79315706 | MsG0480022977.01.T01:CDS | 40.0% | |
| TTTCTACTTGATGCCATTGG+TGG | + | Chr4:79315723-79315742 | MsG0480022977.01.T01:CDS | 40.0% | |
| ! | TGTTTGTGCTACTAAGTCCA+GGG | + | Chr4:79315589-79315608 | MsG0480022977.01.T01:CDS | 40.0% |
| AACGAGTCCGAAGAAGATGA+TGG | - | Chr4:79315697-79315716 | None:intergenic | 45.0% | |
| AGAGTAGTATGGACCACCAA+TGG | - | Chr4:79315739-79315758 | None:intergenic | 45.0% | |
| ! | ATTGGTGGTCCATACTACTC+TGG | + | Chr4:79315738-79315757 | MsG0480022977.01.T01:CDS | 45.0% |
| ! | CTGTTTGTGCTACTAAGTCC+AGG | + | Chr4:79315588-79315607 | MsG0480022977.01.T01:CDS | 45.0% |
| ! | GTCCGAAGAAGATGATGGTA+TGG | - | Chr4:79315692-79315711 | None:intergenic | 45.0% |
| ! | TTTGGGAGCATGCAAACAAG+TGG | + | Chr4:79315651-79315670 | MsG0480022977.01.T01:CDS | 45.0% |
| AGCACCACAGATACCACCAC+CGG | - | Chr4:79315570-79315589 | None:intergenic | 55.0% | |
| ! | AAGGGGAGGGTGGTAGCTTT+TGG | + | Chr4:79315633-79315652 | MsG0480022977.01.T01:CDS | 55.0% |
| ! | AGGGGAGGGTGGTAGCTTTT+GGG | + | Chr4:79315634-79315653 | MsG0480022977.01.T01:CDS | 55.0% |
| ACTGTTCCAGCTCCCGGCTA+AGG | + | Chr4:79315614-79315633 | MsG0480022977.01.T01:CDS | 60.0% | |
| AGGGCTACTGTTCCAGCTCC+CGG | + | Chr4:79315608-79315627 | MsG0480022977.01.T01:CDS | 60.0% | |
| CTGTTCCAGCTCCCGGCTAA+GGG | + | Chr4:79315615-79315634 | MsG0480022977.01.T01:CDS | 60.0% | |
| TGTTCCAGCTCCCGGCTAAG+GGG | + | Chr4:79315616-79315635 | MsG0480022977.01.T01:CDS | 60.0% | |
| CTACCACCCTCCCCTTAGCC+GGG | - | Chr4:79315629-79315648 | None:intergenic | 65.0% | |
| GCTACCACCCTCCCCTTAGC+CGG | - | Chr4:79315630-79315649 | None:intergenic | 65.0% | |
| GGGAGCTGGAACAGTAGCCC+TGG | - | Chr4:79315609-79315628 | None:intergenic | 65.0% | |
| ! | GGCTCCGGTGGTGGTATCTG+TGG | + | Chr4:79315563-79315582 | MsG0480022977.01.T01:CDS | 65.0% |
| ! | TGCTGATTGGGCTCCGGTGG+TGG | + | Chr4:79315554-79315573 | MsG0480022977.01.T01:CDS | 65.0% |
| CCAGCTCCCGGCTAAGGGGA+GGG | + | Chr4:79315620-79315639 | MsG0480022977.01.T01:CDS | 70.0% | |
| TCCAGCTCCCGGCTAAGGGG+AGG | + | Chr4:79315619-79315638 | MsG0480022977.01.T01:CDS | 70.0% | |
| ! | GGCTGCTGATTGGGCTCCGG+TGG | + | Chr4:79315551-79315570 | MsG0480022977.01.T01:CDS | 70.0% |
| CCCTCCCCTTAGCCGGGAGC+TGG | - | Chr4:79315623-79315642 | None:intergenic | 75.0% | |
| GCTCCCGGCTAAGGGGAGGG+TGG | + | Chr4:79315623-79315642 | MsG0480022977.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 79315549 | 79315764 | 79315549 | ID=MsG0480022977.01;Name=MsG0480022977.01 |
| Chr4 | mRNA | 79315549 | 79315764 | 79315549 | ID=MsG0480022977.01.T01;Parent=MsG0480022977.01;Name=MsG0480022977.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|0|1|0|71 |
| Chr4 | exon | 79315549 | 79315764 | 79315549 | ID=MsG0480022977.01.T01:exon:6079;Parent=MsG0480022977.01.T01 |
| Chr4 | CDS | 79315549 | 79315764 | 79315549 | ID=MsG0480022977.01.T01:cds;Parent=MsG0480022977.01.T01 |
| Gene Sequence |
| Protein sequence |
