AlfalfaGEDB | MsG0280006924.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006924.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006924.01.T01	XP_013462600.1	95	160	8	0	1	160	1	160	3.37E-88	265

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006924.01.T01	A0A072VEM8	95.000	160	8	0	1	160	1	160	1.61e-88	265

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000161.01	MsG0280006924.01	0.822166	2.739445e-53	2.275099e-50
MsG0180000984.01	MsG0280006924.01	0.930359	1.836269e-93	1.082600e-88
MsG0180002281.01	MsG0280006924.01	0.841503	4.567027e-58	6.724488e-55
MsG0180003863.01	MsG0280006924.01	0.813469	2.519914e-51	1.647066e-48
MsG0180003905.01	MsG0280006924.01	0.807591	4.690972e-50	2.623037e-47
MsG0180004098.01	MsG0280006924.01	0.816702	4.825150e-52	3.443722e-49
MsG0180004102.01	MsG0280006924.01	0.833460	5.266189e-56	6.068470e-53
MsG0180004120.01	MsG0280006924.01	0.832887	7.313203e-56	8.284344e-53
MsG0180004828.01	MsG0280006924.01	0.815452	9.176356e-52	6.329329e-49
MsG0180005621.01	MsG0280006924.01	0.825632	4.216649e-54	3.864793e-51
MsG0180005633.01	MsG0280006924.01	0.814760	1.307235e-51	8.847683e-49
MsG0180005678.01	MsG0280006924.01	0.922608	7.884178e-89	3.009478e-84
MsG0180005819.01	MsG0280006924.01	0.817271	3.595526e-52	2.606398e-49
MsG0180005924.01	MsG0280006924.01	0.823377	1.431063e-53	1.229961e-50
MsG0180006183.01	MsG0280006924.01	0.814216	1.724644e-51	1.150238e-48
MsG0280006909.01	MsG0280006924.01	0.812727	3.665329e-51	2.348314e-48
MsG0280006924.01	MsG0280007092.01	0.825301	5.051808e-54	4.586716e-51
MsG0280006924.01	MsG0280007695.01	0.803836	2.882620e-49	1.463503e-46
MsG0280006924.01	MsG0280008751.01	0.881141	3.070112e-70	1.777989e-66
MsG0280006924.01	MsG0280009980.01	0.832985	6.915400e-56	7.857269e-53
MsG0280006924.01	MsG0280010825.01	0.809555	1.785804e-50	1.051777e-47
MsG0280006924.01	MsG0380014542.01	0.804450	2.147586e-49	1.107625e-46
MsG0280006924.01	MsG0380014544.01	0.851297	9.729746e-61	1.960511e-57
MsG0280006924.01	MsG0380015340.01	0.803256	3.802847e-49	1.902071e-46
MsG0280006924.01	MsG0380015656.01	0.828387	9.247894e-55	9.178786e-52
MsG0280006924.01	MsG0380016031.01	0.818084	2.357197e-52	1.747203e-49
MsG0280006924.01	MsG0380016819.01	0.805266	1.450125e-49	7.637852e-47
MsG0280006924.01	MsG0380016875.01	0.836457	9.252593e-57	1.166732e-53
MsG0280006924.01	MsG0380017421.01	0.839468	1.556053e-57	2.150858e-54
MsG0280006924.01	MsG0380017696.01	0.812385	4.353980e-51	2.764448e-48
MsG0280006924.01	MsG0480018228.01	0.830233	3.295434e-55	3.451722e-52
MsG0280006924.01	MsG0480018362.01	0.802629	5.124654e-49	2.522508e-46
MsG0280006924.01	MsG0480020366.01	0.815900	7.294593e-52	5.093011e-49
MsG0280006924.01	MsG0480020398.01	0.807389	5.176117e-50	2.879433e-47
MsG0280006924.01	MsG0480020937.01	0.867653	1.167849e-65	4.108231e-62
MsG0280006924.01	MsG0480021486.01	0.818119	2.314308e-52	1.717199e-49
MsG0280006924.01	MsG0480021740.01	0.802139	6.463396e-49	3.142047e-46
MsG0280006924.01	MsG0480021773.01	0.832398	9.670464e-56	1.079638e-52
MsG0280006924.01	MsG0480021902.01	0.817000	4.137633e-52	2.977525e-49
MsG0280006924.01	MsG0480022307.01	0.812755	3.613424e-51	2.316719e-48
MsG0280006924.01	MsG0480022378.01	0.837398	5.322175e-57	6.904426e-54
MsG0280006924.01	MsG0480022977.01	0.878924	1.893016e-69	1.006511e-65
MsG0280006924.01	MsG0480023225.01	0.836113	1.131514e-56	1.412149e-53
MsG0280006924.01	MsG0480023414.01	0.806877	6.645577e-50	3.647896e-47
MsG0280006924.01	MsG0480023575.01	0.855095	7.939100e-62	1.811998e-58
MsG0280006924.01	MsG0480023626.01	0.843225	1.596659e-58	2.481273e-55
MsG0280006924.01	MsG0480023913.01	0.824379	8.335171e-54	7.370214e-51
MsG0280006924.01	MsG0480024019.01	0.842730	2.162787e-58	3.308965e-55
MsG0280006924.01	MsG0580024276.01	0.853627	2.108404e-61	4.584538e-58
MsG0280006924.01	MsG0580025504.01	0.800741	1.250290e-48	5.866538e-46
MsG0280006924.01	MsG0580025970.01	0.845081	5.072533e-59	8.357341e-56
MsG0280006924.01	MsG0580026343.01	0.809943	1.473753e-50	8.769209e-48
MsG0280006924.01	MsG0580028098.01	0.804753	1.856803e-49	9.652503e-47
MsG0280006924.01	MsG0580028884.01	0.871408	6.992111e-67	2.817699e-63
MsG0280006924.01	MsG0580028916.01	0.819325	1.232314e-52	9.452554e-50
MsG0280006924.01	MsG0580028955.01	0.809678	1.680719e-50	9.931354e-48
MsG0280006924.01	MsG0580029739.01	0.893632	5.244206e-75	5.037665e-71
MsG0280006924.01	MsG0580030141.01	0.840512	8.316421e-58	1.187380e-54
MsG0280006924.01	MsG0680031356.01	0.827681	1.367599e-54	1.329611e-51
MsG0280006924.01	MsG0680033645.01	0.808940	2.419399e-50	1.401645e-47
MsG0280006924.01	MsG0680035792.01	0.808123	3.614993e-50	2.049903e-47
MsG0280006924.01	MsG0780036901.01	0.812260	4.636251e-51	2.933816e-48
MsG0280006924.01	MsG0780037371.01	0.808798	2.594250e-50	1.497309e-47
MsG0280006924.01	MsG0780038647.01	0.841719	4.007185e-58	5.939062e-55
MsG0280006924.01	MsG0780038736.01	0.800390	1.474135e-48	6.855629e-46
MsG0280006924.01	MsG0780038952.01	0.832059	1.173316e-55	1.296855e-52
MsG0280006924.01	MsG0780039536.01	0.805331	1.405332e-49	7.414510e-47
MsG0280006924.01	MsG0780040459.01	0.838510	2.755226e-57	3.698476e-54
MsG0280006924.01	MsG0780040554.01	0.829975	3.808928e-55	3.959749e-52
MsG0280006924.01	MsG0880042098.01	0.803428	3.502738e-49	1.759756e-46
MsG0280006924.01	MsG0880043920.01	0.919583	3.755528e-87	1.214264e-82
MsG0280006924.01	MsG0880045090.01	0.862470	4.955850e-64	1.453073e-60
MsG0280006924.01	MsG0880045879.01	0.818009	2.450889e-52	1.812993e-49
MsG0280006924.01	MsG0880046319.01	0.802300	5.989367e-49	2.923739e-46
MsG0280006924.01	MsG0880046940.01	0.836656	8.235333e-57	1.044518e-53
MsG0280006924.01	MsG0880047080.01	0.833552	4.994435e-56	5.771556e-53
MsG0280006924.01	MsG0880047228.01	0.875034	4.233874e-68	1.948473e-64
MsG0280006924.01	MsG0880047414.01	0.807585	4.703752e-50	2.629802e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006924.01.T01	MTR_2g016680	95.000	160	8	0	1	160	1	160	4.09e-92	265

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006924.01.T01	AT1G63310	45.902	122	57	4	14	130	8	125	3.56e-27	100

Find 45 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATGTAAACAGCAACTTTC+AGG	0.235009	2:+8024502	MsG0280006924.01.T01:CDS
TGCCATGCTTCTCAGCCTTA+TGG	0.243820	2:-8024622	None:intergenic
TTAATCAGAATGTTCACTTT+TGG	0.281937	2:-8024684	None:intergenic
TTGTGCTTCTCACCTTCAGC+AGG	0.342861	2:-8024260	None:intergenic
ACATAAGTGCTCAATGCTTC+AGG	0.349738	2:-8024485	None:intergenic
TCCTTCGTTGTTTCCTGCTA+GGG	0.401287	2:-8024405	None:intergenic
CTCCTTCGTTGTTTCCTGCT+AGG	0.406740	2:-8024406	None:intergenic
ACCAGGCTCCACCCACCATT+TGG	0.415263	2:+8024349	MsG0280006924.01.T01:CDS
CATGTGCACACCAGGATCAT+TGG	0.419356	2:-8024564	None:intergenic
AGCTACTCTGCGGAGCGAAT+TGG	0.426532	2:+8024427	MsG0280006924.01.T01:CDS
AGAACCTGGATAGAGAAATC+AGG	0.435585	2:+8024282	MsG0280006924.01.T01:CDS
AGCGAATTGGATGCAAAATC+AGG	0.436872	2:+8024440	MsG0280006924.01.T01:CDS
TCTCCCTGATTTCTCTATCC+AGG	0.452188	2:-8024286	None:intergenic
TCCAAATGGTGGGTGGAGCC+TGG	0.470522	2:-8024350	None:intergenic
GTTCATCGTCATTGTCCAAA+TGG	0.475721	2:-8024364	None:intergenic
GACAAGAAGAAAGGGAAAGA+AGG	0.488036	2:+8024659	MsG0280006924.01.T01:CDS
CATCAACAATTCCATCATGC+TGG	0.490788	2:+8024526	MsG0280006924.01.T01:CDS
ACTAGGATCATCACCCTAGC+AGG	0.495169	2:+8024392	MsG0280006924.01.T01:CDS
GCCTCCTCAAAATCACCATA+AGG	0.504627	2:+8024607	MsG0280006924.01.T01:CDS
CAGCATGATGGAATTGTTGA+TGG	0.507289	2:-8024525	None:intergenic
TGTGCTTCTCACCTTCAGCA+GGG	0.509920	2:-8024259	None:intergenic
AGGTGAGAAGCACAAGAACC+TGG	0.512517	2:+8024268	MsG0280006924.01.T01:CDS
TCAATGCTTCAGGCTCATCA+TGG	0.519187	2:-8024475	None:intergenic
AGTTACCATGCCAATGATCC+TGG	0.520944	2:+8024554	MsG0280006924.01.T01:CDS
TGCTTCTCACCTTCAGCAGG+GGG	0.521846	2:-8024257	None:intergenic
GTGCTTCTCACCTTCAGCAG+GGG	0.527227	2:-8024258	None:intergenic
GGCTGAGAAGCATGGCAAGA+AGG	0.528484	2:+8024628	MsG0280006924.01.T01:CDS
GAAATATCCATGTGCACACC+AGG	0.542565	2:-8024572	None:intergenic
ATCAACAATTCCATCATGCT+GGG	0.549338	2:+8024527	MsG0280006924.01.T01:CDS
CACCATAAGGCTGAGAAGCA+TGG	0.558365	2:+8024620	MsG0280006924.01.T01:CDS
ATCGTCATTGTCCAAATGGT+GGG	0.558386	2:-8024360	None:intergenic
GAACCTGGATAGAGAAATCA+GGG	0.559810	2:+8024283	MsG0280006924.01.T01:CDS
CAATGCTTCAGGCTCATCAT+GGG	0.565922	2:-8024474	None:intergenic
ATCAGTGACACGATGAGTGA+TGG	0.571133	2:-8024318	None:intergenic
CATCGTCATTGTCCAAATGG+TGG	0.582124	2:-8024361	None:intergenic
GGATAGAGAAATCAGGGAGA+TGG	0.584444	2:+8024289	MsG0280006924.01.T01:CDS
GTAACTACCACCCAGCATGA+TGG	0.598915	2:-8024537	None:intergenic
CAATGATCCTGGTGTGCACA+TGG	0.602886	2:+8024565	MsG0280006924.01.T01:CDS
TTGGACAATGACGATGAACA+TGG	0.605622	2:+8024368	MsG0280006924.01.T01:CDS
ATGACGATGAACATGGCACT+AGG	0.616583	2:+8024375	MsG0280006924.01.T01:CDS
GTCATTGTCCAAATGGTGGG+TGG	0.618594	2:-8024357	None:intergenic
GCACACCAGGATCATTGGCA+TGG	0.654233	2:-8024559	None:intergenic
AACAATTCCATCATGCTGGG+TGG	0.675369	2:+8024530	MsG0280006924.01.T01:CDS
ACCCTAGCAGGAAACAACGA+AGG	0.700828	2:+8024404	MsG0280006924.01.T01:CDS
ACAACGAAGGAGCTACTCTG+CGG	0.747013	2:+8024417	MsG0280006924.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAAAAGGACAAGAAGAAA+GGG	+	Chr2:8024651-8024670	MsG0280006924.01.T01:CDS	25.0%
!!	TTAATCAGAATGTTCACTTT+TGG	-	Chr2:8024687-8024706	None:intergenic	25.0%
	GAAAAAAAGGACAAGAAGAA+AGG	+	Chr2:8024650-8024669	MsG0280006924.01.T01:CDS	30.0%
	TTATGTAAACAGCAACTTTC+AGG	+	Chr2:8024502-8024521	MsG0280006924.01.T01:CDS	30.0%
	ATCAACAATTCCATCATGCT+GGG	+	Chr2:8024527-8024546	MsG0280006924.01.T01:CDS	35.0%
!!!	GTCACTGATTTTCATAAACC+AGG	+	Chr2:8024332-8024351	MsG0280006924.01.T01:CDS	35.0%
	ACATAAGTGCTCAATGCTTC+AGG	-	Chr2:8024488-8024507	None:intergenic	40.0%
	AGAACCTGGATAGAGAAATC+AGG	+	Chr2:8024282-8024301	MsG0280006924.01.T01:CDS	40.0%
	AGCGAATTGGATGCAAAATC+AGG	+	Chr2:8024440-8024459	MsG0280006924.01.T01:CDS	40.0%
	ATCGTCATTGTCCAAATGGT+GGG	-	Chr2:8024363-8024382	None:intergenic	40.0%
	CATCAACAATTCCATCATGC+TGG	+	Chr2:8024526-8024545	MsG0280006924.01.T01:CDS	40.0%
	GAACCTGGATAGAGAAATCA+GGG	+	Chr2:8024283-8024302	MsG0280006924.01.T01:CDS	40.0%
	GACAAGAAGAAAGGGAAAGA+AGG	+	Chr2:8024659-8024678	MsG0280006924.01.T01:CDS	40.0%
	GCATGGCAAGAAGGAAAAAA+AGG	+	Chr2:8024637-8024656	MsG0280006924.01.T01:CDS	40.0%
	GTTCATCGTCATTGTCCAAA+TGG	-	Chr2:8024367-8024386	None:intergenic	40.0%
	TTGGACAATGACGATGAACA+TGG	+	Chr2:8024368-8024387	MsG0280006924.01.T01:CDS	40.0%
!	CAGCATGATGGAATTGTTGA+TGG	-	Chr2:8024528-8024547	None:intergenic	40.0%
!!	TCAGCCTTATGGTGATTTTG+AGG	-	Chr2:8024614-8024633	None:intergenic	40.0%
	AACAATTCCATCATGCTGGG+TGG	+	Chr2:8024530-8024549	MsG0280006924.01.T01:CDS	45.0%
	AGTTACCATGCCAATGATCC+TGG	+	Chr2:8024554-8024573	MsG0280006924.01.T01:CDS	45.0%
	ATCAGTGACACGATGAGTGA+TGG	-	Chr2:8024321-8024340	None:intergenic	45.0%
	ATGACGATGAACATGGCACT+AGG	+	Chr2:8024375-8024394	MsG0280006924.01.T01:CDS	45.0%
	CAATGCTTCAGGCTCATCAT+GGG	-	Chr2:8024477-8024496	None:intergenic	45.0%
	CATCGTCATTGTCCAAATGG+TGG	-	Chr2:8024364-8024383	None:intergenic	45.0%
	GAAATATCCATGTGCACACC+AGG	-	Chr2:8024575-8024594	None:intergenic	45.0%
	GCCTCCTCAAAATCACCATA+AGG	+	Chr2:8024607-8024626	MsG0280006924.01.T01:CDS	45.0%
	GGATAGAGAAATCAGGGAGA+TGG	+	Chr2:8024289-8024308	MsG0280006924.01.T01:CDS	45.0%
	TCAATGCTTCAGGCTCATCA+TGG	-	Chr2:8024478-8024497	None:intergenic	45.0%
	TCTCCCTGATTTCTCTATCC+AGG	-	Chr2:8024289-8024308	None:intergenic	45.0%
!	TCCTTCGTTGTTTCCTGCTA+GGG	-	Chr2:8024408-8024427	None:intergenic	45.0%
!!	ATGGTGATTTTGAGGAGGCT+CGG	-	Chr2:8024606-8024625	None:intergenic	45.0%
!!	GCCTTATGGTGATTTTGAGG+AGG	-	Chr2:8024611-8024630	None:intergenic	45.0%
	ACAACGAAGGAGCTACTCTG+CGG	+	Chr2:8024417-8024436	MsG0280006924.01.T01:CDS	50.0%
	ACCCTAGCAGGAAACAACGA+AGG	+	Chr2:8024404-8024423	MsG0280006924.01.T01:CDS	50.0%
	ACTAGGATCATCACCCTAGC+AGG	+	Chr2:8024392-8024411	MsG0280006924.01.T01:CDS	50.0%
	CAATGATCCTGGTGTGCACA+TGG	+	Chr2:8024565-8024584	MsG0280006924.01.T01:CDS	50.0%
	CACCATAAGGCTGAGAAGCA+TGG	+	Chr2:8024620-8024639	MsG0280006924.01.T01:CDS	50.0%
	CATGTGCACACCAGGATCAT+TGG	-	Chr2:8024567-8024586	None:intergenic	50.0%
	GTAACTACCACCCAGCATGA+TGG	-	Chr2:8024540-8024559	None:intergenic	50.0%
	TGCCATGCTTCTCAGCCTTA+TGG	-	Chr2:8024625-8024644	None:intergenic	50.0%
	TGTGCTTCTCACCTTCAGCA+GGG	-	Chr2:8024262-8024281	None:intergenic	50.0%
	TTGTGCTTCTCACCTTCAGC+AGG	-	Chr2:8024263-8024282	None:intergenic	50.0%
!	AGGTGAGAAGCACAAGAACC+TGG	+	Chr2:8024268-8024287	MsG0280006924.01.T01:CDS	50.0%
!	CTCCTTCGTTGTTTCCTGCT+AGG	-	Chr2:8024409-8024428	None:intergenic	50.0%
!	GTCATTGTCCAAATGGTGGG+TGG	-	Chr2:8024360-8024379	None:intergenic	50.0%
	AGCTACTCTGCGGAGCGAAT+TGG	+	Chr2:8024427-8024446	MsG0280006924.01.T01:CDS	55.0%
	GCACACCAGGATCATTGGCA+TGG	-	Chr2:8024562-8024581	None:intergenic	55.0%
	GTGCTTCTCACCTTCAGCAG+GGG	-	Chr2:8024261-8024280	None:intergenic	55.0%
	TGCTTCTCACCTTCAGCAGG+GGG	-	Chr2:8024260-8024279	None:intergenic	55.0%
!	GGCTGAGAAGCATGGCAAGA+AGG	+	Chr2:8024628-8024647	MsG0280006924.01.T01:CDS	55.0%
	ACCAGGCTCCACCCACCATT+TGG	+	Chr2:8024349-8024368	MsG0280006924.01.T01:CDS	60.0%
	AGTCACTCTCCCCCTGCTGA+AGG	+	Chr2:8024248-8024267	MsG0280006924.01.T01:CDS	60.0%
!!	TCCAAATGGTGGGTGGAGCC+TGG	-	Chr2:8024353-8024372	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	8024224	8024706	8024224	ID=MsG0280006924.01;Name=MsG0280006924.01
Chr2	mRNA	8024224	8024706	8024224	ID=MsG0280006924.01.T01;Parent=MsG0280006924.01;Name=MsG0280006924.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|160
Chr2	exon	8024224	8024706	8024224	ID=MsG0280006924.01.T01:exon:2318;Parent=MsG0280006924.01.T01
Chr2	CDS	8024224	8024706	8024224	ID=MsG0280006924.01.T01:cds;Parent=MsG0280006924.01.T01

Gene Sequence

>MsG0280006924.01.T01

ATGCATTCAGAGTCAGTGAAGTCAAGTCACTCTCCCCCTGCTGAAGGTGAGAAGCACAAGAACCTGGATAGAGAAATCAGGGAGATGGTATCTGCCATCACTCATCGTGTCACTGATTTTCATAAACCAGGCTCCACCCACCATTTGGACAATGACGATGAACATGGCACTAGGATCATCACCCTAGCAGGAAACAACGAAGGAGCTACTCTGCGGAGCGAATTGGATGCAAAATCAGGTAAATATTCTTCCCATGATGAGCCTGAAGCATTGAGCACTTATGTAAACAGCAACTTTCAGGCCATCAACAATTCCATCATGCTGGGTGGTAGTTACCATGCCAATGATCCTGGTGTGCACATGGATATTTCAGATTTCACCGAGCCTCCTCAAAATCACCATAAGGCTGAGAAGCATGGCAAGAAGGAAAAAAAGGACAAGAAGAAAGGGAAAGAAGGTTCCAAAAGTGAACATTCTGATTAA

Protein sequence

>MsG0280006924.01.T01

MHSESVKSSHSPPAEGEKHKNLDREIREMVSAITHRVTDFHKPGSTHHLDNDDEHGTRIITLAGNNEGATLRSELDAKSGKYSSHDEPEALSTYVNSNFQAINNSIMLGGSYHANDPGVHMDISDFTEPPQNHHKAEKHGKKEKKDKKKGKEGSKSEHSD*