AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023339.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023339.01.T01 MTR_8g090375 96.067 356 10 1 1 352 43 398 0.0 709
MsG0480023339.01.T01 MTR_4g079290 43.910 312 149 5 59 351 95 399 2.28e-79 256
MsG0480023339.01.T01 MTR_5g012920 40.467 257 139 5 101 353 103 349 3.55e-54 189
MsG0480023339.01.T01 MTR_4g100640 40.157 254 138 5 101 350 103 346 4.41e-53 183
MsG0480023339.01.T01 MTR_4g100640 40.157 254 138 5 101 350 103 346 4.81e-52 183
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023339.01.T01 AT5G65110 82.022 356 59 2 2 352 47 402 0.0 613
MsG0480023339.01.T01 AT5G65110 82.022 356 59 2 2 352 47 402 0.0 613
MsG0480023339.01.T01 AT1G06290 44.660 309 155 6 51 351 98 398 1.32e-78 254
MsG0480023339.01.T01 AT1G06310 43.042 309 160 6 51 351 98 398 4.56e-77 250
MsG0480023339.01.T01 AT4G16760 40.157 254 138 5 101 350 90 333 4.67e-53 186
MsG0480023339.01.T01 AT4G16760 40.157 254 138 5 101 350 103 346 6.17e-53 185
MsG0480023339.01.T01 AT2G35690 39.147 258 143 5 97 350 99 346 3.79e-52 183

Find 99 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTGGTTCTGTAATCAATT+TGG 0.224713 4:+83998767 MsG0480023339.01.T01:CDS
CAATTCTCGGTGATTATCTT+TGG 0.226333 4:-83998501 None:intergenic
ATGCTTTCATTGTTCCAATA+AGG 0.244174 4:+83999875 MsG0480023339.01.T01:CDS
TCTCTACTTCGTCAGCAATT+TGG 0.245338 4:+84000192 MsG0480023339.01.T01:CDS
GTTTATTCTATGCAGTCTTT+GGG 0.259624 4:+83998746 MsG0480023339.01.T01:intron
TGGGTCTTCATATCCCTTAT+TGG 0.284324 4:-83999889 None:intergenic
CATCTCCAAAACGGTTTAGA+AGG 0.308919 4:-84000020 None:intergenic
TGCTTTCATTGTTCCAATAA+GGG 0.312257 4:+83999876 MsG0480023339.01.T01:CDS
CTATGATGGTATTGATAACT+TGG 0.333947 4:+83998815 MsG0480023339.01.T01:CDS
GACAACCTTCTAAACCGTTT+TGG 0.334424 4:+84000015 MsG0480023339.01.T01:CDS
TATCTTTGGAAATTTCAATA+GGG 0.356130 4:-83998487 None:intergenic
GGTGGTTCTGTAATCAATTT+GGG 0.359274 4:+83998768 MsG0480023339.01.T01:CDS
TGTAAGCGTCCTCAAGGTTT+CGG 0.365459 4:+84000149 MsG0480023339.01.T01:CDS
AAGACCCATCAAACACTTCC+TGG 0.370749 4:+83999904 MsG0480023339.01.T01:CDS
ATTGTGTATGAATCAGCTTT+TGG 0.372478 4:+83998520 MsG0480023339.01.T01:CDS
TTATCTTTGGAAATTTCAAT+AGG 0.377781 4:-83998488 None:intergenic
TTGGCTCTTGTTAGAGAAGC+TGG 0.387447 4:+83998539 MsG0480023339.01.T01:CDS
AGCGGTTCGGTGCAACTCTC+GGG 0.390305 4:+84000088 MsG0480023339.01.T01:CDS
GCTGATTCATACACAATTCT+CGG 0.398942 4:-83998514 None:intergenic
CATTTGATAGCACCATCATT+TGG 0.402058 4:-83999751 None:intergenic
GGCCTCAATGGTGTTGATAA+TGG 0.420530 4:+83999958 MsG0480023339.01.T01:CDS
CAATTCCAGGAAGTGTTTGA+TGG 0.423634 4:-83999909 None:intergenic
ATTATTGATACTCCAAATGA+TGG 0.432472 4:+83999739 MsG0480023339.01.T01:CDS
CAATCATGTATCTCAATTCC+AGG 0.438247 4:-83999922 None:intergenic
TTTGGAGATGTTTCTCGTGA+TGG 0.445538 4:+84000033 MsG0480023339.01.T01:CDS
CATAAAAGGTTCAAATGTGC+AGG 0.449075 4:+83999672 MsG0480023339.01.T01:intron
AAGCGGTTCGGTGCAACTCT+CGG 0.453349 4:+84000087 MsG0480023339.01.T01:CDS
CCTTCGGTTAATAAGCGGTT+CGG 0.458831 4:+84000075 MsG0480023339.01.T01:CDS
TGCAAAAGGAGTTTCTGATA+TGG 0.461080 4:+83999846 MsG0480023339.01.T01:CDS
GCAACTCTCGGGGAACTTGT+TGG 0.464445 4:+84000099 MsG0480023339.01.T01:CDS
CGGGGAACTTGTTGGTGGTA+GGG 0.466679 4:+84000107 MsG0480023339.01.T01:CDS
TAGTCGAGAATCGTTACTTC+GGG 0.467616 4:-84000228 None:intergenic
TCCTCTCATGTAACCGGAGA+GGG 0.480165 4:-83998402 None:intergenic
TATGTCGCTTGGAATCAAAA+TGG 0.490363 4:+83998636 MsG0480023339.01.T01:CDS
TTACCTAATTATGATGCAAA+AGG 0.492396 4:+83999832 MsG0480023339.01.T01:CDS
TTGGAGATGTTTCTCGTGAT+GGG 0.492414 4:+84000034 MsG0480023339.01.T01:CDS
GTACACAAGTACGCTTCCTT+CGG 0.492471 4:+84000059 MsG0480023339.01.T01:CDS
CATGATTGTGGTCATAAAGT+TGG 0.493032 4:+83999937 MsG0480023339.01.T01:CDS
TCGGGGAACTTGTTGGTGGT+AGG 0.493211 4:+84000106 MsG0480023339.01.T01:CDS
ATCTTTGGAAATTTCAATAG+GGG 0.494569 4:-83998486 None:intergenic
AAAATAGCAAAATACTTAGC+AGG 0.495694 4:-83998593 None:intergenic
GGTCATAAAGTTGGCCTCAA+TGG 0.500739 4:+83999946 MsG0480023339.01.T01:CDS
GTTACTTCGGGTTGGTCTCG+AGG 0.502846 4:-84000216 None:intergenic
TCAATAGGGGTTAGAAGATG+TGG 0.503081 4:-83998473 None:intergenic
TAAGCTTATGCTGATGAGAC+TGG 0.505882 4:-84000251 None:intergenic
AGAATATCTGATGGCAATAG+TGG 0.520586 4:-84000172 None:intergenic
CATAAGGACAAGTACTATGA+TGG 0.522129 4:+83998801 MsG0480023339.01.T01:CDS
GCATAAGCTTATGCCCATGC+TGG 0.522352 4:+84000263 MsG0480023339.01.T01:CDS
GAACTTGTTGGTGGTAGGGT+AGG 0.522927 4:+84000111 MsG0480023339.01.T01:CDS
TTTGGGAACACAGAAACATA+AGG 0.529122 4:+83998785 MsG0480023339.01.T01:CDS
TGCATAAGTTGAAGCCAGCA+TGG 0.533986 4:-84000277 None:intergenic
TTCTTCTGTAAGCGTCCTCA+AGG 0.534074 4:+84000143 MsG0480023339.01.T01:CDS
AATTCCAGGAAGTGTTTGAT+GGG 0.535427 4:-83999908 None:intergenic
CAAAGGTGGCGAGAGTTTGA+AGG 0.542136 4:-83999696 None:intergenic
GCAAAAGGAGTTTCTGATAT+GGG 0.548158 4:+83999847 MsG0480023339.01.T01:CDS
CACGAGAAACATCTCCAAAA+CGG 0.548477 4:-84000029 None:intergenic
ATTCTATGCAGTCTTTGGGG+TGG 0.550468 4:+83998750 MsG0480023339.01.T01:intron
ATAATAAATTCATCGGTGAT+CGG 0.551438 4:-83999721 None:intergenic
ATGTCGCTTGGAATCAAAAT+GGG 0.554551 4:+83998637 MsG0480023339.01.T01:CDS
GTAGTCGAGAATCGTTACTT+CGG 0.559361 4:-84000229 None:intergenic
TTCATCGGTGATCGGATCAA+AGG 0.561998 4:-83999713 None:intergenic
ATTGGCAATGCCGCCATGCA+TGG 0.563361 4:+83999778 MsG0480023339.01.T01:CDS
CGAGAATCGTTACTTCGGGT+TGG 0.565234 4:-84000224 None:intergenic
GGTGCTATCAAATGGTGGAT+TGG 0.567024 4:+83999760 MsG0480023339.01.T01:CDS
TTTATTCTATGCAGTCTTTG+GGG 0.568032 4:+83998747 MsG0480023339.01.T01:intron
CGCTTCCTTCGGTTAATAAG+CGG 0.569278 4:+84000070 MsG0480023339.01.T01:CDS
TCCCTCTCCGGTTACATGAG+AGG 0.572675 4:+83998401 MsG0480023339.01.T01:CDS
GTGGACACAAATTCCCTCTC+CGG 0.574120 4:+83998389 MsG0480023339.01.T01:CDS
TCGGTCATAGCAAAACAACC+TGG 0.575066 4:-83998843 None:intergenic
AGTATCAATAATAAATTCAT+CGG 0.575429 4:-83999728 None:intergenic
AGATGGAAAATTGCAGTCGC+GGG 0.576755 4:+83998354 None:intergenic
ATTTCTCCACTAAGTTTGTG+TGG 0.582615 4:-84000310 None:intergenic
CAAATGATGGTGCTATCAAA+TGG 0.582753 4:+83999752 MsG0480023339.01.T01:CDS
GAAATTACCATGGTGAAGCT+CGG 0.587206 4:-83998862 None:intergenic
ATTGATAACTTGGATTACCC+AGG 0.595258 4:+83998825 MsG0480023339.01.T01:CDS
TGGTAGTGTGATATGTCGCT+TGG 0.598904 4:+83998625 MsG0480023339.01.T01:CDS
CTCCATTATCAACACCATTG+AGG 0.603969 4:-83999960 None:intergenic
GCATAAGTTGAAGCCAGCAT+GGG 0.604077 4:-84000276 None:intergenic
CGCTTACAGAAGAATATGCA+AGG 0.604155 4:-84000134 None:intergenic
ACTCTCGGGGAACTTGTTGG+TGG 0.607843 4:+84000102 MsG0480023339.01.T01:CDS
ATCGGTGATCGGATCAAAGG+TGG 0.617655 4:-83999710 None:intergenic
TGTGGCAAACTTGCCATGCA+TGG 0.619619 4:-83999791 None:intergenic
GAGATGGAAAATTGCAGTCG+CGG 0.623955 4:+83998353 None:intergenic
TGGAGCTCTTAGATTCCGCT+CGG 0.628031 4:+83999978 MsG0480023339.01.T01:CDS
CAATAGTGGCCGAAACCTTG+AGG 0.628582 4:-84000158 None:intergenic
CGGTCATAGCAAAACAACCT+GGG 0.629589 4:-83998842 None:intergenic
TCATTAACAACATAATGAAG+AGG 0.630936 4:-83998569 None:intergenic
GCGGTTCGGTGCAACTCTCG+GGG 0.635090 4:+84000089 MsG0480023339.01.T01:CDS
CGGTTTAGAAGGTTGTCTCG+AGG 0.643754 4:-84000009 None:intergenic
GCTATGACCGAGCTTCACCA+TGG 0.647722 4:+83998855 MsG0480023339.01.T01:CDS
TTCCTCTCATGTAACCGGAG+AGG 0.655151 4:-83998403 None:intergenic
CCGAACCGCTTATTAACCGA+AGG 0.655393 4:-84000075 None:intergenic
ACGAAGTAGAGAATATCTGA+TGG 0.663385 4:-84000181 None:intergenic
ATAAAAGGTTCAAATGTGCA+GGG 0.666780 4:+83999673 MsG0480023339.01.T01:intron
TCGCGGGAAGAAATTAATCG+TGG 0.674882 4:+83998370 MsG0480023339.01.T01:CDS
CTCGAGGAATTCTCACCGAG+CGG 0.676358 4:-83999993 None:intergenic
ATGATGGTGCTATCAAATGG+TGG 0.677203 4:+83999755 MsG0480023339.01.T01:CDS
GGAATTGAGATACATGATTG+TGG 0.680398 4:+83999925 MsG0480023339.01.T01:CDS
GGCAAACTTGCCATGCATGG+CGG 0.690153 4:-83999788 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTTTTTTTTTTTCATTT+GGG + Chr4:83998674-83998693 MsG0480023339.01.T01:intron 10.0%
!!! TATGTTTTTTTTTTTTCATT+TGG + Chr4:83998673-83998692 MsG0480023339.01.T01:intron 10.0%
!!! TATTTGTTTTTATCTTAATT+GGG + Chr4:83999572-83999591 MsG0480023339.01.T01:intron 10.0%
!! AGTATCAATAATAAATTCAT+CGG - Chr4:83999731-83999750 None:intergenic 15.0%
!!! CTATTTGTTTTTATCTTAAT+TGG + Chr4:83999571-83999590 MsG0480023339.01.T01:intron 15.0%
!! TCGTGTTCATAAAAATATAA+AGG + Chr4:83999351-83999370 MsG0480023339.01.T01:intron 20.0%
!! TGTGAGATAAAATTCAAAAT+GGG + Chr4:83999477-83999496 MsG0480023339.01.T01:intron 20.0%
!! TTGTGAGATAAAATTCAAAA+TGG + Chr4:83999476-83999495 MsG0480023339.01.T01:intron 20.0%
!!! ATAAAAATATAAAGGCATGT+GGG + Chr4:83999359-83999378 MsG0480023339.01.T01:intron 20.0%
!!! TATCTTTGGAAATTTCAATA+GGG - Chr4:83998490-83998509 None:intergenic 20.0%
!!! TTATCTTTGGAAATTTCAAT+AGG - Chr4:83998491-83998510 None:intergenic 20.0%
!!! TTTTCTTTTTTGTGCATAAA+AGG + Chr4:83999658-83999677 MsG0480023339.01.T01:intron 20.0%
!!! TTTTGTTTTGTTTTGATGAA+TGG + Chr4:83998724-83998743 MsG0480023339.01.T01:intron 20.0%
! AAAATAGCAAAATACTTAGC+AGG - Chr4:83998596-83998615 None:intergenic 25.0%
! ATAATAAATTCATCGGTGAT+CGG - Chr4:83999724-83999743 None:intergenic 25.0%
! ATTATTGATACTCCAAATGA+TGG + Chr4:83999739-83999758 MsG0480023339.01.T01:CDS 25.0%
! ATTTGGTAGAGTAATTAAAG+CGG + Chr4:83999429-83999448 MsG0480023339.01.T01:intron 25.0%
! TATCTTAATTGGGTTTCTAT+TGG + Chr4:83999582-83999601 MsG0480023339.01.T01:intron 25.0%
! TCATTAACAACATAATGAAG+AGG - Chr4:83998572-83998591 None:intergenic 25.0%
! TTACCTAATTATGATGCAAA+AGG + Chr4:83999832-83999851 MsG0480023339.01.T01:CDS 25.0%
!! AATTTTATCTCACAATCAGA+TGG - Chr4:83999471-83999490 None:intergenic 25.0%
!! AATTTTGTGAAGTGAAAAGT+GGG + Chr4:83999328-83999347 MsG0480023339.01.T01:intron 25.0%
!! ATCTTTGGAAATTTCAATAG+GGG - Chr4:83998489-83998508 None:intergenic 25.0%
!! CATAAAAATATAAAGGCATG+TGG + Chr4:83999358-83999377 MsG0480023339.01.T01:intron 25.0%
!! TAAAAATATAAAGGCATGTG+GGG + Chr4:83999360-83999379 MsG0480023339.01.T01:intron 25.0%
!!! TGCTAAGTATTTTGCTATTT+TGG + Chr4:83998595-83998614 MsG0480023339.01.T01:CDS 25.0%
ACAGACTTGTGAAACTATTT+GGG + Chr4:83998932-83998951 MsG0480023339.01.T01:intron 30.0%
AGTGATTTAGAAATGTGTAC+CGG - Chr4:83999632-83999651 None:intergenic 30.0%
ATAAAAGGTTCAAATGTGCA+GGG + Chr4:83999673-83999692 MsG0480023339.01.T01:intron 30.0%
ATACATTCTTGGCAATTCTA+AGG + Chr4:83999295-83999314 MsG0480023339.01.T01:intron 30.0%
ATGCTTTCATTGTTCCAATA+AGG + Chr4:83999875-83999894 MsG0480023339.01.T01:CDS 30.0%
ATGTTGCAACTAACTCAAAA+TGG - Chr4:83999400-83999419 None:intergenic 30.0%
GTTTATTCTATGCAGTCTTT+GGG + Chr4:83998746-83998765 MsG0480023339.01.T01:intron 30.0%
TACAGACTTGTGAAACTATT+TGG + Chr4:83998931-83998950 MsG0480023339.01.T01:intron 30.0%
TCAAAATGGGTGTTCAATAT+AGG + Chr4:83998650-83998669 MsG0480023339.01.T01:intron 30.0%
TCTACACTCTTAACATCAAT+TGG - Chr4:83999256-83999275 None:intergenic 30.0%
TGCTTTCATTGTTCCAATAA+GGG + Chr4:83999876-83999895 MsG0480023339.01.T01:CDS 30.0%
TTATAGGAGCAATACATTCT+TGG + Chr4:83999284-83999303 MsG0480023339.01.T01:intron 30.0%
TTTATTCTATGCAGTCTTTG+GGG + Chr4:83998747-83998766 MsG0480023339.01.T01:intron 30.0%
! AAAAATATAAAGGCATGTGG+GGG + Chr4:83999361-83999380 MsG0480023339.01.T01:intron 30.0%
! ACTCCTTTTGCATCATAATT+AGG - Chr4:83999838-83999857 None:intergenic 30.0%
! ATTGTGTATGAATCAGCTTT+TGG + Chr4:83998520-83998539 MsG0480023339.01.T01:CDS 30.0%
! CAATTTTGTGAAGTGAAAAG+TGG + Chr4:83999327-83999346 MsG0480023339.01.T01:intron 30.0%
! TGATGTTAAGAGTGTAGAAT+GGG + Chr4:83999258-83999277 MsG0480023339.01.T01:intron 30.0%
! TTGATGTTAAGAGTGTAGAA+TGG + Chr4:83999257-83999276 MsG0480023339.01.T01:intron 30.0%
!! CTATGATGGTATTGATAACT+TGG + Chr4:83998815-83998834 MsG0480023339.01.T01:CDS 30.0%
!! GTTCTTAAAGTGTTGTCATT+AGG + Chr4:83999213-83999232 MsG0480023339.01.T01:intron 30.0%
AAATTTCCAACAGAGACACT+AGG - Chr4:83999523-83999542 None:intergenic 35.0%
ACGAAGTAGAGAATATCTGA+TGG - Chr4:84000184-84000203 None:intergenic 35.0%
AGAATATCTGATGGCAATAG+TGG - Chr4:84000175-84000194 None:intergenic 35.0%
AGTGTCTCTGTTGGAAATTT+AGG + Chr4:83999523-83999542 MsG0480023339.01.T01:intron 35.0%
CAATCATGTATCTCAATTCC+AGG - Chr4:83999925-83999944 None:intergenic 35.0%
CAATTCTCGGTGATTATCTT+TGG - Chr4:83998504-83998523 None:intergenic 35.0%
CATAAAAGGTTCAAATGTGC+AGG + Chr4:83999672-83999691 MsG0480023339.01.T01:intron 35.0%
CATAAGGACAAGTACTATGA+TGG + Chr4:83998801-83998820 MsG0480023339.01.T01:CDS 35.0%
CATGATTGTGGTCATAAAGT+TGG + Chr4:83999937-83999956 MsG0480023339.01.T01:CDS 35.0%
GCAAAAGGAGTTTCTGATAT+GGG + Chr4:83999847-83999866 MsG0480023339.01.T01:CDS 35.0%
GGAATTGAGATACATGATTG+TGG + Chr4:83999925-83999944 MsG0480023339.01.T01:CDS 35.0%
GGTTTATTCTATGCAGTCTT+TGG + Chr4:83998745-83998764 MsG0480023339.01.T01:intron 35.0%
GTGATTTAGAAATGTGTACC+GGG - Chr4:83999631-83999650 None:intergenic 35.0%
TCGGTTTAGCAACTTCTATA+GGG + Chr4:83999186-83999205 MsG0480023339.01.T01:intron 35.0%
TGCAAAAGGAGTTTCTGATA+TGG + Chr4:83999846-83999865 MsG0480023339.01.T01:CDS 35.0%
TGCAACATCATTGTCGAATT+TGG + Chr4:83999412-83999431 MsG0480023339.01.T01:intron 35.0%
TTAGCAACTTCTATAGGGTT+GGG + Chr4:83999191-83999210 MsG0480023339.01.T01:intron 35.0%
TTATTAAGTGCCTACAGCAT+AGG - Chr4:83999107-83999126 None:intergenic 35.0%
TTTAGCAACTTCTATAGGGT+TGG + Chr4:83999190-83999209 MsG0480023339.01.T01:intron 35.0%
TTTGGGAACACAGAAACATA+AGG + Chr4:83998785-83998804 MsG0480023339.01.T01:CDS 35.0%
! AAGTGTTGTCATTAGGTTCA+TGG + Chr4:83999220-83999239 MsG0480023339.01.T01:intron 35.0%
! AGTGTAGAATGGGTTCTTAT+AGG + Chr4:83999268-83999287 MsG0480023339.01.T01:intron 35.0%
! ATGTCGCTTGGAATCAAAAT+GGG + Chr4:83998637-83998656 MsG0480023339.01.T01:CDS 35.0%
! ATTGATAACTTGGATTACCC+AGG + Chr4:83998825-83998844 MsG0480023339.01.T01:CDS 35.0%
! CATTTGATAGCACCATCATT+TGG - Chr4:83999754-83999773 None:intergenic 35.0%
! GCTGATTCATACACAATTCT+CGG - Chr4:83998517-83998536 None:intergenic 35.0%
! GGTGGTTCTGTAATCAATTT+GGG + Chr4:83998768-83998787 MsG0480023339.01.T01:CDS 35.0%
! TATGTCGCTTGGAATCAAAA+TGG + Chr4:83998636-83998655 MsG0480023339.01.T01:CDS 35.0%
! TGAGCTTTGTCTTATTCTGT+CGG + Chr4:83999167-83999186 MsG0480023339.01.T01:intron 35.0%
!! AATTCCAGGAAGTGTTTGAT+GGG - Chr4:83999911-83999930 None:intergenic 35.0%
!! CAAATGATGGTGCTATCAAA+TGG + Chr4:83999752-83999771 MsG0480023339.01.T01:CDS 35.0%
!!! TTTGCTATTTTGGAAGCTGT+TGG + Chr4:83998605-83998624 MsG0480023339.01.T01:CDS 35.0%
CAATTCAAACAAGCTCAGAG+AGG - Chr4:83998887-83998906 None:intergenic 40.0%
CACGAGAAACATCTCCAAAA+CGG - Chr4:84000032-84000051 None:intergenic 40.0%
CATCTCCAAAACGGTTTAGA+AGG - Chr4:84000023-84000042 None:intergenic 40.0%
CGCTTACAGAAGAATATGCA+AGG - Chr4:84000137-84000156 None:intergenic 40.0%
CTCCATTATCAACACCATTG+AGG - Chr4:83999963-83999982 None:intergenic 40.0%
CTTCAACCTAGCAAAAACTG+TGG - Chr4:83999812-83999831 None:intergenic 40.0%
GAAATTACCATGGTGAAGCT+CGG - Chr4:83998865-83998884 None:intergenic 40.0%
GTAGTCGAGAATCGTTACTT+CGG - Chr4:84000232-84000251 None:intergenic 40.0%
GTTGGAAATTTAGGTGCACT+GGG + Chr4:83999532-83999551 MsG0480023339.01.T01:intron 40.0%
TAAGCTTATGCTGATGAGAC+TGG - Chr4:84000254-84000273 None:intergenic 40.0%
TAGTCGAGAATCGTTACTTC+GGG - Chr4:84000231-84000250 None:intergenic 40.0%
TCAATAGGGGTTAGAAGATG+TGG - Chr4:83998476-83998495 None:intergenic 40.0%
TCTCTACTTCGTCAGCAATT+TGG + Chr4:84000192-84000211 MsG0480023339.01.T01:CDS 40.0%
TGGGTCTTCATATCCCTTAT+TGG - Chr4:83999892-83999911 None:intergenic 40.0%
TGTTGGAAATTTAGGTGCAC+TGG + Chr4:83999531-83999550 MsG0480023339.01.T01:intron 40.0%
TTCATAAGCACCTATGCTGT+AGG + Chr4:83999094-83999113 MsG0480023339.01.T01:intron 40.0%
TTGGAGATGTTTCTCGTGAT+GGG + Chr4:84000034-84000053 MsG0480023339.01.T01:CDS 40.0%
TTTGGAGATGTTTCTCGTGA+TGG + Chr4:84000033-84000052 MsG0480023339.01.T01:CDS 40.0%
! AGTTTGCCACAGTTTTTGCT+AGG + Chr4:83999803-83999822 MsG0480023339.01.T01:CDS 40.0%
! CTCTCTGAGCTTGTTTGAAT+TGG + Chr4:83998885-83998904 MsG0480023339.01.T01:intron 40.0%
! GACAACCTTCTAAACCGTTT+TGG + Chr4:84000015-84000034 MsG0480023339.01.T01:CDS 40.0%
! GGGTGGTTCTGTAATCAATT+TGG + Chr4:83998767-83998786 MsG0480023339.01.T01:CDS 40.0%
! GTCGGTTTAGCAACTTCTAT+AGG + Chr4:83999185-83999204 MsG0480023339.01.T01:intron 40.0%
! GTGTTTTCCTCTCATGTAAC+CGG - Chr4:83998411-83998430 None:intergenic 40.0%
!! ATGATGGTGCTATCAAATGG+TGG + Chr4:83999755-83999774 MsG0480023339.01.T01:CDS 40.0%
!! CAATTCCAGGAAGTGTTTGA+TGG - Chr4:83999912-83999931 None:intergenic 40.0%
AAGACCCATCAAACACTTCC+TGG + Chr4:83999904-83999923 MsG0480023339.01.T01:CDS 45.0%
CCTTCGGTTAATAAGCGGTT+CGG + Chr4:84000075-84000094 MsG0480023339.01.T01:CDS 45.0%
CGCTTCCTTCGGTTAATAAG+CGG + Chr4:84000070-84000089 MsG0480023339.01.T01:CDS 45.0%
CGGTCATAGCAAAACAACCT+GGG - Chr4:83998845-83998864 None:intergenic 45.0%
GCTCAGAGAGGAAATTACCA+TGG - Chr4:83998875-83998894 None:intergenic 45.0%
GTACACAAGTACGCTTCCTT+CGG + Chr4:84000059-84000078 MsG0480023339.01.T01:CDS 45.0%
TCGCGGGAAGAAATTAATCG+TGG + Chr4:83998370-83998389 MsG0480023339.01.T01:CDS 45.0%
TCGGTCATAGCAAAACAACC+TGG - Chr4:83998846-83998865 None:intergenic 45.0%
TGTAAGCGTCCTCAAGGTTT+CGG + Chr4:84000149-84000168 MsG0480023339.01.T01:CDS 45.0%
TTCATCGGTGATCGGATCAA+AGG - Chr4:83999716-83999735 None:intergenic 45.0%
TTCTTCTGTAAGCGTCCTCA+AGG + Chr4:84000143-84000162 MsG0480023339.01.T01:CDS 45.0%
TTTCCAACAGAGACACTAGG+AGG - Chr4:83999520-83999539 None:intergenic 45.0%
! ATTCTATGCAGTCTTTGGGG+TGG + Chr4:83998750-83998769 MsG0480023339.01.T01:intron 45.0%
! GCATAAGTTGAAGCCAGCAT+GGG - Chr4:84000279-84000298 None:intergenic 45.0%
! GGTCATAAAGTTGGCCTCAA+TGG + Chr4:83999946-83999965 MsG0480023339.01.T01:CDS 45.0%
! TGCATAAGTTGAAGCCAGCA+TGG - Chr4:84000280-84000299 None:intergenic 45.0%
! TGGTAGTGTGATATGTCGCT+TGG + Chr4:83998625-83998644 MsG0480023339.01.T01:CDS 45.0%
! TTGGCTCTTGTTAGAGAAGC+TGG + Chr4:83998539-83998558 MsG0480023339.01.T01:CDS 45.0%
!! GGCCTCAATGGTGTTGATAA+TGG + Chr4:83999958-83999977 MsG0480023339.01.T01:CDS 45.0%
!! GGTGCTATCAAATGGTGGAT+TGG + Chr4:83999760-83999779 MsG0480023339.01.T01:CDS 45.0%
!! TAAAGCGGTGTCGCATAGTA+TGG + Chr4:83999444-83999463 MsG0480023339.01.T01:intron 45.0%
AACAGAGACACTAGGAGGCT+AGG - Chr4:83999515-83999534 None:intergenic 50.0%
ATCGGTGATCGGATCAAAGG+TGG - Chr4:83999713-83999732 None:intergenic 50.0%
CAAAGGTGGCGAGAGTTTGA+AGG - Chr4:83999699-83999718 None:intergenic 50.0%
CAATAGTGGCCGAAACCTTG+AGG - Chr4:84000161-84000180 None:intergenic 50.0%
CCGAACCGCTTATTAACCGA+AGG - Chr4:84000078-84000097 None:intergenic 50.0%
CGAGAATCGTTACTTCGGGT+TGG - Chr4:84000227-84000246 None:intergenic 50.0%
CGGTTTAGAAGGTTGTCTCG+AGG - Chr4:84000012-84000031 None:intergenic 50.0%
GCATAAGCTTATGCCCATGC+TGG + Chr4:84000263-84000282 MsG0480023339.01.T01:CDS 50.0%
GCTACTGCATAGCACTTACC+CGG + Chr4:83999610-83999629 MsG0480023339.01.T01:intron 50.0%
GTGGACACAAATTCCCTCTC+CGG + Chr4:83998389-83998408 MsG0480023339.01.T01:CDS 50.0%
TAGCCTCCTAGTGTCTCTGT+TGG + Chr4:83999514-83999533 MsG0480023339.01.T01:intron 50.0%
TCCTCTCATGTAACCGGAGA+GGG - Chr4:83998405-83998424 None:intergenic 50.0%
TGGAGCTCTTAGATTCCGCT+CGG + Chr4:83999978-83999997 MsG0480023339.01.T01:CDS 50.0%
TGTGGCAAACTTGCCATGCA+TGG - Chr4:83999794-83999813 None:intergenic 50.0%
TTCCTCTCATGTAACCGGAG+AGG - Chr4:83998406-83998425 None:intergenic 50.0%
!! GAACTTGTTGGTGGTAGGGT+AGG + Chr4:84000111-84000130 MsG0480023339.01.T01:CDS 50.0%
ATTGGCAATGCCGCCATGCA+TGG + Chr4:83999778-83999797 MsG0480023339.01.T01:CDS 55.0%
CTCGAGGAATTCTCACCGAG+CGG - Chr4:83999996-84000015 None:intergenic 55.0%
GCAACTCTCGGGGAACTTGT+TGG + Chr4:84000099-84000118 MsG0480023339.01.T01:CDS 55.0%
GCTATGACCGAGCTTCACCA+TGG + Chr4:83998855-83998874 MsG0480023339.01.T01:CDS 55.0%
GGCAAACTTGCCATGCATGG+CGG - Chr4:83999791-83999810 None:intergenic 55.0%
TCCCTCTCCGGTTACATGAG+AGG + Chr4:83998401-83998420 MsG0480023339.01.T01:CDS 55.0%
!! AAGCGGTTCGGTGCAACTCT+CGG + Chr4:84000087-84000106 MsG0480023339.01.T01:CDS 55.0%
!! ACTCTCGGGGAACTTGTTGG+TGG + Chr4:84000102-84000121 MsG0480023339.01.T01:CDS 55.0%
!! CGGGGAACTTGTTGGTGGTA+GGG + Chr4:84000107-84000126 MsG0480023339.01.T01:CDS 55.0%
!! GTTACTTCGGGTTGGTCTCG+AGG - Chr4:84000219-84000238 None:intergenic 55.0%
!! TCGGGGAACTTGTTGGTGGT+AGG + Chr4:84000106-84000125 MsG0480023339.01.T01:CDS 55.0%
AGGCATGTGGGGGTAACGTG+TGG + Chr4:83999371-83999390 MsG0480023339.01.T01:intron 60.0%
GGCATGTGGGGGTAACGTGT+GGG + Chr4:83999372-83999391 MsG0480023339.01.T01:intron 60.0%
!! AGCGGTTCGGTGCAACTCTC+GGG + Chr4:84000088-84000107 MsG0480023339.01.T01:CDS 60.0%
!! GCGGTTCGGTGCAACTCTCG+GGG + Chr4:84000089-84000108 MsG0480023339.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 83998356 84000323 83998356 ID=MsG0480023339.01;Name=MsG0480023339.01
Chr4 mRNA 83998356 84000323 83998356 ID=MsG0480023339.01.T01;Parent=MsG0480023339.01;Name=MsG0480023339.01.T01;_AED=0.26;_eAED=0.26;_QI=0|0.83|0.85|1|0.83|0.71|7|0|354
Chr4 exon 83998356 83998660 83998356 ID=MsG0480023339.01.T01:exon:6027;Parent=MsG0480023339.01.T01
Chr4 exon 83998761 83998876 83998761 ID=MsG0480023339.01.T01:exon:6028;Parent=MsG0480023339.01.T01
Chr4 exon 83999680 84000323 83999680 ID=MsG0480023339.01.T01:exon:6029;Parent=MsG0480023339.01.T01
Chr4 CDS 83998356 83998660 83998356 ID=MsG0480023339.01.T01:cds;Parent=MsG0480023339.01.T01
Chr4 CDS 83998761 83998876 83998761 ID=MsG0480023339.01.T01:cds;Parent=MsG0480023339.01.T01
Chr4 CDS 83999680 84000323 83999680 ID=MsG0480023339.01.T01:cds;Parent=MsG0480023339.01.T01
Gene Sequence

>MsG0480023339.01.T01

ATGGAAAATTGCAGTCGCGGGAAGAAATTAATCGTGGACACAAATTCCCTCTCCGGTTACATGAGAGGAAAACACAGAGAGATACAAGAGAAAATTTTCGATTACTTCAACGCTAATCCACATCTTCTAACCCCTATTGAAATTTCCAAAGATAATCACCGAGAATTGTGTATGAATCAGCTTTTGGCTCTTGTTAGAGAAGCTGGAGTTAAACCTCTTCATTATGTTGTTAATGATCCTGCTAAGTATTTTGCTATTTTGGAAGCTGTTGGTAGTGTGATATGTCGCTTGGAATCAAAATGGGTTCTTTGGGGTGGTTCTGTAATCAATTTGGGAACACAGAAACATAAGGACAAGTACTATGATGGTATTGATAACTTGGATTACCCAGGTTGTTTTGCTATGACCGAGCTTCACCATGGTTCAAATGTGCAGGGCCTTCAAACTCTCGCCACCTTTGATCCGATCACCGATGAATTTATTATTGATACTCCAAATGATGGTGCTATCAAATGGTGGATTGGCAATGCCGCCATGCATGGCAAGTTTGCCACAGTTTTTGCTAGGTTGAAGTTACCTAATTATGATGCAAAAGGAGTTTCTGATATGGGTGTTCATGCTTTCATTGTTCCAATAAGGGATATGAAGACCCATCAAACACTTCCTGGAATTGAGATACATGATTGTGGTCATAAAGTTGGCCTCAATGGTGTTGATAATGGAGCTCTTAGATTCCGCTCGGTGAGAATTCCTCGAGACAACCTTCTAAACCGTTTTGGAGATGTTTCTCGTGATGGGAAGTACACAAGTACGCTTCCTTCGGTTAATAAGCGGTTCGGTGCAACTCTCGGGGAACTTGTTGGTGGTAGGGTAGGCCTTGCATATTCTTCTGTAAGCGTCCTCAAGGTTTCGGCCACTATTGCCATCAGATATTCTCTACTTCGTCAGCAATTTGGACCTCGAGACCAACCCGAAGTAACGATTCTCGACTACCAGTCTCATCAGCATAAGCTTATGCCCATGCTGGCTTCAACTTATGCATTTCATTTTGCCACACAAACTTAG
Protein sequence

>MsG0480023339.01.T01

MENCSRGKKLIVDTNSLSGYMRGKHREIQEKIFDYFNANPHLLTPIEISKDNHRELCMNQLLALVREAGVKPLHYVVNDPAKYFAILEAVGSVICRLESKWVLWGGSVINLGTQKHKDKYYDGIDNLDYPGCFAMTELHHGSNVQGLQTLATFDPITDEFIIDTPNDGAIKWWIGNAAMHGKFATVFARLKLPNYDAKGVSDMGVHAFIVPIRDMKTHQTLPGIEIHDCGHKVGLNGVDNGALRFRSVRIPRDNLLNRFGDVSRDGKYTSTLPSVNKRFGATLGELVGGRVGLAYSSVSVLKVSATIAIRYSLLRQQFGPRDQPEVTILDYQSHQHKLMPMLASTYAFHFATQT*